Tacsimate pH 4, 5, 6, 7, 8, & 9蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Tacsimate pH 4, 5, 6, 7, 8, & 9
Tacsimate pH 4, 5, 6, 7, 8, & 9

Tacsimate pH 4, 5, 6, 7, 8, & 9

Tacsimate pH 4, 5, 6, 7, 8, & 9

Tacsimate pH 4, 5, 6, 7, 8, & 9

Tacsimate pH 4, 5, 6, 7, 8, & 9

Products > Optimize Reagents > Optimize – Salts > Tacsimate pH 4, 5, 6, 7, 8, & 9

Tacsimate pH 4, 5, 6, 7, 8, & 9

Applications

  • Crystallization grade Tacsimate for formulating screens or for optimization

Features

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

To screen pH as a crystallization variable using Tacsimate one can mix together different ratios of different pH Tacsimate solutions. Refer to the pdf “Mixing Tacsimate Stocks to Screen pH” on this page. Or, one can add a buffer to the crystallizaiton reagent containing Tacsimate. Recommended final buffer concentration in the reagent well is 0.05 to 0.1 M.

Tacsimate
A pH titrated mixture of organic acids. 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic. pH adjusted using NaOH

Measured pH of 100% Tacsimate is 4.0 at 25°C
Measured pH of 100% Tacsimate is 5.0 at 25°C
Measured pH of 100% Tacsimate is 6.0 at 25°C
Measured pH of 100% Tacsimate is 7.0 at 25°C
Measured pH of 100% Tacsimate is 8.0 at 25°C
Measured pH of 100% Tacsimate is 9.0 at 25°C

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Tacsimate pH 4, 5, 6, 7, 8, & 9

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Tacsimate pH 4, 5, 6, 7, 8, & 9

Tacsimate pH 4, 5, 6, 7, 8, & 9

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Tacsimate pH 4, 5, 6, 7, 8, & 9

CAT NO

HR2-823

NAME

100% Tacsimate pH 4.0

DESCRIPTION

200 mL

PRICE

$120.00

cart quote

CAT NO

HR2-825

NAME

100% Tacsimate pH 5.0

DESCRIPTION

200 mL

PRICE

$120.00

cart quote

CAT NO

HR2-827

NAME

100% Tacsimate pH 6.0

DESCRIPTION

200 mL

PRICE

$120.00

cart quote

CAT NO

HR2-755

NAME

100% Tacsimate pH 7.0

DESCRIPTION

200 mL

PRICE

$120.00

cart quote

CAT NO

HR2-829

NAME

100% Tacsimate pH 8.0

DESCRIPTION

200 mL

PRICE

$120.00

cart quote

CAT NO

HR2-813

NAME

100% Tacsimate pH 9.0

DESCRIPTION

200 mL

PRICE

$120.00

cart quote

Support Material(s)

Tacsimate pH 4, 5, 6, 7, 8, & 9 What is Tacsimate?Tacsimate pH 4, 5, 6, 7, 8, & 9 Mixing Tacsimate Stocks to Screen PhTacsimate pH 4, 5, 6, 7, 8, & 9 HR2-823 Tacsimate 100% solution pH 4.0 SDSTacsimate pH 4, 5, 6, 7, 8, & 9 HR2-825 Tacsimate 100% solution pH 5.0 SDSTacsimate pH 4, 5, 6, 7, 8, & 9 HR2-827 Tacsimate 100% solution pH 6.0 SDSTacsimate pH 4, 5, 6, 7, 8, & 9 HR2-755 Tacsimate 100% solution pH 7.0 SDSTacsimate pH 4, 5, 6, 7, 8, & 9 HR2-829 Tacsimate 100% solution pH 8.0 SDSTacsimate pH 4, 5, 6, 7, 8, & 9 HR2-813 Tacsimate 100% solution pH 9.0 SDS

Certificate Of Analysis

References

1. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology 156 (2006) 387–406.

2. A novel strategy for the crystallization of proteins: X-ray diffraction validation. Steven B. Larson, John S. Day, Robert Cudney and Alexander McPherson. Acta Cryst. (2007). D63, 310–318.

3. Structure of the catalytic and ubiquitin-associated domains of the protein kinase MARK/Par-1. Eckhard Mandelkow et al. Structure 14, 173-183, February 2006.

4. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

5. Crystallization of a newly discovered histidine acid phosphatase from Francisella tularensis. Richard L. Felts, Thomas J. Reilly, Michael J. Calcutt, and John J. Tanner. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 January 1; 62(Pt 1): 32–35.

6. Structure of the GTPase and GDI domains of FeoB, the ferrous iron transporter of Legionella pneumophila. Petermann et al, FEBS Letters Volume 584, Issue 4, Pages 733-738 (19 February 2010).

Tacsimate pH 4, 5, 6, 7, 8, & 9 Tacsimate pH 4, 5, 6, 7, 8, & 9

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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