1,4-β-D-葡糖基D-甘露糖加-1,4-β-D-甘露二糖 1,4-β-D-Glucosyl-D-Mannose plus 1,4-β-D-Mannobiose 货号:O-GMMBI Megazyme中文站

1,4-β-D-葡糖基D-甘露糖加-1,4-β-D-甘露二糖

英文名:1,4-β-D-Glucosyl-D-Mannose plus 1,4-β-D-Mannobiose

货号:O-GMMBI

规格:50 mg

CAS: 15761-61-2
14417-51-7
Molecular Formula: C12H22O11
Molecular Weight: 342.3
Purity: > 95%

High purity 1,4-β-D-Glucosyl-D-Mannose plus 1,4-β-D-Mannobiose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

Disaccharides from enzymic hydrolysis of glucomannan. 

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车前草多糖解聚酶检测片剂 Psyllazyme – 200 Tablets 货号:T-PSYL-200T Megazyme中文站

车前草多糖解聚酶检测片剂

英文名:Psyllazyme – 200 Tablets

货号:T-PSYL-200T

规格:200 Tablets

High purity dyed and crosslinked Psyllazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of enzymes causing endo-depolymerisation of psyllium polysaccharide.Containing AZCL-psyllium polysaccharide.

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木糖基纤维二糖 Xylosyl Cellobiose (reduced) 50mg 货号:O-XCBIR Megazyme中文站

木糖基纤维二糖

英文名:Xylosyl Cellobiose (reduced) 50mg

货号:O-XCBIR

规格:50 mg

CAS: 129865-02-7
Molecular Formula: C17H32O15
Molecular Weight: 476.4
Purity: > 95%

High purity Xylosyl-cellobiose (borohydride reduced) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Potential substrate for α-xylosidase.

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阿拉伯树胶三糖[纯度>95%][浆] Arabinotriose (syrup) – 50mg 货号:O-ATR Megazyme中文站

阿拉伯树胶三糖[纯度>95%][浆]

英文名:Arabinotriose (syrup) – 50mg

货号:O-ATR

规格:50 mg

CAS: 89315-59-3
Molecular Formula: C15H26O13
Molecular Weight: 414.4
Purity: > 95%



High purity Arabinotriose (syrup) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Beta葡聚糖[大麦;中黏度] Beta-Glucan (Barley;Med Viscosity) 5g 货号:P-BGBM Megazyme中文站

Beta葡聚糖[大麦;中黏度]

英文名:Beta-Glucan (Barley;Med Viscosity) 5g

货号:P-BGBM

规格:5 grams

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 1. Which barley beta-glucan do you recommend for the glucanase assay? There are three kinds of barley beta-glucan availabale from Megazyme – high viscosity, medium viscosity and low viscosity.

For glucanase assay, we recommend the medium viscosity beta-glucan.  We now offer low, medium and high viscosity wheat arabinoxylans, and we think that the low viscosity material will be best (easiest) to use in the reducing-sugar assay.

2. Both substrates; barley beta-glucan and wheat arabinoxylan, are standardised to a specific viscosity, e.g. 23-24 cSt. Are the substrates adjusted to give this viscosity?

The substrates are enzymically treated to yield the required viscosity (20 ~ 30 cSt). Modification of the viscosity of beta-glucan is required for IOB viscometric malt beta-glucanase test (which works well).

3. I would like to vacuum dry the standards instead of measuring the moisture content. I wish to dry them at a temperature lower than used in the preparation in Megazyme. Would you please give me some suggestions?

We suggest that you dry the β-glucan powders further in a vacuum oven at about 50˚C.  This will lower the moisture content to approximately 1-2%.

4. I understand a specific polymer can be used for the assay of malt β-glucanase and celluloses. I would very much like to know which polymer this β-glucan is?

The polymer you require is barley beta-glucan (medium viscosity).  The beta-glucan that we supply is pure 1,3:1,4-beta-D-glucan.  Barley β-glucan is also susceptible to hydrolysis by cellulase.

5. Although not specified on the beta-glucan data sheet, is the ratio of 1-3 to 1-4 bonds measured? If so, what is the ratio and would you expect it to remain standardised over a number of different batches?

The content of 1,3 bonds in barley beta-glucan is about 32% (from literature).  We would not expect this to change much (if at all) over different batches.

6. We have measured the molecular weight of beta-glucan originating from barley ordered from your company. Can you please tell us which method have you used for measuring molecular weight?

The MW’s were determined by Multiangle laser light scattering technique.

7. I have purchased barley beta-glucan (lot 30108) and carob galactomannan low viscosity (lot 30702) and would like to know what else there might be in these substrates.

Barley beta-glucan lot 30108 would contain about 3-4% arabinoxylan (this was produced in 1993).  Material supplied post 1995 contains < 0.5% arabinoxylan.  Carob galactomannan is quite pure (> 96%).

8. Low, Medium, High Viscosity β-Glucans, are they naturally occurring at these MW’s or are the MW fractions obtained by some other process?

The high molecular weight beta-glucan is the naturally occurring; this is enzymically modified to give the other viscosities. 

1,2-α-L-岩藻糖苷酶(微生物) 1,2-a-L-Fucosidase (microbial) 货号:E-FUCM Megazyme中文站

1,2-α-L-岩藻糖苷酶(微生物)

英文名:1,2-a-L-Fucosidase (microbial)

货号:E-FUCM

规格:3 Units

High purity 1,2-alpha-L-Fucosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

EC 3.2.1.63
CAZy Family: GH95

2-alpha-L-fucopyranosyl-beta-D-galactoside fucohydrolase 

Recombinant from microbial source. In solution (Tris.HCl/NaCl/EDTA).
Hydrolysis of terminal non-reducing α-1,2-linked L-fucose residues from glycoproteins and oligosaccharides.

Specific activity: ~ 160 U/mg (37oC, pH 6.5 on 2’-fucosyllactose)

Stability:  > 3 years at 4oC.

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Alpha淀粉酶[苔藓杆菌] Alpha-Amylase (B. licheniformis) 货号:E-BLAAM-10ML Megazyme中文站

Alpha淀粉酶[苔藓杆菌]

英文名:Alpha-Amylase (B. licheniformis)

货号:E-BLAAM-10ML

规格:10mL – 3000 units/mL

High purity alpha-Amylase (B. licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.1
CAZy Family: GH13

Alpha-Amylase. From Bacillus licheniformis. Highly purified. For use in Megazyme Total Starch and Dietary Fiber methods. Stabilised solution.

Specific activity: 55 U/mg (40oC, pH 6.0, Ceralpha reagent as substrate).

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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五糖(X3Glc4 – 硼氢化还原) Heptasaccharide Reduced 货号:O-X3G4R Megazyme中文站

五糖(X3Glc4 – 硼氢化还原)

英文名:Heptasaccharide Reduced

货号:O-X3G4R

规格:50 mg

CAS: 129865-03-8
Molecular Formula: C39H68O33
Molecular Weight: 1064.9
Purity: 95%

High purity Heptasaccharide (X3Glc4 – borohydride reduced) for use in research, biochemical enzyme assays and
in vitro diagnostic analysis.

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淀粉葡萄糖苷酶[黑曲霉] Amyloglucosidase (A. niger) 货号:E-AMGDF-40ML Megazyme中文站

淀粉葡萄糖苷酶[黑曲霉]

英文名:Amyloglucosidase (A. niger)

货号:E-AMGDF-40ML

规格:140000

High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3 
CAZy Family: GH15

Amyloglucosidase.

From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Electrophoretically homogeneous. Stabilised liquid in 50% glycerol. 

Specific activity: approx. 36 U/mg (40oC, pH 4.5, soluble starch as substrate). 200 U/ml on p-nitrophenyl β-maltoside; 3,260 U/ml on soluble starch.

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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4-甲基伞形-β纤维二糖苷 4-Methylumbelliferyl-β-cellobioside 货号:O-4MUG2 Megazyme中文站

4-甲基伞形-β纤维二糖苷

英文名:4-Methylumbelliferyl-β-cellobioside

货号:O-4MUG2

规格:200 mg

CAS: 72626-61-0
Molecular Formula: C22H28O13
Molecular Weight: 500.4
Purity: > 95%

A substrate for research into cellulase (endo-1,4-β-glucanase) or cellulose degrading enzymes.

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潘糖 Panose 货号:O-PAN-1G Megazyme中文站

潘糖

英文名:Panose

货号:O-PAN-1G

规格:1 g

CAS: 33401-87-5
Molecular Formula: C18H32O16
Molecular Weight: 504.4
Purity: > 97%

High purity Panose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Panose is formed by the action of neopullulanase on pullulan. It can be used as an analytical standard or as a substrate to help characterise the activities of other starch degrading enzymes including α-glucosidase and amyloglucosidase.

Data booklets for each pack size are located in the Documentation tab.

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木聚糖酶检测试剂盒 Xylanase Assay Kit (Xylazyme AX) 货号:K-XYLS Megazyme中文站

木聚糖酶检测试剂盒

英文名:Xylanase Assay Kit (Xylazyme AX)

货号:K-XYLS

规格:200 assays per kit

The Xylanase (Xylazyme AX) test kit is used for the measurement and analysis of endo-1,4-β-D-xylanase in enzyme preparations, bread improver mixtures and animal feeds. Contains Xylazyme AX Tablets and xylanase enzyme controls (A. niger and Trichoderma longibrachiatum).

Colourimetric method for the determination of Xylanase in feed,
foodstuffs and other materials

Principle:
(β-xylanase)
(1) Xylazyme AX (water insoluble) + H2O → water soluble dyed xylan fragments

Kit size: 200 assays
Method: Based on use of Xylazyme AX tablets (590 nm)
Total assay time: ~ 45 min
Detection limit: 0.02 U/mL of assay solution
Application examples:
Animal feeds, enzyme preparations, bread improver mixtures and
other materials
Method recognition:
Used widely in the feed industry

Advantages

  • Very cost effective
  • All reagents stable for > 2 years during use
  • Only test kit available
  • Simple format
  • Standard included

 

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Beta淀粉酶[大麦] Beta-Amylase (Barley; powder) 货号:E-BARBP-5G Megazyme中文站

Beta淀粉酶[大麦]

英文名:Beta-Amylase (Barley; powder)

货号:E-BARBP-5G

规格:5 grams

High purity β-Amylase (Barley) (powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.2 
CAZy Family: GH14

From barley flour. Crystalline. 
In BSA plus ammonium sulphate powder.

Stable at -20oC for > 4 years. 
Powder enzyme form (2000°L) is for use in AACC and ASBC α-amylase assay procedures.

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三乙酰壳三糖 Triacetyl-Chitotriose 30mg 货号:O-CHI3 Megazyme中文站

三乙酰壳三糖

英文名:Triacetyl-Chitotriose 30mg

货号:O-CHI3

规格:30 mg

CAS: 38864-21-0
Molecular Formula: C24H41N3O16
Molecular Weight: 627.6
Purity: 95%

High purity Triacetyl-chitotriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Prepared from chitin.

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Alpha葡萄糖醛酸酶[分离嗜热菌] α-Glucuronidase (Geobacillus stearothermophilus) (Recombinant) 货号:E-AGUBS Megazyme中文站

Alpha葡萄糖醛酸酶[分离嗜热菌]

英文名:α-Glucuronidase (Geobacillus stearothermophilus) (Recombinant)

货号:E-AGUBS

规格:200 Units at 70°C

High purity recombinant alpha-D-Glucuronidase (Geobacillus stearothermophilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.139
CAZy Family: GH67

Recombinant from Geobacillus stearothermophilus. 
In 3.2 M ammonium sulphate.
Hydrolysis of the α-1,2 glycosidic bond between D-glucuronic acid or it’s ether 4-O-methyl-D-glucuronic acid and 
D-xylose residues of xylo-oligosaccharides (aldouronic acids) from xylan.

Specific activity: ~ 7 U/mg (70oC, pH 7.0 on aldouronic acid).

Stability > 2 years 4oC.

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L-苹果酸脱氢酶[大肠杆菌] L-Malate dehydrogenase (E. coli) 货号:E-LMDHEC Megazyme中文站

L-苹果酸脱氢酶[大肠杆菌]

英文名:L-Malate dehydrogenase (E. coli)

货号:E-LMDHEC

规格:50000 Units

High purity recombinant L-Malate dehydrogenase (E. coli) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 1.1.1.37

From E. coli. This recombinant enzyme has been expressed in E. coli and purified by affinity chromatography. Electrophoretically homogeneous (MW 34,500). 
In 3.2 M ammonium sulphate. 

Specific activity: 1130 U/mg (25oC, pH 7.5. on oxaloacetic acid.

Stable at 4oC for > 4 years.

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1,4-β-D-葡糖基-D-甘露二糖加1,4-β-D-纤维二糖苷-D-甘露糖 1,4-β-D-Glucosyl-D-Mannobiose plus 1,4-β-D-Cellobiosyl-D-Mannose 货号:O-GMMTR Megazyme中文站

1,4-β-D-葡糖基-D-甘露二糖加1,4-β-D-纤维二糖苷-D-甘露糖

英文名:1,4-β-D-Glucosyl-D-Mannobiose plus 1,4-β-D-Cellobiosyl-D-Mannose

货号:O-GMMTR

规格:50 mg

CAS: 28072-83-5
          28072-82-4
Molecular Formula: C18H32O16
Molecular Weight: 504.4
Purity: > 90%

High purity 1,4-β-D-Glucosyl-D-Mannobiose and 1,4-β-D-Cellobiosyl-D-Mannose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

Trisaccharides from hyrolysis of glucomannan. 

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1,4-b-D-葡聚糖纤维二糖水解酶[单胞菌] β-D-Xylosidase (Selenomonas ruminantium) 货号:E-BXSR-3KU Megazyme中文站

1,4-b-D-葡聚糖纤维二糖水解酶[单胞菌]

英文名:β-D-Xylosidase (Selenomonas ruminantium)

货号:E-BXSR-3KU

规格:3000 units

High purity recombinant exo-1,4-beta-D-Xylosidase (S. ruminantium) for use in research, biochemical enzyme assays andin vitro diagnostic analysis.

EC 3.2.1.37 
CAZy Family: GH43

1,4-β-D-xylan xylohydrolase.

Recombinant from S. ruminantium. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 115 U/mg (40oC, pH 5.3 on pNP-β-D-xylanopyranoside).

Data booklets for each pack size are located in the Technical Resources tab.

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