康宁corning 3492 TW COL,24MM,3.0UM,PTFE,S,IND, Transwell-COL-膜嵌套 24mm直径 3.0um孔径PTFE(聚四氟乙烯)膜 COL表面 灭菌 单个包装 1个/包

康宁corning 3492 TW COL,24MM,3.0UM,PTFE,S,IND,
Transwell-COL-膜嵌套 24mm直径 3.0um孔径PTFE(聚四氟乙烯)膜 COL表面 灭菌 单个包装 1个/包 24包/箱 3962.54

Amresco 硫酸庆大霉素0304

Amresco 硫酸庆大霉素- 0304-10g

英文名:Gentamycin Sulfate;Gentamicin;Ganamycin;Gentalin;Cidomycin

中文名:硫酸庆大霉素;硫酸正泰霉素;庆大霉素

货号:0304-10G

品牌:AMRESCO

保存温度:2-8˚ C

硫酸庆大霉素
用途:本品为氨基糖甙类广谱抗生素,对多种革兰阴性菌及阳性菌都具有抑菌和杀菌作用。对绿脓杆菌、产气杆菌、肺炎杆菌、沙门氏菌属、大肠杆菌及变形杆菌等革兰阴性菌和金葡菌等作用较强。
本品的作用机制是与细菌核糖蛋白体亚单位上的特异性蛋白牢固结合,干扰核糖蛋白体功能,阻止蛋白质合成,并引起翻译信使核糖核酸(mRNA)上密码的错误而合成无功能蛋白质。

Amresco相关资料如下:
Product: GENTAMYCIN SULFATE
Grade: TISSUE CULTURE GRADE
Appearance: White to off-white powder
Mol. Formula: Not Applicable
Mol. Weight: Not Applicable
Code: 0304
CAS:1405-41-0
Potency (Dry Basis) (mcg/mg) …………………….≥590
Moisture (LOD) (%) …………………………….≤18.0
Identification ………………………………….PASS
pH (4%, Water) @25°C ……………………… 3.5 – 5.5
Specific Rotation (degrees) …………….+107.0 – +121.0
Residue after Ignition ) (%) …………………….≤1.0
This material can be reconstituted in water and generally the recommended working concentration is 15 ug/ml.  This however can depend on the application. 
品名
产地
货号
规格
单价
备注
Gentamycin Sulfate硫酸庆大霉素
Amresco
0304
1G
询价
现货
Gentamycin Sulfate硫酸庆大霉素
Amresco
0304
5G
询价
现货
硫酸庆大霉素

分子式:C21H43N5O7.H2SO4                 分子量:575.67
物理性状及指标:
外观:……………………白色或类白色粉末
熔点:……………………218-237 °C
溶解性:…………………在水(115 mg/ml,25 °C)中易溶,在乙醇(<1 mg/ml,25 °C)、丙酮、三氯甲烷或乙醚中不溶
干燥失重:………………≤18.0%
含量:……………………≥590IU
IC50:……………………半数致死剂量 (LD50) 经口 – 大鼠 – > 5,000 mg
用途及描述:科研试剂,广泛应用于分子生物学,药理学等科研方面。硫酸庆大霉素为氨基糖苷类抗生素。对各种革兰阴性细菌及革兰阳性细菌都有良好的抗菌作用,对各种肠杆菌科细菌如大肠埃希菌、克雷伯菌属、变形杆菌属、沙门菌属、志贺菌属、肠杆菌属、沙雷菌属及铜绿假单胞菌等有良好作用。奈瑟菌属和流感嗜血杆菌对本品中度敏感。对布鲁菌属、鼠疫杆菌、不动杆菌属、胎儿弯曲菌也有一定作用。对葡萄球菌属(包括金黄色葡萄球菌和凝固酶阴性葡萄球菌)中的甲氧西林敏感菌株约80%有良好抗菌作用,但甲氧西林耐药株则对本品多数耐药。对链球菌属和肺炎链球菌的作用较差,肠球菌属则对本品大多耐药。本品与β内酰胺类合用时,多数可获得协同抗菌作用。本品的作用机制是与细菌核糖体30S亚单位结合,抑制细菌蛋白质的合成。
储存条件:室温,避光防潮密闭干燥。

无DMSO细胞冻存液


产品编号 产品名称 产品规格 产品等级 产品价格
CPL-A1 CryoScarless DMSO-Free 
 无DMSO细胞冻存液
100 ml

无DMSO细胞冻存液无DMSO细胞冻存液

CryoScarless DMSO-Free

 


CryoScarless DMSO-Free 是一种无血清、无DMSO 的冻存液,用于细胞的长期低温(-80℃或液氮)保存。

 


◆特点•优势

无DMSO细胞冻存液

●    无血清、无DMSO

●    高细胞活力、无DMSO 和无血清来源蛋白产生细胞毒性的风险

●    90% 以上的细胞冻融后仍具有一致性和高细胞活力

●    可维持干细胞分化能力

●    无细菌、真菌和支原体污染

●    保质期长,4℃下可保存2 年





◆案例•应用


对比数据


无DMSO细胞冻存液

               大鼠间充质干细胞冻存复苏后的分化情况

  

        结果显示大鼠间充质干细胞用CryoScarless DMSOFree冻存后依然保持良好的细胞全能性

无DMSO细胞冻存液

        鼠间充质干细胞冻存复苏后细胞活力检测

 

用CryoScarless DMSO-Free 冻存的细胞活力明显高于含10%DMSO的细胞冻存液。

0h:复苏后立即检测6h:细胞粘附之后

应用

Cell type Cell viability(%)
L929 97.5±1.2
MG63 93.1±2.3
HT1080 90.2±4.3
Colon26 92.3±2.3
B16F1 94.2±0.6
KB 91.8±0.9
Caco2 93.7±1.9
MC3T3 94.4±0.5
JurkatE6-1 88.9±0.4
HUVEC 89.9±0.4
HCAEC 90.1±1.6
MEF 94.4±0.8
hACh 93.5±0.7

* 细胞用CryoScarless DMSO-Free 冻存液-80℃保存3个月,复苏细胞24小时后进行活力检测。

实验操作步骤 


1. 离心培养细胞,去上清;

2. 用CryoScarless DMSO-Free 冻存液重悬细胞(1mL含5×105-106个细胞),每管分装1mL;

3. -80℃低温冻存;

  * 为了长期保存,可以置于液氮罐中。

4. 取出细胞,37℃恒温水浴锅快速溶解,立即用10mL的适当培养基稀释,轻轻混匀。

Thermo官网Thermo Fisher赛默飞中国代理商

Thermo官网 Thermo Fisher赛默飞中国代理商
赛默飞世尔科技是全球科学服务领域的领导者,致力于帮助客户使世界更健康,更清洁,更安全。我们努力为客户提供最为便捷的采购方案,为科研的飞速发展不断地改进工艺技术,提升客户价值,帮助股东提高收益,为员工创造良好的发展空间。公司拥有两大主要品牌Thermo Scientific 和 Fisher Scientific。
上海金畔生物科技有限公司是一家销售Thermo Fisher赛默飞全线产品的中国代理公司。
固话总机:021-50837765
订货热线:15221999938
qq号:2743691513 1042640511
微信号:jinpanbio
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn

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日本关东化学代理商  日本KANTO化学中国代理商

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上海金畔生物科技有限公司是一家销售日本关东化学试剂的中国代理公司。
固话总机:021-50837765
订货热线:15221999938
qq号:2743691513 1042640511
微信号:jinpanbio
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn

关东化学株式会社是日本著名的综合试剂厂家它的高质量产品在国际上享有极高的声誉。不仅在中国国内 多年来在日本国内、亚洲地区、美国、欧洲等地都深受厚爱。为了保证所有试剂产品的质量 关东化学株式会社的全工厂都取得了ISO 9001和ISO/IEC 17025质量管理体系认证 建立了严格的品质管理体系。1998年 全工厂又在日本试剂行业中率先取得了ISO 14001环境管理体系认证。关东化学株式会社不仅是一家拥有先端技术的生产企业也是一家重视环境安全以及人类社会健康和谐的可信赖企业。

无论是一般试剂还是特殊试剂、从零售到大量批发 我公司都会竭尽全力地为您提供优质服务、满足您的需求。

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上海金畔生物科技有限公司是一家销售日本关东化学试剂的中国代理公司。
固话总机:021-50837765
订货热线:15221999938
qq号:2743691513 1042640511
微信号:jinpanbio
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com
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上海金畔同时代理日本日水培养基系列 在生命科学领域,康宁为业界提供了丰富的实验室和生物医药生产研发的产品。上海金畔生物代理Nissui(日水)培养基产品,在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Nissui(日水)培养基高品质的产品和服务,欢迎广大新老客户来电咨询。 Nissui日水代理,Nissui日水上海代理,Nissui日水北京代理,Nissui日水广州代理

乙酸检测试剂盒[乙酸激酶法] Acetic Acid (Acetate Kinase Manual Format) 货号:K-ACETRM Megazyme中文站

乙酸检测试剂盒[乙酸激酶法]

英文名:Acetic Acid (Acetate Kinase Manual Format)

货号:K-ACETRM

规格:72 assays (manual) / 720 assays (microplate)

分析物意义: 常见食品的组分

Megazyme检测试剂盒优点: K-ACETRM 是运用AK和磷酸乙酰转移酶的新型、快速的手工检测试剂盒。试剂稳定

K-ACETAK (自动) 是一种以乙酸激酶(AK)为基础的,新型、稳定、快速的检测试剂盒,具有良好的线性。

This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials. Content:72 assays

乙酸检测试剂盒[乙酸激酶法]

Manual format UV-method for the determination of Acetic Acid 
in foodstuffs, beverages and other materials

Principle:
                         (acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP

                          (phosphotransacetylase)
(2) Acetyl-phosphate + CoA → acetyl-CoA + Pi

           (pyruvate kinase)
(3) ADP + PEP → ATP + pyruvate

                       (D-lactate dehydrogenase)
(4) Pyruvate + NADH + H+ → D-lactic acid + NAD+

Kit size:                            72 assays (manual) / 720 (microplate)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 4 min
Detection limit:                 0.063 mg/L
Application examples: 
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, 
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products
(and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper
(and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:         Improved method

Advantages

  • Improved assay format (only two absorbance readings required)
     
  • All reagents stable for > 2 years after preparation
     
  • PVP incorporated to prevent tannin inhibition
     
  • Very rapid reaction (~ 4 min)
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Very competitive price (cost per test)
     
  • Suitable for Manual and Microplate formats

Q1. Is the acetic acid kit specific for acetate?

Propionate may react more slowly than acetate.

Q2. Is the K-ACETRM Assay Kit suitable for measurement using cell culture media samples?

Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit.  It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed but dilution (if required) in distilled water. 

Q3. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q4. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q5. Does the decolourising preparation remove some VA during the process?

No, however the sample preparation process can be tested by adding a known amount of acetic acid standard and assessing the recovery of this. 

Q6. Can acetic acid be measured in culture/fermentation media?

Acetic acid in liquid cell culture media/supernatants or fermentation samples can be determined without any sample treatment (except clarification by centrifugation or filtration) and appropriate dilution in distilled water. 

Q7. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q8. What are the major the differences between the various acetic acid test kits?

Megazyme produces 4 acetic acid test kits:
K-ACET: uses the traditional ACS reaction.  Manual format for use with spectrophotometers.
K-ACETAF: uses the traditional ACS reaction.  Automated format for use with auto-analysers.
K-ACETAK: uses the more recently developed and more rapid acetate kinase reaction.  Automated format for use with auto-analysers.
K-ACETRM: uses the more recently developed and more rapid acetate kinase reaction.  Manual format for use with spectrophotometers. 

Q9. Which acetic acid kit is recommended for a 96-well microplate format?

Auto-analysers use ~ 0.315 mL reaction volumes and pathlengths between 4-8 mm which is similar to a standard 96-well microplate where a 0.315 mL reaction volume would give a pathlength of ~ 6-7 mm.  Therefore K-ACETAK or K-ACETAF can be used directly in a 96-well microplate format with minimal assay optimisation.
If preferred, K-ACET or K-ACETRM may also be easily converted for use in a 96-well microplate format.  Basically, the assay volumes for the cuvette format must be reduced approximately 10-fold for use in a 96-well microplate.  However, some assay optimisation may be required (e.g. increased enzyme concentration etc.) and unlike the cuvette which has a set pathlength of 1 cm, the pathlength in the microplate is dependent upon the volume of liquid in the well.  Therefore to enable the calculation of the amount of analyte in the samples from tests performed in the microplate format one of the following must be done:

  1. The easiest method is to use a microplate reader that has a pathlength conversion capability (i.e. the microplate reader can detect the pathlength of each well and convert the individual readings to a 1 cm pathlength).  This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm pathlength) and the 96-well microplate format and use these results to obtain a     mean conversion factor between the cuvette values and the microplate values.


Acetic Acid Kit Recommendation For Microplate Format:
Either K-ACETRM or K-ACETAK is recommended for use in a 96-well microplate format and the main advantages / disadvantages are described below:
K-ACETRM:
The assay volumes of this kit should be reduced by 10-fold for use in a 96-well microplate format (some assay optimisation may be required, e.g. increased enzyme concentration etc.).
The calculation of results is achieved as outlined above in either of points 1, 2 or 3. 

Q10. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q11. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q12. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q13. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q14. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q15. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

 

exo-α-Sialidase (Salmonella typhimurium) exo-α-Sialidase (Salmonella typhimurium) 货号:E-SIALST Megazyme中文站

exo-α-Sialidase (Salmonella typhimurium)

英文名:exo-α-Sialidase (Salmonella typhimurium)

货号:E-SIALST

规格:250 Units

High purity recombinant exo-alpha-Sialidase (S. Typhimurium) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

EC 3.2.1.18
CAZY Family: GH33
Recombinant. From Salmonella typhimurium. In solution (Tris.HCl / NaCl / EDTA). 
Hydrolysis of unbranched, non-reducing terminal α-2,3-linked >> α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates.
Specific activity: ~ 750 U/mg (37oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid)
Store at 4oC.  

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二乙酰壳二糖 Diacetyl-Chiotobiose – 30mg 货号:O-CHI2 Megazyme中文站

二乙酰壳二糖

英文名:Diacetyl-Chiotobiose – 30mg

货号:O-CHI2

规格:30 mg

CAS: 35061-50-8
Molecular Formula: C16H28N2O11
Molecular Weight: 424.4
Purity: > 95%

High purity Diacetyl-chitobiose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Prepared from chitin.

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