明胶酶谱试剂盒(COSMOBIO)


产品编号 产品名称 产品规格 产品等级 产品价格
PMC-AK47-COS 明胶酶谱试剂盒(COSMOBIO) 
Gelatin Zymography Kit (Cosmo Bio type)
1KIT
PMC-AK48-COS 预制明胶酶谱凝胶(COSMOBIO) 
Precast Gelatin Zymogram Gel (Cosmo Bio type)
5PLATE
PMC-AK50-COS 预制明胶酶谱凝胶(COSMOBIO) 
Precast Gelatin Zymogram Gel (Cosmo Bio type)
10PLATE
PMC-AK38-COS MMP标记 
MMP Marker
200UL
PMC-AK45-COS 明胶酶谱试剂盒(ATO) 
Gelatin zymography kit (ATTO type)
1KIT
PMC-AK46-COS 预制明胶酶谱凝胶(ATO) 
Precast Gelatin Zymogram Gel (ATTO type)
5PLATE
PMC-AK49-COS 预制明胶酶谱凝胶(ATO) 
Precast Gelatin Zymogram Gel (ATTO type)
10PLATE

明胶酶谱试剂盒(COSMOBIO)明胶酶谱试剂盒

Gelatin Zymography Kit


◆背景

明胶酶谱试剂盒(COSMOBIO)

  基质金属蛋白酶(MMP)属于金属蛋白酶家族,由至少20个成员组成,MMP参与细胞外基质蛋白的代谢。MMP常用酶谱法检测。明胶酶谱试剂盒(Cat.No.AK47-COS和AK45-COS)提供了用于酶谱电泳的简单系统。该产品用于检测血液,体液,分泌物,细胞裂解液,细胞培养基和其他样品中的ProMMP-2,MMP-2和ProMMP-9。


◆试剂盒组成


组成

包装

保存

Precast gel for 12 samples

5 pieces

4C

电泳缓冲液 (×10)

100 mL × 2

4C

洗涤缓冲液 (×10)

100 mL × 1

4C

反应缓冲液 (×10)

25 mL × 1

4C

样品制备缓冲液

5 mL × 1

4C

染色液

100 mL × 1

室温

MMP markers (ProMMP-2, MMP-2, ProMMP-9)

0.2 mL

4C

保质期:自生产日期起6个月。

凝胶规格

COSMO BIO 型: 玻璃板凝胶大小是100(W)×100(H),厚度为1mm.
              1个试剂盒中包含60个样品量的试剂。
ATTO 型玻璃板凝胶大小是120(W)×100(H),厚度为1mm.
     1个试剂盒中包含60个样品量的试剂。

该试剂盒中的预制胶与以下产品兼容

  Cosmo Bio 电泳系列: i-MyRun.P
  Life technologies: Novex
  BIO CRAFT: 电泳系统等

◆应用实例

明胶酶谱试剂盒(COSMOBIO)
  Fig.1 上样10ul MMP marker在37℃下酶反应24小时。

 

相关资料


明胶酶谱试剂盒(COSMOBIO)

欲了解详情请点击图片


◆操作步骤

1.  Load 100-150 mL of Electrophoresis Buffer to the lower chamber (anode). Refer to the instruction manual of your electrophoresis tank/chamber to know appropriate volume of the   buffer.
2.  Take out the comb on precast gel carefully and set the gel to the electrophoresis chamber. The side of the sample holes should be set on the upper side. If the sample holes are disturbed, fix them up with a needle etc.
3.  Load approximately 100 mL of Electrophoresis Buffer to the upper chamber (cathode).
4.  Mix samples with equivalent volume of sample preparation buffer. Incubate for 15 minutes at Room Temperature. (Do NOT heat the samples.)
5.  Load approximately 100 mL of Electrophoresis Buffer to the upper chamber (cathode).
6. 

Apply the samples and MMP markers to gel plate. (Fig.1: 10ml of MMP markers applied and enzymatic reacted for 24 hrs. at 37°C)  Run electrophoresis at 15 mA constant current. (If you use 2 gels, set the current at 30 mA)

*MMP markers can be used without the sample preparation buffer. 

7.  After the run is completed, turn off the electrophoresis chamber. And take out the gel plate from the chamber.
8.  Remove the upper glass plate of the gel plate and peel off the gel carefully with a spatula.
9.  Put the gel in the tray with 200 mL of Washing Buffer. Incubate with shaking at Room Temperature.
10.  Put the gel in the container with 50 mL of Reaction Buffer and seal up the container. Incubate the gel in the container in incubator at 37°C for 20-40 hrs (Lower enzyme concentration needs longer reaction time.).
11.  After the enzymatic reaction, put the gel in the container with Staining solution. Incubate for 30 minutes at Room Temperature to stain protein.
12. 

Put the gel in the container with De-staining solution. And incubate for 30minutes several hours to de-stain.

◆细胞分析相关研究用试剂盒


产品编号 产品名称 简介

PMC-AK04F-COS

TRAP Staining Kit

抗酒石酸酸性磷酸酶染色试剂盒(TRAP)

TRAP染色试剂盒用于破骨细胞中酒石酸盐抗性酸性磷酸酶的染色试剂盒。

PMC-AK02-COS

Stains All Gel Staining Kit for Acidic Proteins

酸性蛋白凝胶染色试剂盒

该试剂盒专门用于染色调节骨钙化的强酸性蛋白质,如骨钙素,骨桥蛋白和BSP II。

PMC-AK19F-COS

Adipocyte Fluorescent Staining Kit

脂肪细胞荧光染色试剂盒

用Bodipy染色和H33258的细胞核荧光染色细胞中的脂肪球。

PMC-AK11F-COS

Pancreatic beta-cell Fluorescent Staining Kit

胰腺β细胞染色试剂盒

PMC-AK03-COS

Acidic Mucopolysaccaride Assay Kit

酸性 黏多糖检测试剂盒

PMC-AK06-COS

DNA Quantity Kit

DNA定量试剂盒

无需纯化可从培养细胞定量DNA。

PMC-AK37-COS

TypeI collagenase assay kit I

型胶原酶检测试剂盒

PMC-AK09F-COS

Lipid Assay Kit

血脂检测试剂盒

该试剂盒(油红O亲脂红色染料)染色细胞内油滴,并能够用有机溶剂萃取法定量。

PMC-AK01-COS

GPDH Assay Kit

甘油磷酸脱氢酶检测试剂盒

与常规方法相比,该试剂盒在测量酶活性方面具有数个优点,包括更高的稳定性和再现性。

PMC-AK20-COS

Alkaline Phosphatase Staining Kit

碱性磷酸酶染色试剂盒

用于成骨细胞中碱性磷酸酶的染色。

PMC-AK21-COS

Calcified nodule Staining kit

钙化结节染色试剂盒

PMC-AK12-COS

POLARIC

活细胞溶致变色荧光

PMC-AK47-COS

Gelatin zymography kit

明胶酶谱试剂盒(COSMOBIO)

PMC-AK38-COS

MMP Marker

MMP标记

参考文献


[1] Miyazaki, K.,Ohta, Y., Nagai, M., Morimoto, N., Kurata, T., Takehisa, Y., Ikeda, Y.,Matsuura, T., Abe, K. Disruption of Neurovascular Unit Prior to Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis. J. Neurosci. Res.89, 718-728 (2011)
[2] Fujiwara, M.,Kashima, T. G., Kunita, A., Kii, I., Komura, D., Grigoriadis, A. E., Kudo,A., Aburatani, H., Fukayama, M. Stable Knockdown of S100A4 Suppresses CellMigration and Metastasis of Osteosarcoma. Tumour Biol.
[3] Aoki, T.,Kataoka, H., Ishibashi, R., Nozaki, K., Hashimoto, N. Simvastatin Suppresses the Progression of Experimentally Induced Cerebral  Aneurysms in Rats. Stroke. 39, 1276-85 (2008)
[4] Kuramochi,D., Unoki, H., Bujo, H., Kubota, Y., Jiang, M., Rikihisa, N., Udagawa, A.,Yoshimoto, S., Ichinose, M.,Saito, Y. Matrix  Metalloproteinase 2 Improves The Transplanted Adipocyte Survival in Mice. Eur. J. Clin. Invest. 38,752-759 (2008)
[5] Fujimoto, M.,Takagi, Y., Aoki, T., Hayase, M., Marumo, T., Gomi, M., Nishimura, M.,Kataoka, H., Hashimoto, N., Nozaki, K. Tissue Inhibitor of Metalloproteinases Protect Blood-brain Barrier Disruption in Focal Cerebral Ischemia. J.Cereb. Blood Flow Metab. 28, 1674-1685 (2008)
仅用于研究。不得用于人类基因治疗或临床诊断。

乙酸[GK法]检测试剂盒 Acetic Acid GK Assay Kit (Analyser Format) 货号:K-ACETGK Megazyme中文站

乙酸[GK法]检测试剂盒

英文名:Acetic Acid GK Assay Kit (Analyser Format)

货号:K-ACETGK

规格:110 mL of prepared reagent (e.g. 500 assays of 0.22 mL)

The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.

As part of Megazyme’s overall commitment to providing the highest quality products, we have developed this acetic acid kit that provides a specific and rapid assay for use with auto-analysers. The kit assay is based on the conversion of NAD+ to NADH and therefore provides a positive reaction (increase in absorbance) which offers a more robust assay.

The reagents, as supplied, are stable for a minimum of 2 years and the prepared reagents are stable for a minimum of 1 week (on-board stability). In addition, the prepared reagents can be stored frozen for longer term stability

Q1. Is the acetic acid kit specific for acetate?

Propionate may react more slowly than acetate.

Q2. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q3. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q4. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q5. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q6. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q7. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q8. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q9. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q10. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q11. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q12. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

Nipponham 胶原系列


产品编号 产品名称 产品规格 产品等级 产品价格
309-31595 Porcine Skin Collagen Solution
猪皮胶原溶液
500g
 300-31601  Chicken Cartilage Collangen Solution
鸡软骨胶原溶液
100g
307-31611 Porcine Skin Collagen TypeⅠSolution
猪皮胶原Ⅰ溶液
100g
301-84621 NMP Collangen PS
NMP胶原PS
100g

Nipponham 胶原系列Nipponham 胶原系列


追求品质、安全性、便利性的Nipponham胶原



◆特点


 独创技术 品质保证

     本产品已使用胃蛋白酶去除端肽,具有可溶性。生产管理技术先进,批次间无物理特性差距。

 

 现成产品 使用方便

     浓度已调制为制作Scaffold的最适合浓度1%。已经过灭菌处理,节省时间。

     *:0.45μm 滤过灭菌


 Nipponham独有的高安全性

     产品原料来自Nipponham的农场,农场对动物进行严格饲育管理。生产过程中包含病毒灭活处理,请放心使用。


Nipponham 胶原系列
     猪皮胶原溶液                            鸡软骨胶原溶液                           猪皮胶原Ⅰ溶液



胶原类型 Ⅰ型及Ⅲ型 Ⅱ型 Ⅰ型
来源(原产国) 猪皮(日本) 鸡剑状软骨(日本) 猪皮(日本)
包装 500g

100g

100g
浓度 1% 1% 1%
溶剂 盐酸水溶液 盐酸水溶液 盐酸水溶液

 

 


<NMP collagen PS>

 

       图为具有优良操作性及溶解性的散包装猪皮胶原。NipponhamNipponham 胶原系列

生产管理技术先进,生产过程中包含病毒灭活处理,请放心使用。


 


 

 

 

 

 

 

加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售

产品详细

科学仪器

加热块部1 L用300℃规格

加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售

  • 加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售

商品代码其他情报(式样)

这个产品比较表中追加
产品照片 加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售
加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售
商品代码 054300 – 3510
型式
价格(不含税) 498,1000日元

上海金畔生物科技有限公司

513%2F%3Fc%3D34& ” 513%2F%3Fc%3D34&

HC-067047


产品编号 产品名称 产品规格 产品等级 产品价格
085-10281 HC-067047 10mg 细胞生物学用

TRPV4拮抗剂

【New】HC-067047


  HC-067047是TRPV4(Transient Receptor  Potential Vanilloid 4)的强力拮抗剂。实验发现,TRPV4在低渗透压中是活性化的渗透压感受性和温度感受性受体。TRPV4还与皮肤的屏障功能有关。

  HC-06704对TRPV1,V2,V3的抑制活性比对TRPV4的至少要高出100倍。对hEGF和TRPM8的抑制活性约为对TRPV4活性的十分之一。

 


◆ 产品简介


 ● 外观:白色~淡褐色、结晶性粉末~粉末;

 ● 含量(HPLC):98.0%以上

 ● 乙醇溶解状态:实验适用

HC-067047

◆ 相关产品:

 


TRPV4激动剂


产品编号

产品名称

规格

容量

073-06491

GSK1016790A
强力的TRPV4激动剂。
是4α-PDD作用的300倍

细胞生物学用

5mg

079-06493

25mg

166-26311

4α-Phorbol 12,13-Didecanoate【4α-PDD】
TRPV4激动剂。也用作Phorbol 12,13-didecanoate的阴性对照品。

细胞生物学用

1mg

162-26313

5mg

183-02981

RN-1747
TRPV4激动剂。
对TRPM8有拮抗作用。

细胞生物学用

10mg

189-02983

50mg

 

TRPV4拮抗剂


产品编号

产品名称

规格

容量

189-03181

Ruthenium Red
对TRPA1,M6,V1,V2,V3,V4,V5,V6有拮抗作用。

和光一级

100mg

185-03183

1g

 

TRPV4抗体


产品编号

产品名称

规格

容量

305-33611

Anti Mouse TRPV4 Polyclonal Antibody

10μl/vail

合成·反应装置ケミスト广场CPG – 2000 / CPP – 2000系列用搅拌孩子椭圆形边缘型5个入|柴田科技有限公司-环境检测设备、科学仪器的制造销售

产品详细

科学仪器

合成·反应装置ケミスト广场CPG – 2000 / CPP – 2000系列用搅拌孩子椭圆形边缘型5个入

商品代码其他情报(式样)

这个产品比较表中追加
商品代码 054310 -点A
型式
价格(不含税) 8,500日元

上海金畔生物科技有限公司

648%2F%3Fc%3D34& ” 648%2F%3Fc%3D34&

4-硝基苯基-β- laminaritetraosid 4-Nitrophenyl-β-laminaritetraoside 货号:O-PNPLAM4 Megazyme中文站

4-硝基苯基-β- laminaritetraosid

英文名:4-Nitrophenyl-β-laminaritetraoside

货号:O-PNPLAM4

规格:10 mg

Synonyms: p-Nitrophenyl-β-laminaritetraoside, 
                   pNP-β-laminaritetraoside
CAS: 106678-65-3
Molecular Formula: C30H45NO
23
Molecular Weight: 787.7
Purity: > 98%

High purity 4-Nitrophenyl-β-laminaritetraoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement/detection of 1,3-β-glucan degrading enzymes.

暂无问题解答

暂无视频

Amresco甘氨酸GLYCINE

Amresco甘氨酸GLYCINE 

Grade:BIOTECHNOLOGY GRADE

CAS Number:56-40-6

Synonym(s):aminoethanoic acid, aminoacetic acid

Molecular Weight:75.07

Molecular Formula:C2H5NO2

Storage Condition:ROOM TEMPERATURE

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