OECD培养基,浓缩液Ⅰ~Ⅳ


产品编号 产品名称 产品规格 产品等级 产品价格
158-03315 OECD Medium, Stock Solution
OECD培养基, 浓缩液
500mL 植物培养用
153-03321 OECD培地, 浓縮液Ⅱ(×1,000) 50mL 植物培养用
150-03331 OECD培地, 浓縮液Ⅲ(×1,000) 50mL 植物培养用
157-03341 OECD培地, 浓縮液Ⅳ(×1,000) 50mL 植物培养用

OECD培养基,浓缩液Ⅰ~ⅣOECD培养基, 浓缩液Ⅰ~Ⅳ

利用淡水藻类进行抑制生长试验



OECD培养基,浓缩液Ⅰ~Ⅳ

   WET(Whole Effluent Toxicity)是由美国研发的利用生物应答的水环境管理方法。本产品是WET法之一“淡水藻类生长抑制试验”的培养基配制用浓缩液。通过混合、稀释本产品,可以根据OECD TEST GUIDELINE No.201配制培养基。

 

OECD 培养基, 浓缩液Ⅰ~Ⅳ成分表


产品编号

规格

成分

浓度(mg/L) (原液)

浓度(mg/L) (稀释后)

158-03315

500 mL

OECD Medium,
     Stock Solution    I(×100)

×100

×1

NH4Cl

1,500.00

15.0000

MgCl2, 6H2O

1,200.00

12.0000

CaCl2, 2H2O

1,800.00

18.0000

MgSO4, 7H2O

1,500.00

15.0000

KH2PO4

 160.00

 1.6000

153-03321

50 mL

OECD Medium,
     Stock Solution Ⅱ(×1,000)

×1,000

×1

FeCl3, 6H2O

 64.00

0.0640

EDTA-Na2, 2H2O

100.00

0.1000

150-03331

50 mL

OECD Medium,
     Stock Solution Ⅲ(×1,000)

×1,000

×1

H3BO3

185.00

0.1850

MnCl2, 4H2O

415.00

0.4150

ZnCl2

  3.00

0.0030

CoCl2, 6H2O

  1.50

0.0015

CuCl2, 2H2O

  0.01

 0.00001

Na2MoO4,2H2O

  7.00

0.0070

157-03341

50 mL

OECD Medium,
     Stock Solution Ⅳ(×1,000)

×1,000

×1

NaHCO3

50,000.00

50.0000

 

◆特点

● 无需称量、溶解

● 已通过支原体实验

● 已进行0.2μm过滤灭菌

● 根据OECD Guideline No.201组成

 


◆OECD培养基的配制方法


通过将OECD培养基、浓缩液Ⅰ~Ⅳ混合、稀释,根据OECD TEST GUIDELINE No.201进行OECD培养基的配制。

配置1L的OECD培养基时

 

产品名称

需要量

OECD培养基, 浓缩液Ⅰ(×100)

10 mL

OECD培养基, 浓缩液Ⅱ(×1,000)

 1 mL

OECD培养基, 浓缩液Ⅲ(×1,000)

 1 mL

OECD培养基, 浓缩液Ⅳ(×1,000)

 1 mL

混合各浓缩液的需要量,稀释至1L

 

产品编号

产品名称

级别/厂家

规格

162-03652

Potassium Dichromate

试药特级

 25 g

196-15645

Sterile Water, Endotoxin Free

细胞培养

 500 mL

316-90101

Distilled Water, Deionized, Sterile

Nippongene

 100 mL

312-90103

100 mL×6

318-90105

 500 mL

012-11872

Agar (Powder)

植物培养

 25 g

016-11875

500 g

 

农残前处理柱(短柱)


产品编号 产品名称 产品规格 产品等级 产品价格
296-32651 Presep® -C Agri (Short)
农残前处理柱(短柱)
10 pcs×5 for Sample Pretreatment

农残前处理柱(短柱)

农残前处理柱(短柱)

Presep® -C Agri (Short)

  该固相萃取柱使用凝胶树脂(Styrene Divinylbenzene-Methacrylate)作为吸附材料,可迅速简便地进行农药残留分析的前处理。



◆特点


1、亲水性及疏水性聚合物凝胶可吸附水中存在的微量疏水性成分。

2、对难以回收的高极性及金属配位成分(Asulam•Oxine copper等)也具有极高的回收率。

3、使用柱处理器spe(减压浓缩装置),可在50分钟以内同时处理24个样本。还可使用

   Presep®-C Agri (Short),利用加压式定流泵进行回收、浓缩处理。

4、进行HPLC分析时,与Wakopak® WS Agri-9及专用洗提液组合使用,可迅速简便定量分析多种

   符合日本公定法规定的农药。

5、进行GC分析时,使用SGE公司的毛细管色谱柱,可进行选择性更高的分析。

Presep®-C Agri(Short)的回收率探讨(GC-MS分析条件)

固相萃取条件


色谱柱:Presep® -C Agri(Short)

柱活化:二氯甲烷5Ml,甲醇5mL,蒸馏水5mL

水样:在200mL蒸馏水中添加标准液100uL

固相吸附:以10-20mL/min的流速向柱进样

脱水:对柱通气10分钟

洗脱、脱水:将两个Presep®-C Na2SO4萃取柱连接至固相柱前端,用2.5mL二氯甲烷洗脱。

      然后拆下Presep®-C Agri(Short)后,用6mL二氯甲烷清洗Na2SO4柱,再与洗脱液混合。

浓缩:加入2mL己烷,吹氮浓缩至0.8mL (不得吹干)

分析样品:加入内标液100uL,用己烷定容至1mL(在分析结束前需冷藏保存)

标准液:10ug/mL丙酮溶液

内标液:萘嵌戊烷,苯并菲,2ug/mL己烷溶液为调整GC/MS分析中得灵敏度变化而添加

    两个Presep® -C Na2SO4脱水柱连接好后,用10mL二氯甲烷清洗后再使用。

 


GC分析条件


GC:HP5890 PACKARD SERIESE

GC/MS:AUTO mass JMS-AM150型(JEOL)

Column:100%Dimethyl Polysiloxane,0.25mm*30m,膜厚0.25um

Injector:250℃

升温:60℃(2min)-120℃(20℃/min)-300℃(10℃/min)-300℃(5min)

进样量:2.0uL

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒 D-Mannose/D-Fructose/D-Glucose Assay kit 货号:K-MANGL Megazyme中文站

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒

英文名:D-Mannose/D-Fructose/D-Glucose Assay kit

货号:K-MANGL

规格:55 assays per kit

The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.

UV-method for the determination of D-Mannose, D-Fructose
and D-Glucosein foodstuffs, yeast cell preparations and
other materials

Principle:
(hexokinase)
(1) D-Mannose / D-fructose / D-glucose + ATP →
M-6-P / F-6-P / G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphomannose isomerase) (phosphoglucose isomerase)
(3) M-6-P ↔ F-6-P ↔ G-6-P

Kit size: 55 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 0.7 mg/L
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other
materials (e.g. biological cultures, samples, etc.)
Method recognition: Novel method

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

 Q1. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q5. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q6. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q7. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q8. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q9. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q10. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q11. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q12. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

肌环己六氢醇脱氢酶(枯草芽孢杆菌) myo-Inositol dehydrogenase (Bacillus subtilis) 货号:E-INDHBS Megazyme中文站

肌环己六氢醇脱氢酶(枯草芽孢杆菌)

英文名:myo-Inositol dehydrogenase (Bacillus subtilis)

货号:E-INDHBS

规格:500 Units

High purity recombinant myo-Inositol dehydrogenase (B. subtilis) for use in research, biochemical enzyme assays and 
in vitro diagnostic analysis. 

EC 1.1.1.18

Inositol 2-dehydrogenase; myo-Inositol : NAD+ 2-oxidoreductase.

Recombinant from B. subtilis. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 50 U/mg (25oC, pH 9.6, myo-inositol). 

Stable at 4oC for > 2 years. 

暂无问题解答

暂无视频

乙酸[AF法]检测试剂盒 Acetic Acid (ACS; analyser format) 货号:K-ACETAF Megazyme中文站

乙酸[AF法]检测试剂盒

英文名:Acetic Acid (ACS; analyser format)

货号:K-ACETAF

规格:141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL)

(乙酰-CoA合成酶)(1)(柠檬酸合成酶)(2)2(L-苹果酸脱氢酶)+170.5 mL 配好的试剂 (R1 + R2)分光光度计,340 nm~15min10mg/L(使用推荐方法)葡萄酒、啤酒、水果和果汁、软饮料、醋、蔬菜、泡菜乳制品(如奶酪)、肉、鱼、面包、焙烤食品(和发酵粉)、番茄酱、酱油、蛋黄酱、调味汁、纸(硬纸板)、茶、医药品(如注射液)、饲料和其他原料(生物培养基、样品等)该实验方法已通过EN、ISO、ICUMSA、德国和荷兰的认证           

优点:

不会浪费ACS溶液(提供的  为稳定的悬浮液)加入PVP防止丹宁酸的抑制自动分析检测时配置好的试剂非常稳定    (> 5天, 4摄氏度)

最终反应液中的乙酸线性高达已通过法国葡萄酒大学验证

价格低廉     (每ml试剂的成本)所有试剂配制后的稳定性      >2年

 

Analyser format for the specific assay of acetic acid (acetate) in beverages and food products. Content:141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL)

Analyser format UV-method for the determination of Acetic Acid 
in foodstuffs, beverages and other materials

Principle:
                           (acetyl-CoA synthetase)
(1) Acetic acid + ATP + CoA → acetyl-CoA + AMP + pyrophosphate

                                                 (citrate synthase)
(2) Acetyl-CoA + oxaloacetate + H2O → citrate + CoA

                (L-malate dehydrogenase)
(3) L-Malate + NAD+ ↔ oxaloacetate + NADH + H+

Kit size:                           141.6 mL of prepared reagent (R1 + R2) 
Method:                           Spectrophotometric at 340 nm
Reaction time:                 ~ 15 min
Detection limit:                10 mg/L (recommended assay format)
Application examples: 
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, 
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products 
(and baking agents), ketchup, soy sauce, mayonnaise, dressings, 
paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), 
feed and other materials (e.g. biological cultures, samples, etc.) 
Method recognition:     
Methods based on this principle have been accepted by EN, ISO, 
ICUMSA, IFU and MEBAK

 

Advantages

  • No wasted ACS solution (stable suspension supplied)
     
  • PVP incorporated to prevent tannin inhibition
     
  • Very stable reagent when prepared for auto-analyser applications (> 3 days at 4°C)
     
  • Linear calibration up to 30 μg/mL of acetic acid in final reaction solution
     
  • Validated by the University of Wine, Suze la Rousse, France
     
  • Very competitive price (cost per mL of reagent)
     
  • All reagents stable for > 2 years after preparation

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and   therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. What are the major the differences between the various acetic acid test kits?

Megazyme produces 4 acetic acid test kits:
K-ACET: uses the traditional ACS reaction.  Manual format for use with spectrophotometers.
K-ACETAF: uses the traditional ACS reaction.  Automated format for use with auto-analysers.
K-ACETAK: uses the more recently developed and more rapid acetate kinase reaction.  Automated format for use with auto-analysers.
K-ACETRM: uses the more recently developed and more rapid acetate kinase reaction.  Manual format for use with spectrophotometers. 

Q5. Does the decolourising preparation remove some VA during the process?

No, however the sample preparation process can be tested by adding a known amount of acetic acid standard and assessing the recovery of this. 

Q6. Can acetic acid be measured in culture/fermentation media?

Acetic acid in liquid cell culture media/supernatants or fermentation samples can be determined without any sample treatment (except clarification by centrifugation or filtration) and appropriate dilution in distilled water. 

Q7. Which acetic acid kit is recommended for a 96-well microplate format?

Auto-analysers use ~ 0.315 mL reaction volumes and pathlengths between 4-8 mm which is similar to a standard 96-well microplate where a 0.315 mL reaction volume would give a pathlength of ~ 6-7 mm.  Therefore K-ACETAK or K-ACETAF can be used directly in a 96-well microplate format with minimal assay optimisation.
If preferred, K-ACET or K-ACETRM may also be easily converted for use in a 96-well microplate format.  Basically, the assay volumes for the cuvette format must be reduced approximately 10-fold for use in a 96-well microplate.  However, some assay optimisation may be required (e.g. increased enzyme concentration etc.) and unlike the cuvette which has a set pathlength of 1 cm, the pathlength in the microplate is dependent upon the volume of liquid in the well.  Therefore to enable the calculation of the amount of analyte in the samples from tests performed in the microplate format one of the following must be done:

  1. The easiest method is to use a microplate reader that has a pathlength conversion capability (i.e. the microplate reader can detect the pathlength of each well and convert the individual readings to a 1 cm pathlength).  This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm pathlength) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values.


Acetic Acid Kit Recommendation For Microplate Format:
Either K-ACETRM or K-ACETAK is recommended for use in a 96-well microplate format and the main advantages/disadvantages are described below:
K-ACETRM:
The assay volumes of this kit should be reduced by 10-fold for use in a 96-well microplate format (some assay optimisation may be required, e.g. increased enzyme concentration etc.).
The calculation of results is achieved as outlined above in either of points 1, 2 or 3. 

Q8. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q9. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q10. Is the acetic acid kit specific for acetate?

Ethyl acetate, butyrate and propionate may react more slowly than acetate. Free fatty acids are not measured.

Q11. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q12. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q13. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q14. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

视频

土壤DNA提取试剂盒


产品编号 产品名称 产品规格 产品等级 产品价格
319-06201 ISOIL for Beads Beating
土壤DNA提取试剂盒
50次

土壤DNA提取试剂盒

ISOIL for Beads Beating

 


原理

  本品为土壤DNA提取试剂盒。利用珠状物的强物理性粉碎及试剂的溶菌作用,从具有坚固细胞壁的微生物中提取DNA。因此,可用于PCR-DGGE等分析上土壤微生物的群集构造,利用定量土壤DNA推算土壤生物量。

  土壤等环境中存在各种各样的微生物。但其中99%以上为不能培养,或极难培养的微生物。依赖培养法分析土壤微生物具有一定的局限性。因此,像这类坏境样品一般采用不经培养,直接提取DNA进行分析的手法。近几年普遍盛行:利用土壤和污泥等环境样品直接提取出的DNA,进行基因复制及PCR-DGGE等分析微生物群集构造。

  海外制造商正在销售的集中土壤DNA提取试剂盒,都较难从黑土(占日本的国土的20%,耕地的50%)等火山灰土壤中提取DNA,无法与日本的土壤相融合。本试剂盒适用于提取日本土壤DNA,可短时间、高产率提取出高纯度DNA。

 

       本品采用在界面活性剂存在下加热提取的方法作为DNA提取法。利用Beads Beating(珠状粉碎),采用物理性菌体粉碎重组法。利用Beads Beating物理性截断,可从具有坚固细胞壁的微生物中,提取出反映实际土壤微生群集构造的土壤DNA。因此,本品提取出的土壤DNA,可用于PCR-DGGE等分析土壤微生物的群集构造和进行土壤诊断,利用定量土壤DNA推算土壤生物量等。DNA提取时间大约需要60分钟。

       另外,使用本品时,请不要使用其他用途的Beads Beating(珠状粉碎机)。

 

 

特征

 ● 可从火山灰土壤中提取DNA

 ● 可有效提取高纯度DNA

 ● 最短40分钟,即可提取出DNA

 ● 容易按比例增加

 ● 可高效提取DNA

 ● 适用于PCR-DGGE法及土壤DNA定量

 

 

内容

ISOL

 

Lysis Solution HE 50mL×1
Lysis Solution   20S 1.25mL×2
Purification Solution 20mL×1
Precipitation Solution 40mL×1
Wash Solution 50mL×1
Ethachinmate 100µL×1
TE(pH8.0) 5mL×1
poly-roto 1个
手册 1本
保存条件 室温

【梅特勒】电导率标准液 校正溶液—上海金畔生物

上海金畔生物科技有限公司提供 梅特勒 84uS/cm电导率标准液1瓶x250mL、校正溶液51302153 

 

型号(订货号) 说明

51343180 3M KCI溶液1瓶x25mL
51350072 3M KCI溶液1瓶x250mL
51343184 3M KCI溶液含AgCL饱和溶液1瓶x25mL
51350074 3M KCI溶液含AgCL饱和溶液1瓶x250mL
51343185 FriscoLyt-B电解液1瓶x25mL
51350076 FriscoLyt-B电解液1瓶x250mL
51350100 胃蛋白酶/盐酸清洗液1瓶x250mL
51350102 硫脲清洗液
51350104 电极活化液25mL
51350002 2.00pH缓冲液Technical Buffer 1瓶x250mL
51350004 4.01pH缓冲液Technical Buffer 1瓶x250mL
51350006 7.00pH缓冲液Technical Buffer 1瓶x250mL
51350008 9.21pH缓冲液Technical Buffer 1瓶x250mL
51350010 10.00pH缓冲液Technical Buffer 1瓶x250mL
51350012 11.00 pH缓冲液Technical Buffer 1瓶x250mL
51302069 4.01pH缓冲液30袋x20mL
51302047 7.00pH缓冲液30袋x20mL
51302070 9.21pH缓冲液30袋x20mL
51302068 4.01/7.00/9.21pH缓冲液各10袋x20mL
51302079 10.01pH缓冲液30袋x20mL
51350052 4.006pH缓冲液NIST/DIN Buffer 1瓶x250mL
51350054 6.865pH缓冲液NIST/DIN Buffer 1瓶x250mL
51350056 9.180pH缓冲液NIST/DIN Buffer 1瓶x250mL
51350058 10.012pH缓冲液NIST/DIN Buffer 1瓶x250mL
51350060 氧化还原缓冲液220mV, pH7(UH=427mV) 1瓶x250mL
51302153 84uS/cm电导仪标准液1x250mL
51350092 1413uS/cm电导仪标准液1瓶x250mL
51350094 12.88mS/cm电导仪标准液1瓶x250mL
51302049 1413uS/cm电导仪标准液30袋x20mL
51302050 12.88mS/cm电导仪标准液30袋x20mL

公司联系方式
更多产品,更多优惠,请联系我们!
上海金畔生物科技有限公司
地 址: 上海市浦东新区东靖路699弄36栋701室
邮 编: 201208
固话总机:
订货热线:15221999938
24小时短信服务:15221999938
服务投诉:15221999938
网 址: www.jinpanbio.com
公司博客:www.jinpanbio.cn
Email:sales@jinpanbio.co

硝基呋喃类分析标准品


产品编号 产品名称 产品规格 产品等级 产品价格
015-21171 1-Aminohydantoin Hydrochloride Standard
1-氨基海因盐酸盐标准品
200mg for High Performance Liquid Chromatography
014-25541 AMOZ Standard
硝基呋喃代谢物AMOZ标准品
100mg for High Performance Liquid Chromatography
011-25551 AOZ Standard
3-氨基-2-恶唑烷酮标准品
100mg for High Performance Liquid Chromatography
199-14591 Semicarbazide Hydrochloride Standard
盐酸氨基脲标准品
200mg for High Performance Liquid Chromatography
146-08511 Nitrofurazone Standard
呋喃西林标准品
200mg for High Performance Liquid Chromatography
142-08731 Nitrofurantoin Standard
呋喃妥因标准品
100mg for High Performance Liquid Chromatography
063-03651 Furazolidone Standard
呋喃唑酮标准品
200mg for High Performance Liquid Chromatography
062-05441 Furaltadone Hydrochloride Standard 100mg for High Performance Liquid Chromatography
291-33561 Presep® (Luer Lock) Diatomaceous Earth, Granular Type M(4.5g/25ml) 100EA for Sample Pretreatment
232-02661 Wakopak® Ultra C18-5 Φ4.6mm × 250mm (W) 1Column(W)

硝基呋喃类分析标准品


      (http://www.wako-chem.co.jp/siyaku/product/analysis/nitrofuran/index.htm)

      硝基呋喃类(呋喃西林,呋喃妥因,呋喃唑酮,呋喃它酮)是呋喃系合成抗菌剂,一般作为兽药用于治疗细菌

感染,但多个国家禁止其用于食用动物身上。

和光提供可用于分析硝基呋喃的各种标准品和分析柱、萃取用硅藻土色谱柱。

分析例子


硝基呋喃类分析标准品


※使用下述分析法检测被衍生体化的各种标准品的峰,名称缩写如下。

AMOZ:3 – 氨基-5 – 甲基-2 – 吗啉代 – 恶唑烷酮

AOZ:3 – 氨基-2 – 恶唑烷酮

AHD:1 – 氨基海

 


样品处理

各分析化合物

↓+0.1M HCl, 0.05M o-硝基苯甲醛/DMSO溶液

衍生物化(37℃,16hr)

↓+0.1M K2HPO4,1M NaOH

调整成pH7~8

进样到多孔性硅藻土萃取柱

↓+乙酸乙酯

洗脱

减压浓缩

↓+CH3CN:H2O=1:1

过滤(样品)

 

<分析条件>

进样量:样品5μl

使用色谱柱:Wakopak Ultra C18-5 4.6mm×250mm

洗脱液:A;0.1vol%醋酸溶液 B;乙腈



色谱柱温度:40℃

检测机器:Shimadzu SPD-M10Avp(UV260nm)

样品以日本厚生劳动省公告实验法的“呋喃妥因、呋喃它酮和呋喃唑酮试验方法”方法调整。



时间(分)

B(%)

0-20

20-80

20-30

80

◆相关产品

产品编号

产品名称

规格

包装

223-02053
227-02051

兽药混合标准液(喹诺酮类药物)(各20μg/ml)

高速液相色谱

1ml x 5A
     1ml

224-02083
228-02081

兽药混合标准液(磺胺类+叶酸代谢拮抗剂)(各20μg/ml)

高速液相色谱

1ml x 5A
     1ml

221-02093
225-02091

兽药混合标准液(大环内酯类)(各20μg/ml乙腈溶液)

高速液相色谱

1ml x 5A
     1ml

224-02103
228-02101

兽药混合标准液(着色剂)(各20μg/ml甲醇溶液)

高速液相色谱

1ml x 5A
     1ml

224-02201
 220-02203

兽药混合标准液(显影剂)(各20μg/ml乙腈溶液)

高速液相色谱

1ml x 5A
     1ml



PL系列兽药混合标准液

产品编号

产品名称

规格

包装

220-01681
226-01683

兽药混合标准液PL-1-3(各20μg/ml甲醇溶液)

高速液相色谱

1ml x 5A
     1ml

227-01691
223-01693

兽药混合标准液PL-2-1(各20μg/ml甲醇溶液)

高速液相色谱

1ml x 5A
     1ml

PFA平底容器


产品编号 产品名称 产品规格 产品等级 产品价格
WEB15274 PFA平底容器7mL (0225) 7mL
WEB15275 PFA平底容器15mL (0025) 15mL
WEB15277 PFA平底容器23mL (0275) 23mL
WEB15278 PFA平底容器33mL (0201) 33mL
WEB15279 PFA平底容器60mL (0202) 60mL
WEB15280 PFA平底容器60mL (0102) 60mL
WEB15281 PFA平底容器90mL (0103) 90mL
WEB15282 PFA平底容器120mL (0104) 120mL
WEB15283 PFA平底容器180mL (0103L) 180mL
WEB15284 PFA平底容器180mL (0106) 180mL
WEB15285 PFA平底容器240mL (0108) 240mL
WEB15286 PFA平底容器300mL (0110) 300mL
WEB15289 PFA平底容器500mL (0500) 500mL
WEB15290 PFA平底容器1L (1000) 1L

PFA平底容器

不含金属 样本储存容器!

PFA平底容器

氟树脂制(特氟龙),具有优良的化学稳定性,不含金属的平底样本容器。耐热性・耐寒性优异,使用温度-196℃~250℃。

◆特点

通过注塑成型生产
有重量感,坚固耐用。
内底是平的,具备优良的热传导率。
PFA材质,具有优良的化学稳定性,极少溶出金属离子。
耐热性・耐寒性优异,可在冷冻库内冷冻或通过油浴等方式加热;使用温度范围广(-196~250℃),在同一容器内允许骤冷骤热,或冷热交替操作。
高润滑不粘性,适用于处理高粘度样本或要求良好的可剥离性操作。

◆应用

容量小的可以作为无金属容器用于水质检查或用作ICP分析用样本储存容器。
中等容量的可作为化学液体反应容器或各种储存容器;大容量的可作为反应容器或强酸·有机溶剂等的废弃液体储存容器。

◆注意

0102·0103·0103L的盖子形状与照片不同。见下图:


PFA平底容器