ad-MED Vitrigel ™ 2

ad-MED Vitrigel™ 2 is a cell culture insert using a collagen Vitrigel™ membrane as a scaffold for cell culturing. ad-MED Vitrigel™ 2 indicates high adhesive property against various kind of animal cells. Also, it is useful for fabrication of three- dimensional tissue models appropriate for drugs and cosmetics development. Recently, it’s considered as a material for MPS devices as well, and it’s a product with high potential.

Vitrigel™ is a registered trademark of National Agriculture and Food Research Organization (NARO).
This product is supported by Agri-Health Translational Research Project from the Ministry of Agriculture,Foresty and Fisheries of Japan.

Collagen Vitrigel™

When the moisture of the collagen gel is removed, the collagen fiber density increases and the collagen gel turns into a film. Because this dehydration process is called “Vitrification”, the film produced by this method is called “Vitrigel™ ”.

Features

Excellent visibility for observation
These images indicate transparency of each membranes. Each cell culture inserts were placed on the black cloth after rehydration with PBS. Transparency of collagen Vitrigel™ membrane is higher than that of PET or PTFE membrane. Collagen Vitrigel™ membrane is low fluorescent membrane. It makes high-contrast fluorescent observation.

High adhesive property / Promotion of cell spreading
ad-MED Vitrigel™ 2 indicate excellent cell adhesive property and proliferation acitivity as compare to other cell culture inserts using plastic membrane.

Permeability
FITC-dextran (MW:4,000 or 10,000 or 40,000) was used as a permeable substance.These results suggested that the permeability of collagen vitrigel membrane was higher than that of PET (0.4 μm) membrane and dependent on the molecular weight of a substance unlike the PET membrane.

Long-term culture
The Vitrigel™ culture method can maintain CYP metabolic activities for at least 3 weeks.

*this data is cited by ChemTime 266 B

Designs for easier handling

Appropriate for constructing co-culture systems
ad-MED Vitrigel™ 2 allow to co-culture different type of cells on both inner and outer side of collagen Vitrigel™ membrane by using option ring.

Collagen Vitrigel™ 2 membrane
We also supply Vitrigel™ in processable membrane form in 12 well or 24 well size.
Custom-made products are also available, please contact us.

Product lineup

brochure

ad-MED Vitrigel™ 2 series

References

Takezawa T. et al. J Toxicol Sci. 47: 39-50, 2022.
Morimoto Y. et al. Sensors and Actuators B: Chemical. 38: 130675, 2021.
Yoshida S. et al. Stem Cell Reports. 16: 295-308, 2021.
Uzu M. et al. J Pharmacol Toxicol Method. 106: 106916, 2020.
Shima A. et al. In Vitro Cell. Dev. Biol. Anim. 56: 500-504, 2020.
Ogaki A. et al. Neuropsychopharmacol Reports. 40: 307-313, 2020.
Moriyama M. et al. Scientific Reports. 9: 18371, 2019.
Nakai S. et al. Biol Open. 8 (7): bio042192., 2019.
Watari R. et al. Biol. Pharm. Bull. 42: 348-353, 2019.
Yamaguchi H. et al. Drug Metab Dispos. 46: 1684-1691, 2018.
Watari R. et al. J Toxicol Sci. 43: 521-529, 2018.
Yamaguchi H. et al. Journal of Applied Toxicology. 36: 1025-1037, 2016.
Yamaguchi H. et al. Toxicol Sci. 135: 347-355, 2013.
Takezawa T. Journal of Biosciences and Bioengineering. 91: 214-217, 2013
Takezawa T. et al. Toxicol In Vitro. 25: 1237-1241, 2011.
Takezawa T. et al. Cell Transplant. 13: 463-473, 2004.

 

ad-MED vitri gel 2

ad-MED Vitrigel 2是一种细胞培养插入物，使用胶原vit rigel膜作为细胞培养的支架。ad-MED vit rigel 2显示出对各种动物细胞的高粘附性。此外，它还可用于制造适合药物和化妆品开发的三维组织模型。最近，它也被认为是MPS设备的一种材料，是一种极具潜力的产品。

Vitrigel是NARO国家农业和食品研究组织的注册商标。
该产品得到日本农林水产省农业健康转化研究项目的支持。

胶原蛋白透明质酸

当胶原凝胶的水分被去除时，胶原纤维密度增加，胶原凝胶变成薄膜。因为这种脱水过程被称为“玻璃化”，所以用这种方法生产的薄膜被称为“Vitrigel”。

特征

极佳的观察视野
这些图像显示了每个膜的透明度。用PBS复水后，将每个细胞培养插入物放在黑布上。胶原Vitrigel膜的透明度高于PET或PTFE膜。胶原Vitrigel膜是低荧光膜。它可以进行高对比度荧光观察。

高粘附性/促进细胞扩散
与使用塑料薄膜的其他细胞培养插入物相比，ad-MED vit rigel 2显示出优异的细胞粘附性和增殖活性。

弥漫
FITC葡聚糖（分子量:4000或10000或40000）被用作渗透性物质。这些结果表明胶原玻璃体膜的渗透性高于PET（0.4微米）膜的渗透性，并且依赖于不同于PET膜的物质的分子量。

长期文化
Vitrigel培养方法可以保持CYP代谢活性至少3周。

*该数据由ChemTime 266 B引用

易于操作的设计

适合构建共培养系统
ad-MED Vitrigel 2允许通过使用选装环在胶原vit rigel膜的内侧和外侧共培养不同类型的细胞。

胶原vit rigel 2膜
我们还提供12孔或24孔尺寸的可处理膜形式的Vitrigel。
也可定制产品，请联系我们。

产品阵容

小册子

ad-MED vitri gel 2系列

参考

Takezawa T .等《毒物科学杂志》。47: 39-50, 2022.
传感器和执行器B:化学。38: 130675, 2021.
吉田等人的干细胞报告。16: 295-308, 2021.
药物毒理学方法。106: 106916, 2020.
Shima A .等人的体外细胞。戴夫。生物。动漫。56: 500-504, 2020.
Ogaki A .等人的神经精神药理学报告。40: 307-313, 2020.
森山m .等人的科学报告。9: 18371, 2019.
Nakai S .等人生物开放。8（7）:生物042192。, 2019.
Watari R .等人。制药。公牛。42: 348-353, 2019.
山口百惠等药物代谢物处理。46: 1684-1691, 2018.
Watari R .等。43: 521-529, 2018.
应用毒理学杂志。36: 1025-1037, 2016.
山口h等毒物科学。135: 347-355, 2013.
生物科学和生物工程杂志。91: 214-217, 2013
Takezawa T .等人的体外毒理研究。25: 1237-1241, 2011.
细胞移植。13: 463-473, 2004.