葡萄糖氧化酶检测试剂盒 Glucose Oxidase Assay Kit 货号:K-GLOX Megazyme中文站

葡萄糖氧化酶检测试剂盒

英文名:Glucose Oxidase Assay Kit

货号:K-GLOX

规格:200 assays (manual) / 2000 assays (microplate)

The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.

Glucose Oxidase assay kit is suitable for manual, auto-analyser and microplate formats.
 

Colourimetric method for the determination of Glucose Oxidase
in foodstuffs and fermentation products

Principle:
(glucose oxidase)
(1) D-Glucose + H2O + O2 → D-glucono-δ-lactone + H2O2

(peroxidase)
(2) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 200 assays (manual) / 2000 (microplate)
/ 1960 (auto-analyser)
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 10 U/L
Application examples:
Enzyme preparations, and other materials (e.g. biological cultures,
samples, etc.)
Method recognition: Novel method

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 12 months after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

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淀粉总量HK检测试剂盒 Total Starch HK Assay Kit 货号:K-TSHK Megazyme中文站

淀粉总量HK检测试剂盒

英文名: Total Starch HK Assay Kit

货号:K-TSHK

规格:100 assays per kit

A modification of AOAC Method 996.11 AACC Method 76-13.01 RACI Standard Method for the measurement and analysis of total starch in cereal flours and food products. This kit contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). The method has been further modified by adjusting the D-glucose determination to a hexokinase/glucose-6-phosphate dehydrogenase/NADP+ based format.

UV-method for the determination of Total Starch in grains,
animal feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C + DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(hexokinase)
(3) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size: 100 assays
Method: Spectrophotometric at 340 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard
Method No. 168), and RACI (Standard Method)

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

 

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三磷酸腺苷Adenosine 5’-triphosphate Adenosine 5′-triphosphate (ATP) 20g 货号:C-ATP-20G Megazyme中文站

三磷酸腺苷Adenosine 5’-triphosphate

英文名:Adenosine 5′-triphosphate (ATP) 20g

货号:C-ATP-20G

规格:20g

三磷酸腺苷

High purity Adenosine 5’-triphosphate for use as a cofactor for research, biochemical enzyme assays and in vitro diagnostic analysis.

Adenosine 5’-triphosphate, disodium salt (ATP, 5’-ATP, ATP-Na2). MW: 507.18 for free acid. Purity: 97.9 %; ATP content, 82.9 %; sodium content, 7.5 %; moisture content, ~ 8 %.

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磷酸葡糖异构酶[枯草杆菌] Phosphoglucose isomerase (Bacillus subtilis) 货号:E-PGIBS-5KU Megazyme中文站

磷酸葡糖异构酶[枯草杆菌]

英文名:Phosphoglucose isomerase (Bacillus subtilis)

货号:E-PGIBS-5KU

规格:5000 units

High purity Phosphoglucose isomerase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 5.3.1.9

From Bacillus subtilis. Electrophoretically homogeneous (MW 51,500). Several bands on isoelectric focusing (pI 4.8-5.0).
In 3.2 M ammonium sulphate.

Specific activity: 250 U/mg of protein (25oC, pH 7.6) or 483 U/mg of protein (40oC, pH 7.6).

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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转化酶 呋喃果糖苷酶(酵母) Invertase (fructofuranosidase) (yeast) 货号:E-INVRT Megazyme中文站

转化酶 呋喃果糖苷酶(酵母)

英文名:Invertase (fructofuranosidase) (yeast)

货号:E-INVRT

规格:100 mL; 200000 Units. 2000 U/mL

High purity Invertase (beta-Fructofuranosidase) (liquid) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.26

From yeast. Chromatographically purified. 
In 50% glycerol.

Specific activity: 300 U/mg protein (40oC, pH 4.5, 1% sucrose). 2,000 U/ml. 

Stable at 4oC for > 4 years.

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23-α-L-阿糖呋喃-木三糖(A2XX) 23-α-L-Arabinofuranosyl-xylotriose (A2XX) 货号:O-A2XX Megazyme中文站

23-α-L-阿糖呋喃-木三糖(A2XX)

英文名:23-α-L-Arabinofuranosyl-xylotriose (A2XX)

货号:O-A2XX

规格:30 mg

CAS: 152842-73-4
Molecular Formula: C20H34O17
Molecular Weight: 546.5
Purity: > 95%

High purity 23-α-L-Arabinofuranosyl-xylotriose for or use in research, biochemical enzyme assays and in vitro diagnostic analysis. It can be used as an analytical standard or as a substrate to help characterise the activities of arabinoxylan degrading enzymes including endo-xylanase, β-xylosidase and α-L-arabinofuranosidase. This compound was prepared by the controlled enzymatic hydrolysis of wheat arabinoxylan. 

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2-氯-4-硝基苯基β-纤维二糖苷 2-Chloro-4-nitrophenyl-β-cellobioside 货号:O-CPNPG2-100 Megazyme中文站

2-氯-4-硝基苯基β-纤维二糖苷

英文名:2-Chloro-4-nitrophenyl-β-cellobioside

货号:O-CPNPG2-100

规格:100 mg

CAS: 135743-28-1
Molecular Formula: C18H24CINO13
Molecular Weight: 497.8
Purity: > 98%

High purity 2-Chloro-4-nitrophenyl-β-cellobioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

A potential substrate for the measurement of cellulase (endo-1,4-β-glucanase).

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Azo-酪蛋白(磺胺染料) Azo-Casein (Sulphanilamide Dyed) 货号:S-AZCAS Megazyme中文站

Azo-酪蛋白(磺胺染料)

英文名:Azo-Casein (Sulphanilamide Dyed)

货号:S-AZCAS

规格:10 grams

A highly sensitive, soluble substrate for the assay of endo-protease activity. This substrate has a 5-fold greater sensitivity than similar products supplied by other companies.

蛋白内切酶活性高灵敏度检测可溶性底物[相比同类产品其灵敏度高5倍]

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柠檬酸合酶[大肠杆菌] Citrate synthase (E. coli) 货号:E-CITEC Megazyme中文站

柠檬酸合酶[大肠杆菌]

英文名:Citrate synthase (E. coli)

货号:E-CITEC

规格:2500 Units

High purity recombinant Citrate synthase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 2.3.3.1 (Modified from 4.1.3.7 in 2002).

Recombinant from E. coli. 
In 3.2 M ammonium sulphate.

Specific activity: 15.5 U/mg (25oC, pH 8.0).

Stable at 4oC for > 2 years.

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1,4-β-D-Xylotriitol 1,4-β-D-Xylotriitol 货号:O-XTRRD Megazyme中文站

1,4-β-D-Xylotriitol

英文名:1,4-β-D-Xylotriitol

货号:O-XTRRD

规格:50 mg

CAS: 116269-52-4
Molecular Formula: C15H28O13
Molecular Weight: 416.4
Purity: > 95%

High purity 1,4-β-D-Xylotriitol for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This compound can be used as a substrate for xylan degrading enzymes such as endo-1,4-β-xylanase or β-xylosidase. Borohydride reduced oligosaccharides can be used in the place of native oligosaccharides when performing enzymatic degradation experiments using reducing sugar assays. The native oligosaccharides cannot be used for this purpose because, being reducing sugars themselves, they generate a large ‘blank’ value in these assays.

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纤维素酶[1,4-D-葡聚糖内切酶[芽孢杆菌] Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens) 货号:E-CELBA Megazyme中文站

纤维素酶[1,4-D-葡聚糖内切酶[芽孢杆菌]

英文名:Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens)

货号:E-CELBA

规格:3500 Units at 40°C;

High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.4 
CAZy Family: GH5

Recombinant. From Bacillus amyloliquefaciens. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 80 U/mg (40oC, pH 6.0 on CM-cellulose 4M); ~ 160 U/mg (60oC, pH 6.0 on CM-cellulose 4M).

Stability: > 2 years at 4oC.

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2-硝基苯基β-木糖苷 2-Nitrophenyl-β-xylobioside 货号:O-ONPX2-25MG Megazyme中文站

2-硝基苯基β-木糖苷

英文名:2-Nitrophenyl-β-xylobioside

货号:O-ONPX2-25MG

规格:25 mg

CAS: 157956-98-4
Molecular Formula: C16H21NO11
Molecular Weight: 403.3
Purity: 98%

High purity 2-Nitrophenyl-β-xylobioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for assay of xylanase and xylosidase activity. 

Data booklets for each pack size are located in the Documentation tab.

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受阻β萘基β-纤维三糖 Blocked β-naphthyl-β-cellotrioside 货号:O-BNAPG3 Megazyme中文站

受阻β萘基β-纤维三糖

英文名:Blocked β-naphthyl-β-cellotrioside

货号:O-BNAPG3

规格:5 mL of 10mM substrate in DMSO (35.9 mg)

CAS: N/A
Molecular Formula: C35H42O16
Molecular Weight: 718.7
Purity: > 95%
High purity Blocked β-naphthyl-β-cellotrioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
A potential substrate for the measurement of cellulase (endo-1,4-β-glucanase) in the presence of exo-acting enzymes.
Note: Requires the use of ancialliary β-glucosidase (eg. E-BGOSPC from Megazyme) and fast blue BB salt (eg. F3378 from Sigma Aldrich).

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Azo-小麦阿拉伯木聚糖(粉末) Azo-Wheat Arabinoxylan (powder) 3g 货号:S-AWAXP Megazyme中文站

Azo-小麦阿拉伯木聚糖(粉末)

英文名:Azo-Wheat Arabinoxylan (powder) 3g

货号:S-AWAXP

规格:3 grams

Prepared by dyeing wheat flour arabinoxylan with Remazolbrilliant Blue R dye. Substrate for the assay of endo-1,4-ß-D-xylanase.

木聚糖酶检测底物

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纤维素酶[1,4-D-葡聚糖内切酶[黑曲霉] Cellulase (endo-1,4-β-D-glucanase) (Aspergillus niger) 货号:E-CELAN Megazyme中文站

纤维素酶[1,4-D-葡聚糖内切酶[黑曲霉]

英文名:Cellulase (endo-1,4-β-D-glucanase) (Aspergillus niger)

货号:E-CELAN

规格:2000 Units

High purity Cellulase (endo-1,4-β-D-glucanase) (A. niger) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.4 
CAZy Family: GH12

From Aspergillus niger. Single band on SDS PAGE. Crystalline enzyme in 3.2 M ammonium sulphate plus 0.02% sodium azide.

Specific activity: ~ 80 U/mg (40oC, pH 4.5, CM-cellulose 4M as substrate).

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1:3,1:4-β-D-低聚糖的纤维素(B) 1,3:1,4 BETA-Glucotriose (B) 50mg 货号:O-BGTRIB Megazyme中文站

1:3,1:4-β-D-低聚糖的纤维素(B)

英文名:1,3:1,4 BETA-Glucotriose (B) 50mg

货号:O-BGTRIB

规格:50 mg

CAS: 32581-36-5
Molecular Formula: C18H32O16
Molecular Weight: 504.4
Purity: > 95%

High purity 1,3:1,4-β-Glucotriose (B) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Trisaccharide produced on hydrolysis of 1:3,1:4-β-D-glucan by lichenase. Glc1-4Glc1-3Glc

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鸟苷酸激酶[原核生物] Guanylate kinase (prokaryote) 货号:E-GMPK Megazyme中文站

鸟苷酸激酶[原核生物]

英文名:Guanylate kinase (prokaryote)

货号:E-GMPK

规格:500 Units

High purity recombinant Guanylate kinase (prokaryote) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 2.7.4.8

Recombinant from a prokaryote. 
In 3.2 M ammonium sulphate.

Specific activity: 568.4 U/mg (25oC, pH 7.6).

Stable at 4oC for > 2 years.

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Azo-木聚糖(桦木) Azo-Xylan (Birchwood) 100ml 2 vials 货号:S-AXBL Megazyme中文站

Azo-木聚糖(桦木)

英文名:Azo-Xylan (Birchwood) 100ml 2 vials

货号:S-AXBL

规格:2 x 100 mL (1% w/v)

High purity dyed and crosslinked soluble Azo-Xylan (Birchwood) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

Substrate for the specific assay of endo-1,4-ß-D-xylanase.

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磷酸葡糖异构酶[酿酒酵母] Phosphoglucose isomerase (Saccharomyces cerevisiae) 货号:E-PGISC-50KU Megazyme中文站

磷酸葡糖异构酶[酿酒酵母]

英文名:Phosphoglucose isomerase (Saccharomyces cerevisiae)

货号:E-PGISC-50KU

规格:50000 units

High purity Phosphoglucose isomerase (S. cerevisiae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 5.3.1.9

From Saccharomyces cerevisiae. Electrophoretically homogeneous (MW 62,400). Single major band on isoelectric focusing (pI 6.1); minor band pI 6.9. 
In 3.2M ammonium sulphate.

Specific activity: ~ 650 U/mg of protein (25oC, pH 7.6) or ~ 850 U/mg of protein (40oC, pH 7.6).

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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