Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents
Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

Products > Custom Shop Crystallization Reagents > SaltRx1 • SaltRx2 > Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

Applications

  • Individual reagents from SaltRx 1, SaltRx 2, and SaltRx HT for screening, optimization and production

Features

  • 185 ml volume
  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

HR2-907-** and HR2-909-**
** Refers to the reagent number in the kit.
For example, SaltRx 1 reagent number 1 = SaltRx HT reagent A1 = HR2-907-01
For example, SaltRx 2 reagent number 1 = SaltRx HT reagent E1 = HR2-909-01



Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

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Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

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Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

CAT NO

HR2-907-01

NAME

SaltRx 1 #01 / SaltRx HT A1 185ml

DESCRIPTION

1.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-02

NAME

SaltRx 1 #02 / SaltRx HT A2 185ml

DESCRIPTION

2.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-03

NAME

SaltRx 1 #03 / SaltRx HT A3 185ml

DESCRIPTION

1.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-04

NAME

SaltRx 1 #04 / SaltRx HT A4 185ml

DESCRIPTION

1.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-05

NAME

SaltRx 1 #05 / SaltRx HT A5 185ml

DESCRIPTION

1.5 M Ammonium chloride, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-06

NAME

SaltRx 1 #06 / SaltRx HT A6 185ml

DESCRIPTION

3.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-07

NAME

SaltRx 1 #07 / SaltRx HT A7 185ml

DESCRIPTION

3.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-08

NAME

SaltRx 1 #08 / SaltRx HT A8 185ml

DESCRIPTION

3.5 M Ammonium chloride, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-09

NAME

SaltRx 1 #09 / SaltRx HT A9 185ml

DESCRIPTION

2.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-10

NAME

SaltRx 1 #10 / SaltRx HT A10 185ml

DESCRIPTION

2.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-11

NAME

SaltRx 1 #11 / SaltRx HT A11 185ml

DESCRIPTION

2.2 M Sodium chloride, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-12

NAME

SaltRx 1 #12 / SaltRx HT A12 185ml

DESCRIPTION

3.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-13

NAME

SaltRx 1 #13 / SaltRx HT B1 185ml

DESCRIPTION

3.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-14

NAME

SaltRx 1 #14 / SaltRx HT B2 185ml

DESCRIPTION

3.2 M Sodium chloride, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-15

NAME

SaltRx 1 #15 / SaltRx HT B3 185ml

DESCRIPTION

1.0 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-16

NAME

SaltRx 1 #16 / SaltRx HT B4 185ml

DESCRIPTION

1.8 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-17

NAME

SaltRx 1 #17 / SaltRx HT B5 185ml

DESCRIPTION

1.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-18

NAME

SaltRx 1 #18 / SaltRx HT B6 185ml

DESCRIPTION

2.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-19

NAME

SaltRx 1 #19 / SaltRx HT B7 185ml

DESCRIPTION

0.7 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-20

NAME

SaltRx 1 #20 / SaltRx HT B8 185ml

DESCRIPTION

0.7 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-21

NAME

SaltRx 1 #21 / SaltRx HT B9 185ml

DESCRIPTION

1.2 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-22

NAME

SaltRx 1 #22 / SaltRx HT B10 185ml

DESCRIPTION

1.2 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-23

NAME

SaltRx 1 #23 / SaltRx HT B11 185ml

DESCRIPTION

0.4 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-24

NAME

SaltRx 1 #24 / SaltRx HT B12 185ml

DESCRIPTION

0.4 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-25

NAME

SaltRx 1 #25 / SaltRx HT C1 185ml

DESCRIPTION

0.4 M Magnesium formate dihydrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-26

NAME

SaltRx 1 #26 / SaltRx HT C2 185ml

DESCRIPTION

0.7 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-27

NAME

SaltRx 1 #27 / SaltRx HT C3 185ml

DESCRIPTION

2.0 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-28

NAME

SaltRx 1 #28 / SaltRx HT C4 185ml

DESCRIPTION

2.0 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-29

NAME

SaltRx 1 #29 / SaltRx HT C5 185ml

DESCRIPTION

2.0 M Sodium formate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-30

NAME

SaltRx 1 #30 / SaltRx HT C6 185ml

DESCRIPTION

3.5 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-31

NAME

SaltRx 1 #31 / SaltRx HT C7 185ml

DESCRIPTION

3.5 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-32

NAME

SaltRx 1 #32 / SaltRx HT C8 185ml

DESCRIPTION

3.5 M Sodium formate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-33

NAME

SaltRx 1 #33 / SaltRx HT C9 185ml

DESCRIPTION

1.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-34

NAME

SaltRx 1 #34 / SaltRx HT C10 185ml

DESCRIPTION

2.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-35

NAME

SaltRx 1 #35 / SaltRx HT C11 185ml

DESCRIPTION

1.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-36

NAME

SaltRx 1 #36 / SaltRx HT C12 185ml

DESCRIPTION

2.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-37

NAME

SaltRx 1 #37 / SaltRx HT D1 185ml

DESCRIPTION

2.5 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-38

NAME

SaltRx 1 #38 / SaltRx HT D2 185ml

DESCRIPTION

2.5 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-39

NAME

SaltRx 1 #39 / SaltRx HT D3 185ml

DESCRIPTION

2.5 M Ammonium nitrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-40

NAME

SaltRx 1 #40 / SaltRx HT D4 185ml

DESCRIPTION

6.0 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-41

NAME

SaltRx 1 #41 / SaltRx HT D5 185ml

DESCRIPTION

6.0 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-42

NAME

SaltRx 1 #42 / SaltRx HT D6 185ml

DESCRIPTION

6.0 M Ammonium nitrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-43

NAME

SaltRx 1 #43 / SaltRx HT D7 185ml

DESCRIPTION

1.5 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-44

NAME

SaltRx 1 #44 / SaltRx HT D8 185ml

DESCRIPTION

1.5 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-45

NAME

SaltRx 1 #45 / SaltRx HT D9 185ml

DESCRIPTION

1.5 M Sodium nitrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-907-46

NAME

SaltRx 1 #46 / SaltRx HT D10 185ml

DESCRIPTION

4.0 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-907-47

NAME

SaltRx 1 #47 / SaltRx HT D11 185ml

DESCRIPTION

4.0 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-907-48

NAME

SaltRx 1 #48 / SaltRx HT D12 185ml

DESCRIPTION

4.0 M Sodium nitrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-01

NAME

SaltRx 2 #01 / SaltRx HT E1 185ml

DESCRIPTION

1.0 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-02

NAME

SaltRx 2 #02 / SaltRx HT E2 185ml

DESCRIPTION

1.8 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-03

NAME

SaltRx 2 #03 / SaltRx HT E3 185ml

DESCRIPTION

1.5 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-04

NAME

SaltRx 2 #04 / SaltRx HT E4 185ml

DESCRIPTION

2.4 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-05

NAME

SaltRx 2 #05 / SaltRx HT E5 185ml

DESCRIPTION

1.0 M Sodium phosphate monobasic monohydrate, Potassium phophate dibasic / pH 5.0 (0.98 M Sodium phosphate monobasic monohydrate, 0.02 M Potassium phosphate dibasic)

PRICE

$187.00

cart quote

CAT NO

HR2-909-06

NAME

SaltRx 2 #06 / SaltRx HT E6 185ml

DESCRIPTION

1.0 M Sodium phosphate monobasic monohydrate, Potassium phophate dibasic / pH 6.9 (0.35 M Sodium phosphate monobasic monohydrate, 0.65 M Potassium phosphate dibasic)

PRICE

$187.00

cart quote

CAT NO

HR2-909-07

NAME

SaltRx 2 #07 / SaltRx HT E7 185ml

DESCRIPTION

1.0 M Sodium phosphate monobasic monohydrate, Potassium phophate dibasic / pH 8.2 (0.04 M Sodium phosphate monobasic monohydrate, 0.96 M Potassium phosphate dibasic)

PRICE

$187.00

cart quote

CAT NO

HR2-909-08

NAME

SaltRx 2 #08 / SaltRx HT E8 185ml

DESCRIPTION

1.8 M Sodium phosphate monobasic monohydrate, Potassium phophate dibasic / pH 5.0 (1.764 M Sodium phosphate monobasic monohydrate, 0.036 M Potassium phosphate dibasic)

PRICE

$187.00

cart quote

CAT NO

HR2-909-09

NAME

SaltRx 2 #09 / SaltRx HT E9 185ml

DESCRIPTION

1.8 M Sodium phosphate monobasic monohydrate, Potassium phophate dibasic / pH 6.9 (0.63 M Sodium phosphate monobasic monohydrate, 1.17 M Potassium phosphate dibasic)

PRICE

$187.00

cart quote

CAT NO

HR2-909-10

NAME

SaltRx 2 #10 / SaltRx HT E10 185ml

DESCRIPTION

1.8 M Sodium phosphate monobasic monohydrate, Potassium phophate dibasic / pH 8.2 (0.072 M Sodium phosphate monobasic monohydrate, 1.728 M Potassium phosphate dibasic)

PRICE

$187.00

cart quote

CAT NO

HR2-909-11

NAME

SaltRx 2 #11 / SaltRx HT E11 185ml

DESCRIPTION

0.5 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-12

NAME

SaltRx 2 #12 / SaltRx HT E12 185ml

DESCRIPTION

1.0 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-13

NAME

SaltRx 2 #13 / SaltRx HT F1 185ml

DESCRIPTION

1.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-14

NAME

SaltRx 2 #14 / SaltRx HT F2 185ml

DESCRIPTION

1.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-15

NAME

SaltRx 2 #15 / SaltRx HT F3 185ml

DESCRIPTION

1.5 M Ammonium sulfate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-16

NAME

SaltRx 2 #16 / SaltRx HT F4 185ml

DESCRIPTION

2.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-17

NAME

SaltRx 2 #17 / SaltRx HT F5 185ml

DESCRIPTION

2.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-18

NAME

SaltRx 2 #18 / SaltRx HT F6 185ml

DESCRIPTION

2.5 M Ammonium sulfate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-19

NAME

SaltRx 2 #19 / SaltRx HT F7 185ml

DESCRIPTION

0.8 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-20

NAME

SaltRx 2 #20 / SaltRx HT F8 185ml

DESCRIPTION

0.8 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-21

NAME

SaltRx 2 #21 / SaltRx HT F9 185ml

DESCRIPTION

0.8 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-22

NAME

SaltRx 2 #22 / SaltRx HT F10 185ml

DESCRIPTION

1.5 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-23

NAME

SaltRx 2 #23 / SaltRx HT F11 185ml

DESCRIPTION

1.5 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-24

NAME

SaltRx 2 #24 / SaltRx HT F12 185ml

DESCRIPTION

1.5 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-25

NAME

SaltRx 2 #25 / SaltRx HT G1 185ml

DESCRIPTION

1.0 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-26

NAME

SaltRx 2 #26 / SaltRx HT G2 185ml

DESCRIPTION

1.0 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-27

NAME

SaltRx 2 #27 / SaltRx HT G3 185ml

DESCRIPTION

1.0 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-28

NAME

SaltRx 2 #28 / SaltRx HT G4 185ml

DESCRIPTION

1.8 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-29

NAME

SaltRx 2 #29 / SaltRx HT G5 185ml

DESCRIPTION

1.8 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-30

NAME

SaltRx 2 #30 / SaltRx HT G6 185ml

DESCRIPTION

1.8 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-31

NAME

SaltRx 2 #31 / SaltRx HT G7 185ml

DESCRIPTION

0.7 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-32

NAME

SaltRx 2 #32 / SaltRx HT G8 185ml

DESCRIPTION

0.7 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-33

NAME

SaltRx 2 #33 / SaltRx HT G9 185ml

DESCRIPTION

0.7 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-34

NAME

SaltRx 2 #34 / SaltRx HT G10 185ml

DESCRIPTION

1.0 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-35

NAME

SaltRx 2 #35 / SaltRx HT G11 185ml

DESCRIPTION

1.3 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-36

NAME

SaltRx 2 #36 / SaltRx HT G12 185ml

DESCRIPTION

1.4 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-37

NAME

SaltRx 2 #37 / SaltRx HT H1 185ml

DESCRIPTION

0.6 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-38

NAME

SaltRx 2 #38 / SaltRx HT H2 185ml

DESCRIPTION

1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-39

NAME

SaltRx 2 #39 / SaltRx HT H3 185ml

DESCRIPTION

0.6 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-40

NAME

SaltRx 2 #40 / SaltRx HT H4 185ml

DESCRIPTION

1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-41

NAME

SaltRx 2 #41 / SaltRx HT H5 185ml

DESCRIPTION

0.5 M Potassium thiocyanate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-42

NAME

SaltRx 2 #42 / SaltRx HT H6 185ml

DESCRIPTION

0.5 M Potassium thiocyanate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-43

NAME

SaltRx 2 #43 / SaltRx HT H7 185ml

DESCRIPTION

0.5 M Potassium thiocyanate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-44

NAME

SaltRx 2 #44 / SaltRx HT H8 185ml

DESCRIPTION

4.0 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6

PRICE

$187.00

cart quote

CAT NO

HR2-909-45

NAME

SaltRx 2 #45 / SaltRx HT H9 185ml

DESCRIPTION

4.0 M Ammonium acetate, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-46

NAME

SaltRx 2 #46 / SaltRx HT H10 185ml

DESCRIPTION

4.0 M Ammonium acetate, 0.1 M Tris pH 8.5

PRICE

$187.00

cart quote

CAT NO

HR2-909-47

NAME

SaltRx 2 #47 / SaltRx HT H11 185ml

DESCRIPTION

35% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

CAT NO

HR2-909-48

NAME

SaltRx 2 #48 / SaltRx HT H12 185ml

DESCRIPTION

60% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

PRICE

$187.00

cart quote

Support Material(s)

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents HR2-907-** Individual SaltRx 1 Reagents SDSIndividual SaltRx1 • SaltRx2 • SaltRx HT Reagents HR2-909-** Individual SaltRx 2 Reagents SDS

Related Item(S)

  • SaltRx 1 • SaltRx 2 • SaltRx HT

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

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Products > Optimization Screens > Additive Screen > Additive Screen • Additive Screen HT

Additive Screen • Additive Screen HT

Applications

  • Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility

Features

  • 18 classes of reagents
  • Highly concentrated (10x) reagent formulation
  • 96 unique reagents and excipients
  • Tube or Deep Well block format
  • Crystallization or sample solubility optimization

Description

Additive Screen is a library of reagents that can affect the solubility and crystallization of biological macromolecules, including both soluble and membrane proteins.

These reagents can perturb and manipulate sample-sample and sample-solvent interactions, as well as perturb water structure which can alter and improve both the solubility and crystallization of a sample. Additives can stabilize or engender conformity by specific interaction with the macromolecules. There are numerous reports of the use of additives to improve the quality and size of macromolecular crystals.1-5

Additive screen reagent classifications include multivalent, salt, amino acid, dissociating agent, linker, polyamine, osmolyte, chaotrope, co-factor, reducing agent, chelating agent, polymer, carbohydrate, non-detergent, amphiphile, detergent, non-volatile organic and volatile organic.

Additive Screen contains 96 unique reagents, 1 ml each.

Additive Screen HT contains 96 unique reagents, 1 ml each, in a single Deep Well block.

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Additive Screen • Additive Screen HT

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Additive Screen • Additive Screen HT
Additive Screen • Additive Screen HT
Additive Screen • Additive Screen HT

Additive Screen • Additive Screen HT

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Additive Screen • Additive Screen HT
Additive Screen • Additive Screen HT
Additive Screen • Additive Screen HT

CAT NO

HR2-428

NAME

Additive Screen

DESCRIPTION

1 ml, tube format

PRICE

$495.00

cart quote

CAT NO

HR2-138

NAME

Additive Screen HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$495.00

cart quote

Support Material(s)

Additive Screen • Additive Screen HT HR2-428 Additive Screen DocumentsAdditive Screen • Additive Screen HT HR2-428 Additive Screen SDSAdditive Screen • Additive Screen HT HR2-138 Additive Screen HT DocumentsAdditive Screen • Additive Screen HT HR2-138 Additive Screen HT SDSAdditive Screen • Additive Screen HT Additive Screen Formulation & Scoring DataAdditive Screen • Additive Screen HT Additive Screen Frequently Asked Questions

Related Item(S)

  • Individual Additive Screen Reagents

References

1. Crystallization of membrane proteins., Edited by Hartmut Michel, CRC Press (1991).

2. Crystallization of Nucleic Acids and Proteins: A Practical Approach., Edited by A. Ducruix and R. Giege, Oxford University Press (1992).

3. Cudney, R., et al., Screening and optimization strategies for macromolecular crystal growth., Acta Cryst. (1994) D50, 414-423.

4. Sousa R., Use of glycerol, polyols and other protein structure stabilizing agents in protein crystallization., Acta Cryst. (1995) D51, 271-277.

5. Trakhanov, S. and Quiocho, F.A., Influence of divalent cations on protein crystallization., Protein Science (1995) 4, 9, 1914-1919.

6. Crystallization and preliminary X-ray diffraction analysis of YhbJ from Escherichia coli, a key protein involved in the GlmYZ sRNA regulatory cascade. M. Resch, Y. Gopel, B. Gorke and R. Ficner. M. Resch, Y. Gopel, B. Gorke and R. Ficner.

7. Crystal-contact engineering to obtain a crystal form of the Kelch domain of human Keap1 suitable for ligand-soaking experiments. Stefan Horer, Dirk Reinert, Katja Ostmann, Yvette Hoevels and Herbert Nar. Acta Cryst. (2013). F69, 592-596.

8. Thermofluor-based optimization strategy for the stabilization and crystallization of Campylobacter jejuni desulforubrerythrin. Santos SP, Bandeiras TM, Pinto AF, Teixeira M, Carrondo MA, Romão CV. Protein Expr Purif. 2012 Feb;81(2):193-200. doi: 10.1016/j.pep.2011.10.001. Epub 2011 Oct 24. (Thermofluor using the Additive Screen)

Additive Screen • Additive Screen HT Additive Screen • Additive Screen HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Crystal Screen • Crystal Screen 2 • Crystal Screen HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Products > Crystallization Screens > Crystal Screen • Crystal Screen 2 > Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Applications

  • Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules
  • Sparse matrix additive screen

Features

  • The original sparse matrix screen
  • Sparse matrix formula efficiently samples salts, polymers, organics, & pH
  • Proven effective with more than 1,000 biological macromolecules
  • Tube or Deep Well block format

Description

The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.

A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth. 

Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.

Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.

Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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Crystal Screen • Crystal Screen 2 • Crystal Screen HT

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Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

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Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT

CAT NO

HR2-110

NAME

Crystal Screen

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-112

NAME

Crystal Screen 2

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-130

NAME

Crystal Screen HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

Crystal Screen • Crystal Screen 2 • Crystal Screen HT HR2-110 Crystal Screen DocumentsCrystal Screen • Crystal Screen 2 • Crystal Screen HT HR2-110 Crystal Screen SDSCrystal Screen • Crystal Screen 2 • Crystal Screen HT HR2-112 Crystal Screen 2 DocumentsCrystal Screen • Crystal Screen 2 • Crystal Screen HT HR2-112 Crystal Screen 2 SDSCrystal Screen • Crystal Screen 2 • Crystal Screen HT HR2-130 Crystal Screen HT DocumentsCrystal Screen • Crystal Screen 2 • Crystal Screen HT HR2-130 Crystal Screen HT SDSCrystal Screen • Crystal Screen 2 • Crystal Screen HT Crystal Screen Formulation & Scoring Data

Related Item(S)

  • Individual Crystal Screen • Crystal Screen 2 • Crystal Screen HT Reagents

References

1. Jancarik, J. & Kim, S.H. J. Appl. Cryst. 24, 409-411, (1991).

2. Cudney, R., Patel, S., Weisgraber, K., Newhouse, Y., and McPherson, A., Acta Cryst. (1994) D50, 414-423.

3. Expression, purification, crystallization and preliminary X-ray analysis of two arginine-biosynthetic enzymes from Mycobacterium tuberculosis. F. Moradian, C. Garen, L. Cherney, M. Cherney and M. N. G. James. Acta Cryst. (2006). F62, 986-988.

4. The development and application of a method to quantify the quality of cryoprotectant solutions using standard area-detector X-ray images. McFerrin and Snell, J. Appl. Cryst. (2002). 35, 538-545.

5. Get phases from arsenic anomalous scattering: de novo SAD phasing of two protein structures crystallized in cacodylate buffer. Liu X, Zhang H, Wang XJ, Li LF, Su XD. PLoS One. 2011;6(9):e24227. Epub 2011 Sep 2.

6. Cloning, crystallization and preliminary X-ray diffraction analysis of an intact DNA methyltransferase of a type I restriction-modification enzyme from Vibrio vulnificus. L. Huynh Thi Yen, S.-Y. Park and J.-S. Kim. Acta Cryst. (2014). F70, 489-492 [ doi:10.1107/S2053230X14004543 ]

7. Crystallization and preliminary X-ray analysis of a novel type of lipolytic hydrolase from Bacillus licheniformis. H. Ju, R. Pandian, K. Kim, K. K. Kim and T. D. Kim. Acta Cryst. (2014). F70, 473-475 [ doi:10.1107/S2053230X14004142 ]

Crystal Screen • Crystal Screen 2 • Crystal Screen HT Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Crystal Screen Cryo • Crystal Screen 2 Cryo蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo

Crystal Screen Cryo • Crystal Screen 2 Cryo

Crystal Screen Cryo • Crystal Screen 2 Cryo

Crystal Screen Cryo • Crystal Screen 2 Cryo

Crystal Screen Cryo • Crystal Screen 2 Cryo

Products > Crystallization Screens > Crystal Screen Cryo • Crystal Screen 2 Cryo > Crystal Screen Cryo • Crystal Screen 2 Cryo

Crystal Screen Cryo • Crystal Screen 2 Cryo

Applications

  • Primary biased sparse matrix screen with cryo for proteins, soluble peptides, nucleic acids, & water soluble small molecules.

Features

  • Developed at Hampton Research
  • Cryo ready screening reagents for cryocrystallography
  • Sparse matrix formula efficiently samples salts, polymers, organics, & pH
  • Tube or Deep Well block format
  • Screen for cryo and crystallization conditions using only one screen

Description

Determining initial and optimal cryoprotectant concentration is often a process of trial and error. One must find suitable cryoprotectant concentrations which stabilize the crystal while at the same time combine with the crystallization reagent to form an amorphous glass.

The Crystal Screen Cryo kits removes the guess work from determining the preliminary glycerol concentration required to mix with the crystallization reagent to form an amorphous glass. Crystal Screen Cryo reagents are formulated with the appropriate amount of glycerol required to form an amorphous glass with each unique reagent composition. Crystals obtained in Crystal Screen Cryo kits will already have a suitable concentration of glycerol in the reagent. This avoids exhaustive empirical searches for preliminary cryoprotectant conditions. From the preliminary cryoprotectant conditions, cryoprotectant concentration can be optimized to minimize mosaic spread and maximize diffraction resolution. 

Crystal Screen Cryo kits are biased sparse matrices designed for rapid screening of crystallization conditions of biological macromolecules in the presence of glycerol, the most frequently utilized cryoprotectant. The Crystal Screen Cryo kits utilize the original Crystal Screen™ and Crystal Screen 2™ protocol optimized to include the appropriate concentration of glycerol required to form an amorphous glass at 100°K. The primary screen variables are salt, pH, precipitant (salts, polymers, volatile organics, and non-volatile organics), and cryoprotectant concentration. The screen is a straightforward, effective, and practical kit for determining preliminary crystallization conditions and provides a good starting point for finding suitable cryoprotectant conditions for macromolecular crystals grown in a wide range of reagents. Crystal Screen Cryo is also effective in determining the solubility of a macromolecule in a wide range of precipitants and pH.

Crystal Screen Cryo contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991 and refined for cryo by Garman in 1996.

Crystal Screen 2 Cryo contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994 and refined for cryo by Snell et al in 2002.

Crystal Screen Cryo HT contains 1 ml each of reagents 1-48 from Crystal Screen Cryo and 48 reagents from Crystal Screen 2 Cryo in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers.

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Crystal Screen Cryo • Crystal Screen 2 Cryo

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Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo

Crystal Screen Cryo • Crystal Screen 2 Cryo

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Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo
Crystal Screen Cryo • Crystal Screen 2 Cryo

CAT NO

HR2-122

NAME

Crystal Screen Cryo

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-121

NAME

Crystal Screen 2 Cryo

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-133

NAME

Crystal Screen Cryo HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

Crystal Screen Cryo • Crystal Screen 2 Cryo HR2-122 Crystal Screen Cryo DocumentsCrystal Screen Cryo • Crystal Screen 2 Cryo HR2-122 Crystal Screen Cryo SDSCrystal Screen Cryo • Crystal Screen 2 Cryo HR2-121 Crystal Screen 2 Cryo DocumentsCrystal Screen Cryo • Crystal Screen 2 Cryo HR2-121 Crystal Screen Cryo 2 SDSCrystal Screen Cryo • Crystal Screen 2 Cryo HR2-133 Crystal Screen Cryo HT DocumentsCrystal Screen Cryo • Crystal Screen 2 Cryo HR2-133 Crystal Screen Cryo HT SDSCrystal Screen Cryo • Crystal Screen 2 Cryo Crystal Screen Cryo kits Formulation & Scoring Data

Related Item(S)

  • Individual Crystal Screen Cryo • Crystal Screen 2 Cryo • Crystal Screen Cryo HT Reagents

References

1. Garman, E.F. and Mitchell, E.P., J. Appl. Cryst. (1996) 29, 584- 587.

2. Structure of the single-stranded DNA-binding protein from Streptomyces coelicolor. Stefanic et al, Acta Cryst. (2009) D65, 974-979.

3. The development and application of a method to quantify the quality of cryoprotectant solutions using standard area-detector X-ray images. McFerrin and Snell, J. Appl. Cryst. (2002). 35, 538-545.

4. Cudney, R., Patel, S., Weisgraber, K., Newhouse, Y., and McPherson, A., Acta Cryst. (1994) D50, 414-423.

Crystal Screen Cryo • Crystal Screen 2 Cryo Crystal Screen Cryo • Crystal Screen 2 Cryo

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Detergent Screen蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Detergent Screen
Detergent Screen

Detergent Screen

Detergent Screen

Detergent Screen

Detergent Screen

Products > Optimization Screens > Detergent Screen > Detergent Screen

Detergent Screen

Applications

  • Manipulate sample-sample and sample-solvent interactions, including non-specific aggregation due to hydrophobic interactions, to alter sample solubility, or obtain or improve crystalline samples.

Features

  • Developed at Hampton Research
  • For use with either membrane or soluble proteins
  • Compatible with vapor diffusion, microbatch, and free interface diffusion methods
  • 96 Deep Well block format
  • Classification of Detergents in screen
    • Ionic
    • Non-ionic
    • Zwitterionic
    • Non-detergent Sulfobetaines

Description

Note: HR2-407 Detergent Screen is a new formulation and has replaced the original HR2-406 Detergent Screen HT.

For crystallization to take place, a protein must be soluble in aqueous solution. Ideally the sample should be homogeneous, monodisperse, and in a state of aggregation conducive to interactions which will promote the nucleation and subsequent growth of a crystal. Unfavorable aggregation can compete with, obstruct, and prevent the normal ordering or the sample into a crystal. Many proteins, typically membrane proteins, membrane associated proteins, and soluble proteins contain hydrophobic residues on the surface which can lead to non-specific aggregation, a deterrent to solubility and crystallization. Mild, biological detergents can perturb and manipulate hydrophobic sample-sample and sample-solvent interactions. Incorporating detergents into the solubilization or crystallization reagent during screening and optimization is a popular and effective strategy for identifying conditions which promote and enhance solubility and crystallization. Besides improving the crystallization properties for some proteins and nucleic acids, detergents have also been shown to alleviate problems of twinning and secondary nucleation and can also produce different crystal forms.

Since it can be difficult to impossible a priori to predict and select which detergent or solubilizing agent will minimize the general nonspecific interactions yet at the same time sustain the specific interactions necessary for crystallization, the screening of a portfolio of detergent and solubilizing reagents is an efficient and effective strategy to identify the appropriate and best detergent reagent. The Detergent Screen is a set of solubilization and crystallization specific, mild, biological detergent reagents in a ready to use format at concentrations appropriate for solubility and crystallization screens. The Detergent Screen format allows one to identify a detergent effect as well as help select the best detergent reagent.

Each Detergent Screen kit contains 96 unique detergents. 0.25 ml of detergent solution is formulated at 10 times the reported CMC (unless otherwise noted) in sterile filtered deionized water. The Detergent Screen kit contains 96 solutions in a single Deep Well block, a single sheet of AlumaSeal II Sealing Film, User Guide, Formulations, and Scoring Sheet.

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Detergent Screen

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Detergent Screen
Detergent Screen
Detergent Screen

Detergent Screen

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Detergent Screen
Detergent Screen
Detergent Screen

CAT NO

HR2-407

NAME

Detergent Screen

DESCRIPTION

0.25 ml, Deep Well block format

PRICE

$495.00

cart quote

Support Material(s)

Detergent Screen HR2-407 Detergent Screen DocumentsDetergent Screen HR2-407 Detergent Screen SDSDetergent Screen Detergent Screen Formulation & Scoring Data

Related Item(S)

  • Individual Detergent Screen Reagents

References

1. Protein crystallogra phy with non detergent sulfobetaines. Laurent Vuillard, Bassem Baalbakia, Mogens Lehmanna, Sofie Norager, Pierre Legrand, Michel Roth. 1996, J Cryst Growth 168 150-154.

2. A defined protein–detergent–lipid complex for crystallization of integral membrane proteins: The cytochrome b6f complex of oxygenic photosynthesis. Huamin Zhang, Genji Kurisu, Janet L. Smith, and William A. Cramer. Proc Natl Acad Sci U S A. 2003 April 29, 100(9): 5160–5163.

3. An experiment regarding crystalliza tion of soluble proteins in the pres-ence of b-octyl-glucoside. McPherson A., Koszelak, S., Axelrod, H., Day, J., Williams, R., McGrath, M., Robinson, L., and Cascio, D. 1986, J. Biol. Chem. 261: 1969.

4. McPherson A. (1999). Crystallization of Biological Macromolecules. Cold Spring Harbor Laboratory Press, 253-258.

5. Crystallization of membrane proteins. Edited by Hartmut Michel, CRC Press, 1991.

6. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992 175-191.

7. The effects of neutral detergents on the crystallization of soluble proteins. A. McPherson et al. 1986, J. Crystal Growth, 76, 547-553.

8. Kuhlbrand, W., Quarterly Rev. Biophys., 21, 429, 1988.

9. Garavito, R.M., & Picot, D., Methods, A Companion to Methods in En-zymology, 1, 57, 1990.

10. The growth and characterization of membrane protein crystals. R. Mi-chael Garavito, Zora Markovic-Housley, John A. Jenkins. 1986, Journal of Crystal Growth, 76, 701-709.

11. Screening and optimization strategies for macromolecular crystal growth. R. Cudney, S. Patel, K. Weisgraber, Y. Newhouse and A. McPherson. 1994, Acta Cryst. D50, 414-423.

Detergent Screen Detergent Screen

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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GRAS Additive蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

GRAS Additive
GRAS Additive

GRAS Additive

GRAS Additive

GRAS Additive

GRAS Additive

Products > Optimization Screens > GRAS Additive > GRAS Additive

GRAS Additive

Applications

  • GRAS Additive™ is an optimization kit designed to evaluate 96 unique water soluble reagents and their ability to influence, promote and improve the crystallization of biological macromolecules.

Features

  • Developed at Hampton Research
  • Bio focused additive screen for the optimization of biological macromolecular crystals
  • For use with soluble proteins, membrane proteins, and biological therapeutics
  • Generally Recognized As Safe reagent formulation
  • Compatible with vapor diffusion, microbatch, free interface diffusion

Description

GRAS Additive is an optimization kit designed to allow rapid and convenient evaluation of 96 unique reagents and their ability to influence the crystallization of biological macromolecules, including but not limited to soluble proteins, membrane proteins, and biological therapeutics.1-5

The chemicals in GRAS Additive have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct.5, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

The 96 by 1 ml, deep well block reagent screen, is designed to be compatible with most popular crystallization reagents including reagents utilized in Hampton Research screens. Compatible with vapor diffusion, microbatch, and free interface diffusion. For research use only.

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GRAS Additive

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GRAS Additive
GRAS Additive
GRAS Additive

GRAS Additive

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GRAS Additive
GRAS Additive
GRAS Additive

CAT NO

HR2-459

NAME

GRAS Additive

DESCRIPTION

1 ml, Deep Well block format

PRICE

$545.00

cart quote

Support Material(s)

GRAS Additive HR2-459 GRAS Additive DocumentsGRAS Additive HR2-459 GRAS Additive SDSGRAS Additive GRAS Additive Formulation & Scoring Data

Related Item(S)

  • Individual GRAS Additive Reagents

References

1. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology 156 (2006) 387-406.

2. A novel strategy for the crystallization of proteins: X-ray diffraction validation. Steven B. Larson, John S. Day, Robert Cudney, and Alexander McPherson. Acta Cryst. (2007) D63, 310-318.

3. Development of an alternative approach to protein crystallization. McPherson, Alexander; Nguyen, Chieniang; Larson, Steven B; Day, John S; Cudney, Bob. J Struct Funct Genomics, Volume 8, Number 4, December 2007, 193-198.

4. Progress in the Development of an Alternative Approach to Macromolecular Crystallization. S. B. Larson,J. S. Day, C. Nguyen, R. Cudney, and A. McPherson. Crystal Growth & Design 2008 Volume 8, No. 8 3038-3052.

5. Wishart DS, Jewison T, Guo AC, Wilson M, Knox C, et al., HMDB 3.0 – The Human Metabolome Database in 2013. Nucleic Acids Res. 2013. Jan 1;41(D1):D801-7. 23161693.

6. Optimization of crystallization conditions for biological macromolecules. Alexander McPherson and Bob Cudney. Acta Crystallographica Section F Volume 70, Issue 11, pages 1445-1467, November 2014.

7. Screening and optimization strategies for macromolecular crystal growth. Cudney R, Patel S, Weisgraber K, Newhouse Y, McPherson A. Acta Crystallogr D Biol Crystallogr. 1994 Jul 1;50(Pt 4):414-23.

GRAS Additive GRAS Additive

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Heavy Atom Screens蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Heavy Atom Screens
Heavy Atom Screens

Heavy Atom Screens

Heavy Atom Screens

Heavy Atom Screens

Heavy Atom Screens

Products > Optimization Screens > Heavy Atom Screens > Heavy Atom Screens

Heavy Atom Screens

Applications

  • Heavy atoms for multiple isomorphous replacement

Features

  • Convenient sets of popular heavy atoms
  • Each kit include a heavy atom tutorial and formulation guide

Description

A Heavy Atom Screen kit provides 50 mg of each heavy atom in an o-ring screw cap micro tube. This convenient format provides sufficient material for the preparation of numerous (15 x 100 mM or 1,500 x 1 mM) small volume (0.1 ml) fresh stock solutions of heavy atoms. The quantity is sufficient for screening and derivatization but avoids the problem of storing large quantities of of heavy atoms.



Heavy Atom Screens

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Heavy Atom Screens

Heavy Atom Screens

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Heavy Atom Screens

CAT NO

HR2-442

NAME

Heavy Atom Screen Pt

DESCRIPTION

50 mg, tube format

PRICE

$587.00

cart quote

CAT NO

HR2-444

NAME

Heavy Atom Screen Au

DESCRIPTION

50 mg, tube format

PRICE

$403.00

cart quote

CAT NO

HR2-446

NAME

Heavy Atom Screen Hg

DESCRIPTION

50 mg, tube format

PRICE

$447.00

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CAT NO

HR2-448

NAME

Heavy Atom Screen M1

DESCRIPTION

50 mg, tube format

PRICE

$490.00

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CAT NO

HR2-450

NAME

Heavy Atom Screen M2

DESCRIPTION

50 mg, tube format

PRICE

$462.00

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Support Material(s)

Heavy Atom Screens HR2-442 Heavy Atom Screen Pt SDSHeavy Atom Screens HR2-444 Heavy Atom Screen Au SDSHeavy Atom Screens HR2-442 HR2-444 HR2-446 HR2-448 HR2-450 Heavy Atom Screens User GuideHeavy Atom Screens HR2-446 Heavy Atom Screen Hg SDSHeavy Atom Screens HR2-448 Heavy Atom Screen M1 SDSHeavy Atom Screens HR2-450 Heavy Atom Screen M2 SDS

Related Item(S)

  • Individual Heavy Atom Pt Reagents
  • Individual Heavy Atom Screen Hg Reagents
  • Individual Heavy Atom Screen M1 Reagents
  • Individual Heavy Atom M2 Reagents

References

1. Towards a rational approach for heavy-atom derivative screening in protein crystallography. Johnson Agniswamy, M. Gordon Joyce, Carl H. Hammer, and Peter D. Sun. Acta Cryst. (2008) D64, 354-367.

2. Heavy-atom derivatization. Elspeth Garman and James W. Murray, Acta Cryst. (2003). D59, 1903-1913.

3. Screening for phasing atoms in protein crystallography. Titus J Boggon and Lawrence Shapiro. Structure 2000, Vol 8 No 7, 143-149.

4. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part II: phasing of new structures. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1099-103. Epub 2002 Jun 20. Sun PD, Radaev S.

5. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part I: test cases. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1092-8. Epub 2002 Jun 20. Sun PD, Radaev S, Kattah M.

6. Preparation of isomorphous heavy-atom derivatives. Methods Enzymol. 1985;114:147-56. Petsko GA. PMID: 4079763

7. An overview of heavy-atom derivatization of protein crystals. A. C. W. Pike, E. F. Garman, T. Krojer, F. von Delft & E. P. Carpenter (2016). Acta Cryst. D72, 303-318.

8. Fluorescence Detection of Heavy Atom Labeling (FD-HAL): a rapid method for identifying covalently modified cysteine residues by phasing atoms.Chaptal V, Ujwal R, Nie Y, Watanabe A, Kwon S, Abramson J. J Struct Biol. 2010 Jul;171(1):82-7.

9. A rapid and rational approach to generating isomorphous heavy-atom phasing derivatives. Lu J, Sun PD. FEBS J. 2014 Sep;281(18):4021-8.

10. A rational approach to heavy-atom derivative screening. Joyce MG, Radaev S, Sun PD. Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):358-65.

Heavy Atom Screens Heavy Atom Screens

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Index • Index HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Index • Index HT
Index • Index HT

Index • Index HT

Index • Index HT

Index • Index HT

Index • Index HT

Products > Crystallization Screens > Index > Index • Index HT

Index • Index HT

Applications

  • Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules

Features

  • Developed at Hampton Research
  • A data-driven biased sparse matrix and grid screen
  • Screens classic, contemporary, & modern crystallization reagents
  • Samples pH 3 to 9
  • Compatible with microbatch, vapor diffusion, & liquid diffusion methods
  • Specially formulated reagent zones:
    • Traditional salts versus pH
    • Neutralized organic acids
    • High [salt] with low [polymer]
    • High [polymer] with low [salt]
    • Low ionic strength versus pH
    • PEG & Salt versus pH
    • PEG & Salt
  • Tube or Deep Well block format

Description

Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format. 

Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in producing crystals or limiting sample solubility. Results from Index can be used to design optimization experiments and to identify follow on screens by reagent class. For example, positive results with salt based reagents in Index may be followed up with further screening using SaltRx or Grid Screen Salt HT. Success with polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.

Index utilizes a broad, yet refined portfolio of crystallization reagent systems. These include the following: (1) traditional salts such as Ammonium sulfate and Sodium chloride versus pH; (2) neutralized organic acids such as Sodium malonate and Tacsimate; (3) High salt concentration mixed with low polymer concentration as well as high polymer concentration mixed with low salt concentration and; (4) Low ionic strength using polymers such as PEG, MPD, Pentaerythritols versus pH. These reagent systems are formulated across a sparse matrix and incomplete factorial of concentration ranges, sampling a pH range of 3 to 9.

Index contains 96 unique reagents, 10 ml each.

Index HT contains 96 unique reagents in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Measured pH range of kit is 3 to 9 at 25°C
Average measured pH of kit is 6.8 at 25°C
Median measured pH of kit is 6.9 at 25°C

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Index • Index HT

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Index • Index HT
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Index • Index HT
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Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT

CAT NO

HR2-144

NAME

Index

DESCRIPTION

10 ml, tube format

PRICE

$550.00

cart quote

CAT NO

HR2-134

NAME

Index HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

Index • Index HT HR2-144 Index DocumentsIndex • Index HT HR2-144 Index SDSIndex • Index HT HR2-134 Index HT DocumentsIndex • Index HT HR2-134 Index HT SDSIndex • Index HT Index & Index HT Formulations & Scoring

Related Item(S)

  • Individual Index • Index HT Reagents

References

1. The advantages of using a modified microbatch method for rapid screening of protein crystallization conditions. A. D′Arcy, A. Mac Sweeney, M. Stihle and A. Haber. Acta Cryst. (2003). D59, 396-399.

2. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of propionate kinase (TdcD) from Salmonella typhimurium. D. K. Simanshu and M. R. N. Murthy. Acta Cryst. (2005). F61, 52-55.

3. Preparation, crystallization and preliminary X-ray analysis of the methionine synthase (MetE) from Streptococcus mutans. T.-M. Fu, X.-Y. Zhang, L.-F. Li, Y.-H. Liang and X.-D. Su. Acta Cryst. (2006). F62, 984-985.

4. Crystallization and preliminary X-ray analysis of glutathione transferases from cyanobacteria. S. C. Feil, J. Tang, G. Hansen, M. A. Gorman, E. Wiktelius, G. Stenberg and M. W. Parker. Acta Cryst. (2009). F65, 475-477.

Index • Index HT Index • Index HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Ionic Liquid Screen蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Ionic Liquid Screen
Ionic Liquid Screen

Ionic Liquid Screen

Ionic Liquid Screen

Ionic Liquid Screen

Ionic Liquid Screen

Products > Optimization Screens > Ionic Liquid Screen > Ionic Liquid Screen

Ionic Liquid Screen

Applications

  • Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility
  • Additive screen for protein crystallization

Features

  • 24 unique ionic liquids
  • 0.5 ml tube format

Description

Ionic Liquid Screen is a kit designed to allow the rapid and convenient evaluation of 24 unique ionic liquids and their ability to influence the crystallization of the sample. The screen is designed to be compatible with most crystallization reagents including all reagents utilized in all of the Hampton Research screens.

Ionic liquids have been found effective as additives in protein crystallization, with different ionic liquids used to increase crystallization rates and crystal size.1-4 The inclusion of ionic liquids in crystallization experiments has been reported to lead to less crystal polymorphism as well as less precipitation at higher precipitant concentrations.25 Ionic liquids have been used as additives to produce crystals in reagents that had previously not resulted in crystallization and results suggest ionic liquids may be applicable for the solubilization and crystallization of membrane proteins.2

Ionic liquids are organic salts with melting points below 100°Celsius. They are thermally stable, nonflammable and demonstrate very low vapor pressure. Ionic liquids are soluble in a variety of organic and inorganic reagents and can be highly water soluble. Ionic liquids can demonstrate a degree of localized structuring about each ion compared to materials composed of disassociated ions, setting them apart from salt solutions.5-6 Ionic liquids can participate in ionic, hydrophobic and hydrogen bond interactions. Ionic liquids are often chaotropic, composed of low symmetry ions with charge delocalization and weak intermolecular interactions.1 These organic salts generally consist of combinations of organic cations and either an organic or inorganic anion. Ionic liquids have been demonstrated to suppress protein aggregation and significantly increase protein folding yields.7-8 Ionic liquids have been reported to stabilize protein activity and structure.9-11 The inclusion of the ionic liquid 1-n-Butyl-3-methylimidazolium tetrafluoroborate (included in this kit) improved the thermal stability and solubility of integral membrane proteins for membrane proteomics study.12

Some ionic liquids, such as ethylammonium nitrate have water-like characteristics, including the capacity for hydrogen bonding and the promotion of micelle formation by some surfactants.13 Many ionic liquids are also organics acids and have ionic character in addition to the hydrophobic behavior, which makes them unique and useful solvents in protein chemistry.

Variation of the anion and the cation as well as the utilization of both soft (formate and acetate) and hard anions (nitrate) in the Ionic Liquid Screen reagents provides an additional dimension for evaluating the effects on ionic liquids on the solubility and crystallization of proteins. The Ionic Liquid Screen contains a set of 24 water soluble ionic liquids that comprise different cation (imidazolium, phosphonium, ammonium) and anion (borate, halides, sulfates, acetates, sulfonates, nitrates) structures for a diverse ionic liquid additive screen for use in improving the crystallization behavior and X-ray diffraction resolution of proteins.

Each of the 24 ionic liquids are preformulated in deionized water and sterile filtered using a 0.2 micron filter. Ionic liquid Screen contains 24 unique reagents, 0.5 milliliter each.

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Ionic Liquid Screen

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Ionic Liquid Screen
Ionic Liquid Screen
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Ionic Liquid Screen

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Ionic Liquid Screen
Ionic Liquid Screen
Ionic Liquid Screen

CAT NO

HR2-214

NAME

Ionic Liquid Screen

DESCRIPTION

0.5 ml, tube format

PRICE

$310.00

cart quote

Support Material(s)

Ionic Liquid Screen HR2-214 Ionic Liquid Screen DocumentsIonic Liquid Screen HR2-214 Ionic Liquid SDSIonic Liquid Screen Ionic Liquid Screen Formulation and Scoring Data

Related Item(S)

  • Individual Ionic Liquid Reagents

References

1. Pusey, M.L., Paley, M.S., Turner, M.B., and Rogers, R.D. 2007. Protein crystallization using room temperature ionic liquids. Crystal Growth & Design. 74:787-793.

2. Hekmat, D., Hebel, D., Sebastian, J. Schmidt, M., and Weuster-Botz, D. 2007. Advanced protein crystallization using water-soluble ionic liquids as crystallization additives. Biotech. Lett., 29:703-1711.

3. Garlitz, J.A., Summers, C.A., Flowers, R.A. and Borgstahl, G.E.O. 1999. Ethylammonium nitrate: A protein crystallization reagent. Acta Cryst. D53:2037-2038.

4. Judge, R.A., Takahashi, S., Longnecker, K.L. Fry, E.H., Abad-Zapatero, C., and Chi, M.L. 2009. The effects of ionic liquids on protein crystallization and X-ray diffraction resolution. Crystal Growth & Design 9:3463-3469.

5. Bowran, D.T., Hardacre, C. Holbrey, J.D., McMath, J.E., and Soper, A.K. 2003. J. Chem. Phys. 118:173-178.

6. Cadena, C., Zhao, Q., Snurr, and R.Q., Maginn, E.J. 2006. J. Chem. Phys. B 110:2821-2832.

7. Summers, C.A. and Flowers, R.A. 2000. Protein renaturation by the liquid organic salt ethylammonium nitrate. Protein Science 9:2001-2008.

8. Lange, C., Patil, G., and Rudolph, R. 2005. Ionic liquids as refolding additives: N′-alkyl and N′-(w-hydroxyalkyl) N-methylimidazolium chloride. Protein Science 14:2693-2701.

9. Lozano, P. , de Diego, T. Guegan, J.P., Vaultier, M., and Ibora, J.L. 2001. Stabilization of a-chymotrypsin by ionic liquids in transesterification reactions. Biotehnol. Bioeng. 75:363-369.

10. Baker, S.N., McCleskey, T.M., Pandy, S., and Baker, G.A. 2004. Fluorescence study of protein thermostability in ionic liquids. Chem. Commun. 2004:940-941.

11. De Diego, T., Lozano, P., Gmouth, S. Vaultier, M., and Iborra, J.L. 2004. Fluorescence and CD spectroscopic analysis of the a-chymotrypsin stabilization by the ionic liquid 1-ethyl-3-methyimidazolium bis[(trifluormethyl)sulfonyl]amide. Biotechnol. Bioeng. 88:916-924.

12. Sun, L., Tao, D., Han, B., Ma, J., Zhu, G., Liang, Z., Shan, Y., Zhang, L., and Zhang, Y. 2010. Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics. Anal. Bioanal. Chem. DOI 10.1007/s0216-010-4381-5.

13. Evans, D.F., Yamauchi, A., Roman, R., and Casassa, E.Z. 1982. J. Colloids Interface Sci. 88:89-96.

14. Proteins in Ionic Liquids: Current Status of Experiments and Simulations. Christian Schrode, Top Curr Chem (J). 2017; 375(2): 25.

15. Ionic Liquids as Stabilization and Refolding Additives and Solvents for Proteins. Fujita K. Adv Biochem Eng Biotechnol. 2018 Jul 13. doi: 10.1007/10_2018_65.

16. Fujita K. (2018) Ionic Liquids as Stabilization and Refolding Additives and Solvents for Proteins. In: . Advances in Biochemical Engineering/Biotechnology. Springer, Berlin, Heidelberg.

Ionic Liquid Screen Ionic Liquid Screen

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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MembFac • Crystal Screen Lite • MembFac HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

Products > Crystallization Screens > MembFac • Crystal Screen Lite > MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

Applications

  • Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility

Features

  • Membrane protein sparse matrix screens
  • Sparse matrix formula efficiently samples salts, polymers, organics and pH
  • pH range 4.6 – 8.5
  • Formulated for use with detergents
  • Tube or Deep Well block format
  • Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

Description

MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins.

MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.

MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.

The Rationale The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.

The Method The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.

The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.

Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2 Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.

MembFac contains 48 unique reagents, 10 ml each.

MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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MembFac • Crystal Screen Lite • MembFac HT
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MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

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MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
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MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT

CAT NO

HR2-114

NAME

MembFac

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-128

NAME

Crystal Screen Lite

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-137

NAME

MembFac HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

MembFac • Crystal Screen Lite • MembFac HT HR2-114 MembFac DocumentsMembFac • Crystal Screen Lite • MembFac HT HR2-114 MembFac SDSMembFac • Crystal Screen Lite • MembFac HT HR2-128 Crystal Screen Lite DocumentsMembFac • Crystal Screen Lite • MembFac HT HR2-128 Crystal Screen Lite SDSMembFac • Crystal Screen Lite • MembFac HT HR2-137 MembFac HT DocumentsMembFac • Crystal Screen Lite • MembFac HT HR2-137 MembFac HT SDSMembFac • Crystal Screen Lite • MembFac HT MembFac & Crystal Screen Lite Formulation & Scoring Data

Related Item(S)

  • Individual MembFac • Crystal Screen Lite • MembFac HT Reagents

References

1. UCLA Crystallization Workshop, June 21, 1993.

2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.

3. McPherson, A., Current approaches to macromolecular crystallization., Eur. J. Biochem. (1990) 189, 1-23.

4. Jancarik, J. and Kim, S.H., Sparse Matrix Sampling: a screening method for crystallization of proteins. (1991) J. Appl. Cryst., 24,409-411.

5. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.

6. Structural and functional characterization of the N-terminal domain of the yeast Mg2+ channel Mrs2. M. B. Khan, G. Sponder, B. Sjoblom, S. Svidova, R. J. Schweyen, O. Carugo and K. Djinovic-Carugo. Acta Cryst. (2013). D69, 1653-1664.

MembFac • Crystal Screen Lite • MembFac HT MembFac • Crystal Screen Lite • MembFac HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Natrix • Natrix 2 • Natrix HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Products > Crystallization Screens > Natrix • Natrix 2 > Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Applications

  • Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes

Features

  • Nucleic acid sparse matrix screen
  • Sparse matrix formulation efficiently samples salts, polyols, polymers, organics, additives & pH
  • pH range 5.6 – 8.5
  • Tube or Deep Well block format

Description

Natrix, Natrix 2 and Natrix HT are based upon published reagent formulations for the crystallization of nucleic acids and protein-nucleic acid complexes. A variety of hammerhead ribozymes and other ribozymes, RNAs, DNAs, RNA-drug complexes, and RNA-protein complexes have been crystallized using the Natrix protocols.

By using sparse matrix sampling technology, The Natrix kits allow one to quickly test wide ranges of pH, salts, and precipitants using a very small sample of nucleic acid.

Natrix screens are unique in that rather than relying solely on the traditional nucleic acid precipitant MPD, Natrix screens also utilize Polyethylene glycols (PEGs) in a variety of molecular weights (200, 400, 4,000, 8,000) as well as 2-Propanol, Polyethylene glycol monomethyl ether (PEG MME), and 1,6-Hexanediol. Many of the polymeric and low molecular weight organic precipitants are combined with various monovalent salts as precipitating agents. This combination of salts and low molecular weight organics and polyalcohols, as well as the utilization of varying chain length PEGs, has proven to be a successful combination for producing nucleic acid and protein-nucleic acid complex crystals.

Natrix contains 48 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by William Scott in 1995.

Natrix 2, an extension of Natrix, contains 48 unique reagents, 10 ml each. Natrix 2 is a biased sparse matrix screen based on extracting patterns from crystallization data as well as reagent formulations first described by Berger et al in 1996.

Natrix HT contains 1 ml of each reagent from Natrix and Natrix 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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Natrix • Natrix 2 • Natrix HT
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Natrix • Natrix 2 • Natrix HT

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Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT

CAT NO

HR2-116

NAME

Natrix

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-117

NAME

Natrix 2

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-131

NAME

Natrix HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

Natrix • Natrix 2 • Natrix HT HR2-116 Natrix DocumentsNatrix • Natrix 2 • Natrix HT HR2-116 Natrix SDSNatrix • Natrix 2 • Natrix HT HR2-117 Natrix 2 DocumentsNatrix • Natrix 2 • Natrix HT HR2-117 Natrix 2 SDSNatrix • Natrix 2 • Natrix HT HR2-131 Natrix HT DocumentsNatrix • Natrix 2 • Natrix HT HR2-131 Natrix HT SDSNatrix • Natrix 2 • Natrix HT Changes in Formulation Natrix Natrix 2 Natrix HTNatrix • Natrix 2 • Natrix HT Natrix Formulation & Scoring Data

Related Item(S)

  • Individual Natrix • Natrix 2 • Natrix HT Reagents

References

1. Scott, W. G., Finch, J.T., Grenfell, R., Fogg, J., Smith, T., Gait, M.J., Klug, A., Journal of Molecular Biology (1995) 250: 327-332.

2. Purification, crystallization and preliminary X-ray analysis of the BseCI DNA methyltransferase from Bacillus stearothermophilus in complex with its cognate DNA. E. G. Kapetaniou, D. Kotsifaki, M. Providaki, M. Rina, V. Bouriotis and M. Kokkinidis. Acta Cryst. (2007). F63, 12-14.

3. Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Forster, M. Perbandt, A. B. E. Brauer, S. Brode, J. P. Furste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.

4. Combinatorial crystallization of an RNA-protein complex. Danielle Bodrero Hoggan, Jeffrey A. Chao, G. S. Prasad, C. David Stouta and James R. Williamson. Acta Cryst (2003). D59, 466-473.

5. Cocrystallizing natural RNA with its unnatural mirror image: biochemical and preliminary X-ray diffraction analysis of a 5S rRNA A-helix racemate. Volker A. Erdmanna et al. Acta Cryst. (2007). F63, 839-843.

6. Berger, et al., A Highly Effective 24 Condition Matrix for the Crystallization of Nucleic Acid Fragments. Acta Cryst. Section D. (1996) Vol. D52 Part 3, 465-468.

7. Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Förster, M. Perbandt, A. B. E. Brauer, S. Brode, J. P. Fürste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.

8. Adams, A., Nucleic Acids Research (2002) Vol. 30, 719-725.

9. Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-b receptor promoter DNA. Agarkar et al. Acta Cryst. (2009). F65, 1261-1263.

10. Crystallization and preliminary X-ray crystallographic studies of transglutaminase 2 in complex with Ca2+. T.-H. Jang and H. H. Park. Acta Cryst. (2014). F70, 513-516 [ doi:10.1107/S2053230X1400510X ]

Natrix • Natrix 2 • Natrix HT Natrix • Natrix 2 • Natrix HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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PCT™ Pre-Crystallization Test蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

Products > Crystallization Screens > PCT Pre-Crystallization Test > PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

Applications

  • Determine the appropriate sample concentration for crystallization screening

Features

  • Conserve sample
  • Optimize sample concentration prior to initial screens
  • Provide insight to sample homogeneity

Description

The PCT Pre-Crystallization Test is used to determine the appropriate protein concentration for crystallization screening. Sample concentration is a significant crystallization variable. Samples too concentrated can result in amorphous precipitate, while samples too dilute can result in clear drops. Precipitate and clear drops are typical crystallization screen results for reagent conditions which do not promote crystallization and are part of every crystallization screen. However, by optimizing protein concentration for the screen, the number of clear and precipitate results can often be reduced, which in turn results in more efficient sample utilization while at the same time enhancing the chances for crystallization. PCT can minimize or prevent situations where a screen results in an over abundance of precipitate or clear drops.

The PCT kit contains 4 unique, preformulated, sterile filtered reagents used to evaluate protein concentration for crystallization screening. Initially, the sample protein is mixed with two of the reagents to determine if the protein concentration is appropriate for crystallization screening. If the protein is very sensitive to salt and polymer concentration, based on initial PCT results, the protein may be evaluated using a second set of PCT reagents. PCT results will then provide insight to either the appropriate sample concentration or indicate that other diagnostic testing such as native gel electrophoresis or dynamic light scattering should be performed to demonstrate sample homogeneity appropriate for crystallization.

PCT Kit comparison table.

Catalog
Number
4 Unique
Reagents
Glass Siliconized
Cover Slides
24 Well Plate
with sealant
HR2-140          (50 mL each)          (22 mm Square)  
HR2-141          (10 mL each)    
HR2-142          (30 mL each)          (22 mm Square)          (5x VDX)
HR2-143          (10 mL each)          (18 mm Circle)          (4x VDXm)

 

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PCT™ Pre-Crystallization Test

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PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

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PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test

CAT NO

HR2-140

NAME

PCT (with slides)

DESCRIPTION

50 ml bottles (4 ea), cover slides (1 pk)

PRICE

$109.00

cart quote

CAT NO

HR2-141

NAME

PCT (reagents only)

DESCRIPTION

10 ml tubes (4 ea)

PRICE

$77.00

cart quote

CAT NO

HR2-142

NAME

PCT (with plates and slides)

DESCRIPTION

30 ml bottles (4 ea), cover slides (1 pk), VDX Plates with sealant (5 ea)

PRICE

$132.00

Temporarily Unavailable
More Available Soon!

CAT NO

HR2-143

NAME

PCT (with plates and slides)

DESCRIPTION

10 ml tubes (4 ea), cover slides (1 pk), VDXm Plates with sealant (4 ea)

PRICE

$99.00

cart quote

Support Material(s)

PCT™ Pre-Crystallization Test HR2-140 & HR2-141 & HR2-142 & HR2-143 PCT Kit DocumentsPCT™ Pre-Crystallization Test HR2-140 PCT Kit (with slides) SDSPCT™ Pre-Crystallization Test HR2-141 PCT Kit (reagents only) SDSPCT™ Pre-Crystallization Test HR2-142 PCT Kit (with plates and slides) SDSPCT™ Pre-Crystallization Test HR2-143 PCT Kit (with plates and slides) SDS

Related Item(S)

  • Individual PCT Reagents

References

1. Crystallization and x-ray diffraction analysis of human CLEC-2. Aleksandra A. Watson and Christopher A. O’Callaghan. Acta Cryst. (2005). F61, 1094-1096.

PCT™ Pre-Crystallization Test PCT™ Pre-Crystallization Test

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Products > Crystallization Screens > PEG/Ion • PEG/Ion 2 > PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Applications

  • Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules

Features

  • Developed at Hampton Research
  • PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 – 9.2
  • PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 – 8.8) in the presence of monodisperse PEG 3,350
  • PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block

Description

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules.

The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.

PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.

PEG/Ion contains 48 unique reagents, 10 ml each.

PEG/Ion 2 contains 48 unique reagents, 10 ml each.

PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
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PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

CAT NO

HR2-126

NAME

PEG/Ion Screen

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-098

NAME

PEG/Ion 2 Screen

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-139

NAME

PEG/Ion HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

PEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-126 PEG/Ion DocumentsPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-126 PEG/Ion SDSPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-098 PEG/Ion 2 DocumentsPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-098 PEG/Ion 2 SDSPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-139 PEG/Ion HT DocumentsPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-139 PEG/Ion HT SDSPEG/Ion • PEG/Ion 2 • PEG/Ion HT PEG/Ion Formulation & Scoring Data

Related Item(S)

  • Individual PEG/Ion • PEG/Ion 2 • PEG/Ion HT Reagents

References

1. Expression, purification, crystallization and preliminary X-ray diffraction analysis of Bifidobacterium adolescentis xylose isomerase. C. V. dos Reis, A. Bernardes and I. Polikarpov. Acta Cryst. (2013). F69, 588-591.

2. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

3. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology Volume 156, Issue 3 , December 2006, Pages 387-406.

4. Mechanisms for Glycolipid Antigen-Driven Cytokine Polarization by Valpha14i NKT Cells. Sulliivan et al, The Journal of Immunology, 2010, 184, 141 -153. (PEG/Ion 2)

5. Optimization of salt concentration in PEG-based crystallization solutions. Yamanaka M, Inaka K, Furubayashi N, Matsushima M, Takahashi S, Tanaka H, Sano S, Sato M, Kobayashi T, Tanaka T. J Synchrotron Radiat. 2011 Jan;18(1):84-7. doi: 10.1107/S0909049510035995. Epub 2010 Nov 5.

6. Crystallization and preliminary X-ray crystallographic analysis of importin-[alpha] from Neurospora crassa. N. E. Bernardes, A. A. S. Takeda, F. Z. Freitas, M. C. Bertolini and M. R. M. Fontes. Acta Cryst. (2014). F70, 501-504 [ doi:10.1107/S2053230X14005068 ]

PEG/Ion • PEG/Ion 2 • PEG/Ion HT PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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PEG/Ion 400蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

PEG/Ion 400
PEG/Ion 400

PEG/Ion 400

PEG/Ion 400

PEG/Ion 400

PEG/Ion 400

Products > Crystallization Screens > PEG/Ion 400 > PEG/Ion 400

PEG/Ion 400

Applications

  • Crystallization screen for membrane and soluble biological macromolecules.

Features

  • Reagent formulation developed at Hampton Research
  • Screens a profile of anions, cations, multivalent ions, and neutralized organic acids at varying pH levels in the presence of Polyethylene glycol 400
  • pH range 5 – 9
  • Buffer free formulation
  • Compatible with lipidic cubic phase (LCP), vapor diffusion, microbatch, and free interface diffusion

Description

PEG/Ion 400 is a crystallization reagent kit designed to provide a rapid screening method for the crystallization of membrane and soluble biological macromolecules.

Most G protein-coupled receptors (GPCRs) have been crystallized in Polyethylene glycol (PEG) 400 based reagents1. PEG/Ion 400 is designed as a 96 reagent crystallization screen that is compatible with the Lipidic Cubic Phase (LCP).

PEG/Ion 400 is supplied in a sterile, polypropylene 96 Deep Well block, each reservoir containing 1 ml of sterile filtered reagent. The block is compatible with robotic and multi-channel pipet liquid handling systems and is heat sealed using a special polypropylene backed film. Each PEG/Ion 400 is supplied with an adhesive sealing film which can be used to seal the block after removing the heat seal.

The screen combines a single concentration (30% v/v) of high purity Polyethylene glycol 400 and 48 different high purity salts, comprising both anions (malate, malonate, nitrate, phosphate, succinate, sulfate, tartrate, and thiocyanate) and cations (ammonium, cadmium, calcium, ethylammonium, lithium, magnesium, nickel, potassium, sodium, tetraethylammonium, and zinc) in two concentrations (0.1 and 0.4 M) which due to their unique pH characteristics also affords a reasonable pH screen (approximate pH range of 4 to 9.5). The primary screen variables are PEG, ion type, ionic strength, salt concentration, and pH.

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PEG/Ion 400

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PEG/Ion 400
PEG/Ion 400

PEG/Ion 400

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PEG/Ion 400
PEG/Ion 400
PEG/Ion 400

CAT NO

HR2-460

NAME

PEG/Ion 400

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

PEG/Ion 400 HR2-460 PEG/Ion 400 DocumentsPEG/Ion 400 HR2-460 PEG/Ion 400 SDSPEG/Ion 400 PEG/Ion 400 Formulation & Scoring Data

Related Item(S)

  • Individual PEG/Ion 400 Reagents

References

1. Successful Strategies to Determine High-Resolution Structures of GPCRs. Xiang J, Chun E, Liu C, Jing L, Al-Sahouri Z, Zhu L, Liu W. Trends Pharmacol Sci. 2016 Dec;37(12):1055-1069.

2. Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. Kang Y, Zhou XE, Gao X, et al. Nature. 2015;523(7562):561-567. doi:10.1038/nature14656.

3. Development of an Automated High Throughput LCP-FRAP Assay to Guide Membrane Protein Crystallization in Lipid Mesophases. Fei Xu, Wei Liu, Michael A. Hanson, Raymond C. Stevens, and Vadim Cherezov. Cryst. Growth Des., 2011, 11 (4), pp 1193-1201.

4. A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes. Martin Caffrey. Acta Crystallogr F Struct Biol Commun. 2015 Jan 1; 71(Pt 1): 3-18.

PEG/Ion 400 PEG/Ion 400

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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PEGRx 1 • PEGRx 2 • PEGRx HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

Products > Crystallization Screens > PEGRx 1 • PEGRx 2 > PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

Applications

  • Primary and secondary, polymer and pH based crystallization screen for biological macromolecules

Features

  • Developed at Hampton Research
  • PEGRx 1 primary screen variables are polymer type (16 different polymers), polymer molecular weight, pH and low ionic strength.
  • PEGRx 2 primary screen variables are polymer type (13 different polymers), polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.
  • PEGRx HT combines PEGRx 1 and PEGRx 2 into a single 96 Deep Well block
  • pH range 3.5 – 9, using 10 different buffer systems
  • Polymer molecular weight range 200 to 20,000
  • Ligand friendly, complex friendly

Description

PEGRx is a primary and secondary, polymer and pH based crystallization screen developed at Hampton Research.

It is designed to evaluate polymer based crystallization reagents and pH in low (PEGRx 1) to medium ionic strength (PEGRx 2). It is also designed for use as a secondary or optimization screen to follow the Hampton Research Index screen when polymer based reagents produce hits and interesting solubility leads.

PEGRx 1 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a low ionic strength environment versus a wide range of pH. Polymer reagents include Polyethylene glycols, Polyethylene glycol monomethyl ethers, and Jeffamines®. The molecular weight range between 200 and 20,000 is evaluated in a low ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and low ionic strength.

PEGRx 2 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a medium ionic strength environment in the presence of additives, salts, volatile organics and polyols versus a wide range of pH. Polymer reagents include Polyethylene glycols and Polyethylene glycol monomethyl ethers. The polymer molecular weight range between 200 and 20,000 is evaluated in a medium ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.

PEGRx 1 contains 48 unique reagents, 10 ml each.

PEGRx 2 contains 48 unique reagents, 10 ml each.

PEGRx HT contains the 96 PEGRx 1 and PEGRx 2 reagent in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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PEGRx 1 • PEGRx 2 • PEGRx HT

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PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

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PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT

CAT NO

HR2-082

NAME

PEGRx 1

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-084

NAME

PEGRx 2

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-086

NAME

PEGRx HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

PEGRx 1 • PEGRx 2 • PEGRx HT HR2-082 PEGRx 1 DocumentsPEGRx 1 • PEGRx 2 • PEGRx HT HR2-082 PEGRx 1 SDSPEGRx 1 • PEGRx 2 • PEGRx HT HR2-084 PEGRx 2 DocumentsPEGRx 1 • PEGRx 2 • PEGRx HT HR2-084 PEGRx 2 SDSPEGRx 1 • PEGRx 2 • PEGRx HT HR2-086 PEGRx HT DocumentsPEGRx 1 • PEGRx 2 • PEGRx HT HR2-086 PEGRx HT SDSPEGRx 1 • PEGRx 2 • PEGRx HT Change in Formulation PEGRx ScreensPEGRx 1 • PEGRx 2 • PEGRx HT PEGRx Formulation & Scoring Data

Related Item(S)

  • Individual PEGRx 1 • PEGRx 2 • PEGRx HT Reagents

References

1. Crystallization and preliminary X-ray crystallographic studies of DesR, a thermosensing response regulator in a two-component signalling system from Streptococcus pneumoniae. Ae Kyung Park, Seung Min Bong, Jin Ho Moon and Young Min Chi. Acta Cryst. (2009). F65.

2. Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors.  Zhang L, Lin D, Sun X, Curth U, Drosten C, Sauerhering L, Becker S, Rox K, Hilgenfeld R. Science. 2020 Apr 24;368(6489):409-412. doi: 10.1126/science.abb3405. Epub 2020 Mar 20.

3. Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase.  Zhang, L., Xie, Z., Liu, Z. et al.  Nat Commun 11, 2676 (2020). https://doi.org/10.1038/s41467-020-16500-5.

PEGRx 1 • PEGRx 2 • PEGRx HT PEGRx 1 • PEGRx 2 • PEGRx HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Proti-Ace • Proti-Ace 2蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Proti-Ace • Proti-Ace 2
Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Products > Optimization Screens > Proti-Ace > Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Applications

  • Limited proteolysis, in situ proteolysis, and proteolytic screening of protein samples for crystallization and structure determination

Features

  • Proti-Ace proteases
    • alpha-Chymotrypsin
    • Trypsin
    • Elastase
    • Papain
    • Subtilisin
    • Endoproteinase Glu-C
  • Proti-Ace 2 proteases
    • Proteinase K
    • Clostripain (Endoproteinase-Arg-C)
    • Pepsin
    • Thermolysin
    • Bromelain
    • Actinase E
  • Optimized, stable, freeze dried protease formulation

Description

A proteolytic fragment or domain of a protein may crystallize more readily or form better diffracting crystals than the intact protein.1-8

Proteases can be used to generate small, active fragments or domains of the target protein for crystallization.9 The fragment or domain can be used directly for crystallization experiments. Or the proteolytic sample analyzed by gel electrophoresis and/or mass spectrometry for mass and sequence for subsequent cloning, expression, purification and crystallization. Using proteolysis to enhance sample crystallization, the current overall success rate for yielding a deposited crystal structure is currently better than 12%.3

Proti-Ace (HR2-429) contains three aliquots of 6 unique proteases (a-Chymotrypsin, Trypsin, Elastase, Papain, Subtilisin and Endoproteinase Glu-C) and six aliquots of Proti-Ace Dilution Buffer. Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.

Proti-Ace 2 (HR2-432) contains three aliquots of 6 unique proteases (Proteinase K, Clostripain (Endoproteinase-Arg-C), Pepsin, Thermolysin, Bromelain and Actinase E) and six aliquots of Proti-Ace Dilution Buffer. Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.

The unique freeze dried formulation of the Proti-Ace kits offers a much improved protease stability compared to liquid protease formulations.

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Proti-Ace • Proti-Ace 2
Proti-Ace • Proti-Ace 2
Proti-Ace • Proti-Ace 2
Proti-Ace • Proti-Ace 2

CAT NO

HR2-429

NAME

Proti-Ace™

DESCRIPTION

tube format

PRICE

$274.00

cart quote

CAT NO

HR2-432

NAME

Proti-Ace™ 2

DESCRIPTION

tube format

PRICE

$274.00

cart quote

Support Material(s)

Proti-Ace • Proti-Ace 2 HR2-429 Proti-Ace User GuideProti-Ace • Proti-Ace 2 HR2-429 Proti-Ace SDSProti-Ace • Proti-Ace 2 HR2-432 Proti-Ace 2 User GuideProti-Ace • Proti-Ace 2 HR2-432 Proti-Ace 2 SDSProti-Ace • Proti-Ace 2 Proti-Ace Enzyme Mr pI Specificty Source

Related Item(S)

  • Individual Proti-Ace & Proti-Ace 2 Reagents

References

1. Allan D′Arcy, personal communication, 1989-2009.

2. In situ proteolysis for protein crystallization and structure determination. Dong, A et al. Nature Methods – 4, 1019 – 1021 (2007).

3. In Situ Proteolysis to Generate Crystals for Structure Determination: An Update. Amy Wernimont, Aled Edwards. PLoS ONE 4(4): e5094. doi:10.1371/ journal.pone.0005094.

4. The use of in situ proteolysis in the crystallization of murine CstF-77. Tong et al. Acta Cryst. (2007). F63, 135-138.

5. A brief history of protein crystal growth. McPherson, A. Journal of Crystal Growth, vol. 110, issue 1-2, pp. 1-10, 1991.

6. Preparation and analysis of protein crystals. McPherson, A. John Wiley & Sons, Inc. 1982. ISBN 089464355X.

7. A crystallizable form of the Streptococcus gordonii surface antigen SspB C-domain obtained by limited proteolysis. Forsgren et al. Acta Cryst. (2009). F65, 712-714.

8. Preliminary X-ray analysis of a human VH fragment at 1.8 angstrom resolution. Gaur, Kupper, Fischer & Hoffman. Acta Cryst. (2004). D60, 965-967.

9. Replication Protein A Characterization and Crystallization of the DNA Binding Domain. Pfuetzner et al. The Journal of Biological Chemistry, Vol. 272, No. 1, Issue of January 3, pp. 430-434, 1997.

10. Combining in situ proteolysis and mass spectrometry to crystallize Escherichia coli PgaB. Little et al. Acta Cryst. (2012) F68, 842-845.

11. Proteolysis of Native Proteins – Trapping of a Reaction Intermediate. Chenyi Wu, Duncan H. L. Robertson, Simon J. Hubbard, Simon J. Gaskell and Robert J. Beynon. doi: 10.1074/jbc.274.2.1108 January 8, 1999 The Journal of Biological Chemistry, 274, 1108-1115.

12. Crystallization of mouse RIG-I ATPase domain: in situ proteolysis. Civril F, Hopfner KP. Methods Mol Biol. 2014;1169:27-35. doi: 10.1007/978-1-4939-0882-0_3.

13. Crystallization and preliminary X-ray crystallographic analysis of YfcM: an important factor for EF-P hydroxylation. K. Kobayashi, T. Suzuki, N. Dohmae, R. Ishitani and O. Nureki. Acta Cryst. (2014). F70, 1236-1239 [ doi:10.1107/S2053230X14015726 ]. Synopsis: in situ proteolysis crystallization method produces crystals diffracting to 1.45 Å

Proti-Ace • Proti-Ace 2 Proti-Ace • Proti-Ace 2

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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SaltRx 1 • SaltRx 2 • SaltRx HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

SaltRx 1 • SaltRx 2 • SaltRx HT
SaltRx 1 • SaltRx 2 • SaltRx HT

SaltRx 1 • SaltRx 2 • SaltRx HT

SaltRx 1 • SaltRx 2 • SaltRx HT

SaltRx 1 • SaltRx 2 • SaltRx HT

SaltRx 1 • SaltRx 2 • SaltRx HT

Products > Crystallization Screens > SaltRx 1 • SaltRx 2 > SaltRx 1 • SaltRx 2 • SaltRx HT

SaltRx 1 • SaltRx 2 • SaltRx HT

Applications

  • Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules

Features

  • Salt versus pH matrix crystallization screen
  • Samples pH 4 to 9
  • 22 unique salts versus salt concentration and pH
  • Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods
  • Tube or Deep Well block format
  • Preformulated, ready to screen
  • Developed at Hampton Research
  • All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals
  • Individual reagents available through the Hampton Research Custom Shop

Description

SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules.

Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.

The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.

SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.

Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.

SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Measured pH range of kit is 4.1 to 9.0 at 25°C
Average measured pH of kit is 6.9 at 25°C
Median measured pH of kit is 7.3 at 25°C
Mode measured pH of kit is 7.4 at 25°C

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SaltRx 1 • SaltRx 2 • SaltRx HT
SaltRx 1 • SaltRx 2 • SaltRx HT
SaltRx 1 • SaltRx 2 • SaltRx HT

SaltRx 1 • SaltRx 2 • SaltRx HT

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SaltRx 1 • SaltRx 2 • SaltRx HT
SaltRx 1 • SaltRx 2 • SaltRx HT
SaltRx 1 • SaltRx 2 • SaltRx HT
SaltRx 1 • SaltRx 2 • SaltRx HT
SaltRx 1 • SaltRx 2 • SaltRx HT
SaltRx 1 • SaltRx 2 • SaltRx HT
SaltRx 1 • SaltRx 2 • SaltRx HT

CAT NO

HR2-107

NAME

SaltRx 1

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-109

NAME

SaltRx 2

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-136

NAME

SaltRx HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

SaltRx 1 • SaltRx 2 • SaltRx HT HR2-107 SaltRx 1 DocumentsSaltRx 1 • SaltRx 2 • SaltRx HT HR2-107 SaltRx 1 SDSSaltRx 1 • SaltRx 2 • SaltRx HT HR2-109 SaltRx 2 DocumentsSaltRx 1 • SaltRx 2 • SaltRx HT HR2-109 SaltRx 2 SDSSaltRx 1 • SaltRx 2 • SaltRx HT HR2-136 SaltRx HT DocumentsSaltRx 1 • SaltRx 2 • SaltRx HT HR2-136 SaltRx HT SDSSaltRx 1 • SaltRx 2 • SaltRx HT SaltRx Formulation & Scoring Data

Related Item(S)

  • Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

References

1. Cloning, expression, purification and preliminary X-ray diffraction studies of a mycobacterial protein implicated in bacterial survival in macrophages. A. E. Shahine, P. Y. Chan, D. Littler, J. Vivian, R. Brammananth, P. K. Crellin, R. L. Coppel, J. Rossjohn and T. Beddoe. Acta Cryst. (2013). F69, 566-569.

2. Gilliland, G.L., Tung, M., Blakeslee, D.M. and Ladner, J. 1994. The Biological Macromolecule Crystallization Database, Version 3.0: New Features, Data, and the NASA Archive for Protein Crystal Growth Data. Acta Crystallogr. D50 408-413.

3. Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus. S. P. Kanaujia, C. V. Ranjani, J. Jeyakanthan, S. Baba, L. Chen, Z.-J. Liu, B.-C. Wang, M. Nishida, A. Ebihara, A. Shinkai, S. Kuramitsu, Y. Shiro, K. Sekar and S. Yokoyama. Acta Cryst. (2007). F63, 27-29.

4. Crystal-contact engineering to obtain a crystal form of the Kelch domain of human Keap1 suitable for ligand-soaking experiments. Stefan Horer, Dirk Reinert, Katja Ostmann, Yvette Hoevels and Herbert Nar. Acta Cryst. (2013). F69, 592-596.

SaltRx 1 • SaltRx 2 • SaltRx HT SaltRx 1 • SaltRx 2 • SaltRx HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Silver Bullets蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Silver Bullets
Silver Bullets

Silver Bullets

Silver Bullets

Silver Bullets

Silver Bullets

Products > Optimization Screens > Silver Bullets > Silver Bullets

Silver Bullets

Applications

  • Additive screen for the optimization of protein crystals
  • For use with soluble proteins and membrane proteins
  • Additive screen to discover different crystal forms
  • Secondary or orthogonal crystallization screen when traditional screens are not successful
  • Additive screen for the optimization of protein solubility and stability

Features

  • Developed at Hampton Research
  • Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization
    • Small organic salts and organic acids
    • Biologically active small molecules
    • Amino acids & peptides
    • Macromolecular digests
    • C0-factors & ligands
    • Biochemical pathway intermediates
    • Nucleotides, pharmacaphores, & carbohydrates

Description

Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3

Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3

X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.

Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests

The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:
•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal

The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ďż˝C, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.

Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.

Silver Bullets reagent volume is 0.5 ml (each well).

ThermoFluor is a registered trademark of Johnson & Johnson.

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Silver Bullets

CAT NO

HR2-096

NAME

Silver Bullets

DESCRIPTION

0.5 ml, Deep Well block format

PRICE

$495.00

cart quote

Support Material(s)

Silver Bullets HR2-096 Silver Bullets DocumentsSilver Bullets HR2-096 Silver Bullets SDSSilver Bullets Silver Bullets SpecificationsSilver Bullets Silver Bullets Reagents Comparison

Related Item(S)

  • Individual Silver Bullets Reagents

References

1. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology 156 (2006) 387-406.

2. A novel strategy for the crystallization of proteins: X-ray diffraction validation. Steven B. Larson, John S. Day, Robert Cudney, and Alexander McPherson. Acta Cryst. (2007) D63, 310-318.

3. Development of an alternative approach to protein crystallization. Alexander McPherson, Chieniang Nguyen, Steven B. Larson, John S. Day and Bob Cudney. Journal of Structural and Functional Genomics, DOI 10.1007/s10969-007-9034-3, November 2007

4. Development of an alternative approach to protein crystallization. McPherson, Alexander; Nguyen, Chieniang; Larson, Steven B; Day, John S; Cudney, Bob. J Struct Funct Genomics, Volume 8, Number 4, December 2007, 193-198.

5. Progress in the Development of an Alternative Approach to Macromolecular Crystallization. S. B. Larson,J. S. Day, C. Nguyen, R. Cudney, and A. McPherson. Crystal Growth & Design 2008 Volume 8, No. 8 3038-3052.

6. High-resolution structure of proteinase K cocrystallized with digalacturonic acid. S. B. Larson, J. S. Day, C. Nguyen, R. Cudney and A. McPherson. Acta Cryst. (2009). F65, 192-198.

7. Structure of pig heart citrate synthase at 1.78 Angstrom resolution. Steven B. Larson, John S. Day, Chieugiang Nguyen, Robert Cudney and Alexander McPherson. Acta Cryst. (2009). F65, 430-434.

8. Crystallization and preliminary X-ray diffraction analysis of the dimerization domain of the tumour suppressor ING4. Simone Culurgioni et al, Acta Cryst. (2010). F66, 567-570.

Silver Bullets Silver Bullets

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Slice pH蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Slice pH
Slice pH

Slice pH

Slice pH

Slice pH

Slice pH

Products > Optimization Screens > Slice pH > Slice pH

Slice pH

Applications

  • Solubility screen, stability screen, crystallization screen
  • Preformulation screening
  • Sample buffer optimization for crystallization, cryo-electron microscopy, and NMR

Features

  • 96 pH titrated high purity 1.0 M buffer reagents
  • Vapor diffusion assay for solubility, stability & crystallization
  • pH 3.5 to 9.6 in 0.1 pH increments
  • Evaluate 20 different buffers
  • Use with ThermoFluor, Filter Plate, Dynamic Light Scattering, SEC, Native Gel, Western, Dot Blot/ELISA diagnostic assays to optimize sample solubility and stability

Description

Slice pH is a solubility screen, a stability screen, and a crystallization screen.

pH is an effective solubility, stability and crystallization variable because most proteins demonstrate pH dependent solubility minima and will solubilize, precipitate, or crystallize at particular pH values or in the presence of specific buffers. The solubility minima may correspond with the isoelectric point (pI) of the protein, but this is not always the case. The solubility minima and maxima is often complex and may depend on other chemical and physical variables in the crystallization experiment.

Using Slice pH one isolates pH, buffer type and relative supersaturation from other chemical and physical variables and to screen the effect that pH and buffer type have on the solubility, stability, homogeneity, monodispersity and crystallization of the sample. Varying the pH can alter the protonation state and charge of amino acid residues in the protein, generating different species of the protein for solubility and crystallization screening. The change in pH can have a dramatic effect on inter and intramolecular contacts in the protein and can manipulate how the protein interacts with itself, the surrounding solvent and chemicals in the drop. By screening buffer type and pH in an environment of increasing relative supersaturation, Slice pH simultaneously delivers as a solubility and a crystallization screen for proteins. After the screen, and once the appropriate sample buffer and pH are identified, the sample can be exchanged into the identified buffer and pH for optimal solubility and stability. From this point, the sample in the optimized buffer reagent can be used for crystallization trials or other assays. Further, crystallization screening and optimization experiments may be more appropriately focused on the optimal pH range and buffer type.

The Slice pH kit contains 500 microliters of 96 reagents in a V-Bottom (conical bottom) 96 Deep Well polypropylene microplate, user guide, formulations and a sealing film.

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Slice pH
Slice pH

CAT NO

HR2-070

NAME

Slice pH

DESCRIPTION

0.5 ml, Deep Well block format

PRICE

$301.00

cart quote

Support Material(s)

Slice pH HR2-070 Slice pH DocumentsSlice pH HR2-070 Slice pH SDSSlice pH Slice pH Formulation & Scoring Data

Related Item(S)

  • Individual Slice pH Reagents

References

1. Preparation and analysis of protein crystals. Alexander McPherson. 1982 John Wiley and Sons, Inc.

2. Increasing the size of microcrystals by fine sampling of pH limits. Alexander McPherson. J. Appl. Cryst. (1995). 28, 362-365.

3. Protein crystallization techniques, strategies and tips. Edited by Terese Bergfors. 1999. International University Line.

4. Crystallization of Biological Macromolecules. Alexander McPherson. 1991. Cold Spring Harbor Laboratory Press.

5. Current approaches to macromolecular crystallization (review). Alexander McPherson. Eur. J. Biochem. 189, 1-23 (1990).

6. Protein Isoelectric Point as a Predictor for Increased Crystallization screening efficiency. Katherine A. Kantardjieff and Bernhard Rupp. Bioinformatics (2004) 20.

7. A protein crystallization strategy using automated grid searches on successively finer grid screens. Patricia C. Weber. Methods: A Companion to Methods in Enzymology. Vol. 1, No. 1, August, pp. 31-37, 1990.

8. Two approaches to the rapid screening of crystallization conditions. Alexander McPherson. Journal of Crystal Growth 122 (1992) 161-167.

9. Optimization of buffer solutions for protein crystallization. R. A. Gosavi, T. C. Mueser and C. A. Schall. Acta Cryst. (2008). D64, 506-514.

10. Buffer Solutions The Basics. R.J. Beynon and J.S. Easterby. 1996. IRL Press.

11. Thermofluor-based optimization strategy for the stabilization and crystallization of Campylobacter jejuni desulforubrerythrin. Santos SP, Bandeiras TM, Pinto AF, Teixeira M, Carrondo MA, Romão CV. Protein Expr Purif. 2012 Feb;81(2):193-200. doi: 10.1016/j.pep.2011.10.001. Epub 2011 Oct 24.

Slice pH Slice pH

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Solubility & Stability Screen蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Solubility & Stability Screen
Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen

Products > Optimization Screens > Solubility & Stability Screen > Solubility & Stability Screen

Solubility & Stability Screen

Applications

  • A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
  • Preformulation screening
  • Sample buffer optimization for crystallization, cryo-electron microscopy, and NMR

Features

  • Compatible with Thermofluor, Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
  • 96 sterile filtered reagents
  • 17 classes of reagents and excipients
  • Highly concentrated (10x) reagent formulation
  • Developed at Hampton Research

Description

Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.

The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.

Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11 The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.

Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12

Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.

It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15 Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11

The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.

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Solubility & Stability Screen
Solubility & Stability Screen
Solubility & Stability Screen

CAT NO

HR2-072

NAME

Solubility & Stability Screen

DESCRIPTION

0.5 ml, Deep Well block format

PRICE

$301.00

cart quote

Support Material(s)

Solubility & Stability Screen HR2-072 Solubility & Stability Screen DocumentsSolubility & Stability Screen HR2-072 Solubility & Stability Screen SDSSolubility & Stability Screen Solubility & Stability Screen Formulation and Scoring Data

Related Item(S)

  • Individual Solubility & Stability Reagents

References

1. Preparation and analysis of protein crystals. Alexander McPherson. 1982 John Wiley and Sons, Inc.

2. A straight-forward method of optimizing protein solubility for NMR. Peter W.A. Howe, Journal of Biomolecular NMR 30: 283-286, 2004.

3. Rapid determination of protein solubility and stability conditions for NMR studies using incomplete factorial design. Demene, H. et al. Journal of Biomolecular NMR (2006) 34: 137-151.

4. Dissolution of protein aggregation by small amine compounds. Takagai et al. Science and Technology of Advanced Materials 4 (2003) 55-59.

5. Detection and prevention of protein aggregation before, during, and after purification. Sarah E. Bondos and Alicia Bicknell. Analytical Biochemistry 316 (2003) 223-231.

6. Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics. Sun et al. Anal Bioanal Chem (2010) DOI 10.1007/s00216-010-4381-5.

7. Stabilization of proteins by low molecular weight multi-ions. Middaugh et al. Journal of Pharmaceutical Sciences, Vol. 91, No. 10, October 2002.

8. Inhibition of aggregation side reactions during in vitro protein folding. Eliana De Bernardez Clark, Elisabeth Schwarz, Rainer Rudolph. Methods in Enzymology Volume 309 page 228 (1991).

9. Refolding a glutamine synthetase truncation mutant in vitro: Identifying superior conditions using a combination of chaperonins and osmolytes. Paul A. Voziyan, Lalita Jadhav, Mark T. Fisher. Journal of Pharmaceutical Science Volume 89 page 1036 (2000).

10. Chemical Chaperones Regulate Molecular Chaperones in Vitro and in Cells under Combined Salt and Heat Stresses. Goloubinoff et al. J. Biol. Chem., Vol. 276, Issue 43, 39586-39591, October 26, 2001.

11. Effect of additives on protein aggregation. Shiraki et al. Current Pharmaceutical Biotechnology, 2009, 10, 400-407.

12. A thermal stability assay can help to estimate the crystallization likelihood of biological samples. Marquez et al. Acta Cryst. (2011). D67, 915-919.

13. The combined use of the Thermofluor assay and ThermoQ analytical software for the determination of protein stability and buffer optimization as an aid in protein crystallization. Kevin Phillips and Andres Hernandez de la Pena. Current Protocols in Molecular Biology 10.28.1-10.28.15 April 2011.

14. Thermofluor-based optimization strategy for the stabilization and crystallization of Campylobacter jejuni desulforubrerythrin. Romao et al. Protein Expression and Purification 81 (2012) 193-200.

15. Optimization of protein solubility and stability for protein nuclear magnetic resonance. Bagby S, Tong KI, Ikura, M. Methods Enzymol. 2001;339:20-41.

16. High-Density Miniaturized Thermal Shift Assays as a General Strategy for Drug Discovery. Pantoliano et al, Journal of Biomolecular Screening, Volume 6, Number 6, 2001.

17. Thermofluor-based high-throughput stability optimization of proteins for structural studies. Ericsson, U.B. et al, Anal Biochem. 2006 Oct 15;357(2):289-98. Epub 2006 Aug 10.

18. Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination. Vedadi, M. et al, Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15835-40. Epub 2006 Oct 11.

19. Comparison of fluorescence and light scattering based methods to assess formation and stability of protein-protein complexes. J. Kopec, G. Schneider. Journal of Structural Biology 175 (2011) 216-223.

Solubility & Stability Screen Solubility & Stability Screen

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Solubility & Stability Screen 2蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Solubility & Stability Screen 2
Solubility & Stability Screen 2

Solubility & Stability Screen 2

Solubility & Stability Screen 2

Solubility & Stability Screen 2

Solubility & Stability Screen 2

Products > Optimization Screens > Solubility & Stability Screen 2 > Solubility & Stability Screen 2

Solubility & Stability Screen 2

Applications

  • Solubility & Stability Screen 2 is designed to assist in the identification of optimal buffer, pH and ionic strength formulations that promote protein solubility and stability
  • Sample buffer optimization for crystallization, cryo-electron microscopy, and NMR

Features

  • Compatible with ThermoFluor (Differential Scanning Fluorimetry, DSF, Protein Thermal Shift) and Dynamic Light Scattering assays
  • Formulation of 12 unique buffers, pH 4.5 – 9.5, versus 8 levels of ionic strength
  • Evaluate varying ionic strength in the presence and absence of buffer
  • Evaluate varying buffer type and pH in different levels of ionic strength
  • 96 sterile filtered reagents
  • Concentrated reagent formulation
  • Developed at Hampton Research

Description

Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in diagnostics and pharmaceuticals, structural biology and crystallization.

The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.

Protein solubility and stability is affected by many different chemical factors including pH, buffer type, ionic strength and protein specific additives. pH, buffer type and ionic strength are dominant protein solubility and stability variables that can be evaluated and optimized using the Solubility and Stability Screen 2.

No clear correlation between intrinsic properties of proteins and solubility and stability exist, so systematic screening can help to identify optimal sample buffer conditions. Protein solubility and stability screening, performed using Dynamic Light Scattering and ThermoFluor assays together with the Solubility and Stability Screens and Slice pH can be an extremely data rich, informative, efficient, and cost-effective method for the identification of sample buffer conditions that maximally stabilize a protein for protein purification, formulation, crystallization, and functional characterization.

Dynamic Light Scattering (DLS) is an established technique for determining the size, monodispersity and polydispersity of proteins in solution. DLS with a plate reader allows a multitude of buffers, pH, ionic strength, protein specific additives and temperature to be screened. Combining Solubility & Stability Screens with DLS, particle size and size distribution, unfolding of proteins, crystallizability, thermal stability, aggregation and solubility behavior and be evaluated in a high throughput format.

The ThermoFluor assay is an established technique for assessing protein thermostability in solution. ThermoFluor allows systematic assessment of many reagents simultaneously, uses only small a small amount of protein, and is accessible to anyone with a real-time PCR instrument.

Once an optimal buffer, pH and ionic strength is identified, the sample can be exchanged into the new optimal buffer. Using this optimized sample, further ThermoFluor assays with the Solubility & Stability Screen, Silver Bullets, Silver Bullets Bio and Additive Screen can be performed to identify protein specific additives that further promote solubility and stability of the protein.

ThermoFluor® is a registered trademark of Johnson & Johnson.

Protein Thermal Shift is a trademark of ThermoFisher.

Sypro® is a registered trademark of ThermoFisher.

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Solubility & Stability Screen 2

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Solubility & Stability Screen 2
Solubility & Stability Screen 2
Solubility & Stability Screen 2
Solubility & Stability Screen 2

Solubility & Stability Screen 2

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Solubility & Stability Screen 2
Solubility & Stability Screen 2
Solubility & Stability Screen 2
Solubility & Stability Screen 2

CAT NO

HR2-413

NAME

Solubility & Stability Screen 2

DESCRIPTION

0.5 ml, Deep Well block format

PRICE

$301.00

cart quote

Support Material(s)

Solubility & Stability Screen 2 HR2-413 Solubility & Stability Screen 2 DocumentsSolubility & Stability Screen 2 HR2-413 Solubility & Stability Screen 2 SDSSolubility & Stability Screen 2 Solubility & Stability Screen 2 Formulation & Scoring Data

Related Item(S)

  • Individual Solubility & Stability 2 Reagents

References

1. A thermal stability assay can help to estimate the crystallization likelihood of biological samples. Marquez et al. Acta Cryst. (2011). D67, 915-919.

2. The combined use of the ThermoFluor assay and ThermoQ analytical software for the determination of protein stability and buffer optimization as an aid in protein crystallization. Kevin Phillips and Andres Hernandez de la Pena. Current Protocols in Molecular Biology 10.28.1-10.28.15 April 2011.

3. Thermofluor-based optimization strategy for the stabilization and crystallization of Campylobacter jejuni desulforubrerythrin. Romao et al. Protein Expression and Purification 81 (2012) 193-200.

4. Optimization of protein solubility and stability for protein nuclear magnetic resonance. Bagby S, Tong KI, Ikura, M. Methods Enzymol. 2001;339:20-41.

5. High-Density Miniaturized Thermal Shift Assays as a General Strategy for Drug Discovery. Pantoliano et al, Journal of Biomolecular Screening, Volume 6, Number 6, 2001.

6. Thermofluor-based high-throughput stability optimization of proteins for structural studies. Ericsson, U.B. et al, Anal Biochem. 2006 Oct 15;357(2):289-98. Epub 2006 Aug 10.

7. Comparison of fluorescence and light scattering based methods to assess formation and stability of protein-protein complexes. J. Kopec, G. Schneider. Journal of Structural Biology 175 (2011) 216-223.

8. Enhancing recombinant protein quality and yield by protein stability profiling. Mezzasalma TM, Kranz JK, Chan W, Struble GT, Schalk-Hihi C, Deckman IC, Springer BA, Todd MJ. J Biomol Screen. 2007 Apr;12(3):418-28.

9. Optimization of protein purification and characterization using ThermoFluor screens. Boivin S, Kozak S, Meijers R. Protein Expr Purif. 2013 Oct;91(2):192-206. doi: 10.1016/j.pep.2013.08.002. Epub 2013 Aug 12.

10. The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability. Frank H Niesen, Helena Berglund & Masoud Vedadi. Nat Protoc. 2007;2(9):2212-21.

11. Use of dynamic light scattering to assess crystallizability of macromolecules and macromolecular assemblies. Ferre-D′Amare AR1, Burley SK. Structure. 1994 May 15;2(5):357-9.

12. Crystallizing proteins – a rational approach? D′Arcy A. Acta Crystallogr D Biol Crystallogr. 1994 Jul 1;50(Pt 4):469-71.

13. Protein Crystallization. Techniques, Strategies, and Tips. A Laboratory Manual. Edited by Terese M. Bergfors. International University Line. 1999; 27-38.

Solubility & Stability Screen 2 Solubility & Stability Screen 2

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

  • Products
  • |

  • Gallery
  • |

  • My Account
  • |

  • Contact Us
  • |

  • Quick Order
  • Support
  • Privacy Policy
  • |

  • Terms and Conditions
  • Products
  • |

  • Gallery
  • |

  • My Account
  • |

  • Support
  • |

  • Contact Us
  • |

  • Quick Order
  • |

  • Privacy Policy
  • |

  • Terms and Conditions
© 2022 HAMPTON RESEARCH CORP.


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Individual Detergent Screen Reagents蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Individual Detergent Screen Reagents
Individual Detergent Screen Reagents

Individual Detergent Screen Reagents

Individual Detergent Screen Reagents

Individual Detergent Screen Reagents

Individual Detergent Screen Reagents

Products > Custom Shop Crystallization Reagents > Detergent Screen > Individual Detergent Screen Reagents

Individual Detergent Screen Reagents

Applications

  • Individual Detergent Screen reagents for screening and optimization

Features

  • 0.5 ml volume in a sterile polypropylene tune with o-ring seal. 
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

HR2-407-** 
** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-407-01

Individual reagents from the new, revised formulation of HR2-407 Detergent Screen supplied in a volume of 0.5 milliliter in a sterile polypropylene tube with o-ring seal. All detergents formulated in deionized, sterile filtered Type 1 Laboratory Water. Container is purged with argon prior to closure.



Individual Detergent Screen Reagents

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Individual Detergent Screen Reagents

Individual Detergent Screen Reagents

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Individual Detergent Screen Reagents

CAT NO

HR2-407-01

NAME

Detergent Screen 01 (A01) 0.5 mL

DESCRIPTION

1.0 mM 14:0 Lyso PG

PRICE

$84.00

cart quote

CAT NO

HR2-407-02

NAME

Detergent Screen 02 (A02) 0.5 mL

DESCRIPTION

6.0 mM 16:0 Lyso PG

PRICE

$84.00

cart quote

CAT NO

HR2-407-03

NAME

Detergent Screen 03 (A03) 0.5 mL

DESCRIPTION

5.0 mM 18:0 Lyso PG

PRICE

$84.00

cart quote

CAT NO

HR2-407-04

NAME

Detergent Screen 04 (A04) 0.5 mL

DESCRIPTION

5.0 mM 18:1 Lyso PG

PRICE

$84.00

cart quote

CAT NO

HR2-407-05

NAME

Detergent Screen 05 (A05) 0.5 mL

DESCRIPTION

10.0 mM CTAB

PRICE

$84.00

cart quote

CAT NO

HR2-407-06

NAME

Detergent Screen 06 (A06) 0.5 mL

DESCRIPTION

1% w/v BAM

PRICE

$84.00

cart quote

CAT NO

HR2-407-07

NAME

Detergent Screen 07 (A07) 0.5 mL

DESCRIPTION

1.0 mM Dodecyltrimethylammonium chloride

PRICE

$84.00

cart quote

CAT NO

HR2-407-08

NAME

Detergent Screen 08 (A08) 0.5 mL

DESCRIPTION

150.0 mM Sodium cholate hydrate

PRICE

$84.00

cart quote

CAT NO

HR2-407-09

NAME

Detergent Screen 09 (A09) 0.5 mL

DESCRIPTION

60.0 mM Deoxycholic acid sodium salt

PRICE

$84.00

cart quote

CAT NO

HR2-407-10

NAME

Detergent Screen 10 (A10) 0.5 mL

DESCRIPTION

5.0 mM MSDH

PRICE

$84.00

cart quote

CAT NO

HR2-407-11

NAME

Detergent Screen 11 (A11) 0.5 mL

DESCRIPTION

146.0 mM N-Lauroylsarcosine sodium salt

PRICE

$84.00

cart quote

CAT NO

HR2-407-12

NAME

Detergent Screen 12 (A12) 0.5 mL

DESCRIPTION

100.0 mM Sodium dodecyl sulfate

PRICE

$84.00

cart quote

CAT NO

HR2-407-13

NAME

Detergent Screen 13 (B01) 0.5 mL

DESCRIPTION

100.0 mM Lithium dodecyl sulfate

PRICE

$84.00

cart quote

CAT NO

HR2-407-14

NAME

Detergent Screen 14 (B02) 0.5 mL

DESCRIPTION

400.0 mM APO 8

PRICE

$84.00

cart quote

CAT NO

HR2-407-15

NAME

Detergent Screen 15 (B03) 0.5 mL

DESCRIPTION

5.0 mM APO 9

PRICE

$84.00

cart quote

CAT NO

HR2-407-16

NAME

Detergent Screen 16 (B04) 0.5 mL

DESCRIPTION

47.0 mM APO 10

PRICE

$84.00

cart quote

CAT NO

HR2-407-17

NAME

Detergent Screen 17 (B05) 0.5 mL

DESCRIPTION

5.0 mM APO 11

PRICE

$84.00

cart quote

CAT NO

HR2-407-18

NAME

Detergent Screen 18 (B06) 0.5 mL

DESCRIPTION

5.7 mM APO 12

PRICE

$84.00

cart quote

CAT NO

HR2-407-19

NAME

Detergent Screen 19 (B07) 0.5 mL

DESCRIPTION

80.0 mM Tetraethylene glycol monooctyl ether

PRICE

$84.00

cart quote

CAT NO

HR2-407-20

NAME

Detergent Screen 20 (B08) 0.5 mL

DESCRIPTION

71.0 mM Pentaethylene glycol monooctyl ether

PRICE

$84.00

cart quote

CAT NO

HR2-407-21

NAME

Detergent Screen 21 (B09) 0.5 mL

DESCRIPTION

1.1 mM C12E8

PRICE

$84.00

cart quote

CAT NO

HR2-407-22

NAME

Detergent Screen 22 (B10) 0.5 mL

DESCRIPTION

0.8 mM C12E9

PRICE

$84.00

cart quote

CAT NO

HR2-407-23

NAME

Detergent Screen 23 (B11) 0.5 mL

DESCRIPTION

1% v/v Brij 35

PRICE

$84.00

cart quote

CAT NO

HR2-407-24

NAME

Detergent Screen 24 (B12) 0.5 mL

DESCRIPTION

1% v/v Brij 56

PRICE

$84.00

cart quote

CAT NO

HR2-407-25

NAME

Detergent Screen 25 (C01) 0.5 mL

DESCRIPTION

1% v/v Brij 58

PRICE

$84.00

cart quote

CAT NO

HR2-407-26

NAME

Detergent Screen 26 (C02) 0.5 mL

DESCRIPTION

1% v/v Genapol X-080

PRICE

$84.00

cart quote

CAT NO

HR2-407-27

NAME

Detergent Screen 27 (C03) 0.5 mL

DESCRIPTION

1.8 mM Facade-EM

PRICE

$84.00

cart quote

CAT NO

HR2-407-28

NAME

Detergent Screen 28 (C04) 0.5 mL

DESCRIPTION

9.7 mM Facade-EPC

PRICE

$84.00

cart quote

CAT NO

HR2-407-29

NAME

Detergent Screen 29 (C05) 0.5 mL

DESCRIPTION

1.9 mM Facade-TEG

PRICE

$84.00

cart quote

CAT NO

HR2-407-30

NAME

Detergent Screen 30 (C06) 0.5 mL

DESCRIPTION

1.9 mM Facade-TEM

PRICE

$84.00

cart quote

CAT NO

HR2-407-31

NAME

Detergent Screen 31 (C07) 0.5 mL

DESCRIPTION

0.13 mM Facade-TFA1

PRICE

$84.00

cart quote

CAT NO

HR2-407-32

NAME

Detergent Screen 32 (C08) 0.5 mL

DESCRIPTION

790.0 mM MEGA-8

PRICE

$84.00

cart quote

CAT NO

HR2-407-33

NAME

Detergent Screen 33 (C09) 0.5 mL

DESCRIPTION

250.0 mM MEGA-9

PRICE

$84.00

cart quote

CAT NO

HR2-407-34

NAME

Detergent Screen 34 (C10) 0.5 mL

DESCRIPTION

70.0 mM MEGA-10

PRICE

$84.00

cart quote

CAT NO

HR2-407-35

NAME

Detergent Screen 35 (C11) 0.5 mL

DESCRIPTION

3.7 mM Mal(11.1)

PRICE

$84.00

cart quote

CAT NO

HR2-407-36

NAME

Detergent Screen 36 (C12) 0.5 mL

DESCRIPTION

1.1 mM Mal(11.2)

PRICE

$84.00

cart quote

CAT NO

HR2-407-37

NAME

Detergent Screen 37 (D01) 0.5 mL

DESCRIPTION

105.0 mM N,N-Dimethyldecylamine-N-oxide

PRICE

$84.00

cart quote

CAT NO

HR2-407-38

NAME

Detergent Screen 38 (D02) 0.5 mL

DESCRIPTION

18.0 mM n-Decyl-ß-D-maltoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-39

NAME

Detergent Screen 39 (D03) 0.5 mL

DESCRIPTION

2.0 mM n-Dodecyl-ß-D-maltoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-40

NAME

Detergent Screen 40 (D04) 0.5 mL

DESCRIPTION

1.0 mM n-Hexadecyl-ß-D-maltoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-41

NAME

Detergent Screen 41 (D05) 0.5 mL

DESCRIPTION

1.0 mM n-Tetradecyl-ß-D-maltoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-42

NAME

Detergent Screen 42 (D06) 0.5 mL

DESCRIPTION

1.0 mM Tridecyl-ß-D-maltoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-43

NAME

Detergent Screen 43 (D07) 0.5 mL

DESCRIPTION

5.9 mM Undecyl-ß-D-maltoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-44

NAME

Detergent Screen 44 (D08) 0.5 mL

DESCRIPTION

195.0 mM Methyl-6-O-(N-heptylcarbamoyl)-a-D-glucopyranoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-45

NAME

Detergent Screen 45 (D09) 0.5 mL

DESCRIPTION

5.0 mM IPTG

PRICE

$84.00

cart quote

CAT NO

HR2-407-46

NAME

Detergent Screen 46 (D10) 0.5 mL

DESCRIPTION

25.0 mM n-Decanoylsucrose

PRICE

$84.00

cart quote

CAT NO

HR2-407-47

NAME

Detergent Screen 47 (D11) 0.5 mL

DESCRIPTION

65.0 mM n-Nonyl-ß-D-glucoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-48

NAME

Detergent Screen 48 (D12) 0.5 mL

DESCRIPTION

200.0 mM n-Octyl-ß-D-glucoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-49

NAME

Detergent Screen 49 (E01) 0.5 mL

DESCRIPTION

90.0 mM n-Octyl-ß-D-thioglucopyranoside

PRICE

$84.00

cart quote

CAT NO

HR2-407-50

NAME

Detergent Screen 50 (E02) 0.5 mL

DESCRIPTION

10% w/v Pluronic F-68

PRICE

$84.00

cart quote

CAT NO

HR2-407-51

NAME

Detergent Screen 51 (E03) 0.5 mL

DESCRIPTION

10% w/v Pluronic F-127

PRICE

$84.00

cart quote

CAT NO

HR2-407-52

NAME

Detergent Screen 52 (E04) 0.5 mL

DESCRIPTION

3.0 mM Sucrose monolaureate

PRICE

$84.00

cart quote

CAT NO

HR2-407-53

NAME

Detergent Screen 53 (E05) 0.5 mL

DESCRIPTION

0.9 mM Thesit

PRICE

$84.00

cart quote

CAT NO

HR2-407-54

NAME

Detergent Screen 54 (E06) 0.5 mL

DESCRIPTION

1% v/v Triton X-100

PRICE

$84.00

cart quote

CAT NO

HR2-407-55

NAME

Detergent Screen 55 (E07) 0.5 mL

DESCRIPTION

1% v/v Triton X-114

PRICE

$84.00

cart quote

CAT NO

HR2-407-56

NAME

Detergent Screen 56 (E08) 0.5 mL

DESCRIPTION

1% v/v Nonylphenyl polyethylene glycol

PRICE

$84.00

cart quote

CAT NO

HR2-407-57

NAME

Detergent Screen 57 (E09) 0.5 mL

DESCRIPTION

1% v/v Tween 20

PRICE

$84.00

cart quote

CAT NO

HR2-407-58

NAME

Detergent Screen 58 (E10) 0.5 mL

DESCRIPTION

1% v/v Tween 80

PRICE

$84.00

cart quote

CAT NO

HR2-407-59

NAME

Detergent Screen 59 (E11) 0.5 mL

DESCRIPTION

0.18 mM GDN

PRICE

$84.00

cart quote

CAT NO

HR2-407-60

NAME

Detergent Screen 60 (E12) 0.5 mL

DESCRIPTION

80.0 mM CHAPS

PRICE

$84.00

cart quote

CAT NO

HR2-407-61

NAME

Detergent Screen 61 (F01) 0.5 mL

DESCRIPTION

80.0 mM CHAPSO

PRICE

$84.00

cart quote

CAT NO

HR2-407-62

NAME

Detergent Screen 62 (F02) 0.5 mL

DESCRIPTION

29.0 mM BIG CHAP

PRICE

$84.00

cart quote

CAT NO

HR2-407-63

NAME

Detergent Screen 63 (F03) 0.5 mL

DESCRIPTION

14.0 mM Deoxy Big Chap

PRICE

$84.00

cart quote

CAT NO

HR2-407-64

NAME

Detergent Screen 64 (F04) 0.5 mL

DESCRIPTION

43.0 mM DDMAB

PRICE

$84.00

cart quote

CAT NO

HR2-407-65

NAME

Detergent Screen 65 (F05) 0.5 mL

DESCRIPTION

20.0 mM LDAO

PRICE

$84.00

cart quote

CAT NO

HR2-407-66

NAME

Detergent Screen 66 (F06) 0.5 mL

DESCRIPTION

10% w/v Sulfobetaine 8

PRICE

$84.00

cart quote

CAT NO

HR2-407-67

NAME

Detergent Screen 67 (F07) 0.5 mL

DESCRIPTION

400.0 mM Sulfobetaine 10

PRICE

$84.00

cart quote

CAT NO

HR2-407-68

NAME

Detergent Screen 68 (F08) 0.5 mL

DESCRIPTION

40.0 mM Sulfobetaine 12

PRICE

$84.00

cart quote

CAT NO

HR2-407-69

NAME

Detergent Screen 69 (F09) 0.5 mL

DESCRIPTION

4.0 mM Sulfobetaine 14

PRICE

$84.00

cart quote

CAT NO

HR2-407-70

NAME

Detergent Screen 70 (F10) 0.5 mL

DESCRIPTION

0.6 mM Sulfobetaine 16

PRICE

$84.00

cart quote

CAT NO

HR2-407-71

NAME

Detergent Screen 71 (F11) 0.5 mL

DESCRIPTION

150.0 mM 06:0 PC (DHPC)

PRICE

$84.00

cart quote

CAT NO

HR2-407-72

NAME

Detergent Screen 72 (F12) 0.5 mL

DESCRIPTION

14.0 mM 07:0 PC (DHPC)

PRICE

$84.00

cart quote

CAT NO

HR2-407-73

NAME

Detergent Screen 73 (G01) 0.5 mL

DESCRIPTION

5.0 mM 06:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-74

NAME

Detergent Screen 74 (G02) 0.5 mL

DESCRIPTION

5.0 mM 07:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-75

NAME

Detergent Screen 75 (G03) 0.5 mL

DESCRIPTION

60.0 mM 08:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-76

NAME

Detergent Screen 76 (G04) 0.5 mL

DESCRIPTION

5.0 mM 09:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-77

NAME

Detergent Screen 77 (G05) 0.5 mL

DESCRIPTION

80.0 mM 10:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-78

NAME

Detergent Screen 78 (G06) 0.5 mL

DESCRIPTION

5.0 mM 11:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-79

NAME

Detergent Screen 79 (G07) 0.5 mL

DESCRIPTION

9.0 mM 12:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-80

NAME

Detergent Screen 80 (G08) 0.5 mL

DESCRIPTION

5.0 mM 13:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-81

NAME

Detergent Screen 81 (G09) 0.5 mL

DESCRIPTION

1.0 mM 14:0 Lyso PC

PRICE

$84.00

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CAT NO

HR2-407-82

NAME

Detergent Screen 82 (G10) 0.5 mL

DESCRIPTION

5.0 mM 15:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-83

NAME

Detergent Screen 83 (G11) 0.5 mL

DESCRIPTION

1.0 mM 16:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-84

NAME

Detergent Screen 84 (G12) 0.5 mL

DESCRIPTION

1.0 mM 17:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-85

NAME

Detergent Screen 85 (H01) 0.5 mL

DESCRIPTION

1.0 mM 18:0 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-86

NAME

Detergent Screen 86 (H02) 0.5 mL

DESCRIPTION

1.0 mM 18:1 Lyso PC

PRICE

$84.00

cart quote

CAT NO

HR2-407-87

NAME

Detergent Screen 87 (H03) 0.5 mL

DESCRIPTION

110.0 mM MAPCHO-10

PRICE

$84.00

cart quote

CAT NO

HR2-407-88

NAME

Detergent Screen 88 (H04) 0.5 mL

DESCRIPTION

11.0 mM MAPCHO-12

PRICE

$84.00

cart quote

CAT NO

HR2-407-89

NAME

Detergent Screen 89 (H05) 0.5 mL

DESCRIPTION

1.2 mM MAPCHO-14

PRICE

$84.00

cart quote

CAT NO

HR2-407-90

NAME

Detergent Screen 90 (H06) 0.5 mL

DESCRIPTION

1.0 mM MAPCHO-16

PRICE

$84.00

cart quote

CAT NO

HR2-407-91

NAME

Detergent Screen 91 (H07) 0.5 mL

DESCRIPTION

500.0 mM NDSB-195

PRICE

$84.00

cart quote

CAT NO

HR2-407-92

NAME

Detergent Screen 92 (H08) 0.5 mL

DESCRIPTION

500.0 mM NDSB-201

PRICE

$84.00

cart quote

CAT NO

HR2-407-93

NAME

Detergent Screen 93 (H09) 0.5 mL

DESCRIPTION

500.0 mM NDSB-211

PRICE

$84.00

cart quote

CAT NO

HR2-407-94

NAME

Detergent Screen 94 (H10) 0.5 mL

DESCRIPTION

500.0 mM NDSB-221

PRICE

$84.00

cart quote

CAT NO

HR2-407-95

NAME

Detergent Screen 95 (H11) 0.5 mL

DESCRIPTION

500.0 mM NDSB-256

PRICE

$84.00

cart quote

CAT NO

HR2-407-96

NAME

Detergent Screen 96 (H12) 0.5 mL

DESCRIPTION

500.0 mM NDSB-256 4T

PRICE

$84.00

cart quote

Support Material(s)

Individual Detergent Screen Reagents HR2-407-** Individual Detergent Screen Reagents SDS

Related Item(S)

  • Detergent Screen

Individual Detergent Screen Reagents Individual Detergent Screen Reagents

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Crystal Crusher蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Crystal Crusher
Crystal Crusher

Crystal Crusher

Crystal Crusher

Crystal Crusher

Crystal Crusher

Products > Tools & Seeding > Crystal Crusher > Crystal Crusher

Crystal Crusher

Applications

  • Crush macro crystals to create micro crystals

Features

  • Solid, borosilicate glass tool and handle
  • Smooth, hemispherical tip, will not damage crystallization plate
  • Prepare micro crystals for seed stock for Microseed Matrix Screening(MMS)

Description

The Crystal Crusher is designed to crush macro crystals into micro crystals for seeding and other applications. The hemispherical end of the tool fits round, concave bottomed sitting drop crystallization plates for efficient crystal crushing. The Crystal Crusher is molded from solid, borosilicate glass. One end of the tool features a larger diameter, cylindrical handle, while the opposite end features a smooth, hemispherical end for crushing crystals without damaging crystallization plates.

Overall length: ~ 43 mm
Diameter of handle: ~ 3 mm
Diameter of hemispherical end: ~ 1 mm

Five Crystal Crushers per pack.

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Crystal Crusher

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Crystal Crusher
Crystal Crusher
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Crystal Crusher
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Crystal Crusher

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Crystal Crusher
Crystal Crusher
Crystal Crusher
Crystal Crusher
Crystal Crusher

CAT NO

HR4-216

NAME

Crystal Crusher

DESCRIPTION

5 pack

PRICE

$25.00

cart quote

Support Material(s)

Crystal Crusher HR4-216 Crystal Crusher User Guide

Related Item(S)

  • Seed Bead™ Kits
  • Crystal Clear Sealing Tape
  • X-Acto Gripster Knife

References

1. Microseed matrix screening for optimization in protein crystallization: what have we learned? D’Arcy A, Bergfors T, Cowan-Jacob SW, Marsh M. Acta Crystallogr F Struct Biol Commun. 2014 Sep;70 (Pt 9):1117-26. doi: 10.1107/S2053230X14015507. Epub 2014 Aug 29.

Crystal Crusher Crystal Crusher

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Sodium acetate trihydrate Buffer蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Sodium acetate trihydrate Buffer
Sodium acetate trihydrate Buffer

Sodium acetate trihydrate Buffer

Sodium acetate trihydrate Buffer

Sodium acetate trihydrate Buffer

Sodium acetate trihydrate Buffer

Products > Optimize Reagents > Optimize – Buffers > Sodium acetate trihydrate Buffer

Sodium acetate trihydrate Buffer

Applications

  • Crystallization grade Sodium acetate trihydrate buffer for formulating screens or for optimization

Features

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Formulated from Sodium acetate trihydrate

Synonyms: Acetic acid sodium salt trihydrate
C2H3NaO2 • 3H2O
CH3COONa • 3H2O
Mr 136.08
CAS Number [6131-90-4]
EC Number 204-823-8
Merck 14,8571
Beilstein Registry Number 3732037
RTECS AJ4580000
MDL Number MFCD00071557
PubChem Substance ID 24886015
Purity ≥ 99.5%
pKa = 4.8

The useful pH range of a Sodium acetate trihydrate buffer is pH 3.6 – 5.6.

Titrate HR2-569 to the desired pH using HCl
HR2-569 Measured pH Range: 8.7 – 9.3 at 25°C
HR2-569 Measured Conductivity Range: 48.0 – 51.7 mS/cm at 25°C
HR2-569 Measured Refractive Index Range: 1.34395 – 1.34415 at 20°C

HR2-789 has been titrated to pH 4.5 using HCl
HR2-789 Measured Conductivity Range: 60.8 – 67.7 mS/cm at 25°C
HR2-789 Measured Refractive Index Range: 1.34530 – 1.34579 at 20°C

HR2-731 has been titrated to pH 4.6 using HCl
HR2-731 Measured Conductivity Range: 59.3 – 64.5 mS/cm at 25°C
HR2-731 Measured Refractive Index Range: 1.34535 – 1.345745 at 20°C

Al  ≤0.0005%
As ≤0.00001%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.0005%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Ni  ≤0.0005%
Pb  ≤0.0005%
PO4 ≤0.0005%
SO4 ≤0.002%
Sr  ≤0.0005%
Zn  ≤0.0005%

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Sodium acetate trihydrate Buffer

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Sodium acetate trihydrate Buffer

Sodium acetate trihydrate Buffer

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Sodium acetate trihydrate Buffer

CAT NO

HR2-569

NAME

1.0 M Sodium acetate trihydrate

DESCRIPTION

100 mL

PRICE

$41.00

cart quote

CAT NO

HR2-789

NAME

1.0 M Sodium acetate trihydrate pH 4.5

DESCRIPTION

100 mL

PRICE

$41.00

cart quote

CAT NO

HR2-731

NAME

1.0 M Sodium acetate trihydrate pH 4.6

DESCRIPTION

100 mL

PRICE

$41.00

cart quote

Support Material(s)

Sodium acetate trihydrate Buffer HR2-569 Sodium acetate trihydrate solution SDSSodium acetate trihydrate Buffer HR2-789 Sodium acetate trihydrate pH 4.5 solution SDSSodium acetate trihydrate Buffer HR2-731 Sodium acetate trihydrate pH 4.6 solution SDSSodium acetate trihydrate Buffer Titration Table

Certificate Of Analysis

Related Item(S)

  • Hydrochloric acid
  • StockOptions Sodium Acetate Buffer Kit
  • Individual StockOptions Sodium Acetate Reagents

References

1. Crystal structure of thioltransferase at 2.2 A resolution. Katti, SK; Robbins, AH; Yang, Y; Wells, WW. Protein Sci 4, 1998- 2005, 1995.

Sodium acetate trihydrate Buffer Sodium acetate trihydrate Buffer

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Potassium bromide蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Potassium bromide
Potassium bromide

Potassium bromide

Potassium bromide

Potassium bromide

Potassium bromide

Products > Optimize Reagents > Optimize – Salts > Potassium bromide

Potassium bromide

Applications

  • Crystallization grade Potassium bromide for formulating screens or for optimization

Features

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Potassium bromide

Synonym: None
KBr Mr 119.00
CAS Number [7758-02-3]
EC Number 231-830-3
Merck 14,7618
RTECS TS7650000
MDL Number MFCD00011358
PubChem Substance ID 24854613
Purity ≥99.0%

Measured pH range: 5.6 – 9.1 at 25°C
Measured Conductivity Range: 430.8 – 440.4 mS/cm at 25°C
Measured Refractive Index Range: 1.38427 – 1.38495 at 20°C

Total Impurities ≤0.005% (insolubles)
Ba    ≤0.002%
BrO3 ≤0.001%
Ca    ≤0.002%
Cl     ≤0.2%
Fe    ≤5 ppm
IO ≤0.001%
I     ≤0.001%
Mg  ≤0.001%
Na  ≤0.02%
SO4 ≤0.005%
Heavy metals (as Pb) ≤5 ppm

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Potassium bromide

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Potassium bromide

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Potassium bromide

CAT NO

HR2-779

NAME

4.0 M Potassium bromide

DESCRIPTION

100 mL

PRICE

$101.00

cart quote

Support Material(s)

Potassium bromide HR2-779 Potassium bromide SDS

Certificate Of Analysis

References

1. Isolation of Ferritin Crystal from Rabbit Liver and Spleen. NORIKO MURAOKA, SHINJI NAKAJIMA, TAKESHI KATO, and TOSHIO SHIMADA. The Journal of Biochemistry Volume 60, Number 5 Pp. 489-495 (1966).

Potassium bromide Potassium bromide

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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12 mm Siliconized Glass Cover Slides蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

12 mm Siliconized Glass Cover Slides
12 mm Siliconized Glass Cover Slides

12 mm Siliconized Glass Cover Slides

12 mm Siliconized Glass Cover Slides

12 mm Siliconized Glass Cover Slides

12 mm Siliconized Glass Cover Slides

Products > Crystallization Plates, Hardware & Accessories > Cover Slides & Related Tools > 12 mm Siliconized Glass Cover Slides

12 mm Siliconized Glass Cover Slides

Applications

  • Siliconized glass cover slides for hanging & sitting drop crystallization
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization

Features

  • High purity of glass
  • Siliconized, hydrophobic glass surface
  • 0.22 mm & 0.96 mm glass thickness
  • 12 mm glass diameter
  • Circle
  • 0.22 mm glass thickness is UV Compatible (>85% transmission)

Description

Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution.

These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510) or Dow Corning 7 Release Compound (HR3-508) to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

Critical surface tension Untreated glass 78 dynes/cm Siliconized glass 31 dynes/cm Surface resistivity Untreated glass 1×1012 ohms Siliconized glass 1.2 x 1013 ohms Coefficient of friction static, glass slide on glass slide Untreated 0.9-1.0 Siliconized 0.2-0.3 Protein adsorption (100 hours) Untreated glass 0.13 mg/mm2 Siliconized glass 0.01-0.02 mg/mm2

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12 mm Siliconized Glass Cover Slides

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12 mm Siliconized Glass Cover Slides
12 mm Siliconized Glass Cover Slides

12 mm Siliconized Glass Cover Slides

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12 mm Siliconized Glass Cover Slides
12 mm Siliconized Glass Cover Slides

CAT NO

HR3-278T

NAME

Siliconized circle cover slides for Tecan robot

DESCRIPTION

0.5 oz pack (~240 slides)

PRICE

$54.00

cart quote

CAT NO

HR3-277

NAME

12 mm x 0.22 mm Siliconized circle cover slides

DESCRIPTION

0.5 ounce pack (~240 slides)

PRICE

$54.00

cart quote

CAT NO

HR3-279

NAME

12 mm x 0.22 mm Siliconized circle cover slides

DESCRIPTION

5.0 ounce case (~2,400 slides)

PRICE

$519.00

cart quote

CAT NO

HR8-088

NAME

12mmx0.96mmThick Siliconized circle cover slides

DESCRIPTION

1.0 ounce pack (~106 slides)

PRICE

$32.00

cart quote

CAT NO

HR8-090

NAME

12mmx0.96mm Thick Siliconized circle cover slides

DESCRIPTION

10.0 ounce case (~1,060 slides)

PRICE

$313.00

cart quote

Related Item(S)

  • VDX48 Plate with sealant

References

 

12 mm Siliconized Glass Cover Slides 12 mm Siliconized Glass Cover Slides

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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45° Angled Forcep蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

45° Angled Forcep
45° Angled Forcep

45° Angled Forcep

45° Angled Forcep

45° Angled Forcep

45° Angled Forcep

Products > Tools & Seeding > Forceps > 45° Angled Forcep

45° Angled Forcep

Applications

  • Manipulate, move and handle cover slides, cryoloops & more

Features

  • Length – 137 mm
  • Tip – 2.5 mm to point

Description

All purpose angled forcep with blunt end. Tip has serration for firm grip. Good for retrieving. 45° angle makes for easy manipulation. Especially nice for flipping cover slides. Has serrated non-slip handle.



45° Angled Forcep

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45° Angled Forcep
45° Angled Forcep
45° Angled Forcep

45° Angled Forcep

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45° Angled Forcep
45° Angled Forcep
45° Angled Forcep

CAT NO

HR4-859

NAME

45° Angled Forcep

DESCRIPTION

each

PRICE

$13.00

cart quote

45° Angled Forcep 45° Angled Forcep

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Locking Forcep蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Locking Forcep
Locking Forcep

Locking Forcep

Locking Forcep

Locking Forcep

Locking Forcep

Products > Tools & Seeding > Forceps > Locking Forcep

Locking Forcep

Applications

  • Manipulate, move and handle cover slides, cryoloops & more

Features

  • Length – 156 mm
  • Tip Width- 1.7 mm

Description

All purpose straight forcep. Tip has serrated surface for firm grip. Good for retrieving. When closed and squeezed, forcep locks to clamp and hold item in place. Lock is released by moving “lock” toward tip with thumb. Serrated, non-slip handle.



Locking Forcep

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Locking Forcep
Locking Forcep
Locking Forcep
Locking Forcep
Locking Forcep
Locking Forcep

Locking Forcep

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Locking Forcep
Locking Forcep
Locking Forcep
Locking Forcep
Locking Forcep
Locking Forcep

CAT NO

HR4-883

NAME

Locking Forcep

DESCRIPTION

each

PRICE

$16.00

cart quote

Locking Forcep Locking Forcep

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Glass Number 50 Capillaries蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Glass Number 50 Capillaries
Glass Number 50 Capillaries

Glass Number 50 Capillaries

Glass Number 50 Capillaries

Glass Number 50 Capillaries

Glass Number 50 Capillaries

Products > Capillaries, Mounts & Supplies > Capillaries > Glass Number 50 Capillaries

Glass Number 50 Capillaries

Applications

  • X-ray data collection
  • Liquid-liquid diffusion crystallization
  • Gel acupuncture crystallization

Features

  • Thin walled – 10 micron
  • Glass Number 50 (Glass 0500, borosilicate)

Description

Glass Number 50 (Glass 0500, borosilicate glass) capillaries that are extremely thin walled (approximately 10 micron wall thickness). The length of the capillary has a well defined diameter, with one end having a funnel shape and the other end closed. Glass capillaries have a wall thickness of 0.01 mm and an overall length of 80 mm +/- 5 mm. Glass capillaries are available in a wide range of outside diameters from 0.1 mm to 2.0 mm. They are designed to mount, hold, and store small molecule and biological macromolecular crystals for x-ray data collection. Capillaries can also be used for crystal density measurements and crystal growth experiments. The capillaries can be sealed tightly against moisture and gases using wax, epoxy, or other sealing materials. 

In determining what glass or quartz capillary is right for you, please refer to the “Linear Absorption Coefficient µ cm-1” table. This table indicates the amount of radiation that is absorbed by the capillary during x-ray data collection.

For 0.1 mm to 2.0 mm capillaries the open end capillary tube base size is 3.0 +/- 0.15 mm OD x 0.13 +/- 0.10 mm Wall thickness.

The Diameter is measured  about 30 to 40 mm from the closed end (measuring instrument: LaserMicrometer LS 7500) The tolerances are as follows. 

Diameter    Tolerance              Minimum Diameter    Maximum Diameter

0.1 mm       +/-0.05 mm          0.05 mm                     0.15 mm
0.2 mm       +/-0.05 mm          0.15 mm                     0.25 mm 
0.3 mm       +/-0.05 mm          0.25 mm                     0.35 mm  
0.4 mm       +/-0.05 mm          0.35 mm                     0.45 mm 
0.5 mm       +/-0.05 mm          0.45 mm                     0.55 mm 
0.6 mm       +/-0.05 mm          0.55 mm                     0.65 mm 
0.7 mm       +/-0.05 mm          0.65 mm                     0.75 mm 
0.8 mm       +/-0.05 mm          0.75 mm                     0.85 mm 
0.9 mm       +/-0.05 mm          0.85 mm                     0.95 mm 
1.0 mm       -0.05 +0.25 mm    0.95 mm                     1.25 mm 
1.5 mm       +/-0.25 mm          1.25 mm                     1.75 mm 
2.0 mm       +/-0.25 mm          1.75 mm                     2.25 mm 

Borosilicate Specifications
Glass type Borosilicate glass 3.3
Density 2.23 g/cm3
Poisson’s number 0.20
Transformation temperature Tg= 525 degrees Celsius
Annealing point approximately 560 degrees Celsius 
Softening point approximately 825 degrees Celsius 
Working point 1260 degrees Celsius 
Refractive index 1.473 at 20 degrees Celsius; 587.6 nm
Hydrolytic resistance, class 1 (DIN ISO 719) 
Acid resistance, class 1 (DIN 12 116) 
Alkalai resistance, class 2 (DIN ISO 695) 
Chemical composition SiO2 81.0% 
Na2O 3.5%
Al2O3 2.0%
K2O 0.5%
B2O3 13.0%

Capillaries have only been tested at atmospheric pressure (760 mmHg (torr), 29.92 inHg, 14.696 psi). Use at other pressures has not been tested.

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Glass Number 50 Capillaries

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Glass Number 50 Capillaries
Glass Number 50 Capillaries
Glass Number 50 Capillaries
Glass Number 50 Capillaries
Glass Number 50 Capillaries
Glass Number 50 Capillaries

Glass Number 50 Capillaries

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Glass Number 50 Capillaries
Glass Number 50 Capillaries
Glass Number 50 Capillaries
Glass Number 50 Capillaries
Glass Number 50 Capillaries
Glass Number 50 Capillaries

CAT NO

HR6-104

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 0.1 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-106

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 0.2 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-108

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 0.3 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-110

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 0.4 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-112

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 0.5 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-114

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 0.6 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-116

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 0.7 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-118

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 0.8 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-120

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 0.9 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-122

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 1.0 mm – 25 pack

PRICE

$112.00

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CAT NO

HR6-124

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 1.5 mm – 25 pack

PRICE

$128.00

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CAT NO

HR6-126

NAME

Glass Number 50 Capillary

DESCRIPTION

Size: 2.0 mm – 25 pack

PRICE

$140.00

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Support Material(s)

Glass Number 50 Capillaries Tolerances for CapillariesGlass Number 50 Capillaries Glass Number 50 Technical Data Sheet

Related Item(S)

  • Capillary Cutting Stone™
  • Duco Cement
  • Beeswax
  • Wax Pen
  • Four Color Mounting Clay
  • Adjustable Crystal Mount
  • Brass Specimen Pin

References

 

Glass Number 50 Capillaries Glass Number 50 Capillaries

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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