日本ATTO株式会社官网电泳仪电泳槽 垂直电泳仪AE-6531

日本ATTO株式会社  www.atto.co.jp

日本ATTO株式会社官网电泳仪电泳槽 垂直电泳仪AE-6531

日本ATTO株式会社  www.atto.co.jp
日本ATTOAE-3750干胶仪
日本ATTOAE-3750干胶仪
日本ATTO干胶仪AE-3711 mini
日本ATTO干胶仪AE-3711 mini
日本ATTO干胶仪AE-3750
日本ATTO干胶仪AE-3750
日本ATTO水平半干转印仪(蛋白,核酸通用型)AE-6687
日本ATTO水平半干转印仪(蛋白,核酸通用型)AE-6687
日本ATTO水平半干转印仪AE-6685P
日本ATTO水平半干转印仪AE-6685P
日本ATTO温控测序电泳槽AE-6141 Mni-Quencer
日本ATTO温控测序电泳槽AE-6141 Mni-Quencer
日本ATTO测序电泳槽AE-6155
日本ATTO测序电泳槽AE-6155询价
日本ATTO水平琼脂电泳槽AE-6111
日本ATTO水平琼脂电泳槽AE-6111
日本ATTO等电聚焦电泳仪AE-6541
日本ATTO等电聚焦电泳仪AE-6541
日本ATTO多胶垂直电泳仪AE-7341
日本ATTO多胶垂直电泳仪AE-7341
日本ATTO垂直电泳仪AE-6531
日本ATTO垂直电泳仪AE-6531
日本ATTO垂直电泳仪AE-6531
日本ATTO垂直电泳仪AE-6531
日本ATTO制备电泳AE-6750S
日本ATTO制备电泳AE-6750S
日本ATTOAE-6450垂直电泳槽
日本ATTOAE-6450垂直电泳槽

日本ATTO株式会社成立于1964年,是日本最著名的电泳仪器及生物仪器制造商,主要产品包括:各类小型电泳槽,电源,转印槽,干胶仪,胶染色仪,制备电泳,等电电泳,生物发光检测仪器。ATTO的小型电泳槽做工精致,电源精巧,进入国内两年,已深得市场厚爱。
Back in the 1960s, scholars in the field of biochemistry were hard pressed to find the types of sophisticated equipment they needed for their pioneering research. Atto Corporation was established as a specialist manufacturer in 1964 to meet their demands. The company founders had every confidence that the field of biochemistry was destined to grow. In the four decades since, Atto has invested substantial resources and money in research and development under the guidance of leading biochemists.

Since our establishment, we have continued to develop, manufacture, and sell biological macromolecules and analytical products such as chromatographs and electrophoresis apparatus. Over the last decade we have also developed and marketed a luminescence device for the research of gene expression.

Our name Atto comes from “atto,” an incredibly small unit equal to 1 x 10-18, or one-quintillionth. Modern scientists and technologists now hope to delve deeper into exploratory research on the “atto” scale. This is a field Atto is poised to enter.

In recent years, the Japanese government has begun to promote joint research between universities and enterprises. However, Atto has been engaged in collaboration with universities, and has already achieved several fruitful results to date. One of the results of collaboration won an award from the Ministry of Education, Culture, Sports, Science and Technology in 2001.

Company Profile
Founded: January, 1964
Headquarters: Tokyo, Japan
President: Shigemitsu Yamada
Business:
Development and sales of research support instruments in
biochemistry, molecular biology, and biotechnology fields
Products:
Apparatus for electrophoresis and chromatography,
imaging systems, luminometers, spectrometers, peristalic pumps
and other instruments for biological/biochemical reasearch
Clientele:
1. Universities and research institutes
2. Medical- and food-related companies
3. Overseas universities and research institute

7ml PFA圆底容器 (外径×内径×全高mm:22×17×32H)日本三博特sanplatec

7ml PFA圆底容器 (外径×内径×全高mm:22×17×32H)
产品编号: WEB15268 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

本体尺寸(mm)×外径×内径×高:22Φ×17Φ×32

瓶盖尺寸(mm)×外径×高:27Φ×36

材料

确认材料的耐药性 >> 耐药性检索

        7ml PFA圆底容器 (外径×内径×全高mm:22×17×32H)7ml PFA圆底容器 (外径×内径×全高mm:22×17×32H)产品特征

特点 :瓶子完全由PFA制造 ,耐药品性优越 。金属溶出及少 。作为无金属容 器,适合保管各种分析试料。内部形状为圆形,可以简单取出内容液。 耐热性,耐寒性优越。可在冷冻库冷冻,也可在油浴加热。可可用于广泛的温度领域(-196℃~250℃)。本品为非粘性,适合操作需要剥离或处理高粘度的样品。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/8×19/64(英寸))日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/8×19/64(英寸))
产品编号: WEB10824 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )3/8×19/64(英寸)

规格(外径×内径×厚度):9.53×  7.54×0.995

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/8×19/64(英寸))NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/8×19/64(英寸))产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

6ml PFA圆底容器 (外径×内径×全高(mm):18角×14φ×47H)日本三博特sanplatec

6ml PFA圆底容器 (外径×内径×全高(mm):18角×14φ×47H)
产品编号: WEB15267 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

本体尺寸(mm)×外径×内径×高:18 角 ×14Φ×43

瓶盖尺寸(mm)×外径×高:24Φ×47

材料

确认材料的耐药性 >> 耐药性检索

        6ml PFA圆底容器 (外径×内径×全高(mm):18角×14φ×47H)6ml PFA圆底容器 (外径×内径×全高(mm):18角×14φ×47H)产品特征

特点 :瓶子完全由PFA制造 ,耐药品性优越 。金属溶出及少 。作为无金属容 器,适合保管各种分析试料。内部形状为圆形,可以简单取出内容液。 耐热性,耐寒性优越。可在冷冻库冷冻,也可在油浴加热。可可用于广泛的温度领域(-196℃~250℃)。本品为非粘性,适合操作需要剥离或处理高粘度的样品。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

3ml PFA圆底容器(外径×内径×全高(mm):18角×14φ×27H)日本三博特sanplatec

3ml PFA圆底容器(外径×内径×全高(mm):18角×14φ×27H)
产品编号: WEB15266 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

本体尺寸(mm)×外径×内径×高:18 角 ×14Φ×23

瓶盖尺寸(mm)×外径×高:24Φ×27

材料

确认材料的耐药性 >> 耐药性检索

        3ml PFA圆底容器(外径×内径×全高(mm):18角×14φ×27H)3ml PFA圆底容器(外径×内径×全高(mm):18角×14φ×27H)产品特征

特点 :瓶子完全由PFA制造 ,耐药品性优越 。金属溶出及少 。作为无金属容 器,适合保管各种分析试料。内部形状为圆形,可以简单取出内容液。 耐热性,耐寒性优越。可在冷冻库冷冻,也可在油浴加热。可可用于广泛的温度领域(-196℃~250℃)。本品为非粘性,适合操作需要剥离或处理高粘度的样品。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PCT™ Pre-Crystallization Test蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

Products > Crystallization Screens > PCT Pre-Crystallization Test > PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

Applications

  • Determine the appropriate sample concentration for crystallization screening

Features

  • Conserve sample
  • Optimize sample concentration prior to initial screens
  • Provide insight to sample homogeneity

Description

The PCT Pre-Crystallization Test is used to determine the appropriate protein concentration for crystallization screening. Sample concentration is a significant crystallization variable. Samples too concentrated can result in amorphous precipitate, while samples too dilute can result in clear drops. Precipitate and clear drops are typical crystallization screen results for reagent conditions which do not promote crystallization and are part of every crystallization screen. However, by optimizing protein concentration for the screen, the number of clear and precipitate results can often be reduced, which in turn results in more efficient sample utilization while at the same time enhancing the chances for crystallization. PCT can minimize or prevent situations where a screen results in an over abundance of precipitate or clear drops.

The PCT kit contains 4 unique, preformulated, sterile filtered reagents used to evaluate protein concentration for crystallization screening. Initially, the sample protein is mixed with two of the reagents to determine if the protein concentration is appropriate for crystallization screening. If the protein is very sensitive to salt and polymer concentration, based on initial PCT results, the protein may be evaluated using a second set of PCT reagents. PCT results will then provide insight to either the appropriate sample concentration or indicate that other diagnostic testing such as native gel electrophoresis or dynamic light scattering should be performed to demonstrate sample homogeneity appropriate for crystallization.

PCT Kit comparison table.

Catalog
Number
4 Unique
Reagents
Glass Siliconized
Cover Slides
24 Well Plate
with sealant
HR2-140          (50 mL each)          (22 mm Square)  
HR2-141          (10 mL each)    
HR2-142          (30 mL each)          (22 mm Square)          (5x VDX)
HR2-143          (10 mL each)          (18 mm Circle)          (4x VDXm)

 

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PCT™ Pre-Crystallization Test

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PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test

PCT™ Pre-Crystallization Test

Click to Zoom In

PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test

CAT NO

HR2-140

NAME

PCT (with slides)

DESCRIPTION

50 ml bottles (4 ea), cover slides (1 pk)

PRICE

$109.00

cart quote

CAT NO

HR2-141

NAME

PCT (reagents only)

DESCRIPTION

10 ml tubes (4 ea)

PRICE

$77.00

cart quote

CAT NO

HR2-142

NAME

PCT (with plates and slides)

DESCRIPTION

30 ml bottles (4 ea), cover slides (1 pk), VDX Plates with sealant (5 ea)

PRICE

$132.00

Temporarily Unavailable
More Available Soon!

CAT NO

HR2-143

NAME

PCT (with plates and slides)

DESCRIPTION

10 ml tubes (4 ea), cover slides (1 pk), VDXm Plates with sealant (4 ea)

PRICE

$99.00

cart quote

Support Material(s)

PCT™ Pre-Crystallization Test HR2-140 & HR2-141 & HR2-142 & HR2-143 PCT Kit DocumentsPCT™ Pre-Crystallization Test HR2-140 PCT Kit (with slides) SDSPCT™ Pre-Crystallization Test HR2-141 PCT Kit (reagents only) SDSPCT™ Pre-Crystallization Test HR2-142 PCT Kit (with plates and slides) SDSPCT™ Pre-Crystallization Test HR2-143 PCT Kit (with plates and slides) SDS

Related Item(S)

  • Individual PCT Reagents

References

1. Crystallization and x-ray diffraction analysis of human CLEC-2. Aleksandra A. Watson and Christopher A. O’Callaghan. Acta Cryst. (2005). F61, 1094-1096.

PCT™ Pre-Crystallization Test PCT™ Pre-Crystallization Test

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/8×1/4(英寸))日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/8×1/4(英寸))
产品编号: WEB10823 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )3/8×1/4(英寸)

规格(外径×内径×厚度):9.53×  6.35×1.59

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/8×1/4(英寸))NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/8×1/4(英寸))产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1/4×11/64(英寸))日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1/4×11/64(英寸))
产品编号: WEB10822 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )1/4×11/64(英寸)

规格(外径×内径×厚度):6.35×  4.35×1

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1/4×11/64(英寸))NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1/4×11/64(英寸))产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA园锥底容器(称呼:0201C、容量(ml):33ml、规格:φ29×φ25×67H)日本三博特sanplatec

PFA园锥底容器(称呼:0201C、容量(ml):33ml、规格:φ29×φ25×67H)
产品编号: WEB15265 价格: 会员价:0元;市场价:0元 产品特点
内部的园锥底可方便样品集中。
产品规格

容量(ml:33ml
整体尺寸:Φ29×Φ25×67H

附盖尺寸 (mm)外径 ×高:36Φ×70H

材料

确认材料的耐药性 >> 耐药性检索

        PFA园锥底容器(称呼:0201C、容量(ml):33ml、规格:φ29×φ25×67H)PFA园锥底容器(称呼:0201C、容量(ml):33ml、规格:φ29×φ25×67H)产品特征

特点:PFA制 、有优异耐药性同时溶出金属离子极少 ,适合保存各种分析样品。圆锥形底部更能集中样本。 耐热性,耐寒性优越,可放到冰箱内冷藏或油浴里加热,使用温度范围广,从-196度~250℃皆可使用。样品不容易粘附,更容易取出,无需特殊操作

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA园锥底容器0024(容量:5ml、规格:φ22×φ17×32H)日本三博特sanplatec

PFA园锥底容器0024(容量:5ml、规格:φ22×φ17×32H)
产品编号: WEB15264 价格: 会员价:0元;市场价:0元 产品特点
内部的园锥底可方便样品集中。
产品规格

容量(ml:5ml
整体尺寸:Φ22×Φ17×32H

附盖尺寸 (mm)外径 ×高:28Φ×36H

材料

确认材料的耐药性 >> 耐药性检索

        PFA园锥底容器0024(容量:5ml、规格:φ22×φ17×32H)PFA园锥底容器0024(容量:5ml、规格:φ22×φ17×32H)产品特征

特点:PFA制 、有优异耐药性同时溶出金属离子极少 ,适合保存各种分析样品。圆锥形底部更能集中样本。 耐热性,耐寒性优越,可放到冰箱内冷藏或油浴里加热,使用温度范围广,从-196度~250℃皆可使用。样品不容易粘附,更容易取出,无需特殊操作

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PEG/Ion • PEG/Ion 2 • PEG/Ion HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Products > Crystallization Screens > PEG/Ion • PEG/Ion 2 > PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Applications

  • Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules

Features

  • Developed at Hampton Research
  • PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 – 9.2
  • PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 – 8.8) in the presence of monodisperse PEG 3,350
  • PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block

Description

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules.

The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.

PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.

PEG/Ion contains 48 unique reagents, 10 ml each.

PEG/Ion 2 contains 48 unique reagents, 10 ml each.

PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

CAT NO

HR2-126

NAME

PEG/Ion Screen

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-098

NAME

PEG/Ion 2 Screen

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-139

NAME

PEG/Ion HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

PEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-126 PEG/Ion DocumentsPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-126 PEG/Ion SDSPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-098 PEG/Ion 2 DocumentsPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-098 PEG/Ion 2 SDSPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-139 PEG/Ion HT DocumentsPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-139 PEG/Ion HT SDSPEG/Ion • PEG/Ion 2 • PEG/Ion HT PEG/Ion Formulation & Scoring Data

Related Item(S)

  • Individual PEG/Ion • PEG/Ion 2 • PEG/Ion HT Reagents

References

1. Expression, purification, crystallization and preliminary X-ray diffraction analysis of Bifidobacterium adolescentis xylose isomerase. C. V. dos Reis, A. Bernardes and I. Polikarpov. Acta Cryst. (2013). F69, 588-591.

2. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

3. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology Volume 156, Issue 3 , December 2006, Pages 387-406.

4. Mechanisms for Glycolipid Antigen-Driven Cytokine Polarization by Valpha14i NKT Cells. Sulliivan et al, The Journal of Immunology, 2010, 184, 141 -153. (PEG/Ion 2)

5. Optimization of salt concentration in PEG-based crystallization solutions. Yamanaka M, Inaka K, Furubayashi N, Matsushima M, Takahashi S, Tanaka H, Sano S, Sato M, Kobayashi T, Tanaka T. J Synchrotron Radiat. 2011 Jan;18(1):84-7. doi: 10.1107/S0909049510035995. Epub 2010 Nov 5.

6. Crystallization and preliminary X-ray crystallographic analysis of importin-[alpha] from Neurospora crassa. N. E. Bernardes, A. A. S. Takeda, F. Z. Freitas, M. C. Bertolini and M. R. M. Fontes. Acta Cryst. (2014). F70, 501-504 [ doi:10.1107/S2053230X14005068 ]

PEG/Ion • PEG/Ion 2 • PEG/Ion HT PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 19×16)日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 19×16)
产品编号: WEB10820 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )19×16

规格(外径×内径×厚度):19×16×1.5

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 19×16)NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 19×16)产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

180ml PFA样品容器日本三博特sanplatec

180ml PFA样品容器
产品编号: WEB11961 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格
/
材料

确认材料的耐药性 >> 耐药性检索

        180ml PFA样品容器180ml PFA样品容器产品特征

特点:无金属。 适用于水质检测、 ICP分析等实验。 与 PFA平低 /圆锥底容器相比透明度更高、 价格更便宜。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 14×12)日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 14×12)
产品编号: WEB10819 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )14×12

规格(外径×内径×厚度):14×12×1

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 14×12)NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 14×12)产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

90ml PFA样品容器日本三博特sanplatec

90ml PFA样品容器
产品编号: WEB11960 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格
/
材料

确认材料的耐药性 >> 耐药性检索

        90ml PFA样品容器90ml PFA样品容器产品特征

特点:无金属。 适用于水质检测、 ICP分析等实验。 与 PFA平低 /圆锥底容器相比透明度更高、 价格更便宜。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 12×10)日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 12×10)
产品编号: WEB10818 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )12×10

规格(外径×内径×厚度):12×10×1

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 12×10)NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 12×10)产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

60ml PFA样品容器日本三博特sanplatec

60ml PFA样品容器
产品编号: WEB11959 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格
/
材料

确认材料的耐药性 >> 耐药性检索

        60ml PFA样品容器60ml PFA样品容器产品特征

特点:无金属。 适用于水质检测、 ICP分析等实验。 与 PFA平低 /圆锥底容器相比透明度更高、 价格更便宜。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PEG/Ion 400蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

PEG/Ion 400
PEG/Ion 400

PEG/Ion 400

PEG/Ion 400

PEG/Ion 400

PEG/Ion 400

Products > Crystallization Screens > PEG/Ion 400 > PEG/Ion 400

PEG/Ion 400

Applications

  • Crystallization screen for membrane and soluble biological macromolecules.

Features

  • Reagent formulation developed at Hampton Research
  • Screens a profile of anions, cations, multivalent ions, and neutralized organic acids at varying pH levels in the presence of Polyethylene glycol 400
  • pH range 5 – 9
  • Buffer free formulation
  • Compatible with lipidic cubic phase (LCP), vapor diffusion, microbatch, and free interface diffusion

Description

PEG/Ion 400 is a crystallization reagent kit designed to provide a rapid screening method for the crystallization of membrane and soluble biological macromolecules.

Most G protein-coupled receptors (GPCRs) have been crystallized in Polyethylene glycol (PEG) 400 based reagents1. PEG/Ion 400 is designed as a 96 reagent crystallization screen that is compatible with the Lipidic Cubic Phase (LCP).

PEG/Ion 400 is supplied in a sterile, polypropylene 96 Deep Well block, each reservoir containing 1 ml of sterile filtered reagent. The block is compatible with robotic and multi-channel pipet liquid handling systems and is heat sealed using a special polypropylene backed film. Each PEG/Ion 400 is supplied with an adhesive sealing film which can be used to seal the block after removing the heat seal.

The screen combines a single concentration (30% v/v) of high purity Polyethylene glycol 400 and 48 different high purity salts, comprising both anions (malate, malonate, nitrate, phosphate, succinate, sulfate, tartrate, and thiocyanate) and cations (ammonium, cadmium, calcium, ethylammonium, lithium, magnesium, nickel, potassium, sodium, tetraethylammonium, and zinc) in two concentrations (0.1 and 0.4 M) which due to their unique pH characteristics also affords a reasonable pH screen (approximate pH range of 4 to 9.5). The primary screen variables are PEG, ion type, ionic strength, salt concentration, and pH.

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PEG/Ion 400

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PEG/Ion 400
PEG/Ion 400
PEG/Ion 400

PEG/Ion 400

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PEG/Ion 400
PEG/Ion 400
PEG/Ion 400

CAT NO

HR2-460

NAME

PEG/Ion 400

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

PEG/Ion 400 HR2-460 PEG/Ion 400 DocumentsPEG/Ion 400 HR2-460 PEG/Ion 400 SDSPEG/Ion 400 PEG/Ion 400 Formulation & Scoring Data

Related Item(S)

  • Individual PEG/Ion 400 Reagents

References

1. Successful Strategies to Determine High-Resolution Structures of GPCRs. Xiang J, Chun E, Liu C, Jing L, Al-Sahouri Z, Zhu L, Liu W. Trends Pharmacol Sci. 2016 Dec;37(12):1055-1069.

2. Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. Kang Y, Zhou XE, Gao X, et al. Nature. 2015;523(7562):561-567. doi:10.1038/nature14656.

3. Development of an Automated High Throughput LCP-FRAP Assay to Guide Membrane Protein Crystallization in Lipid Mesophases. Fei Xu, Wei Liu, Michael A. Hanson, Raymond C. Stevens, and Vadim Cherezov. Cryst. Growth Des., 2011, 11 (4), pp 1193-1201.

4. A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes. Martin Caffrey. Acta Crystallogr F Struct Biol Commun. 2015 Jan 1; 71(Pt 1): 3-18.

PEG/Ion 400 PEG/Ion 400

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 10×8)日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 10×8)
产品编号: WEB10817 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )10×8

规格(外径×内径×厚度):10×  8×1

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 10×8)NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 10×8)产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 8×6)日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 8×6)
产品编号: WEB10816 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )8×6

规格(外径×内径×厚度):8×  6×1

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 8×6)NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 8×6)产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

pfa平底容器0025 (容量15ml)日本三博特sanplatec

pfa平底容器0025 (容量15ml)
产品编号: WEB15275 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

整体尺寸(mm)(外径×内径×高):29×  25×  36

瓶盖尺寸(mm)(外径×高):36×  40

材料

确认材料的耐药性 >> 耐药性检索

        pfa平底容器0025 (容量15ml)pfa平底容器0025 (容量15ml)产品特征

使用温度范围:-196~200℃

最适合用于碱分解、硅烷化等不能使用玻璃的反应。

注射成形,有质感,非常坚固。

特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA小沉淀管(附11mm推盖,容量:1.5ml、径×高(mm):19.3φ×42.4)日本三博特sanplatec

PFA小沉淀管(附11mm推盖,容量:1.5ml、径×高(mm):19.3φ×42.4)
产品编号: WEB23034 价格: 会员价:0元;市场价:0元 产品特点
防止样品污染。
产品规格
材料

确认材料的耐药性 >> 耐药性检索

        PFA小沉淀管(附11mm推盖,容量:1.5ml、径×高(mm):19.3φ×42.4)PFA小沉淀管(附11mm推盖,容量:1.5ml、径×高(mm):19.3φ×42.4)产品特征: .  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PEGRx 1 • PEGRx 2 • PEGRx HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

Products > Crystallization Screens > PEGRx 1 • PEGRx 2 > PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

Applications

  • Primary and secondary, polymer and pH based crystallization screen for biological macromolecules

Features

  • Developed at Hampton Research
  • PEGRx 1 primary screen variables are polymer type (16 different polymers), polymer molecular weight, pH and low ionic strength.
  • PEGRx 2 primary screen variables are polymer type (13 different polymers), polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.
  • PEGRx HT combines PEGRx 1 and PEGRx 2 into a single 96 Deep Well block
  • pH range 3.5 – 9, using 10 different buffer systems
  • Polymer molecular weight range 200 to 20,000
  • Ligand friendly, complex friendly

Description

PEGRx is a primary and secondary, polymer and pH based crystallization screen developed at Hampton Research.

It is designed to evaluate polymer based crystallization reagents and pH in low (PEGRx 1) to medium ionic strength (PEGRx 2). It is also designed for use as a secondary or optimization screen to follow the Hampton Research Index screen when polymer based reagents produce hits and interesting solubility leads.

PEGRx 1 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a low ionic strength environment versus a wide range of pH. Polymer reagents include Polyethylene glycols, Polyethylene glycol monomethyl ethers, and Jeffamines®. The molecular weight range between 200 and 20,000 is evaluated in a low ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and low ionic strength.

PEGRx 2 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a medium ionic strength environment in the presence of additives, salts, volatile organics and polyols versus a wide range of pH. Polymer reagents include Polyethylene glycols and Polyethylene glycol monomethyl ethers. The polymer molecular weight range between 200 and 20,000 is evaluated in a medium ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.

PEGRx 1 contains 48 unique reagents, 10 ml each.

PEGRx 2 contains 48 unique reagents, 10 ml each.

PEGRx HT contains the 96 PEGRx 1 and PEGRx 2 reagent in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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PEGRx 1 • PEGRx 2 • PEGRx HT

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PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx 1 • PEGRx 2 • PEGRx HT

Click to Zoom In

PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT

CAT NO

HR2-082

NAME

PEGRx 1

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-084

NAME

PEGRx 2

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-086

NAME

PEGRx HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

PEGRx 1 • PEGRx 2 • PEGRx HT HR2-082 PEGRx 1 DocumentsPEGRx 1 • PEGRx 2 • PEGRx HT HR2-082 PEGRx 1 SDSPEGRx 1 • PEGRx 2 • PEGRx HT HR2-084 PEGRx 2 DocumentsPEGRx 1 • PEGRx 2 • PEGRx HT HR2-084 PEGRx 2 SDSPEGRx 1 • PEGRx 2 • PEGRx HT HR2-086 PEGRx HT DocumentsPEGRx 1 • PEGRx 2 • PEGRx HT HR2-086 PEGRx HT SDSPEGRx 1 • PEGRx 2 • PEGRx HT Change in Formulation PEGRx ScreensPEGRx 1 • PEGRx 2 • PEGRx HT PEGRx Formulation & Scoring Data

Related Item(S)

  • Individual PEGRx 1 • PEGRx 2 • PEGRx HT Reagents

References

1. Crystallization and preliminary X-ray crystallographic studies of DesR, a thermosensing response regulator in a two-component signalling system from Streptococcus pneumoniae. Ae Kyung Park, Seung Min Bong, Jin Ho Moon and Young Min Chi. Acta Cryst. (2009). F65.

2. Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors.  Zhang L, Lin D, Sun X, Curth U, Drosten C, Sauerhering L, Becker S, Rox K, Hilgenfeld R. Science. 2020 Apr 24;368(6489):409-412. doi: 10.1126/science.abb3405. Epub 2020 Mar 20.

3. Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase.  Zhang, L., Xie, Z., Liu, Z. et al.  Nat Commun 11, 2676 (2020). https://doi.org/10.1038/s41467-020-16500-5.

PEGRx 1 • PEGRx 2 • PEGRx HT PEGRx 1 • PEGRx 2 • PEGRx HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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  • Products
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© 2022 HAMPTON RESEARCH CORP.


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NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 6×4)日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 6×4)
产品编号: WEB10815 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )6×4

规格(外径×内径×厚度):6×  4×1

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 6×4)NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 6×4)产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA小沉淀管(容量:1.5ml,径×高(mm):12.7φ×40.1)日本三博特sanplatec

PFA小沉淀管(容量:1.5ml,径×高(mm):12.7φ×40.1)
产品编号: WEB23033 价格: 会员价:0元;市场价:0元 产品特点
防止样品污染。
产品规格
材料

确认材料的耐药性 >> 耐药性检索

        PFA小沉淀管(容量:1.5ml,径×高(mm):12.7φ×40.1)PFA小沉淀管(容量:1.5ml,径×高(mm):12.7φ×40.1)产品特征: .  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):14φ×12φ×1.0)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):14φ×12φ×1.0)
产品编号: WEB5690 价格: 会员价:0元;市场价:0元 产品特点
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产品规格
可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):14φ×12φ×1.0)
材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):14φ×12φ×1.0)可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):14φ×12φ×1.0)产品特征

使用有耐热性,低摩擦性,电绝缘性,耐药品性,非粘性,耐候性等许多出色特长的 PFA。在电子学领域,生 物技术领域,医疗,食品领域,精细化工等的各种领域中广泛使用。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):12φ×10φ×1.0)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):12φ×10φ×1.0)
产品编号: WEB5688 价格: 会员价:0元;市场价:0元 产品特点
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产品规格
可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):12φ×10φ×1.0)
材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):12φ×10φ×1.0)可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):12φ×10φ×1.0)产品特征

使用有耐热性,低摩擦性,电绝缘性,耐药品性,非粘性,耐候性等许多出色特长的 PFA。在电子学领域,生 物技术领域,医疗,食品领域,精细化工等的各种领域中广泛使用。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA小沉淀管(附8mm推盖,容量:0.5ml、径×高(mm):15.7φ×30.7)日本三博特sanplatec

PFA小沉淀管(附8mm推盖,容量:0.5ml、径×高(mm):15.7φ×30.7)
产品编号: WEB23032 价格: 会员价:0元;市场价:0元 产品特点
防止样品污染。
产品规格
材料

确认材料的耐药性 >> 耐药性检索

        PFA小沉淀管(附8mm推盖,容量:0.5ml、径×高(mm):15.7φ×30.7)PFA小沉淀管(附8mm推盖,容量:0.5ml、径×高(mm):15.7φ×30.7)产品特征: .  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):10φ×8φ×1.0)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):10φ×8φ×1.0)
产品编号: WEB5686 价格: 会员价:0元;市场价:0元 产品特点
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产品规格
可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):10φ×8φ×1.0)
材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):10φ×8φ×1.0)可熔性聚四氟乙烯(PFA)管(10m)(外径×内径×厚度(mm):10φ×8φ×1.0)产品特征

使用有耐热性,低摩擦性,电绝缘性,耐药品性,非粘性,耐候性等许多出色特长的 PFA。在电子学领域,生 物技术领域,医疗,食品领域,精细化工等的各种领域中广泛使用。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

Proti-Ace • Proti-Ace 2蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Proti-Ace • Proti-Ace 2
Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Products > Optimization Screens > Proti-Ace > Proti-Ace • Proti-Ace 2

Proti-Ace • Proti-Ace 2

Applications

  • Limited proteolysis, in situ proteolysis, and proteolytic screening of protein samples for crystallization and structure determination

Features

  • Proti-Ace proteases
    • alpha-Chymotrypsin
    • Trypsin
    • Elastase
    • Papain
    • Subtilisin
    • Endoproteinase Glu-C
  • Proti-Ace 2 proteases
    • Proteinase K
    • Clostripain (Endoproteinase-Arg-C)
    • Pepsin
    • Thermolysin
    • Bromelain
    • Actinase E
  • Optimized, stable, freeze dried protease formulation

Description

A proteolytic fragment or domain of a protein may crystallize more readily or form better diffracting crystals than the intact protein.1-8

Proteases can be used to generate small, active fragments or domains of the target protein for crystallization.9 The fragment or domain can be used directly for crystallization experiments. Or the proteolytic sample analyzed by gel electrophoresis and/or mass spectrometry for mass and sequence for subsequent cloning, expression, purification and crystallization. Using proteolysis to enhance sample crystallization, the current overall success rate for yielding a deposited crystal structure is currently better than 12%.3

Proti-Ace (HR2-429) contains three aliquots of 6 unique proteases (a-Chymotrypsin, Trypsin, Elastase, Papain, Subtilisin and Endoproteinase Glu-C) and six aliquots of Proti-Ace Dilution Buffer. Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.

Proti-Ace 2 (HR2-432) contains three aliquots of 6 unique proteases (Proteinase K, Clostripain (Endoproteinase-Arg-C), Pepsin, Thermolysin, Bromelain and Actinase E) and six aliquots of Proti-Ace Dilution Buffer. Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.

The unique freeze dried formulation of the Proti-Ace kits offers a much improved protease stability compared to liquid protease formulations.

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Proti-Ace • Proti-Ace 2
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Proti-Ace • Proti-Ace 2

CAT NO

HR2-429

NAME

Proti-Ace™

DESCRIPTION

tube format

PRICE

$274.00

cart quote

CAT NO

HR2-432

NAME

Proti-Ace™ 2

DESCRIPTION

tube format

PRICE

$274.00

cart quote

Support Material(s)

Proti-Ace • Proti-Ace 2 HR2-429 Proti-Ace User GuideProti-Ace • Proti-Ace 2 HR2-429 Proti-Ace SDSProti-Ace • Proti-Ace 2 HR2-432 Proti-Ace 2 User GuideProti-Ace • Proti-Ace 2 HR2-432 Proti-Ace 2 SDSProti-Ace • Proti-Ace 2 Proti-Ace Enzyme Mr pI Specificty Source

Related Item(S)

  • Individual Proti-Ace & Proti-Ace 2 Reagents

References

1. Allan D′Arcy, personal communication, 1989-2009.

2. In situ proteolysis for protein crystallization and structure determination. Dong, A et al. Nature Methods – 4, 1019 – 1021 (2007).

3. In Situ Proteolysis to Generate Crystals for Structure Determination: An Update. Amy Wernimont, Aled Edwards. PLoS ONE 4(4): e5094. doi:10.1371/ journal.pone.0005094.

4. The use of in situ proteolysis in the crystallization of murine CstF-77. Tong et al. Acta Cryst. (2007). F63, 135-138.

5. A brief history of protein crystal growth. McPherson, A. Journal of Crystal Growth, vol. 110, issue 1-2, pp. 1-10, 1991.

6. Preparation and analysis of protein crystals. McPherson, A. John Wiley & Sons, Inc. 1982. ISBN 089464355X.

7. A crystallizable form of the Streptococcus gordonii surface antigen SspB C-domain obtained by limited proteolysis. Forsgren et al. Acta Cryst. (2009). F65, 712-714.

8. Preliminary X-ray analysis of a human VH fragment at 1.8 angstrom resolution. Gaur, Kupper, Fischer & Hoffman. Acta Cryst. (2004). D60, 965-967.

9. Replication Protein A Characterization and Crystallization of the DNA Binding Domain. Pfuetzner et al. The Journal of Biological Chemistry, Vol. 272, No. 1, Issue of January 3, pp. 430-434, 1997.

10. Combining in situ proteolysis and mass spectrometry to crystallize Escherichia coli PgaB. Little et al. Acta Cryst. (2012) F68, 842-845.

11. Proteolysis of Native Proteins – Trapping of a Reaction Intermediate. Chenyi Wu, Duncan H. L. Robertson, Simon J. Hubbard, Simon J. Gaskell and Robert J. Beynon. doi: 10.1074/jbc.274.2.1108 January 8, 1999 The Journal of Biological Chemistry, 274, 1108-1115.

12. Crystallization of mouse RIG-I ATPase domain: in situ proteolysis. Civril F, Hopfner KP. Methods Mol Biol. 2014;1169:27-35. doi: 10.1007/978-1-4939-0882-0_3.

13. Crystallization and preliminary X-ray crystallographic analysis of YfcM: an important factor for EF-P hydroxylation. K. Kobayashi, T. Suzuki, N. Dohmae, R. Ishitani and O. Nureki. Acta Cryst. (2014). F70, 1236-1239 [ doi:10.1107/S2053230X14015726 ]. Synopsis: in situ proteolysis crystallization method produces crystals diffracting to 1.45 Å

Proti-Ace • Proti-Ace 2 Proti-Ace • Proti-Ace 2

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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