合成·反应装置ケミスト广场CPG – 2000系列用セプタム10个入|柴田科技有限公司-环境检测设备、科学仪器的制造销售

产品详细

科学仪器

合成·反应装置ケミスト广场CPG – 2000系列用セプタム10个入

商品代码其他情报(式样)

这个产品比较表中追加
产品照片 合成·反应装置ケミスト广场CPG - 2000系列用セプタム10个入|柴田科技有限公司-环境检测设备、科学仪器的制造销售
商品代码 054310 – 1804 A
型式
价格(不含税) 3,900日元

上海金畔生物科技有限公司

664%2F%3Fc%3D34& ” 664%2F%3Fc%3D34&

一次性医用口腔护理清洁海绵棒 病人老人婴幼儿口腔清洁棒 月子棒

一次性医用口腔护理清洁海绵棒 病人老人婴幼儿口腔清洁棒 月子棒

主营各种特殊性海绵及深加工,提供各种海绵使用方案,以生产经贸一体

 医用一次性海绵:一次性医用口腔清洁海绵棒,一次性口腔护理吸痰管、吸痰管牙刷,一次性医用消毒刷、海绵刷,一次性医用内窥镜海绵,医用敷料海绵

普通海绵:各种颜色、规格、密度、及高难度形状加工(密度6-180KG/M3)。

阻燃海绵:普通阻燃海绵、慢回弹阻燃(防火海绵)、过滤网阻燃绵、高回弹阻燃绵。
慢回弹海绵:各种颜色、硬度、密度30-200KG/M3(枕头、床垫、沙发、
鞋材,可做卷材50M)慢回弹过滤网绵、慢回弹乱孔绵。
高回弹海绵:各种颜色、硬度、密度从30-50KG/M3(沙发、床垫)
聚酯海绵:各种市场上常见品种都可以供应,国产、进口、自行开孔。
木桨海绵:供应进口高质量木桨海绵,多种颜色供选择。
吸水海绵:  医用、化妆用高质量亲水性吸水海绵、PVA、EV等…..
橡 塑 类:CR, EPDM、PORON、橡塑等。

         包装类:聚氨酯海绵,珍珠棉,EVA泡棉,PE泡棉,XPE泡棉,IXPE泡棉等
三聚氰胺海绵: 供国产。进口的三聚氰胺海绵,质量好,价格优,代理销售。
过滤网海绵:供各种密度、颜色、孔径。(10-100PPI)包括聚酯过滤网耐油、汽车、摩托车、割草机、及各种机器设备的空滤,油滤产品。水族、滚筒刷、及特殊性行业用的过滤海绵。
特殊性深加工:上胶,冲型,掏空成型,磨切,缝纫等…

上海金畔生物科技有限公司生产各类海绵棒棒

 

厂家直销 六边形梅花一次性口腔清洁海绵棒 口腔按摩棒 定制上海

 

专业定制,欢迎来电咨询:15221999938

 

上海金畔生物科技有限公司
固话总机:021-50837765
订货热线:15221999938














 

主营各种特殊性海绵及深加工,提供各种海绵使用方案,以生产经贸一体

 

医用一次性海绵:一次性医用口腔清洁海绵棒,一次性口腔护理吸痰管、吸痰管牙刷,一次性医用消毒刷、海绵刷,一次性医用内窥镜海绵,医用敷料海绵

普通海绵:各种颜色、规格、密度、及高难度形状加工(密度6-180KG/M3)。

阻燃海绵:普通阻燃海绵、慢回弹阻燃(防火海绵)、过滤网阻燃绵、高回弹阻燃绵。
慢回弹海绵:各种颜色、硬度、密度30-200KG/M3(枕头、床垫、沙发、
鞋材,可做卷材50M)慢回弹过滤网绵、慢回弹乱孔绵。
高回弹海绵:各种颜色、硬度、密度从30-50KG/M3(沙发、床垫)
聚酯海绵:各种市场上常见品种都可以供应,国产、进口、自行开孔。
木桨海绵:供应进口高质量木桨海绵,多种颜色供选择。
吸水海绵:  医用、化妆用高质量亲水性吸水海绵、PVA、EV等…..
橡 塑 类:CR, EPDM、PORON、橡塑等。

包装类:聚氨酯海绵,珍珠棉,EVA泡棉,PE泡棉,XPE泡棉,IXPE泡棉等
三聚氰胺海绵: 供国产。进口的三聚氰胺海绵,质量好,价格优,代理销售。
过滤网海绵:供各种密度、颜色、孔径。(10-100PPI)包括聚酯过滤网耐油、汽车、摩托车、割草机、及各种机器设备的空滤,油滤产品。水族、滚筒刷、及特殊性行业用的过滤海绵。
特殊性深加工:上胶,冲型,掏空成型,磨切,缝纫等…

 









\




 











专业定制,欢迎来电咨询:15221999938

 

上海金畔生物科技有限公司
固话总机:021-50837765
订货热线:15221999938

 

 

GPC(凝胶渗透色谱)溶剂——1-氯萘


产品编号 产品名称 产品规格 产品等级 产品价格
034-24541 1-Chloronaphthalene 
1-氯萘
1L for GPC

适用于超高温.高温条件下的GPC(凝胶渗透色谱)溶剂

GPC(凝胶渗透色谱)溶剂系列

 


  在溶解或分析聚合物时,溶剂加热后往往会产生脱色。而Wako的GPC溶剂能够保证在溶剂加热后吸光度值不发生改变。同时,Wako的GPC溶剂还能够保证折射率不随着湿度、过氧化物、不挥发成分、杂质含量以及紫外吸收变化而变化。这些特性都使得Wako的GPC溶剂适合用于GPC洗脱液。最后,用0.45μm的滤膜过滤,能获得更高的重现性并延长柱子的使用寿命。

 

加温测试结果

  将Wako的常规1-氯萘与GPC级别1-氯萘同时加热到250℃ 180分钟,常规产品变色,GPC产品不变色。

GPC(凝胶渗透色谱)溶剂——1-氯萘

250℃加热180分钟的外观图

A: GPC用溶剂

B: Wako常规溶剂



1-氯萘规格表

检测项目

规格值

外观

无色透明或黄色液体

吸光度(360nm)

≤0.15

吸光度(400nm)

≤0.05

吸光度(450nm)

≤0.04

吸光度(500nm)

≤0.02

吸光度(550nm)

≤0.02

吸光度(600nm)

≤0.02

GPC实验

适合

水分

≤0.1%

不挥发物

≤0.002%

酸(如HCl)

≤0.001%

过氧化物(如H2O2)

≤0.001%

含量(cGC)

≥95%







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产品编号

产品名

中文名

包装

043-33841

o-Dichlorobenzene

1,2-二氯苯

1L

049-33843

o-Dichlorobenzene

1,2-二氯苯

3L

045-33921

N,N-Dimethylacetamide

N,N-二甲基乙酰胺

1L

041-33923

N,N-Dimethylacetamide

N,N-二甲基乙酰胺

3L

046-33831

N,N-Dimethylformamide

N,N-二甲基甲酰胺

1L

042-33833

N,N-Dimethylformamide

N,N-二甲基甲酰胺

3L

048-33911

Dimethyl Sulfoxide

二甲亚砜

1L

044-33913

Dimethyl Sulfoxide

二甲亚砜

3L

细菌检查试剂|生命科学/临床检验药|关东化学株式会社

医疗·保健卫生·兽医等的临床领域的病原菌检验、食品、医药品等产业领域的产品检查为首,环境检查和研究领域培养基为中心许多的产品。 ISO为首的全世界的标准法和各国标准法依据了处方齐全的英国Oxoid社的培养基,目的菌易懂的颜色显象的佛国家培养基的CHROMagar公司办理的同时,各公司的产品加工容易使用的形状的生培养基、分包培养基独自加工。经营的产品是基础培养基,附录,培养基素材,煤气发生套件,迅速试验用配套元件,鉴别补助试剂,识别试剂,迅速检查用试剂等。

弹出窗口打开

肠管出血性大肠菌

沙门氏菌、痢疾

弯曲杆菌

军团

标准菌株

生命科学/临床检验药的咨询

信息查询

电话: 03 – 6214 – 1091

传真: 03 – 3241 – 1049

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加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售

产品详细

科学仪器

加热块部1 L用300℃规格

加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售

  • 加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售

商品代码其他情报(式样)

这个产品比较表中追加
产品照片 加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售
加热块部1 L用300℃式样|柴田科技有限公司-环境检测设备、科学仪器的制造销售
商品代码 054300 – 3510
型式
价格(不含税) 498,1000日元

上海金畔生物科技有限公司

513%2F%3Fc%3D34& ” 513%2F%3Fc%3D34&

免疫印迹用化学发光试剂ImmunoStar® 系列


产品编号 产品名称 产品规格 产品等级 产品价格
296-69901 ImmunoStar® LD 200cm2 (发光液A:10mL,发光液B:10mL)
292-69903 ImmunoStar® LD 1,000cm2 (发光液A:50mL,发光液B:50mL)
290-69904 ImmunoStar® LD 2,000cm2 (发光液A:100mL,发光液B:100mL)

免疫印迹用化学发光试剂ImmunoStar® 系列

ImmunoStar® Zeta

ImmunoStar® LD

 免疫印迹用化学发光试剂ImmunoStar® 系列

ImmunoStar® 系列是检测过氧化物酶(HRP;POD)标记抗体的免疫印迹用化学发光试剂。

为适应各种实验系统,我们推出了Basic、Zeta和LD三种试剂。

 


◆ImmunoStar® 系列选择指南


产品名称

ImmunoStar® Zeta

ImmunoStar® LD

类型

平衡型

发光强度突出型

发光强度

(灵敏度的标准)

高灵敏度产品

发光强度比Basic高4~20倍

超高灵敏度产品

发光强度比Zeta高20~400倍

发光稳定性

成本

PVDF,硝化纤维素

检测方法

CCD成像仪,X光胶片

检验

可以

特点

1.发光稳定性优良

2.具有优越的定量性能

1.本系列中灵敏度最高



◆ImmunoStar® Zeta


  这是一款非常重视——发光强度、发光稳定性和定量动态范围3者平衡的高灵敏度发光试剂。它在显示强发光信号的同时能持续稳定地发光。而且能在广泛的蛋白数量范围中获得较高线性的标准曲线。

 

发光强度及发光稳定性

  梯度稀释FLAG-BAP,再与其他公司的产品B(高灵敏度产品)的发光强度及发光稳定性作对比。ImmunoStar® Basic显示出的发光信号与其他公司的产品B(高灵敏度产品)相同,但能更持续稳定地发光。

免疫印迹用化学发光试剂ImmunoStar® 系列

定量动态范围


  梯度稀释FLAG-BAP,对比其他公司的产品B的定量动态范围。在与其他公司的产品B同样的蛋白数量范围中ImmunoStar® Basic能作出较高线性的(R2=0.99以上)标准曲线。

免疫印迹用化学发光试剂ImmunoStar® 系列

样本:FLAG-BAP

凝胶:SuperSepTMAce,10~20%,13孔(产品编号:191-15031)

膜:ClearTrans® SP的PVDF膜,疏水性(产品编号:033-22453)

一次抗体:抗DYKDDDDKTag,单克隆抗体(产品编号:014-22383),稀释1,000倍

二次抗体:过氧物酶标记的兔抗鼠IgG抗体(产品编号:018-23643),稀释10,000倍

免疫印迹用化学发光试剂ImmunoStar® 系列

*定量分析作出能获得R2=0.99以上的标准曲线,该范围同时适用于将已测量的目标试剂。

 


◆与其他公司的产品进行比较 发光强度及发光稳定性


  发光强度与其他公司Femtogram级别产品(其他公司的产品D,E)相同水平。而且,在从反应开始90分钟后的检测中,能保持与发光持续性优良的其他公司的产品D相同的发光程度。

免疫印迹用化学发光试剂ImmunoStar® 系列

样本:FLAG-BAP

凝胶:SuperSepTMAce,10-20%,17孔(产品编号:198-15041)

一次抗体:抗DYKDDDDKTag,单克隆抗体(产品编号:014-22383),稀释4,000倍

二次抗体:过氧物酶标记的兔抗鼠IgG抗体(产品编号:018-23643),20,000倍稀释

曝光时间:60秒(LAS4000,standard)

 


◆产品列表


产品编号

产品名称

包装

291-72401

ImmunoStar® Zeta

200cm2 (发光液A:10mL,发光液B:10mL)

297-72403

1,000cm2 (发光液A:50mL,发光液B:50mL)

295-72404

2,000cm2 (发光液A:100mL,发光液B:100mL)

 


ImmunoStar® LD


  这是一款发光强度突出的高灵敏度发光试剂。适用于检测低表达蛋白等微量蛋白。

 

发光强度


免疫印迹用化学发光试剂ImmunoStar® 系列

样本:FLAG-BAP

凝胶:SuperSepTM Ace,10-20%,17孔(产品编号:198-15041)

一次抗体:抗DYKDDDDK Tag,单克隆抗体(产品编号:014-22383),稀释2,000倍

二次抗体:过氧物酶标记的兔抗鼠IgG抗体,稀释20,000倍(产品编号:018-23643)

曝光时间60秒(LAS4000,standard)

ImmunoStar® LD比其他公司的产品B及ImmunoStar® Zeta能更高灵敏度地检测FLAG-BAP。

 

发光稳定性


免疫印迹用化学发光试剂ImmunoStar® 系列

  将1μL稀释8万倍的Anti-Mouse Immunoglobulins/HRP(DAKO, Code No.P0260)加入荧光・发光板中(康宁,Code No.3915),再加入发光试剂各50μL,最后用光度计(TECAN Ultra Evolution)进行测量。

  反应进行120分钟后的发光强度比反应进行5分钟后的反应强度减弱了61%。

 

定量动态范围

  梯度稀释FLAG-BAP,检测出获得较高线性标准曲线的蛋白数量范围。ImmunoStar® LD在0.39~6.25ng的蛋白数量范围中显示出高线性(R2=0.99以上)。

  ImmunoStar® LD的发光强度非常强,信号的减弱速度也相对较快,定量动态范围比ImmunoStar® Zeta小。所以,定量分析目标抗原时最好使用ImmunoStar® Basic或Zeta。

免疫印迹用化学发光试剂ImmunoStar® 系列

样本:FLAG-BAP

凝胶:SuperSepTMAce,10~20%,13孔(产品编号:191-15031)

膜:ClearTrans® SP PVDF膜,疏水性,0.2μm(产品编号:033-22453)

一次抗体:抗DYKDDDDKTag,单克隆抗体(产品编号:014-22383),稀释1,000倍

二次抗体:过氧物酶标记的兔抗鼠IgG抗体(产品编号:018-23643),稀释40,000倍

免疫印迹用化学发光试剂ImmunoStar® 系列

*定量分析作出能获得表示决定系数R2=0.99以上的线性的标准曲线,该范围同时适用于将已测量的目标试剂。。

在抗原量0.39~6.25ng(Lane No.1~5)的范围内,R2=0.99以上(1.2order)。



◆产品列表


产品编号

产品名称

包装

296-69901

ImmunoStar® LD

200cm2   (发光液A:10mL,发光液B:10mL)

292-69903

1,000cm2   (发光液A:50mL,发光液B:50mL)

290-69904

2,000cm2   (发光液A:100mL,发光液B:100mL)

 

美国pall 尼龙滤膜1.2um孔径NNG025100 25MM NNG047100 47MM

美国pall 尼龙滤膜1.2um孔径NNG025100 25MM

美国pall 尼龙滤膜1.2um孔径NNG047100 47MM
美国PALL尼龙膜1.2um孔径NNG047100 直径47mm
pall ULTIPORN Membrane Filters
油品清洁度检测专用滤膜NNG025100,25mm*1.2um,100张/盒,尼龙(Nylon)材质
油品清洁度检测专用滤膜NNG047100,47mm*1.2um,100张/盒,尼龙(Nylon)材质

L-苹果酸[AF]检测试剂盒 L-Malic Acid Assay Kit (Analyser Format) 货号:K-LMALAF Megazyme中文站

L-苹果酸[AF]检测试剂盒

英文名:L-Malic Acid Assay Kit (Analyser Format)

货号:K-LMALAF

规格:245.5 mL of prepared reagent (e.g. 1 assay

The L-Malic Acid (Analyser Format) test kit is an analyser format for the specific measurement and analysis of L-malic acid (L-malate) in beverages and food products. On calibration, the prepared reagent is linear to > 80 micrograms of L-malic acid per mL of assay solution.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Analyser format UV-method for the determination of L-Malic Acid
in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size: 245.5 mL of prepared reagent (R1 + R2)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 20 mg/L (recommended assay)
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

  • PVP incorporated to prevent tannin inhibition
  • Very stable reagent when prepared for auto-analyser applications
  • Linear calibration (R2 ~ 0.9994) up to 80 μg/mL of L-malic acid in final reaction solution
  • Validated by the University of Wine, Suze la Rousse, France
  • Very competitive price (cost per mL of reagent)
  • Both enzymes supplied as stable suspensions
  • Very rapid reaction (~ 3 min)

Q1. What is the difference between K-LMAL-58A / 116A, K-LMALAF, K-LMALMQ and K-LMALQR?

Megazyme produces 4 L-malic acid test kits:
K-LMAL-58A / 116A: UV method, automated format for use with auto-analysers.
K-LMALAF: UV method, manual format for use with spectrophotometers.
K-LMALMQ: Colourimetric method, manual format for use with hand held colorimeter.
K-LMALQR: UV method, liquid ready reagents automated format for use with auto-analysers.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. Do samples require any specific sample preparation prior to testing with the kits?

The sample preparation is sample dependent, some samples may be tested directly in the assay or after appropriate dilution, however, some samples may require further sample preparation prior to testing.  The following are examples of sample preparation methods:
(a) Liquid samples: clear, slightly coloured and approximately neutral, liquid samples can be used directly in the assay.
(b) Acidic samples: if > 0.1 mL of an acidic sample is to be used undiluted (such as wine or fruit juice), the pH of the solution should be increased to approx. 9.0 using 2 M NaOH, and the solution incubated at room temperature for 30 min.
(c) Carbon dioxide: samples containing significant quantities of carbon dioxide, such as beer, should be degassed by increasing the pH to approx. 9.0 with 2 M NaOH and gentle stirring, or by stirring with a glass rod.
(d) Coloured samples: an additional sample blank, i.e. sample with no L-MDH, may be necessary in the case of coloured samples.
(e) Strongly coloured samples: if used undiluted, strongly coloured samples should be treated by the addition of 0.2 g of PVPP/10 mL of sample.  Shake the tube vigorously for 5 min and then filter through Whatman No. 1 filter paper.
(f) Solid samples: homogenise or crush solid samples in distilled water and filter if necessary.
(g) Samples containing fat: extract such samples with hot water at a temperature above the melting point of the fat, e.g. in a 100 mL volumetric flask.  Adjust to room temperature and fill the volumetric flask to the mark with distilled water.  Store on ice or in a refrigerator for 15-30 min and then filter.  Discard the first few mL of filtrate, and use the clear supernatant (which may be slightly opalescent) for assay. Alternatively, clarify with Carrez reagents.
(h) Samples containing protein: deproteinise samples containing protein by adding an equal volume of ice-cold 1 M perchloric acid with mixing.  Centrifuge at 1,500 g for 10 min and neutralise the supernatant with 1 M KOH. Alternatively use Carrez reagents.

Q6. Which L-Malic Acid Kit is recommended for a 96-well microplate format?

Auto-analysers use ~ 0.315 mL reaction volumes and pathlengths between 4-8 mm which is similar to a standard 96-well microplate where a 0.315 mL reaction volume would give a pathlength of ~ 6-7 mm.  Therefore, K-LMALAF can be used directly in a 96-well microplate format with minimal assay optimisation.
If preferred, K-LMAL-58A / 116A may also be easily converted for use in a 96-well microplate format.  Basically, the assay volumes for the cuvette format must be reduced approximately 10-fold for use in a 96-well microplate.  However, some assay optimisation may be required (e.g. increased enzyme concentration etc.) and unlike the cuvette which has a set pathlength of 1 cm, the pathlength in the microplate is dependent upon the volume of liquid in the well.
Therefore to enable the calculation of the amount of analyte in the samples from tests performed in the microplate format one of the following must be done:

  1. The easiest method is to use a microplate reader that has a pathlength conversion capability (i.e. the microplate reader can detect the pathlength of each well and convert the individual readings to a 1 cm pathlength).  This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm pathlength) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values.

L-Malic Acid Kit Recommendation For Microplate Format:
Either K-LMAL-58A / 116A or K-LMALAF is recommended for use in a 96-well microplate format and the main advantages / disadvantages are described below:
K-LMAL-58A / 116A:
The assay volumes of this kit should be reduced by 10-fold for use in a 96-well microplate format (some assay optimisation may be required, e.g. increased enzyme concentration etc.).
The calculation of results is achieved as outlined above in either of points 1, 2 or 3.
The main advantage here is that if this kit is used with a microplate reader that has a pathlength conversion capability or if results are converted as outlined above in point 3 then this enables easy calculation of results using the K-LMAL-58A / 116A MegaCalc application (available on the Megazyme website where the product is located).
K-LMALAF:
This kit is designed for use in an auto-analyser and therefore can be used without any modification to assay volumes directly in a 96-well microplate format.  This kit has less reagent additions than K-LMAL-58A / 116A. 
K-LMALAF does not have a MegaCalc application available to enable easy results calculation which therefore must be achieved as outlined above in either of points 2 or 3.

Q7. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the 
K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch 
this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q8. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q9. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

wako HiPolypeptone (Polypeptone)多聚蛋白胨

wako HiPolypeptone (Polypeptone)多聚蛋白胨

同义名:Hipolypepton;Polypepton;Casein Peptone;多聚蛋白胨;高聚蛋白胨;聚蛋白胨;酪蛋白胨;

HiPolypeptone (Polypeptone) 多聚蛋白胨 日本制药株式会社 392-02115

生厂商:Nihon Pharmaceutical Co. Ltd. 日本制药株式会社(隶属于Takeda Pharmaceutical Group 武田制药株式会社)

基本描述:

HiPolypeptone (Polypeptone) 多聚蛋白胨用作微生物培养用,可配置各种微生物培养基,用于细菌的培养、分离、增殖、鉴定。以及无菌实验培养基,厌氧菌培养基等细菌生化特性实验用培养基的配置。

产品特点:

制造工艺:对主原料(酪蛋白)经酶分解,经过滤、浓缩、提纯,干燥而得的粉末;

原料来源:新西兰产地(未发生疯牛病)的酪蛋白;

工艺严控:本品由日本四大药业之一的Nihon Pharmaceutical生产,严格按照日本药局方的药品生产规范制备;

质控标准:

外观 Grayish yellow powder
溶解性(5% 水溶液)) Clear Solution
重金属(如Pb)) ≤1ppm
干燥失重 ≤6.0%
炽灼残渣 ≤10%
总氮(T-N) ≥11%
氨基酸氮( A-N) 5~7%

 

订购信息:原装正品,现货供应。欢迎来电咨询上海金畔生物科技有限公司

品牌 货号 名称 规格 价格(元)
日本制药株式会社 392-02115 HiPolypeptone 多聚蛋白胨 500g 咨询

日本进口wako多聚蛋白胨Hipolypepton

品牌: 日本制药
货号: 392-02115,392-02117
保存条件: 室温
供应商: Wako
规格: 500g/20kg
规格: 500g 产品价格: 询价
规格: 20kg 产品价格: 询价

上海金畔生物供应日本制药的多聚蛋白胨系列产品,包括以新西兰产地的酪蛋白为原料的培养基“酪蛋白胨”,和以大豆为原料的“酪蛋白胨S”“酪蛋白胨N”“酪蛋白胨NS”。
品名:多聚蛋白胨
英文名称:Hipolypepton

品牌: 日本和光纯药
货号: 392-02115,392-02117
保存条件: 室温
供应商: Wako
规格: 500g/20kg

 

规格: 500g 产品价格: 询价
规格: 20kg 产品价格: 询价

本系列是将主原料进行酶分解后,提纯、干燥而成的粉末。“Hipolypepton”(酪蛋白胨)的原料酪素钠仅使用未发生过疯牛病的新西兰产区的牛。可配制各种微生物培养基,用于细菌的培养、分离、增殖、鉴定,以及无菌试验培养基、厌氧菌培养基等细菌生化特性试验用培养基的配置。

胰蛋白胨还广泛应用于高品质的抗生素、维生素、医药工业,氨基酸、有机酸、酶制剂、黄原胶等发酵工业,生化制品及微生物学科研等领域中。临床用于抗炎消肿,工业上用于皮革制造,生丝处理,食品加工。

酪蛋白胨

产品名称 酪蛋白胨
规格 500g 20kg
产品编号 392-02115 398-02117
价格 590 咨询
主原料 酪素钠
动物酶
制造方法 主原料一酶分解一过滤一浓缩一干燥一分装
X产品性质 性状 外观 淡黄白色一淡黄褐色的粉末
气味 气味特别,但无腐臭
溶液澄清度及色泽 淡黄色一黄色澄洁
干燥减量 6%以下
炽灼残渣 10%以下
总氮(T-N) 11%以上
氨基酸氮( A-N) 5-7%

大豆蛋白胨

产品名称 酪蛋白胨S 酪蛋白胨N 酪蛋白胨NS
规格 500g 20kg 300g 10kg 300g 10kg
产品编号 394-02175 390-02177 397-02121 395-021 27 393-02101 301·02107
价格 700 咨询 540 咨询 700 备询
主原料 脱脂大豆 大豆萃取物 脱脂大豆
动植物酶 微生物酶 微生物酶
制造方法 主原料一酶分解—过滤—浓缩—干燥—分装
产品性质 性状 外观 淡黄白色。淡黄褐色的粉末
气味 气味特别,但无腐臭
溶液澄清度丑色泽 淡黄色·黄色澄清 淡黄色·褐色澄清 淡黄色澄清
干燥减量 6%以下 6%以下 9%以下
炽灼残渣 23%以下 15%蹦下 23%以下
总氨( T-N) 7%以上 12%以上 7%以上
氨基酸氮( A-N) 3-5% 5-7% 3-5%

 

产品编号 产品名称 规格
392-02115 酪蛋白胨 500g
398-02117 20kg
394-02175 酷蛋白胨S 500g
390-02177 20kg
397-02121 酪蛋白胨N 300g
395-02127 10kg
393-02101 酪蛋白胨NS 300g
391-0210/ 10kg

 

产品编号 产品名称 规格
394-02131 酵母浸出粉SH 250g
392-02137 10kg
398-02151 酵母浸出粉D-3H 250g
396-02157 10kg
393-02167 酵母浸出粉FH 10kg
393-02145 酪蛋白氨基酸“DAIGO’ 500g
399-02147 10kg

 

淀粉总量检测试剂盒 Total Starch (AA/AMG) Assay Kit 货号:K-TSTA-100A Megazyme中文站

淀粉总量检测试剂盒

英文名:Total Starch (AA/AMG) Assay Kit

货号:K-TSTA-100A

规格:100 assays per kit

分析物意义:主要的食品组分

Megazyme检测试剂盒优点:选择用GOPD试剂或己糖激酶或6-磷酸葡萄糖脱氢酶测定D-葡萄糖的快速检测试剂盒

The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).

Colourimetric method for the determination of Total Starch in
cereal products, feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C ± DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard Method
No. 168), and RACI (Standard Method)

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 12 months after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Why does the quadruplicate glucose control in your Total Starch Assay Kit have to be incubated?

We feel more comfortable with quadruplicate glucose controls.  If the control is incorrect, or questionable, then all the results are in doubt.

Q3. Why do duplicate samples have to be measured?

Duplicate samples do not have to be measured.  We just suggest this for laboratories starting up. 

Q4. Does the Regular Maize Starch need to be analysed with pre-treating by DMSO? How do you store this Enclosed Control?

The Regular Maize Starch does not require DMSO pre-treatment.  The value should be about 84% with a moisture content of about 12%, the final dry weight value is about 96-97%.  Store the sample at room temperature, dry.

Q5. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q6. Does your kit with DMSO solubilise starch that has been vitrified due to malting/kilning?

Yes.  We believe that the DMSO step will solubilise vitrified starch in malt.  Make sure that the malt is milled to pass a 0.5 mm screen.  You could vary the time of cooking with DMSO to check solubilisation (i.e. 5 minutes, 10 minutes, or even up to 1 hour).

Q7. Is it possible to differentiate between gelatinised and ungelatinised starch in finished products, such as dog food, using the Total Starch Kit?

We think that there is a better chance of success using the Megazyme Starch Damage Kit.

Q8. Is it possible to use the Total Starch Kit to measure starch levels in plasterboard and related products?

There should be no problem in measuring the starch in plasterboard.  I suggest that you grind about 100 g in a kitchen blender and then fine mill to pass 0.5 mm screen. Run a standard assay, but adjust volume to 10 mL after alpha-amylase treatment. Keep a close check on the pH.  Plasterboard may push the pH value up (pH up to about 8 should be fine).  You may be advised to run a DMSO format concurrently just to be sure.  When you treat with amyloglucosidase, I would advise that you take 0.2 and 0.4 mL aliquots of digest (to get the colour up), also, be careful about checking the pH.

Q9. Can the Total Starch Kit be used for samples containing 20% fat or higher?

A 20% fat content could cause a problem for the method.  We suggest that the sample be defatted before analysis for starch.

Q10. Do you have any kit or procedures for the determination of extractable starch in corn? This is of particular concern in the corn wet milling. I presume that extractable starch in corn is not the same as total starch?

Our Total Starch Assay Kit could measure starch left in a residue, or starch extracted. No method could measure potential extractable starch, as this will depend on numerous factors, including processing equipment, conditions etc.

Q11. Can I use another buffer instead of the MOPS with your Total Starch Assay Kit? If so, which would be suitable and easily prepared from commonly available laboratory reagents?

You can use phosphate buffer at the same concentration.

Q12. I wish to measure Total Starch in several products. These products contain 10-20% starch + maltodextrins at similar levels. Is it possible to remove the maltodextrins from the sample? Will ethanol work?

Most of the maltodextrins can be removed with 50% ethanol washing.  If the starch is not gelatinised, it can be washed with cold water.  This will remove all of the soluble maltodextrins, but the starch will spin down.  If the starch has been gelatinised, then the best material which can be used for washing is 50% ethanol.

Q13. Could your Total Starch Assay Kit (K-TSTA) be used with success to measure total starch in plant tissues (samples of roots and shoots of maple trees)?

Yes, the Total Starch Kit can be used to measure starch in roots and shoots etc. 

Q14. Is the accuracy of the Total Starch test affected by the presence of other inorganic chemicals and ground calcium carbonate in pulp?

We think that calcium carbonate etc. will not cause any problems.  However, this of course depends on the amount present and if it changes the pH of the incubation mixture.

Q15. Does the Megazyme Total Starch method work well on all the new chemically modified starches that are now appearing, e.g. highly crosslinked, dextrinised and highly propylene oxide substituted?

The method will work for some chemically modified starches (e.g. crosslinked) however, if the degree of chemical modification is high, there will be an underestimation as the modification will interfere with complete hydrolysis to glucose and subsequent measurement.

Q16. Is it necessary to pre-wash ground cereal samples prior to analysis for Total Starch?

You only need to wash samples which you feel may contain glucose and/or maltodextrins, e.g. breakfast cereals.  There is little glucose in ground cereals, so it is not necessary to pre-wash these materials.

Q17. What is the stability of the enzymes from the Total Starch Kit?

The enzymes from this kit are stable at room temperature for at least 6 months.  At 4˚C, they are stable for several years.

Q18. Can the Total Starch Kit determine the degree of gelatinisation? Sample : Corn Flour.

The Starch Damage Kit may be best for this.  If the starch is gelatinised and dried before analysis the correct results for gelatinisation will not be obtained.

Q19. Are there any limits to the sensitivity of the Total Starch Kit?

The Total Starch Kit can accurately measure starch levels as low as 1% w/w.

Q20. What is the sensitivity and how much is the absorbance of glucose standard (100 micrograms)?

The absorbance for 100 micrograms of glucose (in 3 mL of GOPOD Reagent) is about 0.97.

Q21. Is it possible to raise sensitivity by modifying dilution of GOPOD reagent?

Yes, you can reduce the volume of GOPOD to 1 mL and use micro cuvettes.  This will increase sensitivity by ~ 3-fold.

Q22. Does DMSO solubilise resistant starch, i.e. crystallised amylose and amylopectin?

DMSO does solubilise resistant starch (crystallise amylose and amylopectin).  The only starch material we have had problems in dissolving in DMSO is potato amylose.

Q23. When analysing samples containing sugars, an 80% v/v solution of ethanol is used to solubilise and remove the sugars. About how large are the smallest dextrins that are left in the starch (not solubilised) in this treatment?

We believe that for starch fragments, oligosaccharides of a DP up to 10 would be soluble in 80% alcohol.  The degree of solubility of other oligosaccharides would depend on the sugar type and linkage type.

Q24. What is the sensitivity of the Total Starch Method for measurement in liquids containing low levels of starch?

The Total Starch Kit can be used for liquids containing as little as 200 micrograms per mL with some adjustments of conditions, as below:
Mix 0.5 mL of sample with 0.5 mL of 100 mM sodium acetate buffer (pH 4.5). Incubate at 40˚C and add 0.1 mL of Amyloglucosidase and incubate for 30 minutes.  Add GOPOD reagent as usual.  You will need to run an AMG blank as this enzyme preparation contains a very small amount of glucose.

Q25. AMYLOGLUCOSIDASE. The activity is stated as being 3260 U/mL (Soluble Starch). How was this determined?

The AMG activity was determined with soluble starch as substrate (10 mg/mL) in 0.1 M sodium acetate buffer at pH 4.5 and 40˚C.  One Unit is the amount of enzyme required to hydrolyse one micromole of maltose per minute (i.e. to release 2 micromoles of glucose).  Glucose release is measured with Glucose Determination Reagent.

Q26. I wish to know if it is possible to perform the assay under acidic conditions? I also need to alter the pH of the MOPS/amylase mixture to pH 3 or 4. Is it known if the amylase supplied with the Total Starch Assay Kit has activity at such a low pH?

We can assure you that the Total Starch Kit will not work if incubations with the thermostable alpha-amylase are performed at pH 3 or 4.  This enzyme is inactivated at pH values below 5.0.  You may wish to look at a method using just amyloglucosidase which is quite active down to pH 4.0.  Check the old AOAC procedure for starch.

Q27. We are currently using your kits for Total Starch, Starch Damage and Sucrose/Glucose/Lactose Assay procedures. How accurate are the volumes contained? Can they be diluted entirely, to avoid wasting the contents while transferring?

When we dispense the enzymes we usually include an extra 5% in each vial, so yes, you can dilute the whole vial.  When you do this, please divide into aliquots and store them frozen.

Q28. Which analytical procedure would you recommend for determination of total starch residues in brewing wort and final beer. Megazyme’s procedure – AA/AMG’97, only refers to solid (and not solubilised) starch.

You can use the standard procedure. We would recommend that you treat 2 mL of beer or wort with 8 mL of ethanol, stir and centrifuge (3,000 rpm).  Wash the pellet with 10 mL of 80% ethanol.  Then dissolve/suspend the pellet in 2 mL of sodium acetate buffer (pH 4.5, 0.1 M) and cook at 100˚C for 10 min.  Then add 0.1 mL AMG from the Total Starch Kit and proceed according to the method.  You will have to determine the degree of dilution for yourself.  Treat 0.1 mL with GOPOD etc.