木葡聚糖酶(GH74)(类芽孢sp。) Xyloglucanase (GH74) (Paenibacillus sp.) 货号:E-XGP74 Megazyme中文站

木葡聚糖酶(GH74)(类芽孢sp。)

英文名:Xyloglucanase (GH74) (Paenibacillus sp.)

货号:E-XGP74

规格:200 Units at 70°C

High purity recombinant exo-alpha-Sialidase (S. Typhimurium) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

EC 3.2.1.18
CAZy Family: GH33

Recombinant. From Salmonella typhimurium. In solution (Tris.HCl / NaCl / EDTA). Hydrolysis of unbranched, non-reducing terminal α-2,3-linked >> α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates.
Supplied at ~ 2,500 U/mL. 

Specific activity: ~ 750 U/mg (37oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid)

Store at 4oC.  

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1,4-b-D-葡聚糖纤维二糖水解酶[单胞菌] β-D-Xylosidase (Selenomonas ruminantium) 货号:E-BXSR-1KU Megazyme中文站

1,4-b-D-葡聚糖纤维二糖水解酶[单胞菌]

英文名:β-D-Xylosidase (Selenomonas ruminantium)

货号:E-BXSR-1KU

规格:1000 units

EC 3.2.1.37
CAZY Family: GH43

1,4-β-D-xylan xylohydrolase. Recombinant from S. ruminantium. In 3.2 M ammonium sulphate.
Specific activity: ~ 115 U/mg (40oC, pH 5.3 on p-NP-β-D-xylanopyranoside).
Other activities: p-NP-α-L-arabinofuranoside ~ 7 %.

 

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一级氨基氮[NOPA]检测试剂盒 Primary Amino Nitrogen Assay Kit (PANOPA) Assay Kit 货号:K-PANOPA Megazyme中文站

一级氨基氮[NOPA]检测试剂盒

英文名:Primary Amino Nitrogen Assay Kit (PANOPA) Assay Kit

货号:K-PANOPA

规格:100 assays (manual) / 1000 assays (microplate)

The Primary Amino Nitrogen (PANOPA) Assay Kit is suitable for the measurement and analysis of primary amino nitrogen in grape juice/must and wine.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Primary Amino Nitrogen in
grape juice, must, wine and other materials

Principle:
(room temperature)
(1) Amino nitrogen + N-acetyl-L-cysteine + o-phthaldialdehyde →
isoindole derivative

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1100 (autoanalyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 2.59 mg N/L
Application examples:
Grape juice, must, wine and other materials
Method recognition: Novel method

Advantages

  • Simple format (absorbances read at 340 nm)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

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L-乳酸检测试剂盒 L-Lactic Acid (L-Lactate) Assay Kit 货号:K-LATE Megazyme中文站

L-乳酸检测试剂盒

英文名:L-Lactic Acid (L-Lactate) Assay Kit

货号:K-LATE

规格:50 assays (manual) / 500 assays

分析物意义:水果、蔬菜和蛋产品的质量指标  

Megazyme检测试剂盒优点:反应快、试剂稳定。适用于手工和自动分析仪进行检测 

The L-Lactic Acid (L-Lactate) Assay Kit is used for the specific measurement and analysis of L-lactic acid (L-lactate) in beverages, meat, dairy and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of L-Lactic Acid in foodstuffs,
beverages and other materials

Principle:
(L-lactate dehydrogenase)
(1) L-Lactic acid + NAD+ ↔ pyruvate + NADH + H+

(glutamate-pyruvate transaminase)
(2) Pyruvate + D-glutamate → D-alanine + 2-oxoglutarate

Kit size: 50 assays (manual) / 450 (microplate)
/ 500 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 10 min
Detection limit: 0.21 mg/L
Application examples:
Wine, beer, soft drinks, milk, dairy products (e.g. cream, milk / whey
powder, cheese, condensed milk and yogurt), foods containing milk
(e.g. dietetic foods, bakery products, baby food, chocolate, sweets
and ice-cream), egg, egg products (e.g. egg powder), baking additives,
vinegar, fruit and vegetables, processed fruit and vegetables
(e.g. tomatoes), meat products, food additives, feed, paper (and
cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by DIN, GOST,
IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAK

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

Q1. Is the L-Lactic Acid (L-Lactate) Assay Kit (K-LATE) suitable for measurement using cell culture media samples?

Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit.  It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed, by dilution (if required) in distilled water. 

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. Can perchloric acid be used to deproteinise / clarify samples prior to analysis using the L-Lactic Acid Assay Kit (K-LATE)? If so, how should such an extraction be performed?

Yes.  Perchloric acid extraction can be used in conjunction with this kit, and should be performed as follows:
WARNING: If you have not worked with perchloric acid before, you must consult your safety officer for advice.  Also, depending on the nature of the samples, it may be possible to reduce the concentration of perchloric acid, to for example 0.3 M (i.e. in the case of plasma).  It is thus very important to determine if this is possible for each type of sample used, in order to reduce the risk from working with concentrated perchloric acid.

Liquid samples:

  1. Carefully add an equal volume of ice cold 3 M perchloric acid and homogenise / fully disperse the sample (as appropriate).
  2. After 15 min incubation on ice (or in a refrigerator), centrifuge at 3000 x g for 15 min at 4˚C.
  3. Neutralise by the slow addition of 2 M KOH.
  4. Incubate on ice (or in a refrigerator) until the potassium perchlorate has settled out by gravity (approximately 10 min), and then simply remove some of the clear supernatant and use directly in the assay.

 

Solid samples:

  1. Accurately weigh approx. 5 g of homogenised sample into a beaker containing 20 mL of 1 M perchloric acid and very carefully homogenise with an Ultraturrax®  (or equivalent) for 5 min.
  2. Carefully add approx. 40 mL of distilled water and neutralise using 2 M KOH (using pH test strips for example).  Quantitatively transfer the contents to a 100 mL volumetric flask and fill to the mark with distilled water.  If a fat layer develops, make sure this is above the mark, and the aqueous layer is at the mark.
  3. Incubate in a refrigerator for approx. 20 min to allow separation of fat and precipitation of potassium perchlorate.
  4. Filter through Whatman No. 1 filter paper, discarding the first few mL of filtrate, and use directly in the assay.

Q6. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q7. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q8. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the 
K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch 
this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q9. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q10. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q11. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q12. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q1

4-硝基苯基-α- l-fucopyranosid 4-Nitrophenyl-α-L-fucopyranoside 货号:O-PNPAFC Megazyme中文站

4-硝基苯基-α- l-fucopyranosid

英文名:4-Nitrophenyl-α-L-fucopyranoside

货号:O-PNPAFC

规格:500 mg

Synonyms: p-Nitrophenyl-α-L-fucopyranoside, 
                   pNP-α-L-fucopyranoside
CAS: 10231-84-2
Molecular Formula: C11H13NO
7
Molecular Weight: 271.2
Purity: > 98%

High purity 4-Nitrophenyl-α-L-fucopyranoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement of α-L-fucopyranosidase activity.

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木聚糖酶[嗜热脂肪芽孢杆菌] endo-1,4-β-Xylanase (Bacillus stearothermophilus T6) 货号:E-XYNBS Megazyme中文站

木聚糖酶[嗜热脂肪芽孢杆菌]

英文名:endo-1,4-β-Xylanase (Bacillus stearothermophilus T6)

货号:E-XYNBS

规格:2000 Units at 70°C

High purity recombinant endo-1,4-beta-Xylanase (Bacillus stearothermophilus T6) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8 
CAZy Family: GH10

Recombinant. From Bacillus stearothermophilus T6. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 65 U/mg (70oC, pH 6.5 on wheat arabinoxylan); ~ 12 U/mg (40oC, pH 6.5 on wheat arabinoxylan).

Store at 4oC.

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二乙酰壳二糖 Diacetyl-Chiotobiose – 30mg 货号:O-CHI2 Megazyme中文站

二乙酰壳二糖

英文名:Diacetyl-Chiotobiose – 30mg

货号:O-CHI2

规格:30 mg

CAS: 35061-50-8
Molecular Formula: C16H28N2O11
Molecular Weight: 424.4
Purity: > 95%

High purity Diacetyl-chitobiose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Prepared from chitin.

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果聚糖[HK法]检测试剂盒 Fructan HK Assay Kit 货号:K-FRUCHK Megazyme中文站

果聚糖[HK法]检测试剂盒

英文名:Fructan HK Assay Kit

货号:K-FRUCHK

规格:50 assays per kit

分析物意义:许多食品如洋葱和种子中的常见组分

Megazyme检测试剂盒优点:方法新颖、反应快、试剂稳定 

The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharide.

UV-method for the determination of Fructan in foodstuffs,
beverages and other materials

Principle:
(sucrase + maltase)
(1) Sucrose + maltosaccharides + H2O → D-glucose + D-fructose

(exo-inulinase + endo-inulinase)
(2) Fructan + H2O → D-glucose + D-fructose

(hexokinase)
(3) D-Glucose + D-fructose + ATP → G-6-P + F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphoglucose isomerase)
(5) F-6-P ↔ G-6-P

Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Flours, plant materials (e.g. onion), food products and other materials
Method recognition:
This method is a modification of AOAC Method 999.03 and AACC
Method 32-32.01

Advantages

  • Very cost effective
  • All reagents stable for > 12 months after preparation
  • Fructan kits are available only from Megazyme
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q5. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q6. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q7. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q8. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q9. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q10. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q11. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q12. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

Alpha淀粉酶[猪胰腺] α-Amylase (Porcine Pancreatic) 货号:E-PANAA-9G Megazyme中文站

Alpha淀粉酶[猪胰腺]

英文名: α-Amylase (Porcine Pancreatic)

货号:E-PANAA-9G

规格:9g

High purity alpha-Amylase (Porcine Pancreatic) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.1 
CAZy Family: GH13

From porcine pancreas. Partially purified. Free flowing powder.

Specific activity: ca. 100,000 U/g (40oC, Ceralpha reagent).

Stable at -20oC for > 4 years.

For use in total dietary fiber (including resistant starch) assay procedure.

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受阻4-硝基苯基α-麦芽七 Blocked 4-nitrophenyl-α-maltoheptaoside 货号:O-BPNPC7 Megazyme中文站

受阻4-硝基苯基α-麦芽七

英文名:Blocked 4-nitrophenyl-α-maltoheptaoside

货号:O-BPNPC7

规格:4 vials

CAS: 109055-07-4
Molecular Formula: C55H79NO38
Molecular Weight:1362.2
Purity:  98%

High purity Blocked 4-nitrophenyl-α-maltoheptaoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

One vial contains the same quantity of Blocked 4-nitrophenyl-α-D-maltoheptaoside as in one vial of "cereal α-amylase assay reagent" (i.e. 54.5 mg/vial). This can be used in a two-step format to assay α-amylases at pH and temperature values outside the range in which the standard reagent can be used. This preparation does not contain added enzymes.

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木糖基纤维二糖 Xylosyl Cellobiose (reduced) 50mg 货号:O-XCBIR Megazyme中文站

木糖基纤维二糖

英文名:Xylosyl Cellobiose (reduced) 50mg

货号:O-XCBIR

规格:50 mg

CAS: 129865-02-7
Molecular Formula: C17H32O15
Molecular Weight: 476.4
Purity: > 95%

High purity Xylosyl-cellobiose (borohydride reduced) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Potential substrate for α-xylosidase.

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2-氯-4-硝基苯基β-纤维二糖苷 2-Chloro-4-nitrophenyl-β-cellobioside 货号:O-CPNPG2-100 Megazyme中文站

2-氯-4-硝基苯基β-纤维二糖苷

英文名:2-Chloro-4-nitrophenyl-β-cellobioside

货号:O-CPNPG2-100

规格:100 mg

CAS: 135743-28-1
Molecular Formula: C18H24CINO13
Molecular Weight: 497.8
Purity: > 98%

High purity 2-Chloro-4-nitrophenyl-β-cellobioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

A potential substrate for the measurement of cellulase (endo-1,4-β-glucanase).

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纤维素酶[1,4-D-葡聚糖内切酶[海栖热袍菌] Cellulase (endo-1,4-β-D-glucanase) (Thermotoga maritima) 货号:E-CELTM Megazyme中文站

纤维素酶[1,4-D-葡聚糖内切酶[海栖热袍菌]

英文名:Cellulase (endo-1,4-β-D-glucanase) (Thermotoga maritima)

货号:E-CELTM

规格:2000 Units at 80°C

High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Thermotoga maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.4 
CAZy Family: GH5

Recombinant: From Thermotoga maritima. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 35 U/mg (40oC, pH 6.0 on CM-cellulose 4M); ~ 160 U/mg (80oC, pH 6.0 on CM-cellulose 4M).

Stability: > 2 years at 4oC.

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淀粉葡萄糖苷酶 Amyloglucosidase (Aspergillus niger) 货号:E-AMGFR-500MG Megazyme中文站

淀粉葡萄糖苷酶

英文名:Amyloglucosidase (Aspergillus niger)

货号:E-AMGFR-500MG

规格:500mg

High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3
CAZY Family: GH15
From Aspergillus niger. Lyophilised powder. Electrophoretically homogenous.

High purity. Recommended for use in AOAC Method 997.08 (fructan).

Specific activity: ~ 35 U/mg (40°C, pH 4.5, soluble starch as substrate).
Stable at -20°C for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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木葡聚糖酶(GH74)(类芽孢sp。) Xyloglucanase (GH74) (Paenibacillus sp.) 货号:E-XGP74 Megazyme中文站

木葡聚糖酶(GH74)(类芽孢sp。)

英文名:Xyloglucanase (GH74) (Paenibacillus sp.)

货号:E-XGP74

规格:200 Units at 70°C

High purity recombinant exo-alpha-Sialidase (S. Typhimurium) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

EC 3.2.1.18
CAZy Family: GH33

Recombinant. From Salmonella typhimurium. In solution (Tris.HCl / NaCl / EDTA). Hydrolysis of unbranched, non-reducing terminal α-2,3-linked >> α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates.
Supplied at ~ 2,500 U/mL. 

Specific activity: ~ 750 U/mg (37oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid)

Store at 4oC.  

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二乙酰壳二糖 Diacetyl-Chiotobiose – 30mg 货号:O-CHI2 Megazyme中文站

二乙酰壳二糖

英文名:Diacetyl-Chiotobiose – 30mg

货号:O-CHI2

规格:30 mg

CAS: 35061-50-8
Molecular Formula: C16H28N2O11
Molecular Weight: 424.4
Purity: > 95%

High purity Diacetyl-chitobiose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Prepared from chitin.

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1,4-β-D- Cellotetraitol(硼氢化还原纤维四糖) 1,4-β-D-Cellotetraitol (borohydride reduced cellotetraose) 货号:O-CTERD Megazyme中文站

1,4-β-D- Cellotetraitol(硼氢化还原纤维四糖)

英文名:1,4-β-D-Cellotetraitol (borohydride reduced cellotetraose)

货号:O-CTERD

规格:50 mg

CAS: 5548-55-0
Molecular Formula: C24H44O21
Molecular Weight: 668.6
Purity: > 95%

High purity 1,4-β-D-Cellotetraitol (borohydride reduced cellotetraose) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Tetrasaccharides from hydrolysis of cellulose and borohydride reduced.

Store at room temperature.

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2-氯-4-硝基苯基β-(1,3:1,4)-glucopyranoside 2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotetraoside 货号:O-CNPBG4 Megazyme中文站

2-氯-4-硝基苯基β-(1,3:1,4)-glucopyranoside

英文名:2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotetraoside

货号:O-CNPBG4

规格:20 mg

Synonyms: 2-Chloro-4-nitrophenyl β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→3)-β-D-glucopyranoside
CAS: N/A
Molecular Formula: C30H44ClNO23
Molecular Weight: 822.1
Purity: > 97%

High purity 2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotetraoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement of lichenase or mixed linkage
β-glucanase (endo-1,3:1,4-β-D-glucanase) activity. As this substrate can also be hydrolysed by exo-acting β-glucanase/β-glucosidase enzymes, it is recommended only for the assay of pure lichenase solutions. The data sheet for the analogous trisaccharide substrate, 2-Chloro-4-nitrophenyl-β-(1,3:1,4)-glucotrioside (cat. no. O-CNPBG3), describes suitable assay conditions.

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裂解黄原[芽孢杆菌] Xanthan lyase (Bacillus sp.) 货号:E-XANLB Megazyme中文站

裂解黄原[芽孢杆菌]

英文名:Xanthan lyase (Bacillus sp.)

货号:E-XANLB

规格:20000 Units

High purity recombinant Xanthan lyase (Bacillus sp.) for use in research, biochemical enzyme assays and 
in vitro diagnostic analysis.

EC 4.2.2.12
CAZy Family: PL8

Recombinant. From Bacillus sp. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 3,000 U/mg (40oC, pH 6.0 on xanthan gum).

Store at 4oC. 

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多粘芽胞杆菌[芽胞杆菌] Xyloglucanase (GH5) (Paenibacillus sp.) 货号:E-XEGP Megazyme中文站

多粘芽胞杆菌[芽胞杆菌]

英文名:Xyloglucanase (GH5) (Paenibacillus sp.)

货号:E-XEGP

规格:3000 Units

High purity recombinant Xyloglucanase (GH5) (Paenibacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.151 
CAZy Family: GH5

Xyloglucan-specific endo-1,4-β-glucanase.

Recombinant from Paenibacillus sp. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 130 U/mg (40oC, pH 5.5 on tamarind xyloglucan).

Stability: > 2 years at 4oC. 

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车前草多糖解聚酶检测片剂 Psyllazyme – 200 Tablets 货号:T-PSYL-200T Megazyme中文站

车前草多糖解聚酶检测片剂

英文名:Psyllazyme – 200 Tablets

货号:T-PSYL-200T

规格:200 Tablets

High purity dyed and crosslinked Psyllazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of enzymes causing endo-depolymerisation of psyllium polysaccharide.Containing AZCL-psyllium polysaccharide.

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