–SulfoBiotics– Biotin-HPDP(WS) solution

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1) S. R. Jaffrey and Solomon H. Snyder, "The biotin switch method for the detection of S-nitrosylated proteins ", Sci. STKE, 2001, 86, pl1.
2) X. Wang, N. Kettenhofen, S. Shiva, N. Hogg, and M. Gladwin, "Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins", Free Radic. Biol. Med., 2008, 44, 1362.
3) M. T. Forrester, M. W. Foster, M. Benhar, and J. S. Stamler, "Detection of protein S-nitrosylation with the biotin switch technique", Free Radic. Biol. Med., 2009, 46(2), 119.
4) M. D. Kornberg, N. Sen, M. R. Hara, K. R. Juluri, J. V. K. Nguyen, A. M. Snowman, L. Law, L. D. Hester, and S. H. Snyder, "GAPDH mediates nitrosylation of nuclear proteins", Nat. Cell Biol., 2010, 12(11), 1094.
5) J. Wan, A. F. Roth, A. O. Bailey, and N. G. Davis, "Palmitoylated proteins: purification and identification", Nat. Protoc., 2007, 1573.
6) A. K. Mustafa, M. M. Gadalla, N. Sen, S. Kim, W. Mu, S. K. Gazi, R. K. Barrow, G. Yang, R. Wang, and S. H. Snyder, "H2S signals through protein S-sulfhydration", Sci. Signal., 2009, 2(96), ra72.

MQAE

• プロトコル 細胞内塩化物イオンを測定したい
  

1) A. S. Verkman, M. C. Sellers, A. C. Chao, T. Leung and R. Ketcham, "Synthesis and Characterization of Improved Chloride-sensitive Fluorescent Indicators for Biological Applications", Anal. Biochem., 1989, 178, 355. 
2) M. Inoue, M. Hara, X.-T. Zeng, T. Hirose, S. Ohnishi, T. Yasukura, T. Uriu, K. Omori, A. Minato and C. Inagaki, "An ATP-driven Cl^– Pump Regulates Cl^– Concentrations in Rathippocampal Neurons", Neurosci. Lett., 1991, 134, 75. 
3) T. Nakamura, H. Kaneko and N. Nishida, "Direct Measurement of Chloride Concentration in Newt Olfactory Receptors with the Fluorescent Probe", Neurosci. Lett., 1997, 237, 5.

4H(EDTA・free acid)

–SulfoBiotics– PEG-PCMal

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• プロトコル
  

種々の試料中に含まれるタンパク質のチオール解析に適用される。汎用されているPEG-Maleimideを用いた参考文献を各試料ごとに示した。
　参考文献 1): 精製タンパク質
　参考文献 2): 細胞
　参考文献 3): 組織
　参考文献 4): 菌

1) L. Makmura, M. Hamann, A. Areopagita, S. Furuta, A. Munoz and J. Momand. , "Development of a sensitive assay to detect reversibly oxidized protein cysteine sulfhydryl groups", Antioxid Redox Signal., 2001, 3, (6), 1105. (精製タンパク質)
2) HH Wu, J.A. Thomas and J. Momand, "p53 protein oxidation in cultured cells in response to pyrrolidine dithiocarbamate: a novel method for relating the amount of p53 oxidation in vivo to the regulation of p53-responsive genes", Biochem J., 2000, 351, 87. (細胞)
3) JR. Burgoyne, O. Oviosu and P. Eaton., "The PEG-switch assay: A fast semi-quantitative method to determine protein reversible cysteine oxidation", J Pharmacol Toxicol Methods., 2013, 68, (3), 297. (組織)
4) L. JTetsch, C. Koller, A. Donhofer and K. Jung, "Detection and function of an intramolecular disulfide bond in the pH-responsive CadC of Escherichia coli", BMC Microbiol., 2011, doi: 10.1186/1471-2180-11-74. (菌)
5) Kazuyoshi Yamauchi, "Redox status of serum apolipoprotein E and its impact on HDL cholesterol levels", Clinical Biochemistry., 2017.

–SulfoBiotics– PEG-PCMal

• 取扱説明書 日本語
  
• 取扱説明書 English
  
• プロトコル
  

種々の試料中に含まれるタンパク質のチオール解析に適用される。汎用されているPEG-Maleimideを用いた参考文献を各試料ごとに示した。
　参考文献 1): 精製タンパク質
　参考文献 2): 細胞
　参考文献 3): 組織
　参考文献 4): 菌

1) L. Makmura, M. Hamann, A. Areopagita, S. Furuta, A. Munoz and J. Momand. , "Development of a sensitive assay to detect reversibly oxidized protein cysteine sulfhydryl groups", Antioxid Redox Signal., 2001, 3, (6), 1105. (精製タンパク質)
2) HH Wu, J.A. Thomas and J. Momand, "p53 protein oxidation in cultured cells in response to pyrrolidine dithiocarbamate: a novel method for relating the amount of p53 oxidation in vivo to the regulation of p53-responsive genes", Biochem J., 2000, 351, 87. (細胞)
3) JR. Burgoyne, O. Oviosu and P. Eaton., "The PEG-switch assay: A fast semi-quantitative method to determine protein reversible cysteine oxidation", J Pharmacol Toxicol Methods., 2013, 68, (3), 297. (組織)
4) L. JTetsch, C. Koller, A. Donhofer and K. Jung, "Detection and function of an intramolecular disulfide bond in the pH-responsive CadC of Escherichia coli", BMC Microbiol., 2011, doi: 10.1186/1471-2180-11-74. (菌)
5) Kazuyoshi Yamauchi, "Redox status of serum apolipoprotein E and its impact on HDL cholesterol levels", Clinical Biochemistry., 2017.

Mito-FerroGreen

• 取扱説明書 日本語
  
• 取扱説明書 English
  
• プロトコル ミトコンドリアの鉄イオンを検出したい
  

参考文献

1) T. Hirayama,  S. Kadota, M. Niwa  and  H. Nagasawa, "A mitochondria-targeted fluorescent probe for selective detection of mitochondrial labile Fe(II)", Metallomics., 2018, DOI: 10.1039/C8MT00049B, ※論文中では「Mito-FerroGreen」を「Ac-MtFluNox」で表記。.
2) T. Issitt, E. Bosseboeuf, N. Winter, N. Dufton, G. Gestri, V. Senatore, A. Chikh, A. Randi, C. Raimondi, "Neuropilin-1 controls endothelial homeostasis by regulating mitochondrial function and iron-dependent oxidative stress via ABCB8", iScience., 2018,DOI: 10.1016/j.isci.2018.12.005 .
3) E. E. Mon, F. Y. Wei, R. N. R. Ahmad, T. Yamamoto, T. Moroishi and K. Tomizawa, "Regulation of mitochondrial iron homeostasis by siderofexin 2 ", J Physiol Sci., 2018,doi:10.1007/s12576-018-0652-2.
4) M. Fujimaki, N. Furuya, S. Saiki, T. Amo, Y. Imamichi and N. Hattori, "Iron supply via NCOA4-mediated ferritin degradation maintains mitochondrial functions", Mol. Cell. Biol.., 2019,doi: 10.1128/MCB.00010-19.
5) K. Tomita, M. Fukumoto, K. Itoh, Y. Kuwahara, K. Igarashi, T. Nagasawa, M. Suzuki, A. Kurimasa and T. Sato, "MiR-7-5p is a key factor that controls radioresistance via intracellular Fe2+ content in clinically relevant radioresistant cells.", Biochem Biophys Res Commun.., 2019,doi: 10.1016/j.bbrc.2019.08.117.
6) Y. Wang and M. Tang, "PM2.5 induces ferroptosis in human endothelial cells through iron overload and redox imbalance", Environ. Pollut., 2019, 264, doi: 10.1016/j.envpol.2019.07.105.
7) KF. Yambire, C. Rostosky, T. Watanabe, D. Pacheu-Grau, S. Torres-Odio,A. Sanchez-Guerrero,O. Senderovich, EG. Meyron-Holtz,I.Milosevic, J. Frahm, AP. West and N. Raimundo, "Impaired lysosomal acidification triggers iron deficiency and inflammation in vivo.", Elife, 2019, 3, (8), doi:10.7554/eLife.51031. 
8) H. Nishizawa, M. Matsumoto, T. Shindo, D. Saigusa, H. Kato, K. Suzuki, M. Sato, Y. Ishii, H. Shimokawa and K. Igarashi, "Ferroptosis is controlled by the coordinated transcriptional regulation of glutathione and labile iron metabolism by the transcription factor BACH1",  J. Biol. Chem.,  2019,doi: 10.1074/jbc.RA119.009548.
9)Y. akashima, A. Hayano and B. Yamanaka, Metabolome analysis reveals excessive glycolysis via PI3K/AKT/mTOR and RAS/MAPK signaling in methotrexate-resistant primary CNS lymphoma-derived cells.", Clin. Cancer Res., 2020, DOI:10.1158/1078-0432.

2K(EDTA・2K)

–SulfoBiotics– HSip-1

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1) K.Sasakura, K.Hanaoka, N.Shibuya, Y.Mikami, Y. Kimura, T.Komatsu, T.Ueno, T.Terai, H.Kimura, and Tetsuo Nagano, "Development of a Highly Selective Fluorescence Probe for Hydrogen Sulfide", J. Am. Chem. Soc., 2011, 133, (45), 18003–18005.
2) H.Kimura, N.Shibuya, and Y.Kimura, "Hydrogen Sulfide Is a Signaling Molecule and a Cytoprotectant", Antioxid Redox Signal., 2012, 17, (1), 45-47.
3) H.Jurkowska, Heather B. Roman, Lawrence L. Hirschberger, K.Sasakura, T.Nagano, K.Hanaoka, J.Krijt, and Martha H. Stipanuk, "Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate", Amino Acids., 2014, 48, (5), 1353-1365.
4) E.Marutani, M. Sakaguchi, W.Chen, K.Sasakura,c J. Liu, M.Xian, K. Hanaoka, T.Nagano, and F.Ichinose, "Cytoprotective effects of hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonists are mediated by intracellular sulfane sulfur", Medchemcomm., 2014, 5, (10), 1577-1583.
5) N.Fukushima, N.Ieda, M.Kawaguchi, K. Sasakura, T.Nagano, K.Hanaoka, N.Miyata, H.Nakagawa, "Development of photo-controllable hydrogen sulfide donor applicable in live cells", Bioorganic & Medicinal Chemistry Letters., 2015, 25, (2), 175-178.
6) E.Marutani, M.Yamada, T.Ida, K.Tokuda, K.Ikeda, S.Kai, K.Shirozu, K.Hayashida, S.Kosugi, K.Hanaoka, M.Kaneki, T.Akaike, and F.Ichinose, "Thiosulfate Mediates Cytoprotective Effects of Hydrogen Sulfide Against Neuronal Ischemia", J Am Heart Assoc., 2015, 4, (11), e002125.
7) K.Hanaoka, K.Sasakura, Y.Suwanai, S.Toma-Fukai, K.Shimamoto, Y..akano, N.Shibuya, T.Terai, T.Komatsu, T. Ueno, Y.Ogasawara, Y.Tsuchiya Y.Watanabe, H.Kimura, C.Wang, M.Uchiyama, H.Kojima, T.Okabe, Y.Urano, T.Shimizu & Tetsuo Nagano, "Discovery and Mechanistic Characterization of Selective Inhibitors of H2S-producing Enzyme: 3-Mercaptopyruvate Sulfurtransferase (3MST) Targeting Active-site Cysteine Persulfide", Scientific Reports 7., 2017, 40227, (10), 1038.
8)Y.Kawahara, Y.Hirashita, C.Tamura, Y.Kudo, K.Sakai, K.Togo, K.Fukuda, O.Matsunari, K.Okamoto, R.Ogawa, K.Mizukami, T.Okimoto, M.Kodama, K.Murakami, "Helicobacter pylori infection modulates endogenous hydrogen sulfide production in gastric cancer AGS cells", Helicobacter, 2020,  https://doi.org/10.1111/hel.12732.

–SulfoBiotics– HSip-1

• 取扱説明書 日本語
  
• 取扱説明書 English
  

1) K.Sasakura, K.Hanaoka, N.Shibuya, Y.Mikami, Y. Kimura, T.Komatsu, T.Ueno, T.Terai, H.Kimura, and Tetsuo Nagano, "Development of a Highly Selective Fluorescence Probe for Hydrogen Sulfide", J. Am. Chem. Soc., 2011, 133, (45), 18003–18005.
2) H.Kimura, N.Shibuya, and Y.Kimura, "Hydrogen Sulfide Is a Signaling Molecule and a Cytoprotectant", Antioxid Redox Signal., 2012, 17, (1), 45-47.
3) H.Jurkowska, Heather B. Roman, Lawrence L. Hirschberger, K.Sasakura, T.Nagano, K.Hanaoka, J.Krijt, and Martha H. Stipanuk, "Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate", Amino Acids., 2014, 48, (5), 1353-1365.
4) E.Marutani, M. Sakaguchi, W.Chen, K.Sasakura,c J. Liu, M.Xian, K. Hanaoka, T.Nagano, and F.Ichinose, "Cytoprotective effects of hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonists are mediated by intracellular sulfane sulfur", Medchemcomm., 2014, 5, (10), 1577-1583.
5) N.Fukushima, N.Ieda, M.Kawaguchi, K. Sasakura, T.Nagano, K.Hanaoka, N.Miyata, H.Nakagawa, "Development of photo-controllable hydrogen sulfide donor applicable in live cells", Bioorganic & Medicinal Chemistry Letters., 2015, 25, (2), 175-178.
6) E.Marutani, M.Yamada, T.Ida, K.Tokuda, K.Ikeda, S.Kai, K.Shirozu, K.Hayashida, S.Kosugi, K.Hanaoka, M.Kaneki, T.Akaike, and F.Ichinose, "Thiosulfate Mediates Cytoprotective Effects of Hydrogen Sulfide Against Neuronal Ischemia", J Am Heart Assoc., 2015, 4, (11), e002125.
7) K.Hanaoka, K.Sasakura, Y.Suwanai, S.Toma-Fukai, K.Shimamoto, Y..akano, N.Shibuya, T.Terai, T.Komatsu, T. Ueno, Y.Ogasawara, Y.Tsuchiya Y.Watanabe, H.Kimura, C.Wang, M.Uchiyama, H.Kojima, T.Okabe, Y.Urano, T.Shimizu & Tetsuo Nagano, "Discovery and Mechanistic Characterization of Selective Inhibitors of H2S-producing Enzyme: 3-Mercaptopyruvate Sulfurtransferase (3MST) Targeting Active-site Cysteine Persulfide", Scientific Reports 7., 2017, 40227, (10), 1038.
8)Y.Kawahara, Y.Hirashita, C.Tamura, Y.Kudo, K.Sakai, K.Togo, K.Fukuda, O.Matsunari, K.Okamoto, R.Ogawa, K.Mizukami, T.Okimoto, M.Kodama, K.Murakami, "Helicobacter pylori infection modulates endogenous hydrogen sulfide production in gastric cancer AGS cells", Helicobacter, 2020,  https://doi.org/10.1111/hel.12732.

BAPTA

• プロトコル 蛍光で細胞内 Ca を測定したい
  

–SulfoBiotics– HSip-1 DA

• 取扱説明書 日本語
  
• 取扱説明書 English
  

1) K.Sasakura, K.Hanaoka, N.Shibuya, Y.Mikami, Y. Kimura, T.Komatsu, T.Ueno, T.Terai, H.Kimura, and Tetsuo Nagano, "Development of a Highly Selective Fluorescence Probe for Hydrogen Sulfide", J. Am. Chem. Soc., 2011, 133, (45), 18003–18005.
2) H.Kimura, N.Shibuya, and Y.Kimura, "Hydrogen Sulfide Is a Signaling Molecule and a Cytoprotectant", Antioxid Redox Signal., 2012, 17, (1), 45-47.
3) E.Marutani, M. Sakaguchi, W.Chen, K.Sasakura,c J. Liu, M.Xian, K. Hanaoka, T.Nagano, and F.Ichinose, "Cytoprotective effects of hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonists are mediated by intracellular sulfane sulfur", Medchemcomm., 2014, 5, (10), 1577-1583.
4) N.Fukushima, N.Ieda, M.Kawaguchi, K. Sasakura, T.Nagano, K.Hanaoka, N.Miyata, H.Nakagawa, "Development of photo-controllable hydrogen sulfide donor applicable in live cells", Bioorganic & Medicinal Chemistry Letters., 2015, 25, (2), 175-178.
5) E.Marutani, M.Yamada, T.Ida, K.Tokuda, K.Ikeda, S.Kai, K.Shirozu, K.Hayashida, S.Kosugi, K.Hanaoka, M.Kaneki, T.Akaike, and F.Ichinose, "Thiosulfate Mediates Cytoprotective Effects of Hydrogen Sulfide Against Neuronal Ischemia", J Am Heart Assoc., 2015, 4, (11), e002125.

3K(EDTA・3K)

2NA(EDTA・2Na)

DPPH Antioxidant Assay Kit

• 取扱説明書 日本語
  
• 取扱説明書 English
  

1) Enjuro Harunari, Chiaki Imada, Yasuhiro Igarashi, Konamycins A and B and Rubromycins CA1 and CA2, Aromatic Polyketides from the Tunicate-Derived Streptomyces hyaluromycini MB-PO13^T", J. Nat. Prod.., 2019, 82, (6), 1609-1615.

BAPTA-AM solution

1) R. Y. Tsien, "New Calcium Indicators and Buffers with High Selectivity against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures", Biochemistry, 1980, 19 (11), 2396.
2) R. Y. Tsien, "A Non-disruptive Technique for Loading Calcium Buffers and Indicators into Cells", Nature, 1981, 290, 527.
3) J. I. Korenbrot, D. L. Ochs, J. A. Williams, D. L. Miller and J. E. Brown, "The Use of Tetracarboxylate Fluorescent Indicators in the Measurement and Control of Intracellular Free Calcium Ions", Soc. Gen. Physiol. Ser., 1986, 40, 347.
4) S. M. Harrison and D. M. Bers, "The Effect of Temperature and Ionic Strength on the Apparent Ca-affinity of EGTA and the Analogous Ca-chelators BAPTA and Dibromo-BAPTA", Biochim. Biophys. Acta, 1987, 925, 133.
5) E. W. Gelfand and R. K. Cheung, "Dissociation of Unidirectional Influx of External Ca²⁺ and Release from Internal Stores in Activated Human T Lymphocytes", Eur. J. Immunol., 1990, 20, 1237.
6) J. P. Kao, J. M. Alderton, R. Y. Tsien and R. A. Steinhardt, "Active Involvement of Ca²⁺ in Mitotic Progression of Swiss 3T3 Fibroblasts", J. Cell Biol., 1990, 111, 183.
7) M. L. Schubert and J. Hightower, "Functionally Distinct Muscarinic Receptors on Gastric Somatostatin Cells", Am. J. Physiol., 1990, 258, G982.
8) Y. Tojyo and Y. Matsumoto, "Inhibitory Effects of Loading with the Calcium-chelator 1, 2-Bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) on Amylase Release and Cellular ATP Level in Rat Parotid Cells", Biochem. Pharmacol., 1990, 39(11), 1775.

3NA(EDTA・3Na)

Caged ATP

• プロトコル 細胞内目的部位にATP を局部添加したい
  

1) J. H. Kaplan, B. F. Iii and J. F. Hoffman, "Rapid Photolytic Release of Adenosine 5'-triphosphate from a Protected Analogue: Utilization by the Na: K Pump of Human Red Blood Cell Ghosts", Biochemistry, 1978, 17, 1929. 
2) E. Ohtsuka, H. Uemura, T. Doi, T. Miyake, S. Nishikawa and M. Ikehara, "A New Method for 3'Labelling of Polyribonucleotides by Phosphorylation with RNA Ligase and Its Application to the 3'-Modification for Joining Reactions", Nucleic Acids Res., 1979, 6, 443. 
3) J. A. McCray, L. Herbette, T. Kihara and D. R. Trentham, "A New Approach to Time-resolved Studies of ATP-requiring Biological Systems: Laser Flash Photolysis Caged ATP", Proc. Natl. Acad. Sci. USA, 1980, 77, 7237. 
4) Y. E. Goldman, M. G. Hibberd, J. A. McCray and D. R. Trentham, "Relaxation of Muscle Fibres by Photolysis of Caged ATP", Nature, 1982, 300, 701. 
5) D. H. Pierce, A. Scarpa, M. R. Topp and J. K. Blasie, "Kinetics of Calcium Uptake by Isolated Sarcoplasmic Reticulum Vesicles Using Flash Photolysis of Caged Adenosine 5'-Triphoshate", Biochemistry, 1983, 22, 5254. 
6) 杉晴夫, "アクチンとミオシンの構造と両者間の相互作用", 生体の科学, 1983, 34, 334. 
7) B. F. Iii, "Na⁺ Movement in a Single Turnover of the Na Pump", Proc. Natl. Acad. Sci. USA, 1984, 81, 5310. 
8) Y. E. Goldman, "Laser Pulsed Release of ATP and Other Optical Methods in the Study of Muscle Contraction", Soc. Gen. Physiol. Ser., 1986, 40, 397. 
9) J. W. Walker, G. P. Reid, J. A. McCray and D. R. Trentham, "Photolabile 1-(2-Nitrophenyl)ethyl Phosphate Esters of Adenine Nucleotide Analogues. Synthesis and Mechanism of Photolysis", J. Am. Chem. Soc., 1988, 110, 7170. 
10) J. W. Walker, G. P. Reid and D. R. Trentham, "[16] Synthesis and Properties of Caged Nucleotides", Methods in Enzymol., 1989, 172, 288. 
11) K. Horiuti, T. Sakoda, M. Takei and K. Yamada, "Effects of Ethylene Glycol on the Kinetics of Contraction on Flash Photolysis of Caged ATP in Rat Psoas Muscle Fibres", J. Muscle. Res. and Cell Motility, 1992, 13, 199. 
12) Z. Lu, R. L. Moss and J. W. Walker, "Tension Transients Initiated by Photogenerations of MgADP in Skinned Skeletal Muscle Fibers", J. Gen. Physiol., 1993, 101, 867.

SOD Assay Kit – WST

• 取扱説明書 500 tests 日本語
  
• 取扱説明書 500 tests English
  
• 取扱説明書 100 tests 日本語
  
• 取扱説明書 100 tests English
  
• プロトコル SOD様活性を測定したい
  

1) H. Ukeda, A. K. Sarker, D. Kawana and M. Sawamura, "Flow-Injection Assay of Superoxide Dismutase Based on the Reduction of Highly Water-Soluble Tetrazolium", Anal. Sci., 1999, 15, 353.
2) H. Ukeda, D. Kawana, S. Maeda and M. Sawamura, "Spectrophotometric Assay for Superoxide Dismutase Based on the Reduction of Highly Water-soluble Tetrazolium Salts by Xanthine-Xanthine Oxidase", Biosci. Biotechnol. Biochem., 1999, 63, 485.
3) <受田浩之, 森山洋憲, 川名大介, 片山泰幸, 中林錦一, 沢村正義, 「スーパーオキシドアニオン消去活性新規測定法の食品への応用」, 日本食品科学工学会誌, 2002,49, 25.
4) <森山洋憲, 片山泰幸, 中林錦一, 受田浩之, 沢村正義, 「高知県産茶および青果物の有するスーパーオキシドアニオン消去能の測定」, 日本食品科学工学会誌, 2002,49, 679.
5) H. Ukeda, T. Shimamura, M. Tsubouchi，Y. Harada, Y. Nakai and M. Sawamura, "Spectrophotometric Assay of Superoxide Anion Formed in Maillard Reaction Based on Highly Water-soluble Tetrazolium Salt", Anal. Sci., 2002, 18, 1151.
6) N. Tsuji, N. Hirayanagi, M. Okada, H. Miyasaka, K. Hirata, M. H. Zenk and K. Miyamoto, "Enhancement of Tolerance to Heavy Metals and Oxidative Stress in Dunaliella Tertiolecta by Zn-induced Phytochelatin Synthesis", Biochem. Biophys. Res. Commun., 2002, 293, 653.
7) A. Sakudo, D. C. Lee, K. Saeki, Y. Nakamura, K. Inoue, Y. Matsumoto, S. Itohara and T. Onodera, "Impairment of Superoxide Dismutase Activation by N-Terminally Truncated Prion Protein (PrP) in PrP-deficient Neuronal Cell Line", Biochem. Biophys. Res. Commun., 2003, 308, 660.
8) J.-H. Zhu, X. Zhang, J. P. Mcclung and X. G. Lei, "Impact of Cu,Zn-Superoxide Dismutase and Se-Dependent Glutathione Peroxidase-1 Knockouts on Acetaminophen-Induced Cell Death and Related Signaling in Murine Liver", Exp. Biol. Med., 2006, 231, 1726.
9) H. R. Rezvani, S. Dedieu, S. North, F. Belloc, R. Rossignol, T. Letellier, H. de Verneuil1, A. Taieb1, F.Mazurier, "HIF-1α: a Key Ffactor in the Keratinocyte Response to UVB Exposure", J. Biol. Chem., 2007, 10, 1074.
10) S. Goldstein and G. Czapski, "Comparison Between Different Assays for Superoxide Dismutase-like Activity", Free Rad. Res. Comms., 1991, 12, 5.
11) R. H. Burdon, V. Gill and C. Rice-Evans, "Reduction of a Tetrazolium Salt and Superoxide Generation in Human Tumor Cells (HeLa)", Free Rad. Res. Commun., 1993,18, 369.
12) Y. Sakurai, I. Anzai and Y. Furukawa, "A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide Dismutase", J. Biol. Chem., 2014, 289(29), 20139.
13) A. Weichert, A. S. Besemer, M. Liebl, N. Hellmann, I. Koziollek-Drechsler, P. Ip, H. Decker, J. Robertson, A. Chakrabartty, C. Behl and A. M. Clement, "Wild-type Cu/Zn superoxide dismutase stabilizes mutant variants by heterodimerization", Neurobiol. Dis., 2014, 62, 479.
14) J. M. Hartney, T. Stidham, D. A. Goldstrohm, R. E. Oberley-Deegan, M. R. Weaver, Z. Valnickova-Hansen, C. Scavenius, R. K.P. Benninger, K. F. Leahy, R. Johnson, F. Gally, B. Kosmider, A. K. Zimmermann, J. J. Enghild, E. Nozik-Grayck and R. P. Bowler, "A common polymorphism in extracellular superoxide dismutase affects cardiopulmonary disease risk by altering protein distribution", Circ Cardiovasc Genet, 2014, 7(5), 659.
15) J. Wu, Y. Sun, Y. Zhao, J. Zhang, L. Luo, M. Li, J. Wang, H. Yu, G. Liu, L. Yang, G. Xiong, J. Zhou, J. Zuo, Y. Wang and J. Li, "Deficient plastidic fatty acid synthesis triggers cell death by modulating mitochondrial reactive oxygen species", Cell Res., 2015, 25(5), 621.
16) E. Harunari, C. Imada, Y. Igarashi, "Konamycins A and B and Rubromycins CA1 and CA2, Aromatic Polyketides from the Tunicate-Derived Streptomyces hyaluromycini MB-PO13^T'", J. Nat. Prod., 2019, 82, (6), 1609-1615.
17) N. Kajihara, D. Kukidome, K. Sada, H. Motoshima, N. Furukawa, T. Matsumura, T. Nishikawa and E. Araki, "Low glucose induces mitochondrial reactive oxygen species via fatty acid oxidation in bovine aortic endothelial cells", J Diabetes Investig, 2017, 8, (6), 750.
18) H- J. Kim, H- J. Kim, J. Jeon, K- C. Nam, K- S. Shim, J- H. Jung, K. S. Kim, Y. Choi, S- H. Kim, A. Jang, Comparison of the quality characteristics of chicken breast meat from conventional and animal welfare farms under refrigerated storage', Poultry Science., 2020, 99, 1788-1796.
19) T. Shoji, S. Masumoto, N. Moriichi, Y. Ohtake, T. Kanda, Administration of Apple Polyphenol Supplements for Skin Conditions in Healthy Women: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial', Nutrients., 2020, 12, (1071), .
20) Y. Hirata, Y. Ito, M. Takashima, K Yagy, K. Oh-Hashi, H. Suzuki, K. Ono, K. Furuta and M. Sawada, Novel Oxindole-Curcumin Hybrid Compound for Antioxidative Stress and Neuroprotection.', ACS Chem. Neurosci.., 2020, 11, (1), 76-85.

–SulfoBiotics– Sodium sulfide (Na₂S)

• 取扱説明書 日本語
  
• 取扱説明書 English
  
• プロトコル
  

1) R. Greiner, Z. Palinkas, K. Basell, D. Becher, H. Antelmann, P. Nagy and T. P. Dick, “Polysulfides link H₂S to protein thiol oxidation”, Antioxid. Redox Signal., 2013, 19, 1749.
2) N. S. Lawrence, J. Davis and R. G. Compton, “Analytical strategies for the detection of sulfide: a review”, Talanta, 2000, 52, 771
3)Y.Kawahara, Y.Hirashita, C.Tamura, Y.Kudo, K.Sakai, K.Togo, K.Fukuda, O.Matsunari, K.Okamoto, R.Ogawa, K.Mizukami, T.Okimoto, M.Kodama, K.Murakami, "Helicobacter pylori infection modulates endogenous hydrogen sulfide production in gastric cancer AGS cells", Helicobacter, 2020,  https://doi.org/10.1111/hel.12732.

4NA(EDTA・4Na)

DiBAC₄(3)

• プロトコル 膜電位を測定したい
  

1) D. E. Epps, M. L. Wolfe and V. Groppi, "Characterization of the Steady-state and Dynamic Fluorescence Properties of the Potential-sensitive Dye Bis-(1,3-dibutylbarbituric acid)trimethine oxonol(Dibac4(3)) in Model Systems and Cells", Chem. Phys. Lipids, 1994, 69, 137.
2) T. Braner, D. F. Hulser and R. J. Strasser, "Comparative Measurements of Membrane Potentials with Microelectrodes and Voltage-sensitibe Dyes", Biochim. Biophys. Acta, 1984, 771, 208.
3) T. T. Rohn, A. Sauvadet, C. Pavoine and F. Pecker, "Xanthine Affects [Ca²⁺]_i and Contractile Responses of Ventricular Cardiocytes to Electrical Stimulation", Am. J. Physiol., 1997, 273, C909.
4) D. J. Mason, R. Allman, J. M. Stark and D. Lloyd, "Rapid Estimation of Bacterial Antibiotic Susceptibility with Flow Cytometry", J. Microsc., 1994, 176, 8.
5) U. Langheinrich and J. Daut, "Hyperpolarization of Isolated Capillaries from Guinea-pig Heart Induced by K⁺ Channel Openers and Glucose Deprivation", J. Physiol., 1997, 502, 397.
6) V. Dall'Asta, R. Getti, G. Orlandini, P. A. Rossi, B. M. Rotoli, R. Sala, O. Bussolati and G. C. Gazzola, "Membrane Potential Changes Visualized in Complete Growth Media through Confocal Laser Scanning Microscopy of bis-Oxonol-loaded Cells", Exp. Cell Res., 1997, 231, 260.
7) K. S. Schroeder and B. D. Neagle, "FLIPR: A New Instrument for Accurate, High Throughput Optical Screening", J. Biomol. Screening, 1996, 1, 75.

ECGreen-Endocytosis Detection

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• 取扱説明書 English
  

2NH₄(EDTA・2NH₄)

–SulfoBiotics– Sodium disulfide (Na₂S₂)

Sodium polysulfideの構造のドナー3種 （ Sodium disulfide　Na₂S₂、Na₂S₃、Na₂S₄ )
のセットをご用意しました。

• 取扱説明書 日本語
  
• 取扱説明書 English
  
• プロトコル サルフェン硫黄を系の中に加えたい
  

1) Y. Kimura, Y. Mikami, K. Osumi, M. Tsugane, J. Oka, and H. Kimura, "Polysulfides are possible H₂S-derived signaling molecules in rat brain", FASEB J., 2013, 27, 2451.
2) S. Koike, Y. Ogasawara, N. Shibuya, H. Kimura, and K. Ishii, "Polysulfide exerts a protective effect against cytotoxicity caused by t-buthylhydroperoxide through Nrf2 signaling in neuroblastoma cells", FEBS Lett., 2013, 587, 3548.
3) E. R. DeLeon, Y. Gao, E. Huang, M. Arif, N. Arora, A. Divietro, S. Patel, and K. R. Olson , "A case of mistaken identity: are reactive oxygen species actually reactive sulfide species?", American Journal of Physiology: Regulatory, Integrative and Comparative Physiology ., 2015, 310, (7), 549.
4) D. Delgermuruna, S. Yamaguchia, O. Ichiib, Y. Konb, S. Itoa, and K. Otsuguroa, "Hydrogen sulfide activates TRPA1 and releases 5-HT from epithelioid cells of the chicken thoracic aorta", Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology., 2016, 187, 43 .
5) S. Koikea, T. Kayamaa, S. Yamamotoa, D. Kominea, R. Tanakaa, S. Nishimotoa, T. Suzukia, A. Kishidab, and Y. Ogasawaraa, "Polysulfides protect SH-SY5Y cells from methylglyoxal-induced toxicity by suppressing protein carbonylation: A possible physiological scavenger for carbonyl stress in the brain", NeuroToxicology., 2016,55, 13 .