32α- L-阿拉伯呋喃糖-(1,5)-α- l-arabinotrio 32-α-L-Arabinofuranosyl-(1,5)-α-L-arabinotriose 货号:O-A4B Megazyme中文站

32α- L-阿拉伯呋喃糖-(1,5)-α- l-arabinotrio

英文名:32-α-L-Arabinofuranosyl-(1,5)-α-L-arabinotriose

货号:O-A4B

规格:20 mg

CAS: 1237488-35-5
Molecular Formula: C20H34O17
MW: 546.5
Purity: > 85%

High purity 32-α-L-arabinofuranosyl-(1,5)-α-L-arabinotriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. It can be used as an analytical standard or as a substrate to help characterise the activities of arabinoxylan degrading enzymes including endo-1,5-α-arabinanase and α-L-arabinofuranosidase. This compound was prepared by the controlled enzymatic hydrolysis of debranched sugar beet arabinan.

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普鲁兰酶M2[对地衣芽孢杆菌] Pullulanase M2 (Bacillus licheniformis) 货号:E-PULBL Megazyme中文站

普鲁兰酶M2[对地衣芽孢杆菌]

英文名:Pullulanase M2 (Bacillus licheniformis)

货号:E-PULBL

规格:2000 Units

High purity Pullulanase M2 (B. licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.41

From Bacillus licheniformis. Electrophoretically homogeneous (MW 113,000). 
In 3.2M ammonium sulphate.

Specific activity: ~ 40 U/mg (40oC, pH 5.0, pullulan as substrate).

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Acetyl-CoA合成酶[枯草杆菌] RecAcetyl-CoA Synthetase (B. subtilis) 货号:E-ACSBS Megazyme中文站

Acetyl-CoA合成酶[枯草杆菌]

英文名:RecAcetyl-CoA Synthetase (B. subtilis)

货号:E-ACSBS

规格:250 Units

High purity Acetyl-CoA synthetase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 6.2.1.1

From B. subtilis. 
In 3.2 M ammonium sulphate.

Specific activity: 7.6 U/mg (37oC, pH 7.5); 39 U/mg (37oC, pH 8.4); 7.0 U/mg (25oC, pH 7.5).

Stable at 4oC for > 2 years.

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磷酸葡糖异构酶[酿酒酵母] Phosphoglucose isomerase (Saccharomyces cerevisiae) 货号:E-PGISC-5KU Megazyme中文站

磷酸葡糖异构酶[酿酒酵母]

英文名:Phosphoglucose isomerase (Saccharomyces cerevisiae)

货号:E-PGISC-5KU

规格:5000 units

High purity Phosphoglucose isomerase (S. cerevisiae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 5.3.1.9

From Saccharomyces cerevisiae. Electrophoretically homogeneous (MW 62,400). Single major band on isoelectric focusing (pI 6.1); minor band pI 6.9. 
In 3.2M ammonium sulphate.

Specific activity: ~ 650 U/mg of protein (25oC, pH 7.6) or ~ 850 U/mg of protein (40oC, pH 7.6).

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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麦芽Beta葡聚糖酶和纤维素内切酶检测片剂 (1000) Beta-Glucazyme – 1000 Tablets 货号:T-BGZ-1000T Megazyme中文站

麦芽Beta葡聚糖酶和纤维素内切酶检测片剂 (1000)

英文名:Beta-Glucazyme – 1000 Tablets

货号:T-BGZ-1000T

规格:1000 Tablets

High purity dyed and crosslinked beta-Glucazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

RACI Standard Method. For the assay of malt ß-glucanase and endo-cellulase.Containing AZCL-Barley ß-glucan.

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L-海藻糖检测试剂盒 L-Fucose Assay Kit 货号:K-FUCOSE Megazyme中文站

L-海藻糖检测试剂盒

英文名:L-Fucose Assay Kit

货号:K-FUCOSE

规格:100 assays (manual) /

The L-Fucose test kit is a simple, rapid and reliable method, for the measurement and analysis of L-Fucose in plant extracts, biological samples and other materials. This kit can be used in the measurement of α-fucosidases that do not act on chromogenic substrates.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of L-Fucose in plant material,
polysaccharides, pharmaceuticals and other materials

Principle:
(L-fucose dehydrogenase)
(1) L-Fucose + NADP+ → L-fucono-1,5-lactone + NADPH + H+

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1020 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 10 min
Detection limit: 15.4 mg/L
Application examples:
L-Fucose is present as the main component in fucoidan (a marine
polysaccharide), foods, pharmaceuticals and other materials
(e.g. biological samples, etc.)
Method recognition: Novel method

Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Simple format
  • Rapid reaction time (~ 10 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. Can oligosaccharides or polysaccharides be measured using the kit assay?

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

Q5. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q6. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q7. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q8. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q9. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q10. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q11. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q12. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q14. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

Beta淀粉酶[大麦] Beta-Amylase (Barley; liquid) 50KU 货号:E-BARBL-50KU Megazyme中文站

Beta淀粉酶[大麦]

英文名:Beta-Amylase (Barley; liquid) 50KU

货号:E-BARBL-50KU

规格:50000 units

High purity beta-Amylase (Barley) liquid enzyme for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.2
CAZy Family: GH14
From barley flour. Crystalline.
In 3.2 M ammonium sulphate.
Specific activity: ca. 620 U/mg (40oC, pH 6.0, soluble starch as substrate).
Stable at 4oC for > 4 years.
Powder enzyme form (2000°L) is for use in AACC and ASBC α-amylase assay procedures (see E-BARBP).
Data booklets for each pack size are located in the Technical Resources tab.

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Alpha淀粉酶高灵敏度检测底物 Amylazyme Red – 1000 Tablets 货号:T-AMZRD-1000T Megazyme中文站

Alpha淀粉酶高灵敏度检测底物

英文名:Amylazyme Red – 1000 Tablets

货号:T-AMZRD-1000T

规格:1000 Tablets

High purity dyed and crosslinked Amylazyme Red tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

A highly sensitive substrate for the measurement of α-amylase.

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极限糊精酶和支链淀粉酶检测片剂 (200) Limit-Dextrizyme – 200 Tablets 货号:T-LDZ-200T Megazyme中文站

极限糊精酶和支链淀粉酶检测片剂 (200)

英文名:Limit-Dextrizyme – 200 Tablets

货号:T-LDZ-200T

规格:200 Tablets

High purity dyed and crosslinked Limit-Dextrizyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

RACI Standard Method. For the assay of limit-dextrinase and pullulanase. Containing AZCL-Pullulan.

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2-氯-4-硝基苯基β-(1,3:1,4)-glucopyranoside 2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotetraoside 货号:O-CNPBG4 Megazyme中文站

2-氯-4-硝基苯基β-(1,3:1,4)-glucopyranoside

英文名:2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotetraoside

货号:O-CNPBG4

规格:20 mg

Synonyms: 2-Chloro-4-nitrophenyl β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→3)-β-D-glucopyranoside
CAS: N/A
Molecular Formula: C30H44ClNO23
Molecular Weight: 822.1
Purity: > 97%

High purity 2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotetraoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement of lichenase or mixed linkage
β-glucanase (endo-1,3:1,4-β-D-glucanase) activity. As this substrate can also be hydrolysed by exo-acting β-glucanase/β-glucosidase enzymes, it is recommended only for the assay of pure lichenase solutions. The data sheet for the analogous trisaccharide substrate, 2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotrioside (cat. no. O-CNPBG3), describes suitable assay conditions.

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地衣聚糖酶[1,3(4)-D-葡聚糖内切酶[芽孢杆菌] Lichenase (endo-1,3(4)-β-D-Glucanase) (Bacillus sp.) 货号:E-LICHN Megazyme中文站

地衣聚糖酶[1,3(4)-D-葡聚糖内切酶[芽孢杆菌]

英文名:Lichenase (endo-1,3(4)-β-D-Glucanase) (Bacillus sp.)

货号:E-LICHN

规格:5000 Units

High purity Lichenase (endo-1,3(4)-beta-Glucanase) (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.73 
CAZy Family: GH16

From B. subtilis. Electrophoretically homogeneous. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 240 U/mg (40oC, pH 6.5, barley β-glucan). 1000 U/ml. 

Stable at 4oC for > 4 years.

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葡萄糖氧化酶/催化酶混合物 Glucose oxidase – Catalase Mixture (eukaryote) 货号:E-GOXCA Megazyme中文站

葡萄糖氧化酶/催化酶混合物

英文名:Glucose oxidase – Catalase Mixture (eukaryote)

货号:E-GOXCA

规格:2 vials – 200 tests

High purity Glucose oxidase/Catalase Mixture (eukaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in removal of excess glucose in conjunction with the Sucrose/Glucose Assay Kit (K-SUCGL) and the Fructan HK Assay Kit (K-FRUCHK). Sufficient reagent for 200 incubations.

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纤维素内切酶检测底物 (200) Cellazyme C – 200 Tablets 货号:T-CCZ-200T Megazyme中文站

纤维素内切酶检测底物 (200)

英文名:Cellazyme C – 200 Tablets

货号:T-CCZ-200T

规格:200 Tablets

High purity dyed and crosslinked Cellazyme C tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of endo-cellulase. Containing AZCL-HE-Cellulose. Recommended substrate for the assay of endo-cellulase.

 

 

 

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内切-1,3 – β-D-葡聚糖酶(大麦)(重组) endo-1,3-β-D-Glucanase (barley) (Recombinant) 货号:E-LAMHV Megazyme中文站

内切-1,3 – β-D-葡聚糖酶(大麦)(重组)

英文名:endo-1,3-β-D-Glucanase (barley) (Recombinant)

货号:E-LAMHV

规格:5000 Units

High purity recombinant endo-1,3-beta-Glucanase (Barley) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.39
CAZy Family: GH17

Recombinant. From barley (Hordeum vulgare). 
In 50% glycerol.

Specific activity: ~ 100 U/mg (40oC, pH 5.0 on laminarin)

Store at -20oC. 

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果胶酸裂合酶M2[日本龟蜡蚧] Pectate lyase (Cellvibrio japonicus) 货号:E-PLYCJ Megazyme中文站

果胶酸裂合酶M2[日本龟蜡蚧]

英文名:Pectate lyase (Cellvibrio japonicus)

货号:E-PLYCJ

规格:2500 Units

High purity recombinant Pectate lyase (C. japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 4.2.2.2 
CAZy Family: PL10

Recombinant. Catalytic domain of Pel10A from Cellvibrio japonicus. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 600 U/mg protein on polygalacturonic acid (pH 10.0, 40oC).

Stable at 4oC for > 4 years.

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α – L-阿拉伯呋喃糖苷(玉蜀黍黑粉菌) α-L-Arabinofuranosidase (Ustilago maydis) 货号:E-ABFUM Megazyme中文站

α – L-阿拉伯呋喃糖苷(玉蜀黍黑粉菌)

英文名:α-L-Arabinofuranosidase (Ustilago maydis)

货号:E-ABFUM

规格:400 U at 40°C

High purity α-L-Arabinofuranosidase (Ustilago maydis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.55
CAZY Family: GH62
CAS: 9067-74-7

non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase

Recombinant. From Ustilago maydis.

In 3.2 M ammonium sulphate.

Specific activity: ~ 25 U/mg protein (40°C, pH 5.0 on wheat arabinoxylan)

Stability: > 2 years 4°C

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葡萄糖-6-磷酸脱氢酶(大肠杆菌) Glucose 6-phosphate dehydrogenase (E. coli) 货号:E-GPDHEC Megazyme中文站

葡萄糖-6-磷酸脱氢酶(大肠杆菌)

英文名:Glucose 6-phosphate dehydrogenase (E. coli)

货号:E-GPDHEC

规格:5000 Units

High purity recombinant Glucose 6-phosphate dehydrogenase (E. Coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 1.1.1.49

Recombinant from E. coli. 
In 3.2 M ammonium sulphate.
Supplied at ~ 1,250 U/mL. 

Specific activity: 172 U/mg (25oC, pH 7.6, D-glucose-6-phosphate).

Stable at 4oC for > 2 years.

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