磷酸酶(碱性)(大肠杆菌) Phosphatase (Alkaline) (E. coli) 货号:E-ALPEC Megazyme中文站

磷酸酶(碱性)(大肠杆菌)

英文名:Phosphatase (Alkaline) (E. coli)

货号:E-ALPEC

规格:400 Units

High purity recombinant Alkaline phosphatase (E. coli) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.1.3.1

Orthophosphoric-monoester phosphohydrolase (alkaline optimum).

Recombinant from E. coli. 
In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4.

Specific activity: ~ 10 U/mg (37oC, pH 10.4, 4-Nitrophenyl phosphate).

Stable at 4oC for > 2 years.

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harlan teklad动物饲料官网 TD.88137 饮食诱导肥胖(DIO) Adjusted calories diet (42% from fat)

饮食诱导肥胖Envigo

harlan teklad动物饲料官网 TD.88137 饮食诱导肥胖(DIO) Adjusted calories diet (42% from fat)

用于诱导肥胖和肥胖相关并发症(如糖尿病和代谢综合征)的纯化高脂饮食通常有40-60%的能量来源于脂肪。下面的饮食表总结了一些特克拉德定制研究饮食的相关饮食特征,这些研究饮食通常用于啮齿类动物模型。

泰克乐还为其他物种,包括猪、灵长类动物和狗创造高脂肪饮食。联系我们讨论使用这些饮食或更好地满足您的需要。

通常使用的饮食诱导肥胖(DIO)技术啮齿动物饮食中含有55-60%的热量来自脂肪。
饮食特征 TD.06414 备好 TD.93075 面团 TD.07011 球团
千卡/克 5.1 4.8
脂肪,%千卡 60 55
脂肪来源,
按重量计百分比
31%猪油
3%豆油
27.4%蔬菜酥油
1.6%玉米油
脂肪酸谱,
总脂肪百分比
37%饱和
47%单不饱和
16%多不饱和
28%饱和,30%反式
28%单不饱和(顺式)
14%多不饱和
蔗糖
按重量计百分比
12.1 9.6
注记 60F10S海报数据
与D 12492比较
反式脂肪
示例修改 TD.08500椰子油
TD.09766乳脂
成分匹配,
低脂控制饮食*
TD.06416(35%蔗糖)
TD.08806(11%蔗糖)
TD.93074(21%蔗糖)
TD.120651(7%蔗糖)
参考文献
大鼠

大鼠

*控制饮食可以通过几种方式来设计,这取决于研究人员相对于高脂肪饮食想要改变什么特征。这些只是几个例子。

通常使用的饮食诱导肥胖(DIO)技术啮齿动物饮食中含有40-45%的热量来自脂肪。
饮食特征 TD.06415 TD.08811 TD.88137 备好 TD.95217
千卡/克 4.6 4.7 4.5 4.3
脂肪,%千卡 45 45 42 40
脂肪来源,
按重量计百分比
19.5%猪油
3%豆油
21%乳脂
2%豆油
21%乳脂 10.6%蔬菜酥油
4%乳脂
4%豆油
脂肪酸谱,
总脂肪百分比
36%饱和
46%单不饱和
18%多不饱和
61%饱和
31%单不饱和
8%多不饱和
62%饱和
27%单不饱和
5%多不饱和
34%饱和,18%反式
29%单不饱和(顺式)
19%多不饱和
蔗糖
按重量计百分比
22.8 36.8 34.5 15.8
注记 与D 12451比较 45F30S海报数据 “西餐”
添加胆固醇
反式脂肪
示例修改 TD.110716乳脂
TD.10670无染料
TD.130784猪油
TD.120438无染料
TD.07201猪油
TD.00573椰子油,不含胆固醇
TD.09682蓝色染料
TD.07734绿色染料
成分匹配,
低脂控制饮食*
TD.06416(35%蔗糖)
TD.110675(18%蔗糖)
TD.120455(6%蔗糖,抗性淀粉)
TD.120724(14%蔗糖)
TD.05230(34%蔗糖)
TD.08485(12%蔗糖)
TD.06101(6%蔗糖)
参考文献
大鼠

大鼠

大鼠

大鼠
全塌

从脂肪中摄取55-60%卡路里的食物TD.06414TD.93075通常用于诱发啮齿动物肥胖。虽然与典型的人类脂肪摄入相比,这些饮食被认为是极端的,但在大多数啮齿类动物中,这些饮食能有效地启动快速增重。由于脂肪含量较高,碳水化合物的摄入空间较小,因此碳水化合物(特别是蔗糖)含量相对较低。如果你对高脂肪和高碳水化合物感兴趣,那就看看脂肪中含有40-45%卡路里的饮食(通常被称为西方饮食)。

随着脂肪水平的增加,球团的质量(耐久性)经常受到损害。一些高脂肪配方只有在非颗粒形式,或需要特定的碳水化合物,麦芽糊精,用于球团。根据使用的脂肪和碳水化合物来源,非颗粒形式可能是致密和碎屑,面团样,或糊状。虽然使用非颗粒饮食更具有挑战性,但许多研究人员仍在使用非颗粒饮食来建立饮食诱导的肥胖模型,因为这些研究人员怀疑,较软的饮食形式可能会促进肥胖的发展。

从脂肪中摄取40-45%卡路里的饮食,如TD.95217TD.88137TD.06415,和TD.08811,代表了另一种流行的饮食模式-诱发肥胖的工作。这些食物的蔗糖含量是高脂肪饮食的两倍或三倍。高水平的简单碳水化合物,如蔗糖和果糖,可能有助于促进高甘油三酯血症,胰岛素抵抗和脂肪肝。高蔗糖、高饱和或反式脂肪的饮食在肥胖和心血管领域常被称为“西方饮食”。一些“西方饮食”有进一步的修改脂肪酸剖面,甚至特定的维生素和矿物质调整,以更紧密地匹配西方饮食模式。有关特定脂肪酸的修改,请参阅脂肪/脂肪调节饮食一页。

许多用于诱导啮齿动物肥胖的饮食可以用于增强糖尿病相关表型,如胰岛素抵抗和葡萄糖不耐受。然而,空腹高血糖特征的糖尿病(葡萄糖>200 mg/dL)是罕见的饮食方法。预先喂食高脂饮食,诱导一定程度的肥胖和胰岛素抵抗,然后给予小剂量链脲佐菌素(STZ)可能是一个有效的方法,如果公开高血糖是需要。

除了不同类型的碳水化合物外,还有许多不同水平和类型的脂肪,从蔗糖(高精制,简单消化)到玉米淀粉(精制,但更复杂),再到抗性淀粉(精制,但不能完全消化)。一个非常基本的净化控制饮食将是AIN-93M (TD.94048) 或AIN-93g(TD.94045)AIN-93日粮中蔗糖含量在10%左右,脂肪来源于具有健康脂肪酸的大豆油。更多的控制特定DIO饮食的例子可以在上表中找到。

许多研究人员选择将高脂肪喂养的动物与食用天然成分谷物的动物(也称为标准饮食或周食)进行比较。这些饮食在营养来源和水平以及非营养因素(如植酸盐或植物雌激素)存在的情况下存在差异。根据您的主要比较,它可能适合作为您的对照/参考组谷物基础的饮食。然而,这样的比较限制了对饮食模式和特定饮食成分的推断。

Diet induced obesity | Envigo

Purified high fat diets used to induce obesity and obesity-related complications such as diabetes and metabolic syndrome typically have 40-60% of energy derived from fat. The diet tables below summarize relevant diet features for several Teklad custom research diets commonly used in rodent models.

Teklad also creates high-fat diets for other species, including pigs, primates, and dogs. Contact us to discuss the use of these diets or one that better meets your needs.

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 55-60% of calories from fat
Diet features TD.06414stocked TD.93075dough TD.07011pellet
Kcal/g 5.1 4.8
Fat, % Kcal 60 55
Fat Sources,
% by weight
31% lard
3% soybean oil
27.4% vegetable shortening
1.6% corn oil
Fatty acid profile,
% total fat
37% saturated
47% monounsaturated
16% polyunsaturated
28% saturated, 30% trans
28% monounsaturated (cis)
14% polyunsaturated (cis)
Sucrose,
% by weight
12.1 9.6
Notes 60F10S poster data
Compare to D12492
Trans fat
Example modifications TD.08500coconut oil
TD.09766 milk fat
Ingredient matched,
low fat control diets*
TD.06416 (35% sucrose)
TD.08806 (11% sucrose)
TD.93074 (21% sucrose)
TD.120651 (7% sucrose)
References Mouse
Rat
Mouse
Rat

*Control diets can be designed in several ways, depending on what features the researcher wants to modify relative to the high-fat diet. These are just a few examples.

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 40-45% of calories from fat
Diet features TD.06415 TD.08811 TD.88137stocked TD.95217
Kcal/g 4.6 4.7 4.5 4.3
Fat, % Kcal 45 45 42 40
Fat sources,
% by weight
19.5% lard
3% soybean oil
21% milk fat
2% soybean oil
21% milk fat 10.6% vegetable shortening
4% milk fat
4% soybean oil
Fatty acid profile,
% total fat
36% saturated
46% monounsaturated
18% polyunsaturated
61% saturated
31% monounsaturated
8% polyunsaturated
62% saturated
27% monounsaturated
5% polyunsaturated
34% saturated, 18% trans
29% monounsaturated (cis)
19% polyunsaturated (cis)
Sucrose,
% by weight
22.8 36.8 34.5 15.8
Notes Compare to D12451 45F30S poster data “Western Diet”
Cholesterol added
Trans fat
Example modifications TD.110716 milk fat
TD.10670 no dye
TD.130784 lard
TD.120438 no dye
TD.07201 lard
TD.00573 h-coconut oil, no cholesterol
TD.09682 blue dye
TD.07734 green dye
Ingredient matched,
low fat control diets*
TD.06416 (35% sucrose)
TD.110675 (18% sucrose)
TD.120455 (6% sucrose, resistant starch)
TD.120724 (14% sucrose)
TD.05230 (34% sucrose)
TD.08485 (12% sucrose)
TD.06101 (6% sucrose)
References Mouse
Rat
Mouse
Rat
Mouse
Rat
Mouse
Rat
Collapse All Tabs

Diets with 55-60% of calories from fat like TD.06414 and TD.93075 are commonly used for inducing obesity in rodents. While considered extreme compared to typical human fat consumption, these diets are effective in initiating rapid weight gain in most rodents. With higher fat content there is less room for carbohydrate, thus the carbohydrate (particularly sucrose) amount is relatively low compared to other obesity inducing diets. If you are interested in high fat and high carbohydrate, look at diets with 40-45% of calories from fat (often referred to as western diets).

As the fat level increases, pellet quality (durability) is often compromised. Some higher fat formulas are available only in non-pelleted form or require specific carbohydrate, maltodextrin, for pelleting. Depending on the fat and carbohydrate sources used, the non-pelleted form could be dense and crumbly, dough-like, or paste-like. Though a little more challenging to work with, non-pelleted diet is still used by many researchers for diet-induced obesity models as these researchers suspect the softer form may enhance obesity development.

Diets with 40-45% of calories from fat, like TD.95217TD.88137TD.06415, and TD.08811, represent another popular diet pattern for diet-induced obesity work. These diets have double or triple the amount of sucrose found in higher fat diets. High levels of simple carbohydrate like sucrose and fructose may help to promote hypertriglyceridemia, insulin resistance, and fatty liver. Diets with a pattern of high sucrose and high saturated or trans fat are often referred to as “Western Diets” in obesity and cardiovascular fields. Some “Western Diets” have further modifications to the fatty acid profile or even specific vitamin and minerals adjustments to be even more closely matched to a Western Diet pattern. For specific fatty acid modifications, see examples on our fat/lipid adjusted diets page.

Many of the same diets used for inducing obesity in rodents can be used to enhance diabetes related phenotypes like insulin resistance and glucose intolerance. However, fasting hyperglycemia characteristic of diabetes (glucose > 200 mg/dL) is uncommon with a diet only approach. Pre-feeding a high fat diet to induce a certain level of obesity and insulin resistance and then giving low-dose streptozotocin (STZ) may be an effective approach if overt hyperglycemia is desired.

There are many options with different levels and types of fat in addition to different types of carbohydrate ranging from sucrose (highly refined, simple digestion) to corn starch (refined, but more complex) to resistant starch (refined, but not fully digestible). A very basic purified control diet would be AIN-93M (TD.94048) or AIN-93G (TD.94045). AIN-93 diets have a moderate amount of sucrose at ~10%, and fat is from soybean oil with a healthy fatty acid profile. Additional examples of controls for specific DIO diets can be found in the above tables.

Many researchers choose to compare their high fat fed animals to animals fed a natural ingredient, grain-based diet (also referred to as standard diets or chow). These diets differ in the source and level of nutrients as well as in the presence of non-nutritive factors (such as phytates or phytoestrogens). Depending on what your main comparisons are, it may be suitable to have a grain-based diet as your control/reference group. However, making such comparisons limits inferences to dietary patterns versus a specific dietary component.

61-a-D-半乳-甘露二糖+岑糖 61-A-D-Galactosyl-Mannotriose -20mg 货号:O-GM3 Megazyme中文站

61-a-D-半乳-甘露二糖+岑糖

英文名:61-A-D-Galactosyl-Mannotriose -20mg

货号:O-GM3

规格:20 mg

CAS: 82220-78-8
Molecular Formula: C24H42O21
Molecular Weight: 666.6
Purity: > 95%

High purity 61-alpha-D-Galactosyl-mannotriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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日本和光wako 164-21655 聚乙烯硫酸钾溶液 PVSK胶体滴定液 N/400

日本和光wako 164-21655 聚乙烯硫酸钾溶液 PVSK胶体滴定液 N/400

日本和光纯药试剂(wako)   N/400聚乙烯硫酸钾  500ml/瓶  聚乙烯硫酸钾(原装)是WAKO产品之一,WAKO(和光纯药)是世界五大实验室化学试剂供应商之一

 

 

164-21655-聚乙烯硫酸钾(PVSK)滴定液(N /400)日本和光Wako进口

日本和光纯药164-21655 聚乙烯硫酸钾溶液 PVSK胶体滴定液 N/400

聚乙烯硫酸钾(PVSK)
CAS号:26837-42-3
产地:日本和光纯药 WAKO
包装:500ML/瓶
【用途】聚乙烯硫酸钾Potassium Polyvinyl Sulfate(PVSK)是一种简单、快速、准确的测定高分子聚合物的阴离子胶质滴定用液。利用直接滴定,进行阳离子高分子化合物的定量。于水中可形成带负电的胶体颗粒,甲基乙二醇壳聚糖滴定液-Methyl Glycol Chitosan(MGC)在酸性水溶液中带正电,利用电性中和的原理,负电胶体颗粒的会与正电胶体颗粒反应达到电性中和,在适当的pH条件下,此两种胶体可完全反应形成不溶性沉淀物,使用甲苯胺蓝Toluidine Blue(TB)为指示剂,与正电性胶体混合时,两者之间没有反应,TB维持蓝色;当悬浮液中胶体带负电时,TB会吸附于胶体表面而现紫色,滴定时,正电胶体可直接以PVSK溶液为滴定液,TB为指示剂进行滴定;对于负电荷胶体,可以直接以MGC滴定,或加入已知的过量的正电胶体,再以PVSK溶液对剩余的正电胶体进行反滴定。阳离子或碱性高分子的定量(容量分析)。

 

Potassium Polyvinyl Sulfate Titration Solution (N/400)

聚乙烯硫酸钾滴定液(N /400)

品牌:Wako
品牌中文简称:和光纯药
CAS No.:26837-42-3
储存条件:室温
纯度:
产品编号 等级 规格 零售价(RMB)
164-21655 for Colloidal Titration 500 ml 咨询

 

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

 

* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系宝柏·中国客服

产品描述

胶体滴定(colloid titration) 是一种简单、快速、准确的测定高分子聚合物电荷的方法。

聚乙烯硫酸钾(Potassium Polyvinyl Sulfate,PVSK)于水中可形成带负电的胶体颗粒,(Glycol Chitosan,GC)或Methyl Glycol Chitosan(MGC)在酸性水溶液中带正电,利用电性中和的原理,负电胶体颗粒的会与正电胶体颗粒反应达到电性中和。

在适当的pH条件下,此两种胶体可完全反应形成不溶性沉淀物。使用甲苯胺蓝(Toluidine Blue,TB)为指示剂,与正电性胶体混合时,两者之间没有反应,TB维持蓝色;当悬浮液中胶体带负电时,TB会吸附于胶体表面而现紫色。

滴定时,正电胶体可直接以PVSK溶液为滴定液,TB为指示剂进行滴定;对于负电荷胶体,可以直接以乙二醇壳聚糖或甲基乙二醇壳聚糖滴定,或加入已知的过量的正电胶体,再以PVSK溶液对剩余的正电胶体进行反滴定。

 

应用:主要用于聚合物分析,如测定聚合物电荷;测定聚合物取代度等。

 

产品编号

产品名

中文名

包装

CAS

阴离子试剂

162-03071 Potassium Polyvinyl Sulfate 聚乙烯硫酸钾(PVSK) 10g 26837-42-3

阳离子试剂

072-05045 Glycol Chitosan Titration Solution (N/200) 乙二醇壳聚糖滴定液(N/200) 500ml 39280-86-9
072-01581 Glycol Chitosan 乙二醇壳聚糖 10g 39280-86-9
137-14655 Methyl Glycol Chitosan  Titration Solution(N/200) 甲基乙二醇壳聚糖滴定液(N/200) 500ml 88650-88-8
134-04731 Methyl Glycol Chitosan 甲基乙二醇壳聚糖 10g 88650-88-8
161-14695 0.0025N Poly(diallyldimethylammonium    Chloride) Solution 聚二甲基二烯丙基氯化铵滴定液(0.0025N) 500ml 26062-79-3

指示液

205-05811 Toluidine Blue Indicator Solution 甲苯胺蓝指示剂 50ml 92-31-9

PVSK标定试剂

080-06681 HexadecylpyridiniumChlorideMonohydrate 氯化十六烷基嘧啶 10g 6004-24-6
086-06683 Hexadecylpyridinium Chloride  Monohydrate 氯化十六烷基吡啶 100g 6004-24-6

尿/氨[快速]检测试剂盒 Urea/Ammonia (Rapid) Assay Kit 货号:K-URAMR Megazyme中文站

尿/氨[快速]检测试剂盒

英文名:Urea/Ammonia (Rapid) Assay Kit

货号:K-URAMR

规格:100 assays (50 of each) per kit

The Urea/Ammonia (Rapid) test kit is suitable for the specific and rapid measurement and analysis of urea and ammonia in water, beverages, milk and food products.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

UV-method for the determination of Urea and Ammonia in
foodstuffs, beverages and other materials

Principle:
(urease)
(1) Urea + H2O → 2NH3 + CO2

(microbial glutamate dehydrogenase)
(2) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP+
+ H2O

Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 8 min
Detection limit: 0.13 mg/L (urea)
0.07 mg/L (ammonia)
Application examples:
Wine, grape juice, must, fruit juices, soft drinks, milk, cheese, meat,
processed meat, bakery products, seafood, fertilizers, feed,
pharmaceuticals, cosmetics, water (e.g. swimming-pool water), Kjeldahl
analysis, paper (and cardboard) and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by NEN and MEBAK

Advantages

  • Very rapid reaction due to use of uninhibited glutamate dehydrogenase
  • Enzymes supplied as stable Suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability

 

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氯吡多,氯羟吡啶标准品


产品编号 产品名称 产品规格 产品等级 产品价格
038-19931 Clopidol Standard 
氯吡多,氯羟吡啶标准品
200mg for High Performance Liquid Chromatography

氯吡多,氯羟吡啶标准品

  氯羟吡啶属吡啶类化合物,具有广泛地抗球虫作用。可用于禽、兔球虫病。氯羟吡啶对球虫的作用峰期主要在于孢子发育阶段,能使子孢子在上皮细胞内停止发育长达 60 天。最近还发现对第 2 代裂殖生殖,配子生殖和孢子形成均有抑制作用。由于氯羟吡啶对球虫仅是抑制作用,停药后,子孢子即能重新发育成长。氯羟吡啶能使宿主对球虫的免疫力明显降低。

GB 29700-2013  食品安全国家标准 牛奶中氯羟吡啶残留量的测定  气相色谱-质谱法。本标准规定了牛奶中氯羟吡啶残留量检测的制样和气相色谱-质谱的测定方法。本标准适用于牛奶中氯羟吡啶残留量的检测。

产品信息


货号

中文品名

英文品名

CAS No.

规格

038-19931

氯吡多标准品

Clopidol Standard

2971-90-6

200   mg

烯烃的羧基化


产品编号 产品名称 产品规格 产品等级 产品价格
162-24711 Palladium(Ⅱ) Chloride 
氯化钯
1G for Organic Synthesis
168-24713 Palladium(Ⅱ) Chloride
氯化钯
5g for Organic Synthesis
160-24712 Palladium(Ⅱ) Chloride
氯化钯
25g for Organic Synthesis
039-04152 Copper(I) Chloride
氯化铜
25g for Organic Synthesis
031-04151 Copper(I) Chloride
氯化铜
100g for Organic Synthesis
033-04155 Copper(I) Chloride
氯化铜
100g for Organic Synthesis

烯烃的羧基化


◆优点特色

● Wacker氧化

● 一般使用PdCl2或CuCl2,在酸性条件下末端的烯烃被氧化

● 反应条件十分温和,内部的烯烃几乎不被氧化

● 反应体系内会生成盐酸

● 水以外的亲核试剂存在下发生的反应称为Wacker型氧化,常用作分子内环化反应

反应例子【a】【b】


烯烃的羧基化

产品编号

产品名称

容量

CAS

162-24711

氯化钯Palladium(Ⅱ) Chloride

1g

7647-10-1

168-24713

5g

160-24712

25g

039-04152

氯化铜Copper(I)   Chloride

25g

7758-89-6

031-04151

100g

033-04155

500g