wako环境分析用标准品

wako环境分析用标准品

环境分析用标准品目录

货号 名称 规格
264-01541 Zinc Pyrithione Standard 500mg
206-14371 2,4,6-Triphenyl-1-hexene Standard 2,4,6-三苯基-1-己烯标准品 10mg
201-15541 2,2,3-Trihydroxydiphenyl Ether Standard 2,2,3-三羟基联苯酯标准品 100mg
208-15551 2,2,3-Trihydroxybiphenyl Standard 2,2,3-三羟基联苯标准品 100mg
203-14381 1,3,5-Triphenylcyclohexane Standard 1,3,5-三苯基环基苯标准品 10mg
208-14451 p-(1,1,3,3-Tetramethylbutyl)phenol Standard 4-(1,1,3,3-四甲基丁基)苯酚标准 500mg
169-19451 Pyrene Standard 100mg
164-19381 p-n-Pentylphenol Standard 500mg
146-06791 p-n-Nonylphenol Standard 500mg
146-06811 p-Nitrotoluene Standard 500mg
142-07511 p-Nitrophenol Standard 500mg
168-19301 1e-Phenyl-4e-(1-phenylethyl)-1,2,3,4-Tetrahydronaphthanene Standard标准品 10mg
165-19291 1e-Phenyl-4a-(1-phenylethyl)-1,2,3,4-Tetrahydronaphthanene Standard标准品 10mg
161-19271 1a-Phenyl-4e-(1-phenylethyl)-1,2,3,4-Tetrahydronaphthanene Standard标准品 10mg
168-19281 1a-Phenyl-4a-(1-phenylethyl)-1,2,3,4-Tetrahydronaphthanene Standard标准品 10mg
162-19441 Phenanthrene Standard 100mg
164-21851 Pentadecafluorooctanoic Acid Standard 全氟辛酸标准品 500mg
152-02051 Octachlorostyrene Standard 25mg
142-07491 o-Nitrophenol Standard 500mg
145-07501 m-Nitrophenol Standard 500mg
145-06881 Naphthalene Standard 100mg
089-07511 p-n-Hexylphenol Standard 500mg
082-07501 p-n-Heptylphenol Standard 500mg
066-03881 Fluorene Standard 100mg
063-03891 Fluoranthene Standard 100mg
048-26561 1,3-Diphenylpropane Standard 500mg
047-26531 trans-1,2-Diphenylcyclobutane Standard 反-1,2-二苯基环丁烷标准品 10mg
044-26541 2,4-Diphenyl-1-butene Standard 2,4-二苯基-1-丁烯标准品 10mg
049-26611 2,4-Dichlorophenol Standard 500mg
041-26791 Dibenzo[a,h]anthracene Standard 二苯并(a,h)蒽标准品 100mg
039-17881 Copper Pyrithione Standard 500mg
040-26521 cis-1,2-Diphenylcyclobutane Standard 顺-1,2-二苯基环丁烷标准品 10mg
032-17511 Chrysene Standard 100mg
028-13531 p-t-Butylphenol Standard 500mg
029-14161 p-n-Butylphenol Standard 500mg
029-13561 n-Butylbenzene Standard 500mg
025-14141 9-Bromoanthracene Standard 200mg
025-13541 Bisphenol A Standard 500mg
026-13571 Benzophenone Standard 500mg
025-13661 Benzo[k]fluoranthene Standard 100mg
022-13671 Benzo[ghi]perylene Standard 20mg
028-13651 Benzo[b]fluoranthene Standard 100mg
020-13591 Benzo[a]pyrene Standard 100mg
021-13641 Benz[a]anthracene Standard 100mg
016-18651 7 Alkylphenol Mixture Standard Solution (each 100 ug/ml Dichloromethane Solution) 7烷基酚混合物标准溶液 1mLx5A
017-17581 Acenaphthylene Standard 100mg
010-17571 Acenaphthene Standard 100mg

L-苹果酸脱氢酶[大肠杆菌] L-Malate dehydrogenase (E. coli) 货号:E-LMDHEC Megazyme中文站

L-苹果酸脱氢酶[大肠杆菌]

英文名:L-Malate dehydrogenase (E. coli)

货号:E-LMDHEC

规格:50000 Units

High purity recombinant L-Malate dehydrogenase (E. coli) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 1.1.1.37

From E. coli. This recombinant enzyme has been expressed in E. coli and purified by affinity chromatography. Electrophoretically homogeneous (MW 34,500). 
In 3.2 M ammonium sulphate. 

Specific activity: 1130 U/mg (25oC, pH 7.5. on oxaloacetic acid.

Stable at 4oC for > 4 years.

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D/L-乳酸[D-/L-乳酸盐]检测试剂盒 D/L-Lactic Acid (D-/L-Lacate) Assay Kit 货号:K-DLATE Megazyme中文站

D/L-乳酸[D-/L-乳酸盐]检测试剂盒

英文名:D/L-Lactic Acid (D-/L-Lacate) Assay Kit

货号:K-DLATE

规格:100 assays (50 of each) per kit

The D-/L-Lactic Acid (D-/L-Lactate) (Rapid) test kit is used for the rapid and specific concurrent measurement and analysis of L-lactic acid (L-lactate) and D-lactic acid (D-lactate) in beverages, meat, dairy and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

UV-method for the determination of D-/L-Lactic Acid in
foodstuffs, beverages and other materials

Principle:
(D-lactate dehydrogenase)
(1) D-Lactic acid + NAD+ ↔ pyruvate + NADH + H+

(L-lactate dehydrogenase)
(2) L-Lactic acid + NAD+ ↔ pyruvate + NADH + H+

(glutamate-pyruvate transaminase)
(3) Pyruvate + D-glutamate → D-alanine + 2-oxoglutarate

Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 10 min (L-lactic acid) and ~ 5 min (D-lactic acid)
Detection limit: 0.21 mg/L
Application examples:
Wine, soft drinks, milk, dairy products, foods containing milk (e.g. dietetic
foods, bakery products, baby food, chocolate, sweets and ice-cream),
vinegar, fruit and vegetables, processed fruit and vegetables, meat
products, food additives, paper (and cardboard), cosmetics, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by DIN, GOST,
IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAK

Advantages

  • Rapid total analysis time (concurrent / flexible D and L-lactic acid reaction format)
  • D-lactate dehydrogenase reaction very rapid with most samples (~ 5 min)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability

 

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and   therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. Is the D-/L-Lactic Acid (D-/L-Lactate) (Rapid) Assay Kit (K-DLATE) suitable for measurement using cell culture media samples?

Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit.  It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed by dilution (if required) in distilled water. 

Q5. Can perchloric acid be used to deproteinise / clarify samples prior to analysis using the D-/L-Lactic Acid Assay Kit (K-DLATE)? If so, how should such an extraction be performed?

Yes.  Perchloric acid extraction can be used in conjunction with this kit, and should be performed as follows:
WARNING: If you have not worked with perchloric acid before, you must consult your safety officer for advice.  Also, depending on the nature of the samples, it may be possible to reduce the concentration of perchloric acid, to for example 0.3 M (i.e. in the case of plasma).  It is thus very important to determine if this is possible for each type of sample used, in order to reduce the risk from working with concentrated perchloric acid.

Liquid samples:

  1. Carefully add an equal volume of ice cold 3 M perchloric acid and homogenise / fully disperse the sample (as appropriate).
  2. After 15 min incubation on ice (or in a refrigerator), centrifuge at 3000 x g for 15 min at 4°C.
  3. Neutralise by the slow addition of 2 M KOH.
  4. Incubate on ice (or in a refrigerator) until the potassium perchlorate has settled out by gravity (approximately 10 min), and then simply remove some of the clear supernatant and use directly in the assay.


Solid samples:

  1. Accurately weigh approx. 5 g of homogenised sample into a beaker containing 20 mL of 1 M perchloric acid and very carefully homogenise with an Ultraturrax® (or equivalent) for 5 min.
  2. Carefully add approx. 40 mL of distilled water and neutralise using 2 M KOH (using pH test strips for example).  Quantitatively transfer the contents to a 100 mL volumetric flask and fill to the mark with distilled water.  If a fat layer develops, make sure this is above the mark, and the aqueous layer is at the mark.
  3. Incubate in a refrigerator for approx. 20 min to allow separation of fat and precipitation of potassium perchlorate.
  4. Filter through Whatman No. 1 filter paper, discarding the first few mL of filtrate, and use directly in the assay.

Q6. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q7. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q8. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q9. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q10. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q11. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q12. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q13. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

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三磷酸腺苷Adenosine 5’-triphosphate Adenosine 5′-triphosphate (ATP) 20g 货号:C-ATP-20G Megazyme中文站

三磷酸腺苷Adenosine 5’-triphosphate

英文名:Adenosine 5′-triphosphate (ATP) 20g

货号:C-ATP-20G

规格:20g

三磷酸腺苷

High purity Adenosine 5’-triphosphate for use as a cofactor for research, biochemical enzyme assays and in vitro diagnostic analysis.

Adenosine 5’-triphosphate, disodium salt (ATP, 5’-ATP, ATP-Na2). MW: 507.18 for free acid. Purity: 97.9 %; ATP content, 82.9 %; sodium content, 7.5 %; moisture content, ~ 8 %.

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Hampton AlumaSeal™ II Sealing Film and Applicator

Hampton AlumaSeal™ II Sealing Film and Applicator

Applications

Sealing film used to reseal HT format screen kits in polypropylene blocks and plates

Features

Excellent seal
Film conforms to raised chimney wells
Easily pierceable with single or multichannel pipettors and robotic probes
Heat & cold resistant, recommended for temperatures from -80 °C to +120 °C
Certified DNase-, RNase-, and nucleic-acid-free
Less evaporation than clear films
Excellent barrier properties, virtually no reagent evaporation or drying
 Description
A 38 µm soft non-permeable aluminum foil sealing film with strong medical-grade adhesive, AlumaSeal II sealing films eliminate the need for heat-sealing devices or mats during the resealing of reagents in polypropylene deep well blocks. Each sealing film measures 82.6 x 142.9 mm and offers sufficient sealing area for 96 deep well blocks. Length between the perforations with end tabs removed is 125.4 mm. Compared to other aluminum foils, AlumaSeal II has less tendency to roll back on itself when removing the backing paper and it conforms well to the plate during application.AlumaSeal II is a soft, pierceable adhesive film designed for the convenient and rapid sealing of polypropylene deep well blocks. A multiple split backing with two end tabs allows for easy, accurate positioning and secure sealing. The use of an adhesive sealing film minimizes evaporation and helps to prevent well-to-well cross contamination in reagent blocks. AlumaSeal II films are easily pierced by pipettte tips or robotic probes or piercing tools for direct reagent recovery without significant gumming by adhesive.

AlumaSeal™ II Sealing Film and Applicator

AlumaSeal™ II Sealing Film and Applicator

CAT NO NAME DESCRIPTION
HR8-069 AlumaSeal II Sealing Film 100 pack
HR4-413 Film Sealing Paddle 5 pack

总游离亚硫酸盐检测试剂盒 Total and Free Sulphite Assay Kit 货号:K-SULPH Megazyme中文站

总游离亚硫酸盐检测试剂盒

英文名:Total and Free Sulphite Assay Kit

货号:K-SULPH

规格:40 assays (manual) / 400 assays (microplate)

A rapid, simple, reliable and accurate method for the measurement and analysis of total sulfite (sulphite) and free sulfite in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for manual, auto-analyser and microplate formats.

Suitable for manual, auto-analyser and microplate formats.

Colourimetric methods for the determination of Total and Free
Sulfite in wine, fruit juice, foodstuffs and other materials

Principle:
The Total Sulfite assay is based on the reaction principle
between thiol groups and Ellman’s reagent


The Free Sulfite assay is based on the reaction principle of
SO2, fuchsin and aldehyde binding

Kit size: 40 assays (manual) / 400 (microplate)
/ 400 (auto-analyser)
Method: Total sulfite: Spectrophotometric at 405 nm
Free sulfite: Spectrophotometric at 575 nm
Total assay time: Total sulfite: ~ 6 min
Free sulfite: ~ 9 min
Detection limit: Total sulfite: ~ 5 mg/L
Free sulfite: ~ 2 mg/L
Application examples:
Wine, fruit juice, seafood, food stuffs and other materials
Method recognition:
Validated for red and white wines at the Bundesamt für Weinbau, Austria.
Used widely in the wine industry

Advantages

  • ”Ready to use" liquid stable Formulation
  • Very competitive price (cost per test)
  • All reagents stable for > 18 months
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

 Q1. What concentration of acetaldehyde in the sample causes interference in the total sulphite (TSO2) assay?

Interference by acetaldehyde is observed when the acetaldehyde concentration is higher than approximately 250 mg/L in the 0.05 mL sample using the TSO2 Manual Assay Procedure (see Table 1). 
At acetaldehyde concentrations higher than 250 mg/L the total sulphite reaction is slower than stated in the TSO2 Manual Assay Procedure but generates the expected absorbance change when the reaction is allowed to complete.  At a concentration of 1250 mg/L acetaldehyde in the sample, the TSO2 reaction takes ~ 15 minutes to complete (at 25°C).

[Acetadehyde]

(mg/L)

[SO2]

mg/L

 

A1 

 

A2 

 

ΔAtotal SO2 

 

% error

0 

300

0.042

1.477

1.435

0.0

31 

300

0.041

1.458

1.417

1.3

63 

300

0.042

1.502

1.460

-1.7

125

300

0.042

1.498

1.456

-1.4

250

300

0.041

1.466

1.425

 0.7

625

300

0.042

1.347

1.305

 9.1

1250

300

0.045

0.279

0.234

83.7

 

 

 

 












TABLE 1. 
Acetaldehyde Interference in the TSO2 Manual Assay.  Reactions were performed using the TSO2 Manual Assay Procedure in 1 cm path-length cuvettes at 25°C.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. How stable are the sulphite standards and samples?

When the samples are prepared and are stored at the appropriate pH (~ pH 8 for total sulphite and ~ pH 4 for free sulphite) they will be expected to lose ~ 2% sulphite per hour.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. Can K-SULPH be used to measure sulphite in food samples?

Yes.  K-SULPH can be used to measure total sulphite and free sulphite in food samples using the standard sample preparation procedures given below.

Sample preparation for Total Sulphite (TSO2)
Homogenise approx. 5 g of sample with 60 mL of distilled water using a mortar and pestle or standard homogeniser for approximately 2 min.  Adjust to approximately pH 8.0 using 1 M NaOH or 1 M HCl.  Quantitatively transfer the mixture to a 100 mL volumetric flask, fill up to mark with distilled water, mix and filter through Whatman No. 1 filter paper or centrifuge at 13000 x g.  If required, dilute the sample using 20 mm sodium phosphate buffer (pH 8.0).

Sample preparation for Free Sulphite (FSO2)
Homogenise approx. 5 g of sample with 60 mL of distilled water using a mortar and pestle or standard homogeniser for approx. 2 min.  Adjust to approximately pH 4.0 using 1 M NaOH or 1 M HCl.  Quantitatively transfer the mixture to a 100 mL volumetric flask, fill up to mark with distilled water, mix and filter through Whatman No. 1 filter paper or centrifuge at 13000 x g.  If required, dilute the sample using 1% (w/v) citric acid.

Notes:
1.It is highly recommended that samples are tested immediately after sample preparation.
2.The amount of sulphite obtained in the final sample must be within the detectable range of the test.  Since the amount of sulphite present will vary between samples the amount of original sample used in the preparation method may have to be experimentally determined.

Q6. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the 
K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch 
this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q7. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q8. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q9. I have some doubts about the appearance/quality of a kit component what should be done?

视频

lumiprobe官网 cy荧光染料

Lumiprobe授权代理商–上海金畔生物

lumiprobe官网 cy 荧光染料

seline; color: rgb(55, 55, 55); text-indent: 0px;”>固话总机:021-50837765
订货热线:15221999938

seline; color: rgb(55, 55, 55); text-indent: 0px;”>qq号:2743691513 1042640511

seline; color: rgb(55, 55, 55); text-indent: 0px;”>微信号:jinpanbio
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn

seline; color: rgb(55, 55, 55); text-indent: 0px;”>Email:[email protected]

seline; color: rgb(55, 55, 55); text-indent: 0px;”>更多 www.jinpanbio.com.cn
 

lumiprobe官网中国代理商

lumiprobe官网中文 lumiprobe中国官网

热烈祝贺金畔生物正式成为Lumiprobe中国正规签约授权代理商。

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Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学用染料和其它试剂。 产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。

上金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,上海金畔生物Lumiprobe官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线,所有规格产品保证货期5-7工作日到货,满足您的各种科研实验需求。产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规(市场上部分是分装,质量不能保证),质量保证,价格极具竞争力,而且我们的货期仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

Lumiprobe染料主要用途:

NHS ESTER 适合标记氨基酸

azide 适合标记寡核苷酸和DNA
maleimide 适合标记有半胱氨酸的蛋白和多肽以及其他巯基化分子
alkyne 适合标记寡核苷酸和DNA
hydrazide 适合标记醛和酮
carboxylic acid 非活化的

lumiprobe产品按照不同作用分类:

lumiprobe荧光染料介绍

Lumiprobe 水溶性cy染料

Lumiprobe-Cyanine3.5 NHS ester

Sulfo-Cyanine5.5 NHS ester 磺酸基Cy5.5NHS

Lumiprobe-Cyanine7 NHS ester

Lumiprobe磺基cy5马来酰亚胺 Sulfo-Cyanine5 maleimide(水溶性)

Lumiprobe动物活体成像用染料|活体成像

Lumiprobe 炔烃染料 Dye alkynes

Lumiprobe 酰肼染料 Dye hydrazides

Lumiprobe羧酸类染料

Lumiprobe 羧酸染料 Carboxylic acid

Lumiprobe 马来酰亚胺染料 Dye maleimides

Lumiprobe 氨基染料 Amino dyes

Lumiprobe N-羟基琥珀酰亚胺酯染料 Dye NHS esters

Lumiprobe Cy5.5马来酰亚胺 Cyanine5.5 maleimide

Lumiprobe-Cyanine5.5 NHS ester

Lumiprobe荧光素的替代染料–菁染料

Lumiprobe SYBR Green I 染料

Lumiprobe“点击化学”用染料

Lumiproe蛋白质、多肽、核酸等生物分子标记染料

Lumiprobe蛋白质巯基标记用活性染料

lumiprobe蛋白质氨基标记用活性染料

Lumiprobe代理授权书

JinPanBio (上海金畔生物科技有限公司)

Room 103, Building 32, No.669, Dongjing Rord, Pudong New Area, Shanghai, China
Tel.: +86-21-50837765
Mobile: 86-18301939375

email: [email protected]
上海金畔生物官网:http://www.jinpanbio.cn

上海金畔生物Lumiprobe官网:http://lumiprobe.jinpanbio.com/

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

抗α-突触核蛋白抗体[ mjfr1 ](ab138501)

种属反应性

与反应:人,Drosophila melanogaster
  • 应用 AB评论 说明 WB 1 / 10000。预测分子量:分子量为14 kDa。

    对于未使用1 / 1000 – 1 / 10000

    ihc-p 1 / 150。进行热介导抗原修复免疫组化染色协议开始之前。

    对于未使用1 / 15至1 / 300。

    IHC抗原检索协议(见http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol)。

    流式细胞 1 / 200。

    对于未使用1 / 20

    ab172730兔单克隆抗体,适用于该抗体的同型对照。 IP 1 / 600。

    对于未使用1 / 50

    ELISA 使用浓度为1~10µ克/毫升。 国际商会/如果 1 / 150。

    对于未使用1 / 15

  • 靶标

    • 功能可能参与了多巴胺的释放和转运的调控。诱导微管相关蛋白tau蛋白纤维化。减少各种凋亡刺激神经元的反应性,从而降低caspase-3的激活。
    • 组织特异性主要在脑中表达也在所有检查的组织,除肝低浓度表达。集中在突触前神经末梢。
    • 疾病相关导致异常聚合成纤维SNCA基因的改变与多种神经退行性疾病相关(synucleinopathies)。SNCA纤维聚集体代表阿尔茨海默病淀粉样斑块的主要非β淀粉样蛋白的成分,与路易体包裹体的主要成分。他们也在Lewy身上发现(LB)一样的突触夹杂物,胶质细胞包涵体和轴突球状与脑内铁沉积型1神经退行性疾病。帕金森病1帕金森病4Lewy体痴呆
    • 序列相似性属于α-突触核蛋白家族。
    • 结构域“非蛋白成分阿尔茨海默病淀粉样斑块的域(NAC)参与纤维的形成。中间的疏水区域形成长丝的核心。C端可调节聚合和确定的细丝直径。
    • 翻译后修饰磷酸化丝氨酸残基,主要。磷酸化的CK1似乎残留有别于其他残基磷酸化的激酶发生。磷酸化的ser-129在突触核蛋白病病变选择性和广泛的。在体外,磷酸化在ser-129促进不溶性纤维的形成。磷酸化的tyr-125 PTK2B依赖途径在渗透胁迫。神经突触核蛋白病的标志性病变包含α-突触核蛋白是由酪氨酸残基的硝化酪氨酸交联改性和可能产生的稳定的低聚物。泛素化。主要是diubiquitinated共轭形式。乙酰化在Met-1似乎正确折叠和本土的寡聚体结构是重要的。
    • 细胞定位Cytoplasm,胞浆。膜。核。细胞连接,突触。分泌。在多巴胺能神经元的界膜。
    • 以上信息来自:UniProt加入目标p37840UniProt协会通用蛋白质资源(UniProt)2010
      核酸研究38(2010):d142-d148

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      原文地址:http://translate.baiducontent.com/transpage?cb=translateCallback&ie=utf8&source=url&query=http%3A%2F%2Fwww.abcam.cn%2Falpha-synuclein-antibody-mjfr1-ab138501.html&from=en&to=zh&token=&monLang=zh

    日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody

    日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody
    中文名称:抗丙二醛单克隆抗体
    英文名称:Anti Malondialdehyde(MDA) monoclonal antibody
    货号:MMD-030n
    规格:30ug
    Anti MDA monoclonal antibody  脂质过氧化生物标志物
    适用于免疫组化(仅供研究使用)
    About malondialdehyde (MDA)
    Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

    关于丙二醛(MDA)
    丙二醛(MDA)是衍生自脂质过氧化的主要醛之一。 MDA是高度反应性的醛,并与蛋白质中的赖氨酸残基反应。 与MDA和赖氨酸残基的反应导致众多数量的加合物的形成,例如二氢吡啶-赖氨酸(DHP-赖氨酸)型衍生物。 该单克隆抗体对MDA修饰的蛋白质,特别是DHP-赖氨酸型衍生物是特异性的。
    Specifications

    Clone #: 1F83
    Antigen: MDA-modified keyhole-lympet hemocyanine.
    Form: Frozen (100 碌g/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A.
    Application: Immunohistochemistry.
    Recommended antibody concentration is 0.5-1.0 碌g/mL on paraformaldehyde fixed tissue.
    Specificity: MSpecific for MDA-modified protein (especially DHP-lysine).
    Subclass: Mouse IgG2a(lambda)
    Storage: Less than -20掳C


    Specificity of anti MDA antibody.

    Immunohistochemical detection of MDA-modified protein in atherosclerotic aorta.
    Dr. N Shibata, Tokyo Women’s Medical University.
    技术参数
    克隆号#:1F83;
    抗原:MDA修饰的钥孔血蓝蛋白(KLH);
    性状:冻结(100μg/mL抗体在含有0.1%NaN3和0.5% BSA的10mM PBS中),经过蛋白A纯化;
    应用:免疫组织化学。对多聚甲醛固定的组织推荐抗体浓度为0.5-1.0μg/mL;
    特异性:特异性针对MDA修饰的蛋白(特别是DHP-赖氨酸)。
    亚基:小鼠IgG2a(lambda)
    储存:小于-20℃
    其他相关产品:
    ·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
    ·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
    ·  抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
    ·  己酰-赖氨酸加合物ELISA试剂盒 Hexanoyl-Lysine adduct(HEL) ELISA Kit
    References 参考文献

    1) Immunochemical detection of a lipofuscin-like fluorophore derivered from malondialdehyde and lysine.
    S Yamada, S Kumazawa, T Ishii, T Nakayama, K Itakura, N Shibata, M Kobayashi, K Sakai, T Osawa and K Uchida.
    J.Lipid Res. 42, p1187-1196 (2001)
    2) Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress.
    Muratori M, Tamburrino L, Marchiani S, Cambi M, Olivito B, Azzari C, Forti G, Baldi E
    Mol Med. 21,p109-122(2015). doi: 10.2119/molmed.2014.00158.

    日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody技术资料:http://www.jaica.com/e/technical_forum_nof.html#MDA
    日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody 说明书:
    http://www.jaica.com/e/pdf/mda_ab_manual.pdf

    氮、磷全全分析纯试剂|水质分析|环境分析纯试剂|试剂|关东化学株式会社

    横河电机㈱制的NP 600全氮磷·全自动测量装置专用的试剂,作为以下的销售的产品。另外,校正用的跨度液的各种标准液产品阵容。所以,氮和磷浓度各种调液成为可能。

    产品】【对象

    产品名 规格 产品编号
    NP 600试剂套(横河电机全氮磷·全自动测量装置用)NP 600 Reagents Set 氮、磷测量 28669 – 96
    ><套装内容

    A液 过氧二硫酸钾水溶液 一L
    B液 氢氧化钠溶液 250毫升
    C液 硫酸 250毫升
    D液 L – (+)-抗坏血酸水溶液 250毫升
    E液 钼酸铵混合溶液 250毫升
    F液 盐酸 250毫升
    容器 跨度液 250毫升

    * NP 600专用容器了,所以每更换容器试剂。
    *购买后尽量早点使用切,请避免长期保存。

    】【相关产品

    产品名 规格 包装 产品编号
    氮标准溶液(N : 1000毫克/ L)
    Nitrogen standard solution(N : 1000毫克/ L)
    水质试験用 100毫升 28670 – 23
    氮标准溶液(N : 10000毫克/ L)
    Nitrogen standard solution(N : 10000毫克/ L)
    水质试験用 100毫升 28671 – 23
    磷标准溶液(P : 10000毫克/ L)
    Phosphorus standard solution
    水质试験用 100毫升 33004 – 23
    蒸馏水
    Distilled water
    氮、磷测量 500毫升 11462 – 08
    20 L 11462 – 84

    试剂的咨询

    信息查询

    电话: 03 – 6214 – 1090

    传真: 03 – 3241 – 1047

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    PE附水龙头广口瓶


    产品编号 产品名称 产品规格 产品等级 产品价格
    2095 PE附水龙头广口瓶 5L
    2096 PE附水龙头广口瓶 10L

    PE附水龙头广口瓶

    PE附水龙头广口瓶

    容量

    5L

    10L

    螺丝尺寸

    20A

    20A

    规格

    常规

    常规

    一盒

    15个

    8个

                          *带内盖,水龙头是PP制

    AV旋塞


    产品编号 产品名称 产品规格 产品等级 产品价格
    3045 AV旋塞1型 见本页
    3046 AV旋塞2型 见本页

    AV旋塞

     

     

    AV旋塞

     

     


    原理

    规格

    简称

    连接样式

    适用尺寸

    1型

    软管/软管

    8-16mm

    2型

    凸头螺丝/软管

    螺丝口1/4  8-16mm

    ※Max.10Kgf/cm(0.98 MPa) 

     


    ◆优点・特色

     

    ●具有耐蚀性

    ●阀门由树脂制成

    ●轻便,可调节量度

     


    ◆案例・应用