康宁corning 3897 PLT,96WL,VB,WO/LID,NS,BK,25/10 25/包 4包/箱 1435.74
日度归档:2025年1月4日
抗细胞角蛋白19抗体[ ep1580y ](ab52625)
种属反应性
靶标
功能 参与肌纤维组织。 连同KRT8,帮助链接收缩装置的抗肌萎缩蛋白在横纹肌costameres。 组织特异性 在深外根鞘的毛囊中定义的基底细胞区的表达。 在汗腺和乳腺导管和分泌细胞、胆管、胃肠道、膀胱尿路上皮、口腔黏膜、食管也观察到,宫颈上皮细胞(蛋白)。 在表皮基底细胞中表达,在乳头表皮和毛囊的区域定义。 在叶脉和脐带动脉血管壁细胞的一个子集也看到,和脐带血管平滑肌。 肌纤维的肌浆中积累的costameres在含有抗肌萎缩蛋白与血影蛋白膜结构观察。 序列相似性 属于中间丝家族。 发展阶段 在各个发展阶段的毛囊中存在。 结构域 这种蛋白不同于所有其他如果缺乏C末端结构域的蛋白质。 -
四乙酰壳四糖 Tetraacetyl-Chitotetraose – 20mg 货号:O-CHI4 Megazyme中文站
四乙酰壳四糖
英文名:Tetraacetyl-Chitotetraose – 20mg
货号:O-CHI4
规格:20 mg
CAS: 2706-65-2
Molecular Formula: C32H54N4O21
Molecular Weight: 830.4
Purity: 95%High purity Tetraacetyl-chitotetraose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Prepared from chitin.
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Whatman 滤膜 6876-2504 GD/X 25针头式滤器0.45m聚砜 150/盒
Whatman 滤膜 6876-2504 GD/X 25针头式滤器0.45m聚砜 150/盒 GD/X 25/0.45 PES 150/PK
和光纯药WAKO 012-08643 Amino Acids Mixture Standard Solution, Type B 1mLx5
和光纯药WAKO 012-08643 Amino Acids Mixture Standard Solution, Type B for Amino Acid Automated Analysis –
Pall颇尔滤膜 12035-028 MEP HYPERCEL BIOSEPRA 100ML
Pall颇尔滤膜 12035-028 MEP HYPERCEL BIOSEPRA 100ML 800 6403
Axygen MTS-11-12-C-R-S 1.1ml迷你试管12联排 8排/盒,10*5盒/箱
Axygen MTS-11-12-C-R-S 1.1ml迷你试管12联排 8排/盒,10*5盒/箱 3940.71
胶体滴定试剂(聚乙烯硫酸钾-PVSK)
胶体滴定试剂(聚乙烯硫酸钾-PVSK)
胶体滴定(colloid titration) 是一种简单、快速、准确的测定高分子聚合物电荷的方法。Potassium Polyvinyl Sulfate(聚乙烯硫酸钾,PVSK)于水中可形成带负电的胶体颗粒,Glycol Chitosan(GC)或Methyl Glycol Chitosan(MGC)在酸性水溶液中带正电,利用电性中和的原理,负电胶体颗粒的会与正电胶体颗粒反应达到电性中和。在适当的pH条件下,此两种胶体可完全反应形成不溶性沉淀物。使用甲苯胺蓝(Toluidine Blue,TB)为指示剂,与正电性胶体混合时,两者之间没有反应,TB维持蓝色;当悬浮液中胶体带负电时,TB会吸附于胶体表面而现紫色。
滴定时,正电胶体可直接以PVSK溶液为滴定液,TB为指示剂进行滴定;对于负电荷胶体,可以直接以Glycol Chitosan 或MGC滴定,或加入已知的过量的正电胶体,再以PVSK溶液对剩余的正电胶体进行反滴定。
阴离子试剂:
货号 品名 包装 164-21655 Potassium Polyvinyl Sulfate Titration Solution (N/400) 聚乙烯硫酸钾(PVSK)滴定液 500ml 现货供应 162-03071 Potassium Polyvinyl Sulfate聚乙烯硫酸钾(PVSK) 10g 阳离子试剂:
货号 品名 包装 072-05045 Glycol Chitosan Titration Solution (N/200)
乙二醇壳聚糖滴定液500ml 072-01581 Glycol Chitosan
乙二醇壳聚糖10g 137-14655 Methyl Glycol Chitosan Titration Solution(N/200)
甲基乙二醇壳聚糖滴定液500ml 134-04731 Methyl Glycol Chitosan
甲基乙二醇壳聚糖10g 161-14695 0.0025N Poly(diallyldimethylammonium Chloride) Solution
聚二甲基二烯丙基氯化铵滴定液500ml 指示液:
货号 品名 包装 205-05811 Toluidine Blue Indicator Solution甲苯胺蓝指示液 50ml PVSK标定试剂:
货号 品名 包装 080-06681 Cetylpridinium chloride monohydrate氯化十六烷基吡啶 10g 086-06683 Cetylpridinium chloride monohydrate氯化十六烷基吡啶 100g 上海金畔生物科技有限公司
地 址: 上海市浦东新区东靖路699弄36栋701室
邮 编: 201208
固话总机:
订货热线:15221999938
24小时短信服务:15221999938
服务投诉:15221999938
网 址: www.jinpanbio.com
Email:sales@jinpanbio.com;
Megazyme 直链淀粉试剂盒K-AMYL说明书
前言:
谷类淀粉的许多特性决定其最终用途,这些取决于直链淀粉/支链淀粉的比率。这些特性包括糊化和凝胶化,溶解度,抗性淀粉的形成以及整颗大米的烹饪和构造特性。因此,淀粉中直链淀粉含量的测定是淀粉加工的一个重要的质量参数。
最常用的测定谷物淀粉中直链淀粉含量的方法是利用电势,电流测定或直链淀粉的碘结合能力比色测定直链淀粉-碘色合配合物。然而,这些方法具有不确定性。支链淀粉-碘复合物也可以形成,这样降低了利用非比色法测定的游离碘离子的浓度,并且用比色法测量时,该复合物可能和直链淀粉-碘复合物吸收相同波长的光。这种复合物致使直链淀粉的测定含量超过实际含量,需要进行校正。Gibson等详细列举了使用这些方法所遇到的许多其他问题。
支链淀粉结合ConA的特殊复合物为淀粉中直链淀粉的测定提供了一种替代方法,而且不存在不确定性问题。在指定pH值,温度和离子强度的条件下,ConA特异性结合分支多糖并形成沉淀,这种结合以多个非还原性末端基团上的α-D-吡喃葡萄糖基或α-D-吡喃甘露糖基单位为基础。因此,ConA可以有效结合淀粉中的支链淀粉成分,但是不能结合线性为主的直链淀粉成分。
此方法是Yun和Matheson改进的ConA方法。分析之前用乙醇预处理去除脂质。
原理:
淀粉样品通过加热完全地溶解在二甲基亚砜(DMSO)里。用乙醇沉淀淀粉去除其中的脂质,回收沉淀的淀粉。用醋酸/盐溶液溶解沉淀的样品,加入ConA,特异性沉淀支链淀粉,离心去除沉淀。单位体积上清液中的直链淀粉用酶水解为D-葡萄糖,然后用葡萄糖氧化酶/过氧化物酶试剂进行测定。另外一份单位体积醋酸/盐溶液中的总淀粉同样用酶水解为D-葡萄糖,然后加入葡萄糖氧化酶/过氧化物酶,用比色法测定。
根据ConA沉淀样品的上清液和与总淀粉样品中的GOPOD在510 nm处的吸光光度值之比判断直链淀粉在总淀粉中的含量。
该方法适用于所有的纯淀粉和谷物粉。
精确性
样品如为纯淀粉,相对标准偏差为<5%。
样品如为谷物面粉,相对标准偏差为~10%。试剂盒:
瓶子1:冻干的Con A (伴刀豆球蛋白 A concanavalin A) ,200 mg,-20℃下稳定性>5年。
瓶子2:淀粉葡糖苷酶【200U,条件为消化对硝基苯基β-麦芽糖苷(也就是3300U,条件为pH4.5,40℃下消化淀粉)】加上真菌α-淀粉酶(500U ,条件为pH5.0,40℃下消化 Ceralpha 试剂),2mL,4℃下稳定性>5年。
瓶子3:GOPOD 试剂缓冲液。磷酸钾缓冲液(1M,pH7.4),对羟苯甲酸(0.22M)和叠氮化钠(0.02% W/W)。4℃下稳定性> 3年。
瓶子4:GOPOD试剂酶。葡糖氧化酶(>12,000U)加上过氧化物酶(>650U)和4-氨基安替比林(80mg)。冻干粉,-20℃下稳定性>5年。
瓶子5:D-葡萄糖标准溶液(5 mL,1.0mg/mL)溶于苯甲酸0.2%(w/v)。室温下稳定性>5年。
瓶子6:淀粉参考样品(含有特定含量的直链淀粉),室温下稳定性>5年。试剂制备的准备:1、用50mlCon A试剂将瓶子1中药品溶解,分成适当的几部分存放在聚丙烯管中,尽可能在-20℃下使用或冷藏。-20℃下稳定性>2年。
2、用20ml醋酸钠缓冲液将瓶子2中的药品溶解,分成适当的几部分存放在聚丙烯管中,尽可能在-20℃下使用或冷藏。-20℃下稳定性>2年。3、用蒸馏水将中的药品(GOPOD 试剂缓冲液)稀释至1L。现配现用。(注意:如果瓶子3意外存放在-20℃下,一些盐可能会结晶。配制时要确保所有的结晶物质都溶解在1L的蒸馏水中)4、用20ml GOPOD 试剂缓冲液溶解GOPOD试剂酶,并定量的转移到存放GOPOD 试剂缓冲液的瓶子中,用铝箔封住瓶子,避光保存。这种试剂黑暗中保存,2-5℃下能保存大约3个月,-20℃下稳定性大于12个月。5、按提供的方法使用D-葡萄糖标准溶液和淀粉参考样品,室温下稳定性>5年。安全考虑:1、DMSO对皮肤有刺激性,用的时候要格外小心。它通过皮肤被吸收,对皮肤和眼睛都有刺激性。穿保护装、戴手套,避免溶剂溅出。尽可能在通风橱中进行试验。2、ConA通过呼吸、皮肤接触或摄入体内,对人体是十分有害的。这种伤害不可逆转,可能会致畸。在使用ConA结晶体球蛋白和含有ConA的溶剂时要穿保护装、戴手套和特制的面具。3、叠氮化钠是有毒的化学试剂,必须要有专门的处理。它要添加特殊的缓冲液来充当防腐剂。毒性通过缓冲可以被消除,但是缓冲液必须在4℃下保存。缓冲液或溶剂:1、乙酸钠缓冲液(10mM,pH 4.5)加5.9ml1.05g/ml的冰醋酸到900ml的蒸馏水中用4g/100ml的NaOH溶液调pH到4.5(大约30ml),加0.2g叠氮化钠,用蒸馏水定容至1L。室温下稳定性>2年。2、ConA 浓缩液(600 mM, pH 6.4 乙酸钠缓冲液)称取49.2g无水醋酸钠,175.5g氯化钠,0.5gCaCl2.2H2O ,0.7 gMgCl2.6H2O 和0.7 g MnCl2.4H2O ,用900 mL 蒸馏水将其溶解逐滴加入冰醋酸调pH为6.4,再用蒸馏水定容至1L。4℃下保存2周。(注意:配制这种复合缓冲液时,pH的调节特别重要,如果pH低于6.4,就会有沉淀物形成,就算pH再调回来,沉淀物也不会溶解。结果会导致缓冲液必须丢弃重新配制。)3、ConA 溶液(使用浓度)将30ml ConA 浓缩液用蒸馏水稀释至100ml。现配现用。4、DMSO分析纯试剂。室温下可保存5年。预处理:淀粉样品事先用乙醇进行预处理,除去脂质。如果样品没有用乙醇进行预处理,在某些样品中测得的直链淀粉含量会低50%。实验步骤:A、淀粉预处理1、准确称取淀粉或面粉样品至10ml的样品管中。记录样品重量精确到0.1mg。2、加1ml DMSO至试管,在漩涡混合器中慢速混匀,在沸水浴中加热直至分散(大约1分钟)确保淀粉没有结团结块。3、密封试管,高速混匀。沸水浴加热15min,再高速间歇混匀。4、室温放置大约5min,加2ml 95%乙醇,混匀,再加4ml乙醇,盖上盖子,颠倒混匀。形成淀粉沉淀物,将试管静止15min(条件允许过夜静止)。5、2000r,5min离心,弃去上清液,倒置在纸巾上10min,确保乙醇全部挥发。在接下来直链淀粉和淀粉的测定中使用此沉淀物。6、加2ml DMSO到淀粉沉淀物中,将试管沸水浴15min(间或混匀),确保没有块状。7、从沸水浴中取出试管,立即加入4ml ConA 溶液,将试管中药品转移至25ml容量瓶中。用 ConA 溶液定容。(整个过程必须在2h内完成)B、支链淀粉- ConA 沉淀物和直链淀粉的测定1、量取1mlA试剂到2ml的试管中,加0.54ml的ConA 溶液,盖上盖子,反复颠倒,混匀。避免样品起泡。2、室温静置1h。14000r,10min,室温下离心。3、转移1ml的上清液到15ml的离心管中,,加3ml100mM醋酸钠缓冲液,pH4.5,混合药品。轻轻塞住管口,沸水浴5min,使ConA变性。4、将试管在40℃下水浴,平衡5min,,加1ml淀粉转葡萄糖苷酶和a-淀粉酶的混合物,40摄氏度下反应30min,2000r离心5min。5、准确量取1ml上清液,加4ml GOPOD试剂,40摄氏度下反应20min,使空白试剂和D-葡萄糖标准液同时反应。6、在510nm下测定每一个样品和D-葡萄糖标准液的吸光光度值。C、总淀粉的测定1、将0.5mlA溶液和4ml100mM乙酸钠溶液混合,pH 4.52、加1ml淀粉转葡萄糖苷酶和a-淀粉酶的混合物,40摄氏度下反应10min,3、准确量取1ml上清液,加4ml GOPOD试剂,40摄氏度下反应20min。这个反应必须让样品和标准液按B部分同时进行。方形广口瓶C型
产品编号 产品名称 产品规格 产品等级 产品价格 2133 方形广口瓶C型 1L – – 2134 方形广口瓶C型 2L – – 方形广口瓶
容量
1L
2L
尺寸
82×109×167H mm
85×145×210H mm
口径
44φ mm
44φ mm
Q1: 请问本体、盖子和内盖是用什么材质制成,耐热温度分别是多少?
A1: 本体、盖子和内盖都是由聚乙烯制成,耐热温度是80℃左右。
SAFETRAN核酸载体
产品编号 产品名称 产品规格 产品等级 产品价格 CSR-KMU-T01 SAFETRANS ((ALPHA)-CDE)
核酸载体2mg – – CSR-KMU-T02 SAFETRANS (GUG-(BETA)-CDE)
核酸载体2mg – – CSR-KMU-T03 SAFETRANS (Lac-(ALPHA)-CDE)
核酸载体2mg – – 体内、体外转染试剂盒
SAFETRANS核酸载体
◆原理
SAFETRANS是一种特殊的基因载体,由树枝状聚合物(阳离子聚合物)和环状低聚糖组成,能够在体内和体外将pDNA、shRNA、siRNA、hRNA和miRNA导入到多种细胞内,并且细胞毒性极小。
◆优点、特色
● 能够转染pDNA、shRNA、siRNA、hRNA和miRNA
● 通过静脉内和皮下注射能够在体内转染
● 兼容反向转(仅α-CDE)
● 成功转染多种细胞系
● 低细胞毒性
● 一致的基因诱导率
● 根据不同情况可对操作手册微调
● 肝细胞可选择性转染
D-乳酸[D-乳酸盐]检测试剂盒[快速] D-Lactic Acid (D-Lactate) (Rapid) Assay Kit 货号:K-DATE Megazyme中文站
D-乳酸[D-乳酸盐]检测试剂盒[快速]
英文名:D-Lactic Acid (D-Lactate) (Rapid) Assay Kit
货号:K-DATE
规格:50 assays (manual) / 500 assays
分析物意义:水果、蔬菜产品的质量指标
Megazyme检测试剂盒优点:反应快、试剂稳定
The D-Lactic Acid (D-Lactate) (Rapid) test kit is suitable for the rapid, specific measurement and analysis of D-lactic acid in wine, beer, juice, milk, cheese, vinegar, meat and other food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.UV-method for the determination of D-Lactic Acid in foodstuffs,
beverages and other materialsPrinciple:
(D-lactate dehydrogenase)
(1) D-Lactic acid + NAD+ ↔ pyruvate + NADH + H+(glutamate-pyruvate transaminase)
(2) Pyruvate + D-glutamate → D-alanine + 2-oxoglutarateKit size: 50 assays (manual) / 500 (microplate)
/ 450 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.21 mg/L
Application examples:
Wine, soft drinks, milk, dairy products (e.g. cream, milk / whey powder,
cheese, condensed milk and yogurt), foods containing milk (e.g. dietetic
foods, bakery products, baby food, chocolate, sweets and ice-cream),
vinegar, fruit and vegetables, processed fruit and vegetables, meat
products, food additives, paper (and cardboard), cosmetics,
pharmaceuticals and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by DIN, GOST,
IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAKAdvantages
- Very rapid reaction with most samples (~ 5 min)
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Extended cofactors stability
- Suitable for manual, microplate and auto-analyser formats
Q1. There is an issue with the performance of the kit; the results are not as expected.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
- Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
- Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
- State the kit lot number being used (this is found on the outside of the kit box).
- State which assay format was used (refer to the relevant page in the kit booklet if necessary).
- State exact details of any modifications to the standard procedure that is provided by Megazyme.
- State the sample type and describe the sample preparation steps if applicable.
Q2. Should the pH of the sample be adjusted even for samples in acidic media?
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.Q3. Can perchloric acid be used to deproteinise/clarify samples prior to analysis using the D-Lactic Acid Assay Kit (K-DATE)? If so, how should such an extraction be performed?
Yes. Perchloric acid extraction can be used in conjunction with this kit, and should be performed as follows:
WARNING: If you have not worked with perchloric acid before, you must consult your safety officer for advice. Also, depending on the nature of the samples, it may be possible to reduce the concentration of perchloric acid, to for example 0.3 M (i.e. in the case of plasma). It is thus very important to determine if this is possible for each type of sample used, in order to reduce the risk from working with concentrated perchloric acid.
Liquid samples:- Carefully add an equal volume of ice cold 3 M perchloric acid and homogenise / fully disperse the sample (as appropriate).
- After 15 min incubation on ice (or in a refrigerator), centrifuge at 3000 x g for 15 min at 4°C.
- Neutralise by the slow addition of 2 M KOH.
- Incubate on ice (or in a refrigerator) until the potassium perchlorate has settled out by gravity (approximately 10 min), and then simply remove some of the clear supernatant and use directly in the assay.
Solid samples:- Accurately weigh approx. 5 g of homogenised sample into a beaker containing 20 mL of 1 M perchloric acid and very carefully homogenise with an Ultraturrax® (or equivalent) for 5 min.
- Carefully add approx. 40 mL of distilled water and neutralise using 2 M KOH (using pH test strips for example). Quantitatively transfer the contents to a 100 mL volumetric flask and fill to the mark with distilled water. If a fat layer develops, make sure this is above the mark, and the aqueous layer is at the mark.Incubate in a refrigerator for approx. 20 min to allow separation of fat and precipitation of potassium perchlorate.
- Filter through Whatman No. 1 filter paper, discarding the first few mL of filtrate, and use directly in the assay.
Q4. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?
Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.
Q5. Is the D-Lactic Acid (D-Lactate) (Rapid) Assay Kit (K-DATE) suitable for measurement using cell culture media samples?
Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit. It may be sufficient to use the sample directly in the assay after clarification by centrifugation/filtering followed, by dilution (if required) in distilled water.
Q6. Can you explain, step by step, how to follow the method and perform the kit assay?
For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):
1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.Q7. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?
The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.
Q8. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?
Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.Q9. I have some doubts about the appearance/quality of a kit component what should be done9
If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).
Q10. Can the test kit be used to measure biological fluids and what sample preparation method should be used?
The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2
The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.Q11. Can the manual assay format be scaled down to a 96-well microplate format?
The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.
There a 3 main methods for calculation of results using the microplate format:- The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
- Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
- Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.
Q12. Can the sensitivity of the kit assay be increased?
For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.
Q13. How much sample should be used for the clarification/extraction of my sample?
The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).
Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.Pall颇尔滤膜 AP-4000 PSF25MM 10VERSAPOR AUTOPK200
Pall颇尔滤膜 AP-4000 PSF25MM 10VERSAPOR AUTOPK200 237 1896
Amresco 0394-100G LIGHT GREEN SF YELLOWISH 亮绿
Amresco 0394-100G LIGHT GREEN SF YELLOWISH 亮绿
96高纯质粒DNA小提试剂盒(TIANprep N96 Plasmid Kit)
上海金畔生物科技有限公司提供96高纯质粒DNA小提试剂盒(TIANprep N96 Plasmid Kit)
SBO物料类别 目录号 物料描述 规格 目录价
96高通量(核酸纯化) DP213-01 96 DNA 产物纯化试剂盒(TIANquick N96 Purification Kit) 4板 1,480.00
96高通量(核酸纯化) DP213-02 96 DNA 产物纯化试剂盒(TIANquick N96 Purification Kit) 24板 7,548.00
96高通量(核酸提取) DP114-01 96高纯质粒DNA小提试剂盒(TIANprep N96 Plasmid Kit) 4板 1,680.00
96高通量(核酸提取) DP114-02 96高纯质粒DNA小提试剂盒(TIANprep N96 Plasmid Kit) 24板 8,568.00
96高通量(核酸提取) DP114-03 96高纯质粒DNA小提试剂盒(TIANprep N96 Plasmid Kit) 48板 14,568.00
96高通量(核酸提取) DP115-01 96快速质粒DNA小提试剂盒(TIANprep Rapid N96 Plasmid Kit) 4板 1,280.00
96高通量(核酸提取) DP115-02 96 快速质粒DNA小提试剂盒(TIANprep Rapid N96 Plasmid Kit) 24板 6,528.00
96高通量(核酸提取) DP119 N96磁珠法质粒DNA小提试剂盒(TIANprep N96 Magnetic Plasmid Kit) 2 Plates 890.00
96高通量(核酸提取) DP314-01 96血液基因组DNA提取试剂盒(含K)(TIANamp N96 Blood DNA Kit) 4板 3,800.00
96高通量(核酸提取) DP314-T N96动物组织基因组DNA提取试剂盒 8板 52,640.00
96高通量(核酸提取) DP337 N96新型植物基因组DNA提取试剂盒(离心板型)(N96 DNAsecure Plant Kit) 2 Plates 1,900.00
96高通量(核酸提取) DP338 N96植物基因组DNA提取试剂盒(离心板型)(N96 Plant Genomic DNA Kit) 2 Plates 1,760.00
96高通量(核酸提取) DP434 N96病毒RNA提取试剂盒(离心板型)(TIANamp N96 Virus RNA Kit) 1板装 3,300.00
DNA纯化试剂盒 DP203-02 离心柱型超薄DNA产物纯化试剂盒(TIANquick Mini Purification Kit) 50 次 240.00
DNA纯化试剂盒 DP204-01 离心柱型普通DNA产物纯化试剂盒(供VT401/402使用)(TIANquick Midi Purification Kit)
20次 150.00
DNA纯化试剂盒 DP204-02 离心柱型普通DNA产物纯化试剂盒(TIANquick Midi Purification Kit) 50 次 240.00
DNA纯化试剂盒 DP204-03 离心柱型普通DNA产物纯化试剂盒(TIANquick Midi Purification Kit) 200 次 860.00
DNA纯化试剂盒 DP205-02 离心柱型大量DNA产物纯化试剂盒(TIANquick Maxi Purification Kit) 50 次 300.00
DNA纯化试剂盒 DP208-02 离心柱型 超薄 琼脂糖凝胶DNA回收试剂盒(TIANgel Mini Purification Kit) 50 次 270.00
DNA纯化试剂盒 DP209-02 离心柱型 普通 琼脂糖凝胶DNA回收试剂盒(TIANgel Midi Purification Kit) 50 次 270.00
DNA纯化试剂盒 DP209-03 离心柱型 普通 琼脂糖凝胶DNA回收试剂盒(TIANgel Midi Purification Kit) 200 次 980.00
DNA纯化试剂盒 DP210-02 离心柱型 大量 琼脂糖凝胶DNA回收试剂盒(TIANgel Maxi Purification Kit) 50 次 300.00
DNA纯化试剂盒 DP212-01 离心柱型寡核苷酸纯化试剂盒(TIANquick Oligo Purification Kit) 20 次 200.00
DNA纯化试剂盒 DP214-02 通用型DNA纯化回收试剂盒(Universal DNA Purification Kit) 50 次 380.00
DNA纯化试剂盒 DP214-03 通用型DNA纯化回收试剂盒(Universal DNA Purification Kit) 200 次 1,180.00
DNA纯化试剂盒 DP215-02 DNA重亚硫酸盐转化试剂盒(离心柱型)DNA Bisulfite Conversion Kit 50preps 1,980.00
DNA纯化试剂盒 RK106-01 结合液PB(Buffer PB) 30 ml 70.00
DNA纯化试剂盒 RK106-02 结合液PB(Buffer PB) 60 ml 130.00
DNA纯化试剂盒 RK106-03 结合液PB(Buffer PB) 120 ml 250.00
DNA纯化试剂盒 RK107 结合液PS(Buffer PS) 30 ml 100.00
DNA纯化试剂盒 RK108-02 溶胶液PN(Buffer PN) 100 ml 180.00
DNA纯化试剂盒 RK110-01 溶液PC(Buffer PC) 25 ml 100.00
SBO物料类别 目录号 物料描述 规格 目录价
DNA纯化试剂盒 RK110-02 溶液PC(Buffer PC) 100 ml 350.00
dNTPs CD111-35 Super Pure dUTP(100mM) 500μ l 800.00
Other Reagent RK122-01 去蛋白液RD(Buffer RD) 12ml 100.00
Other Reagent RK124 RNase-Free 过滤柱CS(RNase-Free Filtration Columns CS) 个 3.00
Other Reagent RK124 RNase-free 过滤柱CS 1套 150.00
Other Reagent RK125-A 吸附柱CA1(Spin Columns CA1) 个 3.00
Other Reagent RK125-A 吸附柱CA1 1套 150.00
Other Reagent RK126-A 吸附柱CA2(Spin Columns CA2) 个 3.00
Other Reagent RK126-A 吸附柱CA2 1套 150.00
Other Reagent RK126-C 吸附柱CP2(Spin Columns CP2) 个 4.00
Other Reagent RK126-C 吸附柱CP2 1套 200.00
Other Reagent RK127-A 吸附柱CA3(Spin Columns CA3) 个 3.00
Other Reagent RK127-A 吸附柱CA3 1套 150.00
Other Reagent RK127-C 吸附柱CP3(Spin Columns CP3) 个 4.00
Other Reagent RK127-C 吸附柱CP3 1套 200.00
Other Reagent RK128-C 吸附柱CP4(Spin Columns CP4) 个 5.00
Other Reagent RK128-C 吸附柱CP4 1套 250.00
Other Reagent RK129-C 吸附柱CP5(Spin Columns CP5) 1个 25.00
Other Reagent RK130 吸附柱CR1(RNase-Free Spin Columns CR1) 个 5.00
Other Reagent RK130 吸附柱CR1 1套 250.00
Other Reagent RK130-C 吸附柱CP6(Spin Columns CB6) 1个 25.00
Other Reagent RK131 RNase-Free 吸附柱CR2(RNase-Free Spin Columns CR2) 个 5.00
Other Reagent RK131 RNase-free 吸附柱CR2 1套 250.00
Other Reagent RK132 RNase-Free 吸附柱CR3(RNase-Free Spin Columns CR3) 个 5.00
Other Reagent RK132 RNase-free 吸附柱CR3 1套 250.00
Other Reagent RK135 过滤器CS(Filtration CS) 1支 20.00
Other Reagent RK137 吸附柱miRspin + 吸附柱miRelute 50套/包 2,000.00
Other Reagent RK143 裂解液RL(Buffer RL) 30ml 280.00
Other Reagent RK155-01 缓冲液LP1(Buffer LP1) 25ml 60.00
Other Reagent RK155-02 缓冲液LP1(Buffer LP1) 100ml 200.00
Other Reagent RK156-01 缓冲液LP2(Buffer LP2) 10ml 30.00
Other Reagent RK156-02 缓冲液LP2(Buffer LP2) 40ml 100.00
SBO物料类别 目录号 物料描述 规格 目录价
Other Reagent RK158-01 缓冲液FP1(Buffer FP1) 25ml 60.00
Other Reagent RK158-02 缓冲液FP1(Buffer FP1) 100ml 200.00
Other Reagent RK159-01 缓冲液FP2(Buffer FP2) 10ml 30.00
Other Reagent RK159-02 缓冲液FP2(Buffer FP2) 40ml 100.00
Other Reagent RK162 去蛋白液MRD(Buffer MRD) 12ml 200.00
Other Reagent RK165 液化柱CX1(Liquefaction Columns CX1) 50个/套 150.00
Other Reagent RK166 液化柱CX2(Liquefaction Columns CX2) 10个/套 80.00
Other Reagent RK167 液化柱CX3(Liquefaction Columns CX3) 10个/套 120.00
Other Reagent RT101 琼脂糖(Agarose(low EEO)) 50 g 250.00
Other Reagent RT103 DTT 二硫苏糖醇 溶液(无菌)(DTT Solution(Sterile)) 5ml 108.00
Other Reagent RT108-01 IPTG 异丙基-β -D-硫代半乳糖苷 溶液(无菌)(IPTG(Sterile)) 5ml 80.00
Other Reagent RT119 X-Gal 溶液 (X-Gal Reagent 20mg/ml) 5 ml 176.00
Other Reagent RT120-01 Deionized Water 100 ml 30.00
Other Reagent RT120-02 Deionized Water 500 ml 70.00
Other Reagent RT120-03 Deionized Water 5×1ml 15.00
Other Reagent RT121-01 DNase/RNase-Free Deionized Water 5×5 ml 35.00
Other Reagent RT121-02 DNase/RNase-Free Deionized Water 100 ml 90.00
Other Reagent RT122-01 红细胞裂解液( Red Cell Lysis Buffer) 100 ml 50.00
Other Reagent RT122-02 红细胞裂解液( Red Cell Lysis Buffer) 250 ml 100.00
Other Reagent RT124-02 加A反应液(Tailing-A Reaction Buffer) 200ul 45.00
Other Reagent RT201-01 6×DNA Loading Buffer(Bromophenol bule) 5 ml 35.00
Other Reagent RT201-02 6×DNA Loading Buffer(Bromophenol bule and Xylene cyanol) 5 ml 40.00
Other Reagent RT202 5×RNA Loading Buffer 5ml 50.00
Other Reagent RT203 Ethidium Bromide(EB)(10mg/ml) 1 ml 30.00
Other Reagent RT204 50×TAE Buffer 50 ml 60.00
Other Reagent RT204 50×TAE Buffer 50ml 60.00
Other Reagent RT205 5×TBE Buffer 500 ml 98.00
Other Reagent RT205 5×TBE Buffer 500ml 98.00
Other Reagent RT209 2×蛋白质电泳Loading Buffer(2×Protein Loading Buffer) 5 ml 50.00
Other Reagent RT210 GeneGreen核酸染料(GeneGreen Nucleic Acid Dye) 500µl 550.00
Other Reagent RT401 溶菌酶溶液(50mg/ml)(Lysozyme ) 1 ml 30.00
Other Reagent RT403-01 Proteinase K (20mg/ml) 500 ul 60.00
SBO物料类别 目录号 物料描述 规格 目录价
Other Reagent RT403-02 Proteinase K (20mg/ml) 5 ml 500.00
Other Reagent RT405-01 RNaseA(10mg/ml) 200 ul 30.00
Other Reagent RT405-02 RNaseA(10mg/ml) 1 ml 80.00
Other Reagent RT405-12 RNaseA(100mg/ml) 500µl 400.00
Other Reagent RT406 T4 DNA Ligase (3U/μ l) 60 U 80.00
Other Reagent RT410-02 Lyticase(10U/ul) 3000 U 240.00
Other Reagent RT411 RNase-Free DNase I 50次 800.00
Other Reagent RT416-02 Carrier RNA 310ug 700.00
Other Reagent RT501 氨苄青霉素钠盐(100mg/ml)(Ampicillin Sodium(100mg/ml)) 5 ml 48.00
Other Reagent RT502 氯霉素(34mg/ml)(Chloramphenicol(34mg/ml)) 5ml 48.00
Other Reagent RT503 硫酸卡那霉素(10mg/ml)(Kanamycin Sulfate(10mg/ml)) 5ml 25.00
Other Reagent RT504 四环素(5mg/ml)(Tetracycline(5mg/ml)) 5 ml 25.00
Other Reagent WHC160 封口膜(Plate Cover) 10张/包 20.00
Other Reagent WP041 透气膜(Permeation) 100张/包 100.00
PCR Mastermix KP201-01 2×Pfu PCR MasterMix(含染料) 1 ml 180.00
PCR Mastermix KP201-02 2×Pfu PCR MasterMix(含染料) 5× 1ml 850.00
PCR Mastermix KP201-11 2×Pfu PCR MasterMix(不含染料) 1 ml 180.00
PCR Mastermix KP201-12 2×Pfu PCR MasterMix(不含染料) 5× 1ml 850.00
PCR Mastermix KP202-01 Fast HiFidelity PCR Kit 50 rxns 300.00
PCR Mastermix KP202-02 Fast HiFidelity PCR Kit 200 rxns 1,000.00
PCR Mastermix KP202-03 Fast HiFidelity PCR Kit 1000 rxns 4,500.00
PCR Mastermix KT109 Taq酶(多重PCR扩增试剂) 50次 1,200.00
PCR Mastermix KT109-01 多重PCR扩增试剂盒(Multi PCR Kit) 50次 1,200.00
PCR Mastermix KT109-02 多重PCR扩增试剂盒(Multi PCR Kit) 100次 2,300.00
PCR Mastermix KT201-01 2 ×Taq PCR MasterMix(含染料) 1 ml 150.00
PCR Mastermix KT201-02 2 ×Taq PCR MasterMix(含染料) 5×1ml 600.00
PCR Mastermix KT201-11 2 ×Taq PCR MasterMix(不含染料) 1 ml 150.00
PCR Mastermix KT201-12 2 ×Taq PCR MasterMix(不含染料) 5×1ml 600.00
PCR Mastermix KT202-01 2×HotStart Taq PCR MasterMix(含染料) 1 ml 270.00
PCR Mastermix KT202-02 2×HotStart Taq PCR MasterMix(含染料) 5× 1ml 1,250.00
PCR Mastermix KT202-11 2×HotStart Taq PCR Master Mix(不含染料) 1 ml 270.00
PCR Mastermix KT202-12 2×HotStart Taq PCR Master Mix(不含染料) 5×1ml 1,250.00
SBO物料类别 目录号 物料描述 规格 目录价
PCR Mastermix KT203-01 2×Long Taq PCR MasterMix(含染料) 1 ml 180.00
PCR Mastermix KT203-02 2×Long Taq PCR MasterMix(含染料) 5× 1ml 850.00
PCR Mastermix KT203-11 2×Long Taq PCR MasterMix(不含) 1 ml 180.00
PCR Mastermix KT203-12 2×Long Taq PCR MasterMix(不含) 5× 1ml 850.00
PCR Mastermix KT204-01 2×Taq Platinum PCR MasterMix(含染料) 1 ml 270.00
PCR Mastermix KT204-02 2×Taq Platinum PCR MasterMix(含染料) 5× 1ml 1,250.00
PCR Mastermix KT204-11 2×Taq Platinum PCR Master Mix(不含染料) 1 ml 270.00
PCR Mastermix KT204-12 2×Taq Platinum PCR Master Mix(不含染料) 5× 1ml 1,250.00
PCR Mastermix KT205-01 2×Taq Plus PCR MasterMix(含染料) 1 ml 180.00
PCR Mastermix KT205-02 2×Taq Plus PCR MasterMix(含染料) 5× 1ml 850.00
PCR Mastermix KT205-11 2×Taq Plus PCR MasterMix(不含) 1 ml 180.00
PCR Mastermix KT205-12 2×Taq Plus PCR MasterMix(不含) 5× 1ml 850.00
PCR Mastermix KT206-01 2xGC-rich PCR marsterMix(含染料) 0.5ml 150.00
PCR Mastermix KT206-11 2xGC-rich PCR Marstermix(不含) 0.5ml 150.00
PCR Mastermix KT207-01 2 X PCR Reagent 1ml 280.00
PCR Mastermix KT208-01 2×Hotmaster PCR MasterMix(含染料) 1 ml 270.00
PCR Mastermix KT208-02 2×hotmaster PCR MasterMix(含染料) 5×1ml 1,250.00
PCR Mastermix KT208-11 2×hotmaster PCR MasterMix(不含) 1ml 270.00
PCR Mastermix KT208-12 2×hotmaster PCR MasterMix(不含) 5×1ml 1,250.00
PCR Mastermix KT221-01 Golden Easy PCR System (含染料) 5 ml 250.00
PCR Mastermix KT221-02 Golden Easy PCR System (含染料) 10 ml 400.00
PCR Mastermix KT501-01 Golden Fast PCR Kit 1ml 180.00
PCR Mastermix KT501-02 Golden Fast PCR Kit 5ml 800.00
PCR Mastermix KT501-11 Golden Fast PCR Kit 100 U 180.00
PCR Mastermix KT501-12 Golden Fast PCR Kit 500 U 800.00
PCR Reagent CD102 T7 Primer(10uM) 200 ul 30.00
PCR Reagent CD104 SP6 Primer (10μ M) 200 ul 30.00
PCR Reagent CD105 Random Hexamers(10uM) 200 ul 30.00
PCR Reagent CD106 Oligo (dT)15(10μ g) 200 ul 30.00
PCR Reagent CD111-02 Super Pure dNTPs(2.5mM each) 1ml 80.00
PCR Reagent CD111-03 Super Pure dNTPs(2.5mM each) 5x1ml 360.00
PCR Reagent CD111-11 Super Pure dNTPs(10mM each) 0.5ml 160.00
SBO物料类别 目录号 物料描述 规格 目录价
PCR Reagent CD111-12 Super Pure dNTPs(10mM each) 1ml 300.00
PCR Reagent CD111-13 Super Pure dNTPs(10mM each) 5x1ml 1,350.00
PCR Reagent CD111-31 Super Pure dATP(100mM each) 0.5ml 350.00
PCR Reagent CD111-32 Super Pure dGTP(100mM each) 0.5ml 350.00
PCR Reagent CD111-33 Super Pure dCTP(100mM each) 0.5ml 350.00
PCR Reagent CD111-34 Super Pure dTTP(100mM each) 0.5ml 350.00
PCR Reagent CD117-01 dNTPs(2.5mM each) 1 ml 50.00
PCR Reagent CD117-02 dNTPs(2.5mM each) 5×1ml 220.00
PCR Reagent CD117-11 dNTPs(10mM each) 1 ml 180.00
PCR Reagent CD117-12 dNTPs(10mM each) 5×1ml 800.00
PCR Reagent CD201-0001 5s 1OD 260.00
PCR Reagent CD201-0002 hsa-let-7e 1OD 260.00
PCR Reagent CD201-0003 hsa-miR-1 1OD 260.00
PCR Reagent CD201-0004 hsa-miR-100-5p 1OD 260.00
PCR Reagent CD201-0005 hsa-miR-106a-5p 1OD 260.00
PCR Reagent CD201-0006 hsa-miR-122-5p 1OD 260.00
PCR Reagent CD201-0007 hsa-miR-124-3p 1OD 260.00
PCR Reagent CD201-0008 hsa-miR-125a-5p 1OD 260.00
PCR Reagent CD201-0009 hsa-miR-128 1OD 260.00
PCR Reagent CD201-0010 hsa-miR-139-5p 1OD 260.00
PCR Reagent CD201-0011 hsa-miR-144-3p 1OD 260.00
PCR Reagent CD201-0012 hsa-miR-145-5p 1OD 260.00
PCR Reagent CD201-0013 hsa-miR-146a-5p 1OD 260.00
PCR Reagent CD201-0014 hsa-miR-150-5p 1OD 260.00
PCR Reagent CD201-0015 hsa-miR-153 1OD 260.00
PCR Reagent CD201-0016 hsa-miR-17-5p 1OD 260.00
PCR Reagent CD201-0017 hsa-miR-17-3p 1OD 260.00
PCR Reagent CD201-0018 hsa-miR-18a-5p 1OD 260.00
PCR Reagent CD201-0019 hsa-miR-191-5p 1OD 260.00
PCR Reagent CD201-0020 hsa-miR-194-5p 1OD 260.00
PCR Reagent CD201-0021 hsa-miR-19a-3p 1OD 260.00
PCR Reagent CD201-0022 hsa-miR-200a-3p 1OD 260.00
SBO物料类别 目录号 物料描述 规格 目录价
PCR Reagent CD201-0023 hsa-miR-200c-3p 1OD 260.00
PCR Reagent CD201-0024 hsa-miR-205-5p 1OD 260.00
PCR Reagent CD201-0025 hsa-miR-27a-3p 1OD 260.00
PCR Reagent CD201-0026 hsa-miR-28-3p 1OD 260.00
PCR Reagent CD201-0027 hsa-miR-29a-3p 1OD 260.00
PCR Reagent CD201-0028 hsa-miR-29b-3p 1OD 260.00
PCR Reagent CD201-0029 hsa-miR-301a-3p 1OD 260.00
PCR Reagent CD201-0031 hsa-miR-301b 1OD 260.00
PCR Reagent CD201-0032 hsa-miR-30b-3p 1OD 260.00
PCR Reagent CD201-0033 hsa-miR-31-5p 1OD 260.00
PCR Reagent CD201-0034 hsa-miR-34a-5p 1OD 260.00
PCR Reagent CD201-0035 hsa-miR-362-3p 1OD 260.00
PCR Reagent CD201-0036 hsa-miR-377-3p 1OD 260.00
PCR Reagent CD201-0038 hsa-miR-425-5p 1OD 260.00
PCR Reagent CD201-0039 hsa-miR-559 1OD 260.00
PCR Reagent CD201-0040 hsa-miR-92a-3p 1OD 260.00
PCR Reagent CD201-0041 hsa-miR-93-5p 1OD 260.00
PCR Reagent CD201-0042 hsa-miR-96-5p 1OD 260.00
PCR Reagent CD201-0043 hsa-miR-99b-5p 1OD 260.00
PCR Reagent CD201-0044 hsa-miR-574-5p 1OD 260.00
PCR Reagent CD201-0045 hsa-miR-297 1OD 260.00
PCR Reagent CD201-0046 hsa-miR-619 1OD 260.00
PCR Reagent CD201-0047 hsa-miR-125b-5p 1OD 260.00
PCR Reagent CD201-0048 hsa-miR-105-5p 1OD 260.00
PCR Reagent CD201-0049 hsa-miR-106a-3p 1OD 260.00
PCR Reagent CD201-0050 hsa-miR-1207-3p 1OD 260.00
PCR Reagent CD201-0051 hsa-miR-1208 1OD 260.00
PCR Reagent CD201-0052 hsa-miR-122-3p 1OD 260.00
PCR Reagent CD201-0053 hsa-miR-1244 1OD 260.00
PCR Reagent CD201-0054 hsa-miR-1253 1OD 260.00
PCR Reagent CD201-0055 hsa-miR-1257 1OD 260.00
PCR Reagent CD201-0056 hsa-miR-1258 1OD 260.00
SBO物料类别 目录号 物料描述 规格 目录价
PCR Reagent CD201-0057 hsa-miR-125b-2-3p 1OD 260.00
PCR Reagent CD201-0058 hsa-miR-126-3p 1OD 260.00
PCR Reagent CD201-0059 hsa-miR-126-5p 1OD 260.00
PCR Reagent CD201-0060 hsa-miR-132-3p 1OD 260.00
PCR Reagent CD201-0061 hsa-miR-137 1OD 260.00
PCR Reagent CD201-0062 hsa-miR-138-5p 1OD 260.00
PCR Reagent CD201-0063 hsa-miR-140-3p 1OD 260.00
PCR Reagent CD201-0064 hsa-miR-140-5p 1OD 260.00
PCR Reagent CD201-0065 hsa-miR-142-3p 1OD 260.00
PCR Reagent CD201-0066 hsa-miR-142-5p 1OD 260.00
PCR Reagent CD201-0067 hsa-miR-143-3p 1OD 260.00
PCR Reagent CD201-0068 hsa-miR-143-5p 1OD 260.00
PCR Reagent CD201-0069 hsa-miR-146a-3p 1OD 260.00
PCR Reagent CD201-0070 hsa-miR-146b-5p 1OD 260.00
PCR Reagent CD201-0071 hsa-miR-149-5p 1OD 260.00
PCR Reagent CD201-0072 hsa-miR-151a-5p 1OD 260.00
PCR Reagent CD201-0073 hsa-miR-154-5p 1OD 260.00
PCR Reagent CD201-0074 hsa-miR-155-5p 1OD 260.00
PCR Reagent CD201-0075 hsa-miR-155-3p 1OD 260.00
PCR Reagent CD201-0076 hsa-miR-17-5p 1OD 260.00
PCR Reagent CD201-0077 hsa-miR-182-5p 1OD 260.00
PCR Reagent CD201-0078 hsa-miR-1825 1OD 260.00
PCR Reagent CD201-0079 hsa-miR-185-5p 1OD 260.00
PCR Reagent CD201-0080 hsa-miR-186-5p 1OD 260.00
PCR Reagent CD201-0081 hsa-miR-18a-3p 1OD 260.00
PCR Reagent CD201-0082 hsa-miR-192-5p 1OD 260.00
PCR Reagent CD201-0083 hsa-miR-195-5p 1OD 260.00
PCR Reagent CD201-0084 hsa-miR-197-3p 1OD 260.00
PCR Reagent CD201-0085 hsa-miR-198 1OD 260.00
PCR Reagent CD201-0086 hsa-miR-19a-5p 1OD 260.00
PCR Reagent CD201-0087 hsa-miR-204-5p 1OD 260.00
PCR Reagent CD201-0088 hsa-miR-20a-5p 1OD 260.00
SBO物料类别 目录号 物料描述 规格 目录价
PCR Reagent CD201-0089 hsa-miR-20a-3p 1OD 260.00
PCR Reagent CD201-0090 hsa-miR-20b 1OD 260.00
PCR Reagent CD201-0091 hsa-miR-20b-3p 1OD 260.00
PCR Reagent CD201-0092 hsa-miR-21-5p 1OD 260.00
PCR Reagent CD201-0093 hsa-miR-21-3p 1OD 260.00
PCR Reagent CD201-0094 hsa-miR-211-5p 1OD 260.00
PCR Reagent CD201-0095 hsa-miR-212-3p 1OD 260.00
PCR Reagent CD201-0096 hsa-miR-217 1OD 260.00
PCR Reagent CD201-0097 hsa-miR-221-3p 1OD 260.00
PCR Reagent CD201-0098 hsa-miR-222-3p 1OD 260.00
PCR Reagent CD201-0099 hsa-miR-223-3p 1OD 260.00
PCR Reagent CD201-0100 hsa-miR-28-5p 1OD 260.00
PCR Reagent CD201-0101 hsa-miR-326 1OD 260.00
PCR Reagent CD201-0102 hsa-miR-340-5p 1OD 260.00
PCR Reagent CD201-0103 hsa-miR-345-5p 1OD 260.00
PCR Reagent CD201-0104 hsa-miR-361-3p 1OD 260.00
PCR Reagent CD201-0105 hsa-miR-361-5p 1OD 260.00
PCR Reagent CD201-0106 hsa-miR-363-3p 1OD 260.00
PCR Reagent CD201-0107 hsa-miR-370 1OD 260.00
PCR Reagent CD201-0108 hsa-miR-371-3p 1OD 260.00
PCR Reagent CD201-0109 hsa-miR-371-5p 1OD 260.00
PCR Reagent CD201-0110 hsa-miR-372 1OD 260.00
PCR Reagent CD201-0111 hsa-miR-373-3p 1OD 260.00
PCR Reagent CD201-0112 hsa-miR-374a-3p 1OD 260.00
PCR Reagent CD201-0113 hsa-miR-374b-3p 1OD 260.00
PCR Reagent CD201-0114 hsa-miR-378-3p 1OD 260.00
PCR Reagent CD201-0115 hsa-miR-379-3p 1OD 260.00
PCR Reagent CD201-0116 hsa-miR-380-3p 1OD 260.00
PCR Reagent CD201-0117 hsa-miR-412 1OD 260.00
PCR Reagent CD201-0118 hsa-miR-422a 1OD 260.00
PCR Reagent CD201-0119 hsa-miR-424-5p 1OD 260.00
PCR Reagent CD201-0120 hsa-miR-450b-3p 1OD 260.00
SBO物料类别 目录号 物料描述 规格 目录价
PCR Reagent CD201-0121 hsa-miR-450b-5p 1OD 260.00
PCR Reagent CD201-0122 hsa-miR-451a 1OD 260.00
PCR Reagent CD201-0123 hsa-miR-488-3p 1OD 260.00
PCR Reagent CD201-0124 hsa-miR-499-5p 1OD 260.00
PCR Reagent CD201-0125 hsa-miR-585 1OD 260.00
PCR Reagent CD201-0126 hsa-miR-589-5p 1OD 260.00
PCR Reagent CD201-0127 hsa-miR-595 1OD 260.00
PCR Reagent CD201-0128 hsa-miR-605 1OD 260.00
PCR Reagent CD201-0129 hsa-miR-609 1OD 260.00
PCR Reagent CD201-0130 hsa-miR-621 1OD 260.00
PCR Reagent CD201-0131 hsa-miR-623 1OD 260.00
PCR Reagent CD201-0132 hsa-miR-624-5p 1OD 260.00
PCR Reagent CD201-0133 hsa-miR-626 1OD 260.00
PCR Reagent CD201-0134 hsa-miR-629-5p 1OD 260.00
PCR Reagent CD201-0135 hsa-miR-629-3p 1OD 260.00
PCR Reagent CD201-0136 hsa-miR-635 1OD 260.00
PCR Reagent CD201-0137 hsa-miR-646 1OD 260.00
PCR Reagent CD201-0138 hsa-miR-650 1OD 260.00
PCR Reagent CD201-0139 hsa-miR-655 1OD 260.00
PCR Reagent CD201-0140 hsa-miR-658 1OD 260.00
PCR Reagent CD201-0141 hsa-miR-7-5p 1OD 260.00
PCR Reagent CD201-0142 hsa-miR-9-5p 1OD 260.00
PCR Reagent CD201-0143 hsa-miR-95 1OD 260.00
PCR Reagent CD201-0144 hsa-miR-98 1OD 260.00
PCR Reagent CD201-0145 hsa-U6 1OD 260.00
PCR Reagent CD201-0146 hsa-miR-101-3p 1OD 260.00
PCR Reagent CD201-0147 hsa-miR-1178 1OD 260.00
PCR Reagent CD201-0148 hsa-miR-1179 1OD 260.00
PCR Reagent CD201-0149 hsa-miR-1183 1OD 260.00
PCR Reagent CD201-0150 hsa-miR-1204 1OD 260.00
PCR Reagent CD201-0151 hsa-miR-1205 1OD 260.00
PCR Reagent CD201-0152 hsa-miR-1224-5p 1OD 260.00
SBO物料类别 目录号 物料描述 规格 目录价
PCR Reagent CD201-0153 hsa-miR-1226-3p 1OD 260.00
PCR Reagent CD201-0154 hsa-miR-1227 1OD 260.00
PCR Reagent CD201-0155 hsa-miR-1236 1OD 260.00
PCR Reagent CD201-0156 hsa-miR-1250 1OD 260.00
PCR Reagent CD201-0157 hsa-miR-136-5p 1OD 260.00
PCR Reagent CD201-0158 hsa-miR-144-5p 1OD 260.00
PCR Reagent CD201-0159 hsa-miR-1468 1OD 260.00
PCR Reagent CD201-0160 hsa-miR-146b-3p 1OD 260.00
PCR Reagent CD201-0161 hsa-miR-151-3p 1OD 260.00
PCR Reagent CD201-0162 hsa-miR-206 1OD 260.00
PCR Reagent CD201-0163 hsa-miR-215 1OD 260.00
PCR Reagent CD201-0164 hsa-miR-219-1-3p 1OD 260.00
PCR Reagent CD201-0165 hsa-miR-32-5p 1OD 260.00
PCR Reagent CD201-0166 hsa-miR-335-5p 1OD 260.00
PCR Reagent CD201-0167 hsa-miR-337-5p 1OD 260.00
PCR Reagent CD201-0168 hsa-miR-342-5p 1OD 260.00
PCR Reagent CD201-0169 hsa-miR-346 1OD 260.00
PCR Reagent CD201-0170 hsa-miR-34b-5p 1OD 260.00
PCR Reagent CD201-0171 hsa-miR-34c-5p 1OD 260.00
PCR Reagent CD201-0172 hsa-miR-367-3p 1OD 260.00
PCR Reagent CD201-0173 hsa-miR-375 1OD 260.00
PCR Reagent CD201-0174 hsa-miR-377-5p 1OD 260.00
PCR Reagent CD201-0175 hsa-miR-379-5p 1OD 260.00
PCR Reagent CD201-0176 hsa-miR-381 1OD 260.00
PCR Reagent CD201-0177 hsa-miR-382-5p 1OD 260.00
PCR Reagent CD201-0178 hsa-miR-383 1OD 260.00
PCR Reagent CD201-0179 hsa-miR-384 1OD 260.00
PCR Reagent CD201-0180 hsa-miR-432-5p 1OD 260.00
PCR Reagent CD201-0181 hsa-miR-483-5p 1OD 260.00
PCR Reagent CD201-0182 hsa-miR-486-5p 1OD 260.00
PCR Reagent CD201-0183 hsa-miR-606 1OD 260.00
PCR Reagent CD201-0184 hsa-miR-610 1OD 260.00
SBO物料类别 目录号 物料描述 规格 目录价
PCR Reagent CD201-0185 hsa-miR-622 1OD 260.00
PCR Reagent CD201-0186 hsa-miR-634 1OD 260.00
PCR Reagent CD201-0187 hsa-miR-657 1OD 260.00
PCR Reagent CD202-0001 mmu-miR-10a-5p 1OD 260.00
PCR Reagent CD202-0002 mmu-miR-10 1OD 260.00
PCR Reagent CD202-0003 mmu-miR-107-3p 1OD 260.00
PCR Reagent CD202-0004 mmu-miR-10b-5p 1OD 260.00
PCR Reagent CD202-0005 mmu-miR-129-3p 1OD 260.00
PCR Reagent CD202-0006 mmu-miR-133a-3p 1OD 260.00
PCR Reagent CD202-0007 mmu-miR-16-5p 1OD 260.00
PCR Reagent CD202-0008 mmu-miR-194-5p 1OD 260.00
PCR Reagent CD202-0009 mmu-miR-24-3p 1OD 260.00
PCR Reagent CD202-0010 mmu-miR-29a-3p 1OD 260.00
PCR Reagent CD202-0011 mmu-miR-31-5p 1OD 260.00
PCR Reagent EM101-01 Methylation-specific PCR(MSP)Kit 甲基化特异性PCR试剂盒 400U 680.00
PCR Reagent EP101-01-01 Pfu DNA Polymerase 250U(2.5u/ul含) 150.00
PCR Reagent EP101-02-01 Pfu DNA Polymerase 500U (2.5U/ul含) 280.00
PCR Reagent ET101-01-01 Taq DNA Polymerase 250U(2.5U/ul含) 95.00
PCR Reagent ET101-01-02 Taq DNA Polymerase 250U(2.5U/ul分) 95.00
PCR Reagent ET101-01-03 Taq DNA Polymerase 250U(5U/ul含) 95.00
PCR Reagent ET101-01-04 Taq DNA Polymerase 250U(5U/ul分) 95.00
PCR Reagent ET101-02-01 Taq DNA Polymerase 500U(2.5U/ul含) 175.00
PCR Reagent ET101-02-02 Taq DNA Polymerase 500U(2.5U/ul分) 175.00
PCR Reagent ET101-02-03 Taq DNA Polymerase 500U(5U/ul含) 175.00
PCR Reagent ET101-02-04 Taq DNA Polymerase 500U(5U/ul分) 175.00
PCR Reagent ET103-01-01 Long Taq DNA Polymerase 250U(2.5U/ul含) 150.00
PCR Reagent ET103-02-01 Long Taq DNA Polymerase 500U(2.5U/ul含) 280.00
PCR Reagent ET104-01-01 Taq Platinum DNA Polymerase 250U(2.5U/ul含) 250.00
PCR Reagent ET104-02-01 Taq Platinum DNA Polymerase 500U(2.5U/ul含) 460.00
PCR Reagent ET105-01-01 Taq Plus DNA Polymerase 250U(2.5U/ul含) 120.00
PCR Reagent ET105-02-01 Taq Plus DNA Polymerase 500U(2.5U/ul含) 220.00
PCR Reagent ET106-01-01 HotMaster Taq DNA Polymerase 250U(2.5U/ul含) 300.00
SBO物料类别 目录号 物料描述 规格 目录价
PCR Reagent ET106-02-01 HotMaster Taq DNA Polymerase 500U(2.5U/ul含) 580.00
PCR Reagent ET107-02-03 SuperPure Taq DNA Polymerase 500U(5U/μ l含) 350.00
PCR Reagent ET107-02-04 SuperPure Taq DNA Polymerase 500U(5U/μ l分) 350.00
PCR Reagent ET108-01 High Affinity HotStart Taq高亲和力抗体修饰的 Taq DNA 聚合酶 250U 450.00
PCR Reagent ET108-02 High Affinity HotStart Taq高亲和力抗体修饰的 Taq DNA 聚合酶 500U 830.00
PCR Reagent ET110 Taq II DNA Polymerase 250U 250.00
PCR Reagent KG203-02 TIANcombi DNA Lyse&Det PCR Kit 快速DNA提取检测试剂盒 50preps 600.00
PCR Reagent KG203-03 TIANcombi DNA Lyse&Det PCR Kit 快速DNA提取检测试剂盒 200preps 1,920.00
PCR Reagent KM101 快速定点突变试剂盒 (Fast Site-Directed Mutagenesis Kit) 20rxn 1,300.00
1800 μ l
PCR Reagent RP101 10×PCR Reaction Buffer I 10.00
1800 μ l
PCR Reagent RP101-04 10×PCR Reaction Buffer(Mg2+ Free) 10.00
1800 μ l
PCR Reagent RP102-01 10×PCR Reaction Buffer II 10.00
PCR Reagent RP102-02 10×Pfu Reation Buffer(Mg2+ Free) 1800 ul 10.00
1800 μ l
PCR Reagent RP107 25mM MgCl2 10.00
PCR Reagent RP202 PCR Enhancer 500ul 90.00
Realtime PCR ET108-T 2×High Affinity HotStart Taq MasterMix (probe qPCR used) 50ml 9,800.00
Realtime PCR FP202-01 RealMasterMix(2.5X) 50 次 500.00
Realtime PCR FP202-02 RealMasterMix(2.5X) 200 次 1,700.00
Realtime PCR FP203-01 荧光定量试剂盒RealMasterMix(Probe) 50 次 500.00
Realtime PCR FP203-02 荧光定量试剂盒RealMasterMix(Probe) 200 次 1,700.00
Realtime PCR FP204-01 SuperReal 荧光定量预混试剂(SuperReal PreMix(SYBR Green)) 50次 580.00
Realtime PCR FP204-02 SuperReal 荧光定量预混试剂(SuperReal PreMix(SYBR Green)) 200次 1,980.00
Realtime PCR fp204-03 SuperReal PreMix(SYBR Green) 2000次 16,800.00
Realtime PCR FP205-01 SuperReal 荧光定量预混试剂 增强版(SYBR Green)(SuperReal PreMix Plus (SYBR rxns
125 Green)) 580.00
Realtime PCR FP205-02 SuperReal 荧光定量预混试剂 增强版(SYBR Green)(SuperReal PreMix Plus (SYBR rxns
500 Green)) 1,980.00
Realtime PCR FP206-01 SuperReal PreMix(Probe) SuperReal 荧光定量预混试剂(探针法) 125rxn 580.00
Realtime PCR FP206-02 SuperReal PreMix(Probe) SuperReal 荧光定量预混试剂(探针法) 500rxn 1,980.00
Realtime PCR FP207-01 FastFire qPCR PreMix (SYBR Green)FastFire快速荧光定量PCR预混试剂(SYBR Green)
125rxn 580.00
Realtime PCR FP207-02 FastFire qPCR PreMix (SYBR Green)FastFire快速荧光定量PCR预混试剂(SYBR Green)
500rxn 1,980.00
Realtime PCR FP207-03 FastFire qPCR PreMix (SYBR Green)FastFire快速荧光定量PCR预混试剂(SYBR Green)
5000rxn 16,800.00
Realtime PCR FP208-01 FastFire qPCR PreMix(Probe)FastFire快速定量PCR试剂 (探针法) 125rxn 580.00
Realtime PCR FP208-02 FastFire qPCR PreMix(Probe)FastFire快速定量PCR试剂 (探针法) 500rxn 1,980.00
SBO物料类别 目录号 物料描述 规格 目录价
Realtime PCR FP210-01 HRM Analysis Kit (EvaGreen) HRM分析试剂盒 (EvaGreen) 125rxn 720.00
Realtime PCR FP210-02 HRM Analysis Kit (EvaGreen) HRM分析试剂盒 (EvaGreen) 500rxn 2,480.00
Realtime PCR FP211-01 TIANtough Genotyping qPCR PreMix(Probe) TIANtough基因分型PCR试剂 (探针法) rxn
125 700.00
Realtime PCR FP211-02 TIANtough Genotyping qPCR PreMix(Probe) TIANtough基因分型PCR试剂 (探针法) rxn
500 2,380.00
Realtime PCR FP302-01 Quant qRT-PCR (SYBR Green)Kit 50次 1,280.00
Realtime PCR fp302-01 Quant qRT-PCR (SYBR Green)Kit 50次 1,280.00
Realtime PCR FP302-02 Quant qRT-PCR (SYBR Green)Kit 200次 3,680.00
Realtime PCR fp302-02 Quant qRT-PCR (SYBR Green)Kit 200次 3,680.00
Realtime PCR FP303 Quant One Step qRT-PCR(SYRR Green I)Kit 试用装 150.00
Realtime PCR FP303-01 Quant One Step qRT-PCR(SYRR Green I)Kit 50次X50ul 1,500.00
Realtime PCR FP304 Quant One Step qRT-PCR(Probe)Kit 试用装 150.00
Realtime PCR FP304-01 Quant One Step qRT-PCR(Probe)Kit 50次 1,500.00
Realtime PCR FP304-02 Quant One Step qRT-PCR(Probe)Kit 200次 4,980.00
Realtime PCR FP401 miRcute miRNA 荧光定量检测试剂盒(miRcute miRNA qPCR Detection Kit(SYBR Green) )
50次 880.00
RNA reagent DP405-01 TRNzol Reagent 20 ml 180.00
RNA reagent DP405-01 TRNzol 总RNA提取试剂(TRNzol Reagent ) 20 ml 180.00
RNA reagent DP405-02 TRNzol Reagent 100 ml 600.00
RNA reagent DP405-02 TRNzol 总RNA提取试剂(TRNzol Reagent ) 100 ml 600.00
RNA reagent DP408-01 RNAstore Reagent 20 ml 120.00
RNA reagent DP408-01 RNAstore样本保存液(RNAstore Reagent ) 20ml 120.00
RNA reagent DP408-02 RNAstore Reagent 100 ml 480.00
RNA reagent DP408-02 RNAstore样本保存液(RNAstore Reagent ) 100ml 480.00
RNA reagent DP409 RNAsafe (RNase 抑制剂)20×(RNAsafe (RNase Inhibitor)) 1 ml 190.00
RNA reagent DP418 Rnasin 30ul/ 支 200.00
RNA reagent DP418 RNasin 30ul/支 370.00
RNA reagent DP421 TRNzolA+ Reagent 100 ml 880.00
RNA reagent DP421 TRNzol-A+总RNA提取试剂(TRNzol-A+ Reagent ) 100ml 880.00
RNA reagent DP421-01 TRNzolA+ Reagent 20 ml 260.00
RNA reagent DP437-01 RNAplant Plus Reagent 16 ml 200.00
RNA reagent DP437-01 植物RNA提取试剂(RNAplant Plus Reagent ) 16ml 200.00
RNA reagent DP437-02 RNAplant Plus Reagent 80 ml 680.00
RNA reagent DP437-02 植物RNA提取试剂(RNAplant Plus Reagent ) 80ml 680.00
SBO物料类别 目录号 物料描述 规格 目录价
RNA提取试剂盒 DP315-R 病毒RNA提取试剂盒(TIANamp Virus RNA Kit ) 50次 960.00
RNA提取试剂盒 DP412 RNA纯化试剂盒(RNAclean Kit) 20 次 360.00
RNA提取试剂盒 dp419 RNAsimple总RNA提取试剂盒 50次 680.00
RNA提取试剂盒 dp420 RNAprep Micro微量样品总RNA提取试剂盒 50次 1,580.00
RNA提取试剂盒 DP422 DNA/RNA共提取试剂盒(DNA/RNA Isolation Kit) 50次 1,400.00
RNA提取试剂盒 DP423 DNA/RNA/蛋白质共提取试剂盒(DNA/RNA/Protein Isolation Kit) 50次 2,800.00
RNA提取试剂盒 dp430 RNAprep pure培养细胞/细菌总RNA提取试剂盒 50次 1,280.00
RNA提取试剂盒 dp431 RNAprep pure动物组织总RNA提取试剂盒 50次 1,380.00
RNA提取试剂盒 dp432 RNAprep pure植物总RNA提取试剂盒 50次 1,280.00
RNA提取试剂盒 dp433 RNAprep pure血液样品总RNA提取试盒 50次 1,380.00
RNA提取试剂盒 dp439 RNAprep pure FFPE Kit 50次 1,680.00
RNA提取试剂盒 DP440-01 RNALock 血液RNA稳定剂(RNALock Reagent) 20 ml 200.00
RNA提取试剂盒 DP440-02 RNALock 血液RNA稳定剂(RNALock Reagent) 100 ml 780.00
RNA提取试剂盒 DP441 RNAprep Pure Plant Kit(Polysaccharides&Polyphenolics-rich)RNAprep Pure多糖多酚植物总RNA提取试剂盒(离心柱型)
50次 1,480.00
RNA提取试剂盒 DP442 RNAprep Pure mRNA Purification Kit RNAprep Pure mRNA纯化试剂盒(离心柱型)
10次 1,380.00
RNA提取试剂盒 dp501 miRcute miRNA 提取分离试剂盒 50次 2,380.00
RNA提取试剂盒 RK118 裂解液RL/RV+/RV(Buffer RL/RV+/RV) 30 ml 280.00
RNA提取试剂盒 RK122-01 去蛋白液RD/MRD (Buffer RD/MRD) 12 ml 100.00
RNA提取试剂盒 RK123 漂洗液RW(Buffer RW) 15 ml 30.00
RNA提取试剂盒 RK138 裂解液Rlplus(Buffer RLplus) 30 ml 600.00
RNA提取试剂盒 RK144 溶液RK(Buffer RK) 10 ml 120.00
RNA提取试剂盒 RK145 裂解液RZ(与DP419配套用)(Buffer RZ) 60ml 420.00
RNA提取试剂盒 RK146 去蛋白液RW1(Buffer RW1) 40 ml 200.00
RNA提取试剂盒 RK148 缓冲液HL(Buffer HL) 30 ml 280.00
RNA提取试剂盒 RK149 裂解液MZ(与DP501配套用)(Buffer MZ) 60ml 800.00
RNA提取试剂盒 RK163 裂解液SL(Buffer SL) 30 ml 380.00
RNA提取试剂盒 RT121 RNase-Free ddH2O 1 ml 10.00
RNA提取试剂盒 RT121-01 DNase/RNase-Free Deionized Water 5 ml 15.00
RNA提取试剂盒 RT121-02 DNase/RNase-Free Deionized Water 100 ml 90.00
RNA提取试剂盒 RT121-03 RNase-Free ddH2O 15 ml 20.00
RNA提取试剂盒 RT121-04 RNase-Free ddH2O 40 ml 50.00
RNA提取试剂盒 SD101 病毒检测用RNA提取试剂盒(TIANamp RNA Kit for Virus detection) 50次 1,680.00
SBO物料类别 目录号 物料描述 规格 目录价
30 μ l
RT-PCR DP418 RNasin(40U/ul) 200.00
RT-PCR ER103-02 Quant Reverse Transcriptase 25次 850.00
RT-PCR ER103-03 Quant Reverse Transcriptase 50次 1,500.00
RT-PCR ER103-04 Quant Reverse Transcriptase 100次 2,800.00
RT-PCR ER104-03 TIANScript M-MLV 5000U 250.00
RT-PCR ER104-04 TIANScript M-MLV 20000U 850.00
RT-PCR KR103-03 Quant cDNA 第一链合成试剂盒(QuantScript RT Kit) 25次 1,000.00
RT-PCR KR103-04 Quant cDNA 第一链合成试剂盒(QuantScript RT Kit) 100次 3,000.00
RT-PCR KR104-01 TIANscript cDNA第一链合成试剂盒(TIANScript RT Kit) 25次 400.00
RT-PCR KR104-02 TIANscript cDNA第一链合成试剂盒(TIANScript RT Kit) 100次 1,380.00
RT-PCR KR105 FastLine Cell cDNA Kit FastLine细胞到cDNA快速制备试剂盒 50次 2,680.00
RT-PCR KR106-01 FastQuant cDNA第一链合成试剂盒(去基因组)(FastQuant RT Kit(with gDNase))
25rxns 660.00
RT-PCR KR106-02 FastQuant cDNA第一链合成试剂盒(去基因组)(FastQuant RT Kit(with gDNase))
100rxns 1,980.00
RT-PCR KR107-01 TIANScriptⅡcDNA第一链合成试剂盒(TIANScriptⅡ RT Kit) 25 rxn 500.00
RT-PCR KR107-02 TIANScriptⅡcDNA第一链合成试剂盒(TIANScriptⅡ RT Kit) 100 rxn 1,680.00
RT-PCR KR113 Quant OneStep RT-PCR Kit-一步法试剂盒(Quant One Step RT-PCR Kit) 50次 1,400.00
RT-PCR KR201-01 miRcute miRNA cDNA第一链合成试剂盒(miRcute miRNA First-Strand cDNA Synthesis Kit)
25次 2,280.00
RT-PCR KR201-02 miRcute miRNA cDNA第一链合成试剂盒(miRcute miRNA First-Strand cDNA Synthesis Kit)
50次 4,180.00
RT-PCR SD102 病毒检测用一步法RT-PCR试剂盒(One-step RT-PCR Kit for Virus Detection) 50次 1,800.00
RT-PCR SD103 增强型 病毒检测用一步法RT-PCR试剂盒(Super Onestep RT-PCR Kit for Virus Detection)
50次 1,980.00
TIANGEN DNA Marker MD101-01 DNA Marker Ⅰ 50 次 90.00
TIANGEN DNA Marker MD101-02 DNA Marker Ⅰ 200 次 300.00
TIANGEN DNA Marker MD102-01 DNA Marker Ⅱ 50 次 90.00
TIANGEN DNA Marker MD102-02 DNA Marker Ⅱ 200 次 300.00
TIANGEN DNA Marker MD103-01 DNA Marker Ⅲ 50 次 90.00
TIANGEN DNA Marker MD103-02 DNA Marker Ⅲ 200 次 300.00
TIANGEN DNA Marker MD104-01 DNA Marker Ⅳ 50 次 90.00
TIANGEN DNA Marker MD104-02 DNA Marker Ⅳ 200 次 300.00
TIANGEN DNA Marker MD105-01 DNA Marker Ⅴ 50 次 90.00
TIANGEN DNA Marker MD105-02 DNA Marker Ⅴ 200 次 300.00
TIANGEN DNA Marker MD107-01 DNA Marker Ⅶ 50 次 90.00
TIANGEN DNA Marker MD107-02 DNA Marker Ⅶ 200 次 300.00
SBO物料类别 目录号 物料描述 规格 目录价
TIANGEN DNA Marker MD108-01 50 bp DNA Ladder 50 次 160.00
TIANGEN DNA Marker MD108-02 50 bp DNA Ladder 200 次 600.00
TIANGEN DNA Marker MD109-01 100 bp DNA Ladder 50 次 160.00
TIANGEN DNA Marker MD109-02 100 bp DNA Ladder 200 次 600.00
TIANGEN DNA Marker MD110-01 D15000 50 次 90.00
TIANGEN DNA Marker MD110-02 D15000 200 次 300.00
TIANGEN DNA Marker MD111-01 1 kb DNA Ladder 50 次 90.00
TIANGEN DNA Marker MD111-02 1 kb DNA Ladder 200 次 300.00
TIANGEN DNA Marker MD112-01 500 bp DNA Ladder 50 次 90.00
TIANGEN DNA Marker MD112-02 500 bp DNA Ladder 200 次 300.00
TIANGEN DNA Marker MD113-01 1 kb Plus DNA Ladder 50 次 160.00
TIANGEN DNA Marker MD113-02 1 kb Plus DNA Ladder 200次 600.00
TIANGEN DNA Marker MD114-01 D2000 50 次 90.00
TIANGEN DNA Marker MD114-02 D2000 200 次 300.00
TIANGEN DNA Marker MD115-01 200bp DNA Ladder 50 次 160.00
TIANGEN DNA Marker MD115-02 200bp DNA Ladder 200 次 600.00
TIANGEN DNA Marker MD116-01 D15000+2000 50 次 160.00
TIANGEN DNA Marker MD116-02 D15000+2000 200 次 600.00
λ
TIANGEN DNA Marker MD202-01 DNA/ HindⅢ 50次 90.00
λ
TIANGEN DNA Marker MD202-02 DNA/ HindⅢ 200次 320.00
TIANGEN DNA Marker MD203-01 pUC18 DNA/MspI 20次 90.00
TIANGEN DNA Marker MD203-02 pUC18 DNA/MspI 100次 380.00
TIANGEN DNA Marker MD206-01 pBR322 DNA/MspI 20次 90.00
TIANGEN DNA Marker MD206-02 pBR322 DNA/MspI 100次 380.00
200 μ l
Vector (载体) CD107 pZero Back/blunt Forward Sequencing Primer(10μ M) 68.00
200 μ l
Vector (载体) CD108 pZero Back/blunt Reverse Sequencing Primer(10μ M) 68.00
Vector (载体) VI102-01 pGM-T重组菌落PCR鉴定试剂盒(PCR Identification Kit For Recombinant pGM-T Clone )
50次 120.00
Vector (载体) VT202-01 pGM-T 连接试剂盒(pGM-T Cloning Kit) 20 ul 390.00
Vector (载体) VT202-02 pGM-T 连接试剂盒(pGM-T Cloning Kit) 60 ul 980.00
Vector (载体) VT204-01 零背景快速连接试剂盒(ZeroBack Fast Ligation Kit) 20ul 900.00
Vector (载体) VT204-03 零背景快速连接试剂盒(ZeroBack Fast Ligation Kit) 10ul 560.00
Vector (载体) vt302-01 pGM-T 产物克隆试剂盒 20ul 500.00
SBO物料类别 目录号 物料描述 规格 目录价
Vector (载体) vt302-02 pGM-T 产物克隆试剂盒 60ul 1,300.00
Vector (载体) vt402 pGM-T平末端加A克隆试剂盒 20ul 650.00
蛋白产品 AB101-01 Anti-GST Antibody 20ul (1mg/ml) 320.00
蛋白产品 AB101-02 Anti-GST Antibody 100ul(1mg/ml) 1,180.00
蛋白产品 AB102-01 Anti-His Antibody 20ul(1mg/ml) 320.00
蛋白产品 AB102-02 Anti-His Antibody 100ul 1,180.00
蛋白产品 AB103-01 Anti-Myc Antibody 20ul(1mg/ml) 320.00
蛋白产品 AB103-02 Anti-Myc Antibody 100μ l (1mg/ml) 1,180.00
蛋白产品 AB104-01 Ani-HA Antibody 20ul(1mg/ml) 320.00
蛋白产品 AB104-02 Anti-HA Antibody 100ul (1mg/ml) 1,180.00
蛋白产品 AB105 Anti-GFP Antibody 5ul试用装 80.00
蛋白产品 AB105-01 Anti-GFP Antibody 20ul (1mg/ml) 320.00
蛋白产品 AB105-02 Anti-GFP Antibody 100ul (1mg/ml) 1,180.00
蛋白产品 MP102 蛋白质分子量标准 14.4KDa – 94.0KDa( Molecular Weight Marker ) 20 次 90.00
蛋白产品 MP203 预染蛋白质MarkerⅡ(蓝色)19 kDa – 117 kDa(Pre-stained Protein Marker Ⅱ (Bule))
20 次 260.00
蛋白产品 MP204 蓝色预染蛋白质MarkerⅢ (蓝色)18 KDa - 94 Kda(Pre-stained Protein Marker 次 (Bule))
20 Ⅲ 180.00
蛋白产品 MP205 双色预染蛋白质 Marker 20次 220.00
蛋白产品 MP206 宽范围彩色预染蛋白质Marker Pre-stained Multicolor Broad Range Protein 20次
Marker 11KDa – 245KDa
350.00
蛋白产品 PA101-01 考马斯亮蓝快速染色液(CBB Fast Staining Solution) 100 ml 50.00
蛋白产品 pa102 Bradford蛋白质定量试剂盒 100次 200.00
蛋白产品 PA106-01 Western Blot 膜封闭液(WB Membrane Blocking Solution) 100 ml 60.00
蛋白产品 PA107-01 可溶型单组分TMB底物溶液(Soluble TMB Substrate Solution) 100 ml 180.00
蛋白产品 pa107-02 可溶型单组分TMB底物溶液 5*100ml 680.00
蛋白产品 PA108-01 沉淀型单组分TMB底物溶液(Sedimentary TMB Substrate Solution) 100 ml 180.00
蛋白产品 pa108-02 沉淀型单组分TMB底物溶液 5x100ml 680.00
蛋白产品 PA109 HRP-DAB 底物显色试剂盒(HRP-DAB Chromogenic Substrate Kit) 60 ml 150.00
蛋白产品 PA110 增强型HRP-DAB底物显色试剂盒(Enhanced HRP-DAB Chromogenic Substrate Kit ) ml
60 170.00
蛋白产品 PA111 BCIP/NBT 底物显色试剂盒(BCIP/NBT Chromogenic Substrate Kit) 60 ml 180.00
蛋白产品 PA112-01 Pro-Light HRP化学发光检测试剂(Pro-light HRP Chemiluminescent Kit ) 25 ml 280.00
蛋白产品 PA112-02 Pro-Light HRP化学发光检测试剂(Pro-light HRP Chemiluminescent Kit ) 50 ml 520.00
蛋白产品 PA114 Western Blot蛋白印迹膜再生液(Western Blot Stripping Buffer) 100ml 150.00
蛋白产品 pa115-01 BCA蛋白质定量试剂盒 50/500次 380.00
SBO物料类别 目录号 物料描述 规格 目录价
蛋白产品 pa115-02 BCA蛋白质定量试剂盒 250/2500次 1,000.00
蛋白产品 SA101-01 羊抗鼠 lgG-HRP 0.1 ml 45.00
蛋白产品 SA101-02 羊抗鼠 lgG-HRP 0.5ml 180.00
感受态细胞 CB101-01 DH5α 10×100ul 160.00
感受态细胞 CB101-02 DH5α 20 × 100ul 290.00
感受态细胞 CB101-03 DH5α 5×100ul 90.00
感受态细胞 CB104-01 TOP10 10×100ul 160.00
感受态细胞 CB104-02 TOP10 20 ×100ul 290.00
感受态细胞 CB104-03 TOP10 5× 100ul 90.00
感受态细胞 CB105-01 BL21(DE3) 5x100ul 200.00
感受态细胞 CB105-02 BL21(DE3) 10×100ul 280.00
感受态细胞 CB106-01 BL21(DE3)Plyss 5x100ul 200.00
感受态细胞 CB106-02 BL21(DE3)Plyss 10x100ul 280.00
感受态细胞 CB107 FDM感受态细胞 (FDM competent cells) 10×50µl 90.00
库存商品 HC101 10ul Tip(袋装,无色) 1000支/包 25.00
库存商品 HC104 200ul Tip(袋装,黄色) 1000个/包 25.00
库存商品 HC107 1000ul Tip(袋装,蓝色) 500个/包 25.00
库存商品 HC110 5000ul Tip(袋装,无色) 100个/包 30.00
库存商品 HC111 0.2ml薄壁离心管(袋装,无色) 1000个/包 45.00
库存商品 HC112 96孔薄壁管板 0 8.00
库存商品 HC116 1.5ml离心管(袋装,无色) 500个/包 25.00
库存商品 HC118 2ml冻存管(袋装,无色,螺口,可立) 200支/包 60.00
库存商品 HC119 5ml冻存管(袋装,无色,螺口,可立,带刻度) 200支/包 100.00
库存商品 HC120 15ml离心管(袋装,无色,尖底,螺口,带刻度) 50支/包 80.00
库存商品 HC121 50ml离心管(袋装,无色,低速尖底螺口,带刻度) 50支/包 90.00
库存商品 HC122 10ul Tip盒(96孔,方形) 0 8.00
库存商品 HC123 200ul Tip盒(96孔,方形) 0 8.00
库存商品 HC124 1000ul Tip盒(60孔,方形) 0 8.00
库存商品 HC125 1.5ml离心管盒(72孔,方形) 0 8.00
库存商品 HC131 双面离心管架(长方形) 0 18.00
库存商品 WL046 研磨杵 50支/包 100.00
库存商品 WL239-A 一次性针头滤器(millipore) 0 15.00
SBO物料类别 目录号 物料描述 规格 目录价
库存商品 WP027-02 96孔深孔板(2.2mlU型底) 0 25.00
库存商品 WQ001 1.5ml本色螺口冻存管(进口)(不含盖) 500个/包 400.00
库存商品 WQ001-02 15ml螺口冻存管(进口) 50个/包 350.00
库存商品 WQ001-03 50ml螺口冻存管(进口) 50个/包 400.00
库存商品 WQ001-04 5ml螺口冻存管(进口) 100个/包 300.00
库存商品 WQ002-1 蓝色螺口盖(进口) 500个/包 400.00
库存商品 WQ002-2 红色螺口盖(进口) 500个/包 400.00
库存商品 WQ002-3 绿色螺口盖(进口) 500个/包 400.00
库存商品 WQ002-4 白色螺口盖(进口) 500个/包 400.00
库存商品 WQ002-5 透明螺口盖(进口) 500个/包 400.00
库存商品 WQ002-6 黄色螺口盖(进口) 500个/包 400.00
库存商品 WQ028-01 0.5ml棕色可立螺口冻存管(进口、含盖) 500个/包 700.00
库存商品 WQ028-02 2ml棕色可立螺口冻存管(进口、含盖) 500个/包 700.00
库存商品(5prime) WM5-2302800 Phase Lock Gel Light 1.5ml 200次 1,070.00
库存商品(5prime) WM5-2302810 Phase Lock Gel Heavy 1.5ml 200个 1,070.00
库存商品(5prime) WM5-2302820 Phase Lock Gel Light 2ml 200次 1,070.00
库存商品(5prime) WM5-2302830 Phase Lock Gel Heavy 2ml 200次 1,070.00
库存商品(5prime) WM5-2302830-T Phase lock Gel heavy 2ml 5个/包 50.00
库存商品(5prime) WM5-2302831 Phase Lock Gel Heavy 2ml 100个 623.00
库存商品(5prime) WM5-2302840 Phase Lock Gel Light 15ml 100次 890.00
库存商品(5prime) WM5-2302850 Phase Lock Gel Heavy 15ml 100次 890.00
库存商品(5prime) WM5-2302860 Phase Lock Gel Light 50ml 25次 720.00
库存商品(5prime) WM5-2302870 Phase Lock Gel Heavy 50ml 25次 720.00
库存商品(Bioworks) WM6-17-100-102 WorkBeads 17 Q 4.3ml 17,469.00
库存商品(Bioworks) WM6-17-200-102 WorkBeads 17 S 4.3ml 17,469.00
库存商品(Bioworks) WM6-17-300-101 WorkBeads 17 SEC 15ml 11,454.00
库存商品(Bioworks) WM6-17-300-102 WorkBeads 17 SEC 25ml 13,431.00
库存商品(Bioworks) WM6-17-350-102 WorkBeads 17/100 SEC,25ml pre-packed column 25ml 13,431.00
库存商品(Bioworks) WM6-40-100-001 WorkBeads 40 Q 25ml 948.00
库存商品(Bioworks) WM6-40-100-001S WorkBeads 40 Q 25ML 920.00
库存商品(Bioworks) WM6-40-100-002 WorkBeads 40 Q 200ml 2,966.00
库存商品(Bioworks) WM6-40-100-010 WorkBeads 40 Q 1L 9,806.00
SBO物料类别 目录号 物料描述 规格 目录价
库存商品(Bioworks) WM6-40-100-102 WorkBeads 40 Q 4.3ml 6,584.00
库存商品(Bioworks) WM6-40-100-102S WorkBeads 40 Q 4.3ML 6,392.00
库存商品(Bioworks) WM6-40-150-001 WorksBeads 40 DEAE 25ml 808.00
库存商品(Bioworks) WM6-40-150-001S WorkBeads 40 DEAE 25ML 784.00
库存商品(Bioworks) WM6-40-200-001 WorkBeads 40 S 25ml 948.00
库存商品(Bioworks) WM6-40-200-001S WorkBeads 40 S 25ML 920.00
库存商品(Bioworks) WM6-40-200-002 WorkBeads 40 S 200ml 2,966.00
库存商品(Bioworks) WM6-40-200-010 WorkBeads 40 S 1L 9,806.00
库存商品(Bioworks) WM6-40-200-102 WorkBeads 40 S 4.3ml 6,584.00
库存商品(Bioworks) WM6-40-200-102S WorkBeads 40 S 4.3ML 6,392.00
库存商品(Bioworks) WM6-40-300-001 WorkBeads 40 SEC 25ml 808.00
库存商品(Bioworks) WM6-40-300-001S WorkBeads 40 SEC 25ML 808.00
库存商品(Bioworks) WM6-40-300-003 WorkBeads 40 SEC 300ml 5,315.00
库存商品(Bioworks) WM6-40-300-010 WorkBeads 40 SEC 1L 8,734.00
库存商品(Bioworks) WM6-40-300-100 WorkBeads 40 SEC 15ml 8,397.00
库存商品(Bioworks) WM6-40-300-100S WorkBeads 40 SEC 15ML 8,152.00
库存商品(Bioworks) WM6-40-340-001S WorkBeads 40/100 SEC 25ML 920.00
库存商品(Bioworks) WM6-40-400-000 WorkBeads 40 ACT 10ml 880.00
库存商品(Bioworks) WM6-40-400-001 WorkBeads 40 ACT 50ml 3,362.00
库存商品(Bioworks) WM6-40-400-001S1 WorkBeads 40 ACT 10ml 1,520.00
库存商品(Bioworks) WM6-40-400-001S5 WorkBeads 40 ACT 5ml 760.00
库存商品(Bioworks) WM6-40-400-003 WorkBeads 40 ACT 300ml 12,772.00
库存商品(Bioworks) WM6-40-400-00X WorkBeads 40 ACT 5ml 760.00
库存商品(Bioworks) WM6-40-400-010 WorkBeads 40 ACT 1L 20,188.00
库存商品(Bioworks) WM6-40-450-001 WorksBeads 40/10 000 ACT 50ml 3,898.00
库存商品(Bioworks) WM6-40-601-000 WorkBeads 40 IDA high 10 ml 750.00
库存商品(Bioworks) WM6-40-601-001 WorkBeads 40 IDA high 25ml 1,747.00
库存商品(Bioworks) WM6-40-601-001S WorkBeads 40 IDA high 25ML 680.00
库存商品(Bioworks) WM6-40-601-003 WorkBeads 40 IDA high 150ml 6,716.00
库存商品(Bioworks) WM6-40-601-00X WorkBeads 40 IDA high 5ml 680.00
库存商品(Bioworks) WM6-40-601-010 WorkBeads 40 IDA high 1L 20,188.00
库存商品(Bioworks) WM6-40-602-001 WorkBeads 40 IDA low 25ml 1,747.00
SBO物料类别 目录号 物料描述 规格 目录价
库存商品(Bioworks) WM6-40-602-003 WorkBeads 40 IDA low 150ml 6,716.00
库存商品(Bioworks) WM6-40-602-00X WorkBeads 40 IDA low 5ml 680.00
库存商品(Bioworks) WM6-40-602-010 WorkBeads 40 IDA low 1L 20,188.00
库存商品(Bioworks) WM6-40-603-001 WorkBeads 40 TREN high 25ml 1,747.00
库存商品(Bioworks) WM6-40-603-001S5 WorkBeads 40 TREN high 5ml 680.00
库存商品(Bioworks) WM6-40-603-003 WorkBeads 40 TREN high 150ml 6,716.00
库存商品(Bioworks) WM6-40-603-00X WorkBeads 40 TREN high 5ml 680.00
库存商品(Bioworks) WM6-40-603-010 WorkBeads 40 TREN high 1L 20,188.00
库存商品(Bioworks) WM6-40-604-001 WorkBeads 40 TREN low 25ml 1,747.00
库存商品(Bioworks) WM6-40-604-003 WorkBeads 40 TREN low 150ml 6,716.00
库存商品(Bioworks) WM6-40-604-00X WorkBeads 40 TREN low 5ml 680.00
库存商品(Bioworks) WM6-40-604-010 WorkBeads 40 TREN low 1L 20,188.00
库存商品(Bioworks) WM6-40-650-000 WorkBeads™ 40 Ni Bulk Media 10 ml 880.00
库存商品(Bioworks) WM6-40-650-000-S Workbeads 40Ni Bulk Media 2 ml 150.00
库存商品(Bioworks) WM6-40-650-001 WorkBeads™ 40 Ni Bulk Media 25 ml 1,960.00
库存商品(Bioworks) WM6-40-650-003 WorkBeads™ 40 Ni Bulk Media 150 ml 10,600.00
库存商品(Bioworks) WM6-40-650-010 WorkBeads™ 40 Ni Bulk Media 1L 53,520.00
库存商品(Bioworks) WM6-50-300-000 WorkBeads 500 SEC 100ml 775.00
库存商品(CFS) WM7-EDX-PCR Premium Expression Kit, PCR 0 7,460.00
库存商品(CFS) WM7-EDX-PLUS Premium PLUS Expression Kit 0 8,600.00
库存商品(CFS) WM7-EDX-PLUS-S Premium PLUS Expression Kit 8次 8,600.00
库存商品(CFS) WM7-EDX-S Premium Expression Kit 0 6,600.00
库存商品(CFS) WM7-TRI-1240 WEPRO1240 expression kit 0 32,250.00
库存商品(CFS) WM7-TRI-1240G WEPRO1240G expression kit 0 36,650.00
库存商品(CFS) WM7-TRI-1240H WEPRO1240H expression kit 0 36,650.00
库存商品(CFS) WM7-TRI-7240 WEPRO7240 expression kit 0 42,400.00
库存商品(CFS) WM7-TRI-7240G WEPRO7240G expression kit 0 47,050.00
库存商品(CFS) WM7-TRI-7240H WEPRO7240H expression kit 0 47,050.00
库存商品(硅化) W10-0010-1 10ul Tip(硅化 不带滤芯) 1000个/包,20包/箱 80.00
库存商品(硅化) W10-0200-1 200ul Tip(硅化 不带滤芯) 1000个/包,20包/箱 80.00
库存商品(硅化) W10-1000-1 1000ul Tip(硅化 不带滤芯) 500个/包,20包/箱 62.00
库存商品(硅化) W11-0010-1 10ul 盒装Tip(硅化 不带滤芯) 96个/盒, 50盒/箱 24.00
SBO物料类别 目录号 物料描述 规格 目录价
库存商品(硅化) W11-0200-1 200ul 盒装Tip(硅化 不带滤芯) 96个/盒,50盒/箱 24.00
库存商品(硅化) W11-1000-1 1000 Tip(硅化 不带滤芯) 100个/盒, 10 盒/箱 29.00
库存商品(硅化) W12-0010-1 10ul 盒装Tip(硅化 带滤芯) 96个/盒, 50 盒/箱 58.00
库存商品(硅化) W12-0200-1 200ul 盒装Tip(硅化 带滤芯) 96个/盒,50盒/箱 58.00
库存商品(硅化) W12-1000-1 1000ul盒装 Tip(硅化 带滤芯) 100个/盒,10 盒/箱 62.00
库存商品(硅化) W20-0500-1 0.5ml硅化离心管 1000个/盒,10盒/箱 138.00
库存商品(硅化) W20-1500-1 1.5ml硅化离心管 500个/盒,10盒/箱 90.00
库存商品(硅化) W21-0200-1 0.2ml离心管(硅化) 1000个/盒,10盒/箱 196.00
库存商品(硅化) W21-0200-8RT(A) 0.2mlPCR八联管(含平盖) 125条/盒,10盒/箱 980.00
库存商品(硅化) W21-0200-8RT(B) 0.2mlPCR八联管(含凸盖) 125条/盒,10盒/箱 980.00
库存商品(硅化) W21-0500-1 0.5ml离心管(硅化) 1000个/盒,10盒/箱 180.00
库存商品(硅化) W24-1500-1 15ml离心管(硅化) 50个/包, 10包/箱 130.00
库存商品(硅化) W24-5000-1 50ml离心管(硅化) 25个/包, 20 包/箱 75.00
库存商品(硅化) WJB0101 0.2ml薄壁管(硅化) 1000个/盒,20盒/箱 70.00
库存商品(硅化) WJB0102 0.2ml PCR八联管(硅化) 1000个/盒,20盒/箱 580.00
库存商品(硅化) WJL0101 0.5ml离心管(硅化) 1000个/盒,20盒/箱 60.00
库存商品(硅化) WJL0201 1.5ml离心管(硅化) 500个/盒,20盒/箱 60.00
库存商品(硅化) WJX0101 10ul Tip(硅化 带滤芯 袋装) 1000个/包,25包/箱 350.00
库存商品(硅化) WJX0102 10ul Tip(硅化 袋装) 1000个/包,25包/箱 35.00
库存商品(硅化) WJX0201 200ul Tip(硅化 带滤芯 袋装) 1000个/包,15包/箱 350.00
库存商品(硅化) WJX0202 200ul Tip(硅化 袋装优化品) 1000个/包,15包/箱 35.00
库存商品(硅化) WJX0301 1000ul Tip(硅化 带滤芯 袋装) 500个/包,10包/箱 300.00
库存商品(硅化) WJX0302 1000ul Tip(硅化 袋装) 500个/包,10包/箱 33.00
库存商品(进口瓶子) WQ003-01-C 30ml塑料瓶(进口)(含盖) 0 5.00
库存商品(进口瓶子) WQ003-03-C 15ml塑料瓶(进口)(含盖) 0 4.00
库存商品(进口瓶子) WQ003-04-C 60ml塑料瓶(进口)(含盖) 0 6.00
库存商品(进口瓶子) WQ003-05-C 125ml塑料瓶(进口)(含盖) 0 8.50
库存商品(进口瓶子) WQ003-06-C 250ml塑料瓶(进口)(含盖) 0 12.00
库存商品(进口瓶子) WQ003-07-C 500ml塑料瓶(进口)(含盖) 0 18.50
库存商品(进口瓶子) WQ003-08-C 1000ml塑料瓶(进口)(含盖) 0 25.00
其他基因组 DP302-02 细菌基因组DNA提取试剂盒(含K)(TIANamp Bacteria DNA Kit) 50 次 420.00
其他基因组 DP302T 大量细菌基因组DNA提取试剂盒(TIANamp Maxi Bacteria DNA Kit) 10次 3,000.00
SBO物料类别 目录号 物料描述 规格 目录价
其他基因组 DP307-02 酵母基因组DNA提取试剂盒(含K)(TIANamp Yeast DNA Kit) 50 次 480.00
其他基因组 DP323-02 动物源性植物饲料DNA提取试剂盒(TIANamp Feedstuff Animal DNA Kit ) 50次 480.00
其他基因组 DP323-03 动物源性植物饲料DNA提取试剂盒(TIANamp Feedstuff Animal DNA Kit ) 200次 1,660.00
其他基因组 DP324-02 海洋动物组织基因组DNA提取试剂盒(TIANamp Marine Animals DNA Kit ) 50次 420.00
其他基因组 DP324-03 海洋动物组织基因组DNA提取试剂盒(TIANamp Marine Animals DNA Kit ) 200次 1,500.00
其他基因组 DP326 深加工食品DNA提取试剂盒(GMO Food DNA Extraction Kit ) 100次 1,200.00
其他基因组 DP328-02 粪便基因组DNA提取试剂盒(TIANamp Stool DNA Kit) 50次 980.00
其他基因组 DP336-02 土壤基因组DNA提取试剂盒 TIANamp Soil DNA Kit 50次 1,080.00
其他基因组 KG201 快速DNA提取扩增试剂盒(TIANcombi DNA Lyse&Amp PCR Kit) 试用装 60.00
其他基因组 KG201-02 快速DNA提取扩增试剂盒(TIANcombi DNA Lyse&Amp PCR Kit) 50次 600.00
其他基因组 KG201-03 快速DNA提取扩增试剂盒(TIANcombi DNA Lyse&Amp PCR Kit) 200次 1,920.00
其他基因组 MG101-01 磁珠G(MagAttract Suspension G) 1 ml 500.00
其他基因组 RK114-01 缓冲液GA(Buffer GA) 15 ml 30.00
其他基因组 RK114-02 缓冲液GA(Buffer GA) 50 ml 80.00
其他基因组 RK115-01 缓冲液GB(Buffer GB) 15 ml 30.00
其他基因组 RK115-02 缓冲液GB(Buffer GB) 50 ml 80.00
其他基因组 RK118-01 缓冲液GD(Buffer GD) 13 ml 60.00
其他基因组 RK118-02 缓冲液GD(Buffer GD) 52 ml 180.00
其他基因组 RK121-01 洗脱缓冲液TE(Buffer TE) 15 ml 25.00
其他基因组 RK121-03 洗脱缓冲液TE(Buffer TE) 60 ml 70.00
其他基因组 RK152-01 缓冲液GS(Buffer GS) 15 ml 30.00
其他基因组 RK152-02 缓冲液GS(Buffer GS) 50 ml 80.00
其他基因组 RK168 裂解液RF(Buffer RF) 12 ml 280.00
其他基因组 RT403-01 蛋白酶K(Proteinase K) 500 ul 60.00
医疗基因组 DP304-02 血液/细胞/组织基因组DNA提取试剂盒(含K)(TIANamp Genomic DNA Kit) 50 次 420.00
医疗基因组 DP304-03 血液/细胞/组织基因组DNA提取试剂盒(含K)(TIANamp Genomic DNA Kit) 200 次 1,500.00
医疗基因组 DP315 病毒基因组DNA/RNA提取试剂盒(TIANamp Virus DNA/RNA Kit) 50 次 960.00
医疗基因组 DP316 微量样品基因组DNA提取试剂盒(TIANamp Micro DNA Kit) 50 次 1,080.00
医疗基因组 DP318-02 血液基因组DNA提取试剂盒(0.1ml-1ml)(TIANamp Blood DNA Kit(0.1ml-1ml)) 50 次 420.00
医疗基因组 DP318-03 血液基因组DNA提取试剂盒(0.1ml-1ml)(TIANamp Blood DNA Kit(0.1ml-1ml)) 200 次 1,500.00
医疗基因组 DP319-01 血液基因组DNA提取系统(0.1-20ml)(RelaxGene Blood DNA System(0.1-20ml)) ml
50 380.00
医疗基因组 DP319-02 血液基因组DNA提取系统(0.1-20ml)(RelaxGene Blood DNA System(0.1-20ml)) ml
200 1,280.00
SBO物料类别 目录号 物料描述 规格 目录价
医疗基因组 DP322-02 口腔拭子基因组DNA提取试剂盒(TIANamp Swab DNA Kit) 50次 480.00
医疗基因组 DP322-03 口腔拭子基因组DNA提取试剂盒(TIANamp Swab DNA Kit) 200次 1,820.00
医疗基因组 DP329-01 磁珠法基因组DNA提取试剂盒(Magnetic Genomic DNA Kit ) 96次 1,080.00
医疗基因组 DP330-02 石蜡包埋组织DNA快速提取试剂盒(TIANquick FFPE DNA Kit) 50次 980.00
医疗基因组 DP331-02 石蜡包埋组织DNA提取试剂盒(TIANamp FFPE DNA Kit) 50次 680.00
医疗基因组 DP332-01 中量血液基因组DNA提取试剂盒(离心柱型)(TIANamp Blood DNA Midi Kit) 10次 320.00
医疗基因组 DP333-01 大量血液基因组DNA提取试剂盒(离心柱型)(TIANamp Blood DNA Maxi Kit) 10次 820.00
医疗基因组 DP334 干血斑基因组DNA提取试剂盒(TIANamp Blood Spots DNA Kit) 200次 1,820.00
医疗基因组 DP335-02 0.1-1ml血凝块基因组DNA提取试剂盒(离心柱型)(TIANamp Blood Clot DNA Kit)
50次/盒 520.00
医疗基因组 DP339 血清/血浆游离DNA提取试剂盒(离心柱型)(Serum / Plasma Circulating DNA Kit) 1,080.00
医疗基因组 DP438 磁珠法病毒DNA/RNA提取试剂盒(Magnetic Viral DNA/RNA Kit) 96次 2,980.00
医疗基因组 RK151-01 细胞裂解液CL(Buffer CL) 60 ml 50.00
医疗基因组 RK151-02 细胞裂解液CL(Buffer CL) 250 ml 120.00
医疗基因组 RK153-01 洗脱缓冲液TB(Buffer TB) 15 ml 20.00
医疗基因组 RK153-02 洗脱缓冲液TB(Buffer TB) 60 ml 50.00
医疗基因组 RK154-01 缓冲液FG(Buffer FG) 30 ml 260.00
医疗基因组 RK154-02 缓冲液FG(Buffer FG) 120 ml 880.00
仪器(TGem) OSE-260 分光光度计 0 58,000.00
仪器(小仪器) OSE-100C 升降温型金属浴(0-100℃) 0 7,180.00
仪器(小仪器) OSE-470 切胶仪 0 5,800.00
仪器(小仪器) OSE-96 金属冰浴 0 4,180.00
仪器(小仪器) OSE-B1 研磨器电池 0 110.00
仪器(小仪器) OSE-B2 研磨器充电器 0 60.00
仪器(小仪器) OSE-B2 研磨器充电器 个 60.00
仪器(小仪器) OSE-H 加热型金属浴(室温2-100℃) 0 3,480.00
仪器(小仪器) OSE-H15 15mL 铝块 FBLOCK-15 0 620.00
仪器(小仪器) OSE-H50 50mL 铝块 FBLOCK-50 0 620.00
仪器(小仪器) OSE-JSC 金属研磨杵 0 58.00
仪器(小仪器) OSE-MC8 TGear微型离心机 0 1,680.00
仪器(小仪器) OSE-MP25PS TGear Plate微孔板离心机套装 0 4,880.00
仪器(小仪器) OSE-P96 96孔平板铝块 FBLOCK-96 0 930.00
仪器(小仪器) OSE-PRO 程控金属浴(时间控制,0-100℃) 0 7,980.00
SBO物料类别 目录号 物料描述 规格 目录价
仪器(小仪器) OSE-PROIII 三段程控金属浴(时间控制,0-100度)H2O3-PRO III 0 9,580.00
仪器(小仪器) OSE-Q0.2 0.2mL 铝块 QBLOCK-0.2 0 260.00
仪器(小仪器) OSE-Q0.5 0.5mL 铝块 QBLOCK-0.5 0 260.00
仪器(小仪器) OSE-Q1.5 1.5mL 铝块 QBLOCK-1.5 0 260.00
仪器(小仪器) OSE-Q2.0 2.0mL 铝块 QBLOCK-2.0 0 260.00
仪器(小仪器) OSE-Y10 电动组织研磨器(高转速) 套 1,790.00
仪器(小仪器) OSE-Y20 电动组织研磨器(低转速) 套 1,790.00
仪器(小仪器ESE) OSE-BR ESEQuant Barcode 2D 0 3,800.00
仪器(小仪器ESE) OSE-DK-FL ESEQuant Development Kit FL 0 68,000.00
仪器(小仪器ESE) OSE-DK-GD ESEQuant Development Kit Gold 0 63,000.00
仪器(小仪器ESE) OSE-EPJ ESE配件 0 10,000.00
仪器(小仪器ESE) OSE-FL ESEQuant LFR FL 0 25,000.00
仪器(小仪器ESE) OSE-GD ESEQuant LFR Gold 0 20,000.00
仪器(小仪器ESE) OSE-ID FluoSens Integrated Detector 0 18,532.80
仪器(小仪器ESE) OSE-TS TubeScanner 0 58,000.00
植物基因组 DP305-02 植物基因组DNA提取试剂盒(Plant Genomic DNA Kit) 50 次 420.00
植物基因组 DP305-03 植物基因组DNA提取试剂盒(Plant Genomic DNA Kit) 200 次 1,500.00
植物基因组 DP320-02 DNAsecure新型植物基因组DNA提取试剂盒(DNAsecure Plant Kit) 50 次 520.00
植物基因组 DP320-03 DNAsecure新型植物基因组DNA提取试剂盒(DNAsecure Plant Kit) 200 次 1,680.00
植物基因组 DP321-02 快捷型植物基因组DNA提取系统(DNAquick Plant System) 50 次 280.00
植物基因组 DP321-03 快捷型植物基因组DNA提取系统(DNAquick Plant System) 200 次 880.00
植物基因组 kg202 GMO作物提取检测试剂盒 200次 1,280.00
植物基因组 KG202-A GMO作物提取检测试剂盒(A部分)(GMO Crop Extraction & Amplification Kit(Part A))
200次 580.00
植物基因组 KG202-B GMO作物提取检测试剂盒(B部分)(GMO Crop Extraction & Amplification Kit(Part B))
400U(2.5U/ul) 980.00
植物基因组 RK116-01 缓冲液GP1(Buffer GP1) 40 ml 40.00
植物基因组 RK116-02 缓冲液GP1(Buffer GP1) 160 ml 120.00
植物基因组 RK117-01 缓冲液GP2(Buffer GP2) 40 ml 40.00
植物基因组 RK117-02 缓冲液GP2(Buffer GP2) 160 ml 120.00
植物基因组 RK155-01 缓冲液LP1/FP1 (Buffer LP1/FP1) 25 ml 60.00
植物基因组 RK155-02 缓冲液LP1/FP1 (Buffer LP1/FP1) 100 ml 200.00
植物基因组 RK156-01 缓冲液LP2/FP2 (Buffer LP2/FP2) 10 ml 30.00
植物基因组 RK156-02 缓冲液LP2/FP2(Buffer LP2/FP2) 40 ml 100.00
SBO物料类别 目录号 物料描述 规格 目录价
植物基因组 RK157-01 缓冲液LP3(Buffer LP3) 21 ml 60.00
植物基因组 RK157-02 缓冲液LP3(Buffer LP3) 84 ml 200.00
植物基因组 RK160 缓冲液GMO1(Buffer GMO1) 50 ml 700.00
植物基因组 RK161 缓冲液GMO2(Buffer GMO2) 20 ml 300.00
质粒大提 DP116 高纯度质粒大提试剂盒(非离心柱型)(HighPure Maxi Plasmid Kit) 10次 680.00
质粒大提 DP117 无内毒素质粒大提试剂盒(离心柱型)(EndoFree Maxi Plasmid Kit) 10 次 980.00
质粒大提 RK109-03 溶液P4(Buffer P4) 100 ml 480.00
质粒大提 RK147 TIANRed 500 ul 30.00
质粒小提 DP103-02 离心柱型 质粒小提试剂盒(TIANprep Mini Plasmid Kit) 50次 190.00
质粒小提 DP103-03 离心柱型 质粒小提试剂盒(TIANprep Mini Plasmid Kit) 200 次 680.00
质粒小提 DP104-02 离心柱型 高纯度质粒小提试剂盒(TIANpure Mini Plasmid Kit) 50 次 300.00
质粒小提 DP105-02 快速质粒小提试剂盒(离心柱型)(TIANprep Rapid Mini Plasmid Kit) 50次 190.00
质粒小提 DP105-03 快速质粒小提试剂盒(离心柱型)(TIANprep Rapid Mini Plasmid Kit) 200次 680.00
质粒小提 DP106-02 离心柱型 质粒小提中量试剂盒(TIANprep Midi Plasmid Kit) 50 次 300.00
质粒小提 DP107-02 离心柱型 高纯度质粒小提中量试剂盒(TIANpure Midi Plasmid Kit) 50 次 360.00
质粒小提 DP112-02 离心柱型 酵母质粒提取试剂盒(TIANprep Yeast Plasmid DNA Kit) 50 次 300.00
质粒小提 DP118-02 无内毒素质粒小提中量试剂盒(离心柱型)(EndoFree Midi Plasmid Kit) 50次 580.00
质粒小提 RK101-01 溶液P1(Buffer P1) 15 ml 20.00
质粒小提 RK101-02 溶液P1(Buffer P1) 60 ml 60.00
质粒小提 RK101-03 溶液P1(Buffer P1) 100 ml 100.00
质粒小提 RK101-04 溶液P1(Buffer P1) 400 ml 360.00
质粒小提 RK102-01 溶液P2(Buffer P2) 15 ml 20.00
质粒小提 RK102-02 溶液P2(Buffer P2) 60 ml 60.00
质粒小提 RK102-03 溶液P2(Buffer P2) 100 ml 100.00
质粒小提 RK102-04 溶液P2(Buffer P2) 400 ml 360.00
质粒小提 RK103-01 溶液P3(Buffer P3) 20 ml 40.00
质粒小提 RK103-02 溶液P3(Buffer P3) 80 ml 120.00
质粒小提 RK104-02 溶液III(Buffer III) 60 ml 60.00
质粒小提 RK104-03 溶液III(Buffer III) 400 ml 360.00
质粒小提 RK105-01 去蛋白液PD(Buffer PD) 30 ml 70.00
质粒小提 RK105-02 去蛋白液PD(Buffer PD) 120 ml 250.00
质粒小提 RK113-01 漂洗液PW(Buffer PW) 15 ml 20.00
SBO物料类别 目录号 物料描述 规格 目录价
质粒小提 RK113-02 漂洗液PW(Buffer PW) 50 ml 48.00
质粒小提 RK120-01 洗脱缓冲液EB(Buffer EB) 15 ml 25.00
质粒小提 RK120-02 洗脱缓冲液EB(Buffer EB) 30 ml 30.00
质粒小提 RK140 溶液YP1(Buffer YP1) 15 ml 30.00
质粒小提 RK141 溶液YP2(Buffer YP2) 15 ml 30.00
质粒小提 RK142 溶液YP3(Buffer YP3) 20 ml 40.00
质粒小提 RK150 平衡液BL(Buffer BL) 15ml 20.00
质粒小提 RK150-01 平衡液BL(Buffer BL) 30 ml 30.00
质粒小提 RK150-03 平衡液BL(Buffer BL) 120 ml 110.00
质粒小提 RT405-01 RNaseA(10mg/ml) 200 ul 30.00
质粒小提 RT405-02 RNaseA(10mg/ml) 1000 ul 80.00
质粒小提 RT405-04 RNaseA 150 ul 30.00
质粒小提 RT405-06 RNaseA(10mg/ml) 600 ul 80.00
转染试剂 RM201-01 Lipofect Transfection Reagent 0.5 ml 800.00
转染试剂 RM201-02 Lipofect Transfection Reagent 5ml 7,000.00
转染试剂 RM202-01 DNAfectin Transfection Reagent 0.75ml 1,700.00
转染试剂 RM202-02 DNAfectin Transfection Reagent 4×0.75ml 6,000.00
转染试剂 RM203-01 RNAfectin Transfection Reagent 0.75ml 1,700.00
转染试剂 RM203-02 RNAfectin Transfection Reagent 4×0.75ml 6,000.00
转染试剂 RM204-01 TianFect Transfection Reagent/TianFect转染试剂 750µl 3,050.00
转染试剂 RM204-02 TianFect Transfection Reagent/TianFect转染试剂 2×750µl 5,000.00更多产品,更多优惠,请联系我们!
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Email:sales@jinpanbio.comharlan teklad官网 TD.88051和TD.90221 TD.94059 胆固醇和胆酸盐的混合高脂饮食
harlan teklad官网 TD.88051和TD.90221 TD.94059 胆固醇和胆酸盐的混合高脂饮食
贝弗利·派根和他的同事首先通过喂养一种混合性动脉粥样硬化饮食来描述C57BL/6小鼠动脉粥样硬化的发展。这种混合饲料是通过将一种天然成分的老鼠饲料按3:1的比例与浓缩的纯化饲料(含5%胆固醇和2%胆酸钠;称为Thoms-Hartroft饮食)混合而成的。产生的混合物在TD.88051/TD.90221(同配方)含有~15.8%脂肪、1.25%胆固醇和0.5%胆酸钠。这组人后来将混合动脉粥样硬化饮食方法与更现代的“西方”纯化动脉粥样硬化饮食方法进行了比较,并添加了胆固醇和胆酸盐,发现混合动脉粥样硬化饮食会导致更多的胆结石和肝脏损伤。混合饮食含有多种未精制的成分,这些成分可能会改变血脂代谢和动脉粥样硬化的发生,并且不允许对成分和营养物进行精确控制,以用于慢性病的研究。虽然已经开发出了更精细的饮食,但混合动脉粥样硬化饮食仍然是野生型小鼠和大鼠诱发轻度动脉粥样硬化和胆结石的流行食品。联系我们获得更多的信息,修改,或可能的控制饮食。
添加胆固醇和胆酸盐的混合高脂饮食实例*:
添加胆固醇的混合高脂饮食的例子(没有胆酸来源):
研究用途:
主要在野生型小鼠和大鼠诱发高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。
不会助长肥胖。
也用于造石(胆石)啮齿动物的研究。
主要饮食特征:
- 75%的啮齿动物饲养员;25%的纯化成分
- 高脂肪(体重约15%;脂肪37%千卡)
- 饱和脂肪酸(SFA>总脂肪酸的45%)
- 胆固醇(1.25%)
- 巧克力源(0.5%)*
参考资料:
- Nishina,下午,J.Verstuyft,B.Paigen,合成低脂和高脂肪饮食,用于研究小鼠动脉粥样硬化。j Lipid RES,1990年。31(5):P。859-69
- Clee,S.M.,等,血浆和血管壁脂蛋白脂肪酶在动脉粥样硬化中有不同的作用。j Lipid RES,2000年。41(4):P。521-31
- George,J.等人,用热休克蛋白-65免疫C57BL/6J小鼠,促进了脂肪条纹的形成。Arteroscler Thromb VASc Biol,1999年。19(3):P。505-10
- Miyake,J.H.,等,胆固醇-7-α-羟化酶基因表达对C57BL/6J小鼠动脉粥样硬化的预防作用。Arteroscler Thromb VASc Biol,2002年。22(1):P。121-6
- Paigen,B,等,小鼠动脉粥样硬化病变的定量评估。动脉粥样硬化,1987年。68(3):P。231-40。
- Schreyer,S.A.,D.L.Wilson和R.C.LeBoeuf,C57BL/6小鼠以高脂饮食作为糖尿病加速动脉粥样硬化的模型。动脉粥样硬化,1998年。136(1):P。17-24。
- Vergnes,L,等,胆固醇和胆酸组成的动脉粥样硬化饮食诱导不同阶段的肝脏炎症基因的表达。j Biol Chem,2003年。278(44):P。42774-84。
*胆酸钠或胆酸有助胆固醇和脂肪的吸收,并可透过胆汁酸合成减少胆固醇的排出。然而,如果你的研究不想包括胆酸盐的来源,没有胆酸盐的饮食是可用的。看见TD.96121为了纯正的饮食TD.94059混合饮食。请与我们联系,了解其他选项。
Hybrid high fat diets with added cholesterol and cholate source*
Beverly Paigen and colleagues first characterized atherosclerosis development in C57BL/6 mice by feeding a hybrid atherogenic diet. The hybrid diet was created by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate; referred to as Thomas-Hartroft diet). The resulting mixture recreated in TD.88051/TD.90221 (same formula) contains ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. This group later compared the hybrid atherogenic diet approach to the more modern “western” purified atherogenic diet with added cholesterol and cholate and found that the hybrid atherogenic diet induced more gallstones and liver damage. Hybrid diets contain a variety of unrefined ingredients that may modify lipid metabolism and atherogenesis and do not allow for precise control of ingredients and nutrients for the study of chronic diseases. Although more refined diets have been developed, hybrid atherogenic diets are still popular for inducing mild atherosclerosis and gallstones in wild type mice and rats. Contact us for more information, modifications, or possible control diets.
Examples of hybrid high-fat diets with added cholesterol and cholate source*:
Example of hybrid high-fat diet with added cholesterol (without cholate source):
Research Use:
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.
Will not promote obesity.
Also used for lithogenic (gallstone) rodent studies.
Key dietary features:
- 75% rodent breeder diet; 25% purified ingredients
- High fat (~15% by weight; 37% kcal from fat)
- Saturated fatty acids (SFA >45% of total fatty acids)
- Cholesterol (1.25%)
- Cholate source (0.5%)*
References:
- Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69.
- Clee, S.M., et al., Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis. J Lipid Res, 2000. 41(4): p. 521-31.
- George, J., et al., Enhanced fatty streak formation in C57BL/6J mice by immunization with heat shock protein-65. Arterioscler Thromb Vasc Biol, 1999. 19(3): p. 505-10.
- Miyake, J.H., et al., Transgenic expression of cholesterol-7-alpha-hydroxylase prevents atherosclerosis in C57BL/6J mice. Arterioscler Thromb Vasc Biol, 2002. 22(1): p. 121-6.
- Paigen, B., et al., Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis, 1987. 68(3): p. 231-40.
- Schreyer, S.A., D.L. Wilson, and R.C. LeBoeuf, C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis. Atherosclerosis, 1998. 136(1): p. 17-24.
- Vergnes, L., et al., Cholesterol and cholate components of an atherogenic diet induce distinct stages of hepatic inflammatory gene expression. J Biol Chem, 2003. 278(44): p. 42774-84.
*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available. See TD.96121 for a purified diet and TD.94059 for a hybrid diet. Contact us for additional options.
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