Sigma 二甲基亚砜DMSO

Sigma 二甲基亚砜DMSO

产品编号 D2650 | CAS号 67-68-5 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

DMSO 是一种用于化学反应(如聚合酶链式反应 (PCR))的极性非质子溶剂,也可作为冷冻保护玻璃化剂用于保存细胞、组织和器官。DMSO 可在细胞冷冻培养基中用于保护细胞免受冰晶导致的机械损伤。它可用于原代、继代培养和重组的异倍体和杂交瘤细胞系、胚胎干细胞 (ESC) 以及造血干细胞的冻存。DMSO 常与 BSA 或胎牛血清 (FBS) 一同使用。

Caution

容易过度冷却,并在室温下缓慢融化固化的产品放在室温下可以再次液化,对其本身无影响。

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

包装:5mL 和 10mL 火焰封口安瓿瓶包装,100mL 棕色瓶包装

sigma 醋氨酚BioXtra

sigma 醋氨酚BioXtra

产品编号 A7085 | CAS号 103-90-2 | SIGMA-ALDRICH

Application:镇痛剂。

包装:1 kg in glass bottle,100, 500 g in glass bottle

Related Categories Building Blocks, C6 to C8, Chemical Synthesis, Organic Building Blocks, Oxygen Compounds,

更多…
product line BioXtra
assay ≥99.0%
form powder
impurities ≤0.0005% Phosphorus (P)
≤0.1% Insoluble matter
ign. residue ≤0.1%
mp 168-172 °C(lit.)

Sigma 孕酮Progesterone

Sigma 孕酮Progesterone

产品编号 P8783 | CAS号 57-83-0 | SIGMA

英文名:Progesterone
中文名:1.孕甾酮 2.黄体酮 3.黄体素 4.孕酮  5.孕醇 6.黄体激素
Synonym: 4-Pregnene-3,20-dione
CAS Number 57-83-0
Empirical Formula (Hill Notation) C21H30O2
Molecular Weight 314.46
description:synthesized from materials derived from plant source
product line:BioReagent
form:powder
potency:0.1-20 ng/mL
mp:128-132 °C(lit.)
solubility :absolute ethanol: soluble1 mg/mL
suitability :uitable for cell culture

Application

用于细胞培养,研究细胞信号传导和基因表达的甾醇调控

Other Notes

由卵巢黄体产生的类固醇激素。

包装:1, 5, 25 g in glass bottle

Reconstitution

To prepare 20μg/ml stock solution, add 1ml absolute ethanol per mg progesterone, gently swirl to dissolve, add 49ml sterile medium per ml ethanol.

Physical form

室温下为粉末状;分成小份冻存,以避免使用时反复冷冻/解冻

Biochem/physiol Actions

诱导子宫内皮细胞的成熟和分泌活动;抑制排卵。孕酮与乳腺癌的病因有关。

sigma-白蛋白 来源于牛血清

sigma-白蛋白 来源于牛血清

产品编号 A3803 | CAS号 9048-46-8 | SIGMA

包装:10, 50, 100 g in poly bottle

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

采用热休克分级分离法制备。经过进一步纯化以降低脂肪酸含量。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Related Categories Albumin, Bovine Serum Albumins, Heat Shock Fractionated, 生化试剂, 蛋白质及衍生物 更多…
assay ≥98% (agarose gel electrophoresis)
form lyophilized powder
mol wt mol wt ~66 kDa
purified by heat shock fractionation
packaging poly bottle of
origin USA origin
impurities ≤0.005% Fatty acids

 

产品编号 A8806 | CAS号 9048-46-8 | SIGMA

包装:1, 5 g in poly bottle

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

通常称为 Cohn fraction V;本品采用改进的 Cohn 氏冷乙醇分级分离法制备。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.[]

Protocols & Applications

Select the right albumin for your application with the Albumin Selection Guide

Related Categories Albumin, Albumins, Albumins and Transport Proteins,Albumins by Application, Bioactives and Supplements for Stem Cell Expansion,

更多…
Quality Level PREMIUM
product line BioReagent
assay ≥96% (agarose gel electrophoresis)
form lyophilized powder
mol wt mol wt ~66 kDa
purified by cold ethanol fractionation
packaging poly bottle of

Sigma人血管紧张素 II

Sigma人血管紧张素 II

产品编号 A9525 | CAS号 4474-91-3 | SIGMA

Angiotensin II human    ≥93% (HPLC), powder

CAS号 4474-91-3
分子式: C50H71N13O12
分子量 1046.18
assay    ≥93% (HPLC)
form    powder
total impurities    1.2-10.4% acetate
storage temp.  -20°C
Amino Acid Sequence
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
Antibody Adsorbant
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-OH · 2CH3COOH · H2O
Analysis Note
肽含量不低于 75%
Biochem/physiol Actions
血管紧张素 (Ang) II 在调节心血管的血液动力学和结构中起重要作用。Ang II 在成人组织内的大部分已知效应均可归因于血管紧张素 II 类型 I (AT1) 受体。AT1 和 AT2 受体在药理学和生物化学性质上有差别,在心血管血液动力学和细胞生长方面表现出相反的效应。此外,肾素-血管紧张素和一氧化氮生成系统在心血管功能的调节中显示出相互作用的现象。Ang II 刺激血管生成,增加微血管密度。事实上,血管紧张素 II 是由血管紧张素转换酶对血管紧张素原的作用而生成;C-端-组氨酰-亮氨酸裂解。
保存条件: -20°C

Sigma 阿霉素 盐酸盐

Sigma 阿霉素 盐酸盐

产品编号 D1515 | CAS号 25316-40-9 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

包装:10 mg in glass bottle Biochem/physiol Actions

Naturally fluorescent anthracycline antibiotic, anticancer drug. Doxorubicin is a substrate of MRP1 which was first cloned from a DOX-resistant lung cancer cell line. Fluorescent property has been exploited for the measurement of drug efflux pump activities as well as resolving the important question of intracellular localization of various multidrug resistance proteins and the role of subcellular organelles (Golgi and lysosome) in the sequestration of drugs and its implication in drug resistant phenotypes.

法律信息

Adriamycin 为以下机构的注册商标: RDF Pharmacia & Upjohn S.P.A.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antibiotics by Application, Antineoplastic and Immunosuppressive Antibiotics,

更多…
assay 98.0-102.0% (HPLC)
mp 216 °C (dec.)(lit.)
solubility H2O: soluble50 mg/mL, clear
Mode of action DNA synthesis | interferes
antibiotic activity spectrum viruses
storage temp. 2-8°C

Sigma 放线菌素 D

Sigma 放线菌素 D

产品编号 A9415 | CAS号 50-76-0 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas. Recommended for us in cell culture applications at 1μg/mL.

Caution

This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

包装:2, 5, 10, 25 mg in glass bottle

Preparation Note

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Biochem/physiol Actions

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma丝裂霉素 C 来源于头状链霉菌

Sigma丝裂霉素 C 来源于头状链霉菌

产品编号 M0503-2mg | CAS号 50-07-7 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.

Biochem/physiol Actions

DNA 合成、细胞核分裂和癌细胞的抑制剂。对革兰氏阳性菌、革兰氏阴性菌、抗酸杆菌有抗菌性。

Caution

This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared – the precipitated solution has been proven toxic to cells.

Preparation Note

Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma特殊产品Thiopropyl–Sepharose® 6B

Sigma特殊产品Thiopropyl–Sepharose® 6B

产品编号 T8387 | CAS号 68517-67-9 | SIGMA

包装:1, 5, 15 g in poly bottle

Physical form

Lyophilized powder stabilized with lactose and dextran

Application

Thiopropyl Sepharose® 6B is used in protein chromatography, affinity chromatography and hydrophobic interactions. Thiopropyl Sepharose® 6B has been used for the removal of inhibitors from PCR extracts from evidence collected for law enforcement murder investigations. Thiopropyl Sepharose® 6B has also been used to describe a method for the investigation of functional properties of distinct domains of viral thiol proteins, including the influenza virus membrane M1 protein.

法律信息

Sepharose is a registered trademark of GE Healthcare