BD DIFCO 灭活结核杆菌(M.Tuberculosis H37 Ra) 231141 上海代理商现货

特色

BD DIFCO 灭活结核杆菌(M.Tuberculosis H37 Ra) 231141 上海代理商现货

BD DIFCO灭活结核杆菌 231141 H37 RA 冻干粉

灭活结核杆菌(冻干粉) 结核杆菌 231141 BD DIFCO灭活结核杆菌231141

BD DIFCO 灭活结核杆菌(M.Tuberculosis H37 Ra) 231141
产品名称: BD DIFCO灭活结核杆菌 231141

英文名称: M Tuberculosis H37 Ra, Desiccatedr

产品货号: 231141

产品规格: 6*100MG

产品产地: 美国

产品商标: BD DIFCO

价    格: 询价

保存与运输: 2-8 摄氏度

产品名称: BD DIFCO灭活结核杆菌231141

英文名称: M Tuberculosis H37 Ra, Desiccatedr

产品货号: 231141

产品规格: 6*100MG

产品产地: 美国

产品商标: BD DIFCO

价    格: 询价

保存与运输: 2-8 摄氏度

harlan teklad官网代理商 标准胆固醇 TD.120097 TD.01383 TD.07841 动物饲料

特色

Standard, natural ingredient diets with cholesterol added are fed to induce hypercholesterolemia. Various levels of cholesterol, fat, and/or bile salts can be added to one of the numerous standard rodent diets stocked by Envigo Teklad. For many applications, adding these components to Envigo’s minimal-to-moderate phytoestrogen global rodent diets is recommended. Our minimal phytoestrogen global rodent diets are soybean meal free, limiting the effect of phytoestrogens on your research outcomes. Soybean meal, a common dietary source of phytoestrogens, has been shown to decrease aortic fatty streak development and modify plasma cholesterol, which may reduce the risk of developing atherosclerosis. Limiting dietary soybean meal may reduce confounding variables within your dietary-induced atherosclerosis model. Contact a nutritionist to discuss additional diet options.

Examples of minimal and moderate phytoestrogen rodent diets with added cholesterol:

  • TD.120097  1% cholesterol diet (2020 – minimal phytoestrogens)
  • TD.07841    2% cholesterol diet (2016 – minimal phytoestrogens)
  • TD.01383    2% cholesterol (2018 – Moderate phytoestrogens)

Research use:

Induce hypercholesterolemia in genetically-modified and wild type models without promoting obesity.

Key dietary features:

  • Standard, grain-based rodent diet
  • Minimal/moderate phytoestrogen diets recommended
  • Cholesterol (1 – 4%)

References:

  1. Belch, J.J., et al., Longitudinal assessment of endothelial function in the microvasculature of mice in-vivo. Microvasc Res, 2013. 85: p. 86-92.
  2. Hartvigsen, K., et al., A diet-induced hypercholesterolemic murine model to study atherogenesis without obesity and metabolic syndrome. Arterioscler Thromb Vasc Biol, 2007. 27(4): p. 878-85.

Rabbits, hamsters, and swine are common models of atherosclerosis. Contact a nutritionist for information and formula examples. See rabbit, swine and other speciesfor information and formula examples.

harlan teklad官网 动物饲料氯化钠缺乏 TD.90228 TD.08290   NaCl调节饲料

特色

NaCl调节(天然成分)

harlan teklad官网 动物饲料氯化钠高盐TD.92012 TD.92034 TD.96208 NaCl调节饲料

harlan teklad官网 动物饲料氯化钠缺乏 TD.90228 TD.08290   NaCl调节饲料

玉米、小麦、豆粕等天然成分的钠含量较低。因此,这些成分可以用来制造一种缺乏钠的基础饮食。在这一基础日粮中,可以添加各种量的氯化钠盐(NaCl),其他成分也可以稍加调整,以保持相对稳定的营养状况(除钠和氯化物外)。下面是一系列受欢迎的调整NaCl饮食的例子。欲了解更多信息,请与我们联系。

公式实例
添加NaCl(%) 大约。NA(%) 无染料 红*染料 橙*染料 蓝*染料
NA缺乏 0.01-0.02 TD.90228 TD.08290
可能的对照日粮:0.05%Na(0.1%NaCl)符合EST。最低钠需要量典型的啮齿动物日粮中含0.2%~0.4%Na(~0.5%-1%NaCl).
0.1 0.05 TD.94268 TD.07334
0.49 0.2 TD.96208 TD.110765
1 0.4 TD.90229
用于研究啮齿类动物摄入过量钠的影响的饮食。
2 0.8 TD.95078 TD.130345
4 1.6 TD.92034 TD.110078 TD.03095
8 3.2 TD.92012 TD.03142

*将这些水溶性食物染料加入天然成分的饮食中,可提供轻微的色泽。在较高的包合率下可以获得更强烈的颜色。

向营养师询问额外的盐浓度或颜色选择。

这些饮食是以谷物为基础的,没有动物来源的成分,并且背景钠含量约为。0.01-0.02%,背景氯含量约为0.01-0.02%.0.06-0.07%。日粮的营养成分为:蛋白质约19%,脂肪5%,粗纤维3%,钙0.86%,磷0.64%,钾0.72%,镁0.15%。

食盐调节后的饲料通常喂给Dahl盐敏感/耐盐(Rapp)近交系大鼠.这些老鼠传统饮食7034(0.12%NaCl)在最大安全生产设施内。

NaCl adjusted (natural ingredient) | Envigo

The sodium content of natural ingredients such as corn, wheat, and soybean meal is low. Thus, these ingredients can be used to create a base diet that is sodium deficient. To this base diet, various amounts of sodium chloride salt (NaCl) can be added, and other ingredients adjusted slightly to maintain a relatively constant nutrient profile (with the exception of sodium and chloride). Below are examples from a popular series of adjusted NaCl diets. Contact us for more information.

Formula examples
Added NaCl (%) Approx. Na (%) no dye red* dye orange* dye blue* dye
Na deficient 0.01-0.02 TD.90228 TD.08290
Possible control diets: 0.05% Na (0.1% NaCl) meets est. minimum Na requirement. Typical rodent diets contain 0.2%-0.4% Na (~0.5-1% NaCl).
0.1 0.05 TD.94268 TD.07334
0.49 0.2 TD.96208 TD.110765
1 0.4 TD.90229
Diets for studies that look at effects of excess sodium consumption in rodents.
2 0.8 TD.95078 TD.130345
4 1.6 TD.92034 TD.110078 TD.03095
8 3.2 TD.92012 TD.03142

* When added to natural ingredient diets, these water soluble food dyes offer a slight color tint. More intense color can be achieved at higher inclusion rates.

Ask a nutritionist about additional salt concentrations or color options.

These diets are grain-based, with no animal derived ingredients, and have a background sodium content of approx. 0.01-0.02% and a background chloride content of approx. 0.06-0.07%. The selected nutrient content of the diets are as follows: approximately 19% protein, 5% fat, 3% crude fiber, 0.86% Ca, 0.64% P, 0.72% K, and 0.15% Mg.

NaCl adjusted diets are often fed to dahl salt-sensitive/resistant (rapp) inbred rats. These rats are maintained on Teklad traditional diet 7034 (0.12% NaCl) within maximum security production facilities.

Research Diets官网代理商 动物饲料代理商

特色

Research Diets官网代理商 动物饲料代理商

美国research diets公司是一家专业生产实验室动物饲料的大型公司,包括脂肪热能的颗粒饲料及各种维生素缺乏饲料的专业供应商,Research Diets是生产动物实验用高脂肪饲料的先驱,可配制高达 60 kcal% 的高脂肪饲料,成份內含各式脂肪及碳水化合物,Research Diets长期陪伴实验室科研人员,以配制研究需要的饲料配方,可用于诱发肥胖病及糖尿病的特殊饲料如D12450B、D12451及 D12492, Research Diets拥有配方饲料种类超过15000种,常规高端饲料10多种如AIN-76A Western饲料等
食源性肥胖症饲料    

Research Diets动物造模饲料Research diets 食源性肥胖症饲料

肥胖是一种世界范围内的慢性疾病,是导致高血压、糖尿病、冠心病、心肌梗死等多种慢性病发生的主要危险因。

 

遗传和环境因素对肥胖症的发生起重要作用,膳食则是影响肥胖症的主要的环境因素之一。膳食诱导的肥胖动物模型普遍被用于肥胖发生机理和治疗方法的研究。

 

我们与世界顶级动物饲料供应商美国RDI合作,为客户提供配方可报告、可复制、可修改的纯化饲料,我们的饲料具有造模时间短、造模效果好、有效易复制等特点。

 

重点推荐饲料:D12492和D12451。

 推荐产品  货号  规格  生产厂商
60%Kcal High-Fat(DIO) Diets D12492 12.5kg 美国RDI
45%Kcal High-Fat(DIO) Diets D12451 12.5kg 美国RDI
10%Kcal High-Fat(DIO) 7% Sucrose Control Diets match D12492 D12450J 12.5kg 美国RDI
10%Kcal High-Fat(DIO) 17% Sucrose Control Diets match D12451 D12450H 12.5kg 美国RDI
10%Kcal High-Fat(DIO) 35% Sucrose Control Diets D12450B 12.5kg 美国RDI
10%Kcal High-Fat(DIO) No Sucrose Control Diets D12450K 12.5kg 美国RDI

上海金畔生物科技有限公司

一、公司简介


上海金畔生物科技有限公司提供生命科学研究领域系列产品,包括色谱标准品、生化试剂、诊断试剂、实验耗材和仪器。标准品和试剂包括分子质量测量标准品、固体标准品、液体标准品、修饰性PEG等。免疫诊断试剂包括酶、单克隆抗体、多克隆抗体和ELisa试剂盒等多种产品。耗材和仪器包括培养基、移液器、动物饲料、离心机等实验室常用仪器耗材。因此我们拥有广泛的客户群,覆盖药物分析、食品安全、聚合物分析、环境监测、物化工程和生物制药等领域,主要客户广泛分布于食品、制药、政府机构、第三方检测、环境测试机构、化工、科研、生物工程等行业。

二、公司名字和Logo

2.1公司名字的由来

“金畔”两个字代表金色湖畔的含义,因为公司发展和成长在于金色的黄埔江畔边,金畔生物致力于为中国科研提供便捷和高质量的科研试剂采购服务,为中国的科研发展做出一份贡献。

2.2 公司Logo

金畔生物Logo

公司Logo分为两部分,上部分为中文名字,下部分为英文名字。中文名字颜色为金色,代表金色湖畔的金色,英文名字部分颜色为蓝色,代表金色湖畔的碧蓝的湖水。

 

、公司产品主要的产品线

上海金畔生物科技有限公司提供的产品线包括三部分,生化试剂、诊断试剂和仪器耗材。

生化试剂包括固体标准品、液体标准品、溶液标准品、修饰性PEG(修饰性聚乙二醇)、表面张力测试剂、日立氨基酸分析试剂,PH缓冲溶液。

诊断试剂包括胎牛血清、酶、单克隆抗体、多克隆抗体、ELisa试剂盒、酶稳定剂、DNA/RNA保护试剂、DNA唾液采集器、细胞周期和细胞凋亡相关试剂。

仪器耗材包括细胞培养基、植物培养基、微生物培养基、动物饲料、仪器器和离心机等实验室常用仪器耗材。

、公司代理的品牌

金畔生物与众多国际一流品牌合作,并陆续成为他们在中国区的总代理或者一级代理。

标准试剂供应商:加拿大TRC、TLC,爱尔兰Reagecon、Megazyme,美国ChromaDex、Inorganic Ventures、Sigma-Aldrich、NIST、Sp2、Cayman,英国LGC、Ultra,日本和光WAKO、Shodex、JP、TCI,德国Witega、Dr.E、PSS 等,同时,还提供美国USP标准物质、欧洲药典标准物质EP等。

诊断试剂的供应商:Roche、ENZO、Lonza、Abnova、Abcom、Cayman、Biomatric、Lumiprobe、MP、Nussui、Laysan等

仪器耗材的供应:Labnet、Hampton、Corning,HTL,Millipore,Fortis,Reseach diets等

harlan teklad官网 动物饲料 TD.88137调整卡路里饮食 TD.1088545%脂肪KCAL饮食

特色

“Western” style diets are fed to genetically-modified cardiovascular models, such as Apoe and Ldlr deficient mice, to accelerate and enhance hypercholesterolemia and plaque formation and to elicit phenotypes commonly associated with metabolic syndrome. Within the atherogenic literature, a “Western” diet typically is described as a purified rodent diet with 20-23% milkfat/butterfat, 0.2% total cholesterol, and 34% sucrose by weight. TD.88137 is an example of a “Western” style diet that was originally designed to characterize and enhance atherosclerosis development in a newly generated Apoe-deficient mouse model. Contact us for more information about “Western” style diets, modifications, or possible control diets.

Examples:

  • TD.88137    Adjusted calories diet (42% from fat, 0.2% total cholesterol)
  • TD.10885    45% fat Kcal diet (0.2% total cholesterol)

Research use:

Accelerated hypercholesterolemia and plaque formation in genetically-modified models, such as Apoe and Ldlr deficient mice.

Used for diet-induced obesity in a variety of rodent models.

Key dietary features:

  • High Fat Diet (20-23% by weight; 40 – 45% kcal from fat)
  • Saturated fatty acids (SFA >60% of total fatty acids)
  • Milkfat/butterfat
  • Sucrose (34% by weight)
  • Cholesterol (0.2% total)

References:

  1. Febbraio, M., et al., Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice. J Clin Invest, 2000. 105(8): p. 1049-56.
  2. Huszar, D., et al., Increased LDL cholesterol and atherosclerosis in LDL receptor-deficient mice with attenuated expression of scavenger receptor B1. Arterioscler Thromb Vasc Biol, 2000. 20(4): p. 1068-73.
  3. Nakashima, Y., et al., ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb, 1994. 14(1): p. 133-40.
  4. Nakashima, Y., et al., Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the ApoE-deficient mouse. Arterioscler Thromb Vasc Biol, 1998. 18(5): p. 842-51.
  5. Plump, A.S., et al., Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells. Cell, 1992. 71(2): p. 343-53.
  6. Towler, D.A., et al., Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice. J Biol Chem, 1998. 273(46): p. 30427-34.
  7. Tsuchiya, K., et al., FoxOs integrate pleiotropic actions of insulin in vascular endothelium to protect mice from atherosclerosis. Cell Metab, 2012. 15(3): p. 372-81.

木聚糖酶[海栖热袍菌] endo-1,4-β-Xylanase (Thermotoga maritima) 货号:E-XYLATM Megazyme中文站

木聚糖酶[海栖热袍菌]

英文名:endo-1,4-β-Xylanase (Thermotoga maritima)

货号:E-XYLATM

规格:7500 Units at 80°C

High purity recombinant endo-1,4-beta-Xylanase (Thermotoga maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8 
CAZY Family: GH10

Recombinant. Catalytic domain of Xyn10A from Thermotoga maritima. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 115 U/mg (80oC, pH 5.0 on wheat arabinoxylan); ~ 22 U/mg (40oC, pH 5.0 on wheat arabinoxylan).

Store at 4oC.

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Hampton 96孔坐滴蛋白结晶板–Intelli-Plate

Hampton 96孔坐滴蛋白结晶板–Intelli-Plate

在生命科学领域,上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Hampton Intelli-Plate 96孔蛋白结晶板高品质的产品和服务,欢迎广大新老客户来电咨询。

Intelli-Plate 96 (Art Robbins Instruments)

Applications

  Sitting Drop Crystallization   用于蛋白坐滴结晶

Features  特征:

SBS microplate footprint & standard well spacing
Optically clear wells provide for superior crystal imaging
Well layout makes crystal harvesting easier
UV transmissible and low birefringence
2 well versions available in small and large drop well or two identical shallow drop wells
3 well versions are ideal for optimizing protein concentration, additive screens, drop ratio and combinatorial experiments
Rounded reservoir corners to prevent reservoir (MPD) creep

Description

Art Robbins Instruments has developed the ideal family of plates for crystallography applications: the Intelli-Plate. All versions of the Intelli-Plate have the SBS microplate footprint and standard well spacing and are ideal for manual or automated processing.

The Intelli-Plate 96-2 Low Profile (Art Robbins 102-0001-10 Hampton Research HR3-116 and HR3-117) is a low profile version of the Intelli-Plate 96-2 LVR (Low Volume Reservoir). Reservoir volume: 100 µl max. Drop well volume: 4 µl and 10 µl max. UV transmissible and low birefringence.

The CrystalMation Intelli-Plate 96-3 low-profile crystallization plate (Art Robbins 102-0001-13 Hampton Research HR3-118 and HR3-119) is a low profile version of the Intelli-Plate 96-3 LVR (Low Volume Reservoir). The plate is designed for sitting drop vapor diffusion crystallization experiments. It is constructed from optically clear, UV-transmissible, chemically resistant plastic with superior low birefringence. The low-profile construction allows for higher density in plate storage and imaging systems, maximizing your investment and valuable space. The plate is designed to be a substantial improvement in existing low-profile plates, allowing for superior sealing due to thicker well design and a flat sealing surface. It has been built to the SBS (Society for Biomolecular Screening) standard dimensions with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). It is compatible with automated instrumentation including the CrystalMation line of instruments from Rigaku. The plate features three identical sized sample drop wells per reservoir. The wells are concave depressions along the left side (Y-axis) of the plate and are located on the ledge above the adjacent, flat bottom reagent reservoir. Each well features a round bottom for easy crystal harvesting and can hold up to 1 µl of sample. The multiple well design allows for combinatorial experiments, complex co-crystallization experiments for ligand screening, or for drop concentration variation. The reagent reservoir is typically filled with 50 to 70 µl of reagent. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-2 LVR (Art Robbins 102-0001-00 Hampton Research HR3-143 and HR3-145) is a Low Volume Reservoir version of the original Intelli-Plate. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-3 LVR (Art Robbins 102-0001-03 Hampton Research HR3-183 and HR3-185) is a Low Volume Reservoir, 3 drop well version of the Intelli-Plate. Reservoir volume: 100 µl max. Typical reservoir volume 50 µl. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-2 Original crystallization plate (Art Robbins 102-0011-00 Hampton Research HR3-297 and HR3-299) is an optically clear plate for sitting drop vapor diffusion crystallization. All wells have standard 9 mm spacing to conform to SBS standards with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). This plate features two locations for sample per reservoir. The sample drop locations are located along the left side of the reagent reservoir, along the Y-axis of the plate. The sample wells are concave depressions on the ledge above the adjacent, flat bottom reagent reservoir. One sample well is located above the second sample well. The top sample well can hold 10 µl or less of sample. The lower sample well can hold 4 µl or less of sample. The reagent reservoir is typically filled with 100 µl of reagent and is capable of holding up to 300 µl of reagent. The sidewalls separating adjacent wells or reservoirs are 0.9 and 1.0 mm thick in order to offer a larger area for sealing the plate and a separation of the reservoirs. This plate is rigid, with virtually no torsional flex and is designed for either manual or automated pipetting. UV transmissible and low birefringence.

The Intelli-Plate Flat Shelf (Art Robbins 102-0001-01 Hampton Research HR8-171 and HR8-172) crystallization plate is a flat drop shelf version of the original Intelli-Plate. Reservoir volume: 300 µl max. No drop well. UV transmissible and low birefringence.

The Intelli-Plate 96-2 Shallow Well (Art Robbins 102-0001-20 Hampton Research HR3-163 and HR3-164) has two identically sized 2 µL reaction wells and a 140 µL screen reservoir. Each of the two identical well features a round bottom shallow well design for easy crystal harvesting and is arranged in an 8 x 12 array. This plate has low birefringence and is UV compatible. This plate is also available in a low profile height (Art Robbins 102-0001-21).

The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

The height of Intelli-Plate is 0.560 inches. The height of Intelli-Plate Low Profile is 0.315 inches (8.001 mm).

Intelli-Plate 96 (Art Robbins Instruments)

Intelli-Plate 96-3

CAT NO NAME DESCRIPTION
HR3-116 Intelli-Plate 96-2 Low Profile 20 plate case
HR3-117 Intelli-Plate 96-2 Low Profile 80 plate case
HR3-118 CrystalMation Intelli-Plate 96-3 low-profile 20 plate case
HR3-119 CrystalMation Intelli-Plate 96-3 low-profile 80 plate case
HR3-143 Intelli-Plate 96-2 LVR 10 plate case
HR3-145 Intelli-Plate 96-2 LVR 40 plate case
HR3-183 Intelli-Plate 96-3 LVR 10 plate case
HR3-185 Intelli-Plate 96-3 LVR 40 plate case
HR3-297 Intelli-Plate 96-2 Original 10 plate case
HR3-299 Intelli-Plate 96-2 Original 40 plate case
HR8-171 Intelli-Plate Flat Shelf 10 plate case
HR8-172 Intelli-Plate Flat Shelf 40 plate case
HR3-163 Intelli-Plate 96-2 Shallow Well 10 plate case
HR3-164 Intelli-Plate 96-2 Shallow Well 40 plate case
HR3-181 Intelli-Plate 96-2 Shallow Well Low Profile 20 plate case
HR3-182 Intelli-Plate 96-2 Shallow Well Low Profile 80 plate case

OECD培养基,浓缩液Ⅰ~Ⅳ


产品编号 产品名称 产品规格 产品等级 产品价格
158-03315 OECD Medium, Stock Solution
OECD培养基, 浓缩液
500mL 植物培养用
153-03321 OECD培地, 浓縮液Ⅱ(×1,000) 50mL 植物培养用
150-03331 OECD培地, 浓縮液Ⅲ(×1,000) 50mL 植物培养用
157-03341 OECD培地, 浓縮液Ⅳ(×1,000) 50mL 植物培养用

OECD培养基,浓缩液Ⅰ~ⅣOECD培养基, 浓缩液Ⅰ~Ⅳ

利用淡水藻类进行抑制生长试验



OECD培养基,浓缩液Ⅰ~Ⅳ

   WET(Whole Effluent Toxicity)是由美国研发的利用生物应答的水环境管理方法。本产品是WET法之一“淡水藻类生长抑制试验”的培养基配制用浓缩液。通过混合、稀释本产品,可以根据OECD TEST GUIDELINE No.201配制培养基。

 

OECD 培养基, 浓缩液Ⅰ~Ⅳ成分表


产品编号

规格

成分

浓度(mg/L) (原液)

浓度(mg/L) (稀释后)

158-03315

500 mL

OECD Medium,
     Stock Solution    I(×100)

×100

×1

NH4Cl

1,500.00

15.0000

MgCl2, 6H2O

1,200.00

12.0000

CaCl2, 2H2O

1,800.00

18.0000

MgSO4, 7H2O

1,500.00

15.0000

KH2PO4

 160.00

 1.6000

153-03321

50 mL

OECD Medium,
     Stock Solution Ⅱ(×1,000)

×1,000

×1

FeCl3, 6H2O

 64.00

0.0640

EDTA-Na2, 2H2O

100.00

0.1000

150-03331

50 mL

OECD Medium,
     Stock Solution Ⅲ(×1,000)

×1,000

×1

H3BO3

185.00

0.1850

MnCl2, 4H2O

415.00

0.4150

ZnCl2

  3.00

0.0030

CoCl2, 6H2O

  1.50

0.0015

CuCl2, 2H2O

  0.01

 0.00001

Na2MoO4,2H2O

  7.00

0.0070

157-03341

50 mL

OECD Medium,
     Stock Solution Ⅳ(×1,000)

×1,000

×1

NaHCO3

50,000.00

50.0000

 

◆特点

● 无需称量、溶解

● 已通过支原体实验

● 已进行0.2μm过滤灭菌

● 根据OECD Guideline No.201组成

 


◆OECD培养基的配制方法


通过将OECD培养基、浓缩液Ⅰ~Ⅳ混合、稀释,根据OECD TEST GUIDELINE No.201进行OECD培养基的配制。

配置1L的OECD培养基时

 

产品名称

需要量

OECD培养基, 浓缩液Ⅰ(×100)

10 mL

OECD培养基, 浓缩液Ⅱ(×1,000)

 1 mL

OECD培养基, 浓缩液Ⅲ(×1,000)

 1 mL

OECD培养基, 浓缩液Ⅳ(×1,000)

 1 mL

混合各浓缩液的需要量,稀释至1L

 

产品编号

产品名称

级别/厂家

规格

162-03652

Potassium Dichromate

试药特级

 25 g

196-15645

Sterile Water, Endotoxin Free

细胞培养

 500 mL

316-90101

Distilled Water, Deionized, Sterile

Nippongene

 100 mL

312-90103

100 mL×6

318-90105

 500 mL

012-11872

Agar (Powder)

植物培养

 25 g

016-11875

500 g

 

农残前处理柱(短柱)


产品编号 产品名称 产品规格 产品等级 产品价格
296-32651 Presep® -C Agri (Short)
农残前处理柱(短柱)
10 pcs×5 for Sample Pretreatment

农残前处理柱(短柱)

农残前处理柱(短柱)

Presep® -C Agri (Short)

  该固相萃取柱使用凝胶树脂(Styrene Divinylbenzene-Methacrylate)作为吸附材料,可迅速简便地进行农药残留分析的前处理。



◆特点


1、亲水性及疏水性聚合物凝胶可吸附水中存在的微量疏水性成分。

2、对难以回收的高极性及金属配位成分(Asulam•Oxine copper等)也具有极高的回收率。

3、使用柱处理器spe(减压浓缩装置),可在50分钟以内同时处理24个样本。还可使用

   Presep®-C Agri (Short),利用加压式定流泵进行回收、浓缩处理。

4、进行HPLC分析时,与Wakopak® WS Agri-9及专用洗提液组合使用,可迅速简便定量分析多种

   符合日本公定法规定的农药。

5、进行GC分析时,使用SGE公司的毛细管色谱柱,可进行选择性更高的分析。

Presep®-C Agri(Short)的回收率探讨(GC-MS分析条件)

固相萃取条件


色谱柱:Presep® -C Agri(Short)

柱活化:二氯甲烷5Ml,甲醇5mL,蒸馏水5mL

水样:在200mL蒸馏水中添加标准液100uL

固相吸附:以10-20mL/min的流速向柱进样

脱水:对柱通气10分钟

洗脱、脱水:将两个Presep®-C Na2SO4萃取柱连接至固相柱前端,用2.5mL二氯甲烷洗脱。

      然后拆下Presep®-C Agri(Short)后,用6mL二氯甲烷清洗Na2SO4柱,再与洗脱液混合。

浓缩:加入2mL己烷,吹氮浓缩至0.8mL (不得吹干)

分析样品:加入内标液100uL,用己烷定容至1mL(在分析结束前需冷藏保存)

标准液:10ug/mL丙酮溶液

内标液:萘嵌戊烷,苯并菲,2ug/mL己烷溶液为调整GC/MS分析中得灵敏度变化而添加

    两个Presep® -C Na2SO4脱水柱连接好后,用10mL二氯甲烷清洗后再使用。

 


GC分析条件


GC:HP5890 PACKARD SERIESE

GC/MS:AUTO mass JMS-AM150型(JEOL)

Column:100%Dimethyl Polysiloxane,0.25mm*30m,膜厚0.25um

Injector:250℃

升温:60℃(2min)-120℃(20℃/min)-300℃(10℃/min)-300℃(5min)

进样量:2.0uL

wako胡萝卜素标准品

wako胡萝卜素标准品

品牌 产品编号 产品名称 等级 规格 零售价(RMB) CAS No.
(生产商编号)
Wako 209-17281 Thapsigargin for Cellbiology 1 mg 1350 67526-95-8
毒胡萝卜素
Wako 205-17283 Thapsigargin for Cellbiology 5 mg 5390 67526-95-8
毒胡萝卜素
Wako 035-05531 β-Carotene Wako Special Grade 1g 400 7235-40-7
β-胡萝卜素
Wako 031-05533 β-Carotene Wako Special Grade 10g 860 7235-40-7
β-胡萝卜素
Biomol BML-PE180-0005 Thapsigargin 5mg 3840 67526-95-8
毒胡萝卜素
Alexis ALX-460-004-G005 β-Carotene 5g 810 7235-40-7
β-胡萝卜素
AdipoGen AG-CN2-0003-M010 Thapsigargin (high purity) 10 mg 5530 67526-95-8
毒胡萝卜素
AdipoGen AG-CN2-0003-M005 Thapsigargin (high purity) 5 mg 3220 67526-95-8
毒胡萝卜素
AdipoGen AG-CN2-0003-M001 Thapsigargin (high purity) 1 mg 810 67526-95-8
毒胡萝卜素
Wako 035-17981 α-Carotene Standard for High Performance

Liquid Chromatography

10mg 2860 7488-99-5
α-胡萝卜素标准品
Wako 032-17991 β-Carotene Standard for High Performance

Liquid Chromatography

10mg 920 7235-40-7
β-胡萝卜素标准品

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒 D-Mannose/D-Fructose/D-Glucose Assay kit 货号:K-MANGL Megazyme中文站

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒

英文名:D-Mannose/D-Fructose/D-Glucose Assay kit

货号:K-MANGL

规格:55 assays per kit

The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.

UV-method for the determination of D-Mannose, D-Fructose
and D-Glucosein foodstuffs, yeast cell preparations and
other materials

Principle:
(hexokinase)
(1) D-Mannose / D-fructose / D-glucose + ATP →
M-6-P / F-6-P / G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphomannose isomerase) (phosphoglucose isomerase)
(3) M-6-P ↔ F-6-P ↔ G-6-P

Kit size: 55 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 0.7 mg/L
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other
materials (e.g. biological cultures, samples, etc.)
Method recognition: Novel method

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

 Q1. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q5. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q6. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q7. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q8. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q9. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q10. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q11. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q12. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.