消化性溃疡药成分


产品编号 产品名称 产品规格 产品等级 产品价格
185-01564 Ranitidine Hydrochloride 盐酸雷尼替丁组胺 500mg 生物化学用
181-01561 Ranitidine Hydrochloride 盐酸雷尼替丁组胺 5g 生物化学用
187-01563 Ranitidine Hydrochloride 盐酸雷尼替丁组胺 25g 生物化学用
067-03931 Famotidine 法莫替丁 5g 生物化学用
065-03932 Famotidine 法莫替丁 25g 生物化学用
147-08541 Nizatidine 尼扎替丁 5g 药理研究用
145-08542 Nizatidine 尼扎替丁 25g 药理研究用
036-16311 Cimetidine 甲氰咪胍 5g 生物化学用
034-16312 Cimetidine 甲氰咪胍 25g 生物化学用
123-05861 Lansoprazole 兰索拉唑 250mg 药理研究用
129-05863 Lansoprazole 兰索拉唑 1g 药理研究用
150-02091 Omeprazole 奥美拉挫 10g 生物化学用
158-02092 Omeprazole 奥美拉挫 25g 生物化学用
190-12061 (±)-Sulpiride 舒必利 1g 生物化学用
198-12062 (±)-Sulpiride 舒必利 25g 生物化学用
196-12063 (±)-Sulpiride 舒必利 100g 生物化学用
169-17251 Pirenzepine Dihydrochloride Monohydrate 盐酸哌仑西平一水合物 1g 生物化学用
320-20571 Guaiazulene 愈创蓝油烃 10g
328-20572 Guaiazulene 愈创蓝油烃 25g
329-26462 5-Aminosalicylic Acid 5-氨基水杨酸 25g
323-26465 5-Aminosalicylic Acid 5-氨基水杨酸 500g
019-21211 Aldioxa 尿囊素铝 5g 药理研究用
017-21212 Aldioxa 尿囊素铝 25g 药理研究用
098-04581 Irsogladine Maleate 马来酸依索拉定 100mg 生物化学用
094-04583 Irsogladine Maleate 马来酸依索拉定 500mg 生物化学用
206-15731 Teprenone(mixture of isomers) 替普瑞酮(同分异构体混合物) 1g 生物化学用
202-15733 Teprenone(mixture of isomers) 替普瑞酮(同分异构体混合物) 5g 生物化学用
194-13321 Sodium Alginate 80-120 海藻酸钠 100g
196-13325 Sodium Alginate 80-120 海藻酸钠 500g
195-14831 Sofalcone 索法酮 5g 药理研究用
193-14832 Sofalcone 索法酮 25g 药理研究用

消化性溃疡药成分


◆组织胺H2受体阻断药成分

盐酸雷尼替丁组胺(Ranitidine   Hydrochloride)


CAS No. 66357-59-3

C13H22N4O3S・HCl=350.86

纯度:97.0+%(Titration)

可溶性溶剂:水

用途(作用):组织胺H2受体阻断剂。阻断胃粘膜的组织胺H2受体,抑制胃酸分泌。H2的阻断作用是甲氰咪胍的10倍以上,对胃蛋白酶的分泌也有抑制作用。


消化性溃疡药成分

法莫替丁(Famotidine)


CAS No. 76824-35-6

C8H15N7O2S3=337.45

纯度:98.0+%(Titration)

可溶性溶剂:稀盐酸

用途(作用):组织胺H2受体阻断剂。阻断胃粘膜的组织胺H2受体,抑制胃酸分泌。


消化性溃疡药成分

尼扎替丁(Nizatidine)


CAS No. 76963-41-2

C12H21N5O2S2=331.46

纯度:98.0+%(HPLC)

可溶性溶剂:甲醇

保存条件:冷藏

用途(作用):组织胺H2受体阻断剂。阻断胃粘膜的组织胺H2受体,抑制胃酸分泌。


消化性溃疡药成分

甲氰咪胍(Cimetidine)


CAS No. 51481-61-9

C10H16N6S=252.34

纯度:98.0+%(HPLC)

可溶性溶剂:甲醇

用途(作用):阻断组织胺H2受体。另外也有抗雄性激素作用。


消化性溃疡药成分

◆原子泵抑制剂成分


兰索拉唑(Lansoprazole)


CAS No. 103577-45-3

C16H14F3N3O2S=369.36

纯度:97.0+%(HPLC)

可溶性溶剂:N,N-二甲基甲酰胺

用途(作用):原子泵抑制剂。移动至壁细胞,抑制K+-ATPase活性从而抑制胃酸分泌。


消化性溃疡药成分

 

 

奥美拉挫(Omeprazole)

CAS No. 73590-58-6

C17H19N3O3S=345.42

纯度:98.0+%(HPLC)

可溶性溶剂:二氯甲烷

用途(作用):原子泵抑制剂。移动至壁细胞,抑制K+-ATPase活性从而抑制胃酸分泌。


消化性溃疡药成分

其他消化性溃疡药成分


舒必利((±)-Sulpiride)


CAS No. 15676-16-1

C15H23N3O4S=341.43

纯度:98.0+%(Titration)

可溶性溶剂:稀醋酸

用途(作用):多巴胺D2 受体阻断剂。通过特异性阻断多巴胺D2受体使乙酰胆碱游离。


消化性溃疡药成分

盐酸哌仑西平一水合物(Pirenzepine   Dihydrochloride Monohydrate)


CAS No. 28797-61-7

C19H21N5O2・2HCl・H2O=442.34

纯度:98.0+%(Titration)(无水物换算)

可溶性溶剂:水

用途(作用):抗胆碱作用剂。对蝇蕈碱M1受体有阻断作用。


消化性溃疡药成分

愈创蓝油烃(Guaiazulene)


CAS No. 489-84-9

C15H18=198.31

纯度:96.0+%

可溶性溶剂:硫酸、磷酸

用途(作用):有抗炎症作用。


消化性溃疡药成分

5-氨基水杨酸(5-Aminosalicylic   Acid)


CAS No. 89-57-6

C7H7NO3=153.14

纯度:97.0+%(HPLC)

用途(作用):磺胺类药。通过肠内细菌将偶氮基还原性裂开,有抗炎症作用。

消化性溃疡药成分

尿囊素铝(Aldioxa)


CAS No. 5579-81-7

C4H7AlN4O5=218.10

保存条件:冷藏

用途(作用):抗胃蛋白酶化合物。可抑制胃蛋白酶活性。

消化性溃疡药成分

马来酸依索拉定(Irsogladine Maleate)


CAS No. 84504-69-8

C9H7Cl2N5・C4H4O4=372.16

纯度:97.0+%(Titration)

可溶性溶剂:甲醇

用途(作用):增加胃粘膜的cAMP量。

消化性溃疡药成分

替普瑞酮(同分异构体混合物)(Teprenone(mixture of isomers))


CAS No. 6809-52-5

C23H38O=330.55

纯度:97.0+%(HPLC)

用途(作用):促进生化前列腺素(PGE2、PGI2)的生物合成。

消化性溃疡药成分



海藻酸钠(Sodium   Alginate 80-120)


CAS No. 9005-38-3

可溶性溶剂:水(会成为粘稠的糊状液体)

用途(作用):有保护胃粘膜作用。

索法酮(Sofalcone)


CAS No. 64506-49-6

C27H30O6=450.52

纯度:97.0+%

可溶性溶剂:甲醇

保存条件:冷藏

用途(作用):有增加生化前列腺素分泌的作用。

消化性溃疡药成分

相关资料详情请查看:http:///pdf/show/80.html



EV-Save™ 细胞外囊泡封闭试剂


产品编号 产品名称 产品规格 产品等级 产品价格

抑制细胞外囊泡对实验耗材的吸附EV-Save™ 细胞外囊泡封闭试剂

EV-Save™ 细胞外囊泡封闭试剂

 


  本品是抑制细胞培养上清中的细胞外囊泡吸附于样本管或移液枪头等实验耗材的聚合物试剂。能防止实验和保存时细胞外囊泡吸附所引起的损耗,提高回收率。本品可在细胞外囊泡(含外泌体)的超滤、提取和保存前使用。

  使用MagCapture™ Exosome Isolation Kit PS(产品编号299-77603, 293-77601)从细胞培养上清中提取外泌体时,搭配本品效果更佳。

 


◆使用前提示


● 在血清、血浆或杂质较多的样本中,本品的使用效果会受到影响。

● 本品含有聚合物。若担心聚合物会影响后续实验的结果,请不要使用本品。

 


◆特点


● 抑制培养上清中或提取后细胞外囊泡对实验耗材的吸附

● 用法简单,只需往样本中添加本品即可

 


◆EV-Save™ 的吸附抑制效果~样本管~


下面来验证一下,使用EV-Save™ 是否能抑制提取外囊泡对试管的吸附。

 


■实验条件


  用MagCapture™ Exosome Isolation Kit PS(产品编号:293-77601)从COLO201细胞的培养上清中提取出外泌体。

  将3 ng/µL外泌体放入试管中静置3分钟。然后用移液器转移到其他样本管,重复本步骤4次以上。

  在含EV-Save™ 的实验组中,将EV-Save™ 按照1/100体积添加到样本中,然后进行移液。

  使用PS Capture™ 外泌体ELISA试剂盒(抗小鼠IgG POD)(产品编号:297-79201)对外泌体的CD63信号进行定量,验证移液次数与外泌体减少比率的关系。

  将移液前的CD63信号设定为100%,相对值见图表。


EV-Save™ 细胞外囊泡封闭试剂


【结果】

在普通样本管和低蛋白吸附样本管中,移液操作都会引起外泌体减少。而添加EV-Save™ 几乎能完全抑制移液引起的外泌体损耗。

 


◆EV-Save™ 的吸附抑制效果~同时使用MagCapture™ Exosome Isolation Kit PS~


下面,在利用MagCapture™ Exosome Isolation Kit PS从TIG3细胞培养上清中提取外泌体的过程中,验证添加EV-Save™ 是否能抑制外泌体的损耗。

 


■实验条件


  使用MagCapture™ Exosome Isolation Kit PS,分别从1mL COLO201、TIG3和人iPS细胞的培养上清中提取外泌体,培养上清没有经过超滤浓缩。

  提取外泌体时分别进行了以下两项操作,"往各个培养上清中添加/不添加EV-Save™","往MagCapture™ Exosome Isolation Kit PS附带的'Exosome Elution Buffer'中添加/不添加EV-Save™"。

  使用PS Capture™ 外泌体ELISA试剂盒(抗小鼠IgG POD)(产品编号: 297-79201)对外泌体的CD63信号进行定量,并将其信号强度作为反应为外泌体量的标记。

  假设移液前的CD63信号为100%,相对值见图表。


EV-Save™ 细胞外囊泡封闭试剂


-/-: 培养上清没有添加EV-Save™/ "Exosome Elution Buffer"没有添加EV-Save™

-/+: 培养上清没有添加EV-Save™/ "Exosome Elution Buffer"添加了EV-Save™

+/+: 培养上清添加了EV-Save™/ "Exosome Elution Buffer"添加了EV-Save™

 

【结果】

添加了EV-Save™ 的TIG3的细胞培养上清及MagCapture™ Exosome Isolation Kit PS附带的"Exosome Elution Buffer"实验组,比两者都没添加的实验组,外泌体的回收量增加了大约20%。

 


◆超滤时EV-Save™的吸附抑制效果~人iPS细胞~


接下来验证人iPS细胞培养上清在进行超滤(Vivaspin 20,100K)浓缩前后,是否有发生外泌体损耗。

EV-Save™ 细胞外囊泡封闭试剂


超滤后,培养上清中的外泌体会损耗大约60%,但添加EV-Save™ 后,几乎能完全抑制超滤引起的外泌体损耗。

 


◆EV-Save™ 对细胞的毒性评估~人iPS细胞~


接着,使用人iPS细胞检验EV-Save™ 的细胞毒性。将添加了1/100 EV-Save™ 的达到1/5培养基量的TBS溶液加入培养基。人iPS细胞在配制好的培养基中培养3日,然后测量活细细胞数。

EV-Save™ 细胞外囊泡封闭试剂


EV-Save™ 几乎不影响人iPS细胞的增殖。

 


◆使用方法


使用MagCapture™ Exosome Isolation Kit PS(产品编号299-77603,293-77601)提取细胞培养上清中的细胞外囊泡


1.将EV-Save™ 细胞外囊泡封闭试剂添加到细胞培养上清中,100倍稀释于细胞培养上清,上下颠倒或轻敲使其混合。

※细胞培养上清需要超滤浓缩时,请在浓缩前添加本品。

2.按照MagCapture™ Exosome Isolation Kit PS的说明书进行实验,直到"洗脱细胞外囊泡"这一步。

3.将EV-Save™ 细胞外囊泡封闭试剂添加到MagCapture™ Exosome Isolation Kit PS附带的"Exosome Elution Buffer"中,100倍稀释于"Exosome Elution Buffer",颠倒或轻敲使其混合。

4.按照MagCapture™ Exosome Isolation Kit PS说明书洗脱细胞外囊泡。

5.保存洗脱后的细胞外囊泡,或者进行后续实验。

 


使用其他方法从细胞培养上清中提取细胞外囊泡


1. 将EV-Save™ 细胞外囊泡封闭试剂添加到细胞培养上清中,100倍稀释于细胞培养上清,颠倒或轻敲使其混合。

2. 将EV-Save™ 细胞外囊泡封闭试剂添加到所提取的细胞外囊泡中,100倍稀释于细胞外囊泡储存液,颠倒或轻敲使其混合。

3. 保存洗脱后的细胞外囊泡,或者进行后续实验。


 

◆产品列表


产品编号

产品名称

包装

058-09261

EV-Save™ Extracellular Vesicle Blocking Reagent
EV-Save™ 细胞外囊泡吸附抑制剂

1 mL



◆相关产品


■试剂盒产品


产品编号

产品名称

包装

299-77603

MagCapture™ Exosome Isolation Kit PS
MagCapture™ 外泌体提取试剂盒PS

2次

293-77601

10次

290-35591

Magnet Stand
MagCapture™ 系列磁珠捕获用磁力架

1个

297-79201

PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD)
PS Capture™ 外泌体ELISA试剂盒(抗小鼠IgG POD)

96次

297-79701

PS Capture™ Exosome Flow Cytometry Kit
PS Capture™外泌体流式试剂盒

300次

290-80301

PS Capture™ Exosome Isolation Resin Kit

1试剂盒:0.5mL Slurry



■抗体


产品编号

产品名称

交叉性

包装

016-27061

Anti CD63, Monoclonal Antibody (3-13)
抗CD63单抗(3-13)

20 µL

012-27063

100 µL

018-27641

Anti CD63, Monoclonal Antibody (3-13), Fluorescein Conjugated
抗CD63,单克隆抗体(3-13),荧光素结合

25次

014-27643

100次

欲了解更多相关产品信息,请点击文字:

MagCapture™ 外泌体提取试剂盒

EV-Save™ 细胞外囊泡封闭试剂

抑制对细胞外囊泡的实验仪器的吸附

脂溶性维生素


产品编号 产品名称 产品规格 产品等级 产品价格
039-00291 Calciferol 钙化醇 1g
224-00361 Cholecalciferol 胆钙化醇 100mg
220-00363 Cholecalciferol 胆钙化醇 1g
201-18483 (±)-α-Tocopherol Acetate (±)-α-生育酚乙酸酯 5g
203-18482 (±)-α-Tocopherol Acetate (±)-α-生育酚乙酸酯 25g
205-18481 (±)-α-Tocopherol Acetate (±)-α-生育酚乙酸酯 100g
221-00371 Vitamin K1 维生素K1 1g
227-00373 Vitamin K1 维生素K1 5g
229-00372 Vitamin K1 维生素K1 25g
035-03831 Cocarboxylase 辅羧酶 100mg
031-03833 Cocarboxylase 辅羧酶 1g
033-03832 Cocarboxylase 辅羧酶 25g
068-00161 Flavin Adenine Dinucleotide Disodium Salt n-Hydrate 黄素腺嘌呤二核苷酸磷酸钠盐n水合物 10mg
064-00163 Flavin Adenine Dinucleotide Disodium Salt n-Hydrate 黄素腺嘌呤二核苷酸磷酸钠盐n水合物 100mg
062-00164 Flavin Adenine Dinucleotide Disodium Salt n-Hydrate 黄素腺嘌呤二核苷酸磷酸钠盐n水合物 1g

脂溶性维生素


◆维生素D2

钙化醇(Calciferol)

CAS No. 50-14-6

C28H44O=396.66

可溶性溶剂:乙醇

用途(作用):维生素D2化合物。

脂溶性维生素

◆维生素D3

胆钙化醇(Cholecalciferol)

CAS No. 67-97-0

C27H44O=384.64

纯度:97.0+%(HPLC)

可溶性溶剂:乙醇

用途(作用):维生素D3化合物。

脂溶性维生素

◆维生素E

(±)-α-生育酚乙酸酯((±)-α-Tocopherol Acetate)

CAS No. 7695-91-2

C31H52O3=472.74

纯度:96.0+%(HPLC)

可溶性溶剂:丙酮

用途(作用):维生素E化合物。

脂溶性维生素



◆维生素K1

维生素K1(Vitamin K1)

CAS No. 84-80-0

C31H46O2=450.70

纯度:97.0 ~ 102.0%(Absorptiometry)

可溶性溶剂:2,2,4- 三甲基戊烷

用途(作用):维生素K1化合物。

脂溶性维生素



◆其他维生素相关成分

辅羧酶(Cocarboxylase)

CAS No. 154-87-0

C12H19ClN4O7P2S=460.77

纯度:90.0+%(HPLC)

可溶性溶剂:水

用途(作用):硫胺素'(维生素B1)的二磷酸(焦磷酸)酯化合物。参与羧基酶进行的脱碳酸反应。

脂溶性维生素



黄素腺嘌呤二核苷酸磷酸钠盐n水合物(Flavin Adenine   Dinucleotide Disodium Salt   n-Hydrate)

C27H31N9Na2O15P2・nH2O

(C27H31N9Na2O15P2=829.51)

纯度:98.0 ~ 102.0+%(Absorptiometry)

(无水物换算)

可溶性溶剂:水

用途(作用):作为黄素酶的辅酶参与细胞内的氧化还原系反应和线粒体上的电子传递反应、糖类、脂质、蛋白质等活体代谢反应。

脂溶性维生素

相关资料详情请查看:http:///pdf/show/80.html



琥珀酸/丁二酸检测试剂盒 Succinic Acid Assay Kit 货号:K-SUCC Megazyme中文站

琥珀酸/丁二酸检测试剂盒

英文名:Succinic Acid Assay Kit

货号:K-SUCC

规格:20 assays (manual) / 200 assays

The Succinic Acid test kit is suitable for the specific assay of succinic acid in wine, cheese, eggs, sauce and other food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Succinic Acid in foodstuffs,
feed, wine and other materials

Principle:
(succinyl-CoA synthetase)
(1) Succinic acid + ATP + CoA → ADP + succinyl-CoA + Pi

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → NAD+ + L-lactic acid

Kit size: 20 assays (manual) / 200 (microplate)
/ 270 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.26 mg/L
Application examples:
Wine, fruit and vegetables, soy sauce, cheese, egg, egg products
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by EEC

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction (even at room temperature)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

Q1. Is the Succinic Acid Assay Kit (K-SUCC) suitable for measurement using cell culture media samples?

Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit.  It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed by dilution (if required) in distilled water. 

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

 

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q6. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q7. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the 
K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch 
this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q8. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q9. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q10. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q11. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q12. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q14. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

视频

DryKeeper 自动C型干燥箱 C-3BS 遮光(电解法原理)


产品编号 产品名称 产品规格 产品等级 产品价格
WEB0003NEW DryKeeper 自动C型干燥箱 C-3BS 遮光(电解法原理) 1 set
WEB0003 DryKeeper 自动C型干燥箱 C-3BS 遮光(干燥剂除湿) 1 set
WEB0001 DryKeeper 自动C型干燥箱 C-3B 黑框(电解法原理) 1 set
WEB0002 DryKeeper 自动C型干燥箱 C-3W 白框(电解法原理) 1 set
WEB0004 备用隔板(C-3用) 1 pc

DryKeeper 自动C型干燥箱C-3BS 遮光(电解法原理)-产品编号:WEB0003NEW

采用高分子电解质膜的水分子电解法,柜内湿度保持在30~40%RH的低湿度范围。

LED灯,低功耗

BS :黑框,本体茶色玻璃


DryKeeper 自动C型干燥箱 C-3BS 遮光(电解法原理)


产品编号

产品名称

规格

WEB0003现货

DryKeeper 自动C型干燥箱 C-3BS 遮光(干燥剂除湿法)

1 set

WEB0003NEW

DryKeeper 自动C型干燥箱 C-3BS 遮光(电解法原理)

1 set

※有效的内部尺寸(mm):255W×338D×452H 

※外部尺寸(mm):扣锁款 317W×392D×617H

※重量:约7㎏

注意:两款干燥箱的除湿原理不同。

◆除湿原理

采用高分子电解质膜的水分子电解法,通过电解箱内水分进行随时除湿。


◆特色


● 全自动式,无需更换硅胶。常温下柜内湿度可保持在30~40%RH,适合长时间保管物品。

● 结合模塑部件,功能和强度都得到进一步改善。

● 柜内采用空气循环对流风扇,除湿能力大幅提高。

● 隔板位置可根据保管物品的大小进行调整。(15格,每格28mm)


◆相关产品

WEB0001 DryKeeper 自动C型干燥箱C-3B 黑框(电解法原理)

WEB0002 DryKeeper 自动C型干燥箱C-3W 白框(电解法原理)

WEB0003 DryKeeper 自动C型干燥箱C-3BS 遮光(干燥剂除湿法)

抗躁狂药


产品编号 产品名称 产品规格 产品等级 产品价格
122-01132 Lithium Carbonate 碳酸锂 25g
124-01131 Lithium Carbonate 碳酸锂 100g
126-01135 Lithium Carbonate 碳酸锂 250g

抗躁狂药


◆碳酸锂(Lithium Carbonate)


CAS No. 554-13-2

Li2CO3=73.89

纯度:99.0+%(Titration)(干燥后)

可溶性溶剂:稀盐酸

用途(作用):作用于多数中枢神经系统的神经传递物质和受体。

Li2CO3


相关资料详情请查看:http:///pdf/show/80.html



α-(1-2,3,4,6)-L-岩藻糖苷酶(智人) α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens) 货号:E-FUCHS Megazyme中文站

α-(1-2,3,4,6)-L-岩藻糖苷酶(智人)

英文名:α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens)

货号:E-FUCHS

规格:10 Units at 25°C (~ 34 U at 37°C)

High purity α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.51
CAZy Family: GH29
CAS: 9037-65-4

alpha-L-fucosidase; alpha-L-fucoside fucohydrolase

Recombinant. From Homo sapiens.
In 3.2 M ammonium sulphate.

Specific activity: 7 U/mg protein (on pNP-α-L-fucopyranoside) at pH 4.0 and 25oC; ~ 23 U/mg at 37oC; ~ 68 U/mg at 50oC.

Stability: > 2 years 4oC.

 

暂无问题解答

暂无视频

兽药混合标准液(喹诺酮类药物)


产品编号 产品名称 产品规格 产品等级 产品价格
227-02051 Veterinary Drug Mixture Standard Solution (Quinolone) (each 20μg/ml)
 兽药混合标准液(喹诺酮类)(各20μg/ml)
1ml for High Performance Liquid Chromatography
223-02053 Veterinary Drug Mixture Standard Solution (Quinolone) (each 20μg/ml)
 兽药混合标准溶液(喹诺酮类)(各20μg/ml)
1mlx5A for High Performance Liquid Chromatography

兽药混合标准液(喹诺酮类药物)(各20μg/ml)

Veterinary Drug Mixture Standard Solution (Quinolone) (each 20μg/ml)

 

 

成分


马波沙星、诺氟沙星、氧氟沙星、环丙沙星(盐酸盐水合物)、达氟沙星、恩氟沙星、奥比沙星、盐酸沙氟沙星、二氟沙星、恶喹酸、萘啶酸、氟甲喹、吡咯酸

 

每个成分浓度为:20μg/ml;


 

相关产品

产品编号

产品名

中文名

包装

220-02203

Veterinary   Drug Mixture Standard Solution (Hormone) (each 20μg/ml Acetonitrile Sol

兽药混合标准液(激素类)(各20μg/ml乙腈溶液)

1ml

224-02201

1ml x5A

225-02091

Veterinary   Drug Mixture Standard Solution (Macrolide) (each 20μg/ml Acetonitrile Sol

兽药混合标准液(大环内酯类)(各20μg/ml乙腈溶液)

1ml

221-02093

1ml x5A

224-02083

Veterinary   Drug Mixture Standard Solution (Sulfonamide+Antifolate) (each 20μg/ml)

兽药混合标准溶液(磺胺类+抗叶酸制剂类)(各20μg/ml)

1ml x5A

228-02081

1ml

224-02103

Veterinary   Drug Mixture Standard Solution (Dye) (each 20μg/ml   Methanol Solution)

兽药混合标准溶液(染料类)(各20μg/ml)

1ml x5A

228-02101

1ml

220-01681

Veterinary   Drug Mixture Standard Solution PL-1-3 (each 20μg/ml   Methanol Solution)

兽药混合标准液PL-1-3(各20μg/ml甲醇溶液)

1ml x 5A

226-01683

1ml

223-01693

Veterinary   Drug Mixture Standard Solution PL-2-1 (each 20μg/ml   Methanol Solution)

兽药混合标准液PL-2-1(各20μg/ml甲醇溶液)

1ml

227-01691

1ml x5A

卡尔费休试剂-库伦法/容量法试剂


产品编号 产品名称 产品规格 产品等级 产品价格
AQ-AXI AQUAMICRON® AXI (621-01755)
 可代替全部库伦试剂,无吡啶AXI
500mL

卡尔费休试剂-库伦法/容量法试剂AQUAMICRON® 卡尔费休试剂



卡尔费休试剂-库伦法/容量法试剂

卡尔费休法使用卡尔费休试剂测定水分含量,该试剂定量地、有选择性地与水发生反应。卡尔费休试剂的组成成分是碘、二氧化硫、盐基和溶剂,如酒精。

这种方法可用于库伦及容量滴定系统。该法基于卡尔费休试剂与水反应的碘定量法,如果试样中含有能够与碘反应的物质或者某些通过碘化物氧化而产生碘的物质,结果出现误差。

即使某些物质不能直接滴定,其水分还可以用间接法加以测定,如水汽化法。详情请向金畔()垂询。

库伦试剂(AQUAMICRON® )特别:AXI可代替AX,价格更优。


货号

品名

规格

包装

主要溶剂

用途

621-01755

AQUAMICRON® AXI (AQ-AXI)

可代替全部库伦试剂,无吡啶AXI

最大水分0.15 mg H2O/mL

500 mL

甲醇,碳酸丙烯酯,不含氯仿和吡啶

阳极液:检测有机溶剂、无机物化学品、油类、石油产品、各种汽油等

604-07485

AQUAMICRON® AX

阳极液,用于一般用途AX

601-07255

AQUAMICRON® AS

阳极液,用于油类AS

最大水分0.15 mg H2O/mL

500 mL

甲醇,氯仿,含有4-二甲胺基吡啶

阳极液:检测石脑油、汽油、柴油、绝缘油等

605-07591

AQUAMICRON® AKX

阳极液,用于酮类AKX

最大水分0.15 mg H2O/mL

500 mL

碳酸丙烯酯,二甘醇-甲醚,不含氯仿,含有4-二甲胺基吡啶

阳极液:检测酮类、硅油、低羟基酸、某些醛类(芳香族化合物)等

602-07501

AQUAMICRON® CXU

阴极液,通用CXU

最大水分0.6 mg H2O/mL

5 m10

甲醇,不含吡啶

阴极液:与AXI/AX/AS/AKX配合使用

632-07065

AQUAMICRON® FLS

用于无玻璃型电解池FLS

最大水分0.15 mg H2O/mL

500 mL

甲醇,碳酸丙烯酯,不含吡啶

适用于一液型、无玻璃型电解池:有机溶液(酒精、酯类、苯、甲苯),无机气体等

 


◆容量滴定法试剂:SS-Z(无吡啶型)


类别

货号

品名

包装

用途

规格

滴定剂

631-03495

AQUAMICRON Titrant SS-Z 1mg (Karl Fischer Reagent SS-Z)

容量法,一般用途,滴定剂SS-Z 1mg,无吡啶

500 mL

适合于一般用途低含水量,溶剂是二甘醇-甲醚

滴定量0.71.2 mg H2O/mL

634-03505

AQUAMICRON Titrant SS-Z 3mg (Karl Fischer Reagent SS-Z)

容量法,一般用途,滴定剂SS-Z 3mg,无吡啶

500 mL

适合于一般用途,溶剂是二甘醇-甲醚

滴定量2.53.5 mg H2O/mL

631-03515

AQUAMICRON Titrant SS-Z 5mg (Karl Fischer Reagent SS-Z)

容量法,一般用途,滴定剂SS-Z 5mg,无吡啶

500 mL/1 L

一般用途高含水量,溶剂是二甘醇-甲醚

滴定量4.55.5 mg H2O/mL

636-03521

脱水溶剂

608-07525

AQUAMICRON Solvent GEX

容量法溶剂,一般用途,无吡啶GEX溶剂

500 mL

【通用】有机溶剂、无机化学品、农用化学品,医药品,化肥,清洁剂,食品等

含水量0.2 mg H2O/mL

635-03535

AQUAMICRON Solvent OLX

容量法溶剂,用于油类,无吡啶OLX溶剂

500 mL

【油类用】石脑油,汽油,柴油,绝缘油等

含水量0.5 mg H2O/mL

605-07535

AQUAMICRON Solvent OLII

容量法溶剂,用于油类脂肪,无吡啶OLII溶剂

500 mL

【油类.油脂类用】石脑油,汽油,柴油,重油,绝缘油,油类和脂肪(硬化油,人造黄油等)等

含水量0.3 mg H2O/mL

632-03545

AQUAMICRON Solvent KTX

容量法溶剂,用于酮类,无吡啶KTX溶剂

500 mL

【酮类用】酮类,硅油,乙酸和其他低羟基酸,醛类(乙醛除外)等

含水量0.5 mg H2O/mL

603-07335

AQUAMICRON Solvent SU

容量法溶剂,用于糖类,无吡啶SU溶剂

500 mL

【糖类用】糖类,蛋白质,胶质,添加剂,动物饲料等

含水量0.2 mg H2O/mL

 

◆SS系列(吡啶型)


类别

货号

品名

包装

用途

规格

滴定剂

608-07025

AQUAMICRON Titrant SS 1mg (Karl Fischer Reagent SS)

容量法,一般用途,滴定剂SS 1mg,含吡啶

500 mL

适合于一般用途低含水量,溶剂是氯仿和吡啶

滴定量0.7~1.2 mg H2O/mL

601-07015

AQUAMICRON Titrant SS 3mg (Karl Fischer Reagent SS)

容量法,一般用途,滴定剂SS 3mg,含吡啶

500 mL

适合于一般用途,溶剂是氯仿和吡啶

滴定量2.53.5 mg H2O/mL

603-07215

AQUAMICRON Titrant SS 10mg (Karl Fischer Reagent SS)

容量法,一般用途,滴定剂SS 10mg,含吡啶

500 mL

一般用途高含水量,溶剂是氯仿和吡啶

滴定量812 mg H2O/mL

脱水溶

600-07085

AQUAMICRON Solvent ML

容量法溶剂,一般用途,ML溶剂

500 mL

【通用】有机溶剂、无机化学品、农用化学品,医药品,化肥,清洁剂,食品等

含水量0.2 mg H2O/mL

604-07125

AQUAMICRON Solvent MS

容量法溶剂,一般用途,含吡啶MS溶剂

500 mL

【通用】有机溶剂、无机化学品、农用化学品,医药品,化肥,清洁剂,食品等,含吡啶

含水量0.2 mg H2O/mL

603-07075

AQUAMICRON Solvent CM

容量法溶剂,用于油类CM溶剂

500 mL

【油类.油脂类用】石脑油,汽油,柴油,重油,绝缘油,油类和脂肪(硬化油,人造黄油等)等,含氯仿

含水量0.3 mg H2O/mL

604-07245

AQUAMICRON Solvent CP

容量法溶剂,用于酮类CP溶剂

500 mL

【酮类用】酮类,硅油,乙酸和其他低羟基酸,醛类(乙酰除外)等,含氯仿

含水量0.5 mg H2O/mL

606-07065

AQUAMICRON Solvent PE

容量法溶剂,用于酮类,PE溶剂

500 mL

【酮类用】酮类,硅油,乙酸和其他低羟基酸,醛类(乙酰除外)等,含吡啶

含水量0.2 mg H2O/mL

607-07095

AQUAMICRON Solvent FM

容量法溶剂,用于糖类FM溶剂

500 mL

【糖类用】糖类,蛋白质,胶质,添加剂,动物饲料等

含水量0.2 mg H2O/mL

600-07105

AQUAMICRON Solvent PP

容量法溶剂,用于醛类,PP溶剂

500 mL

【醛类】乙醛、丙醛、丁醛等,含吡啶

含水量0.2 mg H2O/mL

607-07115

AQUAMICRON Solvent ME

容量法溶剂,用于汽化器ME溶剂

500 mL

【用于汽化器】气态试样,氮气等

含水量0.2 mg H2O/mL

 


水标准试剂:可溯源到NIST SRM2890,附有分析检测成绩书。


类别

货号

品名

包装

用途

规格

容量滴定

608-07581

AQUAMICRON Water Standard 10mg

水标准品 10mg

8 m10

容量法中标定

KF试剂的滴定度

滴定量  

10±0.5 mg H2O/mL

600-07041

AQUAMICRON Standard Water Methanol Solution 2mg

水标准甲醇溶液

250 mL

逆滴定的滴定剂

标定滴定剂的滴定量

含水量  

2.0±0.01 mg H2O/mL

库伦滴定法

639-15101

AQUAMICRON® Water Standard 0.1mg

卡尔费休试剂水标准品 0.1mg

5 m10

检测库伦水分仪的精确度

滴定量

0.1±0.01 mg H2O/mL

604-07561

AQUAMICRON Water Standard 0.2mg

水标准品 0.2mg

5 m10

检测库伦水分仪的精确度

滴定量

0.2±0.01 mg H2O/mL

601-07571

AQUAMICRON Water Standard 1mg

水标准品 1mg

5 m10

检测库伦水分仪的精确度

滴定量

1±0.05 mg H2O/mL

609-07511

AQUAMICRON Check Solution P

检查溶液P

100 mL

终点调节液,可以与AX和AKX一起使用

含水量

3.8~4.2 mg H2O/mL

637-07071

AQUAMICRON Solid Water Standard

固体水标准品

10 g

用于带水分汽化法

装置水分仪

含水量

3.83±0.1%



欲了解相关资料详情请点击:

水分测试方法概述

锂电池新能源的“绊脚石”——水分

三菱化学卡尔费休试剂使用小知识


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