人多能性干细胞无血清培养基


产品编号 产品名称 产品规格 产品等级 产品价格
197-17571 StemSure® hPSC MediumΔ 100ml 用于细胞培养
193-17573 StemSure® hPSC MediumΔ 100ml×4 用于细胞培养
064-05381  Fibroblast Growth Factor (basic), Human, recombinant, Animal-derived-free【bFGF/FGF2】 50μg 用于细胞培养
068-05384 Fibroblast Growth Factor (basic), Human, recombinant, Animal-derived-free【bFGF/FGF2】 100μg 用于细胞培养
060-05383 Fibroblast Growth Factor (basic), Human, recombinant, Animal-derived-free【bFGF/FGF2】 1mg 用于细胞培养
257-00511 Y-27632 1mg 用于细胞培养
253-00513 Y-27632 5mg 用于细胞培养
251-00514 Y-27632 25mg 用于细胞培养
253-00591 5mmol/l Y-27632 Solution 300μl 用于细胞培养
220-02041 Vitronectin(20-398 aa), Human, recombinant,Solution 500μg 生物化学用
197-16275 StemSure® D-MEM(High Glucose)with Phenol Red and Sodium Pyruvate 500ml 用于细胞培养
197-16775 StemSure® Serum Replacement 500ml 用于细胞培养
198-15781 StemSure® 10mmol/l 2-Mercaptoethanol Solution (×100) 100ml 用于细胞培养
195-15791 StemSure® 50mmol/l Monothioglycerol Solution (×100) 100ml 用于细胞培养
190-15805 StemSure® 0.1w/v% Gelatin Solution 500ml 用于细胞培养
199-16051 StemSure® LIF, Mouse, recombinant, Solution 106units 用于细胞培养
195-16053 StemSure® LIF, Mouse, recombinant, Solution 106units×10 用于细胞培养
195-16031 StemSure® Freezing Medium 100ml 用于细胞培养

StemSure® hPSC培养基Δ人多能性干细胞无血清培养基

人多能性干细胞无血清培养基


人多能性干细胞无血清培养基

 

  本产品是无饲养层条件下用于维持培养人ES细胞·iPS细胞的无血清,无动物源性成分的液体培养基。不含BSA及HAS等蛋白,批次间的差别较少,结果稳定。但是,本产品不含bFGF(碱性線成纤维细胞成长因子)。


◆特点


  ●无血清、无饲养层培养人ES· iPS细胞

  ●使用人iPS细胞201B7细胞系,进行每批次的质量检测

  ●不含动物源成分

  ●不属于医药用外毒物或剧毒物,保存及处理简单

  ●不含蛋白的低蛋白培养基

  ●可使用Matrigel® 、iMatrix-511、Vitronectin等培养基质

  ●可使用Accutase, TrypLE Select等解离液

  ●传代培养时添加Y-27632可传代培养单细胞


◆检测项目


  ●细胞繁殖能力测试(使用人iPS细胞201B7细胞系)

  ●碱性磷酸酶染色(使用人iPS细胞201B7细胞系)

  ●无菌检测

  ●pH

  ●渗透压

  ●内毒素

  ●支原体检测


使用注意

 

  ●本产品不含bFGF(碱性成纤维细胞成长因子)。

  ●本产品需冷藏(-20℃)。溶解后无需再冷冻,请保存于2~10℃,并在1周内使用完毕。

 

人iPS细胞201B7细胞系


  将使用A公司培养基(含有BSA、使用动物源成分)培养的人iPS细胞201B7细胞系移植到StemSure® hPSC培养基Δ,进行传代培养。下图为传代移植培养时的细胞形态及细胞集団倍增数、未分化性标记表达结果。



《 细胞形态 》

  使用StemSure® hPSC培养基Δ培养的人iPS细胞的形态与A公司培养基的几乎相同,没有发生分化,稳定地进行了培养。


人多能性干细胞无血清培养基

《 细胞群倍加数 》

  对比移植到StemSure® hPSC培养基Δ后的第1代到第5代的细胞,使用StemSure® hPSC培养基Δ的细胞繁殖状况更好。

 < 培养基成分 > 
  StemSure® hPSC培养基Δ + 35ng/ml bFGF

< 细胞播种数 > 
  1×105cells/well(使用6孔培养板)

 

 


《 未分化性标记表达确认 》

  使用StemSure® hPSC培养基Δ培养至5代,观察到全部的未分化标记物(Nanog, Oct3/4, Tra-1-60, SSEA-4, BC2LCN)。  

   ※BC2LCN是能与存在于人ES ・iPS细胞膜表面的糖链特异性结合的重组凝集素。

人多能性干细胞无血清培养基

< 培养基成分 > 
  StemSure® hPSC培养基Δ + 35ng/ml bFGF

< 细胞播种数 > 
  1×105cells/well(使用6孔培养板)

 

 

《 未分化性标记表达确认 》

  使用StemSure® hPSC培养基Δ培养至5代,观察到全部的未分化标记物(Nanog, Oct3/4, Tra-1-60, SSEA-4, BC2LCN)。  

   ※BC2LCN是能与存在于人ES ・iPS细胞膜表面的糖链特异性结合的重组凝集素。

人多能性干细胞无血清培养基

<数据提供 独立行政法人产业技术综合研究所 干细胞工学研究中心 小沼泰子老师、伊藤弓弦老师>


人ES细胞201B7细胞系


  将使用A公司培养基(含有BSA、使用动物源成分)培养的人ES细胞201B7细胞系移植到StemSure® hPSC培养基Δ,进行传代培养。下图为传代移植培养时的细胞形态及细胞集団倍增数、未分化性标记表达结果。

《 细胞形态 》

  使用A公司培养基的细胞,细胞在第2代以后出现了分化。另一方面,使用StemSure® hPSC培养基Δ的细胞,没有发生分化,稳定地进行了培养。

 

人多能性干细胞无血清培养基

《 细胞群倍增数 》


  比较两者移植到StemSure® hPSC培养基Δ后的第1代到第5代,细胞的繁殖能力没有差异。


人多能性干细胞无血清培养基

< 培养基成分 > 
  StemSure® hPSC培养基Δ + 35ng/ml bFGF

< 细胞播种数 > 
  1×105cells/well(使用6孔培养板)

 



《 未分化性维持確認 》


  使用StemSure® hPSC培养基Δ培养至第6代,观察到全部的未分化标记物(Nanog, Oct3/4, Tra-1-60, SSEA-4, BC2LCN)。   

   ※BC2LCN是能与存在于人ES ・iPS细胞膜表面的糖链特异性结合的重组凝集素。


人多能性干细胞无血清培养基

<数据提供  独立行政法人产业技术综合研究所 干细胞工学研究中心 小沼泰子老师、伊藤 弓弦老师>

 

 

核型解析


  对使用StemSure® hPSC培养基Δ培养到第27代的人iPS细胞进行了核型解析,没有发现染色体异常。 


人多能性干细胞无血清培养基

<数据提供  独立行政法人国立成育医疗研究中心研究所 再生医疗中心 三浦巧老师、阿久津英憲老师>

 

 

人iPS细胞201B7分化成三胚层


  使用StemSure® hPSC培养基Δ将人iPS细胞201B7细胞系培养至第3代,再使用StemSure® 系列培养基进行驯化培养,可观察到βⅢ-Tubulin、α-SMA、AFP,因此判断细胞形成胚体后,进行了三胚层分化。


人多能性干细胞无血清培养基

< 培养基成分 >
  StemSure® D-MEM + StemSure® Serum Replacement + 2mmol/l L-Glutamine +  10mmol/l StemSure® 2-Mercaptoetanol + 1 x Non-essential Amino Acids Solution   

 

Grand PFA烧杯 (可附手柄)


产品编号 产品名称 产品规格 产品等级 产品价格
11110 Grand PFA烧杯 (附手柄) 100mL
11111 Grand PFA烧杯 (附手柄) 200mL
11112 Grand PFA烧杯 (附手柄) 500mL
11113 Grand PFA烧杯 (附手柄) 1L
11114 Grand PFA烧杯 (附手柄) 2L
11115 Grand PFA烧杯  30mL
11116 Grand PFA烧杯 50mL
11117 Grand PFA烧杯  100mL
11118 Grand PFA烧杯  200mL
11119 Grand PFA烧杯 300mL
11120 Grand PFA烧杯 500mL
11121 Grand PFA烧杯 1L

Grand PFA烧杯 (可附手柄)


耐化学性、耐热性优异,不含金属的氟树脂制烧杯。

溶液和试剂都不易沾着,附手柄,使用便利。可使用磁力搅拌器搅拌。

Grand PFA烧杯 (可附手柄)

   

◆原理

 

      三博特独创品牌Grand PFA烧杯使用高品质「NEW PFA」制成的烧杯。配备30ml~2000ml 规格的产品。并可根据用途选择有手柄或没手柄的产品。旧PFA和NEW PFA的溶出氟离子比较,PFA 氟树脂的耐化学性和耐热性优异,常被用于半导体工艺里的晶圆制造及管道系统。Grand PFA烧杯的原材料——NEW PFA为LSI高密度、高集成后生成的周边材料,对应高纯度需要的高品质PFA。

Grand PFA烧杯 (可附手柄)

Grand PFA烧杯 (可附手柄)



◆优点特色

 

耐化学性、耐热性、耐寒性优越,酸、碱、有机溶剂等大部分液体都可使用。可直接放冰箱内冷藏或油浴加热。
无金属溶出不必担心样品被微量金属污染,适合微量分析。
不粘附样品,即使样品粘度高也很容易倒出无需特殊操作。从一般的试料到粘度高的试料都可以使用。
透明度超群。使用三博特独自研制的模具,Grand PFA 烧杯透明度比旧产品更出众
一体成型的手柄,以前的手柄由PTFE制,不能与烧杯溶为一体,新产品整个烧杯一体成形无需担心手柄裂开。设计适合人手抓拿,即使烧杯装满液体也不会握不住。
平坦的底面,杯底平滑,可放于涡旋搅拌器上使用。
鲜明的刻度,刻度突起,即使手湿也不怕打滑,同时刻度比旧产品更大,方便观察。
批次控制,全部产品的底面都有材料标识、生产日期等刻印,方便使用。
耐候性,很少受到紫外线的影响,很少变黄,变质,可长期维持好的品质。


Grand PFA烧杯 (可附手柄)

◆案例应用

适合微量分析。
高精度分析、半导体业界里使用、同时提供经过酸洗可抑制金属溶出的Clean package。


Grand PFA烧杯 (可附手柄)

三博特容器类产品单页

Q:    「铁氟龙烧杯」与「PFA烧杯」是由不同材料制造的吗?
A:    一般所说的「铁氟龙」就是「氟树脂」,但实际上「铁氟龙」是杜邦公司注册的商标名称。严格来说,

          「氟树脂」是合成树脂其中的一个分类,而「铁氟龙」是生产商使用的产品名。
          三博特(sanplatec)采用大金工业株式会社的「PFA」原料,与「铁氟龙」同样都为氟树脂。
    
Q:    PFA是什么?是氟树脂的一种吗?
A:    氟树脂是分子结构中含有氟原子的一类热塑性树脂。具有优异的耐高低温性能、介电性能、化学稳定性、

          耐候性、不燃性、不粘性和低的摩擦系数等特性。PFA是其中一种氟树脂,拥有优异的耐热性、耐药性、

          电学特性(高频率特性)、不粘着、自润性。
    
Q:    我现在正在使用PTFE铁氟龙烧杯。
          GrandPFA烧杯透明度比PTFE铁氟龙烧杯好,耐药性也可以跟PTFE相比吗?
A:    PTFE和PFA都是氟树脂的一种。
          两种材料都拥有几乎相同的耐药性和耐热性。而PFA的透明度比PTFE更好,更方便顾客使用。


N465 AGAROSE BROAD RANGE TRIAL KIT AMRESCO N465-KIT

名称:N465 AGAROSE BROAD RANGE TRIAL KIT

品牌:AMRESCO

订货号:N465-KIT

N465 AGAROSE BROAD RANGE TRIAL KIT                                                        AMRESCO                                                        N465-KIT

咨询此产品

产品介绍

N465
AGAROSE BROAD RANGE TRIAL KIT

Storage Condition:
ROOM TEMPERATURE

AMRESCO agaroses are available in easy-to-use trial kits that provide convenient options for testing a variety of agaroses for specific applications.Each kit contains 3g of samples of Agarose I, Agarose LF and Agarose SFR.  Kit is also supplied with 5X Loading Buffer, TBE Buffer, 10X Ready-Pack and TAE Buffer, 25X Ready-Pack.

Cosmo GlyScope Series标签试剂盒


产品编号 产品名称 产品规格 产品等级 产品价格
JCM-J710 GlyScope ABEE标签试剂盒
 GlyScope ABEE Labeling Kit
1 kit

Cosmo GlyScope Series标签试剂盒GlyScope系列产品

适用于糖链研究

 

背景

  众所周知,糖链是通过结合蛋白(糖蛋白)和脂质(糖脂)在细胞中发挥功能。糖蛋白和糖脂已被证明在体内扮演多种多样的角色。


 Cosmo GlyScope Series标签试剂盒

 

◆特点和优点

● 适用于实验室通用仪器

● 快速分析系统

● 可用于高效液相色谱法分析糖类 

 

MBPTrap HP 预装柱,28-9187-78

MBPTrap HP 预装柱,28-9187-78

 

MBPTrap HP 预装柱

品牌: GE Healthcare Life Sciences
规格: MBPTrap HP (5x1ml)
型号: 28-9187-78
产品简介
装有1ml和5mlDextrin Sepharose HP凝胶的预装柱;一步纯化即得高纯度;高纯度的蛋白洗脱呈现窄峰;洗脱条件温和,保护目标蛋白的活性;耐NaOH的清洗;可方便的配合注射

Wako SeeDB实现生物体样本深层成像


产品编号 产品名称 产品规格 产品等级 产品价格
291-79601 SeeDB实验试剂盒
 SeeDB Trial Kit
1kit 组织透明化
193-18391 SeeDB:20w/v% 果糖溶液
 SeeDB:20w/v% Fructose Solution
250mL 组织透明化
196-18401 SeeDB:40w/v% 果糖溶液
 SeeDB:40w/v% Fructose Solution
250mL 组织透明化
193-18411 SeeDB:60w/v% 果糖溶液
 SeeDB:60w/v% Fructose Solution
250mL 组织透明化
190-18421 SeeDB:80w/v% 果糖溶液
 SeeDB:80w/v% Fructose Solution
250mL 组织透明化
197-18431 SeeDB:100w/v% 果糖溶液
 SeeDB:100w/v% Fructose Solution
250mL 组织透明化
194-18441 深层透明化试剂
 SeeDB
250mL 组织透明化

Wako SeeDB实现生物体样本深层成像SeeDB

实现生物体样本深层成像

  今井猛博士等人开发了一种对于水溶性生物体组织的形态和组成在不破坏荧光蛋白以及荧光神经同位素的条件下,可快捷简便地使大脑等生物体组织透明化的方法——SeeDB(See Deep Brain)。SeeDB由水、果糖、还原剂构成。

  SeeDB法是在使用共聚焦显微镜和双光子激发显微镜下可深层成像的方法。可利用荧光蛋白和神经示踪剂,实现对荧光神经回路的全貌阐明和定量分析等各种各样的应用。


详细内容,请点击SeeDB技术开发者今井猛博士的网站:SeeDB Resources(本部分内容亦可参见相关资料

◆SeeDB protocol案例


  SeeDB法,可用于脊椎动物的各种组织,下面以小鼠大脑为例进行介绍。


1.固定

 将小鼠大脑在4%多聚甲醛/PBS,4℃下固定过夜。

 样本用PBS清洗三次(每次清洗10分钟)


2.透明化处理

③ 将样品加入放有20 mL的SeeDB:20 w/v%果糖溶液的50 mL锥形瓶中。

  在室温下在旋转器中旋转4-8小时(约4 rpm)

  在脆弱的样本和薄片情况也可以用压板式摇床(约17 rpm)

④ 将样本加入放有SeeDB:40 w/v%果糖溶液的50mL锥形管中,室温下旋转4-8小时。

⑤ 将样本加入放有SeeDB:60 w/v%果糖溶液的50mL锥形管中,室温下旋转4-8小时。

⑥ 将样本加入放有SeeDB:80 w/v%果糖溶液的50mL锥形管中,室温下旋转12小时。

⑦ 将样本加入放有SeeDB:100 w/v%果糖溶液的50mL锥形管中,室温下旋转12小时。

⑧ 将样本加入放有SeeDB的50 mL锥形管中,室温下旋转24小时。

    最长时间可延长至48小时。在透明化成功时,透光可观察到组织透明化。


3.观察

⑨ 将SeeDB处理过的大脑样本置于共聚焦显微镜或双光子激发显微镜下观察。


(注意点)

  样本用SeeDB液固定。用SeeDB透明化处理后的样本折射率可达到1.49。因此,物镜中应该有甘油浸没透镜、油镜、透明化用镜片,即使浸水镜头到达2 mm深度也是没有问题的。浸没式镜片请使用浸没液,SeeDB不能用作浸没液。在使用干式镜头(折射率1.0)和浸水镜头(折射率1.33)获取画像情况下,需要补正深度值(补正值请参考相关文献)。

 


SeeDB 处理案例

Wako SeeDB实现生物体样本深层成像

SeeDB生物体样本透明化

左起依次为经SeeDB液透明化处理的小鼠幼胎(幼胎12天)

新生鼠(生后3天)的全脑、成鼠大脑(8周龄,厚度2 mm)


SeeDB 观察案例

Wako SeeDB实现生物体样本深层成像

Wako SeeDB实现生物体样本深层成像

双光子激发显微镜下的Thy1-YFP-H小鼠

(10周龄)大脑荧光成像

使用Olympus公司产品 

多光子专用物镜:XLPLN10XSVMP观察案例

共聚焦显微镜下的Thy1-YFP-H小鼠

(10周龄)大脑荧光成像

使用Olympus公司产品

有机硅浸没式物镜:UPLSAPO 10X2观察案例

Wako SeeDB实现生物体样本深层成像

双光子激发显微镜下的Thy1-YFP-H小鼠

(10周龄)大脑荧光成像

使用Olimpus公司产品

多光子专用物镜:XLSLPLN25XGMP观察案例

比例尺:100 μm

Wako SeeDB实现生物体样本深层成像

小鼠大脑固定后用Dil标记,SeeDB透明化处理案例   

左:嗅球僧帽细胞树突(横向观察)

右:嗅球僧帽细胞树突(纵向观察)

使用Olympus公司产品

有机硅浸没型物镜:UPLSAPO 20X观察案例。比例尺:100 μm。


组织透明化配套耗材:成像用透明室

Wako SeeDB实现生物体样本深层成像

透明化试剂方法选择流程.pdf

组织透明化试剂Q&A ver1.0

Tissue clearing reagent Q&A ver1.0

Q组织透明化的原则

A:组织透明化通常是按下面的步骤进行的:

A:(a)固定 (b)透化 (c)脱色 (d)折射率匹配

 

Q:组织透明化的优势

A:目前大多数可用的透明化技术,都是通过编码荧光报告蛋白或者用荧光标记抗体进行后标记,从而可视化

A:组织中特定的蛋白。组织透明化成为了具有单细胞分辨率的组织3D成像中重要的一环。

 

Q:透明化技术的比较

A:下面是水性的透明化技术。

Wako SeeDB实现生物体样本深层成像


Wako SeeDB实现生物体样本深层成像

A:SeeDB(基于果糖的方法)在一些应用中有一定优势。因为SeeDB不含去垢剂,所以对于透明化DiI(细胞膜

A:红色荧光探针,一种神经示踪剂)标记的样品和使用光电关联显微镜(CLEM)的情况下,SeeDB有着一定优

A:势。另外SeeDB会产生自体荧光,有时候会对辨认未标记的神经元结构(如神经毡)起着一定帮助。

A:SeeDB2是为使用高NA物镜的高分辨率成像而优化的技术;因此,当处理较大的组织时,CLARITY或者CUBIC

A:会是更好的选择。

 

Q:切片厚度的最大值和最小值?

A:您应该根据您的物镜的WD(工作距离)来决定切片的厚度。我们建议切片的厚度不要超过3mm。对于十分薄

A:且脆弱的样品,我们建议使用琼脂糖包埋。

A:使用CUBIC,可以让全器官、全身透明化和使用LSFM的快速3D成像具有可重复性。

 

Q:试剂盒成分

A:具体如下:

A:1. SCALEVIEW-S试剂盒(299-79001)适用于20份1-2mm的切片样品

A:A:1) SCALEVIEW-S0——100mL

A:A:2) SCALEVIEW-S1——100mL

A:A:3) SCALEVIEW-S2——100mL

A:A:4) SCALEVIEW-S3——100mL

A:A:5) SCALEVIEW-S4——100mL

A:A:6) SCALEVIEW-SMT——100mL

 

A:2. SCALEVIEW-S试剂盒(290-80801)适用于20-30份1-2mm的切片样品。

A:A:1) SCaleCUBIC-1 Solution——250mL

A:A:2) SCaleCUBIC-2 Solution——250mL

A:A:3) Mounting Solution 1——40mL

A:A:4) Mounting Solution 2——40mL

 

A:3. SeeDB试剂盒(299-79901)适用于20-30份1-2mm的切片样品。

A:A:1) SeeDB:20w/v% Fructose Solution——50mL

A:A:2) SeeDB:40w/v% Fructose Solution——50mL

A:A:3) SeeDB:60w/v% Fructose Solution——50mL

A:A:4) SeeDB:80w/v% Fructose Solution——50mL

A:A:5) SeeDB:100w/v% Fructose Solution——50mL

A:A:6) SeeDB——50mL

 

A:4. SeeDB2试剂盒(294-80701)适用于20-30份1-2mm的切片样品。

A:A:1) Saponin(皂角苷)——5g

A:A:2) SeeDB2G solution——50mL

A:A:3) SeeDB2S solution——50mL

A:A:4) PBS——50mL

 

Q:样品的保存

A:SCALEVIEW-S处理的样品应在室温下保存2至3周。您可以在2至3周内观察SCALEVIEW-S透明化的样品中的

A:荧光信号。

A:CUBIC处理的样品应在室温下保存1周。您可以在1周内观察CUBIC透明化的样品中的荧光信号。

A:SeeDB2G处理的样品应在冰箱中保存1年,而SeeDB2S处理的样品则可在室温下保存1年。您可以在1年内观

A:察SeeDB透明化的样品中的荧光信号。

 

Q:抗体的荧光标记

A:应在进行透明化前就进行抗体的荧光标记。

 

Q:适用于SCALEVIEW-S的显微镜

A:SD-OSR(Olympus)

A:FV-OSR(Olympus)

 

Q:适用于CUBIC的显微镜

A:SD-OSR(Olympus)

A:FV-OSR(Olympus)

A:Zeiss Lightsheet Z.1(Carl Zeiss)

 

Q:适用于SeeDB2的显微镜

A:Confocal(最好使用高NA的油浸镜头)

A:STED(Leica Microsystems)

A:SR-SIM(Carl Zeiss)

A:SD-OSR(Olympus)

A:FV-OSR(Olympus)

A:Airyscan(Carl Zeiss)

A:HyVolution(Leica Microsystems)

A:Two-photon(最好使用复合浸没镜头)

A:Confocal(最好使用高NA的甘油浸镜头)

A:Light-sheet DLS(Leica,最好使用定制镜面的设备)

 

Q:重构

A:对于定量用途,我们建议使用Neurolucida系统(MBF)。我们同样测试了VAST Lite用于成像用途时的表

A:现。重构时也可以使用开源软件。例如,Vaa3d可以处理大量的拼接图像的数据。

 

Q:物镜推荐

Wako SeeDB实现生物体样本深层成像

 

Q:其他物种?其他器官?

A:对于昆虫样品和鱼类样品,建议选择CUBIC。

A:昆虫相关文献:Ohuma.et al.: Fish Pathology, 52(2), 96(2017).

A:鱼类相关文献:Konno and S.Okazaki .: Zoological Letters, 4:13(2018).

   

  对于多细胞球状体,选择SCALEVIEW-S会更好。

  https://www.nature.com/articles/s41598-018-29169-0

 

参考文献:

[1] Ke, M. T., Fujimoto, S. and Imai, T. : Nat Neurosci ,6 (8),1154 (2013).

[2] Ke, M. T., Fujimoto, S. and Imai, T.: Bio-protocol ,4(3), e1042 (2014).

[3] Ke, M. T., and Imai, T. : Curr Protoc Neurosci ,66, 2.22.1-2.22.19 (2014).

乙酸[AK法]检测试剂盒 Acetic Acid (AK; analyser format) 货号:K-ACETAK Megazyme中文站

乙酸[AK法]检测试剂盒

英文名:Acetic Acid (AK; analyser format)

货号:K-ACETAK

规格:170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL)

分析物意义: 常见食品的组分

Megazyme检测试剂盒优点: K-ACETRM 是运用AK和磷酸乙酰转移酶的新型、快速的手工检测试剂盒。试剂稳定

K-ACETAK (自动) 是一种以乙酸激酶(AK)为基础的,新型、稳定、快速的检测试剂盒,具有良好的线性。

 

Analyser format for the specific assay of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution. Content:170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL)

Analyser format UV-method for the determination of Acetic Acid
in foodstuffs, beverages and other materials

Principle:
(acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(D-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → D-lactic acid + NAD+

Kit size: 550 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 10 min
Detection limit: 10 mg/L (recommended assay format)
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables,
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products
(and baking agents), ketchup, soy sauce, mayonnaise, dressings,
paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions),
feed and other materials (e.g. biological cultures, samples, etc.)
Method recognition: Improved method

Advantages

  • Very stable reagent when prepared for auto-analyser applications (> 7 days at 4°C)
  • PVP incorporated to prevent tannin inhibition
  • Linear calibration (R2 ~ 0.9995) up to 30 μg/mL of acetic acid in final reaction solution
  • Validated by the University of Wine, Suze la Rousse, France
  • Very rapid reaction
  • Very competitive price (cost per mL of reagent)
  • All reagents stable for > 2 years

  • Extended cofactors stability

 

1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and   therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

3. Does the decolourising preparation remove some VA during the process?

No, however the sample preparation process can be tested by adding a known amount of acetic acid standard and assessing the recovery of this. 

4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

5. What are the major the differences between the various acetic acid test kits?

Megazyme produces 4 acetic acid test kits:
K-ACET: uses the traditional ACS reaction.  Manual format for use with spectrophotometers.
K-ACETAF: uses the traditional ACS reaction.  Automated format for use with auto-analysers.
K-ACETAK: uses the more recently developed and more rapid acetate kinase reaction.  Automated format for use with auto-analysers.
K-ACETRM: uses the more recently developed and more rapid acetate kinase reaction.  Manual format for use with spectrophotometers. 

6. Can acetic acid be measured in culture/fermentation media?

Acetic acid in liquid cell culture media/supernatants or fermentation samples can be determined without any sample treatment (except clarification by centrifugation or filtration) and appropriate dilution in distilled water. 

7. Which acetic acid kit is recommended for a 96-well microplate format?

Auto-analysers use ~ 0.315 mL reaction volumes and pathlengths between 4-8 mm which is similar to a standard 96-well microplate where a 0.315 mL reaction volume would give a pathlength of ~ 6-7 mm.  Therefore K-ACETAK or K-ACETAF can be used directly in a 96-well microplate format with minimal assay optimisation.
If preferred, K-ACET or K-ACETRM may also be easily converted for use in a 96-well microplate format.  Basically, the assay volumes for the cuvette format must be reduced approximately 10-fold for use in a 96-well microplate.  However, some assay optimisation may be required (e.g. increased enzyme concentration etc.) and unlike the cuvette which has a set pathlength of 1 cm, the pathlength in the microplate is dependent upon the volume of liquid in the well.  Therefore to enable the calculation of the amount of analyte in the samples from tests performed in the microplate format one of the following must be done:

  1. The easiest method is to use a microplate reader that has a pathlength conversion capability (i.e. the microplate reader can detect the pathlength of each well and convert the individual readings to a 1 cm pathlength).  This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm pathlength) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values.


Acetic Acid Kit Recommendation For Microplate Format:
Either K-ACETRM or K-ACETAK is recommended for use in a 96-well microplate format and the main advantages / disadvantages are described below:
K-ACETRM:
The assay volumes of this kit should be reduced by 10-fold for use in a 96-well microplate format (some assay optimisation may be required, e.g. increased enzyme concentration etc.).
The calculation of results is achieved as outlined above in either of points 1, 2 or 3. 

7. Is the acetic acid kit specific for acetate?

Propionate may react more slowly than acetate.

8. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

9. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

10. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

11. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q12. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q13. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q14. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefor

Hampton 蛋白结晶向导管Wizard Tube

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.

图片

Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.

图片

CRYO (I、II)
The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.

图片

Hampton PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Hampton PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Applications

  Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules

Features

Developed at Hampton Research
PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 – 9.2
PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 – 8.8) in the presence of monodisperse PEG 3,350
PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block
 Description

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules. The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.

PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.

PEG/Ion contains 48 unique reagents, 10 ml each.

PEG/Ion 2 contains 48 unique reagents, 10 ml each.

PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

MegaQuant™ 流量计加上D-果糖和D-葡萄糖试剂 MegaQuant™ Meter plus D-Fructose and D-Glucose Reagents 货号:D-FRGLMQ Megazyme中文站

MegaQuant™ 流量计加上D-果糖和D-葡萄糖试剂

英文名:MegaQuant™ Meter plus D-Fructose and D-Glucose Reagents

货号:D-FRGLMQ

规格:MegaQuant™ meter plus reagents for 60 D-fructose plus D-glucose determinations

The MegaQuant™ meter is used for D-glucose/D-fructose determinations as well as L-malic acid measurements. The contents of a D-glucose/D-fructose kit (60 determinations) is supplied with the meter.

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一次性医用口腔护理清洁海绵棒 病人老人婴幼儿口腔清洁棒 月子棒

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金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com

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原子吸光分析用金属离子标准液


产品编号 产品名称 产品规格 产品等级 产品价格

原子吸光分析用金属离子标准液

原子吸光分析用金属离子标准液

  金属元素共80多种,金属元素的研究重点集中在重金属的检测。现重金属污染已得到世界上各国政府、众多企业的关注。因此许多相关的法规应运而生,各国都制定了相关标准。因此,对于金属元素的定量检测是非常有必要的。目前,对于微量痕量金属进行检测的方法包括:原子吸光分析(AAS)、电感耦合等离子体原子发射光谱(ICP)、离子色谱(IC)法等。

  重金属指比重大于5的金属,原子量大于55的金属,约有45种,一般都是属于过渡元素。如铜、铅、锌、铁、钴、镍、锰、镉、汞、钨、钼、金、银等。尽管锰、铜、锌等重金属是生命活动所需要的微量元素,但是大部分重金属如汞、铅、镉等并非生命活动所必须,而且所有重金属超过一定浓度都对人体有毒。另外,砷虽不属于重金属,但因其来源以及危害都与重金属相似,故通常列入重金属类进行研究讨论。而且重金属污染具有累积性、食物链传递性和不易降解性。因此重金属污染是比有机物污染等污染更为严重的问题。

  以下为中国国标对于一些金属元素在食品、家具、染料等行业的一些限制标准。

 

◆水质相关的金属检测标准

标准

标准号

涉及到的金属离子

地下水质量标准

GB/T 14848-93

铁、锰、铜、锌、钼、钴、汞、砷、硒、镉、铬、铅、铍、钡、镍

再生铜、铝、铅、锌工业污染物排放标准

GB 31574-2015

铅、铜、锌、砷、镍、镉、铬、锑

生物制药行业污染物排放标准

DB 31/373-2010

汞、镉、铬、砷、硒

生活饮用水卫生标准

GB 5749-2006

砷、镉、铬、铅、汞、硒、铝、铁、锰、铜、锌、锑、钡、铍、硼、钼、镍、银、铊

石油化学工业污染物排放标准

GB 31571-2015

钒、铜、锌、铅、镉、砷、镍、汞、铬

地表水环境质量标准

GB 3838-2002

铜、锌、硒、砷、汞、镉、铬、铁、锰

电镀污染物排放标准

GB 21900-2008

铬、镍、镉、银、铅、汞、铜、锌、铁、铝

◆其他与金属含量检测相关的标准

标准

标准号

涉及到的金属离子

淀粉及其制品重金属含量第1部分:原子吸收光谱法测定砷含量

GB/T20380.1-2006

淀粉及其制品重金属含量第3部分:电热原子吸收光谱法测定铅含量

GB/T20380.3-2006

淀粉及其制品重金属含量第4部分:电热原子吸收光谱法测定镉含量

GB/T20380.4-2006

重金属精矿产品中有害元素的限量规范

GB20424-2006

铅、砷、铬、汞、镉

纺织品重金属的测定第1部分:原子吸收分光光度法

GB/T17593.1-2006

镉、钴、铬、铜、镍、铅、锑、锌

染料产品中10种重金属元素的限量及测定

GB20814-2006

镉、钴、铬、铜、镍、铅、锑、锌

皮革和毛皮化学试验重金属含量的测定

GB/T22930-2008

铅、镉、镍、铬、钴、铜、锑、砷、汞

医用输液、输血、注射器具检验方法 第1部分:化学分析方法

GBT 14233.1-2008

锌、铅、铬、铜

生态纺织品技术要求

GB/T 18889-2009

锑、砷、钴、铅、镉、铬(六价)、铜、镍、汞

橡胶中铜含量的测定原子吸收光谱法 

GB/T 7043.1-2001

橡胶制品 镉含量的测定 原子吸收光谱法

GB/T 29607-2013

润滑油及添加剂中钼含量的测定 原子吸收光谱法

SH/T 0605-2006

表面处理溶液 金属元素含量的测定 电感耦合等离子体原子发射光谱法

GB/T 24916-2010

铝、钠、钙、镁、铁、铜、铬、铅、锌、锰、镍、锑

食品安全国家标准:食品中污染物限量

GB 2762-2012

铅、镉、汞、砷、锡、镍、铬

室内装饰装修材料内涂料中有害物质限量

GB 18582-2008

铅、铬、镉、汞

 


针对于金属含量的检测,金畔能提供原子吸收光谱分析用的金属离子标准液:

原子吸光分析用金属离子标准液

  原子吸光分析(AAS)用于金属元素的定量分析,由于高灵敏度、高选择性、操作简单的优点,是分析水、土壤、生物样品重金属的重要分析方法。Wako提供的原子吸光用金属标准液获得JCSS(日本校准服务体系)证书,具有可靠性高和精确度高的特点。

产品编号

产品名称

浓度mg/L

包装

016-15471

Aluminum Standard Solution

铝Al

1000

100mL

016-18271

Aluminum Standard Solution

铝Al

100

100mL

010-15491

Antimony Standard Solution

锑Sb

1000

100mL

013-18281

Antimony Standard Solution

锑Sb

100

100mL

027-15321

Barium Standard Solution

钡Ba

1000

100mL

020-07481

Beryllium Standard Solution

铍Be

1000

100mL

021-12661

Bisumth Standard Solution

铋Bi

1000

100mL

023-14201

Bisumth Standard Solution

铋Bi

100

100mL

025-16581

Boron Standard Solution

硼B

1000

100mL

036-16171

Cadmium Standard Solution

镉Cd

1000

100mL

030-16211 

Cadmium Standard Solution

镉Cd

100

100mL

039-16161

Calcium Standard Solution

钙Ca

1000

100mL

036-17891

Calcium Standard Solution

钙Ca

100

100mL

039-11661

Cerium Standard Solution

铈Ce

1000

100mL

035-25311

Cesium Standard Solution

铯Cs

1000

100mL

030-16191

Chromium Standard Solution

铬Cr

1000

100mL

037-16221

Chromium Standard Solution

铬Cr

100

100mL

033-16181

Cobalt Standard Solution

钴Co

1000

100mL

039-17901

Cobalt Standard Solution

钴Co

100

100mL

033-16201

Copper Standard Solution

铜Cu

1000

100mL

034-16231

Copper Standard Solution

铜Cu

100

100mL

045-20111 

Dysprosium Stand Solution

镝Dy

1000

100mL

054-04361

Erbium Standard Solution

铒Er

1000

100mL

051-04251

Europium Standard Solution

铕Eu

1000

100mL

070-02481

Galdolinium Standard Solution

钆Gd

1000

100mL

070-05781

Gallium Standard Solution

镓Ga

1000

100mL

077-02131

Germanium Standard Solution

锗Ge

1000

100mL

077-01771

Gold Standard Solution

金Au

1000

100mL

081-04651

Holmium Standard Soltuion

钬Ho

1000

100mL

092-05841

Indium Standard Solution

铟In

1000

100mL

094-03841

Iron Standard Solution

铁Fe

100

100mL

091-03851

Iron Standard Solution

铁Fe

1000

100mL

127-02841

Lanthanum Standard Solution

镧La

1000

100mL

124-04291

Lead Standard Solution

铅Pb

1000

100mL

127-04301

Lead Standard Solution

铅Pb

100

100mL

129-05221

Lithium Standard Solution

锂Li

1000

100mL

122-02911

Lutetium Standard Solution

镥Lu

1000

100mL

136-12121

Magnesium Standard Solution

镁Mg

1000

100mL

136-13601

Magnesium Standard Solution

镁Mg

100

100mL

133-12131

Manganese Standard Solution

锰Mn

1000

100mL

139-12111

Manganese Standard Solution

锰Mn

100

100mL

130-14961

Molybdenum Standard Solution

钼Mo

1000

100mL

149-04841

Neodymium Standard Solution

钕Nd

1000

100mL

147-06461

Nickel Standard Solution

镍Ni

1000

100mL

144-06471

Nickel Standard Solution

镍Ni

100

100mL

146-04971

Niobium Standard Solution

铌Nb

1000

100mL

166-08111 

Palladium Standard Solution

钯Pd

1000

100mL

163-08121

Platinum Standard Solution

铂Pt

1000

100mL

165-17471

Potassium Standard Solution

钾K

1000

100mL

162-19941

Potassium Standard Solution

钾K

100

100mL

161-12831

Preseodymium Standard Solution

镨Pr

1000

100mL

183-00661

Rhodium Standard Solution

铑Rh

1000

100mL

188-01951

Rubidium Standard Solution

铷Rb

1000

100mL

195-08721

Samarium Standard Solution

钐Sm

1000

100mL

196-08751

Scandium Standard Solution

钪Sc

1000

100mL

192-13861

Selenium Standard Solution

硒Se

1000

100mL

192-06031

Silicon Standard Solution

硅Si

1000

100mL

199-06041

Silver Standard Solution

银Ag

1000

100mL

199-10831

Sodium Standard Solution

钠Na

1000

100mL

191-12111 

Sodium Standard Solution

钠Na

100

100mL

199-13871

Strontium Standard Solution

锶Sr

1000

100mL

206-08141

Tantalum Standard Solution

钽Ta

1000

100mL

209-17921

Tellurium Standard Solution

碲Te

1000

100mL

209-08871

Terbium Standard Solution

铽Tb

1000

100mL

207-09151

Thulium Standard Solution

铥Tm

1000

100mL

202-16311

Tin Standard Solution

锡Sn

1000

100mL

209-06171

Titanium Standard Solution

钛Ti

1000

100mL

203-08151

Tungstem Standard Solution

钨W

1000

100mL

221-01851

Vanadium Standard Solution

钒V

1000

100mL

257-00131

Ytterbium Standard Solution

镱Yb

1000

100mL

250-00121

Yttrium Standard Solution

钇Y

1000

100mL

264-01421

Zinc Standard Solution

锌Zn

1000

100mL

261-01431

Zinc Standard Solution

锌Zn

100

100mL

263-00891

Zirconium Standard Solution

锆Zr

1000

100mL

原子吸光分析用金属离子标准液

JCSS级别离子标准液