总游离亚硫酸盐检测试剂盒 Total and Free Sulphite Assay Kit 货号:K-SULPH Megazyme中文站

总游离亚硫酸盐检测试剂盒

英文名:Total and Free Sulphite Assay Kit

货号:K-SULPH

规格:40 assays (manual) / 400 assays (microplate)

A rapid, simple, reliable and accurate method for the measurement and analysis of total sulfite (sulphite) and free sulfite in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for manual, auto-analyser and microplate formats.

Suitable for manual, auto-analyser and microplate formats.

Colourimetric methods for the determination of Total and Free
Sulfite in wine, fruit juice, foodstuffs and other materials

Principle:
The Total Sulfite assay is based on the reaction principle
between thiol groups and Ellman’s reagent


The Free Sulfite assay is based on the reaction principle of
SO2, fuchsin and aldehyde binding

Kit size: 40 assays (manual) / 400 (microplate)
/ 400 (auto-analyser)
Method: Total sulfite: Spectrophotometric at 405 nm
Free sulfite: Spectrophotometric at 575 nm
Total assay time: Total sulfite: ~ 6 min
Free sulfite: ~ 9 min
Detection limit: Total sulfite: ~ 5 mg/L
Free sulfite: ~ 2 mg/L
Application examples:
Wine, fruit juice, seafood, food stuffs and other materials
Method recognition:
Validated for red and white wines at the Bundesamt für Weinbau, Austria.
Used widely in the wine industry

Advantages

  • ”Ready to use" liquid stable Formulation
  • Very competitive price (cost per test)
  • All reagents stable for > 18 months
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

 Q1. What concentration of acetaldehyde in the sample causes interference in the total sulphite (TSO2) assay?

Interference by acetaldehyde is observed when the acetaldehyde concentration is higher than approximately 250 mg/L in the 0.05 mL sample using the TSO2 Manual Assay Procedure (see Table 1). 
At acetaldehyde concentrations higher than 250 mg/L the total sulphite reaction is slower than stated in the TSO2 Manual Assay Procedure but generates the expected absorbance change when the reaction is allowed to complete.  At a concentration of 1250 mg/L acetaldehyde in the sample, the TSO2 reaction takes ~ 15 minutes to complete (at 25°C).

[Acetadehyde]

(mg/L)

[SO2]

mg/L

 

A1 

 

A2 

 

ΔAtotal SO2 

 

% error

0 

300

0.042

1.477

1.435

0.0

31 

300

0.041

1.458

1.417

1.3

63 

300

0.042

1.502

1.460

-1.7

125

300

0.042

1.498

1.456

-1.4

250

300

0.041

1.466

1.425

 0.7

625

300

0.042

1.347

1.305

 9.1

1250

300

0.045

0.279

0.234

83.7

 

 

 

 












TABLE 1. 
Acetaldehyde Interference in the TSO2 Manual Assay.  Reactions were performed using the TSO2 Manual Assay Procedure in 1 cm path-length cuvettes at 25°C.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. How stable are the sulphite standards and samples?

When the samples are prepared and are stored at the appropriate pH (~ pH 8 for total sulphite and ~ pH 4 for free sulphite) they will be expected to lose ~ 2% sulphite per hour.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. Can K-SULPH be used to measure sulphite in food samples?

Yes.  K-SULPH can be used to measure total sulphite and free sulphite in food samples using the standard sample preparation procedures given below.

Sample preparation for Total Sulphite (TSO2)
Homogenise approx. 5 g of sample with 60 mL of distilled water using a mortar and pestle or standard homogeniser for approximately 2 min.  Adjust to approximately pH 8.0 using 1 M NaOH or 1 M HCl.  Quantitatively transfer the mixture to a 100 mL volumetric flask, fill up to mark with distilled water, mix and filter through Whatman No. 1 filter paper or centrifuge at 13000 x g.  If required, dilute the sample using 20 mm sodium phosphate buffer (pH 8.0).

Sample preparation for Free Sulphite (FSO2)
Homogenise approx. 5 g of sample with 60 mL of distilled water using a mortar and pestle or standard homogeniser for approx. 2 min.  Adjust to approximately pH 4.0 using 1 M NaOH or 1 M HCl.  Quantitatively transfer the mixture to a 100 mL volumetric flask, fill up to mark with distilled water, mix and filter through Whatman No. 1 filter paper or centrifuge at 13000 x g.  If required, dilute the sample using 1% (w/v) citric acid.

Notes:
1.It is highly recommended that samples are tested immediately after sample preparation.
2.The amount of sulphite obtained in the final sample must be within the detectable range of the test.  Since the amount of sulphite present will vary between samples the amount of original sample used in the preparation method may have to be experimentally determined.

Q6. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the 
K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch 
this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q7. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q8. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q9. I have some doubts about the appearance/quality of a kit component what should be done?

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seline; color: rgb(55, 55, 55); text-indent: 0px;”>微信号:jinpanbio
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seline; color: rgb(55, 55, 55); text-indent: 0px;”>Email:sales@jinpanbio.com

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抗α-突触核蛋白抗体[ mjfr1 ](ab138501)

种属反应性

与反应:人,Drosophila melanogaster
  • 应用 AB评论 说明 WB 1 / 10000。预测分子量:分子量为14 kDa。

    对于未使用1 / 1000 – 1 / 10000

    ihc-p 1 / 150。进行热介导抗原修复免疫组化染色协议开始之前。

    对于未使用1 / 15至1 / 300。

    IHC抗原检索协议(见http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol)。

    流式细胞 1 / 200。

    对于未使用1 / 20

    ab172730兔单克隆抗体,适用于该抗体的同型对照。 IP 1 / 600。

    对于未使用1 / 50

    ELISA 使用浓度为1~10µ克/毫升。 国际商会/如果 1 / 150。

    对于未使用1 / 15

  • 靶标

    • 功能可能参与了多巴胺的释放和转运的调控。诱导微管相关蛋白tau蛋白纤维化。减少各种凋亡刺激神经元的反应性,从而降低caspase-3的激活。
    • 组织特异性主要在脑中表达也在所有检查的组织,除肝低浓度表达。集中在突触前神经末梢。
    • 疾病相关导致异常聚合成纤维SNCA基因的改变与多种神经退行性疾病相关(synucleinopathies)。SNCA纤维聚集体代表阿尔茨海默病淀粉样斑块的主要非β淀粉样蛋白的成分,与路易体包裹体的主要成分。他们也在Lewy身上发现(LB)一样的突触夹杂物,胶质细胞包涵体和轴突球状与脑内铁沉积型1神经退行性疾病。帕金森病1帕金森病4Lewy体痴呆
    • 序列相似性属于α-突触核蛋白家族。
    • 结构域“非蛋白成分阿尔茨海默病淀粉样斑块的域(NAC)参与纤维的形成。中间的疏水区域形成长丝的核心。C端可调节聚合和确定的细丝直径。
    • 翻译后修饰磷酸化丝氨酸残基,主要。磷酸化的CK1似乎残留有别于其他残基磷酸化的激酶发生。磷酸化的ser-129在突触核蛋白病病变选择性和广泛的。在体外,磷酸化在ser-129促进不溶性纤维的形成。磷酸化的tyr-125 PTK2B依赖途径在渗透胁迫。神经突触核蛋白病的标志性病变包含α-突触核蛋白是由酪氨酸残基的硝化酪氨酸交联改性和可能产生的稳定的低聚物。泛素化。主要是diubiquitinated共轭形式。乙酰化在Met-1似乎正确折叠和本土的寡聚体结构是重要的。
    • 细胞定位Cytoplasm,胞浆。膜。核。细胞连接,突触。分泌。在多巴胺能神经元的界膜。
    • 以上信息来自:UniProt加入目标p37840UniProt协会通用蛋白质资源(UniProt)2010
      核酸研究38(2010):d142-d148

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    日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody

    日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody
    中文名称:抗丙二醛单克隆抗体
    英文名称:Anti Malondialdehyde(MDA) monoclonal antibody
    货号:MMD-030n
    规格:30ug
    Anti MDA monoclonal antibody  脂质过氧化生物标志物
    适用于免疫组化(仅供研究使用)
    About malondialdehyde (MDA)
    Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

    关于丙二醛(MDA)
    丙二醛(MDA)是衍生自脂质过氧化的主要醛之一。 MDA是高度反应性的醛,并与蛋白质中的赖氨酸残基反应。 与MDA和赖氨酸残基的反应导致众多数量的加合物的形成,例如二氢吡啶-赖氨酸(DHP-赖氨酸)型衍生物。 该单克隆抗体对MDA修饰的蛋白质,特别是DHP-赖氨酸型衍生物是特异性的。
    Specifications

    Clone #: 1F83
    Antigen: MDA-modified keyhole-lympet hemocyanine.
    Form: Frozen (100 碌g/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A.
    Application: Immunohistochemistry.
    Recommended antibody concentration is 0.5-1.0 碌g/mL on paraformaldehyde fixed tissue.
    Specificity: MSpecific for MDA-modified protein (especially DHP-lysine).
    Subclass: Mouse IgG2a(lambda)
    Storage: Less than -20掳C


    Specificity of anti MDA antibody.

    Immunohistochemical detection of MDA-modified protein in atherosclerotic aorta.
    Dr. N Shibata, Tokyo Women’s Medical University.
    技术参数
    克隆号#:1F83;
    抗原:MDA修饰的钥孔血蓝蛋白(KLH);
    性状:冻结(100μg/mL抗体在含有0.1%NaN3和0.5% BSA的10mM PBS中),经过蛋白A纯化;
    应用:免疫组织化学。对多聚甲醛固定的组织推荐抗体浓度为0.5-1.0μg/mL;
    特异性:特异性针对MDA修饰的蛋白(特别是DHP-赖氨酸)。
    亚基:小鼠IgG2a(lambda)
    储存:小于-20℃
    其他相关产品:
    ·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
    ·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
    ·  抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
    ·  己酰-赖氨酸加合物ELISA试剂盒 Hexanoyl-Lysine adduct(HEL) ELISA Kit
    References 参考文献

    1) Immunochemical detection of a lipofuscin-like fluorophore derivered from malondialdehyde and lysine.
    S Yamada, S Kumazawa, T Ishii, T Nakayama, K Itakura, N Shibata, M Kobayashi, K Sakai, T Osawa and K Uchida.
    J.Lipid Res. 42, p1187-1196 (2001)
    2) Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress.
    Muratori M, Tamburrino L, Marchiani S, Cambi M, Olivito B, Azzari C, Forti G, Baldi E
    Mol Med. 21,p109-122(2015). doi: 10.2119/molmed.2014.00158.

    日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody技术资料:http://www.jaica.com/e/technical_forum_nof.html#MDA
    日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody 说明书:
    http://www.jaica.com/e/pdf/mda_ab_manual.pdf

    氮、磷全全分析纯试剂|水质分析|环境分析纯试剂|试剂|关东化学株式会社

    横河电机㈱制的NP 600全氮磷·全自动测量装置专用的试剂,作为以下的销售的产品。另外,校正用的跨度液的各种标准液产品阵容。所以,氮和磷浓度各种调液成为可能。

    产品】【对象

    产品名 规格 产品编号
    NP 600试剂套(横河电机全氮磷·全自动测量装置用)NP 600 Reagents Set 氮、磷测量 28669 – 96
    ><套装内容

    A液 过氧二硫酸钾水溶液 一L
    B液 氢氧化钠溶液 250毫升
    C液 硫酸 250毫升
    D液 L – (+)-抗坏血酸水溶液 250毫升
    E液 钼酸铵混合溶液 250毫升
    F液 盐酸 250毫升
    容器 跨度液 250毫升

    * NP 600专用容器了,所以每更换容器试剂。
    *购买后尽量早点使用切,请避免长期保存。

    】【相关产品

    产品名 规格 包装 产品编号
    氮标准溶液(N : 1000毫克/ L)
    Nitrogen standard solution(N : 1000毫克/ L)
    水质试験用 100毫升 28670 – 23
    氮标准溶液(N : 10000毫克/ L)
    Nitrogen standard solution(N : 10000毫克/ L)
    水质试験用 100毫升 28671 – 23
    磷标准溶液(P : 10000毫克/ L)
    Phosphorus standard solution
    水质试験用 100毫升 33004 – 23
    蒸馏水
    Distilled water
    氮、磷测量 500毫升 11462 – 08
    20 L 11462 – 84

    试剂的咨询

    信息查询

    电话: 03 – 6214 – 1090

    传真: 03 – 3241 – 1047

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    PE附水龙头广口瓶


    产品编号 产品名称 产品规格 产品等级 产品价格
    2095 PE附水龙头广口瓶 5L
    2096 PE附水龙头广口瓶 10L

    PE附水龙头广口瓶

    PE附水龙头广口瓶

    容量

    5L

    10L

    螺丝尺寸

    20A

    20A

    规格

    常规

    常规

    一盒

    15个

    8个

                          *带内盖,水龙头是PP制

    AV旋塞


    产品编号 产品名称 产品规格 产品等级 产品价格
    3045 AV旋塞1型 见本页
    3046 AV旋塞2型 见本页

    AV旋塞

     

     

    AV旋塞

     

     


    原理

    规格

    简称

    连接样式

    适用尺寸

    1型

    软管/软管

    8-16mm

    2型

    凸头螺丝/软管

    螺丝口1/4  8-16mm

    ※Max.10Kgf/cm(0.98 MPa) 

     


    ◆优点・特色

     

    ●具有耐蚀性

    ●阀门由树脂制成

    ●轻便,可调节量度

     


    ◆案例・应用


     

    密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒 Raffinose/Sucrose/D-Glucose Assay Kit 货号:K-RAFGL Megazyme中文站

    密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒

    英文名:Raffinose/Sucrose/D-Glucose Assay Kit

    货号:K-RAFGL

    规格:120 assays of each per kit

    The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.

    Colourimetric method for the determination of Raffinose (also
    stachyose and verbascose), Sucrose and D-Glucose in
    legume seeds, plant materials, foodstuffs and feed

    Principle:
    (α-galactosidase)
    (1) Raffinose + stachyose + verbascose + H2O → D-galactose +
    sucrose


    (invertase)
    (2) Sucrose + H2O → D-glucose + D-fructose

    (glucose oxidase)
    (3) D-Glucose + H2O + O2 → D-gluconate + H2O2

    (peroxidase)
    (4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
    quinoneimine + 4H2O

    Kit size: 120 assays
    Method: Spectrophotometric at 510 nm
    Reaction time: ~ 20 min
    Detection limit: 100 mg/L
    Application examples:
    Analysis of grain legumes and other materials containing raffinose,
    stachyose and verbascose
    Method recognition: Used and accepted in food analysis

    Advantages

    • Very competitive price (cost per test)
    • All reagents stable for > 2 years after preparation
    • Simple format
    • Rapid reaction
    • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
    • Standard included

     

     Q1. Should the pH of the sample be adjusted even for samples in acidic media?

    The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
    Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
    Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

    Q2. There is an issue with the performance of the kit; the results are not as expected.

    If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

    1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
    2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
    3. State the kit lot number being used (this is found on the outside of the kit box).
    4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
    5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
    6. State the sample type and describe the sample preparation steps if applicable.

    X射线造影剂成分


    产品编号 产品名称 产品规格 产品等级 产品价格
    091-05551 Iopamidol 碘帕醇 5g
    099-05552 Iopamidol 碘帕醇 25g
    022-00425 Barium Sulfate 硫酸钡 500g

    X射线造影剂成分


    ◆碘帕醇(Iopamidol)

    CAS No. 60166-93-0

    C17H22I3N3O8=777.09

    纯度:98.0+%(HPLC)

    可溶性溶剂:水

    保存条件:冷藏

    用途(作用):构成元素的碘有很高的X射线吸收能力。

    X射线造影剂成分

    ◆硫酸钡(Barium Sulfate)


    CAS No. 7727-43-7

    BaSO4=233.39

    用途(作用):由于对射线吸收能力强,溶解度小,对人体无害,所以是理想的X射线造影剂

    相关资料详情请查看:http:///pdf/show/80.html

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