木聚糖酶M1[绿色木霉菌] endo-1,4-β-Xylanase M1 (Trichoderma viride) 货号:E-XYTR1 Megazyme中文站

木聚糖酶M1[绿色木霉菌]

英文名:endo-1,4-β-Xylanase M1 (Trichoderma viride)

货号:E-XYTR1

规格:8000 Units

High purity endo-1,4-beta-Xylanase M1 (T. viride) for use in research, biochemical enzyme assays and in vitro 
diagnostic analysis.

EC 3.2.1.8 
CAZy Family: GH:11

From T. viride. Electrophoretically homogeneous. 
In 3.2 M ammonium sulphate.

Specific activity: 230 U/mg (40oC, pH 4.5, wheat flour arabinoxylan as substrate).

Stable at 4oC for > 4 years.

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甘露聚糖酶检测片剂 (200) Mannazyme – 200 Tablets 货号:T-MNZ-200T Megazyme中文站

甘露聚糖酶检测片剂 (200)

英文名:Mannazyme – 200 Tablets

货号:T-MNZ-200T

规格:200 Tablets

High purity dyed and crosslinked Mannazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of endo-1,4-ß-D-mannanase. Containing AZCL-Galactomannan (carob).

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阿魏酸酯酶[丁酸杆菌] Feruloyl esterase (Clostridium thermocellum) 货号:E-FAEZCT Megazyme中文站

阿魏酸酯酶[丁酸杆菌]

英文名:Feruloyl esterase (Clostridium thermocellum)

货号:E-FAEZCT

规格:10 Units (~ 1800 U on FAXX)

High purity recombinant Feruloyl esterase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.1.1.73 
CAZy Family: CE1

Recombinant. Feruloyl esterase domain of xylanase XynZ (Xyn10A) from Clostridium thermocellum. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 0.6 U/mg on ethyl ferulate at 50oC and pH 6.0. This enzyme is ~ 180 fold more active on FAXX.

Stability: > 2 years at 4oC.

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4-甲基伞形酮-β-(1,3:1,4)- glucotriosid 4-Methylumbelliferyl-β-(1,3:1,4)-glucotrioside 货号:O-4MUBG3 Megazyme中文站

4-甲基伞形酮-β-(1,3:1,4)- glucotriosid

英文名:4-Methylumbelliferyl-β-(1,3:1,4)-glucotrioside

货号:O-4MUBG3

规格:10 mg

Synonyms: 4-Methylumbelliferyl β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→3)-β-D-glucopyranoside
CAS: 172364-09-9
Molecular Formula: C28H38O18
Molecular Weight: 662.6
Purity: > 90% (contains ~ 5% 4MU-α-anomer)

High purity 4-Methylumbelliferyl-β-(1,3:1,4)-glucotrioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a fluorimetric substrate for the detection or measurement of lichenase or mixed linkage β-glucanase (endo-1,3:1,4-β-D-glucanase) activity. As this substrate can also be hydrolysed by exo-acting β-glucanase/β-glucosidase enzymes, it is recommended only for the assay of pure enzyme solutions. The data sheet for the analogous UV-based substrate, 2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotrioside (cat. no. O-CNPBG3), describes general assay conditions although the substrate concentration can be significantly reduced for this fluorimetric version of the assay. Please note that a fluorimeter is required.

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甘油检测试剂盒 Glycerol GK Assay Kit 货号:K-GCROLGK Megazyme中文站

甘油检测试剂盒

英文名:Glycerol GK Assay Kit

货号:K-GCROLGK

规格:70 assays (manual) /700 assays (microplate

The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(ADP-GK)
(2) ADP + D-glucose → G-6-P + AMP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NAD+ → gluconate-6-phosphate + NADH + H+

Kit size: 70 assays (manual) / 700 (microplate)
/ 600 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 7 min
Detection limit: 0.37 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition: Novel method

Advantages

  • Novel tablet format for increased stability
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction
  • Positive reaction (assay proceeds with an increase in absorbance)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Is the K-GCROLGK Assay Kit suitable for measurement using cell culture media samples?

Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit.  It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed by dilution (if required) in distilled water. 

Q3. Is K-GCROLGK specific for glycerol?

K-GCROLGK is highly specific for glycerol.
Some compounds that are known not to react or interfere with the assay include:
Polyethylene glycol
Ethylene glycol
Propylene glycol 

Q4. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q5. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q6. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q7. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q8. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the 
K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch 
this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q9. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q10. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q11. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q12. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q13. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q14. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q15. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q16. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q17. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

Q18. To measure fermentation samples contain high microbial cell density, is cell disruption required?

Cell disruption is only require to measure glycerol within microbial cells.

To measure glycerol in the extracellular media only then cell disruption is not required and centrifugation of the sample may be sufficient, e.g.:

(a) Determination of glycerol in cell culture media/supernatants. In general, the concentration of glycerol in cell culture media/supernatants can be determined without any sample treatment (except clarification by centrifugation/filtering or dilution according to the dilution table, if necessary). Typically, no clarification or dilution is required, and a sample volume of 0.1 mL is satisfactory.

If interference is suspected then sample clarification/deproteinisation using carrez reagents or perchloric acid should be used (methods are provided in the kit booklet).

阿拉伯树胶四糖[纯度>95%][浆] Arabinotetraose (syrup) – 30mg 货号:O-ATE Megazyme中文站

阿拉伯树胶四糖[纯度>95%][浆]

英文名:Arabinotetraose (syrup) – 30mg

货号:O-ATE

规格:30 mg

CAS: 190852-24-5
Molecular Formula: C20H34O17
Molecular Weight: 546.5
Purity: > 95%



High purity Arabinotetraose (syrup) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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1,4-β-D-细胞试验(硼降低纤维三糖) 1,4-β-D-Cellotriitol (borohydride reduced cellotriose) 货号:O-CTRRD Megazyme中文站

1,4-β-D-细胞试验(硼降低纤维三糖)

英文名:1,4-β-D-Cellotriitol (borohydride reduced cellotriose)

货号:O-CTRRD

规格:50 mg

CAS: 61473-64-1
Molecular Formula: C18H34O16
Molecular Weight: 506.4
Purity: > 95%

High purity 1,4-β-D-Cellotriitol (borohydride reduced cellotriose) for use in research, biochemical enzyme assays and

in vitro diagnostic analysis.

Trisaccharides from hydrolysis of cellulose and borohydride reduced.

Store at room temperature.

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半乳糖脱氢酶/半乳糖变旋酶 Galactose dehydrogenase – Galactose mutarotase 货号:E-GALMUT Megazyme中文站

半乳糖脱氢酶/半乳糖变旋酶

英文名:Galactose dehydrogenase – Galactose mutarotase

货号:E-GALMUT

规格:1 mL; galactose dehydrogenase (200 U/mL) / galactose mutarot

High purity recombinant Galactose dehydrogenase/Galactose mutarotase for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Can be employed for the rapid determination of D-galactose or L-arabinose. See Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR) for full details.

EC 1.1.1.48 and EC 5.1.3.3, respectively.

Recombinant from E. coli. These recombinant enzymes have been expressed in E. coli and purified by affinity chromatography. Electrophoretically homogeneous. In 3.2 M ammonium sulphate.

Specific activity (of GalDH before mixing with mutarotase): 390 U/mg (25oC, pH 8.6, on D-galactose).

Stable at 4oC for > 2 years.

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谷氨酸草酰乙酸转氨酶[大肠杆菌] Glutamate oxaloacetate transaminase (E. coli) 货号:E-GOTEC Megazyme中文站

谷氨酸草酰乙酸转氨酶[大肠杆菌]

英文名:Glutamate oxaloacetate transaminase (E. coli)

货号:E-GOTEC

规格:5000

谷氨酸草酰乙酸转氨酶[大肠杆菌]

High purity Glutamate oxaloacetate transaminase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 2.6.1.1
From E. coli. In 3.2 M ammonium sulphate. Electrophoretically homogeneous.
Specific activity: ~ 40 U/mg (25°C, pH 8.5).
Stable at 4°C for > 4 years

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磷酸葡糖异构酶[枯草杆菌] Phosphoglucose isomerase (Bacillus subtilis) 货号:E-PGIBS-50KU Megazyme中文站

磷酸葡糖异构酶[枯草杆菌]

英文名:Phosphoglucose isomerase (Bacillus subtilis)

货号:E-PGIBS-50KU

规格:50000 units

High purity Phosphoglucose isomerase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 5.3.1.9

From Bacillus subtilis. Electrophoretically homogeneous (MW 51,500). Several bands on isoelectric focusing (pI 4.8-5.0).
In 3.2 M ammonium sulphate.

Specific activity: 250 U/mg of protein (25oC, pH 7.6) or 483 U/mg of protein (40oC, pH 7.6).

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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α-L-阿拉伯呋喃糖苷酶(新特异性)(青春双歧杆菌) α-L-Arabinofuranosidase (novel specificity) (Bifidobacterium adolescentis) 货号:E-AFAM2 Megazyme中文站

α-L-阿拉伯呋喃糖苷酶(新特异性)(青春双歧杆菌)

英文名:α-L-Arabinofuranosidase (novel specificity) (Bifidobacterium adolescentis)

货号:E-AFAM2

规格:400 Units

High purity alpha-L-Arabinofuranosidase (novel specificity) (B. adolescentis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.55 
CAZy Family: GH43

From Bifidobacterium adolescentis. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 90 U/mg on xylanase degraded wheat arabinoxylan (at 10 mg/mL, 40oC, pH 6.0); 
                           ~ 28 U/mg on wheat arabinoxylan (at 10 mg/mL, 40oC, pH 6.0); 
                           ~ 4 U/mg on sugar-beet arabinan; 
                           ~ 0.1 U/mg on p-nitrophenyl-α-L-arabinofuranoside.

Stable at 4oC for > 4 years.

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木聚糖酶[海栖热袍菌] endo-1,4-β-Xylanase (Thermotoga maritima) 货号:E-XYLATM Megazyme中文站

木聚糖酶[海栖热袍菌]

英文名:endo-1,4-β-Xylanase (Thermotoga maritima)

货号:E-XYLATM

规格:7500 Units at 80°C

High purity recombinant endo-1,4-beta-Xylanase (Thermotoga maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8 
CAZY Family: GH10

Recombinant. Catalytic domain of Xyn10A from Thermotoga maritima. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 115 U/mg (80oC, pH 5.0 on wheat arabinoxylan); ~ 22 U/mg (40oC, pH 5.0 on wheat arabinoxylan).

Store at 4oC.

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Beta甘露糖苷酶[纤维单胞菌] Beta-Mannosidase (C. fimi) – 200U 货号:E-BMOSCF Megazyme中文站

Beta甘露糖苷酶[纤维单胞菌]

英文名:Beta-Mannosidase (C. fimi) – 200U

货号:E-BMOSCF

规格:200 Units

High purity recombinant beta-Mannosidase (C. fimi) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.25 
CAZy Family: GH2

Recombinant from Cellulomonas fimi. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 15 U/mg (35oC; 0.8 mM p-nitrophenyl β-D-mannopyranoside; pH 6.5).

Stable at 4oC for > 2 years.

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木聚糖酶AX检测底物 Xylazyme AX – (60mg) 200 Tablets 货号:T-XAX-200T Megazyme中文站

木聚糖酶AX检测底物

英文名:Xylazyme AX – (60mg) 200 Tablets

货号:T-XAX-200T

规格:200 Tablets

High purity dyed and crosslinked Xylazyme AX (60 mg tablets) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of endo-1,4-ß-D-xylanase. Containing AZCL-arabinoxylan (wheat).

PDF Download

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葡萄糖基-麦芽三糖基-麦芽三糖 A-D-Glucosyl-Maltotriosyl-Maltriose 货号:O-GMH Megazyme中文站

葡萄糖基-麦芽三糖基-麦芽三糖

英文名:A-D-Glucosyl-Maltotriosyl-Maltriose

货号:O-GMH

规格:30 mg

CAS: 40879-32-1
Molecular Formula: C42H72O36
Molecular Weight: 1153.0
Purity: > 95%

High purity 63-alpha-D-Glucosyl-maltotriosyl-maltriose for use in research, biochemical enzyme assays and
in vitro diagnostic analysis.

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木聚糖酶[芽孢杆菌] endo-1,4-?-Xylanase (N.patriciarum) 货号:E-XYLNP Megazyme中文站

木聚糖酶[芽孢杆菌]

英文名:endo-1,4-?-Xylanase (N.patriciarum)

货号:E-XYLNP

规格:20000 Units

High purity recombinant endo-1,4-beta-Xylanase (Neocallimastix patriciarum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8
CAZy Family: GH11

Recombinant. Catalytic domain of Xyn11A from Neocallimastix patriciarum. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 1,000 U/mg (40oC, pH 6.0 on wheat arabinoxylan); 
                           ~ 1,500 U/mg (50oC, pH 6.0 on wheat arabinoxylan).

Store at 4oC.

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1,4-β-D-Xylobiitol 1,4-β-D-Xylobiitol 货号:O-XBIRD Megazyme中文站

1,4-β-D-Xylobiitol

英文名:1,4-β-D-Xylobiitol

货号:O-XBIRD

规格:50 mg

CAS: 20237-70-1
Molecular Formula: C10H20O9
Molecular Weight:
 284.3
Purity: > 95%

High purity 1,4-β-D-Xylobiitol for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This compound can be used as a substrate for xylan degrading enzymes such as endo-1,4-β-xylanase or β-xylosidase. Borohydride reduced oligosaccharides can be used in the place of native oligosaccharides when performing enzymatic degradation experiments using reducing sugar assays. The native oligosaccharides cannot be used for this purpose because, being reducing sugars themselves, they generate a large ‘blank’ value in these assays.

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普鲁兰酶M1[植生克雷伯氏菌] Pullulanase M1 (Klebsiella planticola) 货号:E-PULKP Megazyme中文站

普鲁兰酶M1[植生克雷伯氏菌]

英文名:Pullulanase M1 (Klebsiella planticola)

货号:E-PULKP

规格:700 Units

High purity Pullulanase M1 (K. planticola) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.41 
CAZy Family: GH13

From Klebsiella planticola. Electrophoretically homogeneous.
In 3.2 M ammonium sulphate.

Specific activity: 34 U/mg (40oC, pH 5.0, 1% pullulan). 

Stable at 4oC for > 4 years. 
Recommended pullulanase for research on starch structure.

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非特异性内切-1,3( 4) β葡聚糖酶(热纤梭菌) Non-specific endo-1,3(4) β-Glucanase (Clostridium thermocellum) 货号:E-LICACT Megazyme中文站

非特异性内切-1,3( 4) β葡聚糖酶(热纤梭菌)

英文名:Non-specific endo-1,3(4) β-Glucanase (Clostridium thermocellum)

货号:E-LICACT

规格:5000 Units

High purity recombinant Non-specific endo-1,3(4) beta-Glucanase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.6
CAZy Family: GH16

Recombinant: Catalytic domain of LicA from Clostridium thermocellum. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 300 U/mg (40oC, pH 6.5 on barley β-glucan); 
                           ~ 500 U/mg (60oC, pH 6.5 on barley β-glucan); 
                           ~ 7.2 U/mg (40oC, pH 6.5 on CM-curdlan).
Store at 4oC.

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磷酸葡糖异构酶[酿酒酵母] Phosphoglucose isomerase (Saccharomyces cerevisiae) 货号:E-PGISC-5KU Megazyme中文站

磷酸葡糖异构酶[酿酒酵母]

英文名:Phosphoglucose isomerase (Saccharomyces cerevisiae)

货号:E-PGISC-5KU

规格:5000 units

High purity Phosphoglucose isomerase (S. cerevisiae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 5.3.1.9

From Saccharomyces cerevisiae. Electrophoretically homogeneous (MW 62,400). Single major band on isoelectric focusing (pI 6.1); minor band pI 6.9. 
In 3.2M ammonium sulphate.

Specific activity: ~ 650 U/mg of protein (25oC, pH 7.6) or ~ 850 U/mg of protein (40oC, pH 7.6).

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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32α- L-阿拉伯呋喃糖-(1,5)-α- l-arabinotrio 32-α-L-Arabinofuranosyl-(1,5)-α-L-arabinotriose 货号:O-A4B Megazyme中文站

32α- L-阿拉伯呋喃糖-(1,5)-α- l-arabinotrio

英文名:32-α-L-Arabinofuranosyl-(1,5)-α-L-arabinotriose

货号:O-A4B

规格:20 mg

CAS: 1237488-35-5
Molecular Formula: C20H34O17
MW: 546.5
Purity: > 85%

High purity 32-α-L-arabinofuranosyl-(1,5)-α-L-arabinotriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. It can be used as an analytical standard or as a substrate to help characterise the activities of arabinoxylan degrading enzymes including endo-1,5-α-arabinanase and α-L-arabinofuranosidase. This compound was prepared by the controlled enzymatic hydrolysis of debranched sugar beet arabinan.

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磷酸甘露糖异构酶[大肠杆菌] Phosphomannose isomerase (E. coli) 货号:E-PMIEC Megazyme中文站

磷酸甘露糖异构酶[大肠杆菌]

英文名:Phosphomannose isomerase (E. coli)

货号:E-PMIEC

规格:1000 Units

High purity recombinant Phosphomannose isomerase (E. coli) for use in research, biochemical enzyme assays and 
in vitro diagnostic analysis.

EC 5.3.1.8

From E. coli. This recombinant enzyme has been expressed in E. coli and purified by affinity chromatography. Electrophoretically homogeneous (MW 43,900). 
In 3.2 M ammonium sulphate.

Specific activity: ~ 100 U/mg (25oC, pH 7.6, on mannose 6-phosphate).

Stable at 4oC for > 4 years.

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1,4-β-D-Xylotriitol 1,4-β-D-Xylotriitol 货号:O-XTRRD Megazyme中文站

1,4-β-D-Xylotriitol

英文名:1,4-β-D-Xylotriitol

货号:O-XTRRD

规格:50 mg

CAS: 116269-52-4
Molecular Formula: C15H28O13
Molecular Weight: 416.4
Purity: > 95%

High purity 1,4-β-D-Xylotriitol for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This compound can be used as a substrate for xylan degrading enzymes such as endo-1,4-β-xylanase or β-xylosidase. Borohydride reduced oligosaccharides can be used in the place of native oligosaccharides when performing enzymatic degradation experiments using reducing sugar assays. The native oligosaccharides cannot be used for this purpose because, being reducing sugars themselves, they generate a large ‘blank’ value in these assays.

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