Dialysis Buttons蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Dialysis Buttons
Dialysis Buttons

Dialysis Buttons

Dialysis Buttons

Dialysis Buttons

Dialysis Buttons

Products > Crystallization Plates, Hardware & Accessories > Dialysis Buttons, Membranes & Applicators > Dialysis Buttons

Dialysis Buttons

Applications

  • Crystallization by dialysis, protein folding, & small volume sample dialysis

Features

  • Low volume dialysis
  • Fits in 24 well plates
  • Use over and over again

Description

In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3

Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.

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Dialysis Buttons

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Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons

Dialysis Buttons

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Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons

CAT NO

HR3-336

NAME

Dialysis Buttons Sampler

DESCRIPTION

5 of each size listed below

PRICE

$105.00

cart quote

CAT NO

HR3-314

NAME

5 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

cart quote

CAT NO

HR3-316

NAME

10 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

cart quote

CAT NO

HR3-318

NAME

15 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

cart quote

CAT NO

HR3-320

NAME

20 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

cart quote

CAT NO

HR3-326

NAME

50 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

cart quote

CAT NO

HR3-328

NAME

100 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

cart quote

CAT NO

HR3-330

NAME

200 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

cart quote

CAT NO

HR3-332

NAME

350 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

cart quote

Support Material(s)

Dialysis Buttons Dialysis Button User Guide

Related Item(S)

  • Dialysis Membrane Discs for Buttons

References

1. McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).

2. Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).

3. Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.

4. Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.

Dialysis Buttons Dialysis Buttons

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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