日度归档：2024年3月2日

总亚硫酸盐检测试剂盒（酶法） Total Sulfite Assay Kit (Enzymatic) 货号：K-ETSULPH Megazyme中文站

发表于由

总亚硫酸盐检测试剂盒（酶法）

英文名：Total Sulfite Assay Kit (Enzymatic)

货号：K-ETSULPH

规格：50 assays (manual) / 500 assays (microplate)

The Total Sulfite (Enzymatic) test kit is a simple, highly specific and reliable, enzymatic method for the measurement and analysis of total sulfite (as SO2) in wines, beverages, foodstuffs and other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Total Sulfite (SO22-) in
beverages,foodstuffs and other materials

Principle:
(sulfite oxidase)
(1) SO32- + O2 + H2O ↔ SO42- + H2O2

(NADH – peroxidase)
(2) H2O2 + NADH + H+ → 2 H2O + NAD+

Kit size:                            50 assays (manual) / 500 (microplate) 
                                          / 588 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Total assay time:              ~ 30 min
Detection limit:                 0.3 mg/L
Application examples: 
Wine, beer, fruit juices, soft drinks, jam, fruit and vegetables,
pharmaceuticals and other materials
Method recognition:     
Methods based on this principle have been accepted by DIN, EN,
MEBAK and NMKL

Advantages

  • Very cost effective
  • All reagents stable for > 2 years during use
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

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HyClone培养基目录

发表于由

HyClone培养基目录
HyClone公司创立于1967年，总部位于美国犹他州洛根市，是细胞培养和相关产品的全球供应商。现在从属于世界著名的FISHER科学国际公司。
近40年来，HyClong公司一直是高品质胎牛血清（FBS）及其他血清产品行业的领导者，各种血清产品多年来稳居美国市场占有率第一名。自2000年起美国模式培养物保藏所（ATCC）对所有HyClone的血清实行免检采购。
HyClone还大批量生产各类经典的细胞培养基、不断推陈出新的无血清和无蛋白培养基，并且可以根据客户的批量型配方要求定制培养基。
HyClone液体培养基在使用的过程中要注意以下问题：
运输中要避光冷藏，瓶口保持向上，切勿剧烈振荡；
开瓶后不要长时间暴露在空气和强光下，以保持培养基的稳定性；
开瓶后zui好能在一周内用完，短时间内用不完可分装冷藏保存，但PH值会在随后的时间里慢慢变碱，属于正常现象，请放心使用；
所有液体培养基均经100纳米无菌过滤和出厂严格检测，除非需要，请不要私自进行二次过滤和任何形式的无菌检测。

普通液体培养基列表
描述 货号 包装 储存温度
BME培养基(Basal Medium Eagle)
EBSS*，含L-谷氨酰胺。 ● SH30157.01 500 ml 2-8°C
○ SH30157.02 1000 ml 2-8°C
DMEM培养基(Dulbecco’s Modified Eagle’s Medium)
高糖，含4.0 mM L-谷氨酰胺，不含丙酮酸钠。 ● SH30022.01B 500 ml 2-8°C
● SH30022.FS 6 x 500 ml 2-8°C
● SH30022.02B 1000 ml 2-8°C
● SH30022.LS 6 x 1000 ml 2-8°C
高糖，不含L-谷氨酰胺与丙酮酸钠。 ● SH30081.01 500 ml 2-8°C
● SH30081.FS 6 x 500 ml 2-8°C
● SH30081.02 1000 ml 2-8°C
● SH30081.LS 6 x 1000 ml 2-8°C
○ SH30081.03 20 L袋装 2-8°C
○ SH30081.04 10 L袋装 2-8°C
高糖，含4.0 mM L-谷氨酰胺、丙酮酸钠。 ●SH30243.01B 500 ml 2-8°C
●SH30243.FS 6 x 500 ml 2-8°C
●SH30243.02 1000 ml 2-8°C
●SH30243.LS 6 x 1000 ml 2-8°C
○SH30243.03 10 L袋装 2-8°C
○SH30243.04 20 L袋装 2-8°C
高糖，含L-谷氨酰胺、HEPES，不含丙酮酸钠。 ●SH30249.01 500 ml 2-8°C
●SH30249.02 1000 ml 2-8°C
高糖，不含钙、镁。 ●SH30262.01 500 ml 2-8°C
○SH30262.02 1000 ml 2-8°C
高糖，含L-谷氨酰胺，不含酚红与丙酮酸钠。 ●SH30284.01 500 ml 2-8°C
●SH30284.02 1000 ml 2-8°C
高糖，含丙酮酸钠，不含L-谷氨酰胺。 ●SH30285.01 500 ml 2-8°C
●SH30285.FS 6 x 500 ml 2-8°C
●SH30285.02 1000 ml 2-8°C
●SH30285.LS 6 x 1000 ml 2-8°C
○SH30285.03 20 L袋装 2-8°C
○SH30285.04 10 L袋装 2-8°C
高糖，改良型，不含酚红、丙酮酸钠、L-谷氨酰胺。 ○SH30585.01 500 ml 2-8°C
●SH30585.02 1000 ml 2-8°C
高糖，改良型，不含酚红、L-谷氨酰胺，含丙酮酸钠。 ●SH30604.01 500 ml 2-8°C
●SH30604.02 1000 ml 2-8°C
高糖，含丙酮酸钠，不含L-谷氨酰胺、甲硫氨酸、胱氨酸。 ○SH30606.01 500 ml 2-8°C
○SH30606.02 1000 ml 2-8°C
高度改良，含丙酮酸钠、25 mM HEPES，不含L-谷氨酰胺。 ○SH30563.01 500 ml 2-8°C
○SH30563.02 1000 ml 2-8°C
高糖，含丙酮酸钠，不含L-谷氨酰胺、磷酸盐。 ○SH30607.01 500 ml 2-8°C
低糖，含4.0 mM L-谷氨酰胺、110 mg丙酮酸钠。 ●SH30021.01B 500 ml 2-8°C
●SH30021.FS 6 x 500 ml 2-8°C
●SH30021.02 1000 ml 2-8°C
含2.50 mM L-谷氨酰胺、15 mM HEPES。 ●SH30023.01B 500 ml 2-8°C
●SH30023.FS 6 x 500 ml 2-8°C
●SH30023.02 1000 ml 2-8°C
含HEPES，不含L-谷氨酰胺。 ●SH30126.01 500 ml 2-8°C
●SH30126.FS 6 x 500 ml 2-8°C
○SH30126.02 1000 ml 2-8°C
含L-谷氨酰胺、HEPES、丙酮酸钠。 ●SH30261.01 500 ml 2-8°C
●SH30261.02 1000 ml 2-8°C
含L-谷氨酰胺，不含HEPES。 ●SH30271.01 500 ml 2-8°C
●SH30271.FS 6 x 500 ml 2-8°C
●SH30271.02 1000 ml 2-8°C
含L-谷氨酰胺，不含HEPES、酚红。 ●SH30272.01 500 ml 2-8°C
●SH30272.02 1000 ml 2-8°C
Ham”s培养基 (Ham’s Nutrient Mixture)
F10培养基，含L-谷氨酰胺。 ●SH30025.01 500 ml 2-8°C
○SH30025.02 1000 ml 2-8°C
F12培养基，含1.00 mM L-谷氨酰胺。, 1X ●SH30026.01B 500 ml 2-8°C
●SH30026.FS 6 x 500 ml 2-8°C
●SH30026.02 1000 ml 2-8°C
F12培养基, Kaighn”s改良型。 ●SH30526.01 500 ml 2-8°C
○SH30526.02 1000 ml 2-8°C
昆虫培养基 (Insect Media)
Grace”s无添加剂培养基 ○SH30610.01 500 ml 2-8°C
○SH30610.02 1000 ml 2-8°C
TNM-FH培养基 ●SH30280.02 500 ml 2-8°C
●SH30280.03 1000 ml 2-8°C
IMDM培养基 (Iscove”s Modified Dulbecco”s Medium)
含L-谷氨酰胺、HEPES，不含α-硫代甘油。 ●SH30228.01B 500 ml 2-8°C
●SH30228.FS 6 x 500 ml 2-8°C
●SH30228.02 1000 ml 2-8°C
不含L-谷氨酰胺。 ●SH30259.01 500 ml 2-8°C
●SH30259.02 1000 ml 2-8°C
Leibovitz培养基 (Leibovitz Medium)
L-15培养基，含2.05 mM L-谷氨酰胺。 ●SH30525.01 500 ml 2-8°C
●SH30525.02 1000 ml 2-8°C
M199培养基 (Medium 199)
EBSS*，含L-谷氨酰胺。 ●SH30253.01B 500 ml 2-8°C
○SH30253.FS 6 x 500 ml 2-8°C
●SH30253.02 1000 ml 2-8°C
HBSS*，含L-谷氨酰胺。 ○SH30233.01 100 ml 2-8°C
○SH30233.02 500 ml 2-8°C
HBSS*，含L-谷氨酰胺、L-氨基酸。 ○SH30330.01 500 ml 2-8°C
○SH30330.02 1000 ml 2-8°C
McCoy”s 5A培养基 (McCoy”s 5A Medium)
含L-谷氨酰胺。 ●SH30200.01 500 ml 2-8°C
●SH30200.FS 6 x 500 ml 2-8°C
●SH30200.02 1000 ml 2-8°C
含L-谷氨酰胺，不含酚红。 ●SH30270.01 500 ml 2-8°C
○SH30270.02 1000 ml 2-8°C
含L-谷氨酰胺、HEPES。 ○SH30602.01 500 ml 2-8°C
○SH30602.02 1000 ml 2-8°C
○SH30602.03 100 ml 2-8°C
MEM 培养基（Minimum Essential Medium）
EBSS*，含2.0 mM L-谷氨酰胺。 ●SH30024.01B 500 ml 2-8°C
●SH30024.FS 6 x 500 ml 2-8°C
●SH30024.02 1000 ml 2-8°C
●SH30024.LS 6 x 1000 ml 2-8°C
EBSS*，含L-谷氨酰胺，用于悬浮培养。 ○SH30235.01 500 ml 2-8°C
○SH30235.02 1000 ml 2-8°C
EBSS*，不含L-谷氨酰胺。 ●SH30244.01 500 ml 2-8°C
●SH30244.FS 6 x 500 ml 2-8°C
●SH30244.02 1000 ml 2-8°C
○SH30244.LS 6 x 1000 ml 2-8°C
α改良型，含L-谷氨酰胺、核糖核苷及脱氧核糖核苷。 ●SH30265.01B 500 ml 2-8°C
●SH30265.02 1000 ml 2-8°C
●SH30265.FS 6 x 500 ml 2-8°C
α改良型，不含L-谷氨酰胺、核糖核苷及脱氧核糖核苷。 ●SH30568.01 500 ml 2-8°C
○SH30568.FS 6 x 500 ml 2-8°C
○SH30568.02 1000 ml 2-8°C
Richter”s改良型，不含酚红。 ○SH30600.01 500 ml 2-8°C
○SH30600.02 1000 ml 2-8°C
Richter”s改良型，含L-谷氨酰胺、酚红。 ●SH30601.01 500 ml 2-8°C
○SH30601.02 1000 ml 2-8°C
用于悬浮培养，不含L-谷氨酰胺、氯化钙及镁。 ○SH30603.01 500 ml 2-8°C
○SH30603.02 1000 ml 2-8°C
RPMI 1640 培养基（RPMI 1640 Medium）
1X，含2.05 mM L-谷氨酰胺（同SH30091）。 ●SH30027.01 500 ml 2-8°C
●SH30027.FS 6 x 500 ml 2-8°C
●SH30027.02 1000 ml 2-8°C
●SH30027.LS 6 x 1000 ml 2-8°C
1X，含2.05 mM L-谷氨酰胺（同SH30027）。 ○SH30091.01 20 L袋装 2-8°C
○SH30091.02 10 L袋装 2-8°C
不含L-谷氨酰胺 ●SH30096.01 500 ml 2-8°C
●SH30096.FS 6 x 500 ml 2-8°C
●SH30096.02 1000 ml 2-8°C
○SH30096.LS 6 x 1000 ml 2-8°C
○SH30096.04 10 L袋装 2-8°C
○SH30096.05 20 L袋装 2-8°C
含HEPES，不含L-谷氨酰胺。 ○SH30203.05 500 ml 2-8°C
○SH30203.06 1000 ml 2-8°C
含L-谷氨酰胺、25 mM HEPES。 ●SH30255.01 500 ml 2-8°C
●SH30255.FS 6 x 500 ml 2-8°C
●SH30255.02 1000 ml 2-8°C
含L-谷氨酰胺、不含酚红。 ●SH30605.01 500 ml 2-8°C
○SH30605.02 1000 ml 2-8°C
含L-谷氨酰胺，不含硝酸钙。 ●SH30809.01B 500 ml 2-8°C
●SH30809.02B 1000 ml 2-8°C

货号标记说明：
“●” 表示常规生产产品。
“○” 表示按客户订单生产产品，有一定的最小定制量并需要一定时间。
“□” 表示未储存类项目，需要一段时间供给。
货号后标有“B”的 为赛默飞世尔生物化学制品（北京）有限公司生产，其他的为美国原产。
厚百商城提醒您：
所有液体培养基均经100纳米无菌过滤和出厂严格检测，除非需要，请不要私自进行二次过滤和任何形式的无菌检测。

ENDURO™迷你电泳电源

发表于由

品牌：美国Labnet

订货号：E0304-230V

产品介绍

      Labnet全新设计的EnduroTM 迷你电泳电源是DNA、RNA、蛋白电泳和印迹转移电泳的理想选择。

      迷你电泳电源相比同系列的250V/300V电源，身材更小巧更节省实验室空间，拥有更高的性价比，可额外购买配套的电源箱，使实验更高效并节约空间。

         ENDURO™迷你电泳电源      ENDURO™迷你电泳电源

      电源的微处理器采用反馈回路信号控制以保持操作参数的稳定。 程序选择包括恒定的电压输出和恒定的电流输出，定时或连续的运行。设定值采用轻触薄膜键完成，数字显示。一些安全参数已经被设计到软件中包括自动断路、空载探测、瞬间载荷变化探测和过电压保护。如果发生断电，可以设置机器重启。2个输出终端可以允许2对不同的凝胶电泳同时进行。

 

型号

Model 250V

Model 300V

Mini

货号

E0203-230V

E0303-230V

E0304

输出类型

恒定电压或恒定电流，自动短路保护

恒定电压或恒定电流，自动短路保护

恒定电压或恒定电流，自动短路保护

输出电压范围/增量

5-250V/1V

2-300V/1V

10-300V/1V

输出电流范围/增量

10-3,000mA/10mA

4-500mA/1mA

10-400mA/1mA

输出功率范围/增量

1-300W/1W

90W max/NA

60W

定时器

1min～99hr59min/连续

1min～99hr59min/连续

1-999min带报警/连续

编程功能

有，10步编程，可存20个程序

无

无

显示 

液晶显示屏

液晶显示屏

液晶数字显示屏

可连接电泳系统数量

4个

4个

2个

尺寸

19x25x8cm

19x25x8cm

14×19.1×8.4 cm

重量

2.5kg

2.2kg

1kg

输入电源

230V~50Hz

230V~50Hz

100-240V

 

  • 上海金畔生物科技有限公司

    文章号：558-558

Alpha淀粉酶[猪胰腺] α-Amylase (Porcine Pancreatic) 货号：E-PANAA-3G Megazyme中文站

发表于由

Alpha淀粉酶[猪胰腺]

英文名：α-Amylase (Porcine Pancreatic)

货号：E-PANAA-3G

规格：3g

High purity alpha-Amylase (Porcine Pancreatic) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.1 
CAZy Family: GH13

From porcine pancreas. Partially purified. Free flowing powder.

Specific activity: ca. 100,000 U/g (40oC, Ceralpha reagent).

Stable at -20oC for > 4 years.

For use in total dietary fiber (including resistant starch) assay procedure.

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harlan teklad官网动物饲料代理商 他莫昔芬饮食 Tamoxifen diets TD.130855

发表于由

harlan teklad官网动物饲料代理商

他莫昔芬饮食 harlan teklad官网动物饲料代理商 他莫昔芬饮食 Tamoxifen diets TD.130855

他莫昔芬激活Creer的饮食

有几篇文献引用了含有他莫昔芬的饮食对CRE模型在雌激素受体突变的控制下的应用。恩维戈特克乐饮食使各种含他莫昔芬的饮食定制，以满足您的研究需要。我们还制作了各种各样的含多西环素饮食对于被监管的系统。如果你有任何问题，请咨询营养师。

枸橼酸他莫昔芬和他莫昔芬

他莫昔芬作为他莫昔芬-蔗糖混合物添加到饮食中.鼓励用户使用库存供应。然而，泰克乐将继续与客户准备的混合。如果您选择此选项，请咨询营养师，以获得有关配制和发送混合物的更多信息。

鼓励用户从以下库存选项中进行选择，以便在几天内随时可供装运。

特克乐提供USP级三苯氧胺和枸橼酸他莫昔芬的饮食。
他莫昔芬
每公斤饲料毫克		 日他莫昔芬
每公斤体重毫克1		 饮食编码
(特克勒德供应他莫昔芬)
    天然色 红色
250 40 TD.130855 TD.130856
500 80 TD.130857 TD.130858
400柠檬酸盐2 40 TD.130859 TD.1308603

1假设体重20-25克，摄入量3-4克。
2枸橼酸他莫昔芬为66%他莫昔芬
3在欧洲称为TAM 400/Creer(TD.55125)

请为辐照饮食增加5-10天的准备时间；库存和定制产品至少需要3公斤订单。

这些饲料都有他莫昔芬与~5%蔗糖预混合，作为一种适口性增强剂；然而，饲料厌恶仍有可能发生。如果摄入是一个问题，请参阅下面的建议。

TeculadGlobal2016-这些例子中的基础饮食-仅仅是少数几种植物雌激素饮食中的一种。咨询营养学家关于其他基础饮食的使用。

折叠所有标签

  • 最低订货量为3公斤，足以喂养20只小鼠一个月。
  • 储存冷冻饮食，并计划在六个月内使用。
  • 典型的准备时间为两周(如果经过辐照，则为四周)
  • 辐照(可选)20 kGy的最低剂量(必须按顺序要求)
  • 订购：请致电800.483.5523或在线启动订单

计划：

  • 最初减少食物摄入量和减肥
  • 日常观察动物的耐受性-咨询您的IACUC监测指标。
  • 必要时进行干预

如果摄入似乎是一个问题：

  • 通过进行他莫昔芬给药试验，确定低剂量对你的模型是否有效。
  • 用含他莫昔芬颗粒与常规饲料颗粒混合逐渐驯服小鼠
  • 把食物弄湿，然后放在笼子里的盘子里(需要每天更换)。
  • 平日喂饲他莫昔芬饮食，周末有规律饮食。
  • 每隔一周(三苯氧胺饮食两周，休息一周)进行更长时间的治疗
  • 他莫昔芬是一种选择性雌激素受体调节剂(SERM)，这意味着它可以抑制雌激素的作用或具有促进雌激素的作用。
  • 人体治疗效果的一般剂量为20毫克/天。用于啮齿动物的他莫昔芬饮食通常含有~1至2毫克/粒。
  • 当处理饮食时，可以使用典型的实验室预防措施，如实验室外套、手套和面罩，尽量减少接触他莫昔芬的事故。
  • 您的化学品安全部门应联系，以获得更多的机构特定的指导方针，以处理和处置含有他莫昔芬的饮食。
  • 治疗时间从一周到两周不等。1,2,5,6,7,8一至两个月3,4,8
  • 纯他莫昔芬1,5,8和枸橼酸他莫昔芬1,2,3,4,6,7都是有效的
  • 通常他莫昔芬剂量为每天40-80毫克/公斤体重。
  • 纯他莫昔芬的典型包合物为250 mg。8或500毫克1,5枸橼酸他莫昔芬为每公斤400毫克。1,2,3,4,6,7
  • 报告的初始重量损失为10%。1,2,4,7,8，与食物摄入量减少有关2
  • 恢复正常饮食后体重的恢复可能会因基因失活而受损。2,7
  1. 安德森·KB，Winer LH，Mork HK，Molkentin JD，Jaisser F.2010。他莫昔芬诱导CRE介导的小鼠心脏基因断裂的给药途径和剂量。 转基因RES 19：715-725。
  2. 蒋介明，凌杰，郑永赫，价格DL，Aja SM，Wong PC.2010年。TDP-43的缺失下调了与肥胖相关的基因Tbc1d1，并改变了身体的脂肪代谢。 美国Acad Sci美国：16320-16324。
  3. Kardakaris R.，Gareus R.，Xandomlea S，Pasparakis M.2011。内皮和巨噬细胞特异性P38αMAPK缺乏不影响ApoE-/-小鼠动脉粥样硬化的发病机制。 PLOSOne 6：e21055。
  4. [2]Kiermayer C，Conrad M，Schneider M，Schmidt J，Brielmeier M.枸橼酸他莫昔芬口服对小鼠心脏时空基因灭活的优化。 创世纪45：11-16。
  5. Koitabashi N，Bedja D，Zaiman AL，Pinto Ym，Zhang M，Gabrielson KL，Takimoto E，Kass DA。2009年。心肌细胞靶向他莫昔芬诱导MerCreMer基因缺失模型中短暂性心肌病的避免。 中国保监会第105：12-15号决议
  6. Kratsios P，Catela C，Salimova E，Huth M，Berno V，Rosenthal N，Morkioti F.2009。细胞自主Notch信号在胚胎和成年心脏心肌细胞中的独特作用。 PCirc第106：559-572号决议。
  7. Miro-Murillo M，Elorza A，Soro-ArNaiz I，Albacete-Albacete L，Ordonz A，Balsa E，Vara-Vega A，Vazquez S，Fuertes E，Fernandez-Criado C，Landazuri MO，Aragones J.2011。急性VHL基因失活可诱导心脏HIF依赖的促红细胞生成素基因表达. PLOS One 6：E 22589。
  8. Welle S，Burgess K，Thornton CA，Tawil R.2009。成熟小鼠肌生长抑素耗竭程度与肌肉生长的关系。 李玉玲.医学杂志.1997(4)：E 935-40.

Tamoxifen diets

Diets with tamoxifen activate CreER

Several references cite use of tamoxifen-containing diets for models with Cre under the control of a mutated estrogen receptor. Envigo Teklad diets makes a variety of tamoxifen-containing diets customized to meet your research needs. We also make a variety of doxycycline-containing diets for tet regulated systems. If you have any questions, don’t hesitate to consult a nutritionist.

Teklad stocks tamoxifen and tamoxifen citrate

Tamoxifen is added to the diet as a tamoxifen-sucrose mixture. Users are encouraged to use the stocked supply. However, Teklad will continue to work with customer prepared mixes. If you choose this option, please consult a nutritionist for additional information about preparing and sending the mixture.

Users are encouraged to select from the stocked options below supply readily available to ship within days.

Teklad offers diet with USP grade tamoxifen and tamoxifen citrate
Tamoxifen
mg per kg diet		 Daily tamoxifen
mg per kg body weight1		 Diet code
(Teklad supplied tamoxifen)
    Natural colored Red
250 40 TD.130855 TD.130856
500 80 TD.130857 TD.130858
400 citrate2 40 TD.130859 TD.1308603

1 Assumes 20-25 g body weight and three-four g intake
2 Tamoxifen citrate is 66% tamoxifen
3 Referred to as TAM400/CreER in Europe (TD.55125)

Please add 5-10 day lead time for irradiated diets; minimum 3 kg orders are required for stocked and customized items.

These diets all have tamoxifen premixed with ~five percent sucrose as a palatability enhancer; however, feed aversion may still occur. If intake is a problem, see below for advice.

Teklad Global 2016 — base diet in these examples — is only one of several minimal phytoestrogen diets Teklad produces. Consult a nutritionist about use of other base diets.

COLLAPSE ALL TABS

  • Minimum order quantity is three kg, sufficient for feeding ~20 mice for one month
  • Store diet refrigerated and plan to use within six months
  • Typical lead time is two weeks (four weeks if irradiated)
  • Irradiation (optional) minimal dose of 20 kGy (Must be requested at time of order)
  • To place an order: contact Teklad Customer Service at 800.483.5523 or initiate order online

Plan for:

  • Initial reduction in food intake and weight loss
  • Daily observation of animal tolerance — consult your IACUC for monitoring metrics
  • Intervene when necessary

If intake seems to be a problem:

  • Determine if a lower dose is effective for your model by conducting a tamoxifen dose feeding trial
  • Gradually acclimate mice by mixing tamoxifen-containing pellets with regular feed pellets
  • Wet the food, then place in a dish inside the cage (requires daily replacement)
  • Feed tamoxifen diet on weekdays, regular diet on weekends
  • Alternate weeks (two weeks on tamoxifen diet, one week off) for longer treatments
  • Tamoxifen is a selective estrogen receptor modulator (SERM) meaning it can repress actions of estrogen or have pro-estrogen effects
  • Usual dose for therapeutic effects in humans is 20 mg/day. Tamoxifen diets for rodents typically contain ~one to two mg/pellet
  • Accidental tamoxifen exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
  • Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of tamoxifen containing diets.
  • Length of treatment varies from one to two weeks1,2,5,6,7,8 to one to two months3,4,8
  • Pure tamoxifen1,5,8 and tamoxifen citrate1,2,3,4,6,7 are both effective
  • Usual tamoxifen doses are ~40-80 mg per kg body weight per day
  • Typical inclusion for pure tamoxifen is 250 mg8 or 500 mg1,5 and for tamoxifen citrate is 400 mg per kg diet1,2,3,4,6,7
  • Initial weight loss of ten % is reported1,2,4,7,8, associated with reduced food intake2
  • Subsequent recovery of body weight after returning to regular diet may be compromised by gene inactivation2,7
  1. Andersson KB, Winer LH, Mork HK, Molkentin JD, Jaisser F. 2010. Tamoxifen administration routes and dosage for inducible Cre-mediated gene disruption in mouse hearts. Transgenic Res 19:715-725.
  2. Chiang PM, Ling J, Jeong YH, Price DL, Aja SM, Wong PC. 2010. Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism. Proc Natl Acad Sci USA 107:16320-16324.
  3. Kardakaris R, Gareus R, Xanthoulea S, Pasparakis M. 2011. Endothelial and macrophage-specific deficiency of P38alpha MAPK does not affect the pathogenesis of atherosclerosis in ApoE-/- mice. PLoS One 6:e21055.
  4. Kiermayer C, Conrad M, Schneider M, Schmidt J, Brielmeier M. 2007. Optimization of spatiotemporal gene inactivation in mouse heart by oral application of tamoxifen citrate. Genesis 45:11-16.
  5. Koitabashi N, Bedja D, Zaiman AL, Pinto YM, Zhang M, Gabrielson KL, Takimoto E, Kass DA. 2009. Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models. Circ Res 105:12-15.
  6. Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F. 2009. Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart. PCirc Res 106:559-572.
  7. Miro-Murillo M, Elorza A, Soro-Arnaiz I, Albacete-Albacete L, Ordonez A, Balsa E, Vara-Vega A, Vazquez S, Fuertes E, Fernandez-Criado C, Landazuri MO, Aragones J. 2011. Acute Vhl gene inactivation induces cardiac HIF-dependent erythropoietin gene expression. PLoS One 6:e22589.
  8. Welle S, Burgess K, Thornton CA, Tawil R. 2009. Relation between extent of myostatin depletion and muscle growth in mature mice. Am J Physiol Endocrinol Metab.Oct;297(4):E935-40.

labnet官网代理商 5695 Optipette20~200ul系列移液器    

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labnet官网代理商 5695 Optipette20~200ul系列移液器

Enduro 15.10 & 15.15

E1015-10-GT             Gel Tray 15 x 10cm, UVT, for 15 cm gel system       804

E1015-15-GT             Gel Tray 15 x 15cm, UVT, for 15 cm gel system       804

E1015-CD                   Casting Dams, for use with 15 cm gel system, set of 2  804

E1115-35-1.5            35 well comb for use with 15cm gel system, 1.5mm thick      638

E1115-30MC-1.5               30 well MC comb for use with 15cm gel system, 1.5mm thick       638

E1115-30MC-1                   30 well MC comb for use with 15cm gel system, 1mm thick  638

E1115-28MC-1.5               28 well MC comb for use with 15cm gel system, 1.5mm thick       638

E1115-28MC-1                   28 well MC comb for use with 15cm gel system, 1mm thick  638

E1115-28MC-0.75             28 well MC comb for use with 15cm gel system, 0.75mm thick     638

E1115-20-2                20 well comb for use with 15cm gel system, 2mm thick         638

E1115-20-1.5            20 well comb for use with 15cm gel system, 1.5mm thick      638

E1115-20-1                20 well comb for use with 15cm gel system, 1mm thick         638

E1115-20-0.75          20 well comb for use with 15cm gel system, 0.75mm thick   638

E1115-18MC-1.5               18 well MC comb for use with 15cm gel system, 1.5 mm thick      638

E1115-18MC-1                   18 well MC comb for use with 15cm gel system, 1mm thick  638

E1115-16-1                16 well comb for use with 15cm gel system, 1mm thick         638

E1115-16MC-2                   16 well MC comb for use with 15cm gel system, 2.0mm thick       638

E1115-16MC-1.5               16 well MC comb for use with 15cm gel system, 1.5mm thick       638

E1115-16MC-1                   16 well MC comb for use with 15cm gel system, 1mm thick  638

E1115-14MC-2                   14 well MC comb for use with 15cm gel system, 2mm thick  638

E1115-14MC-1.5               14 well MC comb for use with 15cm gel system, 1.5mm thick       638

E1115-12-1.5            12 well comb for use with 15cm gel system, 1.5mm thick      638

E1115-12-1                12 well comb for use with 15cm gel system, 1mm thick         638

E1115-10-1.5            10 well comb for use with 15cm gel system, 1.5mm thick      638

E1115-10-1                10 well comb for use with 15cm gel system, 1mm thick         638

E1115-8-1.5               8 well comb for use with 15cm gel system.  1.5 mm thick   638

E1115-8-1                  8 well comb for use with 15cm gel system, 1mm thick  638

Enduro 20.20

E1020-20-GT             Gel Tray 20 x 20 cm, UVT, for 20 cm gel system      1095

E1020-CD                   Casting Dams, for use with 20 cm gel system,  set of 2       1095

E1120-50-2                50 well comb for use with 20cm gel system, 2mm thick         762

E1120-40MC-2                   40 well MC comb for use with 20cm gel system, 2mm thick  762

E1120-40MC-1.5               40 well MC comb for use with 20cm gel system, 1.5 mm thick      762

E1120-40MC-1                   40 well MC comb for use with 20cm gel system, 1 mm thick 762

E1120-30-1                30 well comb for use with 20cm gel system, 1mm thick         762

E1120-25-1                25 well comb for use with 20cm gel system, 1mm thick         762

E1120-20MC-1.5               20 well MC comb for use with 20cm gel system, 1.5mm thick       762

E1120-20MC-1                   20 well MC comb  for use with 20cm gel system, 1mm thick       762

E1120-20MC-0.75             20 well comb for use with 20cm gel system, 0.75mm thick   762

E1120-16-0.75          16 well comb for use with 20cm gel system, 0.75 mm thick  762

 

ENDURO MOLECULAR WEIGHT MARKERS

R1000-100bp            Enduro Molecular Weight Markers, 100 bp DNA ladder, 0.1 ug/µl, 500 µl vial       740

R1000-1Kb                 Enduro Molecular Weight Markers, 1Kb DNA ladder, 0.1 ug/µl, 500 µl vial   725

 

Electrophoresis – mini gel system

E0160-230V               Enduro Gel XL, with standard casting set (E0168), gel tank with safety lid and power supply.  To specifiy cord type add -EU or UK to the end of the product number    7818

E0161                Large gel tray set (12.5 x 12cm) pack of 2       340

E0162                Small gel tray set (12.5 x 6 cm), pack of 2        340

E0163                Micro gel tray set (6 x 6 cm), pack of 4   476

E0164                Large comb set, 14/28 teeth, reversible, pk of 2    476

E0165                Small comb set, 5/8 teeth, reversible, pack of 2     476

E0166                Micro casting set for four gels, includes casting stand, four 6 x 6 cm gel trays and two combs    1632

E0167                Casting Stand, for all gel sizes       476

E0168                Standard casting set, includes 1 large and 2 small gel trays, 4 large combs and a casting stand 1700

 

Electrophoresis – plate sealers

PS1000-230V             NEW AccuSeal Semi Automated Plate sealer, includes adapters for standard and deepwell microplates, 230V, includes EU and UK power cords     59754

PS1000-ADAPT                   NEW AccuSeal Adapter for sealing assay plates     2570

PS1000-PCR               NEW AccuSeal Adapter for sealing PCR and 384 well plates  3930

 

 

 

Shakers – vortex mixer

S0200-230V               VX-200 Vortex Mixer with CombiCup head, 230V,(add -EU and -UK to specify cord type)      2830

S0200-21           Optional head attachment for 24 x 1.5/2.0 ml tubes, 24 x 0.5 ml tubes and 32 x 0.2 ml tubes (or 4 tube strips) 436

S0200-22           Optional head attachment for 1 microplate or 64 x 0.2 ml tubes or 8 x 0.2 ml tube strips    531

S0200-23           Optional head attachment for 8 x 15 ml and 8 x 12/13 mm diameter tubes  531

S0200-24           Optional head attachment for 6 x 50 ml tubes        531

S0200-25           Optional head attachment for 12 x 1.5/2.0 ml tubes, held horizontally 1020

S0200-26           Optional head attachment for 4 x 15 ml tubes, held horizontally   1020

S0200-27           Optional head attachment for 2 x 50 ml tubes, held horizontally   1020

 

 

Shakers- vortexing

S2020-M60-B            Orbit M60 Digital Shaker with platform for 60 microtubes, 120V  13919

S2020-M60-B-230V          Orbit M60 Digital Shaker with platform for 60 microtubes, 230V,  Includes EU and UK power cords 13919

S2020-R             Extra workstation/transfer rack for Orbit M60 Digital Shaker        1061

C1205                Individual adapters for 0.5/0.6 ml tubes, pk of 6    525

C1206                Individual adapters for 0.4/0.25 ml tubes, pk of 6  525

C1222                Individual adapters for 0.2ml thermal cycling tubes, pk of 6  525

S2020-P2-B                Orbit P2 Digital Shaker with platform for 2 microplates, 120V       12520

S2020-P2-B-230V              Orbit P2 Digital Shaker with platform for 2 microplates, 230V, Includes EU and UK power cords 12520

S2020-P4-B                Orbit P4 Digital Shaker with platform for 4 microplates, 120V       13182

S2020-P4-B-230V              Orbit P4 Digital Shaker with platform for 4 microplates, 230V, Includes EU and UK power cords 13182

 

S2030-300-B              Orbit 300 Multipurpose Digital Vortexer, without platform, 120V 15465

S2030-300-B-230V            Orbit 300 Multipurpose Digital Vortexer, without platform, 230V,  Includes EU and UK power cords         15465

S2030-10           Platform for four microplates 2062

S2030-12           Flat platform ( 30 x 30 cm) with nonslip rubber mat        2136

S2030-13           Spring loaded clamp platform for flasks, botttles, tube racks, etc.         3682

 

Shakers – incubator

S2056A              VorTemp 56 Shaking Incubator for microtubes and microplates, 120V  35791

S2056A-230V            VorTemp 56 Shaking Incubator for microtubes and microplates, 230V, Included EU and UK power cords    35791

S2050A              VorTemp 1550 Shaking incubator for microtubes and microplates, 120V       40137

S2050A-230V            VorTemp 1550 Shaking incubator for microtubes and microplates, 230V, Includes EU and UK power cords         40137

C1205                Individual adapters for 0.5/0.6 ml tubes, pk of 6    525

C1206                Individual adapters for 0.4/0.25 ml tubes, pk of 6  525

C1222                Individual adapters for 0.2ml thermal cycling tubes, pk of 6  525

 

Shakers – orbital

S2030-1000-B           Orbit 1000 Digital Shaker without platform, 120V 18559

S2030-1000-B-230V                   Orbit 1000 Digital Shaker without platform, 230V includes EU and UK power cords     18559

S2031-12           Flat platform with non-slip rubber mat   1988

S2031-12D                 Two stacked platforms (30 x 30 cm) with nonslip rubber mat         3240

S2031-13           Spring loaded clamp platform for flasks, bottles, tube racks, etc. 3682

S2031-18           Flask clamp platform without clamps (see catalog for flask capacities)          3240

S2040-25           Clamp for 25 ml Erlenmeyer Flask           442

S2040-50           Clamp for 50 ml Erlenmeyer Flask           560

S2040-85           Clamp for 125 ml Erlenmeyer Flask          531

S2040-99           Clamp for 250 ml Erlenmeyer Flask          560

S2040-09           Clamp for 500 ml Erlenmeyer Flask        633

S2040-01           Clamp for 1 L Erlenmeyer Flask      693

 

S2040-1900               Orbit 1900 Digital Shaker, without platform, 120V          30194

S2040-1900-230V              Orbit 1900 Digital Shaker, without platform, 230V,  Includes EU and UK power cords         30194

S2040-12           Flat platform with nonslip mat (40x50cm)       3461

S2040-15           Universal bar clamp platform for holding a variety of containers   6039

S2040-18           Flask clamp platform without clamps  (see catalog for flask capacities)      4345

S2040-25           Clamp for 25 ml Erlenmeyer flask 442

S2040-50           Clamp for 50 ml Erlenmeyer Flask   560

S2040-85           Clamp for 125 ml Erlenmeyer Flask          531

S2040-99           Clamp for 250 ml Erlenmeyer Flask        560

S2040-09           Clamp for 500 ml Erlenmeyer Flask        633

S2040-01           Clamp for 1 L Erlenmeyer Flask      693

S2040-02           Clamp for 2 L Erlenmeyer flask (      810

 

S2030-LS-B                 Orbit LS Low Speed Orbital Shaker with flat platform, 120V   13182

S2030-LS-B-230V               Orbit LS Low Speed Orbital Shaker with flat platform, 230V, includes EU and UK power cords      13182

 

Shakers – reciprocal

S2030-RC-B               Pro 30 Reciprocal Shaker without platform, 120V  12078

S2030-RC-B-230V              Pro 30 Reciprocal Shaker without platform, 230V, includes EU and UK power cords     12078

S2031-12           Flat platform with non-slip rubber mat Shaker        1988

S2031-12D                 Two stacked platforms (30 x 30 cm) with nonslip rubber mat         3240

S2031-13           Spring loaded platform for flasks, bottle or tube racks   3682

S2031-18           Flask clamp platform without clamps  (see catalog for flask capacities)      3240

S2040-85           Flask clamp, 125 ml (max 15)  531

S2040-99           Flask clamp, 250 ml (max 9)    560

S2040-09           Flask clamp, 500 ml (max 5)    633

S2040-01           Clamp for 1 L flask (max 4)       693

 

Shakers- rotating

H5000-230V              LabRoller Rotator (order rotisserie/carousel separately),230V, Includes EU and UK power cords        12372

H5100-230V              Compact LabRoller Rotator (order carousel separately),230V, Includes EU and UK power cords          11600

H1200-RA                  Rocking platform, fits both H5000 and H5100         2937

H1212-35A                Rotisserie for 12 of 300 x 35 mm diameter bottles, 24 of 150 x 35 mm diameter bottles or 24 of 50 ml tubes horizonal.  Fits H5000 only    4963

H1212-40VA              Rotisserie for 12 x 50ml tubes held vertically, fits H5000 only.       6322

H1264-VA                   Rotisserie for 64 x 1.5ml, or 32 x 15 ml vertical, fits H5000 only    8430

H1264-HA                  Rotisserie for 64 x 1.5 ml or 32 x 15 ml, horizontal, fits H5000 only       8294

H5108-50A                Carousel for 8 x 50 ml tubes, vertical, fits both H5000 and H5100         1666

H5116-15A                Carousel for 16 x 15ml tubes, vertical, fits H5000 and H5100, Call Labnet for 3 or 4 carousels on H5000    2175

H5132-HA                  Carousel for 32 x 1.5 ml microtubes, horizontal, fits both H5000 and H5100         2651

H5132-VA                   Carousel for 32 x 1.5 ml microtubes and blood collection tubes or 16 x 15 ml conical tubes, vertical, fits both H5000 and H5100    2719

H5250-4A                   Carousel for 4 x 250 ml Erlenmeyer flasks, fits both H5000 and H5100 2243

H5100-RODS             Carousel stacking rods, fits both  H5100 and H5000    306

 

H5500                Mini LabRoller Rotator with rotisserie for 36×1.5/2.0ml tubes, 120V    5234

H5500-230V              Mini LabRoller Rotator with rotisserie for 36×1.5/2.0ml tubes, 230V    5234

H5500-02                   Rotisserie for 36×1.5ml tubes (supplied with unit) 517

H5500-15                   Rotisserie for 10x15ml  conical tubes or (15-16 mm dia)and 12×12-13mm dia tubes  517

H5500-50                   Rotisserie for 6×50 ml tubes   517

 

H5600                Revolver Tube Mixer w/rotisserie for 36 x 1.5/2.0 ml tubes, 120V       5642

H5600-230V-EU                 Revolver Tube Mixer w/rotisserie for 36 x 1.5/2.0 ml tubes, 230V, EU  cord type        5642

H5600-230V-UK                 Revolver Tube Mixer w/rotisserie for 36 x 1.5/2.0 ml tubes, 230V, UK  cord type        5642

H5600-02                   Rotisserie assembly for 36 x 1.5/2.0  ml tubes     585

H5600-15                   Rotisserie assembly  for 10 x 15 ml tubes & 16 x 5/7 ml tubes    585

H5600-50                   Rotisserie assembly for 6 x 50 ml tubes 585

Shakers-3D

S0500                 GyroMini 3-D Shaker with dimpled rubber mat, 120V    5846

S0500-230V               GyroMini 3-D Shaker with dimpled rubber mat, 230V (add -EU or -UK to specify cord type) 5846

S0600                 Enduro MiniMix 3-D shaker with non-slip mat and two large blotting boxes, 120V       5370

S0600-230V               Enduro MiniMix 3-D shaker with non-slip mat and two large blotting boxes, 230V* (specify cord type by adding -EU or     -UK)      5370

E2110-BBL                 blot box, 9.1 x 6.6 cm, 3-5 ml capacity, for mini protein gels, 10/pk       721

E2110-BBS                 blot box, 11.7 x 8.9 cm, 6-10 ml capacity, for larger mini protein gels, 10/pk         640

 

S1000-B             GyroTwister 3-D Shaker with 30 x 30 cm non-slip platform and dimpled tube mat, 120V      14287

S1000-B-230V           GyroTwister 3-D Shaker with 30 x 30 cm non-slip platform and dimpled tube mat, 230V, Includes EU and UK power cords     14287

S1000-A-B                  GyroTwister Adjustable with 30 x 30 cm non-slip platform and dimpled tube mat, with adjustable tilt angle, 120V         16938

S1000-AB-230V                  GyroTwister Adjustable with 30 x 30 cm non-slip platform and dimpled tube mat, with adjustable tilt angle, 230V, Includes EU and UK power cords  16938

S1000-30           Stacking platform 30 x 30cm, with hardware 1767

S1000-40           Large platform, 40 x 40 cm, with non-slip rubber mat    2062

S1000-50           Extra large platform, 50 x 50 cm, with non-slip rubber mat   2357

S1000-M           Dimpled mat, 30 x 30 cm, for holding tubes   531

S2025-T             Elastic tie down, pk/4      162

Shakers-compact

S2025-B             ProBlot Rocker 25 with single platform (26 x 20 cm), 120V    10163

S2025-B-230V           ProBlot Rocker 25 with single platform (26 x 20 cm), 230V, includes EU and UK power cords       10163

S2025-D-B                  ProBlot Rocker 25D with double platform (26 x 20 cm), 120V         11341

S2025-D-B-230V                ProBlot Rocker 25D with double platform (26 x 20 cm), 230V, includes EU and UK power cords  11341

S2025-XL-D                ProBlot Rocker 25XL with large single platform (30 x 30 cm), 120V        11636

S2025-XL-B-30V                 ProBlot Rocker 25XL with large single platform (30 x 30 cm), 230V, includes EU and UK power cords 11636

S2025-XLD-B             ProBlot Rocker 25XLD with large double platform (30 x 30 cm), 120V   12741

S2025-XLD-B-230V            ProBlot Rocker 25XLD with large double platform (30 x 30 cm), 230V, includes EU and UK power cords         12741

S2025-T             Elastic tie down, pk/4      162

 

Plasticware – boxes

R7901                Storage box with hinged lid, blue, 50 x 1.5/2.0 ml (142 x 80x 46 mm) Available individually or case of 80     84

R7906                Storage box with hinged lid, blue, 50 x 0.5 ml (120 x 70x 37 mm) Available individually or case of 132 84

R7900                Storage box with telescoping lid, 50 x 1.5/2.0 ml (143 x 75 x 45 mm) Available individually or case of 96     83

R7905                Storage box with telescoping lid, 50 x 0.5 ml (122 x 62 x 33 mm) Available individually or case of 96   70

R7900-A            Numeric indexing grids for R7900 and R8600 boxes      83

R8300                Storage box with hinged lid, natural, 100 x 1.5/2.0 ml   96

R8300-BLK                 Storage box with hinged lid, opaque black for light sensitive samples, 100 x 1.5/2.0 ml Available individually or case of 36         111

R8300–X*                  Storage box with hinged lid, * green(-G), blue (-B), pink (-P), orange (-O) or yellow (-Y_ 100 x 1.5/2.0 ml  Available individually or case of 36  100

R8300-A            Rainbow 5 pack, includes one box of each color  6 pks of 5 per case of 30   464

R8315                Vertical rack for 9 boxes, fits chest type freezers   1856

R8316                Horizontal rack for 8 boxes, fits upright freezers   2165

 

Plasticware – tube racks

C7871                Microtube rack, clear, 40 x 0.5ml tubes (200 x 75 x 40 mm)  431

C7872                Microtube rack, clear, 40 x 1.5/2.0 ml tubes (210 x 75 x 35 mm)   408

C8210                Microtube support rack, red, 20 x 1.5ml/2.0 ml tubes (210 x 75 x 35mm)     175

Plasticware – storage, cooler, trays

R8000                Styrofoam storage box, 100 x 1.5/2.0 ml  Available individually or case of 100   61

D3500                “DyNA Chill portable cooler for  12 x 1.5ml tubes for 0°C

”       883

D3501                DyNA Chill portable cooler, for  12 x 1.5ml tubes, -15°C       865

C1205                Adapters for 0.5 ml tubes, pack of 6        568

凝胶成像系统

GDS-1302                   Enduro GDS Imaging System – Universal Voltage,Includes 5MP camera, prefocused lens, Orange filter, UV transilluminator, and Enduro GDS Image capture software.  302nm ,US,EU, UK plugs       92950

GDS-1365                   Enduro GDS Imaging System – Universal Voltage,Includes 5MP camera, prefocused lens, Orange filter, UV transilluminator, and Enduro GDS Image capture software.  365 nm US,EU, UK plugs       92950

GDS-12018                Clear Gel Tray,UV transmitting tray for gel handling      2645

GDS-12019                White Light Conversion Tray,UV to white light conversion screen for visible dyes          4790

GDS-12020                Blue Light Conversion Screen,Blue Light Conversion Screen For dyes requiring blue excitation    22000

GDS-12021                Printer, P-95DW,USB Connected Thermal Printer  26455

GDS-12022                Thermal Paper,High Density Paper, 4 rolls      2860

GDST-1302                Enduro GDS Touch Imaging System, 302 nm, includes 5 MP camera, manual F 1.4 lens, integrated Touchscreen computer, universal voltage    130000

GDST-1365                Enduro GDS Touch Imaging System, 365 nm,  includes 5 MP camera, manual F 1.4 lens, integrated Touchscreen computer, universal voltage, universal voltage 130000

GDST-497F                 Blue filter (497nm) for use with GDS touch only      16940

GDST-572G                Green filter (572 nm) for use with  GDS touch only       16940

GDST-676R                Red Filter (676 nm( for use with  GDS touch only 16940

GDS-TL1D-1               Total Lab 1D-1 user license,Gel Image Quantitative Analysis Software 7920

AccuTherm Microtube Shaking Incubator

I-4001-HCS                AccuTherm Microtube Shaking Incubator, heat/cool, 120V   37070

I-4002-HCS                AccuTherm Microtube Shaking Incubator, heat/cool, 230V   37070

I-4000-A            Block A, 40 x 1.5mL 2851

I-4000-B            Block B, 54 x 0.5mL 2851

I-4000-C            Block C, 96 x 0.2mL 2851

I-4000-D            Block D, 24 x 15mL  2851

I-4000-E            Block E, Water bath (ID: 115X73X38mm) (not suggested to use when mixing function is on)      3208

I-4000-H            Block H, 40 x 0.2mL 3208

I-4000-J             Block J, 96 Well ELISA Plate     4356

I-4000-G            Block G, 26 x 0.5mL and 24 x 1.5mL         4356

 

LIQUID HANDLING-瓶口分液器

D5370-2            Labmax 瓶口分液器, 0.3 to 2.5ml         4380

D5370-5            Labmax 瓶口分液器, 1.0 to 5.0ml         4380

D5370-10                   Labmax 瓶口分液器 2.0 to 10ml 4380

D5370-25                   Labmax 瓶口分液器, 5.0 to 25ml 6151

D5370-50                   Labmax 瓶口分液器, 10 to 50ml   6348

D5370-100                 Labmax 瓶口分液器, 20 to 100ml          9893

D5370-10HF              Labmax 瓶口分液器, 2.0 to 10ml         用于 氢氟酸 12316

D5370-25HF              Labmax 瓶口分液器 5.0 to 25ml          用于氢氟酸 16337

D5375-002                 延长放电管, 适合-2, -5 和 -10 瓶口分液器      254

D5375-012                 延长放电管, 适合 -25, -50 和-100 瓶口分液器         420

D5377                氯化钙干燥管,适合所有 Labmax 瓶口分液器   379

 

ENDURO™ VE20 VERTICAL GEL SYSTEM

 

E2020                NEW Enduro VE20   17277

E2020-CU                   Enduro VE20 Dual Casting System  17820

E2020-HITB              Enduro VE20 HS Blotting System, 20 x 20cm, High Intensity System including tank and lid, 1 casettes, 6 firbe pads, cooling coil      14630

E2020-IEF                   Enduro VE20 IEF Conversion Kit for 18cm IPG strips and Tube gels        1505

E2020-SDB                 Enduro Semi Dry Blotter 13992

E2020-SDBPS            Enduro Semi Dry Blotter/ 250V Power Supply Kit -115V 23747

E2020-SDBPS-230V           Enduro Semi Dry Blotter/ 250V Power Supply Kit -230V 23747

E2020-TBI                  Enduro VE20 Maxi Platinum Wire Blotting Inset, 4x compression cassettes and 18x fibre pads – standard          7106

E2120-C-.75-10                  Comb 10 sample, 0.75mm thick      408

E2120-C-.75-1-1                Comb 1 Prep, 1 Marker, 0.75mm thick   408

E2120-C-.75-18MC           Comb 18 sample MC, 0.75mm thick        408

E2120-C-.75-24                  Comb 24 sample, 0.75mm thick      408

E2120-C-.75-30                  Comb 30 sample, 0.75mm thick      408

E2120-C-.75-36MC           Comb 36 sample MC, 0.75mm thick        408

E2120-C-.75-48                  Comb 48 sample, 0.75mm thick      408

E2120-C-.75-5           Comb 5 sample, 0.75mm thick        408

E2120-C-1.5-10                  Comb 10 sample, 1.5mm thick        435

E2120-C-1.5-1-1                Comb 1 Prep, 1 Marker, 1.5mm thick      435

E2120-C-1.5-18MC           Comb 18 sample MC, 1.5mm thick 435

E2120-C-1.5-24                  Comb 24 sample, 1.5mm thick        435

E2120-C-1.5-30                  Comb 30 sample, 1.5mm thick        435

E2120-C-1.5-36MC           Comb 36 sample MC, 1.5mm thick 435

E2120-C-1.5-48                  Comb 48 sample, 1.5mm thick        435

E2120-C-1.5-5           Comb 5 sample, 1.5mm thick 435

E2120-C-1-10            Comb 10 sample, 1mm thick   421

E2120-C-1-1-1           Comb 1 Prep, 1 Marker, 1mm thick         421

E2120-C-1-18MC               Comb 18 sample MC, 1mm thick    421

E2120-C-1-24            Comb 24 sample, 1mm thick   421

E2120-C-1-30            Comb 30 sample, 1mm thick   421

E2120-C-1-36MC               Comb 36 sample MC, 1mm thick    421

E2120-C-1-48            Comb 48 sample, 1mm thick   421

E2120-C-1-5              Comb 5 sample, 1mm thick     421

E2120-C-2-10            Comb 10 sample, 2mm thick   449

E2120-C-2-1-1           Comb 1 Prep, 1 Marker, 2mm thick         449

E2120-C-2-18MC               Comb 18 sample MC, 2mm thick    449

E2120-C-2-24            Comb 24 sample, 2mm thick   449

E2120-C-2-30            Comb 30 sample, 2mm thick   449

E2120-C-2-36MC               Comb 36 sample MC, 2mm thick    449

E2120-C-2-48            Comb 48 sample, 2mm thick   449

E2120-C-2-5              Comb 5 sample, 2mm thick     449

E2120-CAST               Enduro VE20 Casting Base      2174

E2120-CC                   Enduro VE20 Detachable Cooling Coil     1672

E2120-DP                   Dummy Plate, 20 x 20cm         259

E2120-EC          Enduro VE20 External Casting Stand       2675

E2120-HITBI              Enduro VE20 HS Blotting System High intensity insert – includes 1 casette and 6 firbe pads         9472

E2120-LD          VS20  lid (No cables)      2718

E2120-MCB               Enduro VE20 Maxi Cooling Block.    669

E2120-NG-.75-BS              20 x 20cm Notched Glass Plates with 0.75mm Bonded Spacers (Pack of 2)   1198

E2120-NG-1-BS                  20 x 20cm Notched Glass Plates with 1mm Bonded Spacers ( Pack of 2)        1198

E2120-NG-2               20 x 20cm Notched Glass Plates 2mm thick ( Pack of 2) 853

E2120-PG-.75-BS               20 x 20cm Plain Glass Plates with 0.75mm Bonded Spacers (Pack of 2)          737

E2120-PG-1.5-BS               20 x 20cm Plain Glass Plates with 1.5mm Bonded Spacers (Pack of 2)   737

E2120-PG-1-BS                   20 x 20cm Plain Glass Plates with 1mm Bonded Spacers ( Pack of 2)     737

E2120-PG-2               20 x 20cm Plain Glass Plates 2mm thick ( Pack of 2)        414

E2120-PG-2-BS                   20 x 20cm Plain Glass Plates with 2mm Bonded Spacers (Pack of 2)      737

E2120-PI           Enduro VE20 Page insert          5295

E2120-PW                  Replacement Platinum Wire 0.2mm – 100cm 3277

E2120-RM                  Enduro VE20 Replacement Rubber mats for 20 x 20cm caster.      585

E2120-S-.75               20cm Spacers – 0.75mm (Pack of 2)         259

E2120-S-1                   20cm Spacers – 1mm thick (Pack of 2)     285

E2120-S-1.5               20cm Spacers – 1.5mm thick (Pack of 2)  298

E2120-S-2                   20cm Spacers – 2mm thick (Pack of 2)     310

E2120-T             VS20 Tank        6524

E2120-TBC                 Enduro VE20 Blot Maxi Cassette    777

E2120-TBFP               Fibre pads – Pack of 6       777

 

Labnet Optipette 系列移液器

 

5691                   Optipette 0.1~2.0ul系列移液器    880

5692                   Optipette0.5~10ul系列移液器      880

5693                   Optipette2~20ul系列移液器 880

5694                   Optipette10~100ul系列移液器     880

5695                   Optipette20~200ul系列移液器     880

5696                   Optipette100~1000ul系列移液器 880

5698                   Optipette1000~5000ul系列移液器        880

5699                   Optipette1000~10000ul系列移液器     880

U1001                         19612

U1002                         20790

E0304                          5600

GDBL-1000                         120000

 

聚甲基丙烯酸钠标准品 Polymethacrylic Acid-Na Salt

发表于由

上海金畔生物科技有限公司提供聚甲基丙烯酸钠标准品 Polymethacrylic Acid-Na Salt

聚甲基丙烯酸钠标准品 Polymethacrylic Acid-Na Salt
水性凝胶色谱标准品（Aqueous GPC/SEC Standards）
（1）窄分子量分布聚甲基丙烯酸钠标准品套装
Narrow MWD Polymethacrylic Acid – Na Salt
Catalog # Mp Mw Mn
PMAAKIT The standards listed below are sold only as a kit
每种200mg 1,027,000 947,000 643,000
549,000 483,000 429,000
210,000 192,000 186,000
78,300 75,100 73,300
31,500 31,100 30,400
15,400 15,200 14,100
6,610 6,510 5,560
3,570 3,870 3,290
1,670 1,700 1,520
1,220 1,250 1,040
The standards listed above are sold only as a kit
（2）窄分子量分布聚甲基丙烯酸钠标准品
Narrow MWD Polymethacylic Acid – Na Salt
Catalog # Mp Mw Mn
PMAA720K 720,000 685,000 620,000
PMMA171K 171,000 150,000 125,000
PMAA86K 86,000 84,000 81,000
PMAA8K 8,200 8,400 7,600

更多产品，更多优惠，请联系我们！
上海金畔生物科技有限公司
地 址： 上海市浦东新区东靖路699弄36栋701室
邮 编： 201208
固话总机：
订货热线：15221999938
网 址： www.jinpanbio.com
金畔博客：www.jinpanbio.cn
Email：sales@jinpanbio.com

愈创木酚检测试剂盒

发表于由

产品编号 产品名称 产品规格 产品等级 产品价格
KYO-06810 YSG Agar Plate
YSG琼脂平板		 20 PLATE – –
KYO-08900 Va-YSG Medium
脂环酸芽孢杆菌/嗜热耐酸芽孢杆菌 培养基		 2mL×100
KYO-08920 Guaiacol Detection Kit
愈创木酚检测试剂盒		 100TEST

愈创木酚检测试剂盒愈创木酚检测试剂盒

Guaiacol Detection Kit



◆背景

 愈创木酚检测试剂盒

嗜酸耐热菌Alicyclobacillus acidoterrestris是革兰氏阳性，产芽胞厌氧菌，在自然界中普遍存在。1984年透明苹果汁混浊事件后该物种被大量研究。生长在相对高的温度和酸性条件下的Alicyclobacillus类细菌，称为热-嗜酸性杆菌（TAB） [1, 2, 3].

TAB不能通过巴氏消毒法被灭菌，可能残留在合成的食物和饮料中。尚不清楚TAB是否对人体有害，但产生有异味的愈创木酚，会降解果汁和其他产品的质量。即使没有异味，在分配过程中轻微恶化引起的降解也会降低产品的质量。                                                                                                                               Va-YSG Medium  

避免TAB污染是非常困难的，特别是在土壤中。幸运的是，引起污染的   

A. acidocaldarius 和其他主要物种被认为是无害的，TAB引起的损害能够通愈创木酚检测试剂盒

过监测被认为有害的A. acidoterrestri来缩小。因为这些物种生长相对高温

和酸性的条件下，不能通过常规的提取培养的方式进行检测。                 

由于欧洲和其他区域经常发生果汁质变事件，2003年3月日本果汁联合会研发和出版了同一检测TAB方法 [4]。为了容易进行该项检测，我们研发了该试剂盒。该试剂盒不同于会产生异味的愈创木酚的A. acidoterrestri试剂盒。Guaiacol Detection Kit Reference                                                                                        Guaiacol Detection Kit                                                                                                         

                                                                                                                                                                                       

           

 

◆试剂盒组成


YSG 琼脂板20 块板(货号KYO-06810)

TAB纯化的YSG琼脂培养基

10×2 板/1 盒

 

Va-YSG 培养基 (2mL) 100 个管子(货号KYO-08901)

加入到YSG培养基的Vanillic acid ，用于愈创木酚的形成

2 mL×100 管

 

愈创木酚检测试剂盒 100 次 (货号KYO-08921)

试剂 1: 50mM 苯二甲酸氢钾缓冲液

60 mL×2 瓶

试剂 2: 1.3 % 过氧化氢溶液

2.5 mL×1 管

试剂 3:过氧化物酶-磷酸缓冲液

2.5 mL×1 管

阳性对照: Guaiacol  (1050 ppm)

2.5 mL×1 管

 


◆TAB测试流程图-日本果汁协会

愈创木酚检测试剂盒

  

◆实验流程


1. 用接种环，10uL，在YSG琼脂板上提取的新克隆，该克隆还没有形成孢子。在“Va-YSG medium (2mL), ” (≥1×108cfu/mL)管内重悬该克隆。.

2. 孵育 45±1°C，3小时。

3. 加入1 mL“Guaiacol Detection Kit”的试剂1。

4. 加入20 μL“Guaiacol Detection Kit”的试剂 2 。

5. 加入20 μL 试剂3 of “Guaiacol Detection Kit”试剂3.

6. 混匀。

7. 放置5-10 分钟。

8. 检测培养基颜色的变化。*请在10分钟内观察颜色变化。


愈创木酚检测试剂盒


愈创木酚检测试剂盒

Brown color indicates contamination of AAT

愈创木酚检测试剂盒


空白 (阴性对照) 

使用没有接种的"Va-YSG medium (2mL)" ，进行步骤(2)-(8).


阳性对照

2 mL灭菌水和100 µL阳性对照混合，进行步骤 (3)-(8).

处理信息

• 请使用高压灭菌方式处理已经使用的培养基（121℃，超过30分钟)

• 保存条件: 2-10 ℃，避光。


• 保质期：制造后12个月

一般我们提供的产品保质期在3个月到10个月。

欲了解详情资料请点击：果汁中嗜热嗜酸菌的检测方法——膜过滤法和涂布法

愈创木酚检测试剂盒

Guaiacol Detection Kit Flyer
PDF Download [419 kb] 



Q1：  我对TAB列举法有些问题。

          a. YSG灭菌水是什么意思，我想知道制备的比率。

          b.阳性/阴性测试：样品制备步骤提到的用YSG培养基稀释，稀释比例是多少？
          c.如果是水样品的话，是不是用相同方法？

A1：   (a,b)回答: 
           "YSG distilled water"是错误说法，正确的说法是“灭菌水”。制备比例的话，请参考下面的

           信息，不过具体案例具体分析。(参考数据来自日本果汁联合会R).
           ——————————————————–
           果汁　　　　　　〔Brix 值〕　 　参考比例　　
           ———————————————————
           苹果　　　　　　　 　〔70〕　････････ 10 倍
           橙子　　　　　　　〔65〕　････････ 10 倍
           红葡萄　　　　　〔65〕　････････ 20-50 倍
           白葡萄　　　 〔65〕　････････ 20 倍
           胡萝卜 　　　　　　 　〔40〕　･････････10 倍
           柠檬　　　　 　〔50〕　･････････10 倍　　　
           葡萄柚　　 　〔55〕　･････････20 倍
           桃子　　　　　　　 〔55〕　･････････10 倍
           ———————————————————
            c. 水样品的话，是不是用相同方法？
            回答(c):对不起，我们不能明确告知是否可以运用，我们手头没有相关记录。
           有一种软饮料“near water"，来自调味品，不是果汁（或者少量）和水。我们听说该款产品可用

           于检测该类饮料。 

Q2：   果糖能够作为样品吗？

A2：   非常抱歉，我们这边没有关于愈创木酚检测糖果（果糖）样品的相关知识。所以我们建议参考简单

           流程，该流程修改自 “Japanese spore counting method of sugar samples”。如果你们有预处

           理微生物测试的流程，请按照你们的操作流程操作代替操作操作流程中的“样品制备“。该流程中

           有制备YSG液体培养基的方法。如果 您使用我们提供的流程，不需要使用接种环和YSG琼脂板。

Q3：   我们使用该试剂盒鉴定Alicyclobacillus acidoterrestris。我们的客户是做墨西哥沙拉的，它们也

           有类似愈创木酚的异味。我们是否可以用该试剂盒去检测沙拉中的愈创木酚(主要西红柿，红色)？红

           色是否会干扰？

A3：   我们这边没有数据是关于愈创木酚检测试剂盒检测沙拉酱的案例。如果您考虑观察方法，请参考下

           面的"注意事项" 。

           注意事项（1）：来自样品的固体如果样品的固体中有AAT，必须采取胃部处理或者匀浆去除固体的致

           病菌。

           注意事项（2）：材料中多酚的影响多酚是AAT（Alicyclobacillus acidoterrestris）生长抑制剂。因此，

           检测AAT必须稀释样品20~50倍。否则，无法检测出来。我们不清楚沙拉中有多少多酚。但是西红

           柿是含有多酚的。一般稀释10倍。可以考虑沙拉稀释超过20倍。

           注意事项（3）：关于样品 的粘度。稀释降低粘度。因子样品必须适当稀释。请参考微生物稀释比例检测

           样品，不包括抗热嗜酸性细菌。注意事项(4):关于样品到标本上携带的颜色如果最终采用过氧化物酶

           方法，如果样品带有棕色的话，可能判断起来很麻烦。如果样品稀释的话，我认为不会引起大问题。

           注意事项（4）：其他干扰材料如果使用过氧化物酶方法，通过过氧化物酶反应会使愈创木酚 (AAT产物，

           会引起异味)聚合并且变成棕色。我们认为沙拉酱包括不同的香味料可以作为材料。因此，如果有材料

           抑制过氧化物酶反应或者含有的物质可以起过氧化酶作用，通过过氧化物酶反应变成棕色，会引起假

           阳性或者假阴性的结果。

Q4：  如何接种TAB到YSG琼脂板上面？请提供流程。

A4：    根据Japan Fruit Juice Association，有3种方法

            1) 倾注平皿的方法

            2) 用曲面玻璃棒涂布平板的方法 
            3) MF 方法
            如果告知客户的样品和检测方法，我们可以推荐方法。

            对于您的信息，请让我们知道在我们手册里面TAB检测的流程。


Q5：    是否提供不含香草酸的YSG培养基？

A5：    是的，我们提供不含香草酸的YSG培养基。然而售价会高，也需要额外时间考虑生产。另外，小批量生产

            会比较难。

Q6：    是否有数据或者参考文献显示 Guaiacol detection kit的准确性? 我们的客户希望提供上述信息。

A6：    关于产品的准确性，Japan Institute of fruit juice认可检测操作手册还有手册上提到的批次准确性的数据。

            然后，英文版的操作搜出不提供准确性的信息。

            关于您的要求，请参考下面有研发者by developer(Motohiro Niwa)写的论文 "Development of a rapid 

            detection method of A. acidoterrestiris, Hazardous bacteria to acidic beverage"


Q7：   我们需要下面两者之一的微生物菌株A.acidobacillus 或者 A.acidoterrestris 去完成分析，制定质量政

            策。是否可以从你们那边获得？

A7：   非常抱歉我们不能提供细菌菌株。您可以通过ATCC和DSMZ的当地经销商联系购买。

           http://www.atcc.org/About/tabid/138/Default.aspx
           http://www.dsmz.de/
           下面的菌株是分泌愈创木酚的。
           ———————————————————-
          ・　A.acidoterrestris　ATCC49025
          ・　A.acidoterrestris　DSM2498
          ・　A.acidoterrestris　DSM3923
          ・　A.acidoterrestris　DSM3924

Q8：    该试剂盒是否可用于牛奶或者啤酒？

A8：     关于牛奶和啤酒，我们没有相关的信息。


Q9：    该试剂盒可以检测多少愈创木酚？

A9：     Va-YSG 培养基 和Guaiacol detection kit能够检测25ppm愈创木酚。


Q10：  是否与关于假阳性结果的信息？

A10：   如果样品中有过氧化物酶或则过氧化氢酶的话，我们试剂盒可能还有假阳性的结果。


Q11：  是否可用BAT培养基代替YSG琼脂？

A11：   他们的YSG琼脂用这个试剂盒是可以的。我们推荐使用Va-YSG (KYO- 08901)用于下一步实验室（在YSG琼

             脂上孵育后）。我们的试剂盒是基于 Japan Fruit Juice Association的操作流程， 不推荐使用BAT培养

              基。所以不能保证使用BAT培养基的结果。


Q12：  是否可用来检测糖果？

A12：   对不起我们这边没有这方面信。可以参考 “Japanese spore counting method of sugar samples”。

             如果您与细菌检测的预处理流程，请使用您的实验流程代替样品处理。该流程中，有YSG液体培养基

             的制备方法。如果 您使用我们建议的流程，需要接种环和YSG琼脂板。如果不适合您的样品，建议修改。


Q13:    该试剂盒是否可以检测Ethylvanillin 到Ethoxyphenol转变?Ethoxyphenol也会类似愈创木酚，会有

            异味？

A13：  该试剂盒有比色法的结果，通过过氧化物酶反应愈创木酚转变成四-愈创木酚检测愈创木酚。关于2-

            Ethoxyphenol, 也会发生过氧化物酶反应，会有比色法结果。然而我们之前没有测试过，也没有看

           到相关的文献，不能确定。在很多化学物质数据库中我们也没有找到关于异味是由Ethoxyphenol引

           起的信息。


Q14：  该试剂盒可检测蜂蜜吗？

A14:     对不起，我们没有做过类似实验。

愈创木酚检测试剂盒

Guaiakol_detection_kit_reference.pdf



【1】   G.Deinhard, et al., Bacillus acidoterrestris sp. nov., a new thermotolerant acidophile isolated from

            different soils. Syst. Appl. Microbiol. 10, 47-53 (1987)

【2】  GOTO, M., Spore-forming thermo-acidophilic bacilli of the genus Alicyclobacillus, Bokin Bobai, 28

           (8), 499-508 (2000), in Japanese

【3】  GOTO, K., Lecture on "Classification and identification of microbes" 1. Classification and properties

           of new thermo-acidophilic bacilli, January 2001 issue of Soft Drink Technical Data, 9-26 (2001), in 

           Japanese

【4】  Niwa, M., et al., Development of a rapid detection method of A.acidoterrestris, hazardous bacteria

           to acidic beverage. Fruit processing, 13, 102-107 (2003)

【5】  Japan Fruit Juice Association, On the unified test method for thermo-acidophilic bacilli, Report of 

           Japan Fruit Juice Association, 535, 4-12 (2003), in Japanese

【6】  Niwa, M., et al., A.acidoterretris rapid detection kit, Fruit processing, 13, 328-331 (2003)

【7】  Niwa, M., Control of deleterious bacteria in acidic beverages by using a guaiacol detection kit

           (peroxidase method), Japan Food Science, 2004-2, 23-28 (2004), in Japanese

【8】  Japan Laid-Open P2003-259A “Detection of guaiacol forming bacteria and/or its identification 

           method”

i-BOX（自动型/非自动型）

发表于由

产品编号 产品名称 产品规格 产品等级 产品价格
WEB0151 Forest（自动型） 253mm×313mm×358mm（35L） – –
WEB0152 Tropical（自动型） 253mm×313mm×358mm（35L） – –
WEB0153 Ocean（自动型） 253mm×313mm×358mm（35L） – –
WEB0154 Snow（自动型） 253mm×313mm×358mm（35L） – –
WEB0155 Moon（自动型） 253mm×313mm×358mm（35L） – –
WEB0316 Forest（非自动型） 253mm×313mm×358mm（35L） – –
WEB0317 Tropical（非自动型） 253mm×313mm×358mm（35L） – –
WEB0318 Ocean（非自动型） 253mm×313mm×358mm（35L） – –
WEB0319 Snow（非自动型） 253mm×313mm×358mm（35L） – –
WEB0320 Moon（非自动型） 253mm×313mm×358mm（35L） – –

i-BOX

 

i-BOX（自动型/非自动型）

◆原理


自动型干燥箱为可设定保持温度恒定，将湿的玻璃器皿烘干的大体积的容器。实际的温度可由附于其中的温度计直接读出。因为水的沸点为摄氏一百度，故一百度的温度必可使玻璃器皿很快烘干。但是在八十度以上时，水的蒸气压即相当大，足可令湿的器皿很快的烘干。而且，即使在做定量分析的实验中，所有烘干的器皿也都要在室温的情形下使用，故温度太高的器皿要恢复到室温所需的时间也会拉长；因此干燥箱的使用温度不必一定要设定在水的沸点之上。干燥箱也可用来干燥 化学药品之用，此时若是要排除吸附在化合物表面上的小分子，则干燥箱的温度就必须设定在摄氏一百二十度以上加热两小时，才足以使水分子驱走，如此才可测得该物的真正重量。

 

◆优点・特色

 

手动型 自动型

●　附有硅胶的非自动经济型干燥箱。

●　可以作为收纳箱使用。

●　多款颜色可以选择。

●　搭载全自动除湿器(DU-2)。

●　柜内湿度保持在30~40%RH之间。

●　能够叠放使用， 十分便利。

 

◆案例・应用

● 干燥箱广泛运用于液体抽气，试剂、感应器、光学制品、量表、精密仪器的保管，也用于包装实验中。适用于做减压真空实验
● 超小型丙烯酸真空干燥器方便携带（200g~620g）
● 适用于实验室微量样品、土壤样品的保管、也可用于室外取样
● 本体和盖子都是完全透明的聚碳酸酯干燥器，便于查看，不会轻易打烂或刮花
● 箱盖接口为O型的密封圈，无须涂凡士林。料耐热性强、拉伸性、冲击性强。


i-BOX（自动型/非自动型）

三博特干燥箱单页

【自动型】

Q：    目前正使用玻璃制的干燥箱。这个产品暂时作为除湿保管箱使用，请问除湿效果能达到什么样的程度呢？
A：    i-box是有着能把将实验室的空间最大程度地有效利用的外形和干燥箱的性能这两者很好地平衡的一款产品。

          通过背部搭载的除湿接口DU-2，箱内能全自动地不断保持30～40%RH的除湿效果。
          无需任何繁琐的硅胶更换。
          可以把该产品作为重要资料的保管箱，请安心使用。

Q：    i-box是什么材质？
A：    本体是ABS，门是AS。


【手动型】


Q：    这个商品是自动型的干燥箱吗？
A：    不是的。这个产品不搭载除湿自动接口。需在箱内放置硅胶（干燥剂），并定期更换。
         「i-box」也有自动系列，如有兴趣可于本网站搜索查阅。

Q：    能把聚合物粉末放进里面保管吗？
A：    聚合物粉末是能引起粉尘爆炸的物质。本产品靠近直射性阳光、火、电力类（含静电）等将非常危险，请谨

          慎放置需保管物品。

海藻糖检测试剂盒Trehalose Assay Kit Trehalose Assay Kit 货号：K-TREH Megazyme中文站

发表于由

海藻糖检测试剂盒Trehalose Assay Kit

英文名：Trehalose Assay Kit

货号：K-TREH

规格：100 assays (manual) / 1000 assays (microplate)

The Trehalose test kit is a simple method for the rapid and reliable measurement and analysis of trehalose in foods, beverages and other materials.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Trehalose and
D-Glucose in foodstuffs, beverages, and other materials

Principle:
(trehalase)
(1) Trehalose + H2O → D-glucose

(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size:                            100 assays (manual) / 1000 (microplate) 
                                          / 1100 (auto-analyser)
Method:                             Spectrophotometric at 340 nm
Reaction time:                   ~ 8 min
Detection limit:                  37.5 mg/L
Application examples: 
Honey, mushrooms, bread, beer, seafood (e.g. lobster and shrimp),
fruit juices, purees and fillings, nutrition bars, surimi, dehydrated fruits
and vegetables, fruit products, white chocolate, sports drinks, dairy
products, egg products, soups and sauces, confectionery, chewing gum,
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:           Novel method

Advantages

  • Only enzymatic kit available
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

 

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q5. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q6. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q8. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q9. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q10. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q11. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q12. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q14. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.