Nuclease P1


产品编号 产品名称 产品规格 产品等级 产品价格
145-08221 Nuclease P1
核酸酶P1
500 U 基因研究用

核酸酶P1Nuclease P1

Nuclease P1

基因研究用 for Genetic Research

制造商:FUJIFILM Wako Pure Chemical Corporation

储存条件:冷藏(冷藏运输)

CAS RN®54576-84-0



◆概况

核酸酶P1分解RNA和热变性DNA的3'-5'-磷酸二酯键。另外,核酸酶P1还分解单核寡核苷酸的3'-磷酸单酯键。如果的氯化钠(pH 6.0)含量超过400mmol/L,它不作用于双链DNA。

◆应用实例

A.

Sample : 200 μL of RNA or heat-denatured DNA at a density of 2 mg/mL.

B.

Reaction Buffer : 200 μL of 0.01 mol/L barbital(veronal) acetate buffer, pH 5.3*.

C.

Enzyme : 100 μL of 100 units/mL of Nucelase P1 solution with distilled water.

D.

top Solution : 500 μL of cold uranyl acetate buffer (0.25% of uranyl acetate and 2.5% perchloric acid)

*barbital (veronal) acetate buffer, pH 5.3: Prepare 0.01 mol/L Barbital Sodium Salt in water and adjust it to pH 5.3 with acetic acid.

1. Mix each 200 μL of A, B and C.

2. Incubate it at 50 degree C for 1 hour.

3. The reaction can be stopped by adding 500 μL of D.

◆参考文献

Masao Fujimoto, Akira Kuninaka & Hiroshi Yoshino(1973)Purification of a Nuclease from Penicillium citrinum, Agr. Biol. Chem.,38(4), 777-783.



◆用途

用于核酸相关的研究。

◆相关信息


外观:冻干品

来源:Penicillium citrinum

活性:约500 units/vial(3'-5'-磷酸二酯酶活性)

pH信息:最佳pH:5.3(RNA,热变性DNA),7.2(3'-AMP)

温度:最佳温度:约70°C,60°C(长时间孵育时)

 热稳定性:60°C以下(pH 5.3,30 min)稳定。