康宁corning 3473 PLATE,24WL,ULA,IND,PS,W/LID,S 24孔板 平底 超低吸附表面 PS(聚苯乙烯)材质 带盖 灭菌 单个包装 1个/包

康宁corning 3473 PLATE,24WL,ULA,IND,PS,W/LID,S
24孔板 平底 超低吸附表面 PS(聚苯乙烯)材质 带盖 灭菌 单个包装 1个/包 24包/箱 3485.47

Ames Test-Rat liver 9,000×g supernatant fraction


产品编号 产品名称 产品规格 产品等级 产品价格
636-00221 Ames Test-Rat liver 9,000×g supernatant fraction 2mL×10

S-9 大鼠肝匀浆/致突变性试验用(Ames试验用)

Ames Test-Rat liver 9,000×g supernatant fraction

◆「S-9」调配方法

  将雄性大鼠饲养与SPF环境下,养至7周大小(体重180g-200g)后,把诱导剂Phenobarbital(PB)和5, 6-Benzoflavone(BF)投与大鼠肝脏。

  PB是在剖杀前4天连续每天投注,BF则是剖杀前2天投注一次。然后无菌摘取大鼠肝脏,匀浆后9000xg离心,收集S-9上清。调配方法以Ames方法为准。



附上每个生产批次的生化特性检测值,如下:


生化特性

类型

检测项目

S-9

蛋白质含量

微粒体

(105,000×g)

 

蛋白质含量

Cytochrome   P-450含量

Cytochrome   β5含量

NADP-Cytochrome   reductase

Aniline   hydroxylase

附上每个生产批次的突变诱发性的生物活性检测值,如下:

 

生化活性

药剂

菌株

Benzo(α)pyrene

TA100
TA98
TA1537

2-Aminoanthracene

TA100
TA98

形态/保存

  到货后请直接保存于-80℃以下。

  干冰泡沫箱包装

◆相关链接


  Ames Test概要

4-硝基苯基-α-D-木糖 4-Nitrophenyl-α-D-xylopyranoside 货号:O-PNPAX Megazyme中文站

4-硝基苯基-α-D-木糖

英文名:4-Nitrophenyl-α-D-xylopyranoside

货号:O-PNPAX

规格:100 mg

Synonyms: p-Nitrophenyl-α-D-xylopyranoside, 
                   pNP-α-D-xylopyranoside
CAS: 10238-28-5
Molecular Formula: C11H13NO
7
Molecular Weight: 271.2
Purity: > 98%

High purity 4-Nitrophenyl-α-D-xylopyranoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for measurement of α-xylosidase activity.

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牛皮源去端肽胶原


产品编号 产品名称 产品规格 产品等级 产品价格
KOU-IPC-30 Atelocollagen, Bovine dermis, 3mg/mL 
去端肽胶原,牛皮来源,3 mg/mL
50ml/btl
KOU-IPC-50 Atelocollagen Bovine dermis 5mg/ml pH3.0 Steril 
去端肽胶原,牛皮来源,5 mg/mL
50ml/btl
KOU-IAC-30 Native collagen Bovine dermis 3mg/ml pH3.0 Steril 
天然胶原,牛皮来源,3 mg/mL
50ml/btl
KOU-IAC-50 Native collagen Bovine dermis 5mg/ml pH3.0 Steril 
天然胶原,牛皮来源,5 mg/mL
50ml/btl

  AteloCell™ Atelocollagen,Native collagen牛皮源去端肽胶原

◆原理

Cosmo Bio公司以先进的产品技术,优良的质量监控为广大客户提供用于细胞培养的胶原溶液。

Atelocollagen (端胶原)(IPC):胃蛋白溶解I型胶原溶液

Native collagen(天然胶原)(IAC):酸溶解I型胶原溶液

◆优点特色


所有产品均已灭菌。胶原蛋白从牛的真皮中提取,经过新颖的方法进行溶解纯化。在37℃,端胶原与天然胶原会形成纤维。

 

◆案例应用


胶原包被在培养皿上;细胞在胶原上三维培养



35mm胶原海绵培养皿

◆原理

胶原海绵细胞培养是以胶原蛋白为基础新开发出来的,可用于细胞3D培养。多孔胶原海绵是由牛跟腱提取的不溶性I型胶原蛋白制备而成。细胞可渗透入海绵,朝不同方向增殖。

◆优点特色

       所有产品均已灭菌 

◆案例应用

细胞在海绵上培养


产品编号

品名

包装

储存

KOU-CS-35

Collagen sponge for35mm culture dish Bovine tendon   Sterile

5pcs/box

RT

 


Atelocollagen, Permeable membrane for50mmculture dish

◆原理

从牛跟腱中提取的I型胶原蛋白制备的可渗透性胶原蛋白膜可用于单层或双层细胞培养。此胶原蛋白膜特别适用于研究在膜两侧培养的不同细胞间的分子相互作用,相互关系。膜的可渗透性允许氨基酸,蛋白质等小分子自由通过胶原蛋白膜,这对于细胞间的营养吸收,物质交换有重要作用。

◆优点特色


 两种不同细胞可在膜两侧生长,因此特别适合研究细胞或小分子相互间的作用。

 可渗透性膜非常适合细胞培养,也适合人工皮肤,人工胰腺等其它人工器官的培养。

产品编号

品名

规格

储存

KOU-MEN-01

Atelocollagen, Permeable membrane for50mmculture   dish

(33mm)Bovine   dermis   Sterile

5pcs/box

-20℃

 


 

Atelocollagen, Honeycomb sponge

◆原理

“蜂窝状”海绵的结构比较特殊,它的孔径大约200-400μm,密集地向一个方向排列,因此细胞可以顺着小孔渗透增殖。这样的结构,有利于海绵内部的细胞吸收营养物质,排出生物代谢物质。细胞在空腔内增殖,并形成统一的细胞团。

优势:从牛跟腱中提取胶原蛋白制备“蜂窝状”海绵,可以用胶原酶降解此海绵。

 牛皮源去端肽胶原牛皮源去端肽胶原Honeycomb sponge的电镜图

 

产品编号

品名

规格

储存

KOU-CSH-10

Atelocollagen Honeycomb sponge Bovine dermis

Size: about 3X3X2 mm/sponge   total surface area:2000cm2/g  weight per volume:   12-13mg/ml sterile

100mg/btl

室温


牛皮源去端肽胶原

AteloCell 细胞培养胶原.pdf

氨基酸混合标准溶液, B型


产品编号 产品名称 产品规格 产品等级 产品价格
012-08643 Amino Acids Mixture Standard Solution, Type B
氨基酸混合标准溶液, B型
1mLx5A for Amino Acid Automated Analysis
016-08641 Amino Acids Mixture Standard Solution, Type B
氨基酸混合标准溶液, B型
5mL for Amino Acid Automated Analysis

氨基酸混合标准溶液,B型   Amino Acids Mixture Standard Solution, Type B

 

  Wako生产的氨基酸混合标准可用于氨基酸自动分析仪或高效液相色谱(HPLC)进行氨基酸成分分析或测序。

组成成分:(每种氨基酸浓度2.50μmol/ml)


γ-氨基丁酸; 

乙醇胺; 

氯化铵; 

异羟赖氨酸;

L-鸟氨酸;

L-赖氨酸;

1-甲基-L-组氨酸;

L-组氨酸;

L-3-甲基组氨酸; 

L-鹅肌肽; 

L-肌肽;

L-精氨酸

 

相关产品

 

产品编号

产品名称

包装

011-14463 

Amino Acids Mixture Standard Solution, Type AN-2

1mL×5A

015-14461 

5mL

013-08391

Amino Acids Mixture Standard Solution, Type H

5mL

019-08393

1mL x5

六乙酰壳六糖 Hexaacetyl-Chitohexaose- 10mg 货号:O-CHI6 Megazyme中文站

六乙酰壳六糖

英文名:Hexaacetyl-Chitohexaose- 10mg

货号:O-CHI6

规格:10 mg

CAS: 38854-46-5
Molecular Formula: C48H80N6O31
Molecular Weight: 1237.2
Purity: > 90%

High purity Hexaacetyl-chitohexaose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Prepared from chitin.

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merck millipore,默克密理博 AG310-30MG | FLUORO-JADE® B

AG310-30MG | FLUORO-JADE® B

merck millipore,默克密理博 AG310-30MG | FLUORO-JADE® B

Description
Catalogue Number AG310-30MG
Trade Name
  • Fluoro-Jade
Description FLUORO-JADE® B
Overview N-terminal GST-tagged, recombinant human XIAP full length, expressed by baculovirus in Sf21 insect cells
Background Information Fluoro-JadeⓇ B is a polyanionic fluorescein derivative which sensitively and specifically binds to degenerating neurons. It is a dark red power that has a green iridescence with excitation peak at 480 nm and emission peak at 525 nm. The filter used for visualizing Fluoro-JadeⓇ B is a fluorescein/FITC filter. Fluoro-JadeⓇ B can be used on most tissue section types and thicknesses including frozen, vibratomed cryostat or paraffin-embedded sections from 3-50 µm. Fluoro-JadeⓇ B is faster and more reliable than older methods (e.g. suppressed silver) for the unequivocal qualitative detection and quantitative measurement of both and fine scale neuronal degeneration.

Product Information
Formulation
Presentation Solubility: Very soluble in water and bases, moderately soluble in alcohol and weak acids.

Applications
Key Applications
  • Immunofluorescence
Application Notes Appearance: Dark red powder with green iridescence.

Biological Information
Purity This layer chromatography using cellulose plates and a solvent system of n-propinol, water, and ammonium hydroxide (6:5:2) revealed the presence of two fluorescent isomers and two trace non-fluorescent bands. No amount of fluorescein or Fluoro-Jade was present
Molecular Weight 681

Physicochemical Information
Emission max.
  • 525 nm
Excitation max.
  • 480 nm
Sensitivity
  • Toxicity: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.

Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions The powder should be stored well sealed at room temperature, preferable in a dessicator, due to its hygroscopic nature. The liquid stock solution (0.01%) in distilled water can be stored at 2-8°C for up to 3 months. The working solution (0.0004%) in 0.1% acetic acid should be prepared fresh and not be stored or reused.

Packaging Information
Material Size 30 mg

α-(1-2,3,4,6)-D-甘露糖苷酶(海栖热袍) α-(1-2,3,4,6)-D-Mannosidase (Thermotoga maritima) 货号:E-AMANTM Megazyme中文站

α-(1-2,3,4,6)-D-甘露糖苷酶(海栖热袍)

英文名:α-(1-2,3,4,6)-D-Mannosidase (Thermotoga maritima)

货号:E-AMANTM

规格:5 Units at 90°C

High purity α-Mannosidase (Thermotoga maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.24
CAZy Family: GH38
CAS: 9025-42-7

alpha-mannosidase; alpha-D-Mannoside mannohydrolase, alpha-D-mannopyranosidase

Recombinant. From Thermotoga maritima.
In 3.2 M ammonium sulphate.
Supplied at ~ 71 U/mL. 

Specific activity: 4.5 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.0 and 90oC; ~ 0.2 U/mg at 40oC.

Stability: > 4 years 4oC.

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