上海金畔生物科技有限公司TOP100生化试剂优惠活动

上海金畔生物科技有限公司TOP100生化试剂“七折又送礼”活动产品列表 活动时间:2015年3月20日—2014年6月30日

氨基酸类
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
G0524 Glutathione oxidized 1 g 250 175 进口分装
谷胱甘肽氧化型 5 g 1125 788
Reagent Grade
G0399 Glutathione reduced 5 g 121 85 进口分装
谷胱甘肽还原型 50 g 682 477
Reagent Grade
GB0235 Glycine 500 g 165 116 BBI
甘氨酸 5 kg 946 662
Molecular Biology Grade
AB0205 L-Arginine, free base 100 g 95 67 BBI
L-精氨酸 500 g 384 269
USP Grade
CB0132 L-Cysteine 100 g 124 87 BBI
L-半胱氨酸 500 g 504 353
USP Grade
GB0224 L-Glutamine 100 g 137 96 BBI
L-谷氨酰胺 500 g 418 293
BR Grade
蛋白类
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
A0903 Albumin, bovine, fraction V, cold alcohol isolation 1 g 299 209 BBI
牛血清白蛋白 5 g 1208 846
Molecular Biology Grade 10 g 2080 1456
S0492 Streptavidin from Streptomyces avidinii 1 mg 410 287 BBI
链霉亲和素
Biotech Grade
分离试剂
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
A1032 Acrylamide 250 g 121 85 BBI
丙烯酰胺 1 kg 424 297
Molecular Biology Grade
AB0014 Agarose B, Low EEO 50 g 287 201 BBI
琼脂糖 250 g 1303 912
Biotech Grade
AB0016H Agarose H 50 g 220 154 BBI
琼脂糖H 250 g 900 630
For Electrophoresis Use Grade
AB0013 Agarose M 50 g 185 130 BBI
琼脂糖M 250 g 750 525
For Electrophoresis Use Grade
AB1004 Agarose N 50 g 150 105 BBI
琼脂糖N 250 g 700 490
For Electrophoresis Use Grade
AB0015 Agarose, Low melting 5 g 169 118 BBI
低熔点琼脂糖 25 g 739 517
Biotech Grade
BB0025 Bis-acrylamide 50 g 90 63 BBI
N,N’-亚甲基双丙烯酰胺 250 g 380 266
Molecular Biology Grade
DB0160 Dextran sulfate, sodium salt 10 g 370 259 BBI
硫酸葡聚糖 50 g 1480 1036
Molecular Biology Grade 250 g 5180 3626
核酸及其衍生物
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
AB0001 ABA, (+) Abscisic acid 100 mg 240 168 BBI
脱落酸 500 mg 980 686
Plant Hormones Grade
AB0020 Adenosine 5′-triphosphate (ATP), disodium salt, trihydrate 5 g 145 102 BBI
5′-三磷酸腺苷二钠盐三水 25 g 667 467
Reagent Grade
D0056 dNTP mixture,10 mM 0.5 ml 25 18 BBI
dNTP mixture 溶液,10 mM
Molecular Biology Grade
D0055 dNTP mixture,25 mM 0.25 ml 32 22 BBI
dNTP mixture 溶液,25 mM
Molecular Biology Grade
SB2040 Green-DNA Dye 100 μl 120 84 BBI
核苷酸胶体染料 500 μl 478 335
Biotech Grade
ZB0748 trans-Zeatin 100 mg 508 356 BBI
反玉米素
Plant Hormones Grade
ZB0747 Zeatin 100 mg 508 356 BBI
玉米素
Plant Hormones Grade
抗生素
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
A0339 Ampicillin, sodium salt 5 g 100 70 进口分装
氨苄青霉素钠 25 g 416 291
USP Grade
CDJ469 Carbenicillin, disodium salt 1 g 233 163 BBI
羧苄青霉素二钠 5 g 760 532
USP Grade
CB1276 Cefotaxime, sodium salt 25 g 124 87 BBI
头孢噻肟钠 100 g 462 323
USP Grade
CB0118 Chloramphenicol 50 g 92 64 BBI
氯霉素 250 g 418 293
USP Grade
DB3456 DOX, doxorubicin hydrochloride 25 mg 315 221 BBI
盐酸阿霉素
Reagent Grade
GDJ958 G418 sulfate 100 mg 124 87 BBI
G418硫酸盐 1 g 414 290
USP Grade 5 g 1869 1308
GB0217 Gentamycin sulfate, Potency: >590 mcg/mg 1 g 110 77 BBI
硫酸庆大霉素 5 g 368 258
USP Grade 25 g 1560 1092
HD0230 Hygromycin B 250 mg 257 180 BBI
潮霉素B 1 g 858 601
USP Grade
KB0286 Kanamycin sulfate 5 g 102 71 BBI
硫酸卡那霉素 25 g 413 289
USP Grade 100 g 1500 1050
P0319 Polymyxin B sulfate 25 MU 2892 2024 BBI
多粘菌素 B 100 MU 9254 6478
USP Grade
PJ593 Puromycin, dihydrochloride 25 mg 756 529 BBI
嘌呤霉素盐酸盐
USP Grade
SB0901 Spectinomycin dihydrochloride 5 g 142 99 BBI
盐酸壮观霉素 25 g 603 422
USP Grade
S0382 Streptomycin sulfate 25 g 65 46 进口分装
硫酸链霉素 50 g 102 71
USP Grade 250 g 506 354
T0422 Tetracycline hydrochloride 10 g 77 54 进口分装
盐酸四环素 25 g 160 112
USP Grade 100 g 560 392
TB0950 Timentin 1 g 218 153 BBI
特泯菌
BC Grade
TF1129 Tunicamycin from Streptomyces lysosuperficus 5 mg 971 680 BBI
衣霉素
Antibiotic Grade
VB0983 Vancomycin hydrochloride 1 g 279 195 BBI
万古霉素盐酸盐
USP Grade
酶与辅酶
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
CLS002610 Cellulase 1 g 554 388 Worthington
纤维素酶
BC Grade
CB0320 Coenzyme A, free acid, hydrate (COA) 100 mg 399 279 BBI
辅酶 A
Reagent Grade
CLS004188 Collagenase, Type 4 1 g 2301 1611 Worthington
胶原酶 4型 >160 U/mg
CLS004196 Collagenase, Type1 1 g 2301 1611 Worthington
胶原酶1型 >125 U/mg
CLS004176 Collagenase, Type2 1 g 2301 1611 Worthington
胶原酶2型 >125 U/mg
DD0099 DNase I, from Bovine Pancreas 250 mg 122 85 BBI
脱氧核糖核酸酶 1 g 489 342
Molecular Biology Grade 5 g 2231 1562
L0253 Lactate dehydrogenase 25 KU 1620 1134 BBI
乳酸脱氢酶悬浮液
BC Grade
LJ580 Leupeptin 5 mg 490 343 BBI
亮抑酶肽 25 mg 1518 1063
BC Grade
SD9001 Lysostaphin 1 mg 546 382 BBI
溶葡球菌酶
BC Grade
LDB0308 Lysozyme 1 g 26 18 BBI
溶菌酶 5 g 121 85
Molecular Biology Grade 100 g 1798 1259
NB0642 NADH disodium salt, trihydrate, reduced 1 g 313 219 BBI
还原型辅酶 I(钠盐) 5 g 1327 929
BC Grade
N0760 NADP 250 mg 485 340 BBI
氧化型辅酶 II 1 g 1515 1061
BC Grade 5 g 5310 3717
Y4433000 NADPH 100 mg 832 582 BBI
烟酰胺腺嘌呤二核苷酸磷酸四钠盐还原型
BC Grade
PB0691 Peroxidase, horseradish Rz >3.0 25 mg 208 146 BBI
辣根过氧化物酶 100 mg 764 535
BC Grade
PLS003789 Polyphenol Oxidase from Mushrooms 25 KU 472 330 Worthington
多酚氧化酶
BC Grade
PB0451 Proteinase K from Tritirachium album 50 mg 163 114 BBI
蛋白酶 K 250 mg 777 544
BC Grade
RB0478 Ribonuclease inhibitor from Human Placenta 1 KU 155 109 BBI
核糖核酸酶抑制剂 5 KU 670 469
Molecular Biology Grade
RB0473 RNase A from bovine pancreas 25 mg 63 44 BBI
核糖核酸酶 A 100 mg 210 147
Molecular Biology Grade 500 mg 735 515
T0458 Trypsin 1:250 fromPorcine pancreas 10 g 143 100 进口分装
胰蛋白酶1:250 50 g 567 397
USP Grade 250 g 2891 2024
染色剂
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
BD0159 Bromocresol green, free acid 5 g 79 55 BBI
溴甲酚绿 25 g 330 231
Reagent Grade
B0449 Bromophenol blue 5 g 84 59 进口分装
溴酚蓝 25 g 336 235
BS Grade 100 g 1314 920
CB0038 Coomassie brilliant blue G-250 25 g 179 125 BBI
考马士亮兰 G-250 100 g 683 478
BS Grade
CB0037 Coomassie brilliant blue R-250 5 g 44 31 BBI
考马士亮兰 R-250 25 g 176 123
BS Grade
D6584 DAPI dihydrochloride 10 mg 840 588 BBI
4,6-二脒基-2-苯基吲哚二盐酸盐
BS Grade
EX328 EB, Ethidium bromide stock solution, 10 mg/ml 5 ml 88 62 BBI
溴化乙锭溶液,10 mg/ml
Biotech Grade
EB0195 EB, Ethidium bromide 1 g 130 91 BBI
溴化乙锭 5 g 374 262
BS Grade 25 g 1683 1178
P1112 Propidium iodide 20 mg 242 169 BBI
碘化丙啶 100 mg 1008 706
BC Grade
T0793 Thiazolyl blue tetrazolium bromide 250 mg 107 75 进口分装
噻唑兰 1 g 244 171
BR Grade 5 g 1023 716
T0759 TMB dihydrochloride 250 mg 189 132 进口分装
3,3′,5,5′-四甲基联苯胺盐酸盐二水 1g 447 313
Biotech Grade 5 g 1873 1311
生物缓冲液
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
BB0079 Bis-Tris 25 g 54 38 BBI
双(2-羟乙基)氨基(三羟甲基)甲烷 100 g 187 131
Reagent Grade
CD0110 CHAPS, 3-[(3-Chloamidopropyl)dimethyl- ammonium]-1- propanesulfonate 1 g 105 74 BBI
3-[3-(胆酰胺丙基)二甲氨基]-1-丙磺酸 5 g 420 294
Reagent Grade
HB0264 HEPES, free acid 50 g 53 37 BBI
N-(2-羟乙基)哌嗪- N’-2磺酸 250 g 210 147
Reagent Grade 1 kg 630 441
MB0341 MES, free acid, monohydrate 25 g 63 44 BBI
2-(N-吗啉代)乙烷磺酸一水 100 g 189 132
Molecular Biology Grade 500 g 756 529
MB0360 MOPS, free acid 100 g 147 103 BBI
3-(N-吗啡啉)丙磺酸 500 g 714 500
Molecular Biology Grade
TDB0546 Tricine buffer, free acid 100 g 210 147 BBI
N-[三(羟甲基)甲基]甘氨酸 500 g 840 588
BC Grade
T0234 Tris hydrochloride 100 g 142 99 进口分装
三(羟甲基)氨基甲烷盐酸盐 500 g 593 415
Molecular Biology Grade
TB0194 Tris 500 g 103 72 BBI
三(羟甲基)氨基甲烷 5 kg 927 649
Molecular Biology Grade
碳水化合物
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
MB4950 D-(+) Maltose, monohydrate 100 g 120 84 BBI
麦芽糖一水 500 g 470 329
BC Grade
GB0215 D(+)Galactose 25 g 84 59 BBI
D(+)半乳糖 100 g 231 162
BC Grade 500 g 1048 734
TB0966 D-(+)-Trehalose, dihydrate from Saccharomyces cerevisiae 25 g 168 118 BBI
D-海藻糖二水 100 g 598 419
BC Grade
GB0219 D-Glucose 1 kg 97 68 BBI
D-葡萄糖
Molecular Biology Grade
G0188 D-Glucose 500 g 80 56 进口分装
D-葡萄糖 5 kg 630 441
Molecular Biology Grade
S0691 D-Sorbitol 500 g 77 54 进口分装
D-山梨醇
Biotech Grade
DB0058 DTT 5 g 170 119 BBI
二硫苏糖醇 25 g 756 529
Molecular Biology Grade
IB0168 IPTG Dioxane Free 1 g 50 35 BBI
异丙基-b-D-硫代半乳糖苷 5 g 184 129
Molecular Biology Grade 25 g 825 578
AB0071L L(+)-Arabinose 25 g 178 125 BBI
L(+)-阿拉伯糖 100 g 630 441
Reagent Grade
RJ392 Raffinose, pentahydrate 25 g 326 228 进口分装
蜜三糖五水 100 g 1155 809
BR Grade
S0335 Sucrose 250 g 68 48 进口分装
蔗糖 5 kg 878 615
Molecular Biology Grade
BB0083 X-Gal 100 mg 82 57 BBI
5-溴-4-氯-3吲哚基-D半乳糖苷 1 g 292 204
Molecular Biology Grade 5 g 1321 925
BB0085 X-GLUC 25 mg 200 140 BBI
5-溴-4-氯-3-吲哚基-beta-D-葡糖苷酸环己胺盐 100 mg 700 490
Molecular Biology Grade
其他
目录编号 产品名称/等级 包装规格 目录价 七折 品牌
FB1555 5-FOA, 5-Fluoroorotic acid 1 g 174 122 BBI
5-氟乳清酸 5 g 628 440
Reagent Grade
AD0002 ABTS, diammonium salt 1 g 193 135 BBI
2,2-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)二铵盐 5 g 945 662
Reagent Grade
AB1111 Acetosyringone 1 g 292 204 BBI
乙酰丁香酮
Reagent Grade
A0191 Ammonium sulfate 500 g 82 57 进口分装
硫酸铵 5 kg 690 483
Molecular Biology Grade
A0486 APS, Ammonium persulfate 25 g 52 36 进口分装
过硫酸铵 100 g 137 96
ACS Grade
B714229 Basta, 10% solution 100 ml 484 339 BBI
除草剂, 10%溶液
Plant Hormones Grade
BB0072 BCIP, P-toluidine salt 100 mg 250 175 BBI
5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐 500 mg 830 581
Molecular Biology Grade
CDB0054 Cesium chloride 50 g 168 118 BBI
氯化铯 250 g 731 512
Molecular Biology Grade
CD0122 Cholesterol 50 g 141 99 BBI
胆固醇 250 g 424 297
Reagent Grade
CB0108 CTAB, Cetyltrimethylammonium bromide 100 g 102 71 BBI
十六烷基-三甲基-溴化铵 500 g 423 296
Reagent Grade
BB0078 D-Biotin 1 g 53 37 BBI
D-生物素 5 g 210 147
USP Grade
DD0150 Deoxycholic acid, sodium salt 50 g 260 182 BBI
脱氧胆酸钠盐 250 g 941 659
Reagent Grade
DB0154 DEPC, diethypyrocarbonate 5 ml 83 58 BBI
焦碳酸二乙酯 25 ml 315 221
Molecular Biology Grade 100 ml 1260 882
D0231 Dimethyl sulfoxide (DMSO) 500 ml 197 138 进口分装
二甲亚砜
Molecular Biology Grade
DB0339 DNA & RNase AwayTM 200 ml 231 162 BBI
去核酸酶试剂
Molecular Biology Grade
E0105 EDTA, disodium salt, dihydrate 100 g 37 26 进口分装
乙二胺四乙酸二钠盐二水 500 g 137 96
Reagent Grade
EB0107 EDTA, free acid 500 g 118 83 BBI
乙二胺四乙酸
Reagent Grade
ED0077 EGTA, egtazic acid, Glycol ether diamine tetraacetic acid 25 g 180 126 BBI
乙二醇双(2-氨基乙基醚)四乙酸 100 g 714 500
BC Grade
FB0211 Formamide deionized 100 ml 90 63 BBI
去离子甲酰胺 500 ml 192 134
Molecular Biology Grade
F0314 Formamide 100 ml 61 43 进口分装
甲酰胺 500 ml 231 162
Molecular Biology Grade
G9764 Gelatin 100 g 103 72 BBI
明胶 500 g 450 315
CP Grade
G0854 Glycerol 100 ml 35 25 进口分装
甘油 500 ml 137 96
Molecular Biology Grade
GB0486 Glycero-phosphate, disodium salt 25 g 44 31 BBI
甘油磷酸钠 100 g 105 74
Reagent Grade
GB0242 Guanidine hydrochloride 100 g 126 88 BBI
盐酸胍 500 g 400 280
Molecular Biology Grade
GE424 Guanidine hydrochloride, low ASH 100 g 55 39 BBI
盐酸胍 500 g 221 155
Tech Grade 5 kg 1971 1380
GB0244 Guanidine thiocyanate 100 g 168 118 BBI
硫氰酸胍 500 g 560 392
Molecular Biology Grade
HB3251 Heparin sodium from Porcine Intestinal 1 g 360 252 BBI
肝素钠盐
BC Grade
IB0277 Imidazole 100 g 100 70 BBI
咪唑 500 g 300 210
Molecular Biology Grade
MB0328 Magnesium chloride, hexahydrate 500 g 120 84 BBI
氯化镁六水 5 kg 966 676
Molecular Biology Grade
NDB0379 Nitrotetrazolium blue chloride 250 mg 61 43 BBI
氯化硝基四氮唑蓝 1 g 242 169
BS Grade 5 g 1155 809
NB0669 NON-Fat Powdered Milk 250 g 82 57 BBI
脱脂奶粉
Commercial Grade
NE109 Nonidet® P-40 (NP-40) 100 ml 134 94 进口分装
诺纳德® P-40 500 ml 584 409
Molecular Biology Grade
SD0301 Paraffin oil, light fraction 100 ml 48 34 BBI
石蜡油 500 ml 190 133
PCR Grade
PT1781 PEG 400 500 g 53 37 BBI
聚乙二醇400
Reagent Grade
PB0432 PEG 6000 500 g 90 63 BBI
聚乙二醇6000
Molecular Biology Grade
P0159 PEG 8000 500 g 163 114 进口分装
聚乙二醇8000
Molecular Biology Grade
PB0425 PMSF 5 g 208 146 BBI
苯甲基磺酰 25 g 880 616
BC Grade
PB0445 Potassium phosphate, monobasic, anhydrous 500 g 84 59 BBI
无水磷酸二氢钾
Molecular Biology Grade
S0227 SDS 100 g 73 51 进口分装
十二烷基硫酸钠 500 g 346 242
Molecular Biology Grade
S0865 Sodium bicarbonate 500 g 102 71 进口分装
碳酸氢钠
ACS Grade
SB0476 Sodium chloride 1 kg 68 48 BBI
氯化钠 5 kg 281 197
Molecular Biology Grade
CB0035 Sodium citrate, trisodium salt, dehydrate 500 g 65 46 BBI
柠檬酸三钠二水 5 kg 594 416
Biotech Grade
S0404 Sodium phosphate, dibasic, anhydrous 100 g 53 37 BBI
无水磷酸氢二钠 500 g 210 147
Molecular Biology Grade
T0694 Triton X-100 100 ml 43 30 进口分装
曲拉通X-100 500 ml 116 81
Molecular Biology Grade
T0777 Tween-20 500 ml 119 83 进口分装
吐温-20
Reagent Grade
UB0148 Urea 500 g 71 50 BBI
尿素 2 kg 252 176
Molecular Biology Grade
DD1005 Water-DEPC treate 500 ml 76 53 BBI
DEPC水
Molecular Biology Grade
Z706211 Zeocin 100 mg 535 375 BBI
抗菌防腐剂
BC Grade

三菱卡尔费休试剂-库仑法AKX/AXI/CXU 【通用型】阳极液AXI

三菱卡尔费休试剂-库仑法AKX/AXI/CXU
三菱卡尔费休试剂-库仑法AKX/AXI/CXU 【通用型】阳极液AXI
经销商促销活动(2016/12/19起至2017/1/23)
库伦法阴极液阳极液容量法滴定液脱水溶剂。可以与三菱化学、瑞士万通、京都电子、梅特勒等品牌的卡氏水分仪配套使用。【通用型】阳极液AXI/阴极液CXU,样品为:油类、锂电池电解液、有机溶剂、无机化学品、石油产品等除醛酮外的大部分样品。【醛酮型】阳极液AKX/阴极液CXU,为酮类等专用试剂。

【详细说明】

三菱卡尔费休试剂卡尔费休试剂-经销商促销活动

(促销活动有效期2016/12/19起至2017/1/23)

上海金畔生物科技有限公司提供三菱卡尔费休试剂-库仑法AKX/AXI/CXU,现货,价格优(促销活动有效期2016/12/19起至2017/1/23),可与三菱化学、瑞士万通、京都电子、梅特勒等品牌的卡氏水分仪配套使用。

优势产品:库仑法阳极液AQUAMICRON ®AXI/AKX

库仑法阴极液AQUAMICRON ®CXU

【通用型】阳极液AXI/阴极液CXU,样品为油类(压缩机油/变压器油/绝缘油等)、锂电池电解液、有机溶剂、无机化学品、石油产品等除醛酮外的大部分样品。

【醛酮型】阳极液AKX/阴极液CXU,样品为酮类、硅油、低羟基酸、某些醛类(芳香族化合物)等专用试剂。

货号 品名 包装 主要溶剂 目录价(RMB)/瓶 库存
621-01755 【通用型】阳极液AXI 500ml 甲醇,碳酸丙烯酯,不含CHCl3和吡啶 1000 现货
605-07591 【醛酮型】阳极液AKX 500ml 碳酸丙烯酯,二甘醇-甲醚,不含CHCl3,含有4-二甲胺基吡啶 1050 现货
602-07501 阴极液CXU,与AXI/AKX配套使用 5ml*10 甲醇,不含吡啶 1050 现货

乙酸[GK法]检测试剂盒 Acetic Acid GK Assay Kit (Analyser Format) 货号:K-ACETGK Megazyme中文站

乙酸[GK法]检测试剂盒

英文名:Acetic Acid GK Assay Kit (Analyser Format)

货号:K-ACETGK

规格:110 mL of prepared reagent (e.g. 500 assays of 0.22 mL)

The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.

As part of Megazyme’s overall commitment to providing the highest quality products, we have developed this acetic acid kit that provides a specific and rapid assay for use with auto-analysers. The kit assay is based on the conversion of NAD+ to NADH and therefore provides a positive reaction (increase in absorbance) which offers a more robust assay.

The reagents, as supplied, are stable for a minimum of 2 years and the prepared reagents are stable for a minimum of 1 week (on-board stability). In addition, the prepared reagents can be stored frozen for longer term stability

Q1. Is the acetic acid kit specific for acetate?

Propionate may react more slowly than acetate.

Q2. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q3. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q4. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q5. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q6. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q7. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q8. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q9. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q10. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q11. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q12. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

NS神经细胞基础培养基


产品编号 产品名称 产品规格 产品等级 产品价格
148-09615 NS Basal Medium 500ml 用于细胞培养
146-09351 NS Supplement(×50) 10ml 用于细胞培养
073-05391 200mmol/l L-Glutamin Solution(×100) 100ml 用于细胞培养
141-08941 N2 Supplement with Transferrin(Holo) 5ml 用于细胞培养
141-09041 N2 Supplement with Transferrin(Apo) 5ml 用于细胞培养

NS基础培养基/NS SupplementNS神经细胞基础培养基

用于神经细胞/神经干细胞培养


  NS基础培养基是、最适合用于神经细胞培养的基础培养基。NS Supplement是用于神经细胞培养的无血清Supplement(含有维生素A乙酸酯Retinyl Acetate)。适用于从大鼠海马分离出的神经细胞培养、神经干细胞等的培养。请将NS基础培养基和NS Supplement混合后使用



◆来自大鼠海的初代神经细胞培养


  ●Poly-L-lysine涂层板上培养了从大鼠胎儿(E19)海马分离出来的神经细胞。
  观察到培养6天后的细胞形态、培养21天后的神经细胞标记(Map2(a+b))以及胶质细胞标记(GFAP)。



培养6天后



                     NS基础培养基/NSSupplement                                          同等同行培养基/Supplement 

NS神经细胞基础培养基

( 培养基组成 )
 NS基础培养基 + 2% NS Supplement + 0.5mmol/l L-谷氨酰胺
 ( 细胞播种数 )
 13,000cells/well(96孔细胞培养板)



培养21天后



NS神经细胞基础培养基




来自大鼠大的初代神经细胞培养


  ●在Poly-L-lysine涂层板上培养了从大鼠胎儿(E17)大脑皮质分离出来的神经细胞。
  观察到培养8天后的细胞形态、培养14天后的神经细胞标记(Map2(a+b))以及胶质细胞标记(GFAP)。



培养8天后



NS神经细胞基础培养基 

( 培养基组成 )
 NS基础培养基 + 2% NS Supplement + 0.5mmol/l L-谷氨酰胺
( 细胞播种数)
 60,000cells/well(96孔细胞培养板)



培养14天后



NS神经细胞基础培养基


 

 

 

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作用于高支链和不可溶解纤维质纤维素内切酶唯一检测底物 Cellazyme T – 1000 Tablets 货号:T-CTZ-1000T Megazyme中文站

作用于高支链和不可溶解纤维质纤维素内切酶唯一检测底物

英文名:Cellazyme T – 1000 Tablets

货号:T-CTZ-1000T

规格:1000 Tablets

High purity dyed and crosslinked Cellazyme T tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis. 

A unique substrate for the measurement of endo-cellulases active on highly branched and insoluble cellulosic substrates. Containing AZCL-Xyloglucan. Highly sensitive substrate.

作用于高支链和不可溶解纤维质纤维素内切酶唯一检测底物

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Pall颇尔HT Tuffryn ® 膜的Acrodisc ® 针头过滤器

Pall颇尔HT Tuffryn ® 膜的Acrodisc ® 针头过滤器

【简单介绍】

适用于极低蛋白含量的生物溶液。
.极低蛋白吸附膜。
.通过y 射线灭菌,消除可能因EtO 残留物而导致的污染。
.高温和生物安全性经过验证。

【详细说明】

  • 应用
    .灭菌产品适合于过滤那些极低蛋白含量的生物溶液。
    .未灭菌产品可以用来准备HPLC 或FPLC 生物样品。
    . Acro®50A 过滤器的独特设计为标准47mm 可反复用过滤器的有效过滤面积的两倍,处理量和流速也加倍。
    订购信息
    HT tuffryn膜的acrodisc针头过滤器
    产品编号
    说明
    包装
    4454
    0.2µm 13mm 未灭菌
    75个/包装
    4192
    0.2µm 25mm 未灭菌
    50个/包装
    4184
    0.45µm 25mm 未灭菌
    50个/包装
    4496
    0.2µm 25mm 未灭菌
    75个/包装 300/盒
    4214
    0.2µm 25mm 未灭菌
    1000个/包装
    4497
    0.45µm 25mm 未灭菌
    75个/包装 300/盒
    4784
    0.45µm 25mm 未灭菌
    1000个/包装
    HT tuffryn膜的acro50A滤器
    产品编号
    说明
    包装
    4260
    0.65/0.2µm 50mm 灭菌
    18个/包装
    4262
    0.65/0.45µm 50mm 灭菌
    18个/包装
    相关的产品
    HT tuffryn膜的囊式滤器
    HT tuffryn圆盘过滤膜片

HT Tuffryn ® 膜的Acrodisc ® 针头过滤器
性能经过验证,用于关键应用

说明
.适用于极低蛋白含量的生物溶液。
.极低蛋白吸附膜。
.通过y 射线灭菌,消除可能因EtO 残留物而导致的污染。
.高温和生物安全性经过验证。
.非灭菌包装产品可以用于生物试剂分析的准备工作。
. AcroR50A 滤器用于大体积样品。