Amresco 原型辅酶Ⅰ NADH

Amresco 原型辅酶Ⅰ NADH 

还原型辅酶Ⅰ,NADH,烟酰胺腺嘌呤二核苷酸,还原态,N指烟酰胺,A指腺嘌呤,D是二核苷酸。
用于糖酵解和细胞呼吸作用中的柠檬酸循环。
NAD+ 则是氧化态。
葡萄糖代谢时直接经代谢所产生的ATP是十分的少的,而代谢产生的NADH或FADH2经由一个电子传递与氧化磷酸反应可产生大量的ATP。
烟酰胺腺嘌呤二核苷酸(氧化态)NAD+
烟酰胺腺嘌呤二核苷酸(还原态)NADH
烟酰胺腺嘌呤二核苷酸磷酸(还原态) NADPH
烟酰胺腺嘌呤二核苷酸磷酸(氧化态) NADP+
NAD+ + H+ + 2e- = NADH
NADP+ + H+ + 2e- = NADPH 他们都是辅酶,用来实现电子传递。
基本上涉及到氧化还原的反应都用得到,比如呼吸作用,光合作用,等等氨会抑制呼吸过程中的电子传递系统,尤其是NADH。
1分子NADH+H+在氧化磷酸化过程中生成2.5分子的ATP。
NAD+分子中的功能部分是烟酰胺换。其共振结构式:
“4-5双键振至5-6双键;6-7双键振至7-8双键;4号碳为碳正离子;7号氮为双电子原子”
Amresco 0384-1G 1G

96书机架隔热套(高沸点溶剂用)|柴田科技有限公司-环境检测设备、科学仪器的制造销售

产品详细

科学仪器

96书机架隔热套(高沸点溶剂用)

96书机架隔热套(高沸点溶剂用)|柴田科技有限公司-环境检测设备、科学仪器的制造销售

  • 96书机架隔热套(高沸点溶剂用)|柴田科技有限公司-环境检测设备、科学仪器的制造销售

商品代码其他情报(式样)

这个产品比较表中追加
产品照片 96书机架隔热套(高沸点溶剂用)|柴田科技有限公司-环境检测设备、科学仪器的制造销售
商品代码 F 34504 – 1923
型式
价格(不含税) 170万日元。

上海金畔生物科技有限公司

” 709%2F%3Fc%3D35 “> 709%2F%3Fc%3D35

iP-TEC® 保温运输箱(长距离)


产品编号 产品名称 产品规格 产品等级 产品价格
WEB28482  iP-TEC premium box-V8.5 (box only)
 iP-TEC 精装版保温运输箱 V8.5(仅箱子)
1 pc
WEB28481  iP-TEC premium box-V8.5 set ( box,t heat storage-36 5pcs)
 iP-TEC精装版保温运输箱 V8.5 套装 (箱子,36-蓄热板5块)
1 set
WEB28486  iP-TEC premium box-V8.5 set ( box,t heat storage-24 5pcs)
 iP-TEC精装版保温运输箱 V8.5 套装 (箱子,24-蓄热板5块)
1 set
WEB28460  iP-TEC premium box-V19 (box only)
 iP-TEC精装版保温运输箱 V19 (仅箱子)
1 pc
WEB28462  iP-TEC premium box-V19set( box,t heat storage-36 6pcs)
 iP-TEC精装版保温运输箱 V19套装(箱子,36-蓄热板6块)
1 set
WEB28484  iP-TEC premium box-V19 set ( box,t heat storage-24 6pcs)
 iP-TEC精装版保温运输箱 V19 套装(箱子,24-蓄热板6块)
1 set
WEB28463  iP-TEC standard box-X13 (box only)
 iP-TEC标准版保温运输箱 X13 (仅箱子)
1 pc
WEB28465  iP-TEC standard box-X13set ( box,t heat storage-36 6pcs) 
iP-TEC标准版保温运输箱 X13 套装(箱子,36-蓄热板6块)
1 set
WEB28485  iP-TEC standard box-X13set ( box,t heat storage-36 6pcs) 
 iP-TEC标准版保温运输箱 X13 套装(箱子,36-蓄热板6块) 
1 set
WEB28645 iP-TEC premium box-mini6.6 (box only)
iP-TEC 精装版保温运输箱 mini6.6(仅箱子)
1 pc
WEB28646 iP-TEC premium box-mini6.6 set ( box,t heat storage-36 3pcs)
iP-TEC精装版保温运输箱 mini6.6 套装 (箱子,36-蓄热板3块)
1 set
WEB28647 iP-TEC premium box-mini6.6 set ( box,t heat storage-24 3pcs)
iP-TEC精装版保温运输箱 mini6.6 套装 (箱子,24-蓄热板3块)
1 set

iP-TEC® 保温运输箱(长距离)

iP-TEC® 保温运输箱(适合长距离运输)

包含多款保温运输箱,用途多样!

每款保温运输箱设有两款蓄热板套装,分别是

→ 箱子+36-蓄热板

箱子+24-蓄热板

可根据运输温度要求选择合适的产品!


—————————————————————————————————————————-

▶iP-TEC® 精装版保温运输箱-迷你6.6(符合运输SOP)

 iP-TEC® premium box-mini6.6(货号:28645)

iP-TEC® 保温运输箱(长距离)

箱盖可收纳温度记录仪和振动记录仪;可上锁。

搭配36-蓄热板使用可保温96小时以上(25℃ 环境),41小时(5℃环境)

◆特点

• 外部尺寸(mm):W335×D245×H270

• 内部尺寸(mm):W240×D165×H165

• 容积:6.6L

• 重量:仅箱子/约1.8kg,3块蓄热板+箱子/约3.8kg

—————————————————————————————————————————-

▶iP-TEC® 精装版保温运输箱-V8.5(轻巧紧凑,易于手持搬运)

 iP-TEC® premium box-V8.5(货号:28482)


iP-TEC® 保温运输箱(长距离)

  iP-TEC® premium box-V8.5是iP-TEC® 系列中顶级的轻巧紧凑型保温运输箱。保温性能极好,可灵活搬运。不止适用于近距离运输,远距离、甚至寒冷地区运输均可使用该款保温箱。



◆特点

外部尺寸(mm):W350×D300×H330 
内部尺寸(mm):W230×D175×H215
容积:8.5L
重量:仅箱子/约3.5kg,5块蓄热板+箱子/约7kg


使用36-蓄热板,可保持 33℃~36℃的培养温度。当外部温度25℃时,保温时长可达 150 小时以上;外部温度5℃时,可达 77 小时。
附有便于搬运的肩带,箱内可收纳6个Flask-25培养瓶。

—————————————————————————————————————————-

iP-TEC® 精装版保温运输箱 V19压倒性的保温性能,运送量大,适合空运

 iP-TEC® premium box-V19(货号:28460)


iP-TEC® 保温运输箱(长距离)

  通过特殊R卷曲技术,使箱子和箱盖的真空隔热材料实现无缝紧密连接,再注入聚氨酯泡沫塑料使隔热效果达到最好。适合远距离和寒冷地区运输。

◆特点

• 外部尺寸(mm):W450×D350×H360 
• 内部尺寸(mm):W330×D235×H250
• 容积:19.4L
• 重量:仅箱子/约4.5kg,6块蓄热板+箱子/约9.5kg


使用36-蓄热板,可保持 33℃~36℃的培养温度。当外部温度25℃时,保温时长可达 150 小时以上;外部温度5℃时,可达 60 小时。

—————————————————————————————————————————-

▶iP-TEC® 标准版保温运输箱-X13(经济实惠,性价比高)

 iP-TEC® standard box-X13(货号:28463)

iP-TEC® 保温运输箱(长距离)

  高性价比。聚苯乙烯泡沫塑料制。可根据要求定制。

◆特点

• 外部尺寸(mm):W366×D356×H394 
• 内部尺寸(mm):W236×D226×H244
• 容积:13L
• 重量:仅箱子/约2.2kg,6块蓄热板+箱子/约6kg

使用36-蓄热板,可保持 33℃~36℃的培养温度。当外部温度25℃时,保温时长可达 100 小时以上;外部温度5℃时,可达 35 小时。


iP-TEC® 保温运输箱(长距离) iP-TEC® 保温运输箱(长距离)
iP-TEC® 活细胞运输系列产品详情 iP-TEC® 系列产品简介列表
iP-TEC® 保温运输箱(长距离) iP-TEC® 保温运输箱(长距离)
iP-TEC® 问题集 蓄热板安装手册

关于精装版保温运输箱-V8.5的产品集
1、
Q:
有往海外运输的实例吗?
A:
有的。有作为航空货物以及带入机内的运输实例。
2、
Q:
是否有质量保证测试?
A:
有的。在【ATA(国际航空运输协会)】的运输标准测试中认定合格。附带ATA信息。
3、
Q:
这种大小的箱子有什么优点?
A:
可用于手持运输。
4、
Q:
有用国内航班运输的实例吗?
A:
有的。
5、
Q:
保温箱大小是否可直接带入飞机?
A:
每家航空公司规定不一样,搭乘时可咨询航空公司。
6、
Q:
保温箱可加入干冰进行运输吗?
A:
可以。
7、
Q:
保温箱是否可以定制?
A:
可以。例如改变真空隔热材料,使用高密度泡沫材料;外箱由铝盖变为硬纸板等。可根据客户的用途和经费提出合适的建议。
8、
Q:
可收纳多少个iP-TEC二次容器PC-0.5或细胞运输盒?
A:
iP-TEC二次容器PC-0.5的话最多可收纳4个,需3个蓄热板。细胞运输盒的话最多2个,需3个蓄热板。
9、
Q:
是否可以交给运输公司进行配送?
A:
可以,旧客户中也有通过一般快递进行运输的。但是,这样的话就变成综合运输了。也有专门从事细胞运输的从业者。本公司与很多快递公司有业务往来,有需要的话可以咨询。
10、
Q:
为了可以GPS追踪,可在运输箱上钻孔吗?
A:
钻孔的话原本的保温、保冷效果可能会减弱,我们是不推荐的。可把通讯装置装在箱子里面或者外面。
11、
Q:
箱子本身怎么灭菌?
A:
箱子交货时是没有灭菌的。使用前请用乙醇擦拭内部进行消毒。
12、
Q:
一年只用一次,是否可出租?
A:
目前没有出租方面的计划。如果以后需求增多的话会考虑提供出租服务。
13、
Q:
外部气温超过40℃有没有影响?
A:
没有影响。如需在高温下使用,请事前咨询厂家蓄热板的放置方法等事宜。
14、
Q:
外部气温超在冰点以下有没有影响?
A:
没有影响。如需在低温下使用,请事前咨询厂家蓄热板的放置方法等事宜。
15、
Q:
为了不让他人打开箱子,是否可以上锁?
A:
另售带锁的安全带。根据所需数量可咨询定做。
16、 Q:
保温箱不使用时应怎样保管?
A:
尽可能放置在阴凉处。阳光直射以及高温湿度大的环境会使保温箱的保温性能劣化。
17、
Q:
保温箱有使用期限吗?
A:
储存条件和运输时的碰撞,长时间下会引起性能下降 。推荐几年为一个周期更新产品。

Hampton蛋白结晶工具及仪器

Hampton蛋白结晶工具及仪器

CryoTong™ – Long
ApplicationsCrystal transfer under cryo temperature
FeaturesArtery clamp style closure30 seconds of cryo temperature180 mm length
Description
The CryoTong is a tool used to manually transfer a crystal mounted on a CrystalCap™ from liquid nitrogen to a magnetic base in a goniometer head positioned in a cryogenic stream, and then back to liquid nitrogen. The one-piece, compact CryoTong is available in two lengths. The long CryoTong is approximately 180 mm in overall length. The artery clamp style maintains the CryoTong in the closed position until the clamp is squeezed, which opens the opposing heads. The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is machined into the lower portion of the head to prevent the CrystalCap from slipping out of the CryoTong when the tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160°C for up to 30 seconds during room temperature crystal transfers. Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray data collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and any other CrystalCap configured for 18 mm.HR5-114 Long CryoTong 18 mm, 180° features the head in alignment with the handle of the CryoTong.

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Vial Clamp™ – Straight
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.
图片
CrystalWand™ Magnetic
ApplicationsCrystalCap Magnetic & CrystalCap Copper Magnetic handling toolDescription
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.HR4-729 CrystalWand Magnetic, StraightHR4-315 CrystalWand Magnetic, 45° (McMiken Tool)

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Vial Clamp™ – Curved
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.HR4-671 Vial Clamp – Curved 45°/135°HR4-672 Vial Clamp – Curved 110°/70°

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CrystalCap™ Holder
pplicationsCrystalCap support inside stainless steel dewars
FeaturesAdjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.HR4-707 CrystalCap Holder – 500 ml
HR4-706  Replacement Height Adjustment Tool

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日本关东化学官网 日本关东化学试剂中国总代理

日本关东化学官网 日本关东化学试剂中国总代理

上海金畔生物科技有限公司是一家销售日本关东化学试剂的中国总代理公司

关东化学株式会社官网 关东化学官方代理商

 

关东化学株式会社官网 关东化学试剂官方授权代理商

关东化学授权代理商

固话总机:021-50837765

订货热线:15221999938

 

qq号:2743691513 1042640511

 

微信号:jinpanbio

 

关东化学株式会社官网 关东化学试剂官方授权代理商

关东化学株式会社官网 中国官方代理

关东化学株式会社是日本著名的综合试剂厂家 它的高质量产品 在国际上享有极高的声誉。不仅在中国国内 多年来在日本国内、亚洲地区、美国、欧洲等地都深受厚爱。为了保证所有试剂产品的质量 关东化学株式会社的全工厂都取得了ISO 9001和ISO/IEC 17025质量管理体系认证 建立了严格的品质管理体系。1998年 全工厂又在日本试剂行业中率先取得了ISO 14001环境管理体系认证。关东化学株式会社不仅是一家拥有先端技术的生产企业 也是一家重视环境安全以及人类社会健康 和谐的可信赖企业。

日本进口小鼠软管一次性灌胃针

发表于2017年12月9日上海金畔生物科技有限公司
seline; width: 584.266px; color: rgb(55, 55, 55);”>上海金畔生物科技有限公司提供5202一次性小鼠灌胃针 M340444

日本进口小鼠软管一次性灌胃针

5202一次性小鼠灌胃针 M340444 详情

日本进口Ptfe软管材质 一次性小鼠灌胃针

货号 规格 针长 针头 外径*内径 针基色 销售单位
5200S 小鼠用 30 1.9 0.90*0.50 250支(5支/小包)箱
5200 小鼠用 38 1.9 0.90*0.50
5202SS 小鼠用 25 2.0 1.18*0.68
5202S 小鼠用 30 2.0 1.18*0.68
5202 小鼠用 38 2.0 1.18*0.68
5204 大鼠用 52 2.8 1.79*1.19
5206 大鼠用 78 2.8 1.79*1.19

 

此灌胃针使用Ptfe软管材质,前端附有硅胶软球。可以有效减少对肺部以及邻近器官的损伤。操作简便。

适用于多种小型实验动物,规格齐全,更可根据您的需求尺寸进行定制。

5根配套小包装,EOG灭菌。

 

动物安全标记笔Animal Marker




使用日本厚生省法定色素,无毒性,经急性经口实验验证对实验动物无伤害

动物安全标记笔Animal Marker :

CatNo. 蓝 绿 红 黄 橙
容量  AM15:15 ml
包装数量 1盒:10支装 (各2支 / 色  可自由配色)

动物安全标记笔:

1,可简便并放心使用的标记笔,使用日本厚生省法定色素。

2,配备了5种高识别度色彩(蓝,绿,红,黄,橙)。

3,对动物皮肤、体毛的渗透力较强,即便被舔舐也不易掉色。

4,一支标记笔用于实验动物老鼠上大致可处理400-600只。

5,绝对保证长期试验过程中不褪色,在一般条件下可保持6-12周的良好辩识度。

seline; width: 584.266px; color: rgb(55, 55, 55);”>固话总机:021-50837765
订货热线:15221999938

qq号:2743691513 1042640511

微信号:jinpanbio
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn

Email:sales@jinpanbio.com

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胃蛋白酶 1:3000 9001-75-6

上海金畔生物科技有限公司提供胃蛋白酶 1:3000 9001-75-6

名称:胃蛋白酶 1:3000
货号:163770
包装:500 g

英文名:Pepsin 1: 3000 from Porcine Stomach
别名:胃液素,胃蛋白酵素,Pepsin A
级别:USP Grade
品牌:金畔生物
CAS:9001-75-6
本品为自猪胃黏膜中提取的胃蛋白酶,为白色或淡黄色的粉末。溶于水,水溶液显酸性。
英文名:Pepsin 1: 3000 from Porcine Stomach
别名:胃液素,胃蛋白酵素,Pepsin A
级别:USP Grade
品牌:金畔生物
CAS:9001-75-6
包装规格
100g,250g,500g,1Kg,更大包装请询价:15221999938
产品优势
进口分装;同时也提供进口品牌产品,例如 Sigma 货号P7125,Amresco 货号0865 ;
应用举例
1、作为助消化药,用作胃蛋白酶片、含糖胃蛋白酶、复方胃蛋白酶颗粒、多酶片等制剂的原料药。
2、胃蛋白酶能催化酪蛋白、球蛋白、组蛋白,动物的角、指甲和羽毛中的角蛋白,以及催化植物蛋白等的水解。除水解天然蛋白质外, 还能水解某些多肽和二肽。在食品、医药、化工、保健品等领域广泛的用作消化剂等。如用于食品加工、鱼粉制造、大豆蛋白的水解,干 酪制造中的凝乳作用(与凝乳酶合用),亦可用于防止啤酒的冷藏混浊等。
3、用作检测某些产品的对照品
相关文档
胃蛋白酶(英文名称:Pepsin)是一种消化性蛋白酶,由胃部中的胃粘膜主细胞(gastric chief cell)所分泌,功能是将食物中的蛋白质分解为小的肽片段。
胃蛋白酶测定可用于鉴别神经性低酸症,当过少或缺乏时,前者胃蛋白酶的含量有时正常而后者盐酸与胃蛋白酶同时缺乏。一般认为胃性低酸症是由于胃粘膜的重症器质性变化所致,特别是对于恶性贫血、无酸症、无胃蛋白酶分泌是诊断上的重要所见。慢性胃炎、慢性胃扩张、慢性十二脂肠炎等胃蛋白酶的分泌常减少。一般胃酸基础分泌高的疾患,如等,胃蛋白酶活性增高。1836年,(Theodor Schwann)在对消化过程进行的研究中,发现了一种能够参与消化作用的,并将其命名为胃蛋白酶。胃蛋白酶也是第一个从动物身上获得的酶。
CAS:9001-75-6;级别/纯度:3000~3500 NFU/mg;英文品名:Pepsin 1:3000;

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方形广口瓶C型


产品编号 产品名称 产品规格 产品等级 产品价格
2133 方形广口瓶C型 1L
2134 方形广口瓶C型 2L

方形广口瓶

方形广口瓶C型

容量

1L

2L

尺寸

82×109×167H mm

85×145×210H mm

口径

44φ mm

44φ mm

Q1: 请问本体、盖子和内盖是用什么材质制成,耐热温度分别是多少?

A1: 本体、盖子和内盖都是由聚乙烯制成,耐热温度是80℃左右。

麦芽淀粉酶标准液 Malt Amylase Standard 货号:E-MAST Megazyme中文站

麦芽淀粉酶标准液

英文名:Malt Amylase Standard

货号:E-MAST

规格:100 mL; α-amylase 950 U/mL;

High purity Malt Amylase Standard for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

A malt extract in which the levels of α-amylase and β-amylase have been standardised. The preparation is designed for use as a standard in the determination of α-amylase and dextrinising power.

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DryKeeper 自动C型干燥箱C-3W(电解法原理)


产品编号 产品名称 产品规格 产品等级 产品价格
WEB0002 DryKeeper 自动C型干燥箱C-3W 白框(电解法原理) 1 set
WEB0001 DryKeeper 自动C型干燥箱C-3B 黑框(电解法原理) 1 set
WEB0003 DryKeeper 自动C型干燥箱C-3BS 遮光(干燥剂除湿法) 1 set
WEB0003NEW DryKeeper 自动C型干燥箱C-3BS 遮光(电解法原理) 1 set
WEB0004 备用隔板(C-3用) 1 pc

DryKeeper 自动C型干燥箱C-3W 白框(电解法原理)

采用高分子电解质膜的水分子电解法,柜内湿度保持在30~40%RH的低湿度范围。

LED灯,低功耗。

W :白框,本体透明


DryKeeper 自动C型干燥箱C-3W(电解法原理)

产品编号

产品名称

规格

WEB0002

DryKeeper 自动C型干燥箱C-3W

1 set

※有效的内部尺寸(mm):255W×338D×452H 

※外部尺寸(mm):扣锁款 317W×392D×617H

※重量:约7㎏


◆特色

● 全自动式,无需更换硅胶。常温下柜内湿度可保持在30~40%RH,适合长时间保管物品。

● 结合模塑部件,功能和强度都得到进一步改善。

● 柜内采用空气循环对流风扇,除湿能力大幅提高。

● 隔板位置可根据保管物品的大小进行调整。(15格,每格28mm)

◆相关产品


WEB0001 DryKeeper 自动C型干燥箱C-3B 黑框(电解法原理)

WEB0003 DryKeeper 自动C型干燥箱C-3BS 遮光(电解法原理)

WEB0003NEW DryKeeper 自动C型干燥箱C-3BS 遮光(干燥剂除湿法)


Millipore密理博PVDF膜 转印膜 Millipore PVDF膜0.45um

Millipore密理博PVDF膜 转印膜 Millipore PVDF膜0.45um

产品详细描述

 

产品名称 品牌货号 规格 备注
Millipore PVDF膜0.45um Millipore IPVH00010 13.25cm*15cm 疏水膜,膜孔径0.45um Immobilon-P Membrane, PVDF, 0.45µm, 26.5cm x3.75m roll
Recommended Replacement for: INCP 000 10(蛋白质应用)
Millipore PVDF膜0.45um Millipore IPVH00010 26.5*3.75m 疏水膜,膜孔径0.45um Immobilon-P Membrane, PVDF, 0.45µm, 26.5cm x3.75m roll
Recommended Replacement for: INCP 000 10(蛋白质应用)
Millipore PVDF膜0.2um Millipore ISEQ00010 13.25cm*15cm 疏水膜,膜孔径0.2um Immobilon-PSQ Membrane, PVDF, 0.2µm, 26.5cm x3.75m roll
Millipore PVDF膜0.2um Millipore ISEQ00010 26.5*3.75m 疏水膜,膜孔径0.2um Immobilon-PSQ Membrane, PVDF, 0.2µm, 26.5cm x3.75m roll

IPVH00010 Qty: 1Immobilon-P 卷膜 PVDF 0.45µm 26.5cmx3.75m 1片/包装Appl: Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析

Immobilon 转印膜

Millipore 提供三种不同的 Immobilon PVDF 膜,是针对不同的蛋白质印迹应用的理想选择。 现在我们还提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。

与***纤维素膜相比,Immobilon 膜具有多种优点。 它们不易破裂或卷 曲,便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外,它们可以重复检测。

Immobilon-P 膜

  • 0.45µm 孔径
  • 建议用于大多数免疫印迹,特别在蛋 白质大于 20kDa 时
  • Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间,将检测时间至少减少了两个小时,而不致降低灵敏 度或特异性

用于高通量实验室的印迹三明治

  • 当您需要处理多个印迹时,印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片Immobilon-P 膜,其中插入了按规格裁切的多张层析级印迹纸。

转印滤纸

  • 按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。

Immobilon-PSQ 膜

  • 0.2µm 孔径和大的内表面面积
  • 较其它膜具有更高的 蛋白质吸附率和测序得率
  • 建议用于小于 20kDa 的印迹蛋白质
  • 防止小分子量的蛋白质的渗漏

Immobilon-FL 膜

  • 首个针对荧光应用进行优化的转印膜
  • 极低的背景提高了所 有荧光检测实验的灵敏度
  • 与所有常用的荧光探针(所有激发和发射波长)兼容
  • 是多态性检测和化学荧光应用的理想选择

性能

使用 Immobilon-P 膜的快速免疫检测实验方案

步骤 标准免疫检测 快速免疫检测
1. 封闭膜 1h
2. 与一抗保温反应 1h 1h
3. 洗膜 3 x 10min 3 x 5min
4. 与二抗保温反应 1h 30min
5. 洗膜 3 x 10min 3 x 5min
6. 加入底物 5min 5min
总时间 4h 5min 2h 5min

Immobilon-PSQ 膜防止渗漏

分子量标准(条带 1、3、5、7)和小牛肝裂解液(条带 2、4、6、8)转印至 Immobilon-P 或 Immobilon-PSQ 膜。 在主膜之后放置了一片 Immobilon-PSQ 来捕获通过(Immobilon- P 之后的条带 5 和6;Immobilon-PSQ 之后的条带 7 和 8)的蛋白质。

Immobilon-FL 膜已针对荧光检测进行优化

Immobilon-FL 膜上的肌动蛋白-微管蛋白多态性检测。 使用兔抗肌动蛋白 1oAB 和 QDot® 655 山羊抗兔2oAB 检测到了兔肌肉肌动蛋白(红色)。 使用鼠抗微管蛋白 1oAB 和 QDot 565 山羊抗鼠 2oAB 检测到了猪脑微管蛋白(绿色)。 两种分析物都检测到灵敏度低于 7ng。 数据由 Quantum Dot Corporation 提供。

技术指标

说明
Immobilon-P 适用于免疫印迹、斑点印迹和其它蛋白质印迹的最佳 PVDF 膜
Immobilon-PSQ 小孔状结构生成 MW <20 kDa 的出众吸附蛋白质
Immobilon-FL 针对荧光免疫检测应用进行了优化
应用
Immobilon-P Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖 蛋白显色和脂多糖分析
Immobilon-PSQ 低分子量 Western blotting、氨基酸分析和
N 端蛋白质测序
Immobilon-FL Western blotting,Dot/Slot blotting
Immobilon Blotting Filter Paper Western blotting, dot/slot blotting
相容的染液/染色剂
Immobilon-P 考马斯亮蓝染色剂
酰胺黑
印度墨水
丽春红
胶体金和 CPTS
CPTS
甲***胺蓝
透射显影和
Sypro® ruby
Immobilon-PSQ 考马斯亮蓝染色剂
酰胺黑
印度墨水
丽春红
胶体金和 CPTS
CPTS
甲***胺蓝
透射显影和
Sypro® ruby
Immobilon-FL 所有荧光染色剂
考马斯亮蓝染色剂
酰胺黑
丽春红
CPTS
Sypro® ruby
Immobilon-P Immobilon-PSQ Immobilon-FL
滤膜材料 Hydrophobic PVDF Hydrophobic PVDF Hydrophobic PVDF
滤膜孔径,µm 0.45 0.45
蛋白质结合量,µg/cm2
胰岛素 160 262 155
BSA 215 340 205
山羊 IgG 294 448 300
订购信息:

比较

目录编号 产品名称 滤膜尺寸,

cm x cm

数量/包装
IPVH07850 Immobilon-P 转印膜 7 x 8.4 50
IPVH08100 Immobilon-P transfer membrane 8 x 10 10
IPVH08130 Immobilon-P transfer membrane 8.5 x 13.5 10
IPVH09120 Immobilon-P Transfer Membrane 9 x 12 10
IPVH10100 Immobilon-P Transfer Membrane 10 x 10 10
IPVH15150 Immobilon-P Transfer Membrane 15 x 15 10
IPVH20200 Immobilon-P Transfer Membrane 20 x 20 10
IPVH304F0 Immobilon-P Transfer Membrane 26 x 26 10
IPVH00010 Immobilon-P Transfer Membrane 26.5 x 375 1
ISEQ07850 Immobilon-PSQ 转印膜 7 x 8.4 50
ISEQ08100 Immobilon-PSQ transfer membrane 8 x 10 10
ISEQ08130 Immobilon-PSQ transfer membrane 8.5 x 13.5 10
ISEQ09120 Immobilon-PSQ transfer membrane 9 x 12 10
ISEQ10100 Immobilon-PSQ Transfer Membrane 10 x 10 10
ISEQ15150 Immobilon-PSQ Transfer Membrane 15 x 15 10
ISEQ20200 Immobilon-PSQ Transfer Membrane 20 x 20 10
ISEQ26260 Immobilon-PSQ Transfer Membrane 26 x 26 10
ISEQ00010 Immobilon-PSQ Transfer Membrane 26.5 x 375 1
IPFL10100 Immobilon-FL 转印膜 10 x 10 10
IPFL20200 Immobilon-FL Transfer Membrane 20 x 20 10
IPFL00010 Immobilon-FL Transfer Membrane 26.5 x 375 1
IPSN07852 Immobilon-P 三明治转印膜 7 x 8.4 20
IPSN08132 Immobilon-P 三明治转印膜 8.5 x 13.5 20
IBFP0785C Immobilon Blotting 滤纸 7 x 8.4 100
IBFP0813C Immobilon Blotting 滤纸

日本关东化学 32033-00 Liquid paraffin 8012-95-1

日本关东化学 32033-00 Liquid paraffin 8012-95-1
日本关东化学代理商–上海金畔生物
代理优势:货期2-3周。
欢迎访问关东化学株式会社Kanto官网或者咨询我们获取更多产品信息。

Product No. 32033-00
Product name Liquid paraffin
Grade Guaranteed reagent for JIS
Package 500mL
Price ¥1,800
Stock 100<=
Product information   JISK9003

Byname Paraffin, Liquid
Physical properties Fp 238℃† d (15℃)0.87†
(△/Specification ▲/Average result †/Litureture)
CAS No. 8012-95-1

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒 D-Mannose/D-Fructose/D-Glucose Assay kit 货号:K-MANGL Megazyme中文站

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒

英文名:D-Mannose/D-Fructose/D-Glucose Assay kit

货号:K-MANGL

规格:55 assays per kit

The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.

UV-method for the determination of D-Mannose, D-Fructose
and D-Glucosein foodstuffs, yeast cell preparations and
other materials

Principle:
(hexokinase)
(1) D-Mannose / D-fructose / D-glucose + ATP →
M-6-P / F-6-P / G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphomannose isomerase) (phosphoglucose isomerase)
(3) M-6-P ↔ F-6-P ↔ G-6-P

Kit size: 55 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 0.7 mg/L
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other
materials (e.g. biological cultures, samples, etc.)
Method recognition: Novel method

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

 Q1. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q5. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q6. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q7. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q8. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q9. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q10. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q11. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q12. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.