Kapa Biosystems官网 kapa试剂中国代理商


Kapa Biosystems官网 kapa试剂中国代理商

Kapa是世界上著名的生物技术公司。总部位于美国波士顿, 公司的主要理念是运用分子和遗传学技术来打造新一代的生物科学和医学诊断产品。
Kapa公司目前主要是在“直接进化(directed evolution technology platform)”的技术平台上来筛选和生产高品质的生物酶。目前生物医药领域所使用的大部分生物酶都只是天然存在形式,即使有些酶经过基因工程改造,但是由于酶分子自身的结构和功能限制,要想得到真正满足科研工作人员需要的产品,却是一件费时费力的事情,而且常常达不到预期的要求。“直接进化”技术正是针对这一现实情况,运用遗传,基因工程,生物信息学等技术来在分子水平上设计真正满足不同实际要求的生物酶。通过创新,Kapa为广大的科研人员提供了一系列拥有多项技术的产品,不仅能够满足普通科研需要,同时为高通量,基因芯片等高新技术的普及创造了条件。 运用“直接进化”技术,Kapa目前已经开发出来了以下的高品质产品:
1. Kapa2G超强PCR (Kapa2G Robust PCR)。适用范围:可用于普通的PCR反应中;AT和GC碱基含量高的扩增;模板 中含有常见的PCR反应抑制物,例如,盐离子,尿素,SDS,乙醇,EDTA等;石蜡油包被样品;粪便样品;菌落PCR。
2. Kapa2G 快速PCR(Kapa2G Fast PCR)。高灵敏度,高特异性。延伸速度高达1秒/kb,(常规延伸速度是60s/kb),可扩增出长达5kb的目的片段,节省大约75%的反应时间。
3.实时荧光定量PCR (Kapa SYBR qPCR)。和其他公司的类似产品相比,Kapa SYBR qPCR改造了Taq DNA多聚酶,而不是对荧光染料进行化学改造,因此大大提高了信号强度以及反应的有效性。更重要的是,Kapa的系列产品可以适用于市场上现有的Real-time PCR仪器。
4.Kapa长片段DNA多聚酶 (Kapa LongRange)。Kapa长片段DNA多聚酶是由Taq DNA聚合酶和修饰过的archael(B型)高保真DNA聚合酶组成的双酶系统。后者含有校正功能。这种双酶系统是专门为长片段和敏感PCR扩增而设计的。一般而言,普通的Taq酶由于缺少校正功能,因此随着错误碱基的不断产生,使得扩增过早的终止,从而大大降低了扩增效率。这也是导致在扩增长片段或者模板浓度很低时,使用普通Taq酶常常失败的原因。另一方面,虽然具有校正能力的多聚酶拥有很高的正确率,但由于其3到5的外切酶活性,引物会被降解掉,从而也影响到扩增的灵敏度。Kapa长片段产品的原理是:在Taq DNA多聚酶中加入具有校正能力并且修饰过的多聚酶,可以使得不匹配的碱基被修正;同时,和只有校正功能的多聚酶相比,kapa的双酶系统中具有校正功能的酶所占的比例较少,因此可以相对延长扩增的循环数和时间,从而提高产量,同时得到高保真的PCR产物。虽然两种酶都有5到3的DNA聚合功能,但是只有Taq酶具有依赖于双链的5到3的聚合酶活性,而只有B型DNA聚合酶具有3到5的外切酶功能(也就是校正功能)。
5.Kapa高保真DNA多聚酶 (Kapa Fidelity)。是经过修饰的,耐热的高保真DNA多聚酶。既有5到3的多聚酶活性,又有3到5外切校正功能,因此能够扩增出优质的高保真片段。高保真:Kapa高保真DNA多聚酶的扩增出正确片段的几率是普通Taq酶的15倍,KOD/Pfx2倍;应用广泛:可以扩增出长至6kb的质粒或者lambda DNA,或者长至2kb的基因组DNA扩增;用量少:由于其高活性,和其它的高保真产品相比,每个反应的用量要少,从而为实验者节省资金;易于克隆:Kapa高保真DNA多聚酶扩增出钝头产物,非常易于克隆;快速:扩增速度是pfu的2-3倍。 另外,还有普通的KapaTaq DNA多聚酶,以及热启动酶,T4连接酶,快速连接试剂盒等产品。

 

部分产品报价:

第二代基因工程酶及相关产品
产品归类 厂家 货号 品名 规格 目录价
HiFi高保真酶 KAPA KK2101 HiFi 100 units 700
KAPA KK2102 HiFi 250 units 1600
KAPA KK2501 HiFi Hot Start 100 units 800
KAPA KK2502 HiFi Hot Start 250 units 1800
KAPA KK2601 HiFi Hot Start 100 rxn 800
KAPA KK2602 HiFi Hot Start 500 rxn 3500
HiFi高保真酶(二代测序专用) KAPA KK2611 HiFi Hot Start for NGS 50 rxn 800
KAPA KK2612 HiFi Hot Start for NGS 250 rxn 3500
KAPA KK2701 HiFi Hot Start for NGS 50 rxn 2400
KAPA KK2702 HiFi Hot Start for NGS 250 rxn 10000
KAPA KK2801 KAPA HiFi  HS Uracil +ready Mix 50 x 50µL 3000
KAPA KK2802 KAPA HiFi  HS Uracil +ready Mix 250 x 50µL 14000
长片段酶 KAPA KK3006 Long Range 100 units 800
KAPA KK3005 Long Range 250 units 1600
KAPA KK3501 Long Range Hot Start 100 units 900
KAPA KK3502 Long Range Hot Start 250 units 1800
KAPA KK3503 Long Range Hot Start 500 units 3000
KAPA KK3601 Long Range Hot StartReady Mix with DYE  100 x 25µL 1100
KAPA KK3602 Long Range Hot StartReady Mix with DYE  500 x 25µL 4500
与takara酶相对应的KAPA Extaq酶 KAPA KK3009 KAPATaq Extra(针对takara ExTaq) 250units 400
KAPA KK3505 KAPATaq Extra Hot Start(针对takara ExTaq) 250units 520
KAPA KK3604 KAPATaq Extra Hot Start Ready Mix with DYE (针对takara ExTaq)  100 x 25µL 400
KAPA KK3605 KAPATaq Extra Hot StartReady Mix with DYE(针对takara ExTaq)  500 x 25µL 1800
KAPA KK3606 KAPATaq Extra Hot Start Ready Mix with DYE  200 x 25µL 800
HRM KAPA KK4201 KAPA HRM FAST 1ml 700
KAPA KK4202 KAPA HRM FAST 5ml 3200
KAPA KK4203 KAPA HRM FAST 10ml 6000
SYBR荧光定量 KAPA KK4600 SYBR® Fast通用型 1 ml 400
KAPA KK4601 SYBR® Fast通用型 5 ml 1800
KAPA KK4602 SYBR® Fast通用型 10 ml 3500
KAPA KK4618 SYBR® Fast通用型 50 ml 17000
KAPA KK4603 SYBR® Fast(ABI Prism) 1 ml 400
KAPA KK4604 SYBR® Fast(ABI Prism) 5 ml 1800
KAPA KK4605 SYBR® Fast(ABI Prism) 10 ml 3500
KAPA KK4617 SYBR® Fast(ABI Prism) 50ml 17000
KAPA KK4620 SYBR FAST ROX Low 500*20ul 1800
KAPA KK4606 SYBR® Fast(伯乐专用型) 1 ml 400
KAPA KK4607 SYBR® Fast(伯乐专用型) 5 ml 1800
KAPA KK4608 SYBR® Fast(伯乐专用型) 10 ml 3500
KAPA KK4609 SYBR® Fast (Roche LC 480) 1 ml 400
KAPA KK4610 SYBR® Fast(Roche LC 480) 5 ml 1800
KAPA KK4611 SYBR® Fast(Roche LC 480) 10 ml 3500
SYBR 一步法Qpcr KAPA KK4650 1-step qRT-PCR Kit(通用型) 1ml 1000
KAPA KK4651 1-step qRT-PCR Kit(通用型) 5ml 4500
KAPA KK4652 1-step qRT-PCR Kit(通用型) 10ml 8000
KAPA KK4660 1-step qRT-PCR Kit(ABI Prism) 1ml 1000
KAPA KK4661 1-step qRT-PCR Kit(ABI Prism) 5ml 4500
KAPA KK4662 1-step qRT-PCR Kit(ABI Prism) 10ml 8000
KAPA KK4670 1-step qRT-PCR Kit(伯乐专用型) 1ml 1000
KAPA KK4671 1-step qRT-PCR Kit(伯乐专用型) 5ml 4500
KAPA KK4672 1-step qRT-PCR Kit(伯乐专用型) 10ml 8000
KAPA KK4680 1-step qRT-PCR Kit(Roche LC 480) 1ml 1000
KAPA KK4681 1-step qRT-PCR Kit(Roche LC 480) 5ml 4500
KAPA KK4682 1-step qRT-PCR Kit(Roche LC 480) 10ml 8000
PROBE荧光定量 KAPA KK4701 PROBE FAST(通用型) 1 ml 400
KAPA KK4702 PROBE FAST(通用型) 5 ml 1800
KAPA KK4703 PROBE FAST(通用型) 10 ml 3500
KAPA KK4705 PROBE FAST(ABI Prism) 1 ml 400
KAPA KK4706 PROBE FAST(ABI Prism) 5 ml 1800
KAPA KK4707 PROBE FAST(ABI Prism) 10 ml 3500
KAPA KK4709 PROBE FAST(伯乐专用型) 1 ml 400
KAPA KK4710 PROBE FAST(伯乐专用型) 5 ml 1800
KAPA KK4711 PROBE FAST(伯乐专用型) 10 ml 3500
KAPA KK4714 PROBE FAST(ABI Prism,定制50ml体系) 50ml 17000
KAPA KK4715 PROBE FAST(通用型,定制50ml体系) 50ml 17000
KAPA KK4716 PROBE FAST (ROX Low Mastermix) 1ml 400
KAPA KK4717 PROBE FAST(ROX Low Mastermix) 5 ml 1800
KAPA KK4718 PROBE FAST(ROX Low Mastermix) 10 ml 3500
KAPA KK4719 PROBE FAST(ROX Low Mastermix) 50 ml 17000
KAPA KK4722 PROBE FAST Univrsal qPCR Kit with dUTP 500x20ul 1800
PROBE Force qPCR KAPA KK4752 PROBE FAST(1-step qRT-PCR Probe Universal) 5 ml 4500
KAPA KK4300 通用型PROBE FORCE定量试剂 1ml 700
KAPA KK4301 通用型PROBE FORCE定量试剂 5ml 3500
KAPA KK4302 通用型PROBE FORCE定量试剂 10ml 6000
KAPA KK4303 通用型PROBE FORCE定量试剂 50ml 26000
2G Robust酶及相关产品 KAPA KK5023  2G Robust 100 units 400
KAPA KK5024  2G Robust 250 units 900
KAPA KK5004  2G Robust(含dNTPs) 100units 400
KAPA KK5005  2G Robust(含dNTPs) 250 units 900
KAPA kk5522  2G Robust Hot Start 100units 600
KAPA KK5515  2G Robust Hot Start 250 units 1400
KAPA KK5517  2G Robust Hot Start 500 units 2400
KAPA KK5525  2G Robust Hot Start 2500units 9000
KAPA KK5532  2G Robust Hot Start(含dNTPs) 100 units 700
KAPA KK5516  2G Robust Hot Start(含dNTPs) 250 units 1500
KAPA KK5518  2G Robust Hot Start(含dNTPs) 500units 2800
KAPA KK5701  2G Robust Hot Start Readymix 100rxn 700
KAPA KK5702  2G Robust Hot Start Readymix 500 rxn 2600
2G Fast酶及相关产品 KAPA KK5523  2G Fast Hot Start 100 units 600
KAPA KK5503  2G Fast Hot Start 250 units 1300
KAPA KK5501  2G Fast Hot Start 500 units 2400
KAPA KK5519  2G Fast Hot Start 2500 units 7000
KAPA KK5530  2G Fast Hot Start(含dNTPs) 100 units 700
KAPA KK5502  2G Fast Hot Start(含dNTPs) 250 units 1500
KAPA KK5500  2G Fast Hot Start(含dNTPs) 500 units 2600
KAPA KK5513  2G Fast Hot Start(Buffer无Mg2+) 250 units 1400
KAPA KK5511  2G Fast Hot Start(Buffer无Mg2+) 500 units 2400
KAPA kk5512  2G Fast Hot Start(含dNTPs,Buffer无Mg2+) 250 units 1500
KAPA KK5510  2G Fast Hot Start(含dNTPs,Buffer无Mg2+) 500 units 2800
KAPA KK5603  2G Fast Hot Start ReadyMix 100 rxn 400
KAPA KK5601  2G Fast Hot Start ReadyMix 500 rxn 1400
KAPA KK5801  2G Fast Hot Start 100 rxn 500
KAPA KK5802  2G Fast Hot Start 500 rxn 1900

 

二代测序相关产品
厂家 货号 产品名称 规格 目录价
二代测序文库制备 KAPA KK8230 KAPA LTP文库构建(含文库扩增)试剂盒-Illumina 10个文库 2500
KAPA KK8231 KAPA LTP文库构建(不含文库扩增)试剂盒-Illumina 10个文库 2400
KAPA KK8232 KAPA LTP文库构建(含文库扩增)试剂盒-Illumina 50个文库 11000
KAPA KK8233 KAPA LTP文库构建(不含文库扩增)试剂盒-Illumina 50个文库 10500
KAPA KK8234 KAPA HTP文库构建(含文库扩增)试剂盒-Illumina 96个文库 18000
KAPA KK8235 KAPA HTP文库构建(不含文库扩增)试剂盒-Illumina 96个文库 17000
KAPA KK8260 KAPA TruSeq Adapter Kit, (Illumina, 20 μl each) 144 rxn 14000
KAPA KK2620 KAPA Library Amplification Kit with Primers (50 x 50 µl reactions) 50rxn 1800
KAPA KK2621 KAPA Library Amplification Kit with Primers (250 x 50 µl reactions) 250rxn 8000
KAPA KK2623 KAPA Library Amplification Primer Kit (250 x 50 μl rxns) 250rxn 1500
KAPA KK8400 KAPA Stranded RNA-Seq Library Preparation Kit (Illumina,24 reactions) 24 rxn 13000
KAPA KK8401 KAPA Stranded RNA-Seq Library Preparation Kit (96 reactions) 96 rxn 40000
KAPA KK8420 KAPA Stranded mRNA-Seq Library Preparation Kit (24 reactions) 24 rxn 15000
KAPA KK8421 KAPA Stranded mRNA-Seq Library Preparation Kit (96 reactions) 96 rxn 47000
KAPA KK8483 KAPA Stranded RNA-Seq Kit with RiboErase(HMR) (24rxns) 24rxn 28000
KAPA KK8484 KAPA Stranded RNA-Seq Kit with RiboErase(HMR) (96rxns) 96rxn 98000
KAPA KK8500 KAPA Hyper Prep Kit (8 reactions) 8 rxn 3100
KAPA KK8501 KAPA Hyper Prep Kit, no amplification (8 reactions) 8 rxn 2970
KAPA KK8502 KAPA Hyper Prep Kit (24 reactions) 24 rxn 8100
KAPA KK8503 KAPA Hyper Prep Kit, no amplification (24 reactions) 24 rxn 7830
KAPA KK8504 KAPA Hyper Prep Kit (96 reactions) 96 rxn 28100
KAPA KK8505 KAPA Hyper Prep Kit, no amplification (96 reactions) 96 rxn 27000
KAPA KK8510 KAPA Hyper Plus Kit (Illumina,8 rxns) 8 rxn 3500
KAPA KK8511 KAPA Hyper Plus Kit, PCR-free (Illumina,8 rxns) 8 rxn 3370
KAPA KK8512 KAPA Hyper Plus Kit (Illumina,24 rxns) 24 rxn 9300
KAPA KK8513 KAPA Hyper Plus Kit, PCR-free (Illumina,24rxns) 24 rxn 9030
KAPA KK8514 KAPA Hyper Plus Kit (Illumina,96 rxns) 96 rxn 32900
KAPA KK8515 KAPA Hyper Plus Kit, PCR-free (Illumina,96 rxns) 96 rxn 31800
KAPA KK8300 Ion Torrent  Library Preparation Kit (8 reactions) 8 rxn 2520
KAPA KK8301 Ion Torrent   Library Preparation Kit (48 reactions) 48 rxn 13860
KAPA KK8310 Ion Torrent Library Preparation Kit, no amplification (8 reactions) 8 rxn 2280
KAPA KK8311 Ion TorrentLibrary Preparation Kit, no amplification (48 reactions) 48 rxn 12420
KAPA KK8330 Adapter Kit, non-Barcoded (8 reactions    Ion Torrent 8 rxn 1200
KAPA KK8331 Adapter Kit, Barcodes 1 – 8 (48 reactions)   Ion Torrent 48 rxn 7500
KAPA KK8332 Adapter Kit, Barcodes 9 – 16 (48 reactions)   Ion Torrent 48 rxn 7500
KAPA KK8333 Adapter Kit, Barcodes 17 – 24 (48 reactions)   Ion Torrent 48 rxn 7500
二代测序文库定量:标准品+引物预混物 KAPA KK4800 DNA Quant Kit   Roche 454 FLX Each 6000
KAPA KK4801 Primer Premix  Roche 454 FLX Each 3000
KAPA KK4802 DNA Quant Kit  Roche 454 Titanium Each 6000
KAPA KK4803 Primer Premix Roche 454 Titanium Each 3000
KAPA KK4804 DNA Quant Kit  Illumina Genome Analyzer Each 6000
KAPA KK4805 Primer Premix  Illumina Genome Analyzer Each 3000
KAPA KK4806 DNA Quant Kit  ABI SOLiD Each 6000
KAPA KK4807 Primer Premix  ABI SOLiD Each 3000
KAPA KK4808 DNA Quant Kit   Illumina GA Revised Primers Each 6000
KAPA KK4809 Primer Premix  Illumina GA Revised Primers Each 3000
KAPA KK4812 DNA Quant Kit – Ion Torrent Each 6000
KAPA KK4813 DNA Quantification Primer Premix –  Ion Torrent Each 3000
KAPA KK4900 Library Quantification DNA Standards (454 FLX/Lib-A) 6 x 80 µl 4000
KAPA KK4902 Library Quantification DNA Standards (SOLiD) 6 x 80 µl 4000
KAPA KK4903 Library Quantification DNA Standards (Illumina) 6 x 80 µl 4000
KAPA KK4904 Library Quantification DNA Standards (Ion Torrent) 6 x 80 µl 4000
KAPA KK4923 KK4923Library Quant Primers+KSF(IIIumina/Uni) IIIumina/Uni 4000
KAPA KK4924 kk4924 Library Quant primers+KSF(10mTorrent/uni) 10mTorrent/uni 5000
KAPA KK4820 Library  Quant Kit,Roche 454 FLX-SYBR Fast Universal Each 7000
KAPA KK4821 Library  Quant Kit,Roche 454 Titanium-SYBR Fast Universal Each 7000
KAPA KK4824 Library  Quant Kit,Illumina GA revised primers -SYBR Fast Universal Each 7000
KAPA KK4823 Library  Quant Kit,ABI SOLiD-SYBR Fast Universal Each 7000
KAPA KK4830 Library  Quant Kit,Roche 454 FLX-SYBR Fast ABI Prism Each 7000
KAPA KK4831 Library  Quant Kit,Roche 454 Titanium-SYBR Fast ABI Prism Each 7000
KAPA KK4835 Library  Quant Kit,Illumina GA revised primers -SYBR Fast ABI Prism Each 7000
KAPA KK4833 Library  Quant Kit,ABI SOLiD-SYBR Fast ABI Prism Each 7000
KAPA KK4840 Library  Quant Kit,Roche 454 FLX-SYBR Fast Bio-Rad iCycler Each 7000
KAPA KK4841 Library  Quant Kit,Roche 454 Titanium-SYBR Fast Bio-Rad iCycler Each 7000
KAPA KK4844 Library  Quant Kit,Illumina GA revised primers-SYBR Fast Bio-Rad iCycler Each 7000
KAPA KK4843 Library  Quant Kit,ABI SOLiD-SYBR Fast Bio-Rad iCycler Each 7000
KAPA KK4850 Library  Quant Kit,Roche 454 FLX-SYBR Fast Roche Light Cycler480 Each 7000
KAPA KK4851 Library  Quant Kit,Roche 454 Titanium-SYBR Fast Roche Light Cycler 480 Each 7000
KAPA KK4854 Library  Quant Kit,Illumina GA revised primers-SYBR Fast Roche Light Cycler480 Each 7000
KAPA KK4853 Library  Quant Kit,ABI SOLiD-SYBR Fast Roche Light Cycler480 Each 7000
KAPA KK4834 Library  Quant Kit,Illumina GA ABI Prism Custom Kit- Sanger Each 7000
KAPA KK4827 Library  Quant Kit,Ion Torrent PGM universal Each 7000
KAPA KK4838 Library  Quant Kit,Ion Torrent PGM ABI Prism Each 7000
KAPA KK4847 Library  Quant Kit,Ion Torrent PGM  Bio-Rad iCycler Each 7000
KAPA KK4857 Library  Quant Kit,Ion Torrent PGM  Roche Light Cycler480 Each 7000
KAPA KK4873 KK4873Library Quant Kit (Illumina/ROX Low) 500rxn 7000
人类基因组专用定量Kit(文库构建之前用,主要检测提取的DNA量) KAPA KK4960 hg DNA Quant & QC kit with SYBR Universal qPCR Mastermix Each 8000
KAPA KK4961 hg DNA Quant & QC kit with SYBR ABI Prism qPCR Mastermix Each 8000
KAPA KK4962 hg DNA Quant & QC kit with SYBR Bio-Rad qPCR Mastermix Each 8000
KAPA KK4963 hg DNA Quant & QC kit with SYBR Light Cycler 480 qPCR Mastermix Each 8000
KAPA KK4964 hg DNA Quant & QC DNA Standards Kit Each 6000
KAPA KK4965 hg DNA Quant & QC and 41 bp Primer Kit Each 1500
KAPA KK4966 hg DNA Quant & QC and 129 bp Primer Kit Each 1500
KAPA KK4967 hg DNA Quant & QC and 305 bp Primer Kit Each 1500
KAPA KK4969 hg DNA Quant & QC Kit with ROX Low qPCR Mastermix Each 8000

海藻糖检测试剂盒Trehalose Assay Kit Trehalose Assay Kit 货号:K-TREH Megazyme中文站

海藻糖检测试剂盒Trehalose Assay Kit

英文名:Trehalose Assay Kit

货号:K-TREH

规格:100 assays (manual) / 1000 assays (microplate)

The Trehalose test kit is a simple method for the rapid and reliable measurement and analysis of trehalose in foods, beverages and other materials.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Trehalose and
D-Glucose in foodstuffs, beverages, and other materials

Principle:
(trehalase)
(1) Trehalose + H2O → D-glucose

(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1100 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 8 min
Detection limit: 37.5 mg/L
Application examples:
Honey, mushrooms, bread, beer, seafood (e.g. lobster and shrimp),
fruit juices, purees and fillings, nutrition bars, surimi, dehydrated fruits
and vegetables, fruit products, white chocolate, sports drinks, dairy
products, egg products, soups and sauces, confectionery, chewing gum,
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition: Novel method

Advantages

  • Only enzymatic kit available
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

 

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q5. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q6. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q8. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q9. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q10. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q11. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q12. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q14. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

淀粉葡萄糖苷酶[黑曲霉] Amyloglucosidase (Aspergillus niger) 货号:E-AMGDF-100ML Megazyme中文站

淀粉葡萄糖苷酶[黑曲霉]

英文名:Amyloglucosidase (Aspergillus niger)

货号:E-AMGDF-100ML

规格:326000

High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3 
CAZy Family: GH15

Amyloglucosidase.

From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Electrophoretically homogeneous. Stabilised liquid in 50% glycerol. 

Specific activity: approx. 36 U/mg (40oC, pH 4.5, soluble starch as substrate). 200 U/ml on p-nitrophenyl β-maltoside; 3,260 U/ml on soluble starch.

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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加压单元适配器120毫升CP – 300用|柴田科技有限公司-环境检测设备、科学仪器的制造销售

产品详细

科学仪器

加压单元适配器120毫升CP – 300用

CP – 300用120毫升加压容器用
加压单元适配器120毫升CP - 300用|柴田科技有限公司-环境检测设备、科学仪器的制造销售

  • 加压单元适配器120毫升CP - 300用|柴田科技有限公司-环境检测设备、科学仪器的制造销售

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产品照片 加压单元适配器120毫升CP - 300用|柴田科技有限公司-环境检测设备、科学仪器的制造销售
商品代码 054310 – 3222
型式
价格(不含税) 52万日元。

上海金畔生物科技有限公司

500%2F%3Fc%3D34& ” 500%2F%3Fc%3D34&

生物缓冲液 三羟甲基氨基甲烷 Tris 77-86-1

上海金畔生物科技有限公司提供生物缓冲液 三羟甲基氨基甲烷

缓冲溶液是一类能够抵制外界加入少量酸和碱的影响,仍能维持pH值基本不变的溶液。该溶液的这种抗pH变化的作用称为缓冲作用。缓冲溶液通常是由一或两种化合物溶于溶剂(即纯水)所得的溶液,溶液内所溶解的溶质(化合物)称之为缓冲剂,调节缓冲剂的配比即可制得不同pH的缓冲液。

在许多化学反应中,缓冲溶液被用于使溶液的pH值保持恒定。缓冲溶液对生命的产生与进化具有重要意义,因为多数生物都只能在一定pH范围内生长。缓冲液可分为四种:1. 生化缓冲液:磷酸缓冲液;2. 生物缓冲液:HEPES、MOPSO;3. 细胞培养平衡盐:Hank’s;4. 电泳缓冲液及上样缓冲液:Tris-乙酸(TAE)、Tris-磷酸(TPE)、Tris-硼酸(TBE)、Tris-甘氨酸。

缓冲溶液有许多用途,例如人体血液中含有磷酸二氢根-磷酸氢根、碳酸-碳酸氢钠等多对缓冲对,维持血液的pH在7.35至7.45之间,以维持酶的活性。在工业上,缓冲溶液常被用于调节染料的pH。缓冲溶液还可以被用于pH计的校正。

上海金畔生物科技有限公司可提供多种生物缓冲液:

 

货号 简称 英文名称 中文名称 CAS# / EC#
100130 ACES 2-[(2-Amino-2-oxoethyl)amino]ethanesulfonic acid N-(2-乙酰氨基)-2-氨基乙磺酸 7365-82-4
100181 ADA N-(2-Acetamido)iminodiacetic acid N-(2-乙酰氨基)-2-亚氨基二乙酸 26239-55-4
105121 AMP buffer 2-Amino-2-methyl-1-propanol 2-氨基-2-甲基-1-丙醇 124-68-5
105117 AMPSO 3-[(1,1-Dimethyl-2-hydroxyethyl)]amino-2-hydroxypropanesulfonic acid 3-[N-(1,1-二甲基-2-羟乙基)]氨基-2-羟丙烷磺酸 68399-79-1
111821 BES N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid N,N-双(2-羟乙基)-2-氨基乙烷磺酸 10191-18-1
111830 Bicine Bicine N,N- 二-羟乙基甘氨酸 150-25-4
184005 Bis-Tris 2,2-Bis(hydroxymethyl)-2,2′,2”-nitrilotriethanol 双(2-(羟甲基)氨基-三(羟甲基)甲烷 6976-37-0
122743 CAPS N-Cyclohexyl-3-aminopropanesulfonic acid 3-(环己氨)-1-丙磺酸 1135-40-6
122745 CAPSO 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid 3-(环己氨)-2-羟基丙磺酸 73463-39-5
122435 CHES N-Cyclohexyltaurine N-环已基-2-氨基乙磺酸 103-47-9
S19038 DIPSO 3-[N-Bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid 3-双(2-羟乙基)氨基-2-羟基丙磺酸 68399-80-4
S19039 DIPSO.Na 3-[N-Bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid.Na 3-双(2-羟乙基)氨基-2-羟基丙磺酸.钠盐 102783-62-0
142210 Glycylglycine Glycylglycine 双甘肽 556-50-3
S19040 HEPBS N-(2-Hydroxyethyl)piperazine-N'(4-butanesulfonic acid) N-(2-羟乙基)哌嗪-N’-4-丁磺酸 101308-36-7
145570 HEPES 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid N-2-羟乙基哌嗪-N-2-乙磺酸 7365-45-9
157384 HEPES. 1/2Na HEPES (Sodium salt, 1/2Na) N-(2-羟乙基)哌嗪-N-3-丙磺酸 7365-45-9
145573 HEPES.Na HEPES (Sodium salt, 1Na) N-(2-羟乙基)哌嗪-N-3-丙磺酸 103404-87-1
145575 HEPPS HEPPS N-(2-羟乙基)哌嗪-N-3-丙磺酸 75277-39-3
145577 HEPPSO Piperazine-1,4-bis(2-hydroxypropanesulfonic acid) dihydrate N-(2-羟乙基)哌嗪-N’-2-羟基丙磺酸 68189-43-5
157379 MES 2-Morpholinoethanesulfonic acid, monohydrate 2-吗啉乙磺酸 4432-31-9
157381 MOPS 3-Morpholinopropanesulfonic acid,Free Acid 3-(N-吗啉代)丙磺酸 1132-61-2
157385 MOPSO 2-Hydroxy-3-morpholinopropanesulfonic acid,Free Acid 3-(N-吗啉代)2-羟基丙磺酸 68399-77-9
157387 MOPSO.Na 2-Hydroxy-3-morpholinopropanesulfonic acid,Na  3-(N-吗啉)-2-羟基丙磺酸钠盐 79803-73-9
166741 PIPES Piperazine-1,4-bis(2-ethanesulfonic acid).Free Acid 哌嗪-N,N’-双(2-乙磺酸) 5625-37-6
166744 PIPES.2Na PIPES Disodium salt 哌嗪-N,N’-双(2-乙磺酸)二钠盐 76836-02-7
166742 POPSO Piperazine-1,4-bis(2-hydroxypropanesulfonic acid) dihydrate 哌嗪-1,4-羟基二丙磺酸 68189-43-5
184006 TAPS 3-[[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]-1-propanesulfonic acid .Free Acid N-三(羟甲基)甲基-3-氨基丙烷磺酸 29915-38-6
184007 TAPSO 2-Hydroxy-N-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid.Free Acid N-三(羟甲基)甲氨基-2-羟基丙磺酸 68399-81-5
184010 TES 2-[Tris(hydroxymethyl)methylamino]-1-ethanesulfonic acid 三(羟甲基)甲胺基乙磺酸 7365-44-8
182941 TAE Triaethanolamin-HCl 三乙醇胺-HCl 637-39-8
183994 Tris Acetate Tris(hydroxymethyl)aminomethane acetate salt 三羟甲基氨基甲烷乙酸盐 6850-28-8
183995 Tris Tris(hydroxymethyl)aminomethane 三羟甲基氨基甲烷 77-86-1
184001 Tris-HCl Tris(hydroxymethyl)aminomethane hydrochloride 三羟甲基氨基甲烷盐酸盐 1185-53-1
146181 8-Hydroxyquinoline 8-羟基喹啉 148-24-3
201294 Diethanolamine 85% 二乙醇胺 111-42-2
184030 TRICINE TRICINE 三(羟甲基)甲基甘氨酸 5704-4-1
146710 midazole Imidazole 咪唑 288-32-4

 

更多产品,更多优惠,请联系我们!

上海金畔生物科技有限公司

订货热线:15221999938

网 址: www.jinpanbio.com

金畔博客:www.jinpanbio.cn

Email:sales@jinpanbio.com

 

 

 

异潘糖 Isopanose – 100 mg 货号:O-IPAN Megazyme中文站

异潘糖

英文名:Isopanose – 100 mg

货号:O-IPAN

规格:100 mg

CAS: 82838-47-9
Molecular Formula: C18H32O16
Molecular Weight: 504.4
Purity: > 95%



High purity isopanose (6-O-α-D-maltosyl-glucosose) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis. Isopanose is formed by the action of isopullulanase on pullulan. It can be used as an analytical standard or as a substrate to help characterise the activities of other starch degrading enzymes including α-glucosidase and amyloglucosidase.

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Multigene™ optimax PCR仪 美国Labnet TC9610-230V

名称:Multigene™ optimax PCR仪

品牌:美国Labnet

订货号:TC9610-230V

Multigene™ optimax PCR仪                                                        美国Labnet                                                        TC9610-230V

咨询此产品

产品介绍

 

Multigene Optimax PCR

Labnet新款MultiGene Optimax PCR仪带来了新的运行速度和功能。这款仪器造型紧凑、价格实惠。从显示窗口可以很清晰的看到程序运行状况,仪器自身也可储存程序。该仪器还包含了2PCR标准程序,触控和时间调节非常简单。另外,PCR仪盖子的温度亦可选择。快速的温度调节能够让您在一天内完成更多的PCR流程。

此外,MultiGene Optimax PCR仪延伸并优化了旧款梯度PCR仪的梯度PCR功能,这种优良的性能可以让操作者设置6个不同的退火温度,对于优化程序和分选基因来说该功能非常适用。

产品特点

l  快速升降温速度。

l  热盖压力恒定,滑盖防止烫伤;可立便于清洁。

l  热盖2种温度范围可选。

l  全新热盖设计,高度防止冷凝水设计。

l  6个独立控制的Peltier 模块。

l  全区可分6个独立设置温度反应单元

l  Tm计算器

l  PC Viewer软件

l  图形化操作界面

l  2年的保修期

性能参数

容量

1×96孔板,12×8×0.2ml离心管,96×0.2ml离心管

程序密码保护

程序温度范围

 4-99

体积(W×D×H)

24×42×52cm

温度控制

模块

重量

9kg

温度精确度/一致性

±0.5/±0.5

变温速度

高温5℃升温/3.5℃降温

显示

 LCD 低温3℃升温/2℃降温

样品体积范围

5-100μl

电源

230V50/60Hz

热盖温度

60-65℃,100-115℃,关闭

储存程序

200

排列格式

64×4孔排列

用户文件夹

50

优化温度范围

 30-99℃,6个模块可以独立设置6个不同的退火温度,6个模块之间最大温度差不能超过24

保质期

两年

订货号 产品描述

订货号

产品描述

TC9610-230V

MultiGene OptiMaxPCR,模块:96×0.2ml

TC96-CM-10

压缩垫,硅胶,一袋10