T细胞尼龙毛柱分离法

上海金畔生物科技有限公司提供T细胞尼龙毛柱

简单介绍

在研究体内及体外的免疫系统时,分离淋巴细胞群是一个关键步骤。因为现在市场上并没有针对B细胞的特异性抗血清,所以分离淋巴细胞中的T细胞并不是十分方便。T细胞尼龙毛分离法利用

了尼龙毛对B细胞的亲和力,从而使T细胞在没有严重损伤的情况下达到的足够的纯度。

 

T细胞尼龙毛柱分离法的详细介绍
T细胞尼龙毛柱分离法

在研究体内及体外的免疫系统时,分离淋巴细胞群是一个关键步骤。因为现在市场上并没有针对B细胞的特异性抗血清,所以分离淋巴细胞中的T细胞并不是十分方便。T细胞尼龙毛分离法利用

了尼龙毛对B细胞的亲和力,从而使T细胞在没有严重损伤的情况下达到的足够的纯度。因为这个方法不像流式和磁珠费用昂贵,而且操作简便,所需要的条件也不高,是一种非常实用的方法

(在此我向各位解释一下,现在分离T细胞的方法主要是三种,流式,磁珠法,尼龙毛法。与前两种方法相比,尼龙毛法的优势在于无需特定的设备仪器,一般的实验室均有条件操作;价格相

对低廉;可以进行大量样本操作。)

首先要指出的是实验中所使用的是分离T细胞专用的尼龙毛(Nylon Fiber),不是化工上的尼龙纤维,曾经有人问过我这种问题,如果你买错了,可是做不出来的!其次,现在市面上的尼龙毛

也是良隽不齐,也有同仁反应买到的尼龙毛加入缓冲液以后成了一团糨糊,根本没法使用,这个问题就要靠各位的慧眼了。

现在市面上有尼龙毛填料,也已经有了商品化的尼龙毛柱子,这两者的区别主要是以下两点:

1.       商品化的柱子的填料量是已经定好的,有一个确定的上样量范围。而自己买填料装柱可以控制填料量,也就是可以控制上样量,这对于样本量非常少,或是样本量非常大的实验需求

非常合适。

2.       商品化的柱子已经经过了无菌处理(一般是辐射灭菌),而自己装柱当然就要自己灭菌了。

3.       商品化的柱子在实验的重复性上有着绝对的优势。自己装的柱子在填料的处理和装柱的松紧等方面不容易控制,差异性的控制就看你的实验技巧了。

操作方法:

1.秤取1.5g尼龙毛装入20-30ml玻璃或一次性注射器内(要可以灭菌的),填料的体积控制在15毫升左右(注射器上有标记,所以还是比较好控制的),因为尼龙毛的松紧关系到T细胞的回收率,

所以要特别注意。注射器下端配接5厘米左右长的带有弹簧夹的橡皮管。

2. 加入大量的PBS平衡柱子后,用铝箔包裹,并置于适当的容器内,高压灭菌15分钟。
(商品化尼龙毛柱以上步骤可以省略,经过无菌PBS平衡后直接进行下列步骤。)

3. 灭菌的尼龙毛柱垂直固定后放于超净台内,顶部以铝箔覆盖,避免污染。

4. 橡皮管,弹簧夹用无水酒精消毒后接注射器下端,打开弹簧夹调节流速在大约3-4ml/min。

5.首先,加入3-4倍柱体的无血培养基平衡;随后,之后加入相同体积的含有血清的培养基平衡(上述培养基都要预热),最后等培养基页面接近柱填料页面时,关闭弹簧夹。

6. 样本细胞悬浮液浓度控制在5×108cells/ml的,上样量一般是1/3-1/5柱体积(商品化的柱子一般都有推荐上样量)。样品加入之后,再加入少量培养液,然后关闭弹簧夹。

7. 柱上覆盖铝箔,移置于消毒过的容器中,37℃孵育1小时。此过程中柱子必须要保持垂直状态。

8. 从培养箱中拿出柱子,置于超净台内,除去铝箔。接上按照4.中的方法消毒的弹簧夹和橡皮管,加入适当体积经37℃预热的培养基,打开弹簧夹控制流速在3-4 mL/min,流出约5ml之后,

流出的培养液基变得不透明,此时其中含有T细胞,收集这一部分培养基。
9. 基本上何时结束收集取决于流出液体中细胞的浓度,实际上,收集的量达到一个柱体积时就可以停止收集,因为即使收集更多的量,回收率几乎不会增加。

10 。参考数据:

样本:小鼠脾脏(T细胞含量在30-40%,B细胞含量在55-60%)

细胞回收率:13-25%

B细胞污染率:小于15%

 

样本:大鼠脾脏(T细胞含量在30-35%,B细胞含量在55-65%)

细胞回收率:大于25%

B细胞污染率:小于15%

 

样本:人或兔外周血

细胞回收率 : 20-30% (人);25-35% (兔)

B细胞污染率:小于5% (人、兔)

 

(注)收集粘附于尼龙毛上的细胞时,将T细胞收集完毕后的尼龙毛在无菌操作的状态下取出,转移到适当的容器中,用镊子小心的将尼龙毛松开,粘附的细胞可以洗到培养基中。或者将注射

器芯插入注射器内,通过压力使的粘附的细胞随着液体流出。

 

产品信息

品牌     品名                   规格

Wako  T细胞尼龙毛柱          0.5g*10  (推荐用于小鼠脾脏)

Wako  T细胞尼龙毛柱L型      1g*10   (推荐用于大鼠脾脏、人或兔子的外周血)

Wako  T细胞尼龙毛            2g*5   (可按照自己的需要装柱,控制上样量)

Wako  小鼠淋巴细胞分离试剂  30mL*5  用于从小鼠脾脏,淋巴结,淋巴结悬浮液中分离淋巴细胞

Wako  人淋巴细胞分离试剂  100ml*6  用于从人血中提取淋巴细胞

Wako  铁粉(羰基铁)Iron Powder, from Iron Carbonyl  25g   直径25um,用于清除巨噬细胞

Wako  κ-卡拉胶κ-Carrageenan  25g

更多产品,更多优惠,请联系我们!
上海金畔生物科技有限公司
固话总机:021-50837765
订货热线:15221999938
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn
Email:[email protected]

 

细胞增殖因子


产品编号 产品名称 产品规格 产品等级 产品价格
白蛋白
011-21271 Albumin, from Bovine Serum (BSA), pH5.2(Fraction V)牛血清白蛋白,pH5.2 1g 和光一级
017-21273 Albumin, from Bovine Serum (BSA), pH5.2(Fraction V)牛血清白蛋白,pH5.2 10g 和光一级
019-21272 Albumin, from Bovine Serum (BSA), pH5.2(Fraction V)牛血清白蛋白,pH5.2 25g 和光一级
015-21274 Albumin, from Bovine Serum (BSA), pH5.2(Fraction V)牛血清白蛋白,pH5.2 100g 和光一级
013-21275 Albumin, from Bovine Serum (BSA), pH5.2(Fraction V)牛血清白蛋白,pH5.2 500g 和光一级
011-21276 Albumin, from Bovine Serum (BSA), pH5.2(Fraction V)牛血清白蛋白,pH5.2 1kg 和光一级
013-23291 Albumin, from Bovine Serum (BSA), Cohn Fraction V, pH7.0牛血清蛋白, pH7.0 10g 生化学用
019-23293 Albumin, from Bovine Serum (BSA), Cohn Fraction V, pH7.0牛血清蛋白, pH7.0 50g 生化学用
017-23294 Albumin, from Bovine Serum (BSA), Cohn Fraction V, pH7.0牛血清蛋白, pH7.0 100g 生化学用
015-23295 Albumin, from Bovine Serum (BSA), Cohn Fraction V, pH7.0牛血清蛋白, pH7.0 500g 生化学用
017-15146 Albumin, from Bovine Serum, Fatty Acid Free牛血清白蛋白,不含脂肪酸 5g 生化学用
017-15141 Albumin, from Bovine Serum, Fatty Acid Free牛血清白蛋白,不含脂肪酸 10g 生化学用
013-15143 Albumin, from Bovine Serum, Fatty Acid Free牛血清白蛋白,不含脂肪酸 50g 生化学用
011-15144 Albumin, from Bovine Serum, Fatty Acid Free牛血清白蛋白,不含脂肪酸 100g 生化学用
012-23381 Albumin, from Bovine Serum (BSA), pH7.0, New Zealand Origin牛血清蛋白, pH7.0, 来源于新西兰 5g 细胞培养用
010-23382 Albumin, from Bovine Serum (BSA), pH7.0, New Zealand Origin牛血清蛋白, pH7.0, 来源于新西兰 100g 细胞培养用
017-22231 30w/v% Albumin Solution, from Bovine Serum(BSA), Fatty Acid Free 50ml 细胞培养用
015-23871 30w/v% Albumin D-PBS(-) Solution, from Bovine Serum (BSA), Fatty Acid Free 50ml 细胞培养用
012-23881 7.5w/v% Albumin D-PBS(-) Solution, from Bovine Serum (BSA) 100ml 细胞培养用
013-10501 Albumin, from Human Serum人血清白蛋白 1g 生化学用
019-10503 Albumin, from Human Serum人血清白蛋白 5g 生化学用
017-10504 Albumin, from Human Serum人血清白蛋白 10g 生化学用
018-21541 Albumin, Human, recombinant expressed in plants人血清白蛋白,植物中表达的重组蛋白 1g 细胞培养用
014-21543 Albumin, Human, recombinant expressed in plants人血清白蛋白,植物中表达的重组蛋白 5g 细胞培养用
016-21542 Albumin, Human, recombinant expressed in plants人血清白蛋白,植物中表达的重组蛋白 25g 细胞培养用
胰岛素
093-06471 Insulin, Human, recombinant重组人胰岛素 50mg 细胞培养用
099-06473 Insulin, Human, recombinant重组人胰岛素 100mg 细胞培养用
097-06474 Insulin, Human, recombinant重组人胰岛素 1g 细胞培养用
093-06476 Insulin, Human, recombinant重组人胰岛素 10g 细胞培养用
090-06481 Insulin, Human, recombinant, Animal-derived-free Animal-free重组人胰岛素,无动物源成分 50mg 细胞培养用
096-06483 Insulin, Human, recombinant, Animal-derived-free Animal-free重组人胰岛素,无动物源成分 250mg 细胞培养用
094-06484 Insulin, Human, recombinant, Animal-derived-free Animal-free重组人胰岛素,无动物源成分 1g 细胞培养用
093-06351 Insulin Solution, Human, recombinant重组人胰岛素溶液 5ml 细胞培养用
转铁蛋白(Transferrin)
205-18121 Transferrin(Apo), from Human Blood转铁蛋白 100mg 细胞培养用
201-18123 Transferrin(Apo), from Human Blood转铁蛋白 1g 细胞培养用
208-18971 Transferrin(Holo), from Human Blood转铁蛋白 100mg 细胞培养用
204-18973 Transferrin(Holo), from Human Blood转铁蛋白 1mg 细胞培养用
201-18081 Transferrin, Human, recombinant expressed in plants  100mg 细胞培养用
207-18083 Transferrin, Human, recombinant expressed in plants  500mg 细胞培养用
205-18084 Transferrin, Human, recombinant expressed in plants  1g 细胞培养用
208-18091 Transferrin (Holo), from Bovine Blood, New Zealand Origin 100mg 细胞培养用

细胞培养用培养基添加物细胞增殖因子

细胞増殖因子


 

  细胞增殖的相关物质。参与各种各样的细胞生物学、生理学过程的调节,通过与靶细胞表面受容体蛋白质的特异性结合,参与细胞间的信号传递。产品取自牛、人,植物重组体、大洋洲产地原料。


白蛋白


  白蛋白是可用于酵素和成长因子等的生物学性敏感型高分子稀释·稳定剂,或可添加到脂质培养基中难溶于水的成分。


◆胰岛素


  胰岛素是对生物体内的血糖调节、促进蛋白质合成有着重要作用的激素。可作为细胞培养中的增殖因子,促进蛋白质的合成。



转铁蛋白(Transferrin)


  转铁蛋白主要负责运转培养基中所含的铁离子。

 

 相关产品


细胞培养用产品 
 
  我司为您充分准备了液体培养基如平衡盐溶液、细胞剥离·分散用溶液、抗生物质溶液、细胞外基质等产品。

神经干细胞用添加剂


  作为血清替代品用于神经干细胞的培养。我司现有两款产品,一是用Transferrin(Apo)调制而成的,一是用Transferrin(Holo)调制而成的。一般产品都含Transferrin(Holo),但是使用含有Transferrin(Apo)的N2 Supplement的话,根据细胞类型的不同神经干细胞的增殖能力可不同层次的增加。


 

 

产品编号.

品名

规格

容量

141-09041

N2 Supplement with Transferrin(Apo)(x 100)

N2神经细胞生长添加剂含转铁蛋白(Apo)(x   100)

细胞培养用

5ml

141-08941

N2 Supplement with Transferrin(Holo)(x 100)

N2神经细胞生长添加剂含转铁蛋白(Holo)(x   100)

细胞培养用

5ml

 

 

标记用辣根过氧化物酶

 

上海金畔生物科技有限公司提供辣根过氧化物酶 标记用

货号:ECE0066B
包装:100mg
英文名:Peroxidase horseradish; HRP别名:HRP级别:250u/mg

辣根过氧化物酶(HRP)是一种糖蛋白,每个分子含有一个氯化血红素(protonhemin)区作辅基。酶的浓度和纯度常以辅基的含量表示。

辣根过氧化物酶,RZ>3.0, 250u/mg,ECE0066B
英文名称:Peroxidase horseradish; HRP
CAS号:9003-99-0
EINECS号:232-668-6
Enzyme Commission Number:1.11.1.7
MDL number:MFCD00071339

级别:RZ>3.0,250u/mg
Donor:hydrogen-peroxide oxidoreductase Horseradish peroxidase

辣根过氧化物酶,英文名HRP; Peroxidase horseradish。辣根过氧化物酶是一种糖蛋白,每个分子含有一个氯化血红素(protonhemin)区作辅基。酶的浓度和纯度常以辅基的含量表示。

SKU 级别 包装 货期 价格(rmb) 其它情况
ECE0066B RZ>3.0,250u/mg 100mg 1周 询价 冰袋包装运输

更大包装请询价:15221999938
点击查看其他品牌的辣根过氧化物酶产品

保质期: 2 Years
查询产品CoA,请联系15221999938
用途
过氧化物酶,通常来源于辣根(因此称辣根过氧化物酶),是临床检验试剂中的常用酶。该产品不但广泛用于多个生化检测项目,也广泛运用于免疫类(ELISA)试剂盒。过氧化物酶作为多个

试剂盒显色体系的关键成分,对试剂盒的质量有重要影响。辣根过氧化物酶,是酶标记法所用的一种主要酶,医药上用于测定生物体液中葡萄糖和半乳糖的含量,用作诊断酶。
辣根过氧化物酶(Horseradish Peroxidase,HRP)比活性高,稳定,分子量小,纯酶容易制备,所以最常用。HRP是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,糖含量18%。HRP由多个

同功酶组成,分子量为40,000,等电点为PH3~9,酶催化的最适PH因供氢体不同而稍有差异,但多在PH5左右。酶溶于水和58%以下饱和度硫酸铵溶液。氯化血红素辅基的最大吸收峰是403nm,

HRP酶蛋白的最大吸收峰是275nm,一般以OD403nm /OD275nm的比值RZ表示酶的纯度,计算式是:
HRP纯度(RZ)=A403nm/A275nm
纯度RZ(Reinheit Zahl)值越大说明酶内所含杂质越少。高纯度的酶RZ值应在3.0左右(最高可达3.4)。用于ELISA检测的HRP的RZ值要求在3.0以上。
1) RZ>3 活性 >250u/mg, 主要应用于免疫学,是高纯度的过氧化酶。采用特殊色谱纯化技术以除去会影响免疫学反应的同工酶B .
2) RZ>2 活性 >180u/mg ,主要应用于临床化学,我们的客户也有将这个规格的产品应用于免疫学研究的。此时,一个标准化的分析方法就变得尤为重要。
3) RZ>1 活性 >100u/mg,主要应用于血糖试纸和尿液分析试纸。
4) RZ>0.6 活性 >60u/mg ,主要应用于尿液分析试纸。
安全信息
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK德国 3
F 10-21

有关该产品的运输、使用、弃置等的进一步信息,请致电15221999938索取该产品的MSDS。
贮存与运输
2-8°C。避光,防潮。

 

更多产品,更多优惠,请联系我们!
上海金畔生物科技有限公司
固话总机:021-50837765
订货热线:15221999938
qq号:2743691513
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn
Email:[email protected]

电泳缓冲液

电泳缓冲液
概述
电泳缓冲液是指在进行分子电泳时所使用的缓冲溶液,用以稳定体系酸碱度。常见的核酸电泳缓冲液有:TAE、TBE、TPE和MOPS等。
作用
缓冲液在电泳过程中的一个作用是维持合适的pH。电泳时正极与负极都会发生电解反应,正极发生的是氧化反应(4OH–4e->2H2O+O2),负极发生的是还原反应(4H++4e->2H2),长时间的电泳将使正极变酸,负极变碱。一个好的缓冲系统应有较强的缓冲能力,使溶液两极的pH保持基本不变。
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。
特点
TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量(TBE中电泳时测出的相对分子质量会大于实际分子质量),且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%,电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果,此外,回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小,长时间电泳(如过夜)不可选用,除非有循环装置使两极的缓冲液得到交换。
TBE的特点是缓冲能力强,长时间电泳时可选用TBE,并且当用于电泳小于1kb的片段时分离效果更好。TBE用于琼脂糖凝胶时易造成高电渗作用,并且因与琼脂糖相互作用生成非共价结合的四羟基硼酸盐复合物而使DNA片段的回收率降低,所以不宜在回收电泳中使用。
TPE的缓冲能力也较强,但由于磷酸盐易在乙醇沉淀过程中析出,所以也不宜在回收DNA片段的电泳中使用。
配制方法
50×TAE Buffer 配制方法:
1。称量氨基丁三醇 242g,Na2EDTA.2H2O 37.2g 于1L烧杯中;
2。向烧杯中加入约800ml去离子水,充分搅拌均匀;
3。加入57.1ml的冰乙酸,充分溶解;
4。用NaOH调pH至8.3,加去离子水定容至1L后,室温保存。
使用时稀释50倍 即1×TAE Buffer
10×TBE Buffer配制方法:
1。称量氨基丁三醇 108g,Na2EDTA.2H2O 7.44g,硼酸55g 于1L烧杯中;
2。加入约800ml去离子水,搅拌均匀;
3。用NaOH调pH至8.3,加去离子水定容至1L后,室温保存。
使用时稀释10倍 即1×TBE Buffer

10×TBE缓冲液
保存于运输说明:
RT
详细信息:

10×TBE缓冲液

10×TBE缓冲液

货号 规格 价格
M9031 500 ml 180

应用

核酸分子琼脂糖凝胶和聚丙烯酰胺凝胶电泳。

同时作为电泳缓冲液和凝胶制备缓冲液。

用于小于1500 bp RNA和DNA电泳分离。

不宜在回收电泳中使用。

注意事项

凝胶制备和电泳使用新鲜0.5×TBE缓冲液。

0.5×TBE缓冲液配制:50 ml 50×TBE与950 ml去离子水充分混匀。

双链线性核酸分子在TBE缓冲液中的迁移速度要比TAE中慢10%。

注:电泳缓冲液一般以储存液形式配制,浓度为n10×,工作时稀释n10倍,浓度为1×,而TBE储存液存放时间过长容易出现沉淀,影响电泳结果,因此配制成10×,工作浓度为

0.5×。为保持电泳所需的离子强度和pH,应经常更换电泳缓冲液。

琥珀酸/丁二酸检测试剂盒 Succinic Acid Assay Kit 货号:K-SUCC Megazyme中文站

琥珀酸/丁二酸检测试剂盒

英文名:Succinic Acid Assay Kit

货号:K-SUCC

规格:20 assays (manual) / 200 assays

The Succinic Acid test kit is suitable for the specific assay of succinic acid in wine, cheese, eggs, sauce and other food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Succinic Acid in foodstuffs,
feed, wine and other materials

Principle:
(succinyl-CoA synthetase)
(1) Succinic acid + ATP + CoA → ADP + succinyl-CoA + Pi

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → NAD+ + L-lactic acid

Kit size: 20 assays (manual) / 200 (microplate)
/ 270 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.26 mg/L
Application examples:
Wine, fruit and vegetables, soy sauce, cheese, egg, egg products
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by EEC

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction (even at room temperature)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

Q1. Is the Succinic Acid Assay Kit (K-SUCC) suitable for measurement using cell culture media samples?

Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit.  It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed by dilution (if required) in distilled water. 

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

 

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q6. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q7. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the 
K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch 
this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q8. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q9. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q10. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q11. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q12. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q14. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

视频

抗肾母细胞瘤蛋白抗体[ can-r9(IHC)- 56-2 ](ab89901)

种属反应性

与反应:老鼠,人类
  • 应用 AB评论 说明 免疫组化法 1 / 250。 流式细胞 1 / 50。

    对于未使用1 / 3。

    ab172730兔单克隆抗体,适用于该抗体的同型对照。

    WB 1 / 1000。预测分子量:分子量为55 kDa。

    对于未使用1 / 100。

    ihc-p 1 / 300。进行热三/ EDTA缓冲液pH 9开始前的免疫组化染色协议介导抗原修复。

    见协议(链路:http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol)。

    对于未使用1 / 30。

    国际商会/如果 1 / 50。

    对于未使用1 / 5。

  • 应用说明不适合IP。
  • 靶标

    • 功能转录因子,起着重要的作用,在细胞的生长和细胞存活。调节多种靶基因的表达,包括促红细胞生成素。对泌尿生殖系统的发展起着至关重要的作用。识别并结合DNA序列的5’- cgcccccgc-3”。它具有抑癌以及肿瘤形成的致癌作用。功能可能是亚型特异性:缺乏KTS主题亚型可能作为转录因子。含有KTS主题型结合mRNA和mRNA的代谢或拼接起作用。亚型1对DNA有较低的亲和力,并能结合RNA。
    • 组织特异性在肾和造血细胞表达的一个子集。
    • 疾病相关缺陷是导致WT1弗雷泽综合征(FS)[ 136680 ] MIM。FS的特点是缓慢进展性肾病导致肾功能衰竭在青春期或成年早期,男性假两性畸形,无肾母细胞瘤。对于肾脏的组织学检查发现,经常观察到局灶性肾小球硬化是。有表型重叠Denys-Drash综合征。继承是常染色体显性遗传。缺陷在WT1是肾母细胞瘤的原因1(WT1)[ 194070 ] MIM。重量是影响约1 10名婴幼儿肾胚胎性恶性肿瘤。它发生在散发性和遗传性的形式。缺陷是导致WT1 Denys-Drash综合征(DDS)[ 194080 ] MIM。DDS是一种典型的肾病以弥漫性肾小球系膜硬化,生殖器异常,和/或肾母细胞瘤。有表型重叠WAGR综合症和弗雷泽综合征。继承是常染色体显性遗传,但大多数情况下是零星的。缺陷是肾病综合征的基因4型的原因(nphs4)[ 256370 ] MIM。肾脏病临床特征为蛋白尿、低蛋白血症、高脂血症和水肿。肾活检显示非特异性的病理变化,如局灶节段性肾小球硬化和弥漫性系膜增殖。一些受影响的人有遗传性激素耐药的形成和进展至终末期肾功能衰竭。大多数患者nphs4显示弥漫性肾小球硬化症肾活检,这是一种病理特征无系膜hypercellularity肾小球系膜扩张、肥厚的足细胞,足细胞空泡化,基底膜增厚,毛细血管管腔通畅的减少。缺陷是引起米查姆综合征基因(meachs)[ 608978 ] MIM。米查姆综合征是一种罕见的零星发生多发畸形综合征的特点是男性假两性畸形与异常的内在女性生殖器包括子宫、双阴道纵隔、复杂先天性心脏缺损的膈肌异常。注=一个染色体畸变涉及WT1可促结缔组织增生性小圆细胞肿瘤(DSRCT)的原因。易位t(11;22)(P13;q12)和EWSR1。
    • 序列相似性属于EGR C2H2型锌指蛋白家族。含有4个C2H2型锌指。
    • 细胞定位核。细胞质.细胞核和细胞质之间穿梭;核>核质和核散斑。

    Lumiprobe 水溶性cy染料

    Lumiprobe 水溶性Cy染料

    磺化和非磺化染料都可用于标记生物分子例如DNA和蛋白质。染料之间的差异是它们的溶解性:磺基染料是水溶性的,并且它们不使用有机共溶剂用于在水性环境中的标记。它们不易于在水中聚集。

    There are two varieties of cyanine dyes: non-sulfonated cyanines, and sulfonated cyanines. For many applications they are interchangeable, because their spectral properties are nearly identical. Both sulfonated and non-sulfonated dyes can be used for the labeling of biomolecules such as DNA and proteins. The difference between the dyes is their solubility: sulfo- dyes are water-soluble, and they do not use of organic co-solvent for the labeling in aqueous environment. They are less prone to aggregation in water.

    • Sulfo-Cyanine3 carboxylic acid
    • Sulfo-Cyanine5 carboxylic acid
    • Sulfo-Cyanine5.5 carboxylic acid
    • Sulfo-Cyanine7 carboxylic acid
    • Sulfo-Cyanine7.5 carboxylic acid
    磺酸基-Cy5羧酸

    无活性的磺酸基-Cy5羧酸,水溶性染料。此染料是高水溶性染料
    用于分子标记应该选择具有活性的磺酸基-Cy5 NHS酯

    分子结构:

    一般性质:
    外观:深蓝色粉末
    分子量:664.76
    分子式:C32H37N2NaO8S2
    溶解性:易溶于水,DMSO,DMF,在多数有机溶剂难溶
    质控:NMR 1H(95%)和13C,TLC

    光谱性质:
    最大激发波长:646nm
    在最大激发波长下的消光系数:271000Lmol-1cm-1
    最大发射波长:662nm
    荧光量子产率:0.2

    General properties

    Appearance: dark blue powder
    Molecular weight: 664.76
    CAS number: 1121756-16-8, 1144162-77-5
    Molecular formula: C32H37N2NaO8S2
    Solubility: Good in water, DMF, DMSO. Low in non-polar organic solvents.
    Quality control: NMR 1H, HPLC-MS (95%)
    Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

    sulfo-Cyanine5 carboxylic acid structure

    sulfo-Cyanine5 absorbance and emission spectra

    Cat. # Quantity Lead time
    13390 1 mg in stock
    23390 5 mg in stock
    43390 25 mg in stock
    53390 50 mg in stock
    63390 100 mg in stock
     磺酸基-Cy7羧酸

    磺酸基-Cy7羧酸是无活性易溶于水的近红外染料;
    此试剂不能用于标记分子,它有较高的水溶性,可改善近红外光谱范围的量子产率,并具有很高的消光系数。

    分子结构:

    一般性质:
    外观:深绿色粉末
    分子量:730.87
    分子式:C37h33N2NaO8S2
    溶解性:易溶于水,DMF,DMSO
    质控:HPLC(95%),NMR 1H 和13C

    光谱性质:
    最大激发波长:740nm
    在最大激发波长下的消光系数:240600Lmol-1cm-1
    最大发射波长:773nm

    General properties

    Appearance: dark green powder
    Molecular weight: 730.87
    Molecular formula: C37H43N2NaO8S2
    Solubility: Good in water, DMF, DMSO. Low in non-polar organic solvents.
    Quality control: NMR 1H, HPLC (95%)
    Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

    Sulfo-Cyanine7 carboxylic acid is non-reactive water soluble near infrared dye.

    The reagent is useful as a fluorescent marker in NIR range when attachment to other molecules is not desired. It has high hydrophilicity and aqueous solubility, improved quantum yield in NIR range, and very high molar extinction coefficient.

    This reagent contains free carboxylic acid function. Pre-activated activated ester – sulfo-Cyanine7 NHS ester is also available.

    15390 1 mg in stock
    25390 5 mg in stock
    45390 25 mg in stock
    55390 50 mg in stock
    65390 100 mg in stock
    磺基-Cy3 NHS酯:

    此染料可溶于水,氨基活性Cy3染料。在水溶液中有效标记蛋白和多肽,无需共溶剂,是低溶解性蛋白和易于降解蛋白的理想选择。
    此染料是磺化,亲水性染料。可以代替Alexa Fluor 546和DyLight 549

    分子结构:
    一般性质:
    外观:深红色晶体
    分子量:735.8
    分子式:C34H38N3NaO10S2
    溶解性:极易溶于水,易溶于有机溶剂(DMF,DMSO)
    质控:NMR 1H(95%)和13C,TLC,功能性测试

    光谱性质:
    最大激发波长:548nm
    最大激发波长下的消光系数:162000Lmol-1cm-1
    最大发射波长:563nm
    荧光量子产率:0.1

    General properties

    Appearance: dark red crystals
    Molecular weight: 735.80
    Molecular formula: C34H38N3NaO10S2
    Solubility: very high in water, good in polar organic solvents (DMF, DMSO)
    Quality control: NMR 1H, HPLC-MS (95%)
    Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

    Water soluble sulfo-Cy3 NHS ester structure

    sulfo-Cyanine3 absorbance and emission spectra

    11320 1 mg in stock
    21320 5 mg in stock
    41320 25 mg in stock
    51320 50 mg in stock
    61320 100 mg in stock
    磺基-Cy5 NHS酯:

    水溶性Cy5 NHS酯,用于标记生物分子的氨基,无需任何共溶剂,因此可以用于标记在有机溶剂中易降解的蛋白和溶解性低的蛋白
    Cy5 是最常用的荧光团之一,与多种设备兼容,比如平板读数仪,显微镜和成像仪
    此染料是高水溶性,可以代替多种用途的Alexa Fluor 647,DyLight 649

    分子结构:
    一般性质:
    外观:深蓝色粉末
    分子量:761,84
    分子式:C36H40N3NaO10S2
    溶解性:极易溶于水,易溶于有机溶剂DMF和DMSO
    质量控制:NMR 1H (95%)和13C,TLC,功能性测试

    光谱性质:
    最大激发波长:646nm
    最大激发波长下的消光系数:271000Lmol-1cm-1
    最大发射波长:662nm
    荧光量子产率:0.2

    General properties

    Appearance: dark blue powder
    Molecular weight: 777.95
    Molecular formula: C36H40N3NaO10S2
    Solubility: very good in water, good in DMF and DMSO
    Quality control: NMR 1H, HPLC-MS (95%)
    Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

    sulfo-cy5-nhs-ester

    Cy5 NHS ester structure

    sulfo-Cyanine 5 absorbance and emission spectra

    13320 1 mg in stock
    23320 5 mg in stock
    43320 25 mg in stock
    53320 50 mg in stock
    63320 100 mg in stock
    磺基-Cy7 NHS酯:

    一般性质:
    外观:深绿色粉末
    分子量:827.94
    分子式:C41H46N3NaO10S2
    溶解性:易溶于水,DMF,DMSO
    质控:NMR 1H(95%)13C,TLC,功能测试

    光谱性质:
    最大激发波长:740nm
    在最大激发波长下的消光系数:240600Lmol-1cm-1
    最大发射波长:773nm

    General properties

    Appearance: dark green powder
    Mass spec M+ increment: 868.2
    Molecular weight: 869.10
    Molecular formula: C43H49KN4O9S2
    IUPAC name: 1-[2-(6-{2-[(E)-2-[(E)-3-{(E)-2-[1-Methyl-3,3-dimethyl-5-(oxysulfonyl)-2-indolinylidene]ethylidene}-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-5-(oxysulfonyl)-3H-indol-1-yl}hexanoylamino)ethyl]-1H-pyrrole-2,5-dione, potassium salt
    Solubility: Good in water, DMF, DMSO. Low in non-polar organic solvents.
    Quality control: NMR 1H, HPLC-MS (95%)
    Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

    sulfo-Cyanine7 maleimide structure

    sulfo-Cyanine7 absorbance and emission spectra

    15380 1 mg in stock
    25380 5 mg 5 days
    45380 25 mg in stock
    55380 50 mg in stock
    Sulfo-Cyanine5 maleimide   磺基-cy5-马来酰亚胺

    水溶的亲水的sulfo-Cyanine5 maleimide(Cy5® maleimide的类似物)。作为Cy5® maleimide的完美的替代物,我们推荐该产品用于蛋白、包括抗体的标记。通过凝胶过滤、离心柱纯化、透析、电泳或色谱层析方法就可以很容易的将标记蛋白和未标记的染料进行分类。
    没有磺化基团的类似物:Cyanine5 maleimide。

    磺酸基类荧光染料:
    • Sulfo-Cyanine3 NHS ester
    • Sulfo-Cyanine5 NHS ester
    • Sulfo-Cyanine5 bis-NHS ester
    • Sulfo-Cyanine5.5 NHS ester
    • Sulfo-Cyanine7 NHS ester
    • Sulfo-Cyanine3 maleimide
    • Sulfo-Cyanine5 maleimide
    • Sulfo-Cyanine7 maleimide
    • Sulfo-Cyanine3 azide
    • Sulfo-Cyanine5 azide
    • Sulfo-Cyanine5.5 azide
    • Sulfo-Cyanine7 azide
    • Sulfo-Cyanine3 alkyne
    • Sulfo-Cyanine5 alkyne
    • Sulfo-Cyanine5.5 alkyne
    • Sulfo-Cyanine7 alkyne
    • Sulfo-Cyanine3 amine
    • Sulfo-Cyanine5 amine
    • Sulfo-Cyanine5.5 amine
    • Sulfo-Cyanine7 amine

    MPEG-NH2-5000-1g/5g 甲氧基聚乙二醇-氨基,Mw:5000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-ACRL-5000-1g/5g        甲氧基聚乙二醇-丙烯酸酯,Mw:5000

    MPEG-ACRL-1000-1g     甲氧基聚乙二醇-丙烯酸酯,Mw:1000

    MPEG-ACRL-2000-1g     甲氧基聚乙二醇-丙烯酸酯,Mw:2000

    MPEG-ACRL-10K-1g/5g 甲氧基聚乙二醇-丙烯酸酯,Mw:10K

    ACRL-PEG-ACRL-3400-1g       丙烯酸酯-聚乙二醇-丙烯酸酯,Mw:3400

    修饰性PEG聚乙二醇衍生物系列

    ACRL-PEG-ACRL-3400-5g       丙烯酸酯-聚乙二醇-丙烯酸酯,Mw:3400

    ACRL-PEG-ACRL-5000-1g/5g         丙烯酸酯-聚乙二醇-丙烯酸酯,Mw:5000

    ACRL-PEG-ACRL-10K-1g/5g   丙烯酸酯-聚乙二醇-丙烯酸酯,Mw:10K

    ACRL-PEG-ACRL-20K-1g/5g   丙烯酸酯-聚乙二醇-丙烯酸酯,Mw:20K

    ACRL-PEG-SVA-3400-500mg/1g/5g      丙烯酸酯-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:3400

    4arm-PEG-ACRL-10K-1g/5g   4arm-聚乙二醇-丙烯酸酯,Mw:10K

    ACRL-PEG-ACRL-4MW Kit      丙烯酸酯-聚乙二醇-丙烯酸酯-4MW Kit

    修饰性PEG聚乙二醇衍生物系列

    ACRL-PEG-MAL-3400-500mg/1g  丙烯酸酯-聚乙二醇-马来酰亚胺,Mw:3400

    ACRL-PEG-MAL-5000-500mg/1g  丙烯酸酯-聚乙二醇-马来酰亚胺,Mw:5000

    ACRL-PEG-SVA-5000-500mg/1g    丙烯酸酯-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:5000

    ACRL-PEG-SVA-2000-500mg/1g    丙烯酸酯-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:2000

    ACRL-PEG-SVA-8000-500mg  丙烯酸酯-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:8000

    4arm-PEG-ACRL-20K-5g/1g   4arm-聚乙二醇-丙烯酸酯,Mw:20K

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-bALD-2K-1g/5g   甲氧基聚乙二醇-丁醛,Mw:2K

    MPEG-bALD-5K-1g/5g   甲氧基聚乙二醇-丁醛,Mw:5K

    MPEG-bALD-10K-1g        甲氧基聚乙二醇-丁醛,Mw:10K

    MPEG-bALD-20K-1g/5G 甲氧基聚乙二醇-丁醛,Mw:20K

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-bALD-30K-1g/5g 甲氧基聚乙二醇-丁醛,Mw:30K

    bALD-PEG-bALD-3400-1g/5g         丁醛-聚乙二醇-丁醛,Mw:3400

    bALD-PEG-SVA-3400-1g 丁醛-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:3400

    MPEG-bALD-4MW Kit     甲氧基聚乙二醇-丁醛-4MW Kit

    MPEG-pALD-1000-1g     甲氧基聚乙二醇-丙醛,Mw:1000

    MPEG-pALD-2000-1g/-5g      甲氧基聚乙二醇-丙醛,Mw:2000

    MPEG-pALD-5000-1g/5g        甲氧基聚乙二醇-丙醛,Mw:5000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-pALD-20K-1g/5g 甲氧基聚乙二醇-丙醛,Mw:20K

    MPEG-pALD-30K-1g/5g 甲氧基聚乙二醇-丙醛,Mw:30K

    bALD-PEG-bALD-10K-1g/5g   丁醛-聚乙二醇-丁醛,Mw:10K

    bALD-PEG-bALD-20K-1g/5g   丁醛-聚乙二醇-丁醛,Mw:20K

    bALD-PEG-bALD-35K-1g/5g   丁醛-聚乙二醇-丁醛,Mw:35K

    bALD-PEG-SVA-2000-1g 丁醛-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:2000

    bALD-PEG-VA-3400-1g/5g     丁醛-聚乙二醇-戊酸 ,Mw:3400

    MPEG-NH2-2000-1g/5g         甲氧基聚乙二醇-氨基,Mw:2000

    MPEG-NH2-5000-1g/5g         甲氧基聚乙二醇-氨基,Mw:5000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-NH2-10K-1g/5g   甲氧基聚乙二醇-氨基,Mw:10K

    MPEG-NH2-20K-1g/5g   甲氧基聚乙二醇-氨基,Mw:20K

    MPEG-NH2-30K-1g/5g   甲氧基聚乙二醇-氨基,Mw:30K

    NH2-PEG-NH2-2K-1g      氨基-聚乙二醇-氨基,Mw:2K

    NH2-PEG-NH2-3400-1g/5g   氨基-聚乙二醇-氨基,Mw:3400

    NH2-PEG-NH2-5K-1g/5g        氨基-聚乙二醇-氨基,Mw:5K

    修饰性PEG 聚乙二醇衍生物系列

    NH2-PEG-NH2-10K-1g/5g      氨基-聚乙二醇-氨基,Mw:10K

    NH2-PEG-NH2-20K-1g/5g      氨基-聚乙二醇-氨基,Mw:20K

    NH2-PEG-VA-3400-500mg/1g       氨基-聚乙二醇-戊酸 ,Mw:3400

    NH2-PEG-VA-5K-500mg/1g   氨基-聚乙二醇-戊酸 ,Mw:5K

    Biotin-PEG-NH2-3400-1g-100mg/500mg     生物素-聚乙二醇-氨基,Mw:3400

    DSPE-PEG-NH2-3400-1g/5g  DSPE-聚乙二醇-氨基,Mw:3400

    DSPE-PEG-NH2-3400-500mg DSPE-聚乙二醇-氨基,Mw:3400

    HO-PEG-NH2-3400-1g/5g      HO-聚乙二醇-氨基,Mw:3400

    HO-PEG-NH2-3400-500mg    HO-聚乙二醇-氨基,Mw:3400

    修饰性PEG 聚乙二醇衍生物系列

    BOC-NH-PEG-NH2-3400-1g/5g     BOC-NH-聚乙二醇-氨基,Mw:3400

    BOC-NH-PEG-NH2-3400-500mg   BOC-NH-聚乙二醇-氨基,Mw:3400

    4arm-PEG-NH2-10K-1g/5g    4arm-聚乙二醇-氨基,Mw:10K

    MPEG-NH2-4MW Kit      甲氧基聚乙二醇-氨基,4MW Kit

    MPEG-NH2-550-1g/5g   甲氧基聚乙二醇-氨基,Mw:550

    MPEG-NH2-1000-1g/5g         甲氧基聚乙二醇-氨基,Mw:1000

    NH2-PEG-NH2-550-1g   氨基-聚乙二醇-氨基,Mw:550

    修饰性PEG 聚乙二醇衍生物系列

    NH2-PEG-NH2-1K-1g/5g        氨基-聚乙二醇-氨基,Mw:1K

    NH2-PEG-NH2-35K-1g/5g      氨基-聚乙二醇-氨基,Mw:35K

    NH2-PEG-CM-2000-500mg/1g      氨基-聚乙二醇-羧甲基,Mw:2000

    NH2-PEG-CM-3400-1g/5g/500mg        氨基-聚乙二醇-羧甲基,Mw:3400

    NH2-PEG-CM-5000-1g/500mg      氨基-聚乙二醇-羧甲基,Mw:5000

    NH2-PEG-SH-1000-1g/500mg       氨基-聚乙二醇-巯基,Mw:1000

    修饰性PEG 聚乙二醇衍生物系列

    NH2-PEG-SH-10K-1g/500mg 氨基-聚乙二醇-巯基,Mw:10K

    Biotin-PEG-NH2-550-1g/500mg    生物素-聚乙二醇-氨基,Mw:550

    Biotin-PEG-NH2-2K-1g/100mg/500mg         生物素-聚乙二醇-氨基,Mw:2K

    Biotin-PEG-NH2-5000-1g/100mg/500mg    生物素-聚乙二醇-氨基,Mw:5000

    DSPE-PEG-NH2-2000-1g/500mg   DSPE-聚乙二醇-氨基,Mw:2000

    DSPE-PEG-NH2-5000-1g/500mg   DSPE-聚乙二醇-氨基,Mw:5000

    FMOC-NH-PEG-NH2-TFA-1K-1g/5g       FMOC-NH-聚乙二醇-氨基-TFA,Mw:1K

    修饰性PEG 聚乙二醇衍生物系列

    FMOC-NH-PEG-NH2-TFA-5K-1g/5g       FMOC-NH-聚乙二醇-氨基-TFA,Mw:5K

    FMOC-NH-PEG-NH2-TFA-5K-500mg     FMOC-NH-聚乙二醇-氨基-TFA,Mw:5K

    HO-PEG-NH2-1000-1g/100mg      HO-聚乙二醇-氨基,Mw:1000

    HO-PEG-NH2-1000-500mg/1g      HO-聚乙二醇-氨基,Mw:1000

    HO-PEG-NH2-2000-500mg    HO-聚乙二醇-氨基,Mw:2000

    HO-PEG-NH2-5000-1g/500mg      HO-聚乙二醇-氨基,Mw:5000

    修饰性PEG 聚乙二醇衍生物系列

    HO-PEG-NH2-20K-1g/500mg         HO-聚乙二醇-氨基,Mw:20K

    HO-PEG-NH2-35K-1g/500mg         HO-聚乙二醇-氨基,Mw:35K

    BOC-PEG-NH2-1000-1g/5g   BOC-聚乙二醇-氨基,Mw:1000

    BOC-NH-PEG-NH2-5K-1g/5g/500mg    BOC-NH-聚乙二醇-氨基,Mw:5K

    4arm-PEG-NH2-20K-1g/5g    4arm-聚乙二醇-氨基,Mw:20K

    BOC-PEG-NH2-1000-1g/5g   BOC-聚乙二醇-氨基,Mw:1000

    BOC-NH-PEG-NH2-5K-1g/5g/500mg    BOC-NH-聚乙二醇-氨基,Mw:5K

    修饰性PEG 聚乙二醇衍生物系列

    4arm-PEG-NH2-20K-1g/5g    4arm-聚乙二醇-氨基,Mw:20K

    MPEG-Biotin-2000-1g/5g      甲氧基聚乙二醇-生物素,Mw:2000

    MPEG-Biotin-5000-1g/5g      甲氧基聚乙二醇-生物素,Mw:5000

    MPEG-Biotin-10K-1g      甲氧基聚乙二醇-生物素,Mw:10K

    MPEG-Biotin-20K-1g/5g         甲氧基聚乙二醇-生物素,Mw:20K

    Biotin-PEG-DSPE-3400-1g/500mg         生物素-聚乙二醇-DSPE,Mw:3400

    Biotin-PEG-DSPE-3400-500mg       生物素-聚乙二醇-DSPE,Mw:3400

    Biotin-PEG-MAL-3400-1g/500mg/100mg    生物素-聚乙二醇-马来酰亚胺,Mw:3400

    Biotin-PEG-NH2-3400-1g/100mg/500mg    生物素-聚乙二醇-氨基,Mw:3400

    修饰性PEG 聚乙二醇衍生物系列

    Biotin-PEG-SIL-3400-1g/500mg    生物素-聚乙二醇-硅烷,Mw:3400

    Biotin-PEG-SC-3400-1g  生物素-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:3400

    Biotin-PEG-SC-3400-100mg/500mg      生物素-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:3400

    Biotin-PEG-SC-5000-100mg/1g     生物素-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:5000

    Biotin-PEG-SVA-3400-1g/100mg/500mg      生物素-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:3400

    Biotin-PEG-SVA-5000-1g/100mg/500mg      生物素-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:5000

    Biotin-PEG-Biotin-3400-1g    生物素-聚乙二醇-生物素,Mw:3400

    修饰性PEG 聚乙二醇衍生物系列

    Biotin-PEG-DSPE-1000-1g/500mg         生物素-聚乙二醇-DSPE,Mw:1000

    Biotin-PEG-DSPE-2000-1g      生物素-聚乙二醇-DSPE,Mw:2000

    Biotin-PEG-MAL-5000-1g/100mg/500mg    生物素-聚乙二醇-马来酰亚胺,Mw:5000

    Biotin-PEG-MAL-10K-1g/100mg/500mg      生物素-聚乙二醇-马来酰亚胺,Mw:10K

    Biotin-PEG-SC-2000-1g/100mg/500mg        生物素-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:2000

    Biotin-PEG-SVA-10K-1g/100mg/500mg        生物素-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:10K

    Biotin-PEG-SVA-20K-1g/100mg/500mg        生物素-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:20K

    Biotin-PEG-SIL-5K-1g      生物素-聚乙二醇-硅烷,Mw:5K

    ASA-PEG-ASA-3400-1g   氨基琥珀酸-聚乙二醇-氨基琥珀酸,Mw:3400

    修饰性PEG 聚乙二醇衍生物系列

    CM-PEG-SH-3400-1g/5g/500mg   羧甲基-聚乙二醇-巯基,Mw:3400

    CM-PEG-SH-5000-1g/5g/500mg   羧甲基-聚乙二醇-巯基,Mw:5000

    HO-PEG-CM-3400-1g/5g/500mg  HO-聚乙二醇-羧甲基,Mw:3400

    4arm-PEG-ASA-10K-1g   4arm-聚乙二醇-氨基琥珀酸,Mw:10K

    MPEG-CM-2000-1g/5g  甲氧基聚乙二醇-羧甲基,Mw:2000

    MPEG-CM-5000-1g/5g  甲氧基聚乙二醇-羧甲基,Mw:5000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-CM-20K-1g/5g    甲氧基聚乙二醇-羧甲基,Mw:20K

    MPEG-CM-10K-1g/5g    甲氧基聚乙二醇-羧甲基,Mw:10K

    ASA-PEG-ASA-20K-1g     氨基琥珀酸-聚乙二醇-氨基琥珀酸,Mw:20K

    CM-PEG-CM-1000-1g/5g       羧甲基-聚乙二醇-羧甲基,Mw:1000

    CM-PEG-CM-2000-1g/5g       羧甲基-聚乙二醇-羧甲基,Mw:2000

    CM-PEG-CM-5000-1g/5g       羧甲基-聚乙二醇-羧甲基,Mw:5000

    CM-PEG-DSPE-2000-500mg  羧甲基-聚乙二醇-DSPE,Mw:2000

    修饰性PEG聚乙二醇衍生物系列

    CM-PEG-DSPE-5000-500mg  羧甲基-聚乙二醇-DSPE,Mw:5000

    CM-PEG-SCM-35K-1g/5g       羧甲基-聚乙二醇-琥珀酰亚胺碳酸酯M,Mw:35K

    CM-PEG-SH-2000-1g/500mg         羧甲基-聚乙二醇-巯基,Mw:2000

    FMOC-NH-PEG-CM-3400-1g/500mg    FMOC-NH-聚乙二醇-羧甲基,Mw:3400

    FMOC-NH-PEG-CM-5000-1g/5g/500mg      FMOC-NH-聚乙二醇-羧甲基,Mw:5000

    HO-PEG-CM-2000-1g/500mg        HO-聚乙二醇-羧甲基,Mw:2000

    修饰性PEG 聚乙二醇衍生物系列

    HO-PEG-CM-5000-1g/500mg        HO-聚乙二醇-羧甲基,Mw:5000

    SC-PEG-CM-3400-1g/500mg 琥珀酰亚胺碳酸酯-聚乙二醇-羧甲基,Mw:3400

    SC-PEG-CM-5000-500mg       琥珀酰亚胺碳酸酯-聚乙二醇-羧甲基,Mw:5000

    4arm-PEG-ASA-20K-1g   4arm-聚乙二醇-氨基琥珀酸,Mw:20K

    MPEG-EPOX-2000-1g     甲氧基聚乙二醇-环氧基,Mw:2000

    MPEG-EPOX-5000-1g/5g        甲氧基聚乙二醇-环氧基,Mw:5000

    EPOX-PEG-EPOX-2K-1g/5g     环氧基-聚乙二醇-环氧基,Mw:2K

    修饰性PEG聚乙二醇衍生物系列

    EPOX-PEG-EPOX-5K-1g/5g     环氧基-聚乙二醇-环氧基,Mw:5K

    4arm-PEG-EPOX-10K-1g/5g   4arm-聚乙二醇-环氧基,Mw:10K

    4arm-PEG-EPOX-20K-1g/5g   4arm-聚乙二醇-环氧基,Mw:20K

    MPEG-SAS-10K-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺氨基琥珀酸酯  ,Mw:10K

    mPEG-SAS-5K-1g/5g       甲氧基聚乙二醇-琥珀酰亚胺氨基琥珀酸酯  ,Mw:5K

    mPEG-SAS-2K-1g/5g       甲氧基聚乙二醇-琥珀酰亚胺氨基琥珀酸酯  ,Mw:2K

    MPEG-SAS-20K-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺氨基琥珀酸酯  ,Mw:20K

    mPEG-SC-2K-1g/5g         甲氧基聚乙二醇-琥珀酰亚胺碳酸酯,Mw:2K

    MPEG-SC-5000-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺碳酸酯,Mw:5000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-SC-10K-1g/5g      甲氧基聚乙二醇-琥珀酰亚胺碳酸酯,Mw:10K

    MPEG-SC-20K-1g/5g      甲氧基聚乙二醇-琥珀酰亚胺碳酸酯,Mw:20K

    MPEG-SG-5000-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺戊二酸酯 ,Mw:5000

    MPEG-SS-2000-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺琥珀酸酯 ,Mw:2000

    MPEG-SS-5000-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺琥珀酸酯 ,Mw:5000

    MPEG-SS-10K-1g/5g       甲氧基聚乙二醇-琥珀酰亚胺琥珀酸酯 ,Mw:10K

    MPEG-SS-20K-1g/5g       甲氧基聚乙二醇-琥珀酰亚胺琥珀酸酯 ,Mw:20K

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-SVA-2000-1g/5g  甲氧基聚乙二醇-琥珀酰亚胺戊酸酯,Mw:2000

    MPEG-SVA-5000-1g/5g  甲氧基聚乙二醇-琥珀酰亚胺戊酸酯,Mw:5000

    MPEG-SVA-10K-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺戊酸酯,Mw:10K

    MPEG-SVA-20K-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺戊酸酯,Mw:20K

    MPEG-SVA-30K-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺戊酸酯,Mw:30K

    SAS-PEG-SAS-3400-1g/5g      琥珀酰亚胺氨基琥珀酸酯-聚乙二醇-琥珀酰亚胺氨基琥珀酸酯  ,Mw:3400

    修饰性PEG 聚乙二醇衍生物系列

    SG-PEG-SG-3400-1g/5g 琥珀酰亚胺戊二酸酯 -聚乙二醇-琥珀酰亚胺戊二酸酯,Mw:3400

    SS-PEG-SS-3400-1g/5g   琥珀酰亚胺琥珀酸酯 -聚乙二醇-琥珀酰亚胺琥珀酸酯,Mw:3400

    SVA-PEG-SVA-3400-1g/5g      琥珀酰亚胺戊酸酯-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:3400

    BOC-NH-PEG-SC-3400-5g/500mg         BOC-NH-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:3400

    BOC-NH-PEG-SVA-3400-1g/5g/500mg         BOC-NH-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:3400

    FMOC-NH-PEG-SVA-3400-1g/5g/500mg      FMOC-NH-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:3400

    MAL-PEG-SVA-3400-1g/100mg/500mg        马来酰亚胺-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:3400

    OPSS-PEG-SVA-5000-1g/500mg    OPSS-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:5000

    4arm-PEG-SAS-10K-1g   4arm-聚乙二醇-琥珀酰亚胺氨基琥珀酸酯  ,Mw:10K

    修饰性PEG 聚乙二醇衍生物系列

    4arm-PEG-SC-10K-1g     4arm-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:10K

    4arm-PEG-SG-10K-1g/5g       4arm-聚乙二醇-琥珀酰亚胺戊二酸酯 ,Mw:10K

    4arm-PEG-SS-10K-1g/5g        4arm-聚乙二醇-琥珀酰亚胺琥珀酸酯 ,Mw:10K

    MPEG-SG-2000-1g/5g    甲氧基聚乙二醇-琥珀酰亚胺戊二酸酯 ,Mw:2000

    MPEG-SG-10K-1g/5g      甲氧基聚乙二醇-琥珀酰亚胺戊二酸酯 ,Mw:10K

    MPEG-SG-20K-1g/5g      甲氧基聚乙二醇-琥珀酰亚胺戊二酸酯 ,Mw:20K

    MPEG-SG-30K-1g/5g      甲氧基聚乙二醇-琥珀酰亚胺戊二酸酯 ,Mw:30K

    MPEG-SPA-550-1g/5g    甲氧基聚乙二醇-SPA,Mw:550

    MPEG-SPA-1000-1g/5g  甲氧基聚乙二醇-SPA,Mw:1000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-SS-30K-1g/5g       甲氧基聚乙二醇-琥珀酰亚胺琥珀酸酯 ,Mw:30K

    SAS-PEG-SAS-2000-1g/5g      琥珀酰亚胺氨基琥珀酸酯-聚乙二醇-琥珀酰亚胺氨基琥珀酸酯  ,Mw:2000

    SG-PEG-SG-5K-1g/5g      琥珀酰亚胺戊二酸酯-聚乙二醇-琥珀酰亚胺戊二酸酯 ,Mw:5K

    SG-PEG-SG-10K-1g/5g   琥珀酰亚胺戊二酸酯-聚乙二醇-琥珀酰亚胺戊二酸酯 ,Mw:10K

    SS-PEG-SS-2000-1g         琥珀酰亚胺琥珀酸酯 -聚乙二醇-琥珀酰亚胺琥珀酸酯,Mw:2000

    SVA-PEG-SVA-1000-1g/5g      琥珀酰亚胺戊酸酯-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:1000

    SVA-PEG-SVA-5K-1g/5g  琥珀酰亚胺戊酸酯-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:5K

    修饰性PEG 聚乙二醇衍生物系列

    FMOC-NH-PEG-SC-2000-1g/500mg      FMOC-NH-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:2000

    FMOC-NH-PEG-SVA-5K-1g/500mg        FMOC-NH-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:5K

    MAL-PEG-SVA-5000-100mg/500mg      马来酰亚胺-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:5000

    MAL-PEG-SVA-10K-100mg/500mg        马来酰亚胺-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:10K

    MAL-PEG-SVA-20K-100mg/500mg        马来酰亚胺-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:20K

    OPSS-PEG-SVA-3400-1g/500mg    OPSS-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:3400

    BOC-NH-PEG-SC-2K-1g/500mg     BOC-NH-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:2K

    BOC-NH-PEG-SC-5000-1g/5g/500mg   BOC-NH-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:5000

    BOC-NH-PEG-SVA-2000-1g/500mg       BOC-NH-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:2000

    修饰性PEG 聚乙二醇衍生物系列

    BOC-NH-PEG-SVA-5K-1g/500mg   BOC-NH-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:5K

    4arm-PEG-SAS-20K-1g   4arm-聚乙二醇-琥珀酰亚胺氨基琥珀酸酯  ,Mw:20K

    4arm-PEG-SC-20K-1g/5g        4arm-聚乙二醇-琥珀酰亚胺碳酸酯,Mw:20K

    MPEG-HZ-2000-1g 甲氧基聚乙二醇-酰肼,Mw:2000

    MPEG-HZ-5000-1g/5g    甲氧基聚乙二醇-酰肼,Mw:5000

    MPEG-HZ-10K-1g    甲氧基聚乙二醇-酰肼,Mw:10K

    MPEG-HZ-20K-1g/5g      甲氧基聚乙二醇-酰肼,Mw:20K

    HZ-PEG-HZ-2000-1g        酰肼-聚乙二醇-酰肼,Mw:2000

    HZ-PEG-HZ-3400-1g        酰肼-聚乙二醇-酰肼,Mw:3400

    修饰性PEG 聚乙二醇衍生物系列

    HZ-PEG-HZ-5000-1g/5g 酰肼-聚乙二醇-酰肼,Mw:5000

    HO-PEG-MAL-3400-1g/5g     HO-聚乙二醇-马来酰亚胺,Mw:3400

    HO-PEG-MAL-3400-500mg    HO-聚乙二醇-马来酰亚胺,Mw:3400

    HO-PEG-MAL-5000-1g   HO-聚乙二醇-马来酰亚胺,Mw:5000

    HO-PEG-VA-3400-1g/5g/500mg   HO-聚乙二醇-戊酸 ,Mw:3400

    FMOC-NH-PEG-OH-5000-1g/5g    FMOC-NH-聚乙二醇-OH,Mw:5000

    HO-PEG-MAL-2000-1g/500mg      HO-聚乙二醇-马来酰亚胺,Mw:2000

    mPEG-MAL-2000-1g       甲氧基聚乙二醇-马来酰亚胺,Mw:2000

    MPEG-MAL-5000-1g/5g         甲氧基聚乙二醇-马来酰亚胺,Mw:5000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-MAL-10K-1g/5g  甲氧基聚乙二醇-马来酰亚胺,Mw:10K

    MPEG-MAL-20K-1g/5g  甲氧基聚乙二醇-马来酰亚胺,Mw:20K

    MPEG-MAL-30K-1g/5g  甲氧基聚乙二醇-马来酰亚胺,Mw:30K

    MAL-PEG-MAL-3400-1g/5g   马来酰亚胺-聚乙二醇-马来酰亚胺,Mw:3400

    DSPE-PEG-MAL-3400-1g/5g  DSPE-聚乙二醇-马来酰亚胺,Mw:3400

    DSPE-PEG-MAL-3400-500mg         DSPE-聚乙二醇-马来酰亚胺,Mw:3400

    HO-PEG-MAL-3400-1g/100mg/500mg         HO-聚乙二醇-马来酰亚胺,Mw:3400

    修饰性PEG 聚乙二醇衍生物系列

    MAL-PEG-SVA-3400-1g/100mg/500mg        马来酰亚胺-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:3400

    MAL-PEG-SVA-5000-100mg/500mg      马来酰亚胺-聚乙二醇-琥珀酰亚胺戊酸酯,Mw:5000

    mPEG-MAL-1000-1g       甲氧基聚乙二醇-马来酰亚胺,Mw:1000

    MAL-PEG-MAL-2K-1g     马来酰亚胺-聚乙二醇-马来酰亚胺,Mw:2K

    MAL-PEG-MAL-5K-1g     马来酰亚胺-聚乙二醇-马来酰亚胺,Mw:5K

    MAL-PEG-MAL-10K-1g   马来酰亚胺-聚乙二醇-马来酰亚胺,Mw:10K

    DSPE-PEG-MAL-2000-500mg         DSPE-聚乙二醇-马来酰亚胺,Mw:2000

    4arm-PEG-MAL-20K-1g/5g    4arm-聚乙二醇-马来酰亚胺,Mw:20K

    mPEG-NPC-2000-1g/5g 甲氧基聚乙二醇-NPC,Mw:2000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-NPC-5000-1g/5g 甲氧基聚乙二醇-NPC,Mw:5000

    mPEG-NPC-10K-1g/5g   甲氧基聚乙二醇-NPC,Mw:10K

    mPEG-NPC-20K-1g/5g   甲氧基聚乙二醇-NPC,Mw:20K

    NPC-PEG-NPC-3400-1g/5g    NPC-聚乙二醇-NPC,Mw:3400

    mPEG-NPC-1000-1g       甲氧基聚乙二醇-NPC,Mw:1000

    NPC-PEG-NPC-2000-1g  NPC-聚乙二醇-NPC,Mw:2000

    BOC-NH-PEG-NPC-5000-1g/500mg      BOC-NH-聚乙二醇-NPC,Mw:5000

    MPEG-OPSS-5000-1g/5g        甲氧基聚乙二醇-OPSS,Mw:5000

    MPEG-OPSS-2000-1g      甲氧基聚乙二醇-OPSS,Mw:2000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-OPSS-10K-1g        甲氧基聚乙二醇-OPSS,Mw:10K

    MPEG-OPSS-20K-1g/5g 甲氧基聚乙二醇-OPSS,Mw:20K

    MPEG-OPSS-30K-1g/5g 甲氧基聚乙二醇-OPSS,Mw:30K

    OPSS-PEG-OPSS-2000-1g       OPSS-聚乙二醇-OPSS,Mw:2000

    OPSS-PEG-OPSS-3400-1g       OPSS-聚乙二醇-OPSS,Mw:3400

    MPEG-DSPE-5000-1g/5g        甲氧基聚乙二醇-DSPE,Mw:5000

    MPEG-DSPE-2000-1g      甲氧基聚乙二醇-DSPE,Mw:2000

    MPEG-SIL-5000-1g/5g   甲氧基聚乙二醇-硅烷,Mw:5000

    SIL-PEG-SIL-3400-1g       硅烷-聚乙二醇-硅烷,Mw:3400

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-SIL-1000-1g 甲氧基聚乙二醇-硅烷,Mw:1000

    MPEG-SIL-2000-1g 甲氧基聚乙二醇-硅烷,Mw:2000

    MPEG-SIL-10K-1g   甲氧基聚乙二醇-硅烷,Mw:10K

    MPEG-SIL-20K-1g/5g     甲氧基聚乙二醇-硅烷,Mw:20K

    MPEG-SIL-30K-1g   甲氧基聚乙二醇-硅烷,Mw:30K

    MPEG-SH-2000-1g/5g    甲氧基聚乙二醇-巯基,Mw:2000

    MPEG-SH-5000-1g/5g    甲氧基聚乙二醇-巯基,Mw:5000

    修饰性PEG 聚乙二醇衍生物系列

    MPEG-SH-10K-1g    甲氧基聚乙二醇-巯基,Mw:10K

    MPEG-SH-20K-1g/5g      甲氧基聚乙二醇-巯基,Mw:20K

    MPEG-SH-30K-1g/5g      甲氧基聚乙二醇-巯基,Mw:30K

    SH-PEG-SH-3400-1g/5g 巯基-聚乙二醇-巯基,Mw:3400

    4arm-PEG-SH-10K-1g/5g       4arm-聚乙二醇-巯基,Mw:10K

    MPEG-SH-1000-1g 甲氧基聚乙二醇-巯基,Mw:1000

    SH-PEG-SH-5K-1g/5g      巯基-聚乙二醇-巯基,Mw:5K

    修饰性PEG 聚乙二醇衍生物系列

    SH-PEG-SH-10K-1g/5g   巯基-聚乙二醇-巯基,Mw:10K

    4arm-PEG-SH-20K-1g/5g       4arm-聚乙二醇-巯基,Mw:20K

    VA-PEG-VA-5000-1g/5g 戊酸 -聚乙二醇-戊酸 ,Mw:5000

    VA-PEG-VA-20K-1g/5g   戊酸 -聚乙二醇-戊酸 ,Mw:20K

    修饰性PEG 聚乙二醇衍生物系列

    磷酸葡糖异构酶[酿酒酵母] Phosphoglucose isomerase (Saccharomyces cerevisiae) 货号:E-PGISC-50KU Megazyme中文站

    磷酸葡糖异构酶[酿酒酵母]

    英文名:Phosphoglucose isomerase (Saccharomyces cerevisiae)

    货号:E-PGISC-50KU

    规格:50000 units

    High purity Phosphoglucose isomerase (S. cerevisiae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

    EC 5.3.1.9

    From Saccharomyces cerevisiae. Electrophoretically homogeneous (MW 62,400). Single major band on isoelectric focusing (pI 6.1); minor band pI 6.9. 
    In 3.2M ammonium sulphate.

    Specific activity: ~ 650 U/mg of protein (25oC, pH 7.6) or ~ 850 U/mg of protein (40oC, pH 7.6).

    Stable at 4oC for > 4 years.

    Data booklets for each pack size are located in the Technical Resources tab.

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    Lumiprobe N-羟基琥珀酰亚胺酯染料 Dye NHS esters

    Lumiprobe N-羟基琥珀酰亚胺酯染料 Dye NHS esters

    Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学用染料和其它试剂。 产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。

    作为Lumiprobe代理,上海金畔生物生物可提供Lumiprobe的完整产品线,满足您的各种科研实验需求。产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规(市场上部分是分装,质量不能保证),质量保证,价格极具竞争力,而且我们的货期进需要3-5工作日,不需要像其他家攒单货期3-4周。

     Lumiprobe N-羟基琥珀酰亚胺酯染料 Dye NHS esters
    Cy3-N-羟基琥珀酰亚胺酯  Cyanine3 NHS ester

    Amine-reactive Cyanine3 dye NHS ester.

    Cyanine3 NHS ester
    Cy3.5-N-羟基琥珀酰亚胺酯 Cyanine3.5 NHS ester

    Cyanine3.5 dye NHS ester derivative.

    Cyanine3.5 NHS ester
    Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester

    Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

    Cyanine5 NHS ester
    Cy5.5-N-羟基琥珀酰亚胺酯 Cyanine5.5 NHS ester

    Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.

    Cyanine5.5 NHS ester
    Cy7-N-羟基琥珀酰亚胺酯 Cyanine7 NHS ester

    Amine reactive NHS ester of near infrared dye Cyanine7.

     Cyanine7 NHS ester
    Cy7.5-N-羟基琥珀酰亚胺酯 Cyanine7.5 NHS ester

    Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.

    Cyanine7.5 NHS ester
    6-ROX-N-羟基琥珀酰亚胺酯 ROX NHS ester, pure 6- isomer

    Pure isomer 6-ROX NHS ester for amine labeling.

    ROX NHS ester, pure 6- isomer
    磺酸基-Cy3-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine3 NHS ester

    Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

    Sulfo-Cyanine3 NHS ester
    磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester

    Water-soluble Cyanine 5 succinimide ester.

    Sulfo-Cyanine5 NHS ester
    磺酸基-Cy7-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine7 NHS ester

    Sulfo-Cyanine7 NHS ester is a water soluble, near infrared, amine-reactive dye.

    Sulfo-Cyanine7 NHS ester
    BDP FL N-羟基琥珀酰亚胺酯 BDP FL NHS ester

    Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.

    BDP FL NHS ester

    Lumiprobe N-羟基琥珀酰亚胺酯染料 Dye NHS esters

    货号

    中文名

    产品描述

    11420 BDP FL N-羟基琥珀酰亚胺酯 BDP FL NHS ester, 1 mg
    21420 BDP FL N-羟基琥珀酰亚胺酯 BDP FL NHS ester, 5 mg
    41420 BDP FL N-羟基琥珀酰亚胺酯 BDP FL NHS ester, 25 mg
    51420 BDP FL N-羟基琥珀酰亚胺酯 BDP FL NHS ester, 50 mg
    61420 BDP FL N-羟基琥珀酰亚胺酯 BDP FL NHS ester, 100 mg
    11020 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester, 1 mg
    21020 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester, 5 mg
    41020 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester, 25 mg
    51020 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester, 50 mg
    61020 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester, 100 mg
    12020 Cy3.5-N-羟基琥珀酰亚胺酯 Cyanine3.5 NHS ester, 1 mg
    22020 Cy3.5-N-羟基琥珀酰亚胺酯 Cyanine3.5 NHS ester, 5 mg
    42020 Cy3.5-N-羟基琥珀酰亚胺酯 Cyanine3.5 NHS ester, 25 mg
    52020 Cy3.5-N-羟基琥珀酰亚胺酯 Cyanine3.5 NHS ester, 50 mg
    62020 Cy3.5-N-羟基琥珀酰亚胺酯 Cyanine3.5 NHS ester, 100 mg
    13020 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester, 1 mg
    23020 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester, 5 mg
    43020 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester, 25 mg
    53020 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester, 50 mg
    63020 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester, 100 mg
    17020 Cy5.5-N-羟基琥珀酰亚胺酯 Cyanine5.5 NHS ester, 1 mg
    27020 Cy5.5-N-羟基琥珀酰亚胺酯 Cyanine5.5 NHS ester, 5 mg
    47020 Cy5.5-N-羟基琥珀酰亚胺酯 Cyanine5.5 NHS ester, 25 mg
    57020 Cy5.5-N-羟基琥珀酰亚胺酯 Cyanine5.5 NHS ester, 50 mg
    67020 Cy5.5-N-羟基琥珀酰亚胺酯 Cyanine5.5 NHS ester, 100 mg
    15020 Cy7-N-羟基琥珀酰亚胺酯 Cyanine7 NHS ester, 1 mg
    25020 Cy7-N-羟基琥珀酰亚胺酯 Cyanine7 NHS ester, 5 mg
    45020 Cy7-N-羟基琥珀酰亚胺酯 Cyanine7 NHS ester, 25 mg
    55020 Cy7-N-羟基琥珀酰亚胺酯 Cyanine7 NHS ester, 50 mg
    65020 Cy7-N-羟基琥珀酰亚胺酯 Cyanine7 NHS ester, 100 mg
    16020 Cy7.5-N-羟基琥珀酰亚胺酯 Cyanine7.5 NHS ester, 1 mg
    26020 Cy7.5-N-羟基琥珀酰亚胺酯 Cyanine7.5 NHS ester, 5 mg
    46020 Cy7.5-N-羟基琥珀酰亚胺酯 Cyanine7.5 NHS ester, 25 mg
    56020 Cy7.5-N-羟基琥珀酰亚胺酯 Cyanine7.5 NHS ester, 50 mg
    66020 Cy7.5-N-羟基琥珀酰亚胺酯 Cyanine7.5 NHS ester, 100 mg
    12220 6-ROX-N-羟基琥珀酰亚胺酯 ROX NHS ester, pure 6- isomer, 1 mg
    22220 6-ROX-N-羟基琥珀酰亚胺酯 ROX NHS ester, pure 6- isomer, 5 mg
    42220 6-ROX-N-羟基琥珀酰亚胺酯 ROX NHS ester, pure 6- isomer, 25 mg
    52220 6-ROX-N-羟基琥珀酰亚胺酯 ROX NHS ester, pure 6- isomer, 50 mg
    62220 6-ROX-N-羟基琥珀酰亚胺酯 ROX NHS ester, pure 6- isomer, 100 mg
    11320 磺酸基-Cy3-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine3 NHS ester, 1 mg
    21320 磺酸基-Cy3-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine3 NHS ester, 5 mg
    41320 磺酸基-Cy3-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine3 NHS ester, 25 mg
    51320 磺酸基-Cy3-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine3 NHS ester, 50 mg
    61320 磺酸基-Cy3-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine3 NHS ester, 100 mg
    13320 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester, 1 mg
    23320 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester, 5 mg
    43320 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester, 25 mg
    53320 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester, 50 mg
    63320 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester, 100 mg
    15320 磺酸基-Cy7-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine7 NHS ester, 1 mg
    25320 磺酸基-Cy7-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine7 NHS ester, 5 mg
    45320 磺酸基-Cy7-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine7 NHS ester, 25 mg
    55320 磺酸基-Cy7-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine7 NHS ester, 50 mg
    65320 磺酸基-Cy7-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine7 NHS ester, 100 mg