Yes.  If the assay format is not available for your auto-analyser you can supply the following parameters of your relevant auto-analyser to Megazyme and an appropriate assay format may be available:
Parameters:
Instrument make and model:
Light-path length:
Reagent addition volumes (e.g. R1 volume, R2 volume, R3 if applicable):
Suggested auto-analyser format for K-ASCO:
Prepare the reagents as described in the K-ASCO data booklet then prepare R1 and R2 as follows:
Preparation of R1: (90 assays)

Preparation of R2: (90 assays)

EXAMPLE METHOD:
R1: 0.260 mL
Sample: ~ 0.005 mL
R2: 0.05 mL
Note: For accurate measurement of ascorbic acid each sample requires two measurements, one with AAO (bottle 4) present and one with AAO (bottle 4) absent.
In the auto-analyser format described above there is no option to read A1 as in the cuvette assay.  In this instance use an ascorbic acid calibration curve to calculate results.  Alternatively add 0.025 mL of bottle 2 and 3 separately to mimic the cuvette assay and allow the reading  A1 (this will require 3 reagent additions) OR it may be possible to add the MTT (bottle 2) with R1 as follows:
Preparation of R1: (90 assays) 

Preparation of R2: (90 assays)

EXAMPLE METHOD:
R1: 0.285 mL
Sample: ~ 0.005 mL
R2: 0.025 mL 

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
3. State the kit lot number being used (this is found on the outside of the kit box).
4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
6. State the sample type and describe the sample preparation steps if applicable.

Yes.  K-XYLOSE can be used to measure D-xylose in urine samples assuming that the D-xylose concentration is above the limit of detection for the kit assay after sample preparation.  An example sample preparation method for biological samples is provided in the K-XYLOSE data booklet. 

K-XYLOSE is able to specifically measure xylose in samples that contain up to 5 mg of glucose in the final assay reaction without any interference by the glucose.

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.