GRAS Additive蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

GRAS Additive
GRAS Additive

GRAS Additive

GRAS Additive

GRAS Additive

GRAS Additive

Products > Optimization Screens > GRAS Additive > GRAS Additive

GRAS Additive

Applications

  • GRAS Additive™ is an optimization kit designed to evaluate 96 unique water soluble reagents and their ability to influence, promote and improve the crystallization of biological macromolecules.

Features

  • Developed at Hampton Research
  • Bio focused additive screen for the optimization of biological macromolecular crystals
  • For use with soluble proteins, membrane proteins, and biological therapeutics
  • Generally Recognized As Safe reagent formulation
  • Compatible with vapor diffusion, microbatch, free interface diffusion

Description

GRAS Additive is an optimization kit designed to allow rapid and convenient evaluation of 96 unique reagents and their ability to influence the crystallization of biological macromolecules, including but not limited to soluble proteins, membrane proteins, and biological therapeutics.1-5

The chemicals in GRAS Additive have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct.5, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

The 96 by 1 ml, deep well block reagent screen, is designed to be compatible with most popular crystallization reagents including reagents utilized in Hampton Research screens. Compatible with vapor diffusion, microbatch, and free interface diffusion. For research use only.

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GRAS Additive

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GRAS Additive
GRAS Additive
GRAS Additive

GRAS Additive

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GRAS Additive
GRAS Additive
GRAS Additive

CAT NO

HR2-459

NAME

GRAS Additive

DESCRIPTION

1 ml, Deep Well block format

PRICE

$545.00

cart quote

Support Material(s)

GRAS Additive HR2-459 GRAS Additive DocumentsGRAS Additive HR2-459 GRAS Additive SDSGRAS Additive GRAS Additive Formulation & Scoring Data

Related Item(S)

  • Individual GRAS Additive Reagents

References

1. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology 156 (2006) 387-406.

2. A novel strategy for the crystallization of proteins: X-ray diffraction validation. Steven B. Larson, John S. Day, Robert Cudney, and Alexander McPherson. Acta Cryst. (2007) D63, 310-318.

3. Development of an alternative approach to protein crystallization. McPherson, Alexander; Nguyen, Chieniang; Larson, Steven B; Day, John S; Cudney, Bob. J Struct Funct Genomics, Volume 8, Number 4, December 2007, 193-198.

4. Progress in the Development of an Alternative Approach to Macromolecular Crystallization. S. B. Larson,J. S. Day, C. Nguyen, R. Cudney, and A. McPherson. Crystal Growth & Design 2008 Volume 8, No. 8 3038-3052.

5. Wishart DS, Jewison T, Guo AC, Wilson M, Knox C, et al., HMDB 3.0 – The Human Metabolome Database in 2013. Nucleic Acids Res. 2013. Jan 1;41(D1):D801-7. 23161693.

6. Optimization of crystallization conditions for biological macromolecules. Alexander McPherson and Bob Cudney. Acta Crystallographica Section F Volume 70, Issue 11, pages 1445-1467, November 2014.

7. Screening and optimization strategies for macromolecular crystal growth. Cudney R, Patel S, Weisgraber K, Newhouse Y, McPherson A. Acta Crystallogr D Biol Crystallogr. 1994 Jul 1;50(Pt 4):414-23.

GRAS Additive GRAS Additive

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Heavy Atom Screens蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Heavy Atom Screens
Heavy Atom Screens

Heavy Atom Screens

Heavy Atom Screens

Heavy Atom Screens

Heavy Atom Screens

Products > Optimization Screens > Heavy Atom Screens > Heavy Atom Screens

Heavy Atom Screens

Applications

  • Heavy atoms for multiple isomorphous replacement

Features

  • Convenient sets of popular heavy atoms
  • Each kit include a heavy atom tutorial and formulation guide

Description

A Heavy Atom Screen kit provides 50 mg of each heavy atom in an o-ring screw cap micro tube. This convenient format provides sufficient material for the preparation of numerous (15 x 100 mM or 1,500 x 1 mM) small volume (0.1 ml) fresh stock solutions of heavy atoms. The quantity is sufficient for screening and derivatization but avoids the problem of storing large quantities of of heavy atoms.



Heavy Atom Screens

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Heavy Atom Screens

Heavy Atom Screens

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Heavy Atom Screens

CAT NO

HR2-442

NAME

Heavy Atom Screen Pt

DESCRIPTION

50 mg, tube format

PRICE

$587.00

cart quote

CAT NO

HR2-444

NAME

Heavy Atom Screen Au

DESCRIPTION

50 mg, tube format

PRICE

$403.00

cart quote

CAT NO

HR2-446

NAME

Heavy Atom Screen Hg

DESCRIPTION

50 mg, tube format

PRICE

$447.00

cart quote

CAT NO

HR2-448

NAME

Heavy Atom Screen M1

DESCRIPTION

50 mg, tube format

PRICE

$490.00

cart quote

CAT NO

HR2-450

NAME

Heavy Atom Screen M2

DESCRIPTION

50 mg, tube format

PRICE

$462.00

cart quote

Support Material(s)

Heavy Atom Screens HR2-442 Heavy Atom Screen Pt SDSHeavy Atom Screens HR2-444 Heavy Atom Screen Au SDSHeavy Atom Screens HR2-442 HR2-444 HR2-446 HR2-448 HR2-450 Heavy Atom Screens User GuideHeavy Atom Screens HR2-446 Heavy Atom Screen Hg SDSHeavy Atom Screens HR2-448 Heavy Atom Screen M1 SDSHeavy Atom Screens HR2-450 Heavy Atom Screen M2 SDS

Related Item(S)

  • Individual Heavy Atom Pt Reagents
  • Individual Heavy Atom Screen Hg Reagents
  • Individual Heavy Atom Screen M1 Reagents
  • Individual Heavy Atom M2 Reagents

References

1. Towards a rational approach for heavy-atom derivative screening in protein crystallography. Johnson Agniswamy, M. Gordon Joyce, Carl H. Hammer, and Peter D. Sun. Acta Cryst. (2008) D64, 354-367.

2. Heavy-atom derivatization. Elspeth Garman and James W. Murray, Acta Cryst. (2003). D59, 1903-1913.

3. Screening for phasing atoms in protein crystallography. Titus J Boggon and Lawrence Shapiro. Structure 2000, Vol 8 No 7, 143-149.

4. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part II: phasing of new structures. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1099-103. Epub 2002 Jun 20. Sun PD, Radaev S.

5. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part I: test cases. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1092-8. Epub 2002 Jun 20. Sun PD, Radaev S, Kattah M.

6. Preparation of isomorphous heavy-atom derivatives. Methods Enzymol. 1985;114:147-56. Petsko GA. PMID: 4079763

7. An overview of heavy-atom derivatization of protein crystals. A. C. W. Pike, E. F. Garman, T. Krojer, F. von Delft & E. P. Carpenter (2016). Acta Cryst. D72, 303-318.

8. Fluorescence Detection of Heavy Atom Labeling (FD-HAL): a rapid method for identifying covalently modified cysteine residues by phasing atoms.Chaptal V, Ujwal R, Nie Y, Watanabe A, Kwon S, Abramson J. J Struct Biol. 2010 Jul;171(1):82-7.

9. A rapid and rational approach to generating isomorphous heavy-atom phasing derivatives. Lu J, Sun PD. FEBS J. 2014 Sep;281(18):4021-8.

10. A rational approach to heavy-atom derivative screening. Joyce MG, Radaev S, Sun PD. Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):358-65.

Heavy Atom Screens Heavy Atom Screens

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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  • Contact Us
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© 2022 HAMPTON RESEARCH CORP.


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可熔性聚四氟乙烯(PFA)螺旋管(称呼:SCFTA-0.75×4.0、m/卷:20)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)螺旋管(称呼:SCFTA-0.75×4.0、m/卷:20)
产品编号: WEB20152 价格: 会员价:0元;市场价:0元 产品特点
防止、结束各种电线的磨损或损伤
产品规格
材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)螺旋管(称呼:SCFTA-0.75×4.0、m/卷:20)可熔性聚四氟乙烯(PFA)螺旋管(称呼:SCFTA-0.75×4.0、m/卷:20)产品特征: ,  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA平底容器0104(容量:120ml)日本三博特sanplatec

PFA平底容器0104(容量:120ml)
产品编号: WEB15282 价格: 会员价:0元;市场价:0元 产品特点
使用温度范围:-196~200℃
产品规格

整体尺寸(mm)(外径×内径×高):49×  44×120

瓶盖尺寸(mm)(外径×高):59×124

材料

确认材料的耐药性 >> 耐药性检索

        PFA平底容器0104(容量:120ml)PFA平底容器0104(容量:120ml)产品特征

.特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)螺旋管(称呼:SCFTA-0.5×2.6、m/卷:20)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)螺旋管(称呼:SCFTA-0.5×2.6、m/卷:20)
产品编号: WEB20151 价格: 会员价:0元;市场价:0元 产品特点
防止、结束各种电线的磨损或损伤
产品规格
材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)螺旋管(称呼:SCFTA-0.5×2.6、m/卷:20)可熔性聚四氟乙烯(PFA)螺旋管(称呼:SCFTA-0.5×2.6、m/卷:20)产品特征: ,  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA平底容器0103(容量:90ml)日本三博特sanplatec

PFA平底容器0103(容量:90ml)
产品编号: WEB15281 价格: 会员价:0元;市场价:0元 产品特点
使用温度范围:-196~200℃
产品规格

整体尺寸(mm)(外径×内径×高):66×  61×  49

带瓶盖尺寸(mm)(外径×高):76×  53

材料

确认材料的耐药性 >> 耐药性检索

        PFA平底容器0103(容量:90ml)PFA平底容器0103(容量:90ml)产品特征

特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 22×25×1.5)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 22×25×1.5)
产品编号: WEB10866 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

规格(外径×内径×厚度):22×25×1.5

最小弯曲半径 :370

破坏压力:19

长度:10m/卷

材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 22×25×1.5)可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 22×25×1.5)产品特征

●特点
在之前的 PFA管的性能上有所增加, 有以下的特点。
 ・ 使用新的 PFA有优良的纯染性、 减少离子洗脱。 ・ 管内表面平滑 (Rt=0.2µm)。 (Rt=Rmax:最大高度 ) ・ 减少粒子和药液的滞留。
・ 透明性增加。
・ 绝缘性增加
・ 管内表面积减少降低药液浸透层。
・ 受力的情况下耐应力开裂性提高 (例如 : 硫酸过水、 发烟硫酸 )

  单位 PFA-HG A公司 B公司
表面粗糙程度 (Rt) (μm) 0.2 0.8 0.8
※上述数值是实际测定值, 不是规格值。
※Rt=Rmax
※A公司、 B公司是普通的 PFA管。
金属离子洗脱结果
项目 洗脱重量
K 0.02
Na 0.01
Ca 0.01
Al 0.02
Cr 0.01
Ni 0.01
Fe 0.02
Cu 0.01
<分析方法>
①切断样本 PFA-HG管 (10×12)1m,用水清洗干净切口。
②充填大约 70ml氢氟酸到样本中,室温下进行6天的洗脱试验。
③洗脱试验结束后,蒸干结晶洗脱液,往残留物
中加入硝酸后用去离子水进行稀释。furesu原
子吸收光谱法测定洗脱液里含有成分的绝对含量。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

Index • Index HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Index • Index HT
Index • Index HT

Index • Index HT

Index • Index HT

Index • Index HT

Index • Index HT

Products > Crystallization Screens > Index > Index • Index HT

Index • Index HT

Applications

  • Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules

Features

  • Developed at Hampton Research
  • A data-driven biased sparse matrix and grid screen
  • Screens classic, contemporary, & modern crystallization reagents
  • Samples pH 3 to 9
  • Compatible with microbatch, vapor diffusion, & liquid diffusion methods
  • Specially formulated reagent zones:
    • Traditional salts versus pH
    • Neutralized organic acids
    • High [salt] with low [polymer]
    • High [polymer] with low [salt]
    • Low ionic strength versus pH
    • PEG & Salt versus pH
    • PEG & Salt
  • Tube or Deep Well block format

Description

Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format. 

Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in producing crystals or limiting sample solubility. Results from Index can be used to design optimization experiments and to identify follow on screens by reagent class. For example, positive results with salt based reagents in Index may be followed up with further screening using SaltRx or Grid Screen Salt HT. Success with polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.

Index utilizes a broad, yet refined portfolio of crystallization reagent systems. These include the following: (1) traditional salts such as Ammonium sulfate and Sodium chloride versus pH; (2) neutralized organic acids such as Sodium malonate and Tacsimate; (3) High salt concentration mixed with low polymer concentration as well as high polymer concentration mixed with low salt concentration and; (4) Low ionic strength using polymers such as PEG, MPD, Pentaerythritols versus pH. These reagent systems are formulated across a sparse matrix and incomplete factorial of concentration ranges, sampling a pH range of 3 to 9.

Index contains 96 unique reagents, 10 ml each.

Index HT contains 96 unique reagents in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Measured pH range of kit is 3 to 9 at 25°C
Average measured pH of kit is 6.8 at 25°C
Median measured pH of kit is 6.9 at 25°C

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Index • Index HT

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Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT

Index • Index HT

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Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT
Index • Index HT

CAT NO

HR2-144

NAME

Index

DESCRIPTION

10 ml, tube format

PRICE

$550.00

cart quote

CAT NO

HR2-134

NAME

Index HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

Index • Index HT HR2-144 Index DocumentsIndex • Index HT HR2-144 Index SDSIndex • Index HT HR2-134 Index HT DocumentsIndex • Index HT HR2-134 Index HT SDSIndex • Index HT Index & Index HT Formulations & Scoring

Related Item(S)

  • Individual Index • Index HT Reagents

References

1. The advantages of using a modified microbatch method for rapid screening of protein crystallization conditions. A. D′Arcy, A. Mac Sweeney, M. Stihle and A. Haber. Acta Cryst. (2003). D59, 396-399.

2. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of propionate kinase (TdcD) from Salmonella typhimurium. D. K. Simanshu and M. R. N. Murthy. Acta Cryst. (2005). F61, 52-55.

3. Preparation, crystallization and preliminary X-ray analysis of the methionine synthase (MetE) from Streptococcus mutans. T.-M. Fu, X.-Y. Zhang, L.-F. Li, Y.-H. Liang and X.-D. Su. Acta Cryst. (2006). F62, 984-985.

4. Crystallization and preliminary X-ray analysis of glutathione transferases from cyanobacteria. S. C. Feil, J. Tang, G. Hansen, M. A. Gorman, E. Wiktelius, G. Stenberg and M. W. Parker. Acta Cryst. (2009). F65, 475-477.

Index • Index HT Index • Index HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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PFA平底容器0102(容量:60ml)日本三博特sanplatec

PFA平底容器0102(容量:60ml)
产品编号: WEB15280 价格: 会员价:0元;市场价:0元 产品特点
使用温度范围:-196~200℃
产品规格

整体尺寸(mm)(外径×内径×高):49×  44×  62

瓶盖尺寸(mm)(外径×高):59×  66

材料

确认材料的耐药性 >> 耐药性检索

        PFA平底容器0102(容量:60ml)PFA平底容器0102(容量:60ml)产品特征

特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):16×19×1.5)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):16×19×1.5)
产品编号: WEB10865 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

规格(外径×内径×厚度):16×19×1.5

最小弯曲半径:200

破坏压力:24

长度:10m/卷

材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):16×19×1.5)可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):16×19×1.5)产品特征

●特点
在之前的 PFA管的性能上有所增加, 有以下的特点。
 ・ 使用新的 PFA有优良的纯染性、 减少离子洗脱。 ・ 管内表面平滑 (Rt=0.2µm)。 (Rt=Rmax:最大高度 ) ・ 减少粒子和药液的滞留。
・ 透明性增加。
・ 绝缘性增加
・ 管内表面积减少降低药液浸透层。
・ 受力的情况下耐应力开裂性提高 (例如 : 硫酸过水、 发烟硫酸 )

  单位 PFA-HG A公司 B公司
表面粗糙程度 (Rt) (μm) 0.2 0.8 0.8
※上述数值是实际测定值, 不是规格值。
※Rt=Rmax
※A公司、 B公司是普通的 PFA管。
金属离子洗脱结果
项目 洗脱重量
K 0.02
Na 0.01
Ca 0.01
Al 0.02
Cr 0.01
Ni 0.01
Fe 0.02
Cu 0.01
<分析方法>
①切断样本 PFA-HG管 (10×12)1m,用水清洗干净切口。
②充填大约 70ml氢氟酸到样本中,室温下进行6天的洗脱试验。
③洗脱试验结束后,蒸干结晶洗脱液,往残留物
中加入硝酸后用去离子水进行稀释。furesu原
子吸收光谱法测定洗脱液里含有成分的绝对含量。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA平底容器0202(容量:60ml)日本三博特sanplatec

PFA平底容器0202(容量:60ml)
产品编号: WEB15279 价格: 会员价:0元;市场价:0元 产品特点
使用温度范围:-196~200℃
产品规格

整体尺寸(mm)(外径×内径×高):49×  44×  43

瓶盖尺寸(mm)(外径×高):59×  47

材料

确认材料的耐药性 >> 耐药性检索

        PFA平底容器0202(容量:60ml)PFA平底容器0202(容量:60ml)产品特征

特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):10×12×1.0)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):10×12×1.0)
产品编号: WEB10864 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

规格(外径×内径×厚度):10×12×1.0

最小弯曲半径 :130

破坏压力:25

长度:10m/卷

材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):10×12×1.0)可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):10×12×1.0)产品特征

●特点
在之前的 PFA管的性能上有所增加, 有以下的特点。
 ・ 使用新的 PFA有优良的纯染性、 减少离子洗脱。 ・ 管内表面平滑 (Rt=0.2µm)。 (Rt=Rmax:最大高度 ) ・ 减少粒子和药液的滞留。
・ 透明性增加。
・ 绝缘性增加
・ 管内表面积减少降低药液浸透层。
・ 受力的情况下耐应力开裂性提高 (例如 : 硫酸过水、 发烟硫酸 )

  单位 PFA-HG A公司 B公司
表面粗糙程度 (Rt) (μm) 0.2 0.8 0.8
※上述数值是实际测定值, 不是规格值。
※Rt=Rmax
※A公司、 B公司是普通的 PFA管。
金属离子洗脱结果
项目 洗脱重量
K 0.02
Na 0.01
Ca 0.01
Al 0.02
Cr 0.01
Ni 0.01
Fe 0.02
Cu 0.01
<分析方法>
①切断样本 PFA-HG管 (10×12)1m,用水清洗干净切口。
②充填大约 70ml氢氟酸到样本中,室温下进行6天的洗脱试验。
③洗脱试验结束后,蒸干结晶洗脱液,往残留物
中加入硝酸后用去离子水进行稀释。furesu原
子吸收光谱法测定洗脱液里含有成分的绝对含量。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA平底容器0201(容量:33ml)日本三博特sanplatec

PFA平底容器0201(容量:33ml)
产品编号: WEB15278 价格: 会员价:0元;市场价:0元 产品特点
使用温度范围:-196~200℃
产品规格

整体尺寸(mm)(外径×内径×高):29×  25×129

瓶盖尺寸(mm)(外径×高):36×133

材料

确认材料的耐药性 >> 耐药性检索

        PFA平底容器0201(容量:33ml)PFA平底容器0201(容量:33ml)产品特征

特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):8×10×1.0)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):8×10×1.0)
产品编号: WEB10863 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

规格(外径×内径×厚度):8×10×1.0

最小弯曲半径:80

破坏压力:33

长度:10m/卷

材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):8×10×1.0)可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):8×10×1.0)产品特征

●特点
在之前的 PFA管的性能上有所增加, 有以下的特点。
 ・ 使用新的 PFA有优良的纯染性、 减少离子洗脱。 ・ 管内表面平滑 (Rt=0.2µm)。 (Rt=Rmax:最大高度 ) ・ 减少粒子和药液的滞留。
・ 透明性增加。
・ 绝缘性增加
・ 管内表面积减少降低药液浸透层。
・ 受力的情况下耐应力开裂性提高 (例如 : 硫酸过水、 发烟硫酸 )

  单位 PFA-HG A公司 B公司
表面粗糙程度 (Rt) (μm) 0.2 0.8 0.8
※上述数值是实际测定值, 不是规格值。
※Rt=Rmax
※A公司、 B公司是普通的 PFA管。
金属离子洗脱结果
项目 洗脱重量
K 0.02
Na 0.01
Ca 0.01
Al 0.02
Cr 0.01
Ni 0.01
Fe 0.02
Cu 0.01
<分析方法>
①切断样本 PFA-HG管 (10×12)1m,用水清洗干净切口。
②充填大约 70ml氢氟酸到样本中,室温下进行6天的洗脱试验。
③洗脱试验结束后,蒸干结晶洗脱液,往残留物
中加入硝酸后用去离子水进行稀释。furesu原
子吸收光谱法测定洗脱液里含有成分的绝对含量。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

Ionic Liquid Screen蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Ionic Liquid Screen
Ionic Liquid Screen

Ionic Liquid Screen

Ionic Liquid Screen

Ionic Liquid Screen

Ionic Liquid Screen

Products > Optimization Screens > Ionic Liquid Screen > Ionic Liquid Screen

Ionic Liquid Screen

Applications

  • Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility
  • Additive screen for protein crystallization

Features

  • 24 unique ionic liquids
  • 0.5 ml tube format

Description

Ionic Liquid Screen is a kit designed to allow the rapid and convenient evaluation of 24 unique ionic liquids and their ability to influence the crystallization of the sample. The screen is designed to be compatible with most crystallization reagents including all reagents utilized in all of the Hampton Research screens.

Ionic liquids have been found effective as additives in protein crystallization, with different ionic liquids used to increase crystallization rates and crystal size.1-4 The inclusion of ionic liquids in crystallization experiments has been reported to lead to less crystal polymorphism as well as less precipitation at higher precipitant concentrations.25 Ionic liquids have been used as additives to produce crystals in reagents that had previously not resulted in crystallization and results suggest ionic liquids may be applicable for the solubilization and crystallization of membrane proteins.2

Ionic liquids are organic salts with melting points below 100°Celsius. They are thermally stable, nonflammable and demonstrate very low vapor pressure. Ionic liquids are soluble in a variety of organic and inorganic reagents and can be highly water soluble. Ionic liquids can demonstrate a degree of localized structuring about each ion compared to materials composed of disassociated ions, setting them apart from salt solutions.5-6 Ionic liquids can participate in ionic, hydrophobic and hydrogen bond interactions. Ionic liquids are often chaotropic, composed of low symmetry ions with charge delocalization and weak intermolecular interactions.1 These organic salts generally consist of combinations of organic cations and either an organic or inorganic anion. Ionic liquids have been demonstrated to suppress protein aggregation and significantly increase protein folding yields.7-8 Ionic liquids have been reported to stabilize protein activity and structure.9-11 The inclusion of the ionic liquid 1-n-Butyl-3-methylimidazolium tetrafluoroborate (included in this kit) improved the thermal stability and solubility of integral membrane proteins for membrane proteomics study.12

Some ionic liquids, such as ethylammonium nitrate have water-like characteristics, including the capacity for hydrogen bonding and the promotion of micelle formation by some surfactants.13 Many ionic liquids are also organics acids and have ionic character in addition to the hydrophobic behavior, which makes them unique and useful solvents in protein chemistry.

Variation of the anion and the cation as well as the utilization of both soft (formate and acetate) and hard anions (nitrate) in the Ionic Liquid Screen reagents provides an additional dimension for evaluating the effects on ionic liquids on the solubility and crystallization of proteins. The Ionic Liquid Screen contains a set of 24 water soluble ionic liquids that comprise different cation (imidazolium, phosphonium, ammonium) and anion (borate, halides, sulfates, acetates, sulfonates, nitrates) structures for a diverse ionic liquid additive screen for use in improving the crystallization behavior and X-ray diffraction resolution of proteins.

Each of the 24 ionic liquids are preformulated in deionized water and sterile filtered using a 0.2 micron filter. Ionic liquid Screen contains 24 unique reagents, 0.5 milliliter each.

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Ionic Liquid Screen

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Ionic Liquid Screen
Ionic Liquid Screen
Ionic Liquid Screen

Ionic Liquid Screen

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Ionic Liquid Screen
Ionic Liquid Screen
Ionic Liquid Screen

CAT NO

HR2-214

NAME

Ionic Liquid Screen

DESCRIPTION

0.5 ml, tube format

PRICE

$310.00

cart quote

Support Material(s)

Ionic Liquid Screen HR2-214 Ionic Liquid Screen DocumentsIonic Liquid Screen HR2-214 Ionic Liquid SDSIonic Liquid Screen Ionic Liquid Screen Formulation and Scoring Data

Related Item(S)

  • Individual Ionic Liquid Reagents

References

1. Pusey, M.L., Paley, M.S., Turner, M.B., and Rogers, R.D. 2007. Protein crystallization using room temperature ionic liquids. Crystal Growth & Design. 74:787-793.

2. Hekmat, D., Hebel, D., Sebastian, J. Schmidt, M., and Weuster-Botz, D. 2007. Advanced protein crystallization using water-soluble ionic liquids as crystallization additives. Biotech. Lett., 29:703-1711.

3. Garlitz, J.A., Summers, C.A., Flowers, R.A. and Borgstahl, G.E.O. 1999. Ethylammonium nitrate: A protein crystallization reagent. Acta Cryst. D53:2037-2038.

4. Judge, R.A., Takahashi, S., Longnecker, K.L. Fry, E.H., Abad-Zapatero, C., and Chi, M.L. 2009. The effects of ionic liquids on protein crystallization and X-ray diffraction resolution. Crystal Growth & Design 9:3463-3469.

5. Bowran, D.T., Hardacre, C. Holbrey, J.D., McMath, J.E., and Soper, A.K. 2003. J. Chem. Phys. 118:173-178.

6. Cadena, C., Zhao, Q., Snurr, and R.Q., Maginn, E.J. 2006. J. Chem. Phys. B 110:2821-2832.

7. Summers, C.A. and Flowers, R.A. 2000. Protein renaturation by the liquid organic salt ethylammonium nitrate. Protein Science 9:2001-2008.

8. Lange, C., Patil, G., and Rudolph, R. 2005. Ionic liquids as refolding additives: N′-alkyl and N′-(w-hydroxyalkyl) N-methylimidazolium chloride. Protein Science 14:2693-2701.

9. Lozano, P. , de Diego, T. Guegan, J.P., Vaultier, M., and Ibora, J.L. 2001. Stabilization of a-chymotrypsin by ionic liquids in transesterification reactions. Biotehnol. Bioeng. 75:363-369.

10. Baker, S.N., McCleskey, T.M., Pandy, S., and Baker, G.A. 2004. Fluorescence study of protein thermostability in ionic liquids. Chem. Commun. 2004:940-941.

11. De Diego, T., Lozano, P., Gmouth, S. Vaultier, M., and Iborra, J.L. 2004. Fluorescence and CD spectroscopic analysis of the a-chymotrypsin stabilization by the ionic liquid 1-ethyl-3-methyimidazolium bis[(trifluormethyl)sulfonyl]amide. Biotechnol. Bioeng. 88:916-924.

12. Sun, L., Tao, D., Han, B., Ma, J., Zhu, G., Liang, Z., Shan, Y., Zhang, L., and Zhang, Y. 2010. Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics. Anal. Bioanal. Chem. DOI 10.1007/s0216-010-4381-5.

13. Evans, D.F., Yamauchi, A., Roman, R., and Casassa, E.Z. 1982. J. Colloids Interface Sci. 88:89-96.

14. Proteins in Ionic Liquids: Current Status of Experiments and Simulations. Christian Schrode, Top Curr Chem (J). 2017; 375(2): 25.

15. Ionic Liquids as Stabilization and Refolding Additives and Solvents for Proteins. Fujita K. Adv Biochem Eng Biotechnol. 2018 Jul 13. doi: 10.1007/10_2018_65.

16. Fujita K. (2018) Ionic Liquids as Stabilization and Refolding Additives and Solvents for Proteins. In: . Advances in Biochemical Engineering/Biotechnology. Springer, Berlin, Heidelberg.

Ionic Liquid Screen Ionic Liquid Screen

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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PFA平底容器0275(容量:23ml)日本三博特sanplatec

PFA平底容器0275(容量:23ml)
产品编号: WEB15277 价格: 会员价:0元;市场价:0元 产品特点
使用温度范围:-196~200℃
产品规格

整体尺寸(mm)(外径×内径×高):29×  25×  66

带瓶盖尺寸(mm)(外径×高):36×  70

材料

确认材料的耐药性 >> 耐药性检索

        PFA平底容器0275(容量:23ml)PFA平底容器0275(容量:23ml)产品特征

特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 6×8×1.0)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 6×8×1.0)
产品编号: WEB10862 价格: 会员价:0元;市场价:0元 产品特点
加上原来可熔性聚四氟乙烯(PFA)管的性能,有以下特点。
产品规格

规格(外径×内径×厚度):6×  8×1.0

最小弯曲半径:50

破坏压力:42

长度:10m/卷

材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 6×8×1.0)可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 6×8×1.0)产品特征

●特点
在之前的 PFA管的性能上有所增加, 有以下的特点。
 ・ 使用新的 PFA有优良的纯染性、 减少离子洗脱。 ・ 管内表面平滑 (Rt=0.2µm)。 (Rt=Rmax:最大高度 ) ・ 减少粒子和药液的滞留。
・ 透明性增加。
・ 绝缘性增加
・ 管内表面积减少降低药液浸透层。
・ 受力的情况下耐应力开裂性提高 (例如 : 硫酸过水、 发烟硫酸 )

  单位 PFA-HG A公司 B公司
表面粗糙程度 (Rt) (μm) 0.2 0.8 0.8
※上述数值是实际测定值, 不是规格值。
※Rt=Rmax
※A公司、 B公司是普通的 PFA管。
金属离子洗脱结果
项目 洗脱重量
K 0.02
Na 0.01
Ca 0.01
Al 0.02
Cr 0.01
Ni 0.01
Fe 0.02
Cu 0.01
<分析方法>
①切断样本 PFA-HG管 (10×12)1m,用水清洗干净切口。
②充填大约 70ml氢氟酸到样本中,室温下进行6天的洗脱试验。
③洗脱试验结束后,蒸干结晶洗脱液,往残留物
中加入硝酸后用去离子水进行稀释。furesu原
子吸收光谱法测定洗脱液里含有成分的绝对含量。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA平底容器0220(容量:20ml)日本三博特sanplatec

PFA平底容器0220(容量:20ml)
产品编号: WEB15276 价格: 会员价:0元;市场价:0元 产品特点
使用温度范围:-196~200℃
产品规格

整体尺寸(mm)(外径×内径×高):29×  25×  51

瓶盖尺寸(mm)(外径×高):36×  55

材料

确认材料的耐药性 >> 耐药性检索

        PFA平底容器0220(容量:20ml)PFA平底容器0220(容量:20ml)产品特征

特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 4×6×1.0)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 4×6×1.0)
产品编号: WEB10861 价格: 会员价:0元;市场价:0元 产品特点
加上原来可熔性聚四氟乙烯(PFA)管的性能,有以下特点。
产品规格

规格(外径×内径×厚度):4×  6×1.0

最小弯曲半径:25

破坏压力:55

长度:10m/卷

材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 4×6×1.0)可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度): 4×6×1.0)产品特征

●特点
在之前的 PFA管的性能上有所增加, 有以下的特点。
 ・ 使用新的 PFA有优良的纯染性、 减少离子洗脱。 ・ 管内表面平滑 (Rt=0.2µm)。 (Rt=Rmax:最大高度 ) ・ 减少粒子和药液的滞留。
・ 透明性增加。
・ 绝缘性增加
・ 管内表面积减少降低药液浸透层。
・ 受力的情况下耐应力开裂性提高 (例如 : 硫酸过水、 发烟硫酸 )

  单位 PFA-HG A公司 B公司
表面粗糙程度 (Rt) (μm) 0.2 0.8 0.8
※上述数值是实际测定值, 不是规格值。
※Rt=Rmax
※A公司、 B公司是普通的 PFA管。
金属离子洗脱结果
项目 洗脱重量
K 0.02
Na 0.01
Ca 0.01
Al 0.02
Cr 0.01
Ni 0.01
Fe 0.02
Cu 0.01
<分析方法>
①切断样本 PFA-HG管 (10×12)1m,用水清洗干净切口。
②充填大约 70ml氢氟酸到样本中,室温下进行6天的洗脱试验。
③洗脱试验结束后,蒸干结晶洗脱液,往残留物
中加入硝酸后用去离子水进行稀释。furesu原
子吸收光谱法测定洗脱液里含有成分的绝对含量。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

PFA平底容器0225(容量:7ml)日本三博特sanplatec

PFA平底容器0225(容量:7ml)
产品编号: WEB15274 价格: 会员价:0元;市场价:0元 产品特点
使用温度范围:-196~200℃
产品规格

整体尺寸(mm)(外径×内径×高):22×  17×  32

瓶盖尺寸(mm)(外径×高):27×  36

材料

确认材料的耐药性 >> 耐药性检索

        PFA平底容器0225(容量:7ml)PFA平底容器0225(容量:7ml)产品特征

特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

MembFac • Crystal Screen Lite • MembFac HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

Products > Crystallization Screens > MembFac • Crystal Screen Lite > MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

Applications

  • Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility

Features

  • Membrane protein sparse matrix screens
  • Sparse matrix formula efficiently samples salts, polymers, organics and pH
  • pH range 4.6 – 8.5
  • Formulated for use with detergents
  • Tube or Deep Well block format
  • Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

Description

MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins.

MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.

MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.

The Rationale The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.

The Method The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.

The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.

Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2 Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.

MembFac contains 48 unique reagents, 10 ml each.

MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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MembFac • Crystal Screen Lite • MembFac HT

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MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT

MembFac • Crystal Screen Lite • MembFac HT

Click to Zoom In

MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT
MembFac • Crystal Screen Lite • MembFac HT

CAT NO

HR2-114

NAME

MembFac

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-128

NAME

Crystal Screen Lite

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-137

NAME

MembFac HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

MembFac • Crystal Screen Lite • MembFac HT HR2-114 MembFac DocumentsMembFac • Crystal Screen Lite • MembFac HT HR2-114 MembFac SDSMembFac • Crystal Screen Lite • MembFac HT HR2-128 Crystal Screen Lite DocumentsMembFac • Crystal Screen Lite • MembFac HT HR2-128 Crystal Screen Lite SDSMembFac • Crystal Screen Lite • MembFac HT HR2-137 MembFac HT DocumentsMembFac • Crystal Screen Lite • MembFac HT HR2-137 MembFac HT SDSMembFac • Crystal Screen Lite • MembFac HT MembFac & Crystal Screen Lite Formulation & Scoring Data

Related Item(S)

  • Individual MembFac • Crystal Screen Lite • MembFac HT Reagents

References

1. UCLA Crystallization Workshop, June 21, 1993.

2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.

3. McPherson, A., Current approaches to macromolecular crystallization., Eur. J. Biochem. (1990) 189, 1-23.

4. Jancarik, J. and Kim, S.H., Sparse Matrix Sampling: a screening method for crystallization of proteins. (1991) J. Appl. Cryst., 24,409-411.

5. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.

6. Structural and functional characterization of the N-terminal domain of the yeast Mg2+ channel Mrs2. M. B. Khan, G. Sponder, B. Sjoblom, S. Svidova, R. J. Schweyen, O. Carugo and K. Djinovic-Carugo. Acta Cryst. (2013). D69, 1653-1664.

MembFac • Crystal Screen Lite • MembFac HT MembFac • Crystal Screen Lite • MembFac HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):2×4×1.0)日本三博特sanplatec

可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):2×4×1.0)
产品编号: WEB10860 价格: 会员价:0元;市场价:0元 产品特点
加上原来可熔性聚四氟乙烯(PFA)管的性能,有以下特点。
产品规格

规格(外径×内径×厚度):2×  4×1.0

最小弯曲半径 :15

破坏压力:90

长度:10m/卷

材料

确认材料的耐药性 >> 耐药性检索

        可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):2×4×1.0)可熔性聚四氟乙烯(PFA)-HG管(规格(内径×外径×厚度):2×4×1.0)产品特征

●特点
在之前的 PFA管的性能上有所增加, 有以下的特点。
 ・ 使用新的 PFA有优良的纯染性、 减少离子洗脱。 ・ 管内表面平滑 (Rt=0.2µm)。 (Rt=Rmax:最大高度 ) ・ 减少粒子和药液的滞留。
・ 透明性增加。
・ 绝缘性增加
・ 管内表面积减少降低药液浸透层。
・ 受力的情况下耐应力开裂性提高 (例如 : 硫酸过水、 发烟硫酸 )

  单位 PFA-HG A公司 B公司
表面粗糙程度 (Rt) (μm) 0.2 0.8 0.8
※上述数值是实际测定值, 不是规格值。
※Rt=Rmax
※A公司、 B公司是普通的 PFA管。
金属离子洗脱结果
项目 洗脱重量
K 0.02
Na 0.01
Ca 0.01
Al 0.02
Cr 0.01
Ni 0.01
Fe 0.02
Cu 0.01
<分析方法>
①切断样本 PFA-HG管 (10×12)1m,用水清洗干净切口。
②充填大约 70ml氢氟酸到样本中,室温下进行6天的洗脱试验。
③洗脱试验结束后,蒸干结晶洗脱液,往残留物
中加入硝酸后用去离子水进行稀释。furesu原
子吸收光谱法测定洗脱液里含有成分的绝对含量。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

33ml PFA圆底容器 (外径×内径×全高(mm) : 29×25×67H)日本三博特sanplatec

33ml PFA圆底容器 (外径×内径×全高(mm) : 29×25×67H)
产品编号: WEB15271 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

本体尺寸(mm)×外径×内径×高:29Φ×25Φ×67

瓶盖尺寸(mm)×外径×高:36Φ×70

材料

确认材料的耐药性 >> 耐药性检索

        33ml PFA圆底容器 (外径×内径×全高(mm) : 29×25×67H)33ml PFA圆底容器 (外径×内径×全高(mm) : 29×25×67H)产品特征

特点 :瓶子完全由PFA制造 ,耐药品性优越 。金属溶出及少 。作为无金属容 器,适合保管各种分析试料。内部形状为圆形,可以简单取出内容液。 耐热性,耐寒性优越。可在冷冻库冷冻,也可在油浴加热。可可用于广泛的温度领域(-196℃~250℃)。本品为非粘性,适合操作需要剥离或处理高粘度的样品。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1×7/8(英寸))日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1×7/8(英寸))
产品编号: WEB10827 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

PFA管 (直管 )1×7/8(英寸)

规格(外径×内径×厚度):25.4  ×22.22×1.59

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1×7/8(英寸))NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1×7/8(英寸))产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

22ml PFA圆底容器 (外径×内径×全高mm:29×25×51H)日本三博特sanplatec

22ml PFA圆底容器 (外径×内径×全高mm:29×25×51H)
产品编号: WEB15270 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

本体尺寸(mm)×外径×内径×高:29Φ×25Φ×51

瓶盖尺寸(mm)×外径×高:36Φ×55

材料

确认材料的耐药性 >> 耐药性检索

        22ml PFA圆底容器 (外径×内径×全高mm:29×25×51H)22ml PFA圆底容器 (外径×内径×全高mm:29×25×51H)产品特征

特点 :瓶子完全由PFA制造 ,耐药品性优越 。金属溶出及少 。作为无金属容 器,适合保管各种分析试料。内部形状为圆形,可以简单取出内容液。 耐热性,耐寒性优越。可在冷冻库冷冻,也可在油浴加热。可可用于广泛的温度领域(-196℃~250℃)。本品为非粘性,适合操作需要剥离或处理高粘度的样品。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/4×5/8(英寸))日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/4×5/8(英寸))
产品编号: WEB10826 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

PFA管 (直管 )3/4×5/8(英寸)

规格(外径×内径×厚度):19.05×15.87×1.59

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/4×5/8(英寸))NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 3/4×5/8(英寸))产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

15ml PFA圆底容器 (外径×内径×全高mm:29×25×36H)日本三博特sanplatec

15ml PFA圆底容器 (外径×内径×全高mm:29×25×36H)
产品编号: WEB15269 价格: 会员价:0元;市场价:0元 产品特点
/
产品规格

本体尺寸(mm)×外径×内径×高:29Φ×25Φ×36

瓶盖尺寸(mm)×外径×高:36Φ×40

材料

确认材料的耐药性 >> 耐药性检索

        15ml PFA圆底容器 (外径×内径×全高mm:29×25×36H)15ml PFA圆底容器 (外径×内径×全高mm:29×25×36H)产品特征

特点 :瓶子完全由PFA制造 ,耐药品性优越 。金属溶出及少 。作为无金属容 器,适合保管各种分析试料。内部形状为圆形,可以简单取出内容液。 耐热性,耐寒性优越。可在冷冻库冷冻,也可在油浴加热。可可用于广泛的温度领域(-196℃~250℃)。本品为非粘性,适合操作需要剥离或处理高粘度的样品。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

Natrix • Natrix 2 • Natrix HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Products > Crystallization Screens > Natrix • Natrix 2 > Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Applications

  • Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes

Features

  • Nucleic acid sparse matrix screen
  • Sparse matrix formulation efficiently samples salts, polyols, polymers, organics, additives & pH
  • pH range 5.6 – 8.5
  • Tube or Deep Well block format

Description

Natrix, Natrix 2 and Natrix HT are based upon published reagent formulations for the crystallization of nucleic acids and protein-nucleic acid complexes. A variety of hammerhead ribozymes and other ribozymes, RNAs, DNAs, RNA-drug complexes, and RNA-protein complexes have been crystallized using the Natrix protocols.

By using sparse matrix sampling technology, The Natrix kits allow one to quickly test wide ranges of pH, salts, and precipitants using a very small sample of nucleic acid.

Natrix screens are unique in that rather than relying solely on the traditional nucleic acid precipitant MPD, Natrix screens also utilize Polyethylene glycols (PEGs) in a variety of molecular weights (200, 400, 4,000, 8,000) as well as 2-Propanol, Polyethylene glycol monomethyl ether (PEG MME), and 1,6-Hexanediol. Many of the polymeric and low molecular weight organic precipitants are combined with various monovalent salts as precipitating agents. This combination of salts and low molecular weight organics and polyalcohols, as well as the utilization of varying chain length PEGs, has proven to be a successful combination for producing nucleic acid and protein-nucleic acid complex crystals.

Natrix contains 48 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by William Scott in 1995.

Natrix 2, an extension of Natrix, contains 48 unique reagents, 10 ml each. Natrix 2 is a biased sparse matrix screen based on extracting patterns from crystallization data as well as reagent formulations first described by Berger et al in 1996.

Natrix HT contains 1 ml of each reagent from Natrix and Natrix 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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Natrix • Natrix 2 • Natrix HT

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Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT

Natrix • Natrix 2 • Natrix HT

Click to Zoom In

Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT

CAT NO

HR2-116

NAME

Natrix

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-117

NAME

Natrix 2

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-131

NAME

Natrix HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

Natrix • Natrix 2 • Natrix HT HR2-116 Natrix DocumentsNatrix • Natrix 2 • Natrix HT HR2-116 Natrix SDSNatrix • Natrix 2 • Natrix HT HR2-117 Natrix 2 DocumentsNatrix • Natrix 2 • Natrix HT HR2-117 Natrix 2 SDSNatrix • Natrix 2 • Natrix HT HR2-131 Natrix HT DocumentsNatrix • Natrix 2 • Natrix HT HR2-131 Natrix HT SDSNatrix • Natrix 2 • Natrix HT Changes in Formulation Natrix Natrix 2 Natrix HTNatrix • Natrix 2 • Natrix HT Natrix Formulation & Scoring Data

Related Item(S)

  • Individual Natrix • Natrix 2 • Natrix HT Reagents

References

1. Scott, W. G., Finch, J.T., Grenfell, R., Fogg, J., Smith, T., Gait, M.J., Klug, A., Journal of Molecular Biology (1995) 250: 327-332.

2. Purification, crystallization and preliminary X-ray analysis of the BseCI DNA methyltransferase from Bacillus stearothermophilus in complex with its cognate DNA. E. G. Kapetaniou, D. Kotsifaki, M. Providaki, M. Rina, V. Bouriotis and M. Kokkinidis. Acta Cryst. (2007). F63, 12-14.

3. Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Forster, M. Perbandt, A. B. E. Brauer, S. Brode, J. P. Furste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.

4. Combinatorial crystallization of an RNA-protein complex. Danielle Bodrero Hoggan, Jeffrey A. Chao, G. S. Prasad, C. David Stouta and James R. Williamson. Acta Cryst (2003). D59, 466-473.

5. Cocrystallizing natural RNA with its unnatural mirror image: biochemical and preliminary X-ray diffraction analysis of a 5S rRNA A-helix racemate. Volker A. Erdmanna et al. Acta Cryst. (2007). F63, 839-843.

6. Berger, et al., A Highly Effective 24 Condition Matrix for the Crystallization of Nucleic Acid Fragments. Acta Cryst. Section D. (1996) Vol. D52 Part 3, 465-468.

7. Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Förster, M. Perbandt, A. B. E. Brauer, S. Brode, J. P. Fürste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.

8. Adams, A., Nucleic Acids Research (2002) Vol. 30, 719-725.

9. Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-b receptor promoter DNA. Agarkar et al. Acta Cryst. (2009). F65, 1261-1263.

10. Crystallization and preliminary X-ray crystallographic studies of transglutaminase 2 in complex with Ca2+. T.-H. Jang and H. H. Park. Acta Cryst. (2014). F70, 513-516 [ doi:10.1107/S2053230X1400510X ]

Natrix • Natrix 2 • Natrix HT Natrix • Natrix 2 • Natrix HT

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NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1/2×3/8(英寸))日本三博特sanplatec

NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1/2×3/8(英寸))
产品编号: WEB10825 价格: 会员价:0元;市场价:0元 产品特点
购买后可用管刀(称呼:TC-1)产品编号:W9122剪切。使用非常方便。
产品规格

PFA管 (直管 )1/2×3/8(英寸)

规格(外径×内径×厚度):12.7  ×  9.53×1.585

长度:3

材料

确认材料的耐药性 >> 耐药性检索

        NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1/2×3/8(英寸))NEW 可熔性聚四氟乙烯(PFA)直管 (称呼 : 1/2×3/8(英寸))产品特征

●注意
销售单位是3m请注意。
购买后如果需要裁切, 使用P217的管裁切刀将会非常方便。

  日本三博特sanplatec代理商 上海金畔生物科技有限公司

日本ATTO株式会社官网电泳仪电泳槽 垂直电泳仪AE-6531

日本ATTO株式会社  www.atto.co.jp

日本ATTO株式会社官网电泳仪电泳槽 垂直电泳仪AE-6531

日本ATTO株式会社  www.atto.co.jp
日本ATTOAE-3750干胶仪
日本ATTOAE-3750干胶仪
日本ATTO干胶仪AE-3711 mini
日本ATTO干胶仪AE-3711 mini
日本ATTO干胶仪AE-3750
日本ATTO干胶仪AE-3750
日本ATTO水平半干转印仪(蛋白,核酸通用型)AE-6687
日本ATTO水平半干转印仪(蛋白,核酸通用型)AE-6687
日本ATTO水平半干转印仪AE-6685P
日本ATTO水平半干转印仪AE-6685P
日本ATTO温控测序电泳槽AE-6141 Mni-Quencer
日本ATTO温控测序电泳槽AE-6141 Mni-Quencer
日本ATTO测序电泳槽AE-6155
日本ATTO测序电泳槽AE-6155询价
日本ATTO水平琼脂电泳槽AE-6111
日本ATTO水平琼脂电泳槽AE-6111
日本ATTO等电聚焦电泳仪AE-6541
日本ATTO等电聚焦电泳仪AE-6541
日本ATTO多胶垂直电泳仪AE-7341
日本ATTO多胶垂直电泳仪AE-7341
日本ATTO垂直电泳仪AE-6531
日本ATTO垂直电泳仪AE-6531
日本ATTO垂直电泳仪AE-6531
日本ATTO垂直电泳仪AE-6531
日本ATTO制备电泳AE-6750S
日本ATTO制备电泳AE-6750S
日本ATTOAE-6450垂直电泳槽
日本ATTOAE-6450垂直电泳槽

日本ATTO株式会社成立于1964年,是日本最著名的电泳仪器及生物仪器制造商,主要产品包括:各类小型电泳槽,电源,转印槽,干胶仪,胶染色仪,制备电泳,等电电泳,生物发光检测仪器。ATTO的小型电泳槽做工精致,电源精巧,进入国内两年,已深得市场厚爱。
Back in the 1960s, scholars in the field of biochemistry were hard pressed to find the types of sophisticated equipment they needed for their pioneering research. Atto Corporation was established as a specialist manufacturer in 1964 to meet their demands. The company founders had every confidence that the field of biochemistry was destined to grow. In the four decades since, Atto has invested substantial resources and money in research and development under the guidance of leading biochemists.

Since our establishment, we have continued to develop, manufacture, and sell biological macromolecules and analytical products such as chromatographs and electrophoresis apparatus. Over the last decade we have also developed and marketed a luminescence device for the research of gene expression.

Our name Atto comes from “atto,” an incredibly small unit equal to 1 x 10-18, or one-quintillionth. Modern scientists and technologists now hope to delve deeper into exploratory research on the “atto” scale. This is a field Atto is poised to enter.

In recent years, the Japanese government has begun to promote joint research between universities and enterprises. However, Atto has been engaged in collaboration with universities, and has already achieved several fruitful results to date. One of the results of collaboration won an award from the Ministry of Education, Culture, Sports, Science and Technology in 2001.

Company Profile
Founded: January, 1964
Headquarters: Tokyo, Japan
President: Shigemitsu Yamada
Business:
Development and sales of research support instruments in
biochemistry, molecular biology, and biotechnology fields
Products:
Apparatus for electrophoresis and chromatography,
imaging systems, luminometers, spectrometers, peristalic pumps
and other instruments for biological/biochemical reasearch
Clientele:
1. Universities and research institutes
2. Medical- and food-related companies
3. Overseas universities and research institute