[1]　

Yamamoto, Y., Gotoh, S., Korogi, Y., Seki, M., Konishi, S., Ikeo, S., Sone, N., Nagasaki, T., Matsumoto, H., Muro, S., Ito, I., Hirai, T., Kohno, T., Suzuki, Y. and Mishima, M.: Nat. Methods,14, 1097 (2017).

Hawkins, F., Kramer, P., Jacob, A., Driver, I., Thomas, DC., McCauley, KB., Skvir, N., Crane, AM., Kurmann, AA., Hollenberg, AN., Nguyen, S., Wong, BG., Khalil, AS., Huang, SX., Guttentag, S., Rock, JR., Shannon, JM., Davis, BR. and Kotton, DN.: J. Clin. Invest., 127, 2277 (2017).

Konishi, S., Gotoh, S., Tateishi, K., Yamamoto, Y., Korogi, Y., Nagasaki, T., Matsumoto, H., Muro, S., Hirai, T., Ito, I., Tsukita, S. and Mishima, M.: Stem Cell Reports, 6, 18 (2016).

Kido, T., Koui, Y., Suzuki, K., Kobayashi, A., Miura, Y., Chem, EY., Tanaka, M. and Miyajima, A.: Stem Cell Reports, 5, 508 (2015).

Gotoh, S., Ito, I., Nagasaki, T., Yamamoto, Y., Konishi, S., Korogi, Y., Matsumoto, H., Muro, S., Hirai, T., Funato, M., Mae, S., Toyoda, T., Sato-Otsubo, A., Ogawa, S., Osafune, K. and Mishima, M.: Stem Cell Reports, 3, 394 (2014).

BAMBANKER^® 是一种无血清细胞冻存液。可在-80℃长期保存细胞（肿瘤细胞和常规细胞）。

P3U1

（小鼠骨髓瘤细胞系）

K562

（人白血病细胞系）

human γδT cells

（人γδT细胞）

 Daudi

（人 B细胞系）

PC12

（大鼠源肾上腺嗜铬细胞瘤）

human B cell line 

（人B细胞系）

OKT4

（小鼠杂交瘤细胞）

B细胞系

(猴）

3T3‐L1（小鼠前脂肪细胞系）

A431（人扁平上皮癌细胞系）

BAEC（牛主动脉血管内皮细胞系）

Balb/3T3（小鼠成纤维细胞系）

C2C12（小鼠骨骼肌细胞系）

 Daudi（人B细胞系）

ECV304（人脐静脉内皮细胞系）

H295R（肾上腺皮质细胞）

HEK293（人胚胎肾细胞系）

HEK293T（人胚胎肾细胞系）

HeLa（人子宫颈癌细胞系）

HeLa S3（人子宫颈癌细胞系）

HepG2（人肝癌细胞系）

HFF（人正常成纤维细胞系）

Huh7（人肝癌细胞系）

Jurkat（人白血病T细胞系）

K562（人慢性骨髓性白血病细胞系）

KATOIII （人胃癌上皮细胞系）

KLM‐1（人胰腺癌细胞系）

MDCK（犬肾小管上皮细胞系）

MEF（小鼠胚胎成纤维细胞）

NIH3T3（小鼠胚胎皮肤细胞）

OKT4（小鼠杂交瘤细胞）

OP9（小鼠骨髓基质细胞）

P3U1（小鼠骨髓瘤细胞系）

PANC‐1（人胰腺癌细胞系）

PC12（大鼠源肾上腺嗜铬细胞瘤）

RPE（人视网膜上皮细胞系）

SNL（小鼠胚胎成纤维细胞）

TSU‐Pr1（人前列腺癌细胞系）

Vero（非洲绿猴肾细胞系）

human γδT cells （人γδT细胞）

human B cell line （人B细胞系）

HS-RMS-2

（多形性横纹肌肉瘤细胞系）

LNCaP clone FGC

（人前列腺癌细胞）

MEF-BL/6-1

（小鼠胚胎成纤维细胞）

猴B细胞系

人外周血活化淋巴细胞

永生化人肌肉细胞

小鼠脾脏活化淋巴细胞

小鼠ES细胞系

人胃上皮细胞

BAMBANKER® 无血清细胞

冻存液新版

Wako BAMBANKER冻存液（新手册）

细胞种类列举

BAMBANKER细胞冻存液

–冻存解冻步骤说明

（终端）.pdf

１.

Q：为什么我使用了BAMBANKER^® 来保存细胞，但是存活率依然不高？

A：请冻存前确保细胞处于生长对数期，并且冻存时细胞数目控制在5×10⁵~1×10⁷/mL冻存  液。

2.

Q：我们实验室已经有固定的冻存程序了，换了你们的BAMBANKER^® 可以还继续用原来程序降温的方法冻存吗？

A：虽然本产品可以无需程序降温冻存细胞，但如果通过程序降温盒等适当控制了温度下降的速度，效果更佳。

3.

Q：哪些细胞株（系）适合使用BAMBANKER^® 来进行细胞冷冻保存？

A：几乎所有细胞株（系）都可以使用BAMBANKER^® 进行冻存。对于较为宝贵的ES/iPS细胞的保存尤其适用。官网上所列举的细胞系均已经过测试验证。

但也并不排除可能有某些细胞株是不适合使用BAMBANER^® 来进行冻存的，用户在没有确认是否可使用时，建议在进行正式细胞冻存之前先进行预实验。

4.

Q：无血清冻存液相比传统含血清冻存液有什么优势？

A：无血清冻存液因不含有动物血清，质量更稳定，批间差小；同时未知生物成分或感染性物质污染细胞的几率也极低，尤其对于ES/iPS细胞等有可能用于再生医疗的细胞安全得以严格保障；可以直接冻存无血清培养的细胞，免去无血清再驯化的步骤；另外BAMBANKER^® 无血清细胞冻存液不需要像传统的血清冻存液需要程序降温，减少了用户的繁琐操作，节省了时间。

5.

Q：BAMBANKER^® 在冷冻保存细胞的过程中起什么作用？

A：BAMBANKER^® 无血清细胞冻存液使用了DMSO等作为保护剂，在冻存细胞时能以1℃/min左右的温度下降而逐渐冻结，在此过程中，细胞内的水分子被置换成冻结保护剂，抑制胞内和细胞周边的冰晶的形成，防止细胞膜和细胞器结构损伤，防止蛋白变质。

6.

Q：未使用的BAMBANKER^® 无血清细胞冻存液应该如何保存？

A：2-10℃避光保存。开封后尽快使用。请注意保质期为自生产日期起24个月。

7.

Q：BAMBANKER^® 能否使用于医疗领域?

A：BAMBANKER^® 仅供科研使用，不能使用于人体或医疗领域。

[1]

Zhang C., Seo J., Nakamura T.   (2018) Cellular Approaches in Investigating Argonaute2-Dependent RNA   Silencing. In: Okamura K., Nakanishi K. (eds) Argonaute Proteins. Methods in   Molecular Biology, vol 1680. Humana Press, New York, NY.

[2]

Sharma, A., M¨ucke, M., &   Seidman, C. E. (2018). Human induced pluripotent stem cell production and   expansion from blood using a non-integrating viral reprogramming vector.   Current Protocols in Molecular Biology,122, e58. doi: 10.1002/cpmb.58.

[3]

Souta Motoike, Mikihito Kajiya,   Nao Komatsu, et al. Cryopreserved clumps of mesenchymal stem   cell/extracellular matrix complexes retain osteogenic capacity and induce   bone regeneration. Stem Cell Res Ther. 2018; 9: 73. Published online 2018 Mar   21. doi: 10.1186/s13287-018-0826-0.

[4]

Konuma T1, Kohara C1, Watanabe   E2, et al. Monocyte subsets and their phenotypes during treatment with   BCR-ABL1 tyrosine kinase inhibitors for Philadelphia chromosome-positive   leukemia. Hematol Oncol. 2018 Apr;36(2):451-456. doi: 10.1002/hon.2497. Epub   2018 Feb 12.

[5]

Srijaya Thekkeparambil   Chandrabose, Sandhya Sriram, et al. Amenable epigenetic traits of dental pulp   stem cells underlie high capability of xeno-free episomal reprogramming.

Stem Cell Research & Therapy 2018 9:68.

[6]

Evans, Michael A. et al.   "Macrophage-Mediated Delivery of Light Activated Nitric Oxide Prodrugs with   Spatial, Temporal and Concentration Control." Chemical Science (2018): n.   pag. Web. doi:10.1039/C8SC00015H.

[7]

Jauregui, C.; Yoganarasimha, S.;   Madurantakam, P. Mesenchymal Stem Cells Derived from Healthy and Diseased   Human Gingiva Support Osteogenesis on Electrospun Polycaprolactone Scaffolds.   Bioengineering 2018, 5, 8.

[8]

Khamaikawin, Wannisa et al.   Modeling Anti-HIV-1 HSPC-Based Gene Therapy in Humanized Mice Previously   Infected with HIV-1. Molecular Therapy – Methods & Clinical Development ,   Volume 9,23-32.

[9]

Masako Okumura, Toyoaki Natsume,   Masato T Kanemaki, Tomomi Kiyomitsu. Optogenetic reconstitution reveals that   Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a   multi-arm ensemble. bioRxiv 277202; doi: https://doi.org/10.1101/277202

[10]

https://labchem.wako-chem.co.jp/journal/docs/proup10.pdf<链接>

[11]

Ince T A, Aster J C. In vitro   culture conditions for T-cell acute lymphoblastic leukemia/lymphoma: U.S.   Patent 9,683,217[P]. 2017-6-20.

[12]

Morris C D, Azadnia P, de Val N,   et al. Differential Antibody Responses to Conserved HIV-1 Neutralizing   Epitopes in the Context of Multivalent Scaffolds and Native-Like gp140   Trimers[J]. mBio, 2017, 8(1): e00036-17.<链接>

[13]

Lee K, Saetern O C, Nguyen A, et   al. Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain   Donors[J]. JoVE (Journal of Visualized Experiments), 2017 (119):   e55045-e55045.<链接>

[14]

Buenrostro J D, Corces R, Wu B,   et al. Single-cell epigenomics maps the continuous regulatory landscape of   human hematopoietic differentiation[J]. bioRxiv, 2017: 109843.<链接>

[15]

Edmonds R E, Garvican E R, Smith   R K W, et al. Influence of commonly used pharmaceutical agents on equine bone   marrow‐derived mesenchymal stem cell viability[J]. Equine veterinary journal,   2017, 49(3): 352-357.<链接>

[16]

Jitraruch S, Dhawan A, Hughes R   D, et al. Cryopreservation of Hepatocyte Microbeads for Clinical   Transplantation[J]. Cell transplantation, 2017.<链接>

[17]

Usarek E, Barańczyk-Kuźma A,   Kaźmierczak B, et al. Validation of qPCR reference genes in lymphocytes from   patients with amyotrophic lateral sclerosis[J]. PloS one, 2017, 12(3):   e0174317.<链接>

[18]

Gagnon E, Connolly A, Dobbins J,   et al. Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains During   Immunological Synapse Formation Using Donor-Quenching FRET and FLIM-FRET[J].   The Immune Synapse: Methods and Protocols, 2017: 259-289.<链接>

[19]

Foster K, Chaddock J, Penn C, et   al. Non-cytotoxic protein conjugates: U.S. Patent 9,474,807[P]. 2016-10-25.

[20]

Araki N, Iida M, Machida K.   Bioassay method for detecting physiologically active substance: U.S. Patent   9,316,588[P]. 2016-4-19.

[21]

Sazinsky S, Michaelson J S,   Sathyanarayanan S, et al. Antibodies to icos: U.S. Patent Application   15/076,867[P]. 2016-3-22

[22]

李凯. Studies on Innate Immune   Activation by HBV Infection and Its Sensing Mechanism in Hepatocytes[J].   2016.

[23]

Ip L R H. Effect of INPP4B loss   on DNA repair and treatment strategies in ovarian cancer[D]. UCL (University   College London), 2016.

[24]

Thakkar A. Novel hormonal   combination therapy for triple negative breast cancer[D]. University of   Miami, 2016.

[25]

Pakdaman Y. In-vitro   characterization of STUB1 mutations in recessively inherited spinocerebellar   ataxia-16[D]. The University of Bergen, 2016.

[26]

Caxaria S. Induced pluripotent   stem cells (iPSCs) for research and therapy: induction of hepatic   differentiation in iPSCs and evaluation of their quality as a model of in   vivo development in the context of coagulation[D]. UCL (University College   London), 2016.<链接>

[27]

Bayne R A, Donnachie D J,   Kinnell H L, et al. BMP signalling in human fetal ovary somatic cells is   modulated in a gene-specific fashion by GREM1 and GREM2[J]. MHR: Basic   science of reproductive medicine, 2016, 22(9): 622-633.<链接>

[28]

Friedrich D. HIF-1 [alpha]   Drives Fungal Immunity in Human Macrophages[D]. Universität zu Lübeck,   2016.<链接>

[29]

Yasuda M, Kawabata J,   Akieda-Asai S, et al. Guanylyl cyclase C and guanylin reduce fat droplet   accumulation in cattle mesenteric adipose tissue[J]. The Journal of   Veterinary Science, 2016.<链接>

[30]

Campa M J, Moody M A, Zhang R,   et al. Interrogation of individual intratumoral B lymphocytes from lung   cancer patients for molecular target discovery[J]. Cancer Immunology,   Immunotherapy, 2016, 65(2): 171-180.<链接>

[31]

Kobayashi T, Yagi Y, Nakamura T.   Development of Genome Engineering Tools from Plant-Specific PPR Proteins   Using Animal Cultured Cells[J]. Chromosome and Genomic Engineering in Plants:   Methods and Protocols, 2016: 147-155.<链接>

[32]

Shikata H, Kaku M, Kojima S I,   et al. The effect of magnetic field during freezing and thawing of rat bone   marrow-derived mesenchymal stem cells[J]. Cryobiology, 2016, 73(1):   15-19.<链接>

[33]

Hirakawa M, Matos T, Liu H, et   al. Low-dose IL-2 selectively activates subsets of CD4+ Tregs and NK   cells[J]. JCI insight, 2016, 1(18).<链接>

[34]

Durruthy-Durruthy J, Sebastiano   V, Wossidlo M, et al. The primate-specific noncoding RNA HPAT5 regulates   pluripotency during human preimplantation development and nuclear   reprogramming[J]. Nature genetics, 2016, 48(1): 44-52.<链接>

[35]

Caxaria S, Arthold S, Nathwani A   C, et al. Generation of integration-free patient specific iPS cells using   episomal plasmids under feeder free conditions[J]. Patient-Specific Induced   Pluripotent Stem Cell Models: Generation and Characterization, 2016: 355-366.<链接>

[36]

Nonomura Y, Otsuka A, Nakashima   C, et al. Peripheral blood Th9 cells are a possible pharmacodynamic biomarker   of nivolumab treatment efficacy in metastatic melanoma patients[J].   Oncoimmunology, 2016, 5(12): e1248327.<链接>

[37]

Burridge P W, Diecke S, Matsa E,   et al. Modeling cardiovascular diseases with patient-specific human   pluripotent stem cell-derived cardiomyocytes[J]. Patient-Specific Induced   Pluripotent Stem Cell Models: Generation and Characterization, 2016: 119-130.<链接>

[38]

Mendonça M C P, Soares E S, de   Jesus M B, et al. PEGylation of Reduced Graphene Oxide Induces Toxicity in   Cells of the Blood–Brain Barrier: An in Vitro and in Vivo Study[J]. Molecular   pharmaceutics, 2016, 13(11): 3913-3924.<链接>

[39]

Eldaim A, Hashimoto O, Ohtsuki   H, et al. Expression of uncoupling protein 1 in bovine muscle cells[J].   Journal of animal science, 2016, 94(12): 5097-5104.<链接>

[40]

Zhen A, Rezek V, Youn C, et al.   Stem-cell based engineered immunity against HIV infection in the humanized   mouse model[J]. JoVE (Journal of Visualized Experiments), 2016 (113):   e54048-e54048.<链接>

[41]

Bastian N A, Bayne R A,   Hummitzsch K, et al. Regulation of fibrillins and modulators of TGFβ in fetal   bovine and human ovaries[J]. Reproduction, 2016, 152(2): 127-137.<链接>

[42]

Eto K, Takayama N, Nakamura S,   et al. Method for producing differentiated cells: U.S. Patent 9,200,254[P].   2015-12-1.

[43]

Eto K, Takayama N, Nakamura S,   et al. Novel Method for Producing Differentiated Cells: U.S. Patent   Application 14/925,508[P]. 2015-10-28.

[44]

Yamashita J, Takeda M.   Cd82-positive cardiac progenitor cells: U.S. Patent Application   15/308,147[P]. 2015-4-16.

[45]

Cai Y, Sugimoto C, Arainga M, et   al. Preferential Destruction of Interstitial Macrophages over Alveolar   Macrophages as a Cause of Pulmonary Disease in Simian Immunodeficiency   Virus–Infected Rhesus Macaques[J]. The Journal of Immunology, 2015, 195(10):   4884-4891.<链接>

[46]

Kojima S I, Kaku M, Kawata T, et   al. Cranial suture-like gap and bone regeneration after transplantation of   cryopreserved MSCs by use of a programmed freezer with magnetic field in   rats[J]. Cryobiology, 2015, 70(3): 262-268.<链接>

[47]

Egawa E Y, Kitamura N, Nakai R,   et al. A DNA hybridization system for labeling of neural stem cells with SPIO   nanoparticles for MRI monitoring post-transplantation[J]. Biomaterials, 2015,   54: 158-167.<链接>

[48]

Durruthy J D, Sebastiano V.   Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs[J].   Stem Cells and Good Manufacturing Practices: Methods, Protocols, and   Regulations, 2015: 31-42.<链接>

[49]

Sato Y, Sasaki T, Takahashi S,   et al. Development of a highly reproducible system to evaluate inhibition of   cytochrome P450 3A4 activity by natural medicines[J]. Journal of Pharmacy   & Pharmaceutical Sciences, 2015, 18(4): 316-327.<链接>

[50]

Burridge P W, Holmström A, Wu J   C. Chemically defined culture and cardiomyocyte differentiation of human   pluripotent stem cells[J]. Current protocols in human genetics, 2015: 21.3.   1-21.3. 15.<链接>

[51]

Käding N. Hypoxia Regulates Host   Cell Metabolism and Thereby Enhancing Clamydia Pneumonia Growth[D]. Zentrale   Hochschulbibliothek Lübeck, 2015.<链接>

[52]

Lu S. Calcium Dependent   Regulatory Mechanism in Wolfram Syndrome: A Dissertation[J]. 2015.

[53]

Garvican E R, Cree S, Bull L, et   al. Viability of equine mesenchymal stem cells during transport and   implantation[J]. Stem cell research & therapy, 2014, 5(4): 1.<链接>

[54]

Deng X, Terunuma H, Nieda M.   Method for producing nk cell-enriched blood preparation: U.S. Patent   Application 14/508,745[P]. 2014-10-7.

[55]

Foster K, Chaddock J, Penn C, et   al. Non-cytotoxic protein conjugates: U.S. Patent 8,778,634[P]. 2014-7-15.

[56]

Ramathal C Y, Dumuthy-Durruthy   J, Pera R A R, et al. Generation of male germ cells: U.S. Patent Application   14/904,396[P]. 2014-7-10

[57]

Ince T A. Assays, methods and   kits for analyzing sensitivity and resistance to anti-cancer drugs,   predicting a cancer patient's prognosis, and personalized treatment   strategies: U.S. Patent Application 14/894,595[P]. 2014-6-4.

[58]

Nishio M, Saeki K.   Differentiation of human pluripotent stem cells into highly functional   classical brown adipocytes[J]. Methods Enzymol, 2014, 537: 177-197.<链接>

[59]

Durruthy-Durruthy J, Briggs S F,   Awe J, et al. Rapid and efficient conversion of integration-free human   induced pluripotent stem cells to GMP-grade culture conditions[J]. PloS one,   2014, 9(4): e94231.<链接>

[60]

Koido S, Homma S, Okamoto M, et   al. Treatment with Chemotherapy and Dendritic Cells Pulsed with Multiple   Wilms' Tumor 1 (WT1)–Specific MHC Class I/II–Restricted Epitopes for   Pancreatic Cancer[J]. Clinical Cancer Research, 2014, 20(16):   4228-4239.<链接>

[61]

Patz Jr E F. Antibodies   Expressed by Intratumoral B Cells as the Basis for a Diagnostic Test for Lung   Cancer[R]. DUKE UNIV DURHAM NC, 2014.<链接>

[62]

Kaku M, Shimasue H, Ohtani J, et   al. A case of tooth autotransplantation after long-term cryopreservation   using a programmed freezer with a magnetic field[J]. The Angle Orthodontist,   2014, 85(3): 518-524.<链接>

[63]

Kaku M, Koseki H, Kojima S, et   al. Cranial bone regeneration after cranioplasty using cryopreserved   autogenous bone by a programmed freezer with a magnetic field in rats[J].   CryoLetters, 2014, 35(6): 451-461.<链接>

[64]

Koido S, Kinoshita S, Mogami T,   et al. Immunological assessment of cryotherapy in breast cancer patients[J].   Anticancer research, 2014, 34(9): 4869-4876.<链接>

[65]

Sazuka S, Katsuno T, Nakagawa T,   et al. Fibrocytes are involved in inflammation as well as fibrosis in the   pathogenesis of Crohn's disease[J]. Digestive diseases and sciences, 2014,   59(4): 760-768.<链接>

[66]

Lin S L, Lee S Y, Lin Y C, et   al. Evaluation of mechanical and histological properties of cryopreserved   human premolars under short-term preservation: A preliminary study[J].   Journal of Dental Sciences, 2014, 9(3): 244-248.<链接>

[67]

Poole E, Reeves M, Sinclair J H.   The use of primary human cells (fibroblasts, monocytes, and others) to assess   human cytomegalovirus function[J]. Human Cytomegaloviruses: Methods and   Protocols, 2014: 81-98.<链接>

[68]

Garvican E R, Dudhia J, Alves A   L, et al. Mesenchymal stem cells modulate release of matrix proteins from   tendon surfaces in vitro: a potential beneficial therapeutic effect[J].   Regenerative medicine, 2014, 9(3): 295-308.<链接>

[69]

Skinner J A, Zurawski S M,   Sugimoto C, et al. Immunologic characterization of a rhesus macaque H1N1   challenge model for candidate influenza vaccine assessment[J]. Clinical and   Vaccine Immunology, 2014: CVI. 00547-14.<链接>

[70]

Terunuma H, Deng X, Nieda M.   Method for producing nk cell-enriched blood preparation: U.S. Patent   Application 14/780,394[P]. 2013-3-27.

[71]

Cho M, Yamazaki T, Endo M, et   al. Anti-Phospholipase D4 Antibody: U.S. Patent Application 14/375,266[P].   2013-1-31.

[72]

Bhandari S. Radiological,   clinical and laboratory based studies in the pathogenesis of desmoid tumours   in familial adenomatous polyposis[J]. 2013.

[73]

Gonzàlez Juncà A. Study of   molecular mechanisms implicated in the TGF-beta oncogenic effect in   Glioma[J]. 2013.

[74]

Koseki H, Kaku M, Kawata T, et   al. Cryopreservation of osteoblasts by use of a programmed freezer with a   magnetic field[J]. CryoLetters, 2013, 34(1): 10-19.<链接>

[75]

Naito H, Yoshimura M, Mizuno T,   et al. The advantages of three‐dimensional culture in a collagen hydrogel for   stem cell differentiation[J]. Journal of Biomedical Materials Research Part   A, 2013, 101(10): 2838-2845.<链接>

[76]

Stec M, Baran J, Szatanek R, et   al. Properties of monocytes generated from haematopoietic CD34+ stem cells   from bone marrow of colon cancer patients[J]. Cancer Immunology,   Immunotherapy, 2013, 62(4): 705-713.<链接>

[77]

Müller L, Brighton L E, Carson J   L, et al. Culturing of human nasal epithelial cells at the air liquid   interface[J]. Journal of visualized experiments: JoVE, 2013 (80).<链接>

[78]

Kalaszczynska I, Ruminski S,   Platek A E, et al. Substantial differences between human and ovine   mesenchymal stem cells in response to osteogenic media: how to explain and   how to manage?[J]. BioResearch open access, 2013, 2(5): 356-363.<链接>

[79]

Tamai Y, Hasegawa A, Takamori A,   et al. Potential Contribution of a Novel Tax Epitope–Specific CD4+ T Cells to   Graft-versus-Tax Effect in Adult T Cell Leukemia Patients after Allogeneic   Hematopoietic Stem Cell Transplantation[J]. The Journal of Immunology, 2013,   190(8): 4382-4392.<链接>

[80]

Kasai K, Nakashima H, Liu F, et   al. Toxicology and biodistribution studies for MGH2. 1, an oncolytic virus   that expresses two prodrug-activating genes, in combination with prodrugs[J].   Molecular Therapy-Nucleic Acids, 2013, 2: e113.<链接>

[81]

Deng X, Terunuma H, Nieda M.   Method for producing nk cell-enriched blood preparation: U.S. Patent   Application 13/980,777[P]. 2012-1-17.

[82]

Somm E, Bonnet N, Martinez A, et   al. A botulinum toxin–derived targeted secretion inhibitor downregulates the   GH/IGF1 axis[J]. The Journal of clinical investigation, 2012, 122(9):   3295.<链接>

[83]

Takaoka E, Sonobe H, Akimaru K,   et al. Multiple sites of highly amplified DNA sequences detected by molecular   cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell   line[J]. American journal of cancer research, 2012, 2(2): 141.<链接>

[84]

Fahlbusch F B, Dawood Y, Hartner   A, et al. Cullin 7 and Fbxw 8 expression in trophoblastic cells is regulated   via oxygen tension: implications for intrauterine growth restriction?[J]. The   Journal of Maternal-Fetal & Neonatal Medicine, 2012, 25(11): 2209-2215.<链接>

[85]

Aloé S, Weber F, Behr B, et al.   Modulatory effects of bovine seminal plasma on uterine inflammatory   processes[J]. Reproduction in domestic animals, 2012, 47(1): 12-19.<链接>

[86]

Gupta A, Bhakta S. An integrated   surrogate model for screening of drugs against Mycobacterium tuberculosis[J].   Journal of antimicrobial chemotherapy, 2012, 67(6): 1380-1391.<链接>

[87]

Saeki K. Feeder-Free Culture for   High Efficiency Production of Subculturable Vascular Endothelial Cells from   Human Embryonic Stem Cells[J]. Human Embryonic and Induced Pluripotent Stem   Cells: Lineage-Specific Differentiation Protocols, 2012: 277-294.<链接>

[88]

Yamazaki T, Okabe H, Kobayashi   S, et al. Cancer stem cell mass and process for production thereof: U.S.   Patent Application 13/878,181[P]. 2011-10-6.

[89]

Deng X, Terunuma H, Nieda M.   Method for producing nk cell-enriched blood product: U.S. Patent Application   13/577,476[P]. 2011-2-4.

[90]

Sugii S, Kida Y, Berggren W T,   et al. Feeder-independent ips cell derivation from human and mouse adipose   stem cells[J]. Nature protocols, 2011, 6(3): 346.<链接>

[91]

Shinada T, Akimoto T, Zhu Y, et   al. Modulation of viability of live cells by focused ion‐beam exposure[J].   Biotechnology and bioengineering, 2011, 108(1): 222-225.<链接>

[92]

Huang M S, Chang W J, Huang H M,   et al. Effects of transportation time after extraction on the magnetic   cryopreservation of pulp cells of rat dental pulp[J]. Journal of Dental   Sciences, 2011, 6(1): 48-52.<链接>

[93]

Sato D, Suzuki Y, Kano T, et al.   Tonsillar TLR9 expression and efficacy of tonsillectomy with steroid pulse   therapy in IgA nephropathy patients[J]. Nephrology Dialysis Transplantation,   2011, 27(3): 1090-1097.<链接>

[94]

Kamada H, Kaku M, Kawata T, et   al. In-vitro and in-vivo study of periodontal ligament cryopreserved with a   magnetic field[J]. American Journal of Orthodontics and Dentofacial   Orthopedics, 2011, 140(6): 799-805.<链接>

[95]

Bui H T, Wakayama S, Mizutani E,   et al. Essential role of paternal chromatin in the regulation of   transcriptional activity during mouse preimplantation development[J].   Reproduction, 2011, 141(1): 67-77.<链接>

[96]

Takata Y, Kishine H, Sone T, et   al. Generation of iPS cells using a BacMam multigene expression system[J].   Cell structure and function, 2011, 36(2): 209-222.<链接>

[97]

Benko Z, Zhao R Y. Zeocin for   selection of bleMX6 resistance in fission yeast[J]. Biotechniques, 2011,   51(1): 57-60.<链接>

[98]

Abedini S, Kaku M, Kawata T, et   al. Effects of cryopreservation with a newly-developed magnetic field   programmed freezer on periodontal ligament cells and pulp tissues[J].   Cryobiology, 2011, 62(3): 181-187.<链接>

[99]

Oshima-Sudo N, Li Q, Hoshino Y,   et al. Optimized method for culturing outgrowth endothelial progenitor   cells[J]. Inflammation and Regeneration, 2011, 31(2): 219-227.<链接>

[100]

Araki N. Bioassay method for   antibody against thyroid-stimulating hormone receptor, measurement kit for   the antibody, and novel genetically modified cell for use in the bioassay   method or the measurement kit: U.S. Patent Application 13/381,402[P]. 2010-6-24.

[101]

Foster K, Chaddock J, Marks P,   et al. Fusion proteins: U.S. Patent 7,659,092[P]. 2010-2-9.

[102]

Mieno S, Boodhwani M, Robich M   P, et al. Effects of diabetes mellitus on VEGF‐induced proliferation response   in bone marrow derived endothelial progenitor cells[J]. Journal of cardiac   surgery, 2010, 25(5): 618-625.<链接>

[103]

Kaku M, Kamada H, Kawata T, et   al. Cryopreservation of periodontal ligament cells with magnetic field for   tooth banking[J]. Cryobiology, 2010, 61(1): 73-78.<链接>

[104]

Kawata T, Kaku M, Fujita T, et   al. Water molecule movement by a magnetic field in freezing for tooth   banking[J]. Biomedical Research, 2010, 21(4).<链接>

[105]

Lee S Y, Chiang P C, Tsai Y H,   et al. Effects of cryopreservation of intact teeth on the isolated dental   pulp stem cells[J]. Journal of Endodontics, 2010, 36(8): 1336-1340.<链接>

[106]

Huang Y H, Yang J C, Wang C W,   et al. Dental stem cells and tooth banking for regenerative medicine[J].   Journal of Experimental & Clinical Medicine, 2010, 2(3):   111-117.<链接>

[107]

Kwon H J, Enomoto T, Shimogawara   M, et al. Benchmarks[J]. Biotechniques, 2010, 48: 460-462.<链接>

[108]

Shimizu Y, Takamori A,   Utsunomiya A, et al. Impaired Tax‐specific T‐cell responses with insufficient   control of HTLV‐1 in a subgroup of individuals at asymptomatic and smoldering   stages[J]. Cancer science, 2009, 100(3): 481-489.<链接>

[109]

Park H S, Cho S G, Park M J, et   al. Bone marrow T cells are superior to splenic T cells to induce chimeric   conversion after non-myeloablative bone marrow transplantation[J]. The Korean   journal of internal medicine, 2009, 24(3): 252.<链接>

[110]

Enosawa S, Miyamoto Y, Ikeya T.   Frozen cell immobilized product, primary hepatocyte culture tool, and method   for producing primary hepatocyte culture tool: U.S. Patent Application   12/738,809[P]. 2008-9-11.

[111]

DePinho R A, Stommel J M.   Receptor tyrosine kinase profiling: U.S. Patent Application 12/450,820[P].   2008-4-11.

[112]

Mieno S, Clements R T, Boodhwani   M, et al. Characteristics and Function of Cryopreserved Bone Marrow–Derived   Endothelial Progenitor Cells[J]. The Annals of thoracic surgery, 2008, 85(4):   1361-1366.<链接>

[113]

Warren C. The Response of HN4   Cells to Porphyromonas gingivalis DNA[D]. , 2008.

[114]

Hikichi T, Wakayama S, Mizutani   E, et al. Differentiation potential of parthenogenetic embryonic stem cells   is improved by nuclear transfer[J]. Stem Cells, 2007, 25(1): 46-53.<链接>

[115]

Zaidi S K, Pande S, Pratap J, et   al. Runx2 deficiency and defective subnuclear targeting bypass senescence to   promote immortalization and tumorigenic potential[J]. Proceedings of the   National Academy of Sciences, 2007, 104(50): 19861-19866.<链接>

[116]

Hikichi T, Wakayama S, Mizutani   E, et al. Differentiation potential of parthenogenetic embryonic stem cells   is improved by nuclear transfer[J]. Stem Cells, 2007, 25(1): 46-53.<链接>

[117]

Liu D G, Kobayashi T, Onishi A,   et al. Relation between human decay‐accelerating factor (hDAF) expression in   pig cells and inhibition of human serum anti‐pig cytotoxicity: value of   highly expressed hDAF for xenotransplantation[J]. Xenotransplantation, 2007,   14(1): 67-73.<链接>

[118]

Ishii H, Iinuma A, Osumi K, et   al. Canine tumor treatment method, pharmaceutical formulation applied   thereto, and method of cryogenically preserving cells used therewith: U.S.   Patent Application 11/465,892[P]. 2006-8-21.

[119]

Hatoya S, Sugiyama Y, Torii R,   et al. Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC   of canine oocytes[J]. Theriogenology, 2006, 66(5): 1083-1090.<链接>

[120]

Sasaki M, Kato Y, Yamada H, et   al. Development of a novel serum‐free freezing medium for mammalian cells   using the silk protein sericin[J]. Biotechnology and applied biochemistry,   2005, 42(2): 183-188.<链接>

[121]

Haynes J E. Pseudonyms of   Authors: Including Anonyms and Initialisms[M]. JE Haynes, 1882.<链接>