乙酸[AK法]检测试剂盒 Acetic Acid (AK; analyser format) 货号:K-ACETAK Megazyme中文站

乙酸[AK法]检测试剂盒

英文名:Acetic Acid (AK; analyser format)

货号:K-ACETAK

规格:170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL)

分析物意义: 常见食品的组分

Megazyme检测试剂盒优点: K-ACETRM 是运用AK和磷酸乙酰转移酶的新型、快速的手工检测试剂盒。试剂稳定

K-ACETAK (自动) 是一种以乙酸激酶(AK)为基础的,新型、稳定、快速的检测试剂盒,具有良好的线性。

 

Analyser format for the specific assay of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution. Content:170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL)

Analyser format UV-method for the determination of Acetic Acid
in foodstuffs, beverages and other materials

Principle:
(acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(D-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → D-lactic acid + NAD+

Kit size: 550 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 10 min
Detection limit: 10 mg/L (recommended assay format)
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables,
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products
(and baking agents), ketchup, soy sauce, mayonnaise, dressings,
paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions),
feed and other materials (e.g. biological cultures, samples, etc.)
Method recognition: Improved method

Advantages

  • Very stable reagent when prepared for auto-analyser applications (> 7 days at 4°C)
  • PVP incorporated to prevent tannin inhibition
  • Linear calibration (R2 ~ 0.9995) up to 30 μg/mL of acetic acid in final reaction solution
  • Validated by the University of Wine, Suze la Rousse, France
  • Very rapid reaction
  • Very competitive price (cost per mL of reagent)
  • All reagents stable for > 2 years

  • Extended cofactors stability

 

1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and   therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

3. Does the decolourising preparation remove some VA during the process?

No, however the sample preparation process can be tested by adding a known amount of acetic acid standard and assessing the recovery of this. 

4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

5. What are the major the differences between the various acetic acid test kits?

Megazyme produces 4 acetic acid test kits:
K-ACET: uses the traditional ACS reaction.  Manual format for use with spectrophotometers.
K-ACETAF: uses the traditional ACS reaction.  Automated format for use with auto-analysers.
K-ACETAK: uses the more recently developed and more rapid acetate kinase reaction.  Automated format for use with auto-analysers.
K-ACETRM: uses the more recently developed and more rapid acetate kinase reaction.  Manual format for use with spectrophotometers. 

6. Can acetic acid be measured in culture/fermentation media?

Acetic acid in liquid cell culture media/supernatants or fermentation samples can be determined without any sample treatment (except clarification by centrifugation or filtration) and appropriate dilution in distilled water. 

7. Which acetic acid kit is recommended for a 96-well microplate format?

Auto-analysers use ~ 0.315 mL reaction volumes and pathlengths between 4-8 mm which is similar to a standard 96-well microplate where a 0.315 mL reaction volume would give a pathlength of ~ 6-7 mm.  Therefore K-ACETAK or K-ACETAF can be used directly in a 96-well microplate format with minimal assay optimisation.
If preferred, K-ACET or K-ACETRM may also be easily converted for use in a 96-well microplate format.  Basically, the assay volumes for the cuvette format must be reduced approximately 10-fold for use in a 96-well microplate.  However, some assay optimisation may be required (e.g. increased enzyme concentration etc.) and unlike the cuvette which has a set pathlength of 1 cm, the pathlength in the microplate is dependent upon the volume of liquid in the well.  Therefore to enable the calculation of the amount of analyte in the samples from tests performed in the microplate format one of the following must be done:

  1. The easiest method is to use a microplate reader that has a pathlength conversion capability (i.e. the microplate reader can detect the pathlength of each well and convert the individual readings to a 1 cm pathlength).  This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm pathlength) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values.


Acetic Acid Kit Recommendation For Microplate Format:
Either K-ACETRM or K-ACETAK is recommended for use in a 96-well microplate format and the main advantages / disadvantages are described below:
K-ACETRM:
The assay volumes of this kit should be reduced by 10-fold for use in a 96-well microplate format (some assay optimisation may be required, e.g. increased enzyme concentration etc.).
The calculation of results is achieved as outlined above in either of points 1, 2 or 3. 

7. Is the acetic acid kit specific for acetate?

Propionate may react more slowly than acetate.

8. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

9. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

10. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

11. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q12. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q13. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q14. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefor

Alpha淀粉酶检测底物 Amylazyme – 1000 Tablets 货号:T-AMZ-1000T Megazyme中文站

Alpha淀粉酶检测底物

英文名:Amylazyme – 1000 Tablets

货号:T-AMZ-1000T

规格:1000 Tablets

High purity dyed and crosslinked Amylazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

AACC Method 22-05.01 and RACI Standard Method. For the assay of cereal and microbial α-amylase. Containing AZCL-Amylose. Recommended substrate for the assay of α-amylase in weather-damaged cereal grains, honey samples and food products containing low levels of this activity.

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可吸收碳水化合物检测试剂盒 Available Carbohydrates/Dietary Fiber Assay Kit 货号:K-ACHDF Megazyme中文站

可吸收碳水化合物检测试剂盒

英文名: Available Carbohydrates/Dietary Fiber Assay Kit

货号:K-ACHDF

规格:100 assays of each component

分析物意义:  快速消化和吸收的糖及膳食纤维

Megazyme检测试剂盒优点:检测程序新颖、试剂稳定 

An integrated procedure for the measurement of available carbohydrates and dietary fibre in cereal products, fruit and vegetables and food products. Content:100 assays of each component

An integrated procedure for the measurement of Available
Carbohydrates and Dietary Fiber in cereal products, fruit and
vegetables and food products

Principle (Dietary Fiber):
(α-amylase + amyloglucosidase)
(1) Starch + H2O → glucose

(protease)
(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following
alcohol precipitation


Principle (Available Carbohydrates):
(sucrase / maltase + β-galactosidase)
(4) Sucrose, maltose and lactose → D-glucose + D-fructose
+ D-galactose


(PGI, hexokinase + glucose-6-phosphate dehydrogenase)
(5) D-Glucose + D-fructose + ATP + NADP+ → gluconate-6-phosphate
+ NADPH + ADP + H+

Kit size: 100 assays of each
Application examples:
Food ingredients, food products and other materials
Method recognition:
Dietary Fibre - AOAC (Methods 985.29, 991.42, 991.43 and 993.19)
and AACC (Methods 32-05.01, 32-07.01 and 32-21.01)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • High purity / standardised enzymes employed
  • Only kit available
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Simple format

 Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

wako日立氨基酸分析仪配套试剂

日立氨基酸分析仪配套试剂解决方案

日立氨基酸分析仪在国内应用较为普遍,有不少人在选择配套试剂时存在各种问题,难以取舍。上海金畔生物提供日立氨基酸分析仪配套试剂-缓冲液、显色液的解决方案。

详细信息

20140827FR4XP4JV82

日立氨基酸分析仪配套试剂

       日立氨基酸分析仪在国内应用较为普遍,国内使用的型号主要为L-8500型,L-8500A型,L-8800型,L-8900型等多种型号,采用柱后衍生、用茚三酮显色液作为衍生试剂的离子交换色谱法,配套缓冲液作为洗脱试剂的工作方式。有不少人在选择配套试剂时存在各种问题,难以取舍。希望以下有关如何选择日立氨基酸分析仪配套试剂的一些小窍门能对您有所帮助。
配套试剂的构成

       日立(Hitachi)本身并不生产试剂,其仪器的配套试剂都是由日本的两家大型化学试剂生产商(和光WAKO和三菱MCI)为其生产。不论是什么型号的配套试剂,基本都分3部分:
2014082702H28DX0BP
 上海生物提供日立氨基酸分析仪配套试剂缓冲液、显色液。
货号
品名
包装
608-07461 MCI Buffer L-8500-PH Kit
包含:PH-11L*2, PH-21L*1, PH-31L*1, PH-41L*2, PH-RG1L*1
1KIT
605-07471 MCI Buffer L-8500-PF Kit
包含:PF-11L*2, PF-21L*1, PF-31L*1, PF-41L*2, PF-RG1L*1
1KIT
632-07401 MCI Buffer L-8900 PFS Kit
包含:PFS-1 1L*2, PFS-2 1L*2, PFS-3 1L*1, PFS-4 1L*1, PFS-RG 1L*1
1KIT
299-70501 Ninhydrin Coloring Solution Kit For HITACHI
日立氨基酸分析仪配套茚三酮显色液(L-8500,L-8900均适用)
1L*2
013-08391 Amino Acids Mixture Standard Solution, Type H
氨基酸混合标准溶液, H型
5ml
015-14461 Amino Acids Mixture Standard Solution, Type AN-2
氨基酸混合标准溶液, AN-2型
5ml
016-08641 Amino Acids Mixture Standard Solution, Type B
氨基酸混合标准溶液, B型
5ml
wako日历氨基酸分析仪配套试剂

wako日历氨基酸分析仪配套试剂

 

缓冲液

  缓冲液是氨基酸分析的重要试剂之一,虽然主要成分是柠檬酸、柠檬酸钠、氯化钠等常见有机试剂,但对于试剂的纯度,配置有一定要求。试剂不当,轻则影响试验结果,重者造成仪器异常,甚至损伤。原装配套的缓冲液和显色液在试剂纯度,稳定性上都有保证,且省去了配置试剂的时间,可以大大提供测定准确的和工作效率。
  日立前期推出的包括835型,L-8500型,L-8500A型,L-8800型氨基酸分析仪配套的缓冲液都分PH和PF系列,适用于不同的样本和检测目的。L-8900型可以使用L-8500型的配套试剂,但是如果希望具有高吞吐能力,则需要使用L-8900型专用的PFS系列。
  • PH系列: 适用蛋白质水解产物分析
  • PF系列: 适用生物液体分析
  • PFS系列: 适用生物液体分析,相比PF具有高吞吐能力
 显色液

  日立氨基酸分析仪配套的茚三酮显色液(Ninhydrin Coloring Solution Kit),适合L-8500,L-8800和L-8900等多种型号日立氨基酸分析仪。
L-8500 L-8900
PH系列缓冲液
PF系列缓冲液
PFS系列缓冲液
茚三酮显色液
生物液体 蛋白水解液 生物液体 蛋白水解液 高吞吐*
茚三酮显色剂+缓冲液套装
如何选择合适的专用配套试剂

(1)符合下列条件1,适合使用以下配套试剂:
  • 仪器是L-8500型,L-8500A型,L-8800型
  • 检测样本为蛋白质水解产物
608-07461 MCI Buffer L-8500-PH Kit
(包含:PH-1 1L*2, PH-2 1L*1, PH-3 1L*1, PH-4 1L*2, PH-RG 1L*1)
1KIT
634-12471 MCI Buffer L-8500-PH-1 1L×2
604-07463 1L×10
608-07466 MCI Buffer L-8500-PH-2 1L×10
631-12481 1L×2
638-12491 MCI Buffer L-8500-PH-3 1L×2
606-07462 1L×10
631-12501 MCI Buffer L-8500-PH-4 1L×2
602-07464 1L×10
638-12511 MCI Buffer L-8500-PH-RG 1L×2
600-07465 1L×10
299-70501 NINHYDRIN COLORING SOLUTION KIT FOR HITACHI FOR 2L
(2)符合下列条件2,适合使用以下配套试剂:
  • 仪器是L-8500型,L-8500A型,L-8800型
  • 检测样本为生物液体
605-07471 MCI Buffer L-8500-PF Kit
包含:PF-1 1L*2, PF-2 1L*1, PF-3 1L*1, PF-4 1L*2, PF-RG 1L*1
1 kit
601-07473 MCI Buffer L-8500-PF-1 1L×10
639-12421 1L×2
607-07475 MCI Buffer L-8500-PF-2 1L×10
636-12431 1L×2
633-12441 MCI Buffer L-8500-PF-3 1L×2
605-07476 1L×10
630-12451 MCI Buffer L-8500-PF-4 1L×2
609-07474 1L×10
603-07472 MCI Buffer L-8500-PF-RG 1L×10
637-12461 1L×2
299-70501 NINHYDRIN COLORING SOLUTION KIT FOR HITACHI FOR 2L

 

(3)符合下列条件3,适合使用PF系列缓冲液 + 日立氨基酸分析仪配套茚三酮显色液
  • 仪器是L-8900型
  • 不需要高吞吐能力
  • 检测样本为生物液体
(4)符合下列条件4,适合使用PH系列缓冲液 + 日立氨基酸分析仪配套茚三酮显色液
  • 仪器是L-8900型
  • 不需要高吞吐能力
  • 检测样本为蛋白质水解产物
(5)符合下列条件5,适合使用以下配套试剂:
  • 仪器是L-8900型
  • 高吞吐能力
632-07401/PFS-KIT MCI Buffer L-8500-PF Kit
包含:PFS-1 1L*2、PFS-2 1L*2、PFS-3 1L*1、PFS-4 1L*1、PFS-RG 1L*1
1 kit
639-07411/PFS-RG MCI Buffer L-8900-PFS-1 1L×10
636-07421/PFS-4 MCI Buffer L-8900-PFS-2 1L×10
633-07431/PFS-3 MCI Buffer L-8900-PFS-3 1L×10
630-07441/PFS-2 MCI Buffer L-8900-PFS-4 1L×10
637-07451/PFS-1 MCI Buffer L-8900-PFS-RG 1L×10
299-70501 NINHYDRIN COLORING SOLUTION KIT FOR HITACHI FOR 2L

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

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Beta淀粉酶[蜡样芽胞杆菌] Beta-Amylase (B.cereus) 20K units 货号:E-BAMBC Megazyme中文站

Beta淀粉酶[蜡样芽胞杆菌]

英文名:Beta-Amylase (B.cereus) 20K units

货号:E-BAMBC

规格:20000 Units

High purity recombinant β-Amylase (Bacillus cereus) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.2
CAZy Family: GH14
CAS: 9000-91-3

beta-amylase; 4-alpha-D-glucan maltohydrolase

Recombinant. From Bacillus cereus.
In 3.2 M ammonium sulphate.

Specific activity: ~ 2,400 U/mg (40oC, pH 6.5 on soluble starch).

Stability: > 2 years at 4oC.

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Beta甘露聚糖酶(海栖热袍菌) endo-1,4-?- Mannanase (T.maritima) 货号:E-BMATM Megazyme中文站

Beta甘露聚糖酶(海栖热袍菌)

英文名:endo-1,4-?- Mannanase (T.maritima)

货号:E-BMATM

规格:250 U at 80°C

High purity endo-1,4 beta-Mannanase (T. maritima) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.78 
CAZy Family: GH5

From Thermotoga maritima. Electrophoretically homogeneous. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 14 U/mg (80oC, pH 7.0, carob galactomannan as substrate). 

Stable at 4oC for > 2 years.

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木聚糖酶[单胞杆菌] endo-1,4-β-Xylanase (Aeromonas punctata) 货号:E-XYNAP Megazyme中文站

木聚糖酶[单胞杆菌]

英文名:endo-1,4-β-Xylanase (Aeromonas punctata)

货号:E-XYNAP

规格:40 Units

High purity recombinant endo-1,4-beta-Xylanase (Aeromonas punctata) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8 
CAZy Family: GH10

Recombinant. From Aeromonas punctata. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 8.4 U/mg (40oC, pH 6.5 on wheat arabinoxylan).

Store at 4oC.

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whatman GF/F玻璃微纤维滤纸圆型 1825-047 GF/F 4.7CM 100/PK

whatman GF/F玻璃微纤维滤纸圆型 1825-047 GF/F 4.7CM 100/PK

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变异诱导剂等


产品编号 产品名称 产品规格 产品等级 产品价格
039-03851 Colchicine  ≧95.0%
 秋水仙素
100mg 和光一级
035-03853 Ethyl Methanesulfonate
 甲磺酸乙酯
1g 和光一级
325-81522 Ethyl Methanesulfonate
 甲磺酸乙酯
25g Wako
323-81523 Ethyl Methanesulfonate
 甲磺酸乙酯
100g Wako


  解析与表现型和遗传因子项链接的突变体,是识别遗传因子的有效方法。

  Ethyl Methanesulfonate(EMS)是诱变DNA醇化的烷化剂。

  Colchicine是使染色体加倍从而使染色体变异的化合物。

生化学试剂-细胞培养|试剂|关东化学株式会社

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