D-果糖/D-葡萄糖检测试剂盒 D-Fructose/D-Glucose Assay Kit 货号:K-FRUGL Megazyme中文站

D-果糖/D-葡萄糖检测试剂盒

英文名:D-Fructose/D-Glucose Assay Kit

货号:K-FRUGL

规格:110 assays (manual) / 1100 assays (micropl

分析物意义:非常常见的食物糖分,如从高果糖玉米种提炼的糖浆增补剂 

Megazyme检测试剂盒优点:反应时间快,可选择简单的版式,适用于手工和自动分析仪检测。试剂稳定 

D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

UV-method for the determination of D-Fructose and D-Glucose 
in foodstuffs, beverages and other materials

Principle:
                        (hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

                         (hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP

          (glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

    (phosphoglucose isomerase)
(4) F-6-P             ↔             G-6-P

Kit size:                            110 assays (manual) / 1100 (microplate)
                                          / 1100 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 13 min
Detection limit:                 0.66 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods,
bread, bakery products, candies, desserts, confectionery, ice-cream,
fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals,
paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. The calibration curve in the K-FRUGL data booklet is based on using a 4.6 mm pathlength cuvette. The cuvettes I am using have a 10 mm path-length. Do I have to have the 4.6 mm path-length cuvettes or can I use 10 mm path-length cuvettes?

If a spectrophotometer and standard 10 mm path-length cuvettes are being used, then the assay should be performed as described in the data booklet for either of the “Manual Format” methods (individual values of fructose and glucose or total sugars; glucose plus fructose).

These “Manual Format” methods do not require the use of a standard calibration curve and results should be obtained from the calculations as described for the appropriate method used.  Alternatively, results can be processed using the MegaCalc application which can be found where the product is located on the Megazyme website.  This application processes results from simple entry of raw data absorbance values.  The standard calibration curve is purely for use with auto-analyser instruments (e.g. Konelab) where the path-lengths vary from 10 mm and therefore do not permit use of the calculations in the data booklet or the MegaCalc application.

Q5. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q6. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q7. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q8. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q9. Can oligosaccharides or polysaccharides be measured using the kit assay?

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

Q10. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q11. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q12. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q13. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q14. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q15. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q16. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q17. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

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英国雷尼绍三坐标测头系统 MCP TP20 TP200  PH10T MCR20 SCR200  光栅尺 读数头

RENISHAW机床测头系统 OMP60 RMP60 LP2测头 控制器

YAMAMOTO日本山本科学硬度块

日本KORI 古里硬度计

日本ACCRETECH东京精密测针

日本大菱水平仪AS401  偏摆仪

日本RSK水平仪 740B

TECLOCK 得乐量表/硬度计  TM-35-01

日本小寺KOD水平 角度仪

MARUI日本丸井角度尺  LM-90

日本ASKER C型硬度计

医药化妆品相关试剂


产品编号 产品名称 产品规格 产品等级 产品价格

医药化妆品相关试剂医药化妆品相关试剂




医药化妆品相关试剂◆D(+)-五水棉子糖


CAS.No:17629-30-0

分子量:594.51

应用:冷冻保护剂、干燥保护剂、生物体材料保存稳定剂,有机合成原料;



医药化妆品相关试剂乳酸钠溶液


CAS.No72-17-3(DL体)、867-56-1(L体)

分子量:112.06


DL-50

(DL体 50%水溶液) 日本药局方外   医药品规格

DL-72

(DL体 72%水溶液) 日本药局方外   医药品规格

L-50

( L体 50%水溶液) 日本药局方

L-72

( L体 72%水溶液) 日本药局方

 

应用:医药品原料、输液原料

 

医药化妆品相关试剂2,6-二叔丁基对甲酚 (BHT)


CAS.No: 128-37-0

分子量: 220.36


物理性质:

外观

形状:结晶~结晶性粉末(含小块)
颜色:白色

溶解性

难溶
其他可溶于乙醇


相关法规·安全性

化审法

3-540(第三种监视化学物质)

安卫法

公布化学物质

致突变性

DNA抑制人淋巴球 20μmol/L

慢性致死实验 经口 大鼠5460mg/kg连续10周投放

经口毒性(LD50)

大鼠 890mg/kg

应用:食品添加剂

 

医药化妆品相关试剂苯甲酸钠(粉末)


CAS.No: 532-32-1

分子量: 144.10


物理性质:

外观

形状:粉末
  颜色:白色

溶解性

水 :可溶
  其他 :可溶于乙醇


相关法规·安全性

化审法

3-1293,3-1272

安卫法

公布化学物质

致突变性

在大鼠显性致死实验(遗传突变性实验)和大鼠骨髓细胞中的染色体异常试验(用体细胞进行有机活体突变性实验)呈阴性。(JETOC)

经口毒性(LD50)

大鼠 4,070mg/kg (RTECS)
大鼠 3,140mg/kg (SIDS)
小鼠 1,600mg/kg (RTECS)

应用:食品添加剂




医药化妆品相关试剂二氧化钛(局方品)


CAS.No: 13463-67-7

分子量: 79.90


物理性质:

外观

形状:粉末
颜色:白色

熔点

1,855℃

溶解性

水 :不溶
其他 :可溶于硫酸、强碱;不溶于盐酸、硝酸


相关法规·安全性

化审法

1-558

安卫法

公布化学物质

致突变性

Micronucleus test – 腹膜 小鼠 3 gram/kg/3D-C
Micronucleus test – 卵巢 仓鼠 5 μmol/L
DNA inhibition – 肺 仓鼠 500mg/L

药事法

日本药局方

 


医药化妆品相关试剂环己酮二甲基缩醛


CAS.No: 933-40-4

分子量: 144.21


物理性质:

外观

形状:液体
  颜色:无色~略黄色

溶解性

水 :难溶
  其他 :易溶于有机溶剂


相关法规·安全性:

消防法

危险物第四类第二石油类(非水溶性液体)

化审法

3-2257

安卫法

公布化学物质

应用:合成原料

 

 

 

医药化妆品相关试剂2-溴-1,1-二乙氧基乙烷


CAS.No[2-Bromo-1,1-diethoxyethane]: 2032-35-1


物理性质:

外观

形状:液体
颜色:无色~微黄色透明

溶解性

水:不溶
  其他:可溶于有机溶剂


相关法规·安全性:

消防法

危险物第四类第二石油类(非水溶性液体)

有毒物质及有害物质监控法

化审法

[2-Bromo-1,1-diethoxyethane]: 2-2467

安卫法

[2-Bromo-1,1-diethoxyethane]:   2-(8)-326,2-(8)-324

致突变性

阴性

应用:合成原料

 

BLUE-dextran 蓝色葡聚糖

上海金畔生物提供BLUE-dextran 蓝色葡聚糖

上海金畔生物提供蓝色葡聚糖有8个分子量。蓝色葡聚糖通过来源于肠系膜明串珠菌与汽巴克隆蓝色 F3GA反应而得到葡聚糖的片段合成而制得。

相关产品:

货号 英文品名 品名 分子量 规格
BD5 Blue dextran 5 蓝色葡聚糖5 5000 1g
BD10 Blue dextran 10 蓝色葡聚糖10 10000 1g
BD20 Blue dextran 20 蓝色葡聚糖20 20000 1g
BD5 Blue dextran 5 蓝色葡聚糖5 5000 10g
BD10 Blue dextran 10 蓝色葡聚糖10 10000 10g
BD20 Blue dextran 20 蓝色葡聚糖20 20000 10g
BD40 Blue dextran 40 蓝色葡聚糖40 40000 1g
BD70 Blue dextran 70 蓝色葡聚糖70 70000 1g
BD110 Blue dextran 110 蓝色葡聚糖110 110000 1g
BD500 Blue dextran 500 蓝色葡聚糖500 500000 1g
BD2000 Blue dextran 2000 蓝色葡聚糖2000 2000000 1g
BD40 Blue dextran 40 蓝色葡聚糖40 40000 10g
BD70 Blue dextran 70 蓝色葡聚糖70 70000 10g
BD110 Blue dextran 110 蓝色葡聚糖110 110000 10g
BD500 Blue dextran 500 蓝色葡聚糖500 500000 10g
BD2000 Blue dextran 2000 蓝色葡聚糖2000 2000000 10g

更多产品,更多优惠,请联系我们!

上海金畔生物科技有限公司

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