日本SIBATA柴田科学LD-3B,LD-3C,LD-3K2,LD-5,个人暴露粉尘计LD-6N

LD-3B,LD-3C,LD-3K2,LD-5,个人暴露粉尘计LD-6N

SIBATA柴田科学
第一章,环境测定机
一,粉尘测定仪,数显粉尘计
LD-3B,LD-3C,LD-3K2,LD-5,个人暴露粉尘计LD-6N,高浓度粉尘测定仪LD-5D,
浮游粉尘浓度测定计GT-526S,804,GT-321,GT-521,GT-331,PM2.5采样器LV-250R,
碳成分定量分析仪2001A,FLD-1屋外粉尘监控器,高空广域烟雾监测系统L2S-SM2,DMA式粉尘收集装置DMA-5120,DMA-5160,DMA-5180,
AP-632T数显粉尘计检出器AP-632TL,AP-632TM,AP-632TH
粉尘数据处理系统DPS-20
空气取集分配装置ST-2
二,成套环境测定设备
室内环境测定仪IES-400
三,粉尘计iwase
烟囱用数显粉尘浓度计AP-705,烟囱用数显粉尘浓度计AP-800N
沉降粉尘计080080-011,080080-041,H型沉降粒子收集装置H,
雨水采集系统A80080-010,A80080-011,A80080-012,A80080-013
分样采集驱动装置W-2B,
干性沉降物采集装置W-2D,
分割采集装置W-2S,
大气降下物全部采集器W-102
四,口罩测试仪
口罩测试仪MTS-2,MT-03
防尘口罩性能测试装置AP-9200
口罩测试仪MT-100N,
口罩捕集效率测定装置AP-632F,
口罩过滤性能测试装置AP-6320,
面罩性能检测装置AP-9000
均一粒子发生装置AP-9000G(DOP)/AP-9000G(NaCl)
AP-9000G(PAO),PG-L
五,粉尘实验
堆积粉尘浮游再现装置SKY-2,

六,迷你空气泵
小型空气泵,MP-∑30N,MP-∑300N,MP-∑500N,MP-∑100HN,MP-∑3,低噪音小型空气吸引泵
MP-∑150S,小型通用空气泵MP-3,小型通用空气吸引泵MP-2N,MP-11T,PAS-500
手动真空泵080870-10
石鹸膜流量计BF-200,600

七,空气采样
空气泵LV-40BR,抽吸两用静音型空气泵SIP-32L,
空气泵用流量计LV-20N/LV-30N
LV-305N/LV-320N

湿式气体流量表W-NK-1A,W-NK-10A
三脚台座080160-5,PM4收集设备C30,354,空气采样器SL-30N,
多段型分粒装置C-30,滤纸架A型,滤纸架B型

空气取样机
HV-500R,HV-500R-4S,HV-500RD,HV-500RD1,HV-700R,HV-1000R
数显孔口流量计,OFD-1,
空气取样机,AN-200
HV-1000R,尘埃分粒捕集
放射性碘测定,RI-55
立式液体采集器,S-603,PM4个人取样,NWPS-254,劳作粉尘取样,PS-33,

八,
织维装粒子监控器,F-1K,纤维状粒子监测器,FS-1,
石棉纤维捕集监视器,AS-510,AS-520,大气石棉监测装置,AS-100,立式石棉纤维取样机,APS-7,APS-72,石棉个人取样,APS-1,AIP-105
试料粉碎,分析粉碎机050630-1,连续分析粉碎机050630-2
080140-61/080140-62,080140-47,025070-1(后暂缺)

小型振动器CV-1
VOC采样装置VOC-1

风速计
ISA-79,ISA-90N,ISA-911N,ISA-912N,ISA-921N,ISA-922N
风速计探头P-1,P-2,P-1H,P-2H
风速计ISA-69
寒暖计,080350-01,080350-02,080350-03
烟雾检测器,080270-01
干湿球温度指示计,WBGT-1,数显温度计SK-110TRH2 Type1,SK-110TRH2 Type3,SK-8900
SPC微型采集器G-1型SPC24,D-2型SPC24
小型数显照度计ANA-F9,数显照度计,ANA-F11,ANA-F12
疲劳测试机,RDF-1,DF-1
TPH测定器,Petro FLAG,
手动式土壤采集器,HS-25S,HS-25L,HSC-5
袖珍氯残留水质计,AQ-101,AQ-102,AQ-106,AQ-103,AQ-104,AQ-105,
残留氯测定器DPD,浊度计S-100装置,S-100本体
点触式计数器CC-1,051280-03,CL-570
BOD测定器080530-02,BOD稀释液080530-41,
氯气浓度检测机AQ-102P,POV测试品5型
POV试验纸080570-811,TBA试验纸080570-411,亚硝酸测试品080570-71,

第二章

CP-300高温マルチ,
有机合成装置,
CP-110,CP-120,CP-130,CP-140,CP-150,CP-160,CP-170,
有机合成装置本体Chemi Chemi-100
有機合成装置ケミストプラザ CPG-2000シリーズ,
CPG-2110,CPG-2120,CPP-2210,CPP-2220,DAP-10干燥空气,多检体蒸发器P12,P6,
转盘式蒸发器,R-3V,R Ⅱ-A,R2-A+P,R2-V,R2-V+P,R2-C,R2-C+P,

R-210系列,R-210A,R-210A+P,R-210V,R-210V+P,R-210C,R-210C+P,R-210E,R-210E+P,
R-210S,R-210S+P,R-210CR,R-210CR+P
R-215系列,R-215A,R-215A+P,R-215V,R-215V+P,R-215C,R-215C+P,R-215E,R-215E+P,
R-215S,R-215S+P,R-215CR,R-215CR+P
R-210A-5,R-210A-5+P-5,R-210V-5,R-210V-5+P-5,R-210C-5,R-210S-5+P-5,R-210S-5,
R-210E-5,R-210E-5+P-5,R-210CR-5,R-210CR-5+P-5,
R-215A-5,R-215A-5+P-5,R-215V-5,R-215V-5+P-5,R-215C-5,R-215C-5+P-5,R-215S-5,
R-215S-5+P-5,R-215E-5,R-215E-5+P-5,R-215CR-5,R-215CR-5+P-5

SRE-10E,防爆型轮转蒸发器RE-10A-100,R-220SE basic ,R-220SE basic D,R-220SE
大型轮转蒸发器R-250RS,R-250DS,大型轮转蒸发器R-20EU,R-20SU,R-50EU,R-50SU
R-100EU,R-100SU
蒸馏收集装置RE-20ED,RE-20SD,RE-50ED,RE-50SD,RE-100ED,RE-100SD
玻璃管干燥器GTO-2000转动加热型,GTO-2000干燥型,GTO-3000转动加热型,GTO-3000干燥型,GTO-200,B-585TO,B-585GKR
真空控制器V-850,V-855,v-801.V-802
真空控制器V-302,节水型真空泵/循环吸气机WJ-15,WJ-20
真空泵V-700ECO,V-700MECO,V-710ECO
真空系统V-701ECO,V-702,V-703,V-711ECO,V-712,V-713
低温循环装置C-331,C-307,CS-340,C-580,C-585,C-588,C-760,C-761,C-770,C-771,CC-871,低温循环水槽C-331,C-307,CS-340,C-580,C-585
低温水槽C-588P,C-588S,
低温循环水槽C-760,C-761,C-770,C-771,CC-871
小型冷却机IC-131F,IC-131C
温度调节器TC-120
冷却トラップCT-510,CT-910

恒温槽B-491,B-495,WB-23,搅拌式恒温槽WB-22S,
恒温槽TBS系列,TBS221AA,TBS271AA,TBS271DA
恒温槽TBM系列TBM106AA,TBM204AA,TBM206AA,TBM212AA
恒温槽TBN系列TBN302DA,TBN402DA,TBN602DA,TBN802DA
粘度计用恒水槽VB-3T
桌上型恒温水槽CU-110,CU-120
超音波吸量管清洗机PU-100
小型纯水制造装置PP-101,PP-201
桌上型蒸馏水制造装置WS-400,WS-800

唾液酸酶[产气英膜杆菌] exo-α-Sialidase (Clostridium perfringens) 货号:E-SIALCP Megazyme中文站

唾液酸酶[产气英膜杆菌]

英文名:exo-α-Sialidase (Clostridium perfringens)

货号:E-SIALCP

规格:5 Units

High purity recombinant exo-alpha-Sialidase (Clostridium perfringens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.18
CAZy Family: GH33

Recombinant. From Clostridium perfringens. In 3.2 M ammonium sulphate. Hydrolysis of unbranched, non-reducing terminal α-2,3-linked, α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates.

Specific activity: ~ 140 U/mg (37oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid)

Store at 4oC.

Custom quantities and formulations available up on request.

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植物激素组


产品编号 产品名称 产品规格 产品等级 产品价格
165-13831 5 Plant Growth Regulators Set A 5 
植物生长调节剂 A
1 Set 植物组织培养用

· 构成成分(各1g)

  2,4- Dichlorophenoxyacetic Acid
  Potassium 3-Indoleacetate
  Potassium 1-Naphthylacetate
  6-Benzylaminopurine
  6-Furfurylaminopurine

 

植物激素组

植物研究用试剂

乳糖/D-半乳糖[快速]检测试剂盒 Lactose/Sucrose/D-Glucose Assay Kit 货号:K-LACGAR Megazyme中文站

乳糖/D-半乳糖[快速]检测试剂盒

英文名:Lactose/Sucrose/D-Glucose Assay Kit

货号:K-LACGAR

规格:115 assays per kit

乳糖/D-半乳糖[快速]检测试剂盒

Megazyme低聚半乳糖和半乳糖检测试剂盒采用专利技术,在试剂盒中加入半乳糖变旋酶,可快速催化限速变旋步骤,室温下5分钟即可获得检测结果。试剂盒规格:115次方法:分光光度计,340nm 反应时间: 5分钟检测限: 2.96mg/L 样品类型:牛奶、乳制品(如奶油、奶粉、乳清粉、奶酪、炼乳和酸奶)、含乳食品(如保健食品、焙烤食品、婴幼儿食品、巧克力、糖果和冰淇淋)、食品添加剂、饲料、化妆品、医药及其他物料。方法认证: 通过AOAC、NBN、DIN、GOST以及德国、荷兰、瑞士和奥地利的认证.

分析物意义:常见加工食品组分,在某些情况下,精确的数值很重要,如 “无乳糖”产品 

Megazyme检测试剂盒优点:K-LACGAR试剂盒反应快(室温,5min)、试剂稳定

The Lactose/Galactose (Rapid) test kit is used for the rapid test of lactose, D-galactose and L-arabinose in food and plant products. Galactose dehydrogenase can be used the measurement and analysis of both D-galactose and L-arabinose. Suitable for the analysis of lactose in “low-lactose” or “lactose-free” samples which contain high levels of monosaccharides.

UV-method for the determination of Lactose and D-Galactose in
foodstuffs, beverages and other materials

Principle:
(β-galactosidase)
(1) Lactose + H2O → β-D-galactose + D-glucose

(galactose mutarotase)
(2) α-D-Galactose ↔ β-D-galactose

(β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+
 

Kit size: 115 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 2.96 mg/L (lactose)
Application examples:
Milk, dairy products (e.g. cream, milk / whey powder, cheese,
condensed milk and yogurt), foods containing milk (e.g. dietetic foods,
bakery products, baby food, chocolate, sweets and ice-cream), food
additives, feed, cosmetics, pharmaceuticals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, NBN,
DIN, GOST and IDF

Advantages

  • Very rapid reaction due to inclusion of galactose mutarotase (patented technology PCT / IE2004 / 00170)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Why is the borohydride reduction step required in procedure B?

Procedure B is required  for  “low-lactose” or “lactose-free” samples containing high levels of monosaccharides.  Generally, these types of samples contain high levels of  “free” galactose which causes a high background and reduces the dynamic range available to measure the galactose that is released from lactose in the test.  To avoid this the borohydride step is used to reduce the free galactose.

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. I have a high level of monosaccharides in my sample which is causing a high background level before I measure released monosaccharides. Is there a method to remove the initial monosaccharides and reduce the background level?

Instead of the normal Carrez sample treatment, 1 mL of milk is added to 4 mL of water and 1 mL of 10 mg/mL sodium borohydride (dissolved in 50 mM NaOH and less than 5 hours old).  This solution is incubated in a closed plastic container at 40˚C for 30 min, after which it is neutralised by the addition of 2.5 mL of 0.2 M acetic acid, and then simply filtered through Whatman No. 1 filter paper.  The extract, that will be hazy, is analysed without any further treatment, and 0.2 mL per assay should be used (according to the normal procedure).  Although the samples are all hazy, this haze is stable in the assay and contributes very little to the absorbance.
In the assay the borohydride reduces all reducing sugars in the milk to their sugar alcohols, i.e. glucose goes to sorbitol, galactose goes to galactitol, and the residual lactose that we are interested in goes to lactitol.  Then the usual beta-galactosidase in the kit hydrolyses the produced lactitol into galactose and sorbitol.  However, as the borohydride has been neutralised at this stage, the galactose remains as galactose, and thus can be acted upon and quantified by the galactose dehydrogenase.
The extra reagents (to K-LACGAR) that are required to perform such analyses are:

  1. Sodium borohydride (Sigma S-9125)
  2. 50 mM NaOH
  3. 0.2 M acetic acid

Note: After borohydride reduction of the sample the incubation step with beta-galactosidase should be increased to 1 hour.

Q6. Can K-LACGAR be used for the reliable detection of lactose in bakery products at a level of approximately 100 mg per 100g?

Yes, this is possible. Here are two options for sample preparation methods for pastry products:
1. Mill or homogenise sample materials.  Weigh out a representative sample and extract with water (heated to 60˚C if necessary).  Quantitatively transfer to a volumetric flask and dilute to the mark with distilled water.  Mix, filter and use the appropriately diluted, clear solution for the assay.
Alternatively the sample can be treated with Carrez reagents after the extraction with water:
2. Mill or homogenise sample materials.  Accurately weigh approx. 1 g of into a 100 mL volumetric flask, add approx. 40 mL of distilled water, mix and store at 60˚C for 15 min with occasional swirling.  Add 2 mL of Carrez II solution and mix.  Add 2 mL of Carrez I solution and mix.  Add 4 mL of 100 mM NaOH solution and mix vigorously. Dilute to volume with distilled water and mix thoroughly.  Filter an aliquot of the solution through Whatman No. 1 filter paper.
Discard the first few mL of filtrate.  Use the clear filtrate (sample solution) in the assay.  Alternatively centrifuge in a microfuge tube at 13000 x rpm and using the clear supernatant in the assay.
The procedures given here can be modified to suit the sample, e.g. the dilution effect of the lactose in the sample can be reduced by extracting the sample in a lower volume of water so that the final concentration of lactose is detectable by the kit.  This would need to be assessed by the user.

Q7. The detectable range of lactose is 0.008 – 0.16 g/L using a 1 mL sample but the detection limit is given as 0.00296 g/L for lactose? Why is the ”limit of detection” of your method different from the minimum value of the detectable range?

The linear range of 0.008 – 0.16 g/L is based on the recommended minimum absorbance change of 0.1, however some users are comfortable working below this level hence the limit of detection is based on a 1 mL sample volume and minimum absorbance change of 0.02.  The sample volume can be altered; for samples containing low concentrations of lactose the sample volume can be increased to up to 1 mL however the distilled water volume must be altered accordingly so that the final assay volume is not altered, otherwise the calculation will be affected (see page 7 of the K-LACGAR booklet).  For concentrated samples these should be diluted in distilled water and the dilution factor included into the calculation.

Q8. Is it possible to measure at a higher wavelength than 340 nm?

It is possible to measure the K-LACGAR reactions at 365 nm.  In this instance the extinction coefficient of NADH alters from 6300 [L x mol-1 x cm-1] to 3400 [L x mol-1 x cm-1] and this must be accounted for in the calculation of D-glactose and lactose.  The calculations for measurements recorded at 365 nm are shown below.
乳糖/D-半乳糖[快速]检测试剂盒 Lactose/Sucrose/D-Glucose Assay Kit 货号:K-LACGAR  Megazyme中文站
Note: Alternatively the MegaCalc application may be used for easy processing of raw data values, however if the MegaCalc application is used for calculations recorded at 365 nm then the calculated values (g/L) must be multiplied by 1.8529.

Q9. Can K-LACGAR be used to measure arabinose?

This kit can be used as described in the format below to measure L-arabinose but not D-arabinose:
FORMAT:
Wavelength:                340 nm
Cuvette:                       1 cm light path (glass or plastic)
Temperature:              ~ 25°C
Final Volume:              2.72 mL
Sample solution:         4 – 120 μg of L-arabinose per cuvette
Read against air:        without cuvette in light path
乳糖/D-半乳糖[快速]检测试剂盒 Lactose/Sucrose/D-Glucose Assay Kit 货号:K-LACGAR  Megazyme中文站
* for example with a plastic spatula or by gentle inversion after closing the cuvette with a cuvette cap or Parafilm®.
** if this “creep” rate is greater for the sample than for the blank, extrapolate the absorbances (sample and blank) back to the time of addition of suspension 5.
CALCULATION:
Determine the absorbance difference (A2-A1) for both blank and sample.  Subtract the absorbance difference of the blank from the absorbance difference of the corresponding sample, thereby obtaining DA.
The concentration of arabinose can be calculated as follows:

乳糖/D-半乳糖[快速]检测试剂盒 Lactose/Sucrose/D-Glucose Assay Kit 货号:K-LACGAR  Megazyme中文站
where:
V     = final volume [mL]
MW = molecular weight of arabinose [g/mol]
ε      = extinction coefficient of NAD+ at 340 nm
= 6300 [L x mol-1 x cm-1]
d      = light path [cm]
v      = sample volume [mL]

乳糖/D-半乳糖[快速]检测试剂盒 Lactose/Sucrose/D-Glucose Assay Kit 货号:K-LACGAR  Megazyme中文站

If the sample has been diluted in addition to the dilution during preparation, the result must also be multiplied by the additional dilution factor, F.
When analysing solid and semi-solid samples which are weighed out for sample preparation, the content (g/100 g) is calculated from the amount weighed as follows:

乳糖/D-半乳糖[快速]检测试剂盒 Lactose/Sucrose/D-Glucose Assay Kit 货号:K-LACGAR  Megazyme中文站

Q10. How do I know which procedure to use for my sample(s)?

As a general rule: Procedure A is used for samples that are known to contain low levels of free galactose. Procedure B is used for samples that are known to contain high levels of free galactose. If the free galactose content of a sample is unknown it is recommend that the galactose is measured as per the galactose assays in Procedure A.
For “lactose free” samples it is generally recommended that procedure B is used to maximise the absorbance range and enable the sensitive detection of lactose.
“Lactose free” dairy products have usually been processed whereby lactose in the original sample has been hydrolysed to glucose and galactose. These samples will contain high levels of free galactose and should be processed using procedure B. Some samples will be “lactose free” because the original sample never contained lactose so, assuming that the free galactose level is low, these samples can be processed using procedure A.

Q11. Is there a procedure to test solid samples using PROCEDURE B: (For “low-lactose” or “lactose-free” samples containing high levels of monosaccharides)?

Step 1: Add 1 g of sample (or homogenised sample) to 4 mL of water, mix then add 1 mL of 10 mg/mL sodium borohydride (dissolved in 50 mM NaOH and less than 5 hours old).  Incubate this solution in a sealed plastic container at 40°C for 30 min then neutralise by the addition of 2.5 mL of 0.2 M acetic acid.  Transfer all of the borohydride reduced sample (~ 8.5 mL) to a 10 mL volumetric flask and make the final volume to 10 mL with distilled water. Filter through Whatman No. 1 filter paper or centrifuge in a microfuge at 13000 x g and use the filtrate or supernatant directly in the assay or with an appropriate dilution in distilled water (if required).  The filtrate may be hazy but this is stable in the assay and contributes very little to the absorbance.  Typically use a sample volume of 0.2 mL in Step 2 of PROCEDURE B.
For analysis of results the dilution is 1 and the concentration of the prepared sample is 100 g/L (i.e. 1 g prepared in 10 mL).

Q12. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q13. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q14. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q15. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q16. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q17. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q18. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q19. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

Q20. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

合成·反应装置ケミスト广场CPG – 2000系列用两位阀套|柴田科学有限公司-环境检测设备、科学仪器的制造销售

产品详细

科学仪器

合成·反应装置ケミスト广场CPG – 2000系列用二人组合阀

商品代码其他情报(式样)

这个产品比较表中追加
产品照片 合成·反应装置ケミスト广场CPG - 2000系列用两位阀套|柴田科学有限公司-环境检测设备、科学仪器的制造销售
商品代码 054310 – 1807
型式
价格(不含税) 15万日元。

上海金畔生物科技有限公司

665%2F%3Fc%3D34& ” 665%2F%3Fc%3D34&

有机磷农药混合标准溶液FA-3


产品编号 产品名称 产品规格 产品等级 产品价格
156-02951 Organophosphorus Pesticide Mixture Standard Solution FA-3 (each 20μg/ml Acetone Solution)
有机磷农药混合标准溶液FA-3
1ml for Pesticide Residue Analysis

有机磷农药混合标准溶液FA-3

Organophosphorus Pesticide Mixture Standard Solution FA-3 (each 20μg/ml Acetone Solution)

成分表(共10种成分)


Omethoate

氧化乐果

Isoxathion

恶唑磷

Disulfoton(Ethylthiometon)

乙拌磷

Vamidothion

蚜灭多

Monocrotophos

久效磷

Azinphos-methyl

保棉磷

Bromophos-ethyl

乙基溴硫磷

Azinphos-ethyl

谷硫磷乙酯

Fenamiphos

苯线磷

Coumaphos

蝇毒磷

相关产品

 

产品编号

产品名

中文名

包装

152-02931

Organophosphorus   Pesticide Mixture Standard Solution FA-1 (each 20μg/ml)

有机磷农药混合标准溶液FA-1 (各20μg/ml)

1ml

159-02941

Organophosphorus   Pesticide Mixture Standard Solution FA-2 (each 20 μg/ml )

有机磷农药混合标准溶液FA-2 (各20μg/ml)

1ml

153-01481

Organophosphorus   Pesticides Mixture Standard Solution (each 1mg/ml Toluene Solution)

有机磷农药混合标准溶液(各1mg/ml甲苯溶液中)

1mL x 5

 



ICP-MS分析和ICP分析多元混合标准溶液


产品编号 产品名称 产品规格 产品等级 产品价格
134-16201 Multielement Standard Solution W-X
多元素标准溶液W-X
50mL for ICP-MS Analysis
131-16211 Multielement Standard Solution W-XI
多元混合标准溶液W-XI
50mL for ICP-MS Analysis

多元混合标准溶液W-X & W-XI

Multielement Standard SolutionW-X & W-XI

  用于ICP-MS分析和ICP分析的多元混合阳离子标准溶液;

  日本厚生劳动省根据水质标准条例制定了"电感耦合等离子体质谱仪同时分析,附录6"。此分析方法中使用的试剂包含金属离子混合标准溶液A,B,C三种混合溶液,以及镁离子标准溶液和内标混合溶液。Wako生产的多元混合标准溶液对应于此附录中的金属混合标准溶液B和内标混合溶液。

W-X   [Wako Catalog No. 134-16201 (50]



◆特点


● 更容易制备混合标准原液

● 认证检测浓度:每种元素含量100.0 ± 5.0 mg/L



产品信息


W-X134-16201), 含九种金属元素

对应于金属混合标准溶液B (100 mg/L):

镉(Cd),铬(Cr),锡(Se), 铅(Pd), 砷(As), 锌(Zn), 铝(Al), 铜(Cu), 锰(Mn)

W-XI131-16211), 含6种金属元素 (100 mg/L):

 对应内标混合溶液(100 mg/L) 钴(Co), 镓(Ga), 铟(In), 铊(Tl), 钇(Y),铍(Be) 

产品编号

产品名称

包装

所含成分

134-16201

Multielement Standard Solution W-X

多元混合阳离子标准溶液W-X

50 mL

Cd, Cr, Se, Pd, As, Zn, Al, Cu, Mn 

131-16211

Multielement Standard Solution W-XI 

多元混合阳离子标准溶液W-XI

50 mL

Co, Ga, In, Tl, Y, Be

◆相关产品

产品编号

产品名

规格

包装

138-11461

Multielement Standard Solution L-I (for ICP Analysis)
  多元素标准溶液L-1
Al:1,000 Ba:100 Ca:1,000 Cr:100 Fe:1,000 Mg:100 Pb:100 Sr:100 (mg/L in 1 mol/L·HNO3

for ICP Analysis

50 mL

135-11471

Multielement Standard Solution L-II (for ICP Analysis)
多元素标准溶液L-II
Cu:100 Mn:100 Ni:100 V:100 Zn:100 (mg/L in 1 mol/L·H2SO4)

for ICP Analysis

50 mL

137-11431

Multielement Standard Solution R-I (for ICP Analysis)
多元素标准溶液R-I
Ce:100 La:100 Pr:100 Sc:100 Y:100 (mg/L in 1 mol/L·HNO3)

for ICP Analysis

50 mL

134-11441

Multielement Standard Solution R-II (for ICP Analysis)
多元素标准溶液R-II
Eu:100 Gd:100 Nd:100 Sm:100 Tb:100 (mg/L in 1 mol/L·HNO3)

for ICP Analysis

 50 mL

131-11451

Multielement Standard Solution R-III (for ICP Analysis)
多元素标准溶液R-III
Dy:100 Er:100 Ho:100 Tm:100 Yb:100 (mg/L in 1 mol/L·HNO3)

for ICP Analysis

50 mL

139-11491

Multielement Standard Solution W-I (for ICP Analysis)
多元混合标准溶液W-I
K:2,000 Na:2,000 P:1,000 (mg/L in 1 mol/L·H2O)

for ICP Analysis

50 mL

132-11501

Multielement Standard Solution W-II (for ICP Analysis)
多元混合标准溶液W-II
Ca:1,000 Co:100 Fe:100 Mg:1,000 Mn:100 Ni:100 (mg/L in 1 mol/L·HNO3)

for ICP Analysis

50 mL

139-11511

Multielement Standard Solution W-III (for ICP Analysis)
多元混合标准溶液W-III
Cd:100 Cr:100 Cu:1,000 Pb:100 Zn:1,000 (mg/L in 1 mol/L·HNO3)

for ICP Analysis

50 mL

139-11871

Multielement Standard Solution W-IV (for ICP Analysis)
多元素标准溶液W-IV
Cd:100 Cr:100 Cu:100 Fe:100 Mn:100 Na:100 Pb:100 Zn:100 (mg/L in 0.1 mol/L · HNO3)

for ICP Analysis

50 mL

138-13781

Multielement Standard Solution W-V (for ICP Analysis)
多元混合标准溶液W-V
Al:100 B:100 Cd:100 Cr:100 Cu:100 Fe:100 Mn:100 Mo:100 Na:100 Ni:100 Pb:100 Zn:100 (mg/L in 1 mol/L·HNO3)

for ICP Analysis

50 mL

139-14551

Multielement Standard Solution W-VI (for ICP Analysis)
多元混合标准溶液 W-VI
Al:100 B:100 Cd:100 Cr:100 Cu:100 Fe:100 Mn:100   Mo:100 Na:100 Ni:100 Pb:100 Zn:100 (mg/L in 0.1 mol/L·HNO3)

for ICP Analysis

50 mL

 

标记抗PA tag抗体


产品编号 产品名称 产品规格 产品等级 产品价格
010-27721 Anti PA tag, Rat Monoclonal Antibody, Fluorescein Conjugated
抗PA tag,大鼠单克隆抗体,荧光素偶联
100 μL 免疫化学用
017-27731 Anti PA tag, Rat Monoclonal Antibody, Biotin Conjugated
抗PA tag,大鼠单克隆抗体,生物素素偶联
100 μL 免疫化学用
014-27741 Anti PA tag, Rat Monoclonal Antibody, Red Fluorochrome(635)Conjugated
抗PA tag,大鼠单克隆抗体,生物素素共轭,红色荧光色素(635)偶联
100 μL 免疫化学用

标记抗PA tag抗体

PA tag相关产品

标记抗PA tag抗体


本产品是用荧光素、生物素、红色荧光色素标记抗PA tag、大鼠单克隆抗体的抗体。可用于流式细胞分析。                                      

◆特点


● 带标记,无需二抗

● 可以用于流式细胞分析

◆产品概况


● 抗体亚类:IgG2a

● 溶液组成:含有0.05%叠氮化钠的1×PBS溶液

● 浓度:约0.5 mg/mL(以产品标签为准)

● 免疫动物:大鼠

● 克隆号:NZ-1

● 适用:流式细胞分析

● 推荐浓度:荧光素标记           1:10-1,000

             生物素标记         1:10-10,000

             红色荧光色素标记    1:10-1,000


◆应用实例


运用红色荧光色素标记抗PA tag进行抗体流式细胞仪分析

标记抗PA tag抗体


PA Tag 新型标签系统

Biopette E™多道电动移液器

品牌:美国Labnet

订货号:P3608-20-230V P3612-20-230V等

产品介绍

Biopette ETM Multichannel Electronic Pipettes 

Biopette ETM多道电动移液器是出于高效移液的设计理念,根据96孔模式,制造了8道和12道电动移液器。两种移液器都有四个量程,覆盖0.5ul1200ul范围的体积。高度精确的步进马达控制活塞,保证了它的精确性和准确性,使您的工作省时省力。轻便的重量,不管您是使用左手还是右手,您都能感觉轻松自如。

从用户的角度考虑,大型的液晶显示器和简单的操作界面,用户可以方便的在六种移液模式中转换。不需要更换吸头,您就可以在“MD”模式下迅速的移取96孔板的液体。

每支移掖器都有自校功能。移掖器的下部分可以轻松的取出进行高压灭菌。此外,每支移液器都含有移液器架、锂电池和充电器,方便使用。

n       舒适而有省力的移液操作

n       高效的96孔板操作

n       友好的操作界面,特有的6种移液模式

n       优秀的精确度和准确度

n       全自动马达驱动

  

规格

体积范围    适用吸头     增量     精确度(平均误差)  准确度(重现性)

0.5-10ul      10ul          0.1ul   +4.0 to +1.0%     <2.5 to <0.4%

2-20ul       10ul          0.1ul   +5.0 to +1.0%     <2.0 to <0.3%

10-200ul     200ul         1.0ul   +2.0 to +0.6%     <1.0 to <0.15%

100-1,200ul   1,000/1,200ul  1.0ul   +1.5 to +0.5%     <0.6 to <0.15%

注:大多数厂家生产的10ul吸头都可以适配20ul的移液器

 

订货信息

订货号                         参数

P3608-10-230V       Biopette ETM 电动移液器,0.5-10ul8道,带充电器,230V

P3608-20-230V       Biopette ETM 电动移液器,2-20ul8道,带充电器,230V

P3608-200-230V     Biopette ETM 电动移液器,10-200ul8道,带充电器,230V

P3608-1200-230V    Biopette ETM 电动移液器,100-1200ul8道,带充电器,230V

P3612-10-230V       Biopette ETM 电动移液器,0.5-10ul12道,带充电器,230V

P3612-20-230V       Biopette ETM 电动移液器,2-20ul12道,带充电器,230V

P3612-200-230V      Biopette ETM 电动移液器,10-200ul12道,带充电器,230V

P3612-1200-230V    Biopette ETM 电动移液器,100-1200ul12道,带充电器,230V

P3600-BAT             选配的锂电池,适配任何电压

P3615-CS               选配的电池装置,包括锂电池和充电器,230VEU plug

P3616-CS               选配的电池装置,包括锂电池和充电器,230VUK plug

P3617-CS               选配的电池装置,包括锂电池和充电器,230VUS plug

P3618-CS              选配的电池装置,包括锂电池和充电器,230VAU plug

P3628                    Biopette ETM 电动移液器支架(可放一支)

P3630                    Biopette ETM 电动移液器支架,聚丙烯酸材质(可放三支)

P3635                    Biopette ETM 电动移液器圆形支架(可放六支)

注:当订购230U的移液器时,注意在订货号后面加-EU,-UK,-AU

    当订购120U的移液器时,注意在订货号后面除去-230V

  • 上海金畔生物科技有限公司

    文章号:256-256

RHEODOL TW-O320V 三油酸聚氧乙烯山梨糖醇酐 聚山梨醇酯-85

RHEODOL TW-O320V

RHEODOL TW-O320V 三油酸聚氧乙烯山梨糖醇酐 聚山梨醇酯-85

规格

化学名称 三油酸聚氧乙烯山梨糖醇酐
国际化妆品成分名 聚山梨醇酯-85
外观 液体
含量 (%) 100
色度 8>(Gardner)
熔点(℃) -20
酸值 2>
皂化值 83-93
羟值 39-52
亲水亲油平衡 11
溶解度(10%Liq.、25℃) ・水:凝胶化, ・酒精:易溶, ・正己烷:易溶, ・二甲苯:溶解性低
SP Value〔(Mpa)1/2 18.41(Hoy method)
应用 药品和化妆品中的乳化剂,乳化、聚合反应中的稳定剂,有色材料中的稳定剂,农用化学品助剂中的乳化剂。
特性 亲水性的乳化剂和分散剂对于芳香类的化学品和油脂类有增溶作用。优良的亲水乳化剂如、、系列能和嗜油乳化剂结合产生良好的水油平衡性。
生产地区 亚洲
包装 18千克/罐,200千克/桶

日本JaICA Test kit for Potential Anti Oxidant (PAO)

日本JaICA Test kit for Potential Anti Oxidant (PAO)
日本老化制御研究所 Jaica代理
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
Suitable for detection of total antioxidant capacity in serum and food extracts. For research use only.
Antioxidant assay:

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.
Principle of this assay:

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.
Specifications:

Method: Colormetric assay(detection: 480 – 492 nm)
Assay range: 21.9 – 4378 碌mol/L (cupric ion reducing power)
Format: 96 wells
Storage: Room temperature (10 – 25掳C)
Applications: Human and animal serum samples, foods and beverage samples.
Required but not provided: A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).

 
Content of this kit: 试剂盒组成

Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++ solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate (96 wells)

Assay procedure:

1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 碌L of Sample Diluent, and add 10 碌L of standards or diluted samples.
3) Pour 200 碌L of mixture to Micro titer plate. Use 200 碌L of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 碌L of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 碌L of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 – READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (碌mol/L).
1mM of uric acid = 2189 碌mol/L (copper reducing power)

References

1) Oxidative imbalance and cathepsin D changes as peripheral blood biomarkers of Alzheimer disease: A pilot study
E Strafacea, P Matarresea, L Gambardella, R Vona, A Sgadari,MC Silveri, W Malorni
FEBS Letters 579, p2759-766 (2005)
2) Oxidative stress and its association with coronary artery disease and different atherogenic risk factors
C. VASSALLE, L. PETROZZI , N. BOTTO, M. G. ANDREASSI and G. C. ZUCCHELLI
Journal of Internal Medicine 256, p308-315(2004)
3) Antioxidant capacity as a reliable marker of stress in dairy calves transported by road
P Pregel, E Bollo, FT Cannizzo, B Biolatti, E Contato, and PG Biolatti
Veterinary Record 156, p53-54 (2005)
4) Vitamin E-coated dialyzers reduce oxidative stress related proteins and markers in hemodialysis ? a molecular biological approach.
LA Calo, A Naso, E Pagnin, PA Davis, M Castoro, R Corradin, P Riegler, C Cascone, W Huber and A Piccoli
Clinical Nephrology, Vol.62(5), p355-361 (2004)
5) Oxidative stress-related factors in Bartter’s and Gitelman9s syndrome: relevance for angiotensin IIsignalling.
Calo LA, Pagnin E, Davis PA, Sartori M, Semplicini A.
Nephrol Dial Transplant 18(8) p1518-1525 (2003)
6) Effect of epoetin on HO-1 mRNA level and plasma antioxidants in hemodialysis patients.
Calo LA, Stanic L, Davis PA, Pagnin E, Munaretto G, Fusaro M, Landini S, Semplicini A, Piccoli A.
Int. J Clin. Ther 41(5), p187-192 (2003)
7) Restored Antioxidant Capacity Parallels the Immunologic and Virologic Improvement in Children with Perinatal Human Immunodeficiency Virus Infection Receiving Highly Active Antiretroviral Therapy.
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Product name Code Assay range Assay time Format
Test kit for Potential Anti Oxidant (PAO) KPA-050 21.9-4378 碌mol/L 5 minutes 96 wells

日本JaICA Test kit for Potential Anti Oxidant (PAO)   说明书  技术资料

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