4-硝基苯基-α-D-木糖 4-Nitrophenyl-α-D-xylopyranoside 货号:O-PNPAX Megazyme中文站

4-硝基苯基-α-D-木糖

英文名:4-Nitrophenyl-α-D-xylopyranoside

货号:O-PNPAX

规格:100 mg

Synonyms: p-Nitrophenyl-α-D-xylopyranoside, 
                   pNP-α-D-xylopyranoside
CAS: 10238-28-5
Molecular Formula: C11H13NO
7
Molecular Weight: 271.2
Purity: > 98%

High purity 4-Nitrophenyl-α-D-xylopyranoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for measurement of α-xylosidase activity.

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牛皮源去端肽胶原


产品编号 产品名称 产品规格 产品等级 产品价格
KOU-IPC-30 Atelocollagen, Bovine dermis, 3mg/mL 
去端肽胶原,牛皮来源,3 mg/mL
50ml/btl
KOU-IPC-50 Atelocollagen Bovine dermis 5mg/ml pH3.0 Steril 
去端肽胶原,牛皮来源,5 mg/mL
50ml/btl
KOU-IAC-30 Native collagen Bovine dermis 3mg/ml pH3.0 Steril 
天然胶原,牛皮来源,3 mg/mL
50ml/btl
KOU-IAC-50 Native collagen Bovine dermis 5mg/ml pH3.0 Steril 
天然胶原,牛皮来源,5 mg/mL
50ml/btl

  AteloCell™ Atelocollagen,Native collagen牛皮源去端肽胶原

◆原理

Cosmo Bio公司以先进的产品技术,优良的质量监控为广大客户提供用于细胞培养的胶原溶液。

Atelocollagen (端胶原)(IPC):胃蛋白溶解I型胶原溶液

Native collagen(天然胶原)(IAC):酸溶解I型胶原溶液

◆优点特色


所有产品均已灭菌。胶原蛋白从牛的真皮中提取,经过新颖的方法进行溶解纯化。在37℃,端胶原与天然胶原会形成纤维。

 

◆案例应用


胶原包被在培养皿上;细胞在胶原上三维培养



35mm胶原海绵培养皿

◆原理

胶原海绵细胞培养是以胶原蛋白为基础新开发出来的,可用于细胞3D培养。多孔胶原海绵是由牛跟腱提取的不溶性I型胶原蛋白制备而成。细胞可渗透入海绵,朝不同方向增殖。

◆优点特色

       所有产品均已灭菌 

◆案例应用

细胞在海绵上培养


产品编号

品名

包装

储存

KOU-CS-35

Collagen sponge for35mm culture dish Bovine tendon   Sterile

5pcs/box

RT

 


Atelocollagen, Permeable membrane for50mmculture dish

◆原理

从牛跟腱中提取的I型胶原蛋白制备的可渗透性胶原蛋白膜可用于单层或双层细胞培养。此胶原蛋白膜特别适用于研究在膜两侧培养的不同细胞间的分子相互作用,相互关系。膜的可渗透性允许氨基酸,蛋白质等小分子自由通过胶原蛋白膜,这对于细胞间的营养吸收,物质交换有重要作用。

◆优点特色


 两种不同细胞可在膜两侧生长,因此特别适合研究细胞或小分子相互间的作用。

 可渗透性膜非常适合细胞培养,也适合人工皮肤,人工胰腺等其它人工器官的培养。

产品编号

品名

规格

储存

KOU-MEN-01

Atelocollagen, Permeable membrane for50mmculture   dish

(33mm)Bovine   dermis   Sterile

5pcs/box

-20℃

 


 

Atelocollagen, Honeycomb sponge

◆原理

“蜂窝状”海绵的结构比较特殊,它的孔径大约200-400μm,密集地向一个方向排列,因此细胞可以顺着小孔渗透增殖。这样的结构,有利于海绵内部的细胞吸收营养物质,排出生物代谢物质。细胞在空腔内增殖,并形成统一的细胞团。

优势:从牛跟腱中提取胶原蛋白制备“蜂窝状”海绵,可以用胶原酶降解此海绵。

 牛皮源去端肽胶原牛皮源去端肽胶原Honeycomb sponge的电镜图

 

产品编号

品名

规格

储存

KOU-CSH-10

Atelocollagen Honeycomb sponge Bovine dermis

Size: about 3X3X2 mm/sponge   total surface area:2000cm2/g  weight per volume:   12-13mg/ml sterile

100mg/btl

室温


牛皮源去端肽胶原

AteloCell 细胞培养胶原.pdf

氨基酸混合标准溶液, B型


产品编号 产品名称 产品规格 产品等级 产品价格
012-08643 Amino Acids Mixture Standard Solution, Type B
氨基酸混合标准溶液, B型
1mLx5A for Amino Acid Automated Analysis
016-08641 Amino Acids Mixture Standard Solution, Type B
氨基酸混合标准溶液, B型
5mL for Amino Acid Automated Analysis

氨基酸混合标准溶液,B型   Amino Acids Mixture Standard Solution, Type B

 

  Wako生产的氨基酸混合标准可用于氨基酸自动分析仪或高效液相色谱(HPLC)进行氨基酸成分分析或测序。

组成成分:(每种氨基酸浓度2.50μmol/ml)


γ-氨基丁酸; 

乙醇胺; 

氯化铵; 

异羟赖氨酸;

L-鸟氨酸;

L-赖氨酸;

1-甲基-L-组氨酸;

L-组氨酸;

L-3-甲基组氨酸; 

L-鹅肌肽; 

L-肌肽;

L-精氨酸

 

相关产品

 

产品编号

产品名称

包装

011-14463 

Amino Acids Mixture Standard Solution, Type AN-2

1mL×5A

015-14461 

5mL

013-08391

Amino Acids Mixture Standard Solution, Type H

5mL

019-08393

1mL x5

六乙酰壳六糖 Hexaacetyl-Chitohexaose- 10mg 货号:O-CHI6 Megazyme中文站

六乙酰壳六糖

英文名:Hexaacetyl-Chitohexaose- 10mg

货号:O-CHI6

规格:10 mg

CAS: 38854-46-5
Molecular Formula: C48H80N6O31
Molecular Weight: 1237.2
Purity: > 90%

High purity Hexaacetyl-chitohexaose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Prepared from chitin.

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merck millipore,默克密理博 AG310-30MG | FLUORO-JADE® B

AG310-30MG | FLUORO-JADE® B

merck millipore,默克密理博 AG310-30MG | FLUORO-JADE® B

Description
Catalogue Number AG310-30MG
Trade Name
  • Fluoro-Jade
Description FLUORO-JADE® B
Overview N-terminal GST-tagged, recombinant human XIAP full length, expressed by baculovirus in Sf21 insect cells
Background Information Fluoro-JadeⓇ B is a polyanionic fluorescein derivative which sensitively and specifically binds to degenerating neurons. It is a dark red power that has a green iridescence with excitation peak at 480 nm and emission peak at 525 nm. The filter used for visualizing Fluoro-JadeⓇ B is a fluorescein/FITC filter. Fluoro-JadeⓇ B can be used on most tissue section types and thicknesses including frozen, vibratomed cryostat or paraffin-embedded sections from 3-50 µm. Fluoro-JadeⓇ B is faster and more reliable than older methods (e.g. suppressed silver) for the unequivocal qualitative detection and quantitative measurement of both and fine scale neuronal degeneration.

Product Information
Formulation
Presentation Solubility: Very soluble in water and bases, moderately soluble in alcohol and weak acids.

Applications
Key Applications
  • Immunofluorescence
Application Notes Appearance: Dark red powder with green iridescence.

Biological Information
Purity This layer chromatography using cellulose plates and a solvent system of n-propinol, water, and ammonium hydroxide (6:5:2) revealed the presence of two fluorescent isomers and two trace non-fluorescent bands. No amount of fluorescein or Fluoro-Jade was present
Molecular Weight 681

Physicochemical Information
Emission max.
  • 525 nm
Excitation max.
  • 480 nm
Sensitivity
  • Toxicity: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.

Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions The powder should be stored well sealed at room temperature, preferable in a dessicator, due to its hygroscopic nature. The liquid stock solution (0.01%) in distilled water can be stored at 2-8°C for up to 3 months. The working solution (0.0004%) in 0.1% acetic acid should be prepared fresh and not be stored or reused.

Packaging Information
Material Size 30 mg

α-(1-2,3,4,6)-D-甘露糖苷酶(海栖热袍) α-(1-2,3,4,6)-D-Mannosidase (Thermotoga maritima) 货号:E-AMANTM Megazyme中文站

α-(1-2,3,4,6)-D-甘露糖苷酶(海栖热袍)

英文名:α-(1-2,3,4,6)-D-Mannosidase (Thermotoga maritima)

货号:E-AMANTM

规格:5 Units at 90°C

High purity α-Mannosidase (Thermotoga maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.24
CAZy Family: GH38
CAS: 9025-42-7

alpha-mannosidase; alpha-D-Mannoside mannohydrolase, alpha-D-mannopyranosidase

Recombinant. From Thermotoga maritima.
In 3.2 M ammonium sulphate.
Supplied at ~ 71 U/mL. 

Specific activity: 4.5 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.0 and 90oC; ~ 0.2 U/mg at 40oC.

Stability: > 4 years 4oC.

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总亚硫酸盐检测试剂盒(酶法) Total Sulfite Assay Kit (Enzymatic) 货号:K-ETSULPH Megazyme中文站

总亚硫酸盐检测试剂盒(酶法)

英文名:Total Sulfite Assay Kit (Enzymatic)

货号:K-ETSULPH

规格:50 assays (manual) / 500 assays (microplate)

The Total Sulfite (Enzymatic) test kit is a simple, highly specific and reliable, enzymatic method for the measurement and analysis of total sulfite (as SO2) in wines, beverages, foodstuffs and other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Total Sulfite (SO22-) in
beverages,foodstuffs and other materials

Principle:
(sulfite oxidase)
(1) SO32- + O2 + H2O ↔ SO42- + H2O2

(NADH – peroxidase)
(2) H2O2 + NADH + H+ → 2 H2O + NAD+

Kit size: 50 assays (manual) / 500 (microplate)
/ 588 (auto-analyser)
Method: Spectrophotometric at 340 nm
Total assay time: ~ 30 min
Detection limit: 0.3 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, jam, fruit and vegetables,
pharmaceuticals and other materials
Method recognition:
Methods based on this principle have been accepted by DIN, EN,
MEBAK and NMKL

Advantages

  • Very cost effective
  • All reagents stable for > 2 years during use
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

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HyClone培养基目录

HyClone培养基目录
HyClone公司创立于1967年,总部位于美国犹他州洛根市,是细胞培养和相关产品的全球供应商。现在从属于世界著名的FISHER科学国际公司。
近40年来,HyClong公司一直是高品质胎牛血清(FBS)及其他血清产品行业的领导者,各种血清产品多年来稳居美国市场占有率第一名。自2000年起美国模式培养物保藏所(ATCC)对所有HyClone的血清实行免检采购。
HyClone还大批量生产各类经典的细胞培养基、不断推陈出新的无血清和无蛋白培养基,并且可以根据客户的批量型配方要求定制培养基。
HyClone液体培养基在使用的过程中要注意以下问题:
运输中要避光冷藏,瓶口保持向上,切勿剧烈振荡;
开瓶后不要长时间暴露在空气和强光下,以保持培养基的稳定性;
开瓶后zui好能在一周内用完,短时间内用不完可分装冷藏保存,但PH值会在随后的时间里慢慢变碱,属于正常现象,请放心使用;
所有液体培养基均经100纳米无菌过滤和出厂严格检测,除非需要,请不要私自进行二次过滤和任何形式的无菌检测。

普通液体培养基列表
描述 货号 包装 储存温度
BME培养基(Basal Medium Eagle)
EBSS*,含L-谷氨酰胺。 ● SH30157.01 500 ml 2-8°C
○ SH30157.02 1000 ml 2-8°C
DMEM培养基(Dulbecco’s Modified Eagle’s Medium)
高糖,含4.0 mM L-谷氨酰胺,不含丙酮酸钠。 ● SH30022.01B 500 ml 2-8°C
● SH30022.FS 6 x 500 ml 2-8°C
● SH30022.02B 1000 ml 2-8°C
● SH30022.LS 6 x 1000 ml 2-8°C
高糖,不含L-谷氨酰胺与丙酮酸钠。 ● SH30081.01 500 ml 2-8°C
● SH30081.FS 6 x 500 ml 2-8°C
● SH30081.02 1000 ml 2-8°C
● SH30081.LS 6 x 1000 ml 2-8°C
○ SH30081.03 20 L袋装 2-8°C
○ SH30081.04 10 L袋装 2-8°C
高糖,含4.0 mM L-谷氨酰胺、丙酮酸钠。 ●SH30243.01B 500 ml 2-8°C
●SH30243.FS 6 x 500 ml 2-8°C
●SH30243.02 1000 ml 2-8°C
●SH30243.LS 6 x 1000 ml 2-8°C
○SH30243.03 10 L袋装 2-8°C
○SH30243.04 20 L袋装 2-8°C
高糖,含L-谷氨酰胺、HEPES,不含丙酮酸钠。 ●SH30249.01 500 ml 2-8°C
●SH30249.02 1000 ml 2-8°C
高糖,不含钙、镁。 ●SH30262.01 500 ml 2-8°C
○SH30262.02 1000 ml 2-8°C
高糖,含L-谷氨酰胺,不含酚红与丙酮酸钠。 ●SH30284.01 500 ml 2-8°C
●SH30284.02 1000 ml 2-8°C
高糖,含丙酮酸钠,不含L-谷氨酰胺。 ●SH30285.01 500 ml 2-8°C
●SH30285.FS 6 x 500 ml 2-8°C
●SH30285.02 1000 ml 2-8°C
●SH30285.LS 6 x 1000 ml 2-8°C
○SH30285.03 20 L袋装 2-8°C
○SH30285.04 10 L袋装 2-8°C
高糖,改良型,不含酚红、丙酮酸钠、L-谷氨酰胺。 ○SH30585.01 500 ml 2-8°C
●SH30585.02 1000 ml 2-8°C
高糖,改良型,不含酚红、L-谷氨酰胺,含丙酮酸钠。 ●SH30604.01 500 ml 2-8°C
●SH30604.02 1000 ml 2-8°C
高糖,含丙酮酸钠,不含L-谷氨酰胺、甲硫氨酸、胱氨酸。 ○SH30606.01 500 ml 2-8°C
○SH30606.02 1000 ml 2-8°C
高度改良,含丙酮酸钠、25 mM HEPES,不含L-谷氨酰胺。 ○SH30563.01 500 ml 2-8°C
○SH30563.02 1000 ml 2-8°C
高糖,含丙酮酸钠,不含L-谷氨酰胺、磷酸盐。 ○SH30607.01 500 ml 2-8°C
低糖,含4.0 mM L-谷氨酰胺、110 mg丙酮酸钠。 ●SH30021.01B 500 ml 2-8°C
●SH30021.FS 6 x 500 ml 2-8°C
●SH30021.02 1000 ml 2-8°C
含2.50 mM L-谷氨酰胺、15 mM HEPES。 ●SH30023.01B 500 ml 2-8°C
●SH30023.FS 6 x 500 ml 2-8°C
●SH30023.02 1000 ml 2-8°C
含HEPES,不含L-谷氨酰胺。 ●SH30126.01 500 ml 2-8°C
●SH30126.FS 6 x 500 ml 2-8°C
○SH30126.02 1000 ml 2-8°C
含L-谷氨酰胺、HEPES、丙酮酸钠。 ●SH30261.01 500 ml 2-8°C
●SH30261.02 1000 ml 2-8°C
含L-谷氨酰胺,不含HEPES。 ●SH30271.01 500 ml 2-8°C
●SH30271.FS 6 x 500 ml 2-8°C
●SH30271.02 1000 ml 2-8°C
含L-谷氨酰胺,不含HEPES、酚红。 ●SH30272.01 500 ml 2-8°C
●SH30272.02 1000 ml 2-8°C
Ham”s培养基 (Ham’s Nutrient Mixture)
F10培养基,含L-谷氨酰胺。 ●SH30025.01 500 ml 2-8°C
○SH30025.02 1000 ml 2-8°C
F12培养基,含1.00 mM L-谷氨酰胺。, 1X ●SH30026.01B 500 ml 2-8°C
●SH30026.FS 6 x 500 ml 2-8°C
●SH30026.02 1000 ml 2-8°C
F12培养基, Kaighn”s改良型。 ●SH30526.01 500 ml 2-8°C
○SH30526.02 1000 ml 2-8°C
昆虫培养基 (Insect Media)
Grace”s无添加剂培养基 ○SH30610.01 500 ml 2-8°C
○SH30610.02 1000 ml 2-8°C
TNM-FH培养基 ●SH30280.02 500 ml 2-8°C
●SH30280.03 1000 ml 2-8°C
IMDM培养基 (Iscove”s Modified Dulbecco”s Medium)
含L-谷氨酰胺、HEPES,不含α-硫代甘油。 ●SH30228.01B 500 ml 2-8°C
●SH30228.FS 6 x 500 ml 2-8°C
●SH30228.02 1000 ml 2-8°C
不含L-谷氨酰胺。 ●SH30259.01 500 ml 2-8°C
●SH30259.02 1000 ml 2-8°C
Leibovitz培养基 (Leibovitz Medium)
L-15培养基,含2.05 mM L-谷氨酰胺。 ●SH30525.01 500 ml 2-8°C
●SH30525.02 1000 ml 2-8°C
M199培养基 (Medium 199)
EBSS*,含L-谷氨酰胺。 ●SH30253.01B 500 ml 2-8°C
○SH30253.FS 6 x 500 ml 2-8°C
●SH30253.02 1000 ml 2-8°C
HBSS*,含L-谷氨酰胺。 ○SH30233.01 100 ml 2-8°C
○SH30233.02 500 ml 2-8°C
HBSS*,含L-谷氨酰胺、L-氨基酸。 ○SH30330.01 500 ml 2-8°C
○SH30330.02 1000 ml 2-8°C
McCoy”s 5A培养基 (McCoy”s 5A Medium)
含L-谷氨酰胺。 ●SH30200.01 500 ml 2-8°C
●SH30200.FS 6 x 500 ml 2-8°C
●SH30200.02 1000 ml 2-8°C
含L-谷氨酰胺,不含酚红。 ●SH30270.01 500 ml 2-8°C
○SH30270.02 1000 ml 2-8°C
含L-谷氨酰胺、HEPES。 ○SH30602.01 500 ml 2-8°C
○SH30602.02 1000 ml 2-8°C
○SH30602.03 100 ml 2-8°C
MEM 培养基(Minimum Essential Medium)
EBSS*,含2.0 mM L-谷氨酰胺。 ●SH30024.01B 500 ml 2-8°C
●SH30024.FS 6 x 500 ml 2-8°C
●SH30024.02 1000 ml 2-8°C
●SH30024.LS 6 x 1000 ml 2-8°C
EBSS*,含L-谷氨酰胺,用于悬浮培养。 ○SH30235.01 500 ml 2-8°C
○SH30235.02 1000 ml 2-8°C
EBSS*,不含L-谷氨酰胺。 ●SH30244.01 500 ml 2-8°C
●SH30244.FS 6 x 500 ml 2-8°C
●SH30244.02 1000 ml 2-8°C
○SH30244.LS 6 x 1000 ml 2-8°C
α改良型,含L-谷氨酰胺、核糖核苷及脱氧核糖核苷。 ●SH30265.01B 500 ml 2-8°C
●SH30265.02 1000 ml 2-8°C
●SH30265.FS 6 x 500 ml 2-8°C
α改良型,不含L-谷氨酰胺、核糖核苷及脱氧核糖核苷。 ●SH30568.01 500 ml 2-8°C
○SH30568.FS 6 x 500 ml 2-8°C
○SH30568.02 1000 ml 2-8°C
Richter”s改良型,不含酚红。 ○SH30600.01 500 ml 2-8°C
○SH30600.02 1000 ml 2-8°C
Richter”s改良型,含L-谷氨酰胺、酚红。 ●SH30601.01 500 ml 2-8°C
○SH30601.02 1000 ml 2-8°C
用于悬浮培养,不含L-谷氨酰胺、氯化钙及镁。 ○SH30603.01 500 ml 2-8°C
○SH30603.02 1000 ml 2-8°C
RPMI 1640 培养基(RPMI 1640 Medium)
1X,含2.05 mM L-谷氨酰胺(同SH30091)。 ●SH30027.01 500 ml 2-8°C
●SH30027.FS 6 x 500 ml 2-8°C
●SH30027.02 1000 ml 2-8°C
●SH30027.LS 6 x 1000 ml 2-8°C
1X,含2.05 mM L-谷氨酰胺(同SH30027)。 ○SH30091.01 20 L袋装 2-8°C
○SH30091.02 10 L袋装 2-8°C
不含L-谷氨酰胺 ●SH30096.01 500 ml 2-8°C
●SH30096.FS 6 x 500 ml 2-8°C
●SH30096.02 1000 ml 2-8°C
○SH30096.LS 6 x 1000 ml 2-8°C
○SH30096.04 10 L袋装 2-8°C
○SH30096.05 20 L袋装 2-8°C
含HEPES,不含L-谷氨酰胺。 ○SH30203.05 500 ml 2-8°C
○SH30203.06 1000 ml 2-8°C
含L-谷氨酰胺、25 mM HEPES。 ●SH30255.01 500 ml 2-8°C
●SH30255.FS 6 x 500 ml 2-8°C
●SH30255.02 1000 ml 2-8°C
含L-谷氨酰胺、不含酚红。 ●SH30605.01 500 ml 2-8°C
○SH30605.02 1000 ml 2-8°C
含L-谷氨酰胺,不含硝酸钙。 ●SH30809.01B 500 ml 2-8°C
●SH30809.02B 1000 ml 2-8°C

货号标记说明:
“●” 表示常规生产产品。
“○” 表示按客户订单生产产品,有一定的最小定制量并需要一定时间。
“□” 表示未储存类项目,需要一段时间供给。
货号后标有“B”的 为赛默飞世尔生物化学制品(北京)有限公司生产,其他的为美国原产。
厚百商城提醒您:
所有液体培养基均经100纳米无菌过滤和出厂严格检测,除非需要,请不要私自进行二次过滤和任何形式的无菌检测。

ENDURO™迷你电泳电源

品牌:美国Labnet

订货号:E0304-230V

产品介绍

      Labnet全新设计的EnduroTM 迷你电泳电源是DNARNA、蛋白电泳和印迹转移电泳的理想选择。

      迷你电泳电源相比同系列的250V/300V电源,身材更小巧更节省实验室空间,拥有更高的性价比,可额外购买配套的电源箱,使实验更高效并节约空间。

         ENDURO™迷你电泳电源      ENDURO™迷你电泳电源

      电源的微处理器采用反馈回路信号控制以保持操作参数的稳定。 程序选择包括恒定的电压输出和恒定的电流输出,定时或连续的运行。设定值采用轻触薄膜键完成,数字显示。一些安全参数已经被设计到软件中包括自动断路、空载探测、瞬间载荷变化探测和过电压保护。如果发生断电,可以设置机器重启。2个输出终端可以允许2对不同的凝胶电泳同时进行。

 

型号

Model 250V

Model 300V

Mini

货号

E0203-230V

E0303-230V

E0304

输出类型

恒定电压或恒定电流,自动短路保护

恒定电压或恒定电流,自动短路保护

恒定电压或恒定电流,自动短路保护

输出电压范围/增量

5-250V/1V

2-300V/1V

10-300V/1V

输出电流范围/增量

10-3,000mA/10mA

4-500mA/1mA

10-400mA/1mA

输出功率范围/增量

1-300W/1W

90W max/NA

60W

定时器

1min99hr59min/连续

1min99hr59min/连续

1-999min带报警/连续

编程功能

有,10步编程,可存20个程序

显示 

液晶显示屏

液晶显示屏

液晶数字显示屏

可连接电泳系统数量

4

4

2

尺寸

19x25x8cm

19x25x8cm

14×19.1×8.cm

重量

2.5kg

2.2kg

1kg

输入电源

230V~50Hz

230V~50Hz

100-240V

 

  • 上海金畔生物科技有限公司

    文章号:558-558

Alpha淀粉酶[猪胰腺] α-Amylase (Porcine Pancreatic) 货号:E-PANAA-3G Megazyme中文站

Alpha淀粉酶[猪胰腺]

英文名:α-Amylase (Porcine Pancreatic)

货号:E-PANAA-3G

规格:3g

High purity alpha-Amylase (Porcine Pancreatic) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.1 
CAZy Family: GH13

From porcine pancreas. Partially purified. Free flowing powder.

Specific activity: ca. 100,000 U/g (40oC, Ceralpha reagent).

Stable at -20oC for > 4 years.

For use in total dietary fiber (including resistant starch) assay procedure.

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harlan teklad官网动物饲料代理商 他莫昔芬饮食 Tamoxifen diets TD.130855

harlan teklad官网动物饲料代理商

他莫昔芬饮食 harlan teklad官网动物饲料代理商 他莫昔芬饮食 Tamoxifen diets TD.130855

他莫昔芬激活Creer的饮食

有几篇文献引用了含有他莫昔芬的饮食对CRE模型在雌激素受体突变的控制下的应用。恩维戈特克乐饮食使各种含他莫昔芬的饮食定制,以满足您的研究需要。我们还制作了各种各样的含多西环素饮食对于被监管的系统。如果你有任何问题,请咨询营养师。

枸橼酸他莫昔芬和他莫昔芬

他莫昔芬作为他莫昔芬-蔗糖混合物添加到饮食中.鼓励用户使用库存供应。然而,泰克乐将继续与客户准备的混合。如果您选择此选项,请咨询营养师,以获得有关配制和发送混合物的更多信息。

鼓励用户从以下库存选项中进行选择,以便在几天内随时可供装运。

特克乐提供USP级三苯氧胺和枸橼酸他莫昔芬的饮食。
他莫昔芬
每公斤饲料毫克
日他莫昔芬
每公斤体重毫克1
饮食编码
(特克勒德供应他莫昔芬)
    天然色 红色
250 40 TD.130855 TD.130856
500 80 TD.130857 TD.130858
400柠檬酸盐2 40 TD.130859 TD.1308603

1假设体重20-25克,摄入量3-4克。
2枸橼酸他莫昔芬为66%他莫昔芬
3在欧洲称为TAM 400/Creer(TD.55125)

请为辐照饮食增加5-10天的准备时间;库存和定制产品至少需要3公斤订单。

这些饲料都有他莫昔芬与~5%蔗糖预混合,作为一种适口性增强剂;然而,饲料厌恶仍有可能发生。如果摄入是一个问题,请参阅下面的建议。

TeculadGlobal2016-这些例子中的基础饮食-仅仅是少数几种植物雌激素饮食中的一种。咨询营养学家关于其他基础饮食的使用。

折叠所有标签

  • 最低订货量为3公斤,足以喂养20只小鼠一个月。
  • 储存冷冻饮食,并计划在六个月内使用。
  • 典型的准备时间为两周(如果经过辐照,则为四周)
  • 辐照(可选)20 kGy的最低剂量(必须按顺序要求)
  • 订购:请致电800.483.5523或在线启动订单

计划:

  • 最初减少食物摄入量和减肥
  • 日常观察动物的耐受性-咨询您的IACUC监测指标。
  • 必要时进行干预

如果摄入似乎是一个问题:

  • 通过进行他莫昔芬给药试验,确定低剂量对你的模型是否有效。
  • 用含他莫昔芬颗粒与常规饲料颗粒混合逐渐驯服小鼠
  • 把食物弄湿,然后放在笼子里的盘子里(需要每天更换)。
  • 平日喂饲他莫昔芬饮食,周末有规律饮食。
  • 每隔一周(三苯氧胺饮食两周,休息一周)进行更长时间的治疗
  • 他莫昔芬是一种选择性雌激素受体调节剂(SERM),这意味着它可以抑制雌激素的作用或具有促进雌激素的作用。
  • 人体治疗效果的一般剂量为20毫克/天。用于啮齿动物的他莫昔芬饮食通常含有~1至2毫克/粒。
  • 当处理饮食时,可以使用典型的实验室预防措施,如实验室外套、手套和面罩,尽量减少接触他莫昔芬的事故。
  • 您的化学品安全部门应联系,以获得更多的机构特定的指导方针,以处理和处置含有他莫昔芬的饮食。
  • 治疗时间从一周到两周不等。1,2,5,6,7,8一至两个月3,4,8
  • 纯他莫昔芬1,5,8和枸橼酸他莫昔芬1,2,3,4,6,7都是有效的
  • 通常他莫昔芬剂量为每天40-80毫克/公斤体重。
  • 纯他莫昔芬的典型包合物为250 mg。8或500毫克1,5枸橼酸他莫昔芬为每公斤400毫克。1,2,3,4,6,7
  • 报告的初始重量损失为10%。1,2,4,7,8,与食物摄入量减少有关2
  • 恢复正常饮食后体重的恢复可能会因基因失活而受损。2,7
  1. 安德森·KB,Winer LH,Mork HK,Molkentin JD,Jaisser F.2010。他莫昔芬诱导CRE介导的小鼠心脏基因断裂的给药途径和剂量。 转基因RES 19:715-725。
  2. 蒋介明,凌杰,郑永赫,价格DL,Aja SM,Wong PC.2010年。TDP-43的缺失下调了与肥胖相关的基因Tbc1d1,并改变了身体的脂肪代谢。 美国Acad Sci美国:16320-16324。
  3. Kardakaris R.,Gareus R.,Xandomlea S,Pasparakis M.2011。内皮和巨噬细胞特异性P38αMAPK缺乏不影响ApoE-/-小鼠动脉粥样硬化的发病机制。 PLOSOne 6:e21055。
  4. [2]Kiermayer C,Conrad M,Schneider M,Schmidt J,Brielmeier M.枸橼酸他莫昔芬口服对小鼠心脏时空基因灭活的优化。 创世纪45:11-16。
  5. Koitabashi N,Bedja D,Zaiman AL,Pinto Ym,Zhang M,Gabrielson KL,Takimoto E,Kass DA。2009年。心肌细胞靶向他莫昔芬诱导MerCreMer基因缺失模型中短暂性心肌病的避免。 中国保监会第105:12-15号决议
  6. Kratsios P,Catela C,Salimova E,Huth M,Berno V,Rosenthal N,Morkioti F.2009。细胞自主Notch信号在胚胎和成年心脏心肌细胞中的独特作用。 PCirc第106:559-572号决议。
  7. Miro-Murillo M,Elorza A,Soro-ArNaiz I,Albacete-Albacete L,Ordonz A,Balsa E,Vara-Vega A,Vazquez S,Fuertes E,Fernandez-Criado C,Landazuri MO,Aragones J.2011。急性VHL基因失活可诱导心脏HIF依赖的促红细胞生成素基因表达. PLOS One 6:E 22589。
  8. Welle S,Burgess K,Thornton CA,Tawil R.2009。成熟小鼠肌生长抑素耗竭程度与肌肉生长的关系。 李玉玲.医学杂志.1997(4):E 935-40.

Tamoxifen diets

Diets with tamoxifen activate CreER

Several references cite use of tamoxifen-containing diets for models with Cre under the control of a mutated estrogen receptor. Envigo Teklad diets makes a variety of tamoxifen-containing diets customized to meet your research needs. We also make a variety of doxycycline-containing diets for tet regulated systems. If you have any questions, don’t hesitate to consult a nutritionist.

Teklad stocks tamoxifen and tamoxifen citrate

Tamoxifen is added to the diet as a tamoxifen-sucrose mixture. Users are encouraged to use the stocked supply. However, Teklad will continue to work with customer prepared mixes. If you choose this option, please consult a nutritionist for additional information about preparing and sending the mixture.

Users are encouraged to select from the stocked options below supply readily available to ship within days.

Teklad offers diet with USP grade tamoxifen and tamoxifen citrate
Tamoxifen
mg per kg diet
Daily tamoxifen
mg per kg body weight1
Diet code
(Teklad supplied tamoxifen)
    Natural colored Red
250 40 TD.130855 TD.130856
500 80 TD.130857 TD.130858
400 citrate2 40 TD.130859 TD.1308603

1 Assumes 20-25 g body weight and three-four g intake
2 Tamoxifen citrate is 66% tamoxifen
3 Referred to as TAM400/CreER in Europe (TD.55125)

Please add 5-10 day lead time for irradiated diets; minimum 3 kg orders are required for stocked and customized items.

These diets all have tamoxifen premixed with ~five percent sucrose as a palatability enhancer; however, feed aversion may still occur. If intake is a problem, see below for advice.

Teklad Global 2016 — base diet in these examples — is only one of several minimal phytoestrogen diets Teklad produces. Consult a nutritionist about use of other base diets.

COLLAPSE ALL TABS

  • Minimum order quantity is three kg, sufficient for feeding ~20 mice for one month
  • Store diet refrigerated and plan to use within six months
  • Typical lead time is two weeks (four weeks if irradiated)
  • Irradiation (optional) minimal dose of 20 kGy (Must be requested at time of order)
  • To place an order: contact Teklad Customer Service at 800.483.5523 or initiate order online

Plan for:

  • Initial reduction in food intake and weight loss
  • Daily observation of animal tolerance — consult your IACUC for monitoring metrics
  • Intervene when necessary

If intake seems to be a problem:

  • Determine if a lower dose is effective for your model by conducting a tamoxifen dose feeding trial
  • Gradually acclimate mice by mixing tamoxifen-containing pellets with regular feed pellets
  • Wet the food, then place in a dish inside the cage (requires daily replacement)
  • Feed tamoxifen diet on weekdays, regular diet on weekends
  • Alternate weeks (two weeks on tamoxifen diet, one week off) for longer treatments
  • Tamoxifen is a selective estrogen receptor modulator (SERM) meaning it can repress actions of estrogen or have pro-estrogen effects
  • Usual dose for therapeutic effects in humans is 20 mg/day. Tamoxifen diets for rodents typically contain ~one to two mg/pellet
  • Accidental tamoxifen exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
  • Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of tamoxifen containing diets.
  • Length of treatment varies from one to two weeks1,2,5,6,7,8 to one to two months3,4,8
  • Pure tamoxifen1,5,8 and tamoxifen citrate1,2,3,4,6,7 are both effective
  • Usual tamoxifen doses are ~40-80 mg per kg body weight per day
  • Typical inclusion for pure tamoxifen is 250 mg8 or 500 mg1,5 and for tamoxifen citrate is 400 mg per kg diet1,2,3,4,6,7
  • Initial weight loss of ten % is reported1,2,4,7,8, associated with reduced food intake2
  • Subsequent recovery of body weight after returning to regular diet may be compromised by gene inactivation2,7
  1. Andersson KB, Winer LH, Mork HK, Molkentin JD, Jaisser F. 2010. Tamoxifen administration routes and dosage for inducible Cre-mediated gene disruption in mouse hearts. Transgenic Res 19:715-725.
  2. Chiang PM, Ling J, Jeong YH, Price DL, Aja SM, Wong PC. 2010. Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism. Proc Natl Acad Sci USA 107:16320-16324.
  3. Kardakaris R, Gareus R, Xanthoulea S, Pasparakis M. 2011. Endothelial and macrophage-specific deficiency of P38alpha MAPK does not affect the pathogenesis of atherosclerosis in ApoE-/- mice. PLoS One 6:e21055.
  4. Kiermayer C, Conrad M, Schneider M, Schmidt J, Brielmeier M. 2007. Optimization of spatiotemporal gene inactivation in mouse heart by oral application of tamoxifen citrate. Genesis 45:11-16.
  5. Koitabashi N, Bedja D, Zaiman AL, Pinto YM, Zhang M, Gabrielson KL, Takimoto E, Kass DA. 2009. Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models. Circ Res 105:12-15.
  6. Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F. 2009. Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart. PCirc Res 106:559-572.
  7. Miro-Murillo M, Elorza A, Soro-Arnaiz I, Albacete-Albacete L, Ordonez A, Balsa E, Vara-Vega A, Vazquez S, Fuertes E, Fernandez-Criado C, Landazuri MO, Aragones J. 2011. Acute Vhl gene inactivation induces cardiac HIF-dependent erythropoietin gene expression. PLoS One 6:e22589.
  8. Welle S, Burgess K, Thornton CA, Tawil R. 2009. Relation between extent of myostatin depletion and muscle growth in mature mice. Am J Physiol Endocrinol Metab.Oct;297(4):E935-40.