特定芳香胺类分析用|环境分析纯试剂(其他)|环境分析纯试剂|试剂|关东化学株式会社

致癌等被指出特定胺(24种)形成偶氮色素,关于健康被害防止的观点出发,纤维制品等使用自我克制向对应的进行着。本公司,这些特定胺分析用混合标准液新发售的同时,本考试需要的试剂类问题广泛的产品阵容。以下是分析需要。混合标准液进行介绍。

特定芳香胺类分析用|环境分析纯试剂(其他)|环境分析纯试剂|试剂|关东化学株式会社

特定芳香胺类混合标准溶液(21种)的分析例

特定芳香胺类分析用|环境分析纯试剂(其他)|环境分析纯试剂|试剂|关东化学株式会社

产品名 包装 产品编号
特定芳香胺类混合标准溶液(21种Specific aromatic amines standard solution 2 mL×5 38209 – 96
1 . <em>o(^</em> -トルイジン [95 – 53 – 4 ] 2 . 2,4 -キシリジン [95 – – 1]68
3 . 2,6 -キシリジン 87 – 62 – 7][ 4 . <em>o(^</em> -アニシジン 90 : 0][
5 . p -クロロアニリン 106 – 47 – 8][ 6 . p -クレシジン [120 – – 8]71
7 . 2,4,5 -トリメチルアニリン [137 – 17]- 7 8 . 4 -クロロ- <em>o(^</em> -トルイジン [95 – 69 – 2 ]
9 . 2,4 -トルイレンジアミン 95 – 80 – 7][ 10 . 2,4 -ジアミノアニソール 615 – 05 – 4][
11 . 2 -アミノ- 4 -ニトロトルエン 99 : 55 – 8][ 12 . 4 -アミノアゾベンゼン 60 – 09 – 3][
13 . 4,4 -オキシジアニリン [101 – 80 – 4 ] 14 . 4,4 -ジアミノビフェニルメタン [101 – 77 – 9 ]
15 . <em>o(^</em> -アミノアゾトルエン 56 – 3][97 – 16 . 3,4,3 -ジメチル- 4 -ジアミノビフェニルメタン 88 – 0 -][838
17 . 3,3 -ジメチルベンジジン 119 – 93 – 7][ 18 . 4,4 -チオジアニリン [139 – 65 – 1 ]
19 . 4,4 -メチレン-ビス-( 2 -クロロアニリン) [101 – 14 – 4 ] 20 . 3,3 -ジクロロベンジジン [91 – 94 – 1 ]
21 . 3,3 -ジメトキシベンジジン [119 – 90 – 4 ]
(each 100毫克/ L in Acetonitrile)

※组成被授予的号码,上述色谱法高峰No .。

产品名 包装 产品编号
特定芳香胺类混合标准溶液(24种)Azodyes – Mix 1毫升 49862 – 71
10毫升 49863 – 18
4,4 -メチレンジアニリン [101 – 77 – 9 ] 2 -メトキシ- 5 -メチルアニリン [120 – – 8]71
4,4 -ジアミノジフェニルエーテル [101 – 80 – 4 ] 2 -ナフチルアミン [91 – – 8 ] 59
ベンジジン 92 – 87 – 5][ 2,4,5 -トリメチルアニリン [137 – 17]- 7
4,4 -ジアミノ- 3 -ジメチルジフェニルメタン为期3 88 – 0 -][838 4 -クロロ- 2 -メチルアニリン [95 – 69 – 2 ]
2,4 -トルエンジアミン 95 – 80 – 7][ 3,4,3 -ジクロロ- 4 -ジアミノジフェニルメタン [101 – 14 – 4 ]
2,4 -ジアミノアニソール 615 – 05 – 4][ 4 -アミノアゾベンゼン 60 – 09 – 3][
4,4 -ジアミノジフェニルスルフィド [139 – 65 – 1 ] 3,3 -ジクロロベンジジン [91 – 94 – 1 ]
3,3 -ジメチルベンジジン 119 – 93 – 7][ <em>o(^</em> -アミノアゾトルエン 56 – 3][97 –
3,3 -ジメトキシベンジジン [119 – 90 – 4 ] 5 -ニトロ- <em>o(^</em> -トルイジン 99 : 55 – 8][
<em>o(^</em> -トルイジン [95 – 53 – 4 ] 4 -アミノビフェニル [92 – 67 – 1 ]
<em>o(^</em> -アニシジン 90 : 0][ 2,4 -ジメチルアニリン [95 – – 1]68
p -クロロアニリン 106 – 47 – 8][ 2,6 -ジメチルアニリン 87 – 62 – 7][
(each 10毫克/ L in Acetonitrile)

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上海金畔生物科技有限公司提供 T细胞分离用尼龙毛柱

上海金畔生物科技有限公司提供 T细胞分离用尼龙毛柱

在研究体内及体外的免疫系统时,分离淋巴细胞群是一个关键步骤。因为现在市场上并没有针对B细胞的特异性抗血清,所以分离淋巴细胞中的T细胞并不是十分方便。T细胞尼龙毛分离法利用了尼龙毛对B细胞的亲和力,从而使T细胞在没有严重损伤的情况下达到的足够的纯度。因为这个方法不像流式和磁珠费用昂贵,而且操作简便,所需要的条件也不高,是一种非常实用的方法。

首先要指出的是实验中所使用的是分离T细胞专用的尼龙毛(Nylon Fiber),不是化工上的尼龙纤维,曾经有人问过我这种问题,如果你买错了,可是做不出来的!

现在市面上有尼龙毛填料,也已经有了商品化的尼龙毛柱子,这两者的区别主要是以下两点:1.商品化的柱子的填料量是已经定好的,有一个确定的上样量范围。而自己买填料装柱可以控制填料量,也就是可以控制上样量,这对于样本量非常少,或是样本量非常大的实验需求非常合适。2.商品化的柱子已经经过了无菌处理(一般是辐射灭菌),而自己装柱当然就要自己灭菌了。

操作方法:

1.秤取1.5g尼龙毛装入20-30ml玻璃容器或注射器内(要可以灭菌的),填料的体积控制在15毫升左右(注射器上有标记,所以还是比较好控制的),因为尼龙毛的松紧关系到T细胞的回收率,所以要特别注意。注射器下端配接5厘米左右长的带有弹簧夹的橡皮管。

2. 加入大量的PBS平衡柱子后,用铝箔包裹,并置于适当的容器内,高压灭菌15分钟。

(商品化尼龙毛柱以上步骤可以省略,经过PBS平衡后可以直接进行下列步骤。)

3. 灭菌的尼龙毛柱垂直固定后放于超净台内,顶部以铝箔覆盖,避免污染。

4. 注射器下端,橡皮管,弹簧夹用无水酒精消毒。打开弹簧夹调节流速在大约3-4ml/min。

5.首先,加入3-4倍柱体的无血培养基平衡,之后加入相同体积的含有血清的培养基平衡(上述培养基都要预热),最后等培养基液面接近柱填料液面时,关闭弹簧夹。

6. 样本细胞悬浮液浓度控制在约5×108cells/ml,上样量一般是1/3-1/5柱体积。样品加入之后,再加入少量培养液,然后关闭弹簧夹。

7.覆盖铝箔,垂直固定,置于消毒过的容器中,于培养箱内37℃孵育1小时。此过程中柱子必须要保持垂直状态。

8. 从培养箱中拿出柱子,置于超净台内,除去铝箔。接上按照4.中的方法消毒的弹簧夹和橡皮管,加入适当体积经37℃预热的培养基,打开弹簧夹控制流速在3-4 mL/min,流出约5ml之后,流出的培养液基变得不透明,此时其中含有T细胞,收集这一部分培养基。

9. 基本上何时结束收集取决于流出液体中细胞的浓度,实际上,收集的量达到一个柱体积时就可以停止收集,因为即使收集更多的量,回收率几乎不会增加。

10 .上述操作后,实验结果如下:

细胞的回收率为: 15~20 %

T细胞含量: 85~95 %

B细胞污染率: 1 5 %

多数细胞被确定为T细胞。

(注)收集粘附于尼龙毛上的细胞时,将T细胞收集完毕后的尼龙毛再无菌操作的状态下取出,转移到适当的容器中,用镊子小心的将尼龙毛松开,粘附的细胞可以洗到培养基中。或者将注射器芯插入注射器内,通过压力使的粘附的细胞随着液体流出。

更多产品,更多优惠,请联系我们!
上海金畔生物科技有限公司
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邮 编: 201208
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订货热线:15221999938
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com

多烯类抗真菌药成分


产品编号 产品名称 产品规格 产品等级 产品价格
019-13364 Amphotericin B 两性霉素B 100mg
015-13361 Amphotericin B 两性霉素B 250mg
011-13363 Amphotericin B 两性霉素B 1g

多烯类抗真菌药成分


两性霉素B(Amphotericin B)

CAS No. 1397-89-3

C47H73NO17=924.08

力価:750μg/mg 以上

可溶性溶剂:DMSO

用途(作用):多烯类抗菌剂。通过抑制细胞膜杀灭细菌。

多烯类抗真菌药成分

相关资料详情请查看:http:///pdf/show/80.html



TWEEN® 20 吐温20 AMRESCO 9005-64-5

名称:TWEEN® 20 吐温20

品牌:AMRESCO

订货号:9005-64-5

TWEEN&reg; 20 吐温20                                                        AMRESCO                                                        9005-64-5

咨询此产品

产品介绍

0777
TWEEN® 20

GradeREAGENT GRADE

CAS Number: 9005-64-5

Synonym(s): polysorbate 20, PEG 20 sorbitan monolaurate

Molecular Weight: N/A

Molecular Formula: N/A

Storage Condition: ROOM TEMPERATURE

Risk Statements: N/A

Safety Statements: N/A

 

Specifications

Arsenic (\%) < 0.0003

DNase (P/F) NONE

Heavy Metals (\%) < 0.001

Hydroxyl Number (mgKOH/g) 96 – 108

Identification (P/F) PASS

Moisture (KF) (\%) 3.0

Protease (P/F) NONE

Residue on Ignition (\%) 0.25

  • 最新回复


产品编号 产品名称 产品规格 产品等级 产品价格
043-16785 Distilled Water
 蒸馏水
500mL
047-16783 Distilled Water
 蒸馏水
2L
045-16784 Distilled Water
 蒸馏水
2L*6
041-16786 Distilled Water
 蒸馏水
5L
049-16787 Distilled Water
 蒸馏水
20L
316-90101 Distilled Water, Deionized, Sterile
 二次蒸馏水,去离子,无菌
100mL 日本基因
312-90103 Distilled Water, Deionized, Sterile
 二次蒸馏水,去离子,无菌
100mL*6 日本基因
318-90105 Distilled Water, Deionized, Sterile
 二次蒸馏水,去离子,无菌
500mL 日本基因
196-15645 Sterile Water, Endotoxin Free 500mL 细胞培养用

  我司为您准备了试验所需的一系列的水,请参考下表:

维生素C[L-抗坏血酸]检测试剂盒 L-Ascorbic Acid (L-Ascorbate) 货号:K-ASCO Megazyme中文站

维生素C[L-抗坏血酸]检测试剂盒

英文名:L-Ascorbic Acid (L-Ascorbate)

货号:K-ASCO

规格:40 assays (manual) / 400 assays

分析物意义:  蔬菜水果中的天然成分或加工食品中的添加成分

Megazyme检测试剂盒优点:反应快、试剂稳定 

For the specific assay of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products. Content:40 assays per kit

Colourimetric method for the determination of L-Ascorbic Acid 
in foodstuffs, feed, wine and other materials

Principle:
                        (5-methylphenazinium methosulphate)
(1) L-Ascorbic acid + R-H2 + MTT → dehydroascorbate +                                                                                         MTT-formazan + H+
                            (ascorbic acid oxidase)
(2) L-Ascorbic acid + ½O2 → dehydroascorbate + H2O

Kit size:                             40 assays (manual) / 400 (microplate)
                                          / 400 (auto-analyser)
Method:                             Spectrophotometric at 578 nm
Reaction time:                   ~ 8 min
Detection limit:                  0.175 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, jam, milk, dairy products
(e.g. cheese), dietetic foods, baby foods, processed meat, baking
additives, fruit and vegetables (e.g. tomato and potato),
pharmaceuticals, feed and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by MEBAK

Advantages

  • Very competitive price (cost per test)
     
  • All reagents stable for > 6 months after preparation
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats 

 

Q1. Is it possible to adapt the kit for use on various auto-analysers?

Yes.  If the assay format is not available for your auto-analyser you can supply the following parameters of your relevant auto-analyser to Megazyme and an appropriate assay format may be available:
Parameters:
Instrument make and model:
Light-path length:
Reagent addition volumes (e.g. R1 volume, R2 volume, R3 if applicable):
Suggested auto-analyser format for K-ASCO:
Prepare the reagents as described in the K-ASCO data booklet then prepare R1 and R2 as follows:
Preparation of R1: (90 assays)

Component

Volume

Bottle 1

5.6 mL

Bottle 4

0.225 mL

Distilled water

17.5 mL

Total volume

23.325 mL

Preparation of R2: (90 assays)

Component

Volume

Bottle 2

2.25 mL

Bottle 3

2.25 mL

Total volume

4.5 mL

EXAMPLE METHOD:
R1: 0.260 mL
Sample: ~ 0.005 mL
R2: 0.05 mL
Note: For accurate measurement of ascorbic acid each sample requires two measurements, one with AAO (bottle 4) present and one with AAO (bottle 4) absent.
In the auto-analyser format described above there is no option to read A1 as in the cuvette assay.  In this instance use an ascorbic acid calibration curve to calculate results.  Alternatively add 0.025 mL of bottle 2 and 3 separately to mimic the cuvette assay and allow the reading  A1 (this will require 3 reagent additions) OR it may be possible to add the MTT (bottle 2) with R1 as follows:
Preparation of R1: (90 assays) 

Component

Volume

Bottle 1

5.6 mL

Bottle 2

2.25 mL

Bottle 4

0.225 mL

Distilled water

17.5 mL

Total volume

25.575 mL

Preparation of R2: (90 assays)

Component

Volume

Bottle 2

2.25 mL

Bottle 3

2.25 mL

Total volume

4.5 mL

EXAMPLE METHOD:
R1: 0.285 mL
Sample: ~ 0.005 mL
R2: 0.025 mL 

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.

O形圈布流300℃式样φ56用G – 65 |柴田科技有限公司-环境检测设备、科学仪器的制造销售

产品详细

科学仪器

O形圈布流300℃式样φ56用G – 65

商品代码其他情报(式样)

这个产品比较表中追加
商品代码 054330 – 3602
型式
价格(不含税) 17,1000日元

上海金畔生物科技有限公司

527%2F%3Fc%3D34& ” 527%2F%3Fc%3D34&

Research Diets D12492, 60 kcal% 高脂饲料

Research Diets D12492, 60 kcal% 高脂饲料

Research Diets动物疾病造模饲料

Research Diets, Inc是为世界各地动物模型生物数据采集实验室提供饲料定制,纯化开源饮食和科学仪器的领先制造商。

上海金畔生物生物竭诚为广大科研工作者提供优质的动物造模饲料,并提供饲料定制服务,可根据您的要求修改饲料配方。

常备库存

DIO Series Diets/ AIN Diets/ Surwit Diets/ Western Diets

饲料定制服务

可根据您的要求快速定制您所需的配方,为您的实验助力

叶酸调节

依据所需调节配方中叶酸的含量

齐全的动物疾病造模饲料

肥胖、糖尿病/胰岛素抵抗、NAFLD(非酒精性脂肪肝)、代谢综合征、动脉粥样硬化/高胆固醇血症、高血压、癌症、基因表达、免疫学等。

 

DIO Series Diets (12.5KG/包)   订购/服务热线:15221999938
类别 货号 描述 应用
kcal fat D12492 (VHFD) Rodent Diet with 60% kcal% fat. 脂肪肝;炎症;肥胖;糖尿病
D12451 (HFD) Rodent Diet with 45% kcal% fat. 脂肪肝;炎症;肥胖;糖尿病
D12450B (LFD) Rodent Diet with 10% kcal% fat. 肥胖;糖尿病
Control Diet Options D12450H D12451 Match/ 17% Sucrose(蔗糖)
D12450J D12492 Match/ 7% Sucrose(蔗糖)
D12450K No Sucrose(无蔗糖)
Irradiated: kcal fat
γ照射
D12492i γ照射D12492,60% kcal% fat
D12451i γ照射D12451,45% kcal% fat
D12450Bi γ照射D12450B,10% kcal% fat
DIO Series Vitamin Mix 添加维生素 V10001 Vitamin Mix for AIN-76A Rodent Diet
Use at 10 gm/kg diet or 10 gm/ 4000 kcal digestible energy.
DIO Series Mineral Mix 添加矿物质 S10026 Mineral Mix for DIO Series Diets
Use at 10 gm/kg diet or 10 gm/ 4000 kcal digestible energy

 

AIN Diets (12.5KG/包) 
OpenSource Diets
/Cross Reference
AIN-76A-D10001 AIN-76A– widely used, balanced, purified rodent diet. 肥胖;糖尿病;骨质疏松症;高血压;动脉粥样硬化;代谢综合征
AIN-93G-D10012G AIN-93G– widely used, balanced, purified diet formulated for gestating and growing rodents.(怀孕与幼小的啮齿动物) 肥胖;糖尿病;骨质疏松症;高血压;动脉粥样硬化;代谢综合征
AIN-93M-D10012M AIN-93M– widely used, balanced, purified diet formulated for mature rodents.(成熟的啮齿动物)) 肥胖;糖尿病;骨质疏松症;高血压;动脉粥样硬化;代谢综合征
Irradiated γ照射 AIN-76A- D10001i γ照射AIN-76A- D10001
AIN-93G- D10012Gi γ照射AIN-93G- D10012G
AIN-93M- D10012Mi γ照射AIN-93M- D10012M
Vitamin Mix
添加维生素
AIN-76A- V10001 Vitamin Mix for AIN- 76A Rodent Diet
Use at 10 gm/kg diet or 10 gm/ 4000 kcal digestible energy.
AIN-93G- V10037 Vitamin Mix for Ain-93G Rodent Diet with Biotin
Use at 10 gm/kg diet or 10 gm/ 4000 kcal digestible energy.
AIN-93M- V10037 Vitamin Mix for Ain-93M Rodent Diet with Biotin
Use at 10 gm/kg diet or 10 gm/ 4000 kcal digestible energy.
Mineral Mix
添加矿物质
AIN-76A- S10001 Mineral Mix for AIN- 76A Rodent Diet
Use at 35 gm/kg diet or 35 gm/ 4000 kcal digestible energy.
AIN-93G- S10022G Mineral Mix for AIN-93G Growing Rodent Diet
Use at 35 g/kg diet or 35 g/4000 kcal digestible energy.
AIN-93M- S10022M Mineral Mix for AIN-93M Mature Rodent Diet
Use at 35 g/kg diet or 35 g/4000 kcal digestible energy.

 

Surwit Diets 使用氢化椰子油(> 99%饱和脂肪),为脂肪的主要来源 (12.5KG/包)  
订购/服务热线:15221999938
D12330 58 kcal% fat w/corn starch.Surwit Diet. 肥胖;糖尿病
D12328 Control: 11 kcal% fat w/corn starch.Surwit Diet 肥胖;糖尿病
D12331 58 kcal% fat w/sucrose.Surwit Diet 肥胖;糖尿病
D12329 Control: 11 kcal% fat w/sucrose.Surwit Diet 肥胖;糖尿病

 

Western Diets (12.5KG/包)  订购/服务热线:15221999938
OpenSource Diet Formula D12079B RD Western Diet 肥胖;糖尿病;骨质疏松症;高血压;动脉粥样硬化;代谢综合征
Irradiated
γ照射
D12079Bi γ照射D12079B

 

The “condensed milk” Diets (12.5KG/包)  订购/服务热线:15221999938
D12266B Purified match to C11024
Condensed Milk Diet
肥胖;糖尿病;高血压
D12489B Low fat condensed milk diet.Low-fat control to D12266B 肥胖;糖尿病;高血压

 

 Apo E or LDLR/Knockout mice Diets (12.5KG/包)  订购/服务热线:15221999938
D12108C 40 kcal% fat (mostly cocoa butter) and 1.25% cholesterol(胆固醇). 动脉粥样硬化 ;常与载脂蛋白E或低密度脂蛋白受体使用;基因敲除小鼠

 

专业提供配方定制服务,我们的定制客户来自世界各地,Research Diets, Inc拥有超过19,000种自主配方,能将您的产品快速运到您的手中。以下是我们给客户做的定制饲料的案例。

 

Custom Formula Purified Diets  Examples (12.5KG/包)   
D12327 D12451 Match/ 17% Sucrose 40 kcal% Fat (Hydrogenated Coconut Oil) With 40 kcal% Sucrose Diet
98121701 and 98052602 Low-Fat control diets for D12079B, without or with 0.15% Cholesterol
D12369B Ketogenic Diet
D12336 Purified diet matched to Paigen diet) for Atherosclerosis Research
A02082002B Methionine and Choline Deficient Diet) for NASH models
A06071301B (Methionine/choline deficient diet with 60 kcal% fat) and A06071302 (choline deficient diet with 0.1% Methionine) for NASH Models
D09100301 40 kcal% Fat (Mostly Vegetable Shortening), 20 kcal% Fructose, and 2% Cholesterol for NASH Models
D02022704 Low-fat diet with 60 kcal% Fructose and Starch Control (Matches the DIO diets)
D11707 and D11708B Low-fat diet with High Fructose and Starch Control (Matches the AIN-76A)
L10016A (Premix to Make Lieber DeCarli’s Diet (L10016) and Control (L10015)) for Alcoholism Studies
D05011703 (AIN-76A + 8% Sodium Chloride) for Hypertension studies

D-木糖检测试剂盒 D-Xylose assay Kit 货号:K-XYLOSE Megazyme中文站

D-木糖检测试剂盒

英文名:D-Xylose assay Kit

货号:K-XYLOSE

规格:100 assays (manual) / 1000 assays (microplate)

The D-Xylose test kit is a novel method for the specific, convenient and rapid measurement and analysis of D-xylose in plant extracts, culture media/supernatants and other materials.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of D-Xylose in fermentation
broths and hydrolysates of plant material and polysaccharides

Principle:
(xylose mutarotase)
(1) α-D-Xylose ↔ β-D-xylose

(β-xylose dehydrogenase)
(2) β-D-Xylose + NAD+ → D-xylonic acid + NADH + H+

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1300 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.7 mg/L
Application examples:
Analysis of D-xylose in fermentation broths and hydrolysates of plant
material and polysaccharides
Method recognition: Novel method

Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Rapid reaction (~ 6 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

 Q1. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. Can the K-XYLOSE test be used to measure D-xylose in urine samples?

Yes.  K-XYLOSE can be used to measure D-xylose in urine samples assuming that the D-xylose concentration is above the limit of detection for the kit assay after sample preparation.  An example sample preparation method for biological samples is provided in the K-XYLOSE data booklet. 

Q5. What level of glucose can the K-XYLOSE assay tolerate before interference is observed?

K-XYLOSE is able to specifically measure xylose in samples that contain up to 5 mg of glucose in the final assay reaction without any interference by the glucose.
D-木糖检测试剂盒 D-Xylose assay Kit 货号:K-XYLOSE  Megazyme中文站

Q6. Can oligosaccharides or polysaccharides be measured using the kit assay?

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

Q7. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

喷嘴手提瓶


产品编号 产品名称 产品规格 产品等级 产品价格
2138 红色喷嘴手提瓶 4L
2137  红色喷嘴手提瓶 2L
2136  白色喷嘴手提瓶 4L
2135 白色喷嘴手提瓶 2L

红色、白色喷嘴手提瓶

喷嘴手提瓶

优点


● 喷嘴也带有盖子,运输的时候更安全

● 喷嘴能够伸缩,并且能够固定形状



容量

白2L

白4L

红2L

红4L

尺寸

110×193×214H mm

110×193×290H mm

110×193×214H mm

110×193×290H mm

一箱

30个

24个

30个

24个

阿拉伯树胶七糖[纯度>95%][粉] Arabinoheptaose (powder) – 15mg 货号:O-AHP Megazyme中文站

阿拉伯树胶七糖[纯度>95%][粉]

英文名:Arabinoheptaose (powder) – 15mg

货号:O-AHP

规格:15 mg

CAS: 190852-27-8
Molecular Formula: C35H58O29
Molecular Weight: 924.8
Purity: > 95%

High purity Arabinoheptaose (powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Purity ~ 95%

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试剂蛋白肽测序仪配套试剂-日本和光wako-赖氨酰肽链内切酶


岛津蛋白测序仪配套试剂for SHIMADZU Protein Peptide Sequencer
#125-05061质谱分析前处理时最常用的蛋白质分解酶即赖氨酸肽链内切酶,该酶可以特异性切除赖氨酸基团C末端的多肽,可用于蛋白测序分析和Lys-X化合物的合成。若同时使用赖氨酸肽链内切酶和胰酶,可更好地切断赖氨酸基团的多肽,增加多肽的数量。

【详细说明】

岛津蛋白测序仪配套试剂for SHIMADZU Protein Peptide Sequencer

岛津蛋白测序仪配套试剂for SHIMADZU Protein Peptide Sequencer

品牌 产品编号 产品名称 中文名称 包装规格 用途
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 赖氨酰肽链内切酶,质谱等级 20ug x5 用于蛋白质研究
Wako 129-02541 Lysyl Endopeptidase® 赖氨酰肽链内切酶 1vial(10AU) 用于生物化学
Wako 207-10761 12% Trimethylamine Solution 12%三甲胺溶液 200ml 用于岛津蛋白肽测序
Wako 204-10771 Trifluoroacetic Acid 40ml 用于岛津蛋白肽测序
Wako 014-13831 37% Acetonitrile Solution 37%的乙腈溶液 200mL 用于岛津蛋白肽测序
Wako 021-09851 1-Chlorobutane 1-氯丁烷 200mL 用于岛津蛋白肽测序
Wako 054-04981 Ethyl Acetate 乙酸乙酯 200mL 用于岛津蛋白肽测序
Wako 161-14271 PTH-amino Acids Mobile Phase PTH-氨基酸流动相 1L 用于岛津蛋白肽测序
Wako 168-14161 5% Phenyl Isothiocyanate, n-Heptane Solution 5%异硫氰酸苯酯,正庚烷溶液 40mL 用于岛津蛋白肽测序
Wako 203-12201 25% Trifluoroacetic Acid Solution 25%溶液 40mL 用于岛津蛋白肽测序
Wako 239-63351 Wakopak Wakosil-PTH 4.6*250mm(S-PSQ) 色谱柱 1本 专用柱
Wako 293-57701 Negative Gel Stain MS Kit 负凝胶染色MS试剂盒 20回用 用于电泳
Wako 299-58901 Silver Stain MS Kit 银染剂MS试剂盒 20テスト 用于电泳
Wako 202-15951 Trypsin, from Porcine Pancreas, Mass Spectrometry Grade 胰蛋白酶,从猪胰脏中,质谱等级 20μg×5 用于蛋白质研究

注:

#125-05061质谱分析前处理时最常用的蛋白质分解酶即赖氨酸肽链内切酶,该酶可以特异性切除赖氨酸基团C末端的多肽,可用于蛋白测序分析和Lys-X化合物的合成。若同时使用赖氨酸肽链内切酶和胰酶,可更好地切断赖氨酸基团的多肽,增加多肽的数量。