日度归档：2026年4月15日

白藜芦醇及其相关产品

发表于由

产品编号 产品名称 产品规格 产品等级 产品价格
184-02771 Resveratrol, Synthetic
 白藜芦醇		 1G Wako Special Grade –
180-02773 Resveratrol, Synthetic
 白藜芦醇		 5G Wako Special Grade –
182-02772 Resveratrol, Synthetic
 白藜芦醇		 25G Wako Special Grade –
070-06141 Gnetin C Standard
反式白藜芦醇二聚体标准品		 50mg for Food Analysis –
226-02021 ε-Viniferin Standard
 ε-葡萄素标准品		 20mg for Food Analysis –

白藜芦醇及其相关产品

　　

　　白藜芦醇，一种在葡萄皮和其他可食用的植物和相关的食物产品中发现的多酚，是一种抗氧化剂。最近，各种白藜芦醇有益于健康的好处已经被广泛研究。我们还供应反式白藜芦醇二聚体和ε‐葡萄素标准品，即白藜芦醇二聚体。

◆白藜芦醇


中文名称

英文名称

等级

包装（g）

货号

储存条件

 

白藜芦醇

 

Resveratrol,Synthetic

 

和光特级

1

184-02771

 

‐20 ˚C保存

5

180-02773

25

18202772

检测：98±%（HPLC）

外观：白色~浅棕色，结晶性粉末~粉末状或块状                  

化学名称：5‐[（1）2（4‐‐‐羟基苯基）乙烯基]‐‐1,3-苯二酚

CAS NO.：501-36-0

化学结构式：

白藜芦醇及其相关产品

 


◆反式白藜芦醇二聚体标准品


中文名称

英文名称

等级

包装（mg）

货号

储存条件

反式白藜芦醇二聚体

Gnetin   C Standard

食品分析用

50mg

070-06141

 ‐20 ˚C保存

检测：97±%（HPLC）

外观：白色~土黄色，结晶性粉末~粉末或者块状

化学名称：REL‐5‐[（2 R，3 R）‐2,3‐二氢‐4‐羟基‐2‐（4‐羟基苯基）‐6‐[（1）2（4‐‐‐羟基苯基）乙烯基]苯并呋喃基]‐3‐‐1,3‐苯二酚

注：反式白藜芦醇暴露于荧光或自然光时，可能会分解

CAS NO.：84870-54-2

化学结构式：

白藜芦醇及其相关产品



◆ε‐葡萄素标准品


中文名称

英文名称

等级

包装（mg）

货号

储存条件

ε‐葡萄素标准品

ε‐Viniferin   Standard

食品分析用

20mg

226-02021

 ‐20 ˚C保存

检测：98±%（HPLC）

外观：白色~浅灰棕色，结晶性粉末~粉末或者块状

化学名称：5‐[（2R，3R）‐2,3‐二氢‐6‐羟基‐2‐（4‐羟基苯基）‐4‐[（1E）‐2‐（4‐羟基苯基）乙烯基]苯并呋喃基]‐3‐‐1,3‐苯二酚

注：反式白藜芦醇二聚体包含在绿芽和葡萄藤中

CAS NO.：62218-08-0

化学结构式：

白藜芦醇及其相关产品

◆产品列表


产品编号

产品名称

产品规格

184-02771

Resveratrol, Synthetic

白藜芦醇

1G

180-02773

Resveratrol, Synthetic

白藜芦醇

5G

182-02772

Resveratrol, Synthetic

白藜芦醇

25G

070-06141

Gnetin C Standar

d反式白藜芦醇二聚体标准品

50mg

226-02021

ε-Viniferin Standar

dε-葡萄素标准品

20mg

◆相关产品


产品编号

产品名称

产品规格

182-02831

Resveratrol Standard

白藜芦醇标准品

100 mg

◆其他食品分析用标准品


中文名称

英文名称

等级

包装

货号

储存条件

备注

薯蓣皂素标准品

Diosgenin   Std.,

98.0+%(HPLC)

食品分析用

100mg

043-32621

2~10℃保存

一种含在山药中的孕酮同源物

白藜芦醇

Resvertrol,

98.0+%(HPLC)

生物化学用

100mg

185-01721

‐20   ˚C保存

自然提取

500mg

181-01723

杜克甙A标准品

Dulcoside   Std,

98.0+%(HPLC,)

食品分析用

25mg

048-31211

2~10℃保存

 

 

 

 

 

 

 

 

 

 

甜叶菊中甜味剂

 

100mg

044-31213

异甜菊苷标准品

Isosteviol   Std.,

98.0+%(HPLC,)

测定甜菊糖用

1g

098-05681

2~10℃保存

莱苞迪甙A标准品

Rebaudioside   A Std.,
  99.0+% (HPLC)

食品分析用

100mg

189-02581

2~10℃保存

1g

185-02583

莱苞迪甙B标准品

Rebaudioside   B Std.,
  98.0+% (HPLC)

食品分析用

25mg

188-02551

2~10℃保存

100mg

184-02553

莱苞迪苷C

Rebaudioside   C,

90.0+%   (HPLC)

食品分析用

25mg

181-02541

2~10℃保存

莱苞迪苷D

Rebaudioside   D

食品分析用

5mg

180-02511

‐20   ˚C保存

莱苞迪苷F

Rebaudioside   F

食品分析用

5mg

186-02611

2~10℃保存

甜茶苷标准品

Rubusoside   Std.,
  98.0+ % (HPLC)

食品分析用

25mg

187-02521

‐20   ˚C保存

100mg

183-02523

甜菊糖标准品

Steviol   Std.,
  99.0+ % (HPLC)

食品分析用

25mg

192-15701

2~10℃保存

100mg

198-15703

甜菊双糖甙标准品

Steviolbioside   Std.,
  98.0+ % (HPLC)

食品分析用

25mg

199-15691

2~10℃保存

100mg

195-15693

甜叶菊甙元标准品

Stevioside   Std.,

99.0+   % (HPLC)

食品分析用

100mg

199-16291

2~10℃保存

 

Lumiprobe品牌代理商

发表于由

Lumiprobe品牌代理商–上海金畔

1. Lumiprobe品牌介绍
Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活
性荧光染料。从2006年开始，公司生产并销售生命科学研究和诊断学应用的优质化学药品。
产品主要有：活性染料(Reactive Dye)和SYBR Green I 染料，用于寡核苷酸合成的亚
磷酰胺，点击化学和其它试剂。产品主要应用：点击化学(Click Chemistry)、蛋白质组学
研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。产品包括
：N-羟基琥珀酰亚胺酯类活性染料；羰基活性荧光染料；氨基类染料 ；巯基反应性染料；
羧酸类染料。
2. Lumiprobe重点产品
N-羟基琥珀酰亚胺酯类活性染料；羰基活性荧光染料；氨基类染料 ；巯基反应性染料；羧
酸类染料。
3. Lumiprobe官网
http://www.lumiprobe.com/

4. 金畔生物代理Lumiprobe品牌联系方式
上海金畔生物科技有限公司
地 址： 上海市浦东新区东靖路699弄36栋701室
邮 编： 201208
qq: 2743691513
固话总机：021-50837765
订货热线：15221999938
网 址： www.jinpanbio.com
Email：sales@jinpanbio.com

Wako  和光纯药 SuperSep™ Phos-tag™ (50μmol/l), 6%, 13well Phos-tag™ 预制胶50μmol/l，6%，13孔

发表于由

Wako  和光纯药 SuperSep™ Phos-tag™ (50μmol/l), 6%, 13well Phos-tag™ 预制胶50μmol/l，6%，13孔

Wako Wako ——————和光纯药 192-17401
SuperSep™ Phos-tag™ (50μmol/l), 6%, 13well
Phos-tag™ 预制胶50μmol/l，6%，13孔
– 5块 3,370.00 -Wako ——————和光纯药 193-16711
SuperSep™ Phos-tag™ (50 μmol/L), 10%,13 well
Phos-tag™ 预制胶50μmol/l，10%，13孔
for Electrophoresis 5 EA 3,370.00 (9003-05-8)Wako ——————和光纯药 195-17371
SuperSep™ Phos-tag™ (50μmol/l), 7.5%, 13well
Phos-tag™ 预制胶50μmol/l，7.5%，13孔
– 5块 3,370.00 -Wako ——————和光纯药 199-17391
SuperSep™ Phos-tag™ (50μmol/l), 6%, 17well
Phos-tag™ 预制胶50μmol/l，6%，17孔
– 5块 3,370.00 -Wako ——————和光纯药 196-16701
SuperSep™ Phos-tag™ (50 μmol/L), 15%,17 well
Phos-tag™ 预制胶50μmol/l，15%，17孔
– 5块 3,370.00 -Nard ——————- 300-93523
Phos-tag™ Acrylamide AAL-107
Phos-tag™ 丙烯酰胺
– 2 mg 3,060.00

重组无细胞蛋白合成系统

发表于由

产品编号 产品名称 产品规格 产品等级 产品价格
GFK-PF201-0.25-EX PUREfrex® 2.0 1KIT － －
GFK-PF201-0.25-5-EX PUREfrex® 2.0 1KIT － －
GFK-PF213-0.25-EX PUREfrex® 2.1 1KIT － －
GFK-PF213-0.25-5-EX PUREfrex® 2.1 1KIT － －
GFK-PF003-0.5-EX DnaK Mix 1KIT － －
GFK-PF004-0.5-EX GroE Mix 1KIT
GFK-PF005-0.5-EX DS supplement 1KIT

重组合无细胞蛋白合成系统重组无细胞蛋白合成系统

PUREfrex® 2.0

 



◆简介


　　PUREfrex® 试剂盒是在东京大学的Takuya Ueda教授所发明的PUREsystem技术基础上，新开发的一款重组合无细胞蛋白合成试剂盒。

　　反应系统由蛋白质、核糖体、氨基酸和NTPs组成[1,2]，其中蛋白行使转录、翻译和能量供应的功能。蛋白与核糖体为分别单独高度纯化后，再重新组合成蛋白合成系统，而非直接从大肠杆菌S30中提取。当合成蛋白时，仅需将编码目的蛋白的模板DNA或mRNA添加到反应混合液中并孵育数小时，即可完成反应。本系统最大的特色，是在体外以转录相关因子重新组合一套表达系统，并可根据需要来调整反应混合物的成分，而不必担心高背景会影响下游的应用。进行蛋白表达仅需将编码目标蛋白的模板DNA或mRNA加入到反应体系中，然后孵育2-4小时即可完成反应。PUREfrex® 试剂盒的所有蛋白组分均不带标签，因此目的蛋白可融合任意标签进行纯化和检测。

重组无细胞蛋白合成系统

 


>>>无细胞表达的优势<<<


●　无需制备克隆

●　无需考虑培养条件

●　无需考虑表达所需的诱导条件

●　来源于宿主的污染少

 



◆PUREfrex® 系列


●　PUREfrex® 1.0 第一代产品    

●　PUREfrex® 2.0 第二代产品，表达量更高，污染水平更低；

●　RNA酶与β-半乳糖苷酶污染大大降低；

●　每1 µL反应混合物中的脂多糖（LPS）低于0.1 EU。

●　PUREfrex® 2.1 更适合二硫键的形成



☆升级至PUREfrex® 2.0


1．合成原核和真核蛋白的结果显示，用PUREfrex® 2.0合成时，各种蛋白的合成量增加。

重组无细胞蛋白合成系统

2．GFP蛋白合成的结果显示，用PUREfrex® 2.0合成时，可以观察到荧光强度增强了

2．10倍以上（每单位反应产物）。

重组无细胞蛋白合成系统

3．合成需要形成二硫键（SS键）的大肠杆菌酸性磷酸酶（AppA1）时，在PUREfrex® 2.0基础上，

3．添加了DS supplement的结果显示，存在氧化剂和二硫键异构酶时，活性蛋白合成量增加。

重组无细胞蛋白合成系统

1：AppA有5个二硫键，是其中一个位点在不连续的半胱氨酸之间存在的二硫键。



4．正确高级结构蛋白的合成结果显示，存在分子伴侣2的情况下用PUREfrex® 2.0，蛋白合成量增加。

重组无细胞蛋白合成系统

2 DnaK Mix：DnaK / DnaJ / GrpE mixture为配套分子伴侣

 



◆特点


●　可以同时加入多种模板进行反应，以合成Fab（带二硫键）及多聚体等带二级结构的多肽

●　可合成活细胞难以合成的强毒性蛋白

●　可直接使用PCR产物来作为模板DNA

●　单位体积内合成的蛋白量几乎恒定，不随反应体积变化而产生显著差异

●　操作简便，仅需在37℃孵育数小时

●　可以合成带标签的蛋白用于下游纯化和检测

●　产品经优化升级，合成量大大提高

 



◆应用


制备目的蛋白


●　原核蛋白

●　真核蛋白

●　膜蛋白

●　二硫键蛋白

●　含有非天然氨基酸的蛋白质等

 


蛋白基础研究


●　翻译

●　蛋白合成后折叠

 


体外展示技术


●　核糖体展示技术

●　mRNA展示技术

 



◆应用实例


利用PUREfrex® 系统合成并一步纯化DHFR-His

重组无细胞蛋白合成系统

3：模板DNA的构建方法请见"相关资料"栏或点击这里。




◆添加剂（用于需要形成二硫键和分子伴侣的蛋白质）


●　DS supplement

●　通过添加DS supplement至PUREfrex® 反应液中，为二硫键形成创造最佳的环境。DS supplement作为创造氧化环境的氧化剂，含有氧化型谷胱甘肽（GSSG）和作为二硫键异构酶的大肠杆菌DsbC。当蛋白需要二硫键才能产生活性形式时，请使用本添加剂。

 

●　DnaK Mix

●　DnaK Mix是高度纯化后的大肠杆菌来源的DnaK、DnaJ、GrpE蛋白以适当的浓度比例预混后的溶液。在PUREfrex® 反应体系中单独或添加DS supplement合成蛋白时同时添加DnaK Mix，可以更易获得难以独自形成高级结构的活性蛋白。

 

●　GroE Mix

●　GroE Mix是高度纯化后的大肠杆菌来源的GroEL、GroES蛋白以适当的浓度比例预混后的溶液。以PUREfrex® 反应体系合成蛋白时添加GroE Mix，可以更易获得难以独自形成高级结构的活性蛋白。

 



◆试剂盒组成


用于250 μL反应

使用前请将试剂盒置于-80°C保存

试剂 体积 成分说明 保存温度
溶液 I （白盖） 125 μL 氨基酸，核苷酸，tRNA和酶的底物等 -20°C
溶液 II （黑盖） 12.5 μL 蛋白，保存于含30％甘油的缓冲液 -20°C or -80°C（1）
溶液 III （红盖） 12.5 μL ×2 核糖体（20 μM） -80°C（1）

DHFR   DNA （透明盖）（2）

 10 μL 对照DNA，含有编码大肠杆菌DHFR基因的PCR产物（20 ng/μL） -20°C

（1）剩余的溶液应快速在液氮、干冰或乙醇中冻结，并储存于-80℃。如有必要，分装剩余溶液，并尽可能避免反复冻融。

（2）每50 μL反应中加入2.5 μL DHFR DNA。

 

◆产品列表


产品编号

产品名称

规格

备注信息

GFK-PF201-0.25-EX

PUREfrex® 2.0

1 kit

供250 μL反应使用

GFK-PF201-0.25-5-EX

PUREfrex® 2.0

1 kit

供250 μL×5次反应使用

GFK-PF213-0.25-EX

PUREfrex® 2.1

1 kit

供250 μL反应使用

GFK-PF213-0.25-5-EX

PUREfrex® 2.1

1 kit

供250 μL×5次反应使用

GFK-PF003-0.5-EX

DnaK Mix

1 kit

供500 μL反应使用

GFK-PF004-0.5-EX

GroE Mix

1 kit

供500 μL反应使用

GFK-PF005-0.5-EX

DS supplement

1 kit

供500 μL反应使用

 

相关产品的详细信息请点击查看：重组无细胞蛋白合成系统 PUREfrex® 2.0

相关资料

重组无细胞蛋白合成系统 重组无细胞蛋白合成系统

PUREfrex™ Technical information

PUREfrex™ Protocol

蛋白质工程相关产品

PUREfrex：重组无细胞蛋白合成试剂盒

RYTS试剂盒：大肠杆菌无细胞蛋白质合成系统

CloverDirect:定点蛋白质功能化tRNA试剂

纯化系统:一步高纯度标记纯化系统

STELLA +“赖氨酸标记试剂盒”

  • PUREfrex : Reconstituted Cell-free Protein Synthesis Kit

  • RYTS Kit : E. coli Cell-free Protein Synthesis System

  • CloverDirect : tRNA Reagents for Site-Directed Protein Functionalization

  • Dock Purification System : One step high purity purification tag purification system

  • STELLA+ " Lysine Labeling Kit "

PUREfrex™ Q&A

Ｑ:　使用PUREfrex™ 试剂盒是否可用于真核蛋白的合成？

Ａ:　PUREfrex™ 是由E.coli的核糖体和翻译因子组成的体外重组蛋白合成试剂盒，但也可以合成哺乳动物和植物的蛋白。目标蛋白的合成效率取决于编码蛋白的核苷酸序列，比如GC含量，稀有密码子的含量。

 

Ｑ:　使用PUREfrex™ 试剂盒可以合成多少蛋白？

Ａ:　这个取决于目标蛋白。来自E.coli的二氢叶酸还原酶每毫升反应液可合成150ug。

 

Ｑ:　是否可以合成大于100kDa的蛋白？

Ａ:　我们用该试剂盒合成了116kDa的蛋白。

 

Ｑ:　是否可以推荐PUREfrex™ 的反应条件？

Ａ:　推荐用该试剂盒在37℃反应2~4小时。

 

Ｑ:　是否可以合成和纯化标签蛋白？

Ａ:　可以使用任何标签，PUREfrex™ 试剂盒的所有蛋白成分都没有用于纯化或者检测的标签。比如，合成后可用金属螯合的树脂纯化带有His标签的目标蛋白。

 

Ｑ:　合成蛋白是否经糖基化或者磷酸化修饰？

Ａ:　不。不会发生翻译后修饰，PUREfrex™ 试剂盒只是由翻译因子组成。

 

Ｑ:　PUREfrex™ 试剂盒是否含有分子伴侣？

Ａ:　不。PUREfrex™ 试剂盒不含有任何分子伴侣，但你可以添加分子伴侣，比如Hsp70。你可以自己制备。

 

Ｑ:　用PUREfrex™ 试剂盒是否可合成含有二硫键的蛋白？

Ａ:　不行。目标蛋白合成不带有二硫键，因为翻译反应时有还原剂DTT。大多数需要二硫键才有活性的蛋白，会没有活性。

 

Ｑ:　PUREfrex™ 是否可合成膜蛋白？

Ａ:　一般情况，合成膜蛋白会形成聚集。为了获得能够插入到脂双层的膜蛋白，需要在合成膜蛋白时添加脂质体到PUREfrex™。

 

Ｑ:　是否可合成带有[35S] 甲硫氨酸或者 [3H] 亮氨酸的蛋白？

Ａ:　添加放射性元素标记的氨基酸可以合成放射性元素标记的蛋白，比如[35S] 甲硫氨酸或者 [3H] 亮氨酸。PUREfrex™ 含有20种天然的氨基酸，浓度都在0.5mM。请优化条件。

 

Ｑ:　除了T7启动子外，是否可用其他启动子？

Ａ:　我们推荐使用T7启动子的模板DNA，因为PUREfrex™ 含有转录的RNA聚合酶。当你使用其他聚合酶，制备的模板DNA要有相应聚合酶的合适启动子。

 

Ｑ:　使用DHFR DNA(阳性对照)无法获得DHFR。

Ａ:　该试剂盒由于某些原因失活。为了避免失活，请将该试剂盒存放在适当稳定。可进行分装，避免反复冻融影响试剂盒的使用效果。或者改试剂盒被核酸酶污染了。请使用不含核酸酶的水，试剂和材料。

 

Ｑ:　使用试剂盒的DHFR可以得到DHFR。但是不能得到目标蛋白，或者目标蛋白量很低。

Ａ:　1）改试剂盒由于某些原因失活了。为了避免失活，请将该试剂盒存放在适当的温度并且进行分装（避免反复冻融）

Ａ:　2）可以受核酸酶污染。为了避免核酸酶污染，请使用不含核酸酶的水，试剂和材料。

Ａ:　3）制备的DNA模板不准确。需要制备含有T7启动子，核糖体结合位点，起始密码子，终止密码子的DNA模板。

Ａ:　4）转录的二级结构会阻止翻译反应。这种情况，请优化模板的顺序，解决二级结构的问题。

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葡萄糖苷酸酶检测底物 Aldouronic Acid Mixture (Reduced) 货号：O-AMXR Megazyme中文站

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葡萄糖苷酸酶检测底物

英文名：Aldouronic Acid Mixture (Reduced)

货号：O-AMXR

规格：100 mg

High purity Aldouronic Acids Mixture (NaBH4 Reduced) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Contains a mixture of borohydride reduced aldobiouronic, aldotriouronic and aldotetraouronic and aldopentaouronic acids (10:60:20:10). Substrate for α-glucuronidase.

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抗结蛋白抗体[ y66 ] – Cytoskeleton Marker（ab32362）

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种属反应性

与反应：小鼠、大鼠、几内亚猪、人
  • 应用 AB评论 说明 国际商会/如果 1 / 50。 WB 1 / 100000。预测分子量：分子量为53 kDa。 ihc-p 2000 / 1。进行热介导抗原修复免疫组化染色协议开始之前。

    见协议（链路：http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol）。

    IHC -平片 用于测定浓度依赖性。考研：21856924 流式细胞 1 / 70。

    ab172730兔单克隆抗体，适用于该抗体的同型对照。

    免疫组化法 用于测定浓度依赖性。考研：21824387
  • 应用说明不适合IP。

    • 靶标

    • 功能结蛋白III类中间纤维在肌肉细胞中发现的。成人横纹肌形成纤维网络连接的肌原纤维彼此的质膜从Z线结构的外围。
    • 疾病相关缺陷在DES是肌病肌原纤维结蛋白相关的原因（mfm-des）[ 601419 ]；MIM：也称为结蛋白相关肌病（DRM）。神经肌肉疾病，骨骼肌肉无力，心脏传导阻滞、心律失常有关，限制性心脏衰竭，和肌原纤维破坏反应结蛋白沉积在心脏和骨骼肌细胞胞浆内的积累。缺陷在DES是心肌病引起扩张型1I（cmd1i）[ 604765 ] MIM。扩张型心肌病是一种以心室扩张和收缩功能受损的疾病，导致充血性心力衰竭和心律失常。患者的过早死亡风险。缺陷在DES的神经源性scapuloperoneal综合征原因（Kaeser凯撒型综合征）：[ 181400 ] MIM。凯撒综合征是一种常染色体显性遗传性疾病，有特殊的无力和萎缩scapuloperoneal分布。大量临床变化观察从scapuloperoneal，变的心脏或呼吸系统受累肢体远端弓鳍鱼和表型。面瘫，常见的附加症状是吞咽困难和男性乳房发育症。受影响的人似乎承担较高风险突发心脏死亡相比，受影响的妇女。肌肉活检标本的组织学和免疫组织化学检查显示广泛的研究范围从接近正常或非特异性病理改变典型，肌原纤维与积累的结蛋白的变化。
    • 序列相似性属于中间丝家族。
    • 细胞定位细胞质.