Lumiprobe 磺酸基Cy 5活性脂 Sulfo-Cyanine 5 NHS ester

Lumiprobe 磺酸基Cy 5活性脂 Sulfo-Cyanine 5 NHS ester

水溶Cyanine 5琥珀酰亚胺酯(succinimidyl ester(SE)),类似于Cy5® NHS ester,不需要有机溶剂即在水相中即可对各种带氨基分子进行标记。因此该产品尤其适用于对那些在有机溶剂中易变性的蛋白和低溶解度蛋白的标记。
Sulfo-Cyanine 5是Cy5®的衍生物,与很多仪器如读板机(plate readers),显微镜和成像设备等相兼容。是最受欢迎的荧光染料之一。

该染料是高度亲水的、水溶的。

非磺化的衍生物:Cyanine 5 NHS ester。
可以在所有的应用中替代Cy5®,Alexa Fluor 647和DyLight 649。

一般性质
外观:深蓝色粉末
分子量:761.84
分子式:C36H40N3NaO10S2
溶解性:极易溶于水,易溶于DMF和DMSO。
质控:NMR 1H(95%)和13C,TLC,功能性测试
储存条件:储存:收到货后于-20℃避光保存24个月。运输:室温3周,避免长时间暴露于阳光,干燥。
光谱性质
最大激发波长:646
在最大激发波长下的消光系数:271000
最大发射波长:662
荧光量子产率:0.2

General properties

Appearance: dark blue powder
Molecular weight: 761.84
Molecular formula: C36H40N3NaO10S2
Solubility: very good in water, good in DMF and DMSO
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

 

Excitation maximum, nm: 646
Extinction coefficient at excitation maximum, Lmol-1cm-1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.13
CF280: 0.13

 

土壤DNA萃取的选择缓冲液


产品编号 产品名称 产品规格 产品等级 产品价格
313-06221 Lysis Solution BB SP1 
土壤DNA 萃取的选择缓冲液
50mL

土壤DNA萃取的选择缓冲液土壤DNA萃取的选择缓冲液

Lysis Solution BB SP1


◆原理


  LYSIS Solution BB SP1是是ISOIL For Beads Beating专用的选择性缓冲液。使用ISOIL For Beads Beating,在如黑土这些含水铝石英很多的土壤中萃取DNA时ISOIL For Beads Beating能够代替试剂盒附属的LYSIS Solution BB使用。使用本产品能够增加含水铝石英很多的黑土的DNA萃取量

WAKO LabAssay™ Cholestero 胆固醇定量检测试剂盒

上海金畔生物科技有限公司提供WAKO LabAssay™ Cholestero  胆固醇定量检测试剂盒

WAKO LabAssay™ Cholestero  胆固醇定量检测试剂盒

可用于人,大鼠以及小鼠血清样本的检测

(本试剂盒仅为研究试剂,不可用于诊断或做其他用途。)

LabAssay™ Cholestero  胆固醇定量检测试剂盒

【摘要】

胆固醇是生物细胞膜的主要成分,是众多动物合成甾族化合物的前体物质。

本品是利用N-乙基-N-(2-羟基-3-磺丙基)-3,5-二甲氧基苯胺钠(DAOS),通过酶浅蓝色显色反应,检测血清等样品中胆固醇量的试剂盒。与微孔板配套使用,可进行多样品检测。

【检测原理】

样品中的胆固醇酯类在胆固醇酯酶作用下,被分解为游离胆固醇和脂肪酸。在这里生成的游离胆固醇与既存的游离胆固醇类一起被胆固醇氧化酶氧化,产生过氧化氢。生成的过氧化氢,在过氧化物酶作用下,与N-乙基-N-(2-羟基-3-磺丙基)-3,5-二甲氧基苯胺钠(DAOS) 及4-氨基安替比林定量氧化缩合,产生浅蓝色色素。测量这个浅蓝色色素的吸光值,即可计算出样品中的胆固醇浓度。

294-65801 LabAssay TM胆固醇 [胆固醇氧化酶、DAOS法]

【试剂盒组成】

缓冲液—————————–150ml×2瓶(MES缓冲液)

显色剂—————————–150ml×2(溶解时:胆固醇酯酶,胆固醇氧化酶,过氧化物酶,DAOS,4-氨基安替比林,抗坏血酸氧化酶)

标准液—————————–10ml×1瓶

【性能】

灵敏度

蒸馏水作为样品时,吸光值在0.11以下。

特定浓度的标准液(200mg/dl) 作为样品时,吸光值为0.13~0.65。

特异性
可检测1,000mg/dl的总胆固醇浓度。

【检测波长】

主波长:600nm, 副波长:700nm

【试剂的配制】

显色剂:将1瓶显色剂(150mL用)溶解于1瓶缓冲液(150mL)中。

配制后,以2~10℃保存,可保存3星期。

【标准操作法】

向微孔板内添加2μl样品和300μl显色剂,充分混合,在37℃孵育5分钟。以此作为对照空白值,测量样品及标准液的吸光值。

 

产品货号 名称 包装
292-63901 LabAssay™ A/G(白蛋白与球蛋白比值检测试剂盒) 1000次
290-65901 LabAssay™ Creatinine(肌氨酸酐定量检测试剂盒) 500次
298-65701 LabAssay™ Glucose(葡萄糖定量检测试剂盒) 1000次
294-63601 LabAssay™ NEFA(游离脂肪酸定量检测试剂盒) 750次
296-63801 LabAssay™ Phospholipid(磷脂定量检测试剂盒) 1300次
290-63701 LabAssay™ Triglyceride(甘油三酯定量检测试剂盒) 1000次
291-58601 LabAssay™ ALP(碱性磷酸酶定量检测试剂盒) 900次
294-65801 LabAssay™ Cholesterol(胆固醇定量检测试剂盒) 1000次
292-64001 LabAssay™ Uric Acid(尿酸定量检测试剂盒) 1300次

 

更多产品,更多优惠,请联系我们!
上海金畔生物科技有限公司
订货热线:15221999938
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com

L-谷氨酸[谷氨酸盐/谷氨酸酯/味精/谷氨酸单钠]检测试剂盒 L-Glutamic Acid Assay Kit 货号:K-GLUT Megazyme中文站

L-谷氨酸[谷氨酸盐/谷氨酸酯/味精/谷氨酸单钠]检测试剂盒

英文名:L-Glutamic Acid Assay Kit

货号:K-GLUT

规格:60 assays (manual) /

分析物意义:常见的天然食品组分,例如在奶酪和番茄中或调味剂中,如味精 

Megazyme检测试剂盒优点:提供的硫辛酰胺脱氢酶是稳定的悬浮液而不是可溶性粉末,从而减少了酶的浪费 

The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.
Suitable for manual, auto-analyser and microplate formats.

Colourimetric method for the determination of L-Glutamic Acid
(Monosodium Glutamate; MSG) in foodstuffs and other materials

Principle:
(beef liver glutamate dehydrogenase)
(1) L-Glutamic acid + NAD+ + H2O ↔ 2-oxoglutarate + NADH + NH4+

(diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan

Kit size: 60 assays (manual) / 600 (microplate)
/ 700 (auto-analyser)
Method: Spectrophotometric at 492 nm
Reaction time: ~ 9 min
Detection limit: 0.21 mg/L
Application examples:
Fruit and vegetables (e.g. tomato), processed fruit and vegetables
(e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed
meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by ISO, GOST
and NMKL

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Glutamate dehydrogenase solution stable at -20°C
  • No wasted diaphorase solution (stable suspension supplied)
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

Q1. What level of cysteine in test samples will affect the results obtained from K-GLUT?

Samples that contain cysteine levels higher than 1 mM will not generate results within the required specification for the K-GLUT assay.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q5. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q6. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q7. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q8. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q9. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q10. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q11. Absorbance values of my sample reactions continue to increase slowly after the reaction should be complete. Is there an explanation for this?

Some samples can react with the INT in the assay and cause a non-enzymatic creep reaction.

The 3rd worksheet in the MegaCalc is used to account for any creep reaction in your results. 

Q12. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q13. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

24根用贴纸固定螺丝PEEK 6个|柴田科技有限公司-环境检测设备、科学仪器的制造销售

产品详细

科学仪器

24根用贴纸PEEK 6个固定螺丝

商品代码其他情报(式样)

这个产品比较表中追加
商品代码 F 34503 – 8494
型式
价格(不含税) 31万日元。

上海金畔生物科技有限公司

” 754%2F%3Fc%3D35 “> 754%2F%3Fc%3D35

唾液酸酶[产气英膜杆菌] exo-α-Sialidase (Clostridium perfringens) 货号:E-SIALCP Megazyme中文站

唾液酸酶[产气英膜杆菌]

英文名:exo-α-Sialidase (Clostridium perfringens)

货号:E-SIALCP

规格:5 Units

High purity recombinant exo-alpha-Sialidase (Clostridium perfringens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.18
CAZy Family: GH33

Recombinant. From Clostridium perfringens. In 3.2 M ammonium sulphate. Hydrolysis of unbranched, non-reducing terminal α-2,3-linked, α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates.

Specific activity: ~ 140 U/mg (37oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid)

Store at 4oC.

Custom quantities and formulations available up on request.

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Stemfull低吸附15mL离心管


产品编号 产品名称 产品规格 产品等级 产品价格
MS-90150Z Stemfull低吸附15mL离心管 100个

Stemfull低吸附15mL离心管Stemfull低吸附15mL离心管

Stemfull低吸附15mL离心管

◆优点特色

 对干细胞等吸附性强的细胞株可发挥极大的效果.

● 基质表面经稳定的化学结合表面处理,不会溶出处理液。

● 透明度高,可进行高效离心分离和回收操作。

 本产品已使用放射线灭菌。

产品编号

产品名称

材质

容量

包装

MS-90150Z

Stemfull低吸附15mL离心管

管:PET   盖:聚乙烯

15mL

500包/箱