英文名：Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)
规格：100 assays (manual) / 400 assays (auto-analyser)
100 assays (manual format) / 400 assays (auto-analyser format)
100 / 200 assays (manual format) / 330 assays (auto-analyser format)
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase. Mixed linkage
β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 188.8.131.52) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g.Trichoderma sp.) but this does not result in an increase in absorbance.
Data calculators are located in the Documentation tab.
Catalase (Aspergillus niger)
catalase; hydrogen-peroxide:hydrogen-peroxide oxidoreductase
In 3.2 M ammonium sulphate.
Supplied at ~ 18,000 U/mL.
> 2 years at 4oC.
~ 4,960 U/mg protein (using A240 method) at pH 7.0 and 25oC.
One Unit of catalase activity will decompose 1 micromole of H2O2 per minute at pH 7.0 and 25oC, while the H2O2 concentration falls from 10.3 mM to 9.2 mM. The rate of disappearance of H2O2 is followed by observing the rate of decrease in the absorbance at
240 nm (A240).
Decomposition of hydrogen peroxide into water and oxygen.
For use in research.