HTL移液器系列 Discovery系列移液器

  • HTL简介:
    波兰High Tech Lab公司生产高质量实验及临床仪器、耗材产品。该公司由两部分组成:位于Warsaw的PZ HTL S.A.生产精密测量产品;位于Ozorkow的HTL-STREFA Sp.zo.o.生产实验室耗材及外科刀具。HTL产品通过了ISO9001质量体系认证,其中HTL Strefa Sp.zo.o.生产的医学产品符合欧盟Directive 93/42/EEC标准、并携带有CE标志,为美国食品与药物管理局(FDA)注册产品。
    HTL有20多年的精密移液器设计、生产经验,是世界上20多个品牌的移液器OEM工厂,HTL自有品牌的移液器更是在自己独有的制造水平之上博采众长,成为世界具有世界顶级品质的产品。DISCOVERY系列移液器更是集HTL公司20多年制造经验之精华的扛鼎之作。
    内容介绍

    Discovery系列移液器特点:
    1、 单道移液器和多道移液器均可以整支高温消毒。(121℃0.1MPa条件下消毒20分种)。10次灭菌内无需校准。
    2、 人体工程学的精湛设计,自然地握持手柄和操作,无需大力。无论是设定容量,移液或是排卸吸嘴,整个过程动作顺畅,适合每天进行的日常工作。
    3、 单道移液器和多道移液器容量调整由原来的一点调整改为二点调整,避免在调整容量过程中夹掩手套。揿钮有左右各3mm的自由行程,吸、排液时不会改变已设定吸取量。
    4、 多道移液器吸嘴套筒可以伸缩,这样可使各个通道受力均匀,安装吸嘴时不用左右摇晃,即可保证各个吸嘴均匀的安装在移液器上。
    5、 1000uL以上量程移液器活塞采用特殊陶瓷材料制成,可抗腐蚀,耐酸碱,延长移液器活塞使用寿命。
    6、 弹射器设计长短可调(见右图),不仅使移液器兼容不同品牌的吸嘴,也使排卸吸嘴时格外轻松。大容量移液器(DV5000及DV10000)也同样装有弹射器。—每支移液器均随包装附有3只内部长短不一的H型弹射器适配头,通过更换适配头来改变弹射器的整体长度。
    7、 每支移液器均配有支架及校准工具,用户可以在实验室自行校准移液器。
    8、 更加符合GLP规范,吸液按扭及弹射器按钮都有明显色标,与型号及备用的吸嘴相符。
    9、 HTL移液器任何一个细小的零部件均由波兰HTL公司原厂生产。
    10、各种型号的移液器均通过ISO9001质量认证,并提供 3年质量保证。
    移液器的精确度和精密度除了与移液器自身品质相关外,还和使用的吸头的品质有关。下表是Discovery移液器的吸头配套参数

     

    型号
    目录号
    测试量(ul)
    精确度 (%)
    精密度 (%)
    适用吸头
    DV2
    0.1-2ul
    4301
    0.2
    ±12.0
    ±6.0
    10ul
    1.0
    ±2.7
    ±1.3
    Max
    2.0
    ±1.5
    ±0.7
    DV10
    0.5-10ul
    4302
    Min
    0.5
    ±4.0
    ±4.0
    5.0
    ±1.0
    ±0.8
    Max
    10.0
    ±0.5
    ±0.4
    DV20
    2-20ul
    4303
    Min
    2
    ±3.0
    ±1.5
    200ul
    10
    ±1.0
    ±0.7
    Max
    20
    ±0.8
    ±0.3
    DV50
    5-50ul
    4307
    Min
    5
    ±2.5
    ±2.0
    25
    ±1.0
    ±0.6
    Max
    50
    ±0.8
    ±0.4
    DV100
    10-100ul
    4304
    Min
    10
    ±1.6
    ±0.8
    50
    ±1.0
    ±0.4
    Max
    100
    ±0.8
    ±0.3
    DV200
    20-200ul
    4305
    Min
    20
    ±1.2
    ±0.6
    100
    ±1.0
    ±0.3
    Max
    200
    ±0.6
    ±0.2
    DV250
    50-250ul
    4300
    Min
    50
    ±1
    ±0.4
    300ul
    125
    ±0.8
    ±0.3
    Max
    250
    ±0.6
    ±0.3
    DV1000
    200-1000ul
    4306
    200
    ±0.8
    ±0.4
    1000ul
    500
    ±0.7
    ±0.25
    Max
    1000
    ±0.6
    ±0.15
    DV5000
    1000-5000ul
    4308
    Min
    1000
    ±0.6
    ±0.3
    5000ul
    2500
    ±0.6
    ±0.3
    Max
    5000
    ±0.5
    ±0.2
    DV10000
    1000-10000ul
    4309
    Min
    1000
    ±2.5
    ±0.6
    10000ul

     

    订货信息

     

    产品编号 产品内容 规格
    4301 单道可调精密移液器 0.1-2ul
    4302 单道连续可调精密移液器 0.5-10ul
    4303 单道连续可调精密移液器 2-20ul
    4307 单道连续可调精密移液器 5-50ul
    4304 单道连续可调精密移液器 10-100ul
    4305 单道连续可调精密移液器 20-200ul
    4300 单道连续可调精密移液器 50-250ul
    4306 单道连续可调精密移液器 100-1000ul
    4308 单道连续可调精密移液器 1-5ml
    4309 单道连续可调精密移液器 1-10ml
    5221 8道连续可调精密移液器 1-10ul
    5222 8道连续可调精密移液器 5-50ul
    5223 8道连续可调精密移液器 20-200ul
    5224 8道连续可调精密移液器 50-300ul
    5225 12道连续可调精密移液器 1-10ul
    5226 12道连续可调精密移液器 5-, 50ul
    5227 12道连续可调精密移液器 20-200ul
    5228 12道连续可调精密移液器 50-300ul
    20 连续加样器MiniLab201 0.05-50ml
    302 移液管电动助力器SwiftPet 0.1-100ml

     

D-3羟丁酸检测试剂盒 D-3-Hydroxybutyric Acid 货号:K-HDBA Megazyme中文站

D-3羟丁酸检测试剂盒

英文名:D-3-Hydroxybutyric Acid

货号:K-HDBA

规格:60 assays (manual) /

The D-3-Hydroxybutyric Acid Assay Kit is suitable for the specific measurement and analysis of D-hydroxybutyric acid in eggs and egg products and other foods and beverages. Suitable for manual, auto-analyser and microplate formats.

For the rapid and specific assay of D-lactic acid in wine, beer, juice, milk, cheese, vinegar and meat products. Content:50 assays per kit

 

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction (~ 3 min)
  • No wasted diaphorase solution (stable suspension supplied)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

Colourimetric method for the determination of D-3-Hydroxybutyric Acid in foodstuffs

Principle:
(3-hydroxybutyrate dehydrogenase)
(1) D-3-Hydroxybutyrate + NAD+ ↔ acetoacetate + NADH + H+

(diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan

Kit size: 60 assays (manual) / 600 (microplate)
/ 740 (auto-analyser)
Method: Spectrophotometric at 492 nm
Reaction time: ~ 3 min
Detection limit: 0.20 mg/L
Application examples:
Egg, egg products (e.g. egg powder) and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by CEC

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q3. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q4. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q5. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q6. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q7. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q8. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q9. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q10. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q11. Absorbance values of my sample reactions continue to increase slowly after the reaction should be complete. Is there an explanation for this?

Some samples can react with the INT in the assay and cause a non-enzymatic creep reaction.

The 3rd worksheet in the MegaCalc is used to account for any creep reaction in your results. 

Q12. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q13. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

兽药混合标准液(激素类)


产品编号 产品名称 产品规格 产品等级 产品价格
220-02203 Veterinary Drug Mixture Standard Solution (Hormone) (each 20μg/ml Acetonitrile Solution) 
兽药混合标准液(激素类)(各20μg/ml乙腈溶液)
1ml for High Performance Liquid Chromatography
224-02201 Veterinary Drug Mixture Standard Solution (Hormone) (each 20μg/ml Acetonitrile Solution)
 兽药混合标准溶液(激素类)(各20μg/ml)
1mlx5A for High Performance Liquid Chromatography

兽药混合标准液(激素类)

Veterinary Drug Mixture Standard Solution (Hormone) (each 20μg/ml Acetonitrile Solution)

 

◆成分

氯司替勃,玉米赤霉醇,泼尼松龙,α-群勃龙,β-群勃龙,甲泼尼龙

每个成分浓度均为20 ug/mL,溶剂: 乙腈;

 

相关产品


产品编号

产品名

中文名

包装

220-01681

Veterinary   Drug Mixture Standard Solution PL-1-3 (each 20μg/ml   Methanol Solution)

兽药混合标准液PL-1-3(各20μg/ml甲醇溶液)

1ml x 5A

226-01683

1ml

227-02051

Veterinary   Drug Mixture Standard Solution (Quinolone) (each 20μg/ml)

兽药混合标准液(喹诺酮类药物)(各20μg/ml)

1ml

223-02053

1ml x5A

224-02083

Veterinary   Drug Mixture Standard Solution (Sulfonamide+Antifolate) (each 20μg/ml)

兽药混合标准溶液(磺胺类+抗叶酸制剂类)(各20μg/ml)

1ml x5A

228-02081

1ml

224-02103

Veterinary   Drug Mixture Standard Solution (Dye) (each 20μg/ml   Methanol Solution)

兽药混合标准溶液(染料类)(各20μg/ml)

1ml x5A

228-02101

1ml

225-02091

Veterinary   Drug Mixture Standard Solution (Macrolide) (each 20μg/ml Acetonitrile Sol

兽药混合标准液(大环内酯类)(各20μg/ml乙腈溶液)

1ml

221-02093

1ml x5A

223-01693

Veterinary   Drug Mixture Standard Solution PL-2-1 (each 20μg/ml   Methanol Solution)

兽药混合标准液PL-2-1(各20μg/ml甲醇溶液)

1ml

227-01691

Veterinary   Drug Mixture Standard Solution PL-2-1 (each 20μg/ml   Methanol Solution)

兽药混合标准液PL-2-1(各20μg/ml甲醇溶液)

1ml x5A

生长调节剂等


产品编号 产品名称 产品规格 产品等级 产品价格
359-20561 1,2-O-Isopropylidene-α-D(-)-xylofuranose  ≧97.0%
 1,2-O-异亚丙基-alpha-D-呋喃木糖
5g Wako
357-20562 1,2-O-Isopropylidene-α-D(-)-xylofuranose  ≧97.0%
 1,2-O-异亚丙基-alpha-D-呋喃木糖
25g Wako
056-05661 Ergosterol ≧85.0%
 麦角固醇
1g 化学用
052-05663 Ergosterol ≧85.0%
 麦角固醇
5g 化学用
054-05662 Ergosterol ≧85.0%
 麦角固醇
25g 化学用
135-14411 Methyl Jasmonate  ≧85.0%
 茉莉酮酸甲酯 
5mL 生化学用
133-14412 Methyl Jasmonate  ≧85.0%
 茉莉酮酸甲酯 
25mL 生化学用
201-20171 1-Triacontanol 100mg 植物组织培养用
306-02871 Brassicasterol  ≧97.0%
 菜籽甾醇
100mg Tama Biochemical
322-56502 Phloroglucinol (Phloroglucinol, Anhydrous) 25g Wako
326-56505 Phloroglucinol (Phloroglucinol, Anhydrous) 500g Wako
CEP1(C-Terminally Encoded Peptide)
332-44871 CEP1(C-Terminally Encoded Peptide) 0.1mg
Phytosulfokine
337-32091 Z-Ala-Ala-Asn-MCA 5mg
335-32271 Ac-Glu-Ser-Glu-Asn-MCA 5mg

生长调节剂

 

产品编号

产品名称

规格

容量

359-20561

1,2-O-Isopropylidene-α-D(-)-xylofuranose  ≧97.0%
  1,2-O-异亚丙基-alpha-D-呋喃木糖

Wako

5g

357-20562

25g

056-05661

Ergosterol
  ≧85.0%
  麦角固醇

化学用

1g

052-05663

5g

054-05662

25g

135-14411

Methyl Jasmonate  ≧85.0%
  茉莉酮酸甲酯

生化学用

5mL

133-14412

25mL

201-20171

1-Triacontanol

植物组织培养用

100mg

306-02871

Brassicasterol  ≧97.0%<br>
  菜籽甾醇

Tama Biochemical

100mg

322-56502

Phloroglucinol (Phloroglucinol,   Anhydrous)

Wako

25g

326-56505

500g

 


■CEP(C-Terminally Encoded Peptide)


促进植物氮素吸收的新肽键

  近年来,分泌型短肽在植物形态的形成和环境应答方面的重要作用已经逐渐显著1)。C-Terminally Encoded Peptide(CEP1)是分泌型短肽之一,在植物体中担任抑制氮素吸收的作用2)。

  植物从其根部将CEP1吸收到体内,使植物体内硝酸运转量成倍增长,从而促进硝酸因子的吸收3)。

[参考文献]:

 1) Annu.Rev.Plant Biol.,65,385(2014).

 2) Plant J.,55,152(2008).

 3) Science,346,343(2014).


产品编号

产品名称

规格

容量

332-44871

CEP1(C-Terminally Encoded Peptide)

0.1mg

 

 

■Phytosulfokine


含硫酸化酪氨酸的植物生长激素

  1996年,名古屋大学的松林、坂神先生从芦笋培养基中分离发现了世界上第一个肽类植物激素——Phytosulfokine1)。其分子内2个残留物翻译后会受硫酸化,这个硫酸化过程是生理活性的所必须的。

  Phytosulfokine对植物体的1)纳摩尔浓度下对植物细胞的增殖、分化活性;2)叶绿素合成促进活性;3)不定根、芽的形成;4)不定胚的形成;5)抗病害性等方面都起着相关作用2-5)。

[参考文献]:

 1) Y.Matsubayasi and Y.Sakagami,Proc.Natl.Acad.Sci.U.S.A.,93,7623(1996).

 2) Y.Matsubayasi,et al.,Chem.Record.,6.356(2006).

 3) A.Bahyrycz,et al.,j.Pept.Sci.,13,787(2007).

 4) D.Igarashi,et al.,Plant J.,71,194(2012).

 5) N.Sthrwohldt,et al.,PLoS,6,e21054(2011).


产品编号

产品名称

规格

容量

337-32091

Z-Ala-Ala-Asn-MCA

5mg

335-32271

Ac-Glu-Ser-Glu-Asn-MCA

5mg

 

生长调节剂等

植物研究用试剂

抗Bcl-xL抗体[ E18 ](ab32370)

种属反应性

与反应:小鼠、大鼠、人预测可用于:抗Bcl-xL抗体[ E18 ](ab32370)
  • 应用 AB评论 说明 WB 1 / 1000。检测到的频带的约26 kDa。 ihc-p 2000 / 1。

    见协议(链路:http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol)。

    国际商会/如果 1 / 100 – 1 / 500。

     

    流式细胞 1 / 20 – 1 / 100。

    ab172730兔单克隆抗体,适用于该抗体的同型对照。

    IP 1 / 10 – 1 / 30。

     

  • 靶标

    • 功能细胞死亡的强效抑制剂。抑制活化半胱天冬酶(相似性)。似乎通过阻断电压依赖性离子通道调节细胞死亡(VDAC)结合,阻止caspase激活,CYC1的释放,从线粒体膜。亚型基因(S)促进细胞凋亡。
    • 组织特异性Bcl-x(S)是在细胞进行高周转率的高水平表达,如发展淋巴细胞。与此相反,Bcl-X(L)在含长寿的有丝分裂后的细胞组织,如成人的大脑。
    • 序列相似性属于Bcl-2家族。
    • 结构域BH4的主题是抗凋亡活性所必需的。的BH1和BH2基序是两异与其他Bcl-2家族成员和细胞死亡的抑制要求。
    • 翻译后修饰蛋白水解裂解的半胱天冬酶在细胞凋亡。裂解蛋白,缺乏BH4的主题,具有促凋亡活性。
    • 细胞定位线粒体膜。核膜。线粒体膜和核信封。
    • 以上信息来自:UniProt加入目标q07817UniProt协会通用蛋白质资源(UniProt)2010
      核酸研究38(2010):d142-d148

      该文章由WP-AutoPost插件自动采集发布

      原文地址:http://translate.baiducontent.com/transpage?cb=translateCallback&ie=utf8&source=url&query=http%3A%2F%2Fwww.abcam.cn%2Fbcl-xl-antibody-e18-ab32370.html&from=en&to=zh&token=&monLang=zh

    ISOGENOME


    产品编号 产品名称 产品规格 产品等级 产品价格
    318-08111 ISOGENOME 10 mL
    314-08113 ISOGENOME 100 mL

    ISOGENOME

      ISOGENOME用于从组织及细胞提取基因组DNA。

      本产品为含有胍及表面活性剂的溶液。加水分解RNA即可选择性回收DNA。操作简单,便于大量提取及进行多个样品的处理。此外,配合RNA提取用试剂 ISOGEN II使用,可从同一样本提取RNA和DNA。

      往样品添加ISOGENOME,进行溶解或均匀化,再进行乙醇沉淀、乙醇清洗、溶解,可快速提取DNA。

    ◆特点

      ● 提取基因组DNA仅需30分钟左右

      ● 新鲜组织的基因组回收率达70%以上

      ● 配合ISOGEN II使用可提取RNA及DNA

      ● 产品不含苯酚,操作中亦无须使用

      ● 提取到的DNA可直接应用于各种实验 Southernblotting、斑点杂交dot-blot hybridization、

               克隆、PCR等

    ◆使用实例

    操作前的注意事项

      ● 除了本产品,需准备乙醇(99.5%)、75%乙醇、8 mM NaOH、1M HEPES(用于调整pH)。
      ● 如需使用1.5 mL容量的离心管,步骤所用溶液请按比例缩小到0.8倍。
      ● 可在室温进行全程操作,但建议于4~28°C环境下进行离心操作。
      ● 高分子的基因组DNA容易被切断,混合时应避免过于激烈的震荡或漩涡混合,应尽量平稳地进行充分的混

              合。

    操作流程A:分离基因组DNA

    ISOGENOME

    操作流程B:配合使用ISOGEN II分离RNA、DNA(简易操作流程)

    ISOGENOME

    Data 1 DNA回收量标准

     样品

    µg DNA/mg   tissue

    肝脏

    4.3 ± 0.7

    肾脏

    3.6 ± 0.2

    脾脏

    22.8 ± 4.1

    心脏

    2.0 ± 0.4

    2.5 ± 0.4

    骨骼肌

    0.8

      一般情况下,分离后的基因组DNA纯度(A260/A280)为1.6~1.9,大小为20~100 kbp。

     ◆产品内容

    ISOGENOME (10 mL、100 mL)

    产品

    包装

    保存

    备注

    ISOGENOME

    1瓶

    室温

    外观:绿色溶液
    容器:塑料瓶

     备注 本产品若长时间的低温保存,可能会析出少量的盐,但是不影响品质及性能。若出现该情况,请对各容器进行55~65°C、30~60分钟左右的孵化,并适当混合,待析出物完全溶解后即可使用。

     

    ◆备注

     本产品仅适用于实验研究。请勿用于医疗用途

    ◆相关产品

    · ISOGEN II RNA提取试剂

    · Ethachinmate 酒精沉淀共沉淀剂

    · TE (pH8.0)

    · Distilled Water, Deionized, Sterile 去离子蒸馏水

    开放系统KOACH清洁(教练)|开放清洁系统(兴研株式会社)|生化学相关设备|试剂|关东化学株式会社

    我们是兴研股份公司制的开放清洁系统KOACH(教练)取扱っ。KOACH新想法产生的保洁区域生成装置。“围墙KOACH”,尽管广范围的清洁空间可以形成。所有的“KOACH”,在研究室里也在生产现场也可以选择不设置场所ISO班1的清洁度,实现了。开关的105秒后*在那里ISO班1超级清洁区出现。*开放清洁长椅「KOACH C 900 – F」的场合

    开放系统KOACH清洁(教练)|开放清洁系统(兴研株式会社)|生化学相关设备|试剂|关东化学株式会社


    桌子教练「KOACH T 500 – F”(FERENA搭载)

    清洁度:ISO班1 /全唤起时间:110秒(设定风速0.4 m / sec点)

    开放系统KOACH清洁(教练)|开放清洁系统(兴研株式会社)|生化学相关设备|试剂|关东化学株式会社

    桌子上清净化。简单持ち運ん设置紧凑的尺寸。 机体外形尺寸:W 541毫米×D 289毫米×H 334毫米吹出开口面尺寸:w 494毫米×H 306毫米清洁空间:700毫米以内*1(开口面间距离)重量:约14公斤/台电来源:单相100V 50Hz / 60Hz额定功率*2: 35 ~ 110 W /台* 1 .设定风速0.4 m / sec时
    * 2 .滤波器造成的压力损失,及设定风速伴随变动。

    开放清洁长椅「KOACH C 900 – F”(FERENA搭载)

    清洁度:ISO班1 /全唤起时间:105秒

    开放系统KOACH清洁(教练)|开放清洁系统(兴研株式会社)|生化学相关设备|试剂|关东化学株式会社桌子上的作业区域的清洁化如果想要适合,并且排列作业,见面工作也可以。 机体外形尺寸:W 1,003毫米×D 340毫米×1,H 625毫米吹出开口面尺寸:w 900毫米×H 700毫米清洁空间:2,300毫米以内(开口面间距离)重量:约118公斤/台电来源:单相100V 50Hz / 60Hz额定功率*:360 ~ 550 W /台*滤波器的压力损失伴随变动。

    连续教练「KOACH R 1050 – F”(FERENA搭载)

    清洁度:ISO班1 /全唤起时间:130秒

    开放系统KOACH清洁(教练)|开放清洁系统(兴研株式会社)|生化学相关设备|试剂|关东化学株式会社横向排列的事尽管清洁空间横向展开。 机体外形尺寸:W 1,053毫米×D 410毫米×1,H 625毫米
    吹出开口面尺寸:w 1,简体中文约050毫米×H 700毫米(1台)清洁空间:2,400毫米以内(开口面间距离)重量:约128 kg /台电来源:3相200V 50Hz / 60Hz额定功率*:395~770 W /台*滤波器的压力损失伴随变动。

    フロアー教练「KOACH F 1050 – F”(FERENA搭载)

    清洁度:ISO班1 /全唤起时间:130秒

    开放系统KOACH清洁(教练)|开放清洁系统(兴研株式会社)|生化学相关设备|试剂|关东化学株式会社连续类型“KOACH”的技术的应用,并且风机背面移动的事,从地板的整个地区清洁化。复数台组合相向的话那么小,一边清洁室的诞生。 机体外形尺寸:W 1,055毫米×D 627毫米×H 929毫米
    吹出开口面尺寸:w 1,简体中文约050毫米×H 850毫米(1台)清洁空间:4,1000毫米以内(4组套的情况)(开口面间距离)重量:大约136 kg /台电来源:3相200V 50Hz / 60Hz额定功率*:630万~1,W /台*滤波器的压力损失伴随变动。

    フロアー教练「KOACH Ex – F”(FERENA搭载)

    清洁度:ISO班1 /施工完毕时:0.1μm(10个/ m3全换气时间:360秒(3段×10列,设定风速:0.3 m / sec点)

    开放系统KOACH清洁(教练)|开放清洁系统(兴研株式会社)|生化学相关设备|试剂|关东化学株式会社食品前方隧道型的导游屏幕AC380V大空间的超级洁净室诞生了。

    吹出开口面尺寸:w 1,简体中文约050毫米×H 850毫米(1台)清洁空间:40米以内(3段×10列的情况)(开口面间距离)电来源:3相200V 50Hz / 60Hz额定功率*:189~1万台,W /*滤波器的压力损失及设定风速伴随变动。

    产品信息

    产品名 产品编号 厂商编号 备注
    桌子教练KOACH T 500 – F 49785 – 04 901075 详细情况请咨询
    开放清洁长椅KOACH C 900 – F 49785 – 01 901024 详细情况请咨询
    连续教练KOACH R 1050 – F 49785 – 03 901059 详细情况请咨询
    フロアー教练KOACH F 1050 – F 49785 – 41 901040 详细情况请咨询

    上述系列以外的各种各样类型的通风设备齐全。详细情况请咨询。

    的相关产品

    phi 29 DNAポリメラーゼセット
    电子相关材料试剂

    试剂的咨询

    信息查询

    电话: 03 – 6214 – 1090

    传真: 03 – 3241 – 1047

    该文章由WP-AutoPost插件自动采集发布

    原文地址:http://translate.baiducontent.com/transpage?cb=translateCallback&ie=utf8&source=url&query=http%3A%2F%2Fwww.kanto.co.jp%2Fproducts%2Fsiyaku%2Fbio_equipment%2Fclean_zone_creator%2Fkoken_koach.html&from=jp&to=zh&token=&monLang=zh

    日本三菱MGC AnaeroPack 系列产品厌氧培养罐、厌氧产气袋、微需氧产气袋

    厌氧罐-日本三菱(MGC AnaeroPack Series)
    三菱瓦斯化学株式会社(MITSUBISHI GAS CHEMICAL COMPANY, INC.)长期致力于脱氧剂研究,在世界上率先开发出zui简便、zui经济的厌氧培养系列产品,广泛适用于医院临床检验、食品卫生检疫、啤酒检测监控、微生物科研等行业进行特殊微生物的培养。目前,MGC AnaeroPack 系列产品有厌氧培养、微需氧培养、二氧化碳培养三大类,2.5升和350毫升两个系列以及配套的密封培养罐、培养袋和氧气指示剂。
    厌氧罐-日本三菱(MGC AnaeroPack Series)产品特点:
    操作简便 不用加水、不用催化剂,只需把药剂(产气袋)外袋剪开放入容器内,密封后即可形成适宜的厌氧、微需氧、二氧化碳培养环境。
    适用广泛 培养容器内不会产生压差,不会产生高温。同型号的培养容器可通用,废弃物处理方便,不污染环境。
    高效经济 无须使用昂贵且占空间的大型设备,无须使用危险气体,性能价格比更经济实惠。
    针对性强 厌氧、微需氧、二氧化碳系列产品,根据培养量选择适合的容器,产气量稳定准确有保障。
    订购信息:
    品名 编号 包装规格        用途、配套及培养量
     培养容器
    2.5L密封培养罐 C-31 1只/包 19.7×13.5×9.5cm;使用2.5L系列产气袋1只,2.5升≤10皿
    7.0L密封培养罐 C-32 1只/包 28.0×21.3×11.2cm;使用2.5L系列产气袋3只,7.0升≤30皿
    15×30cm培养袋 C-41 10只/包 自带密封压槽,重复使用;使用350ml产气袋1只,2-4皿
     2.5升系列产气袋(AnaeroPack)
    厌氧产气袋 C-1 10只/包 完全厌氧菌培养;适用于2.5L或7L密封培养罐
    微需氧产气袋 C-2 10只/包 微需氧菌培养;适用于2.5L或7L密封培养罐
    二氧化碳产气袋 C-3 10只/包 嗜二氧化碳菌培养;适用于2.5L或7L密封培养罐
     350毫升系列产气袋(AnaeroPouch)
    厌氧产气袋 C-11 10只/包 完全厌氧菌培养;适用于15×30cm培养袋
    微需氧产气袋 C-12 10只/包 微需氧菌培养;适用于15×30cm培养袋
    二氧化碳产气袋 C-13 10只/包 嗜二氧化碳菌培养;适用于15×30cm培养袋
     氧气指示剂(Oxygen Indicator)
    氧气指示剂 C-22 10只/包 厌氧培养监测专用;1只/次,可重复使用4-5次

    技术指标:
      2.5L密封培养罐:2.5L密封罐:规格尺寸:19.7×13.5×9.5cm ,体积:2.5LL。罐体材料:Polycarbonate 可耐受温度:-50℃~140℃密封材料:Polyacetal 可耐受温度:-30℃~140℃ 外观透明,可分别放置12只标准培养皿(直径:9cm)
      7.0L密封培养罐:7.0L密封罐:规格尺寸:21.3×28.0×11.2 cm,体积: 7.0L。罐体材料:Polycarbonate 可耐受温度:-50℃~140℃密封材料:Polyacetal 可耐受温度:-30℃~140℃ 外观透明,可分别放置42只标准培养皿(直径:9cm)
    AnaeroPack(安宁包)
      厌氧产气袋:专用于2.5L或以上体积密封罐,药剂放入容器密封后,容器内的氧气在0.5~1小时内被完全吸收,同时产生等量的二氧化碳,适合厌氧菌培养。
      微需氧产气袋:药剂放入容器密封后,容器内的氧气在0.5~1小时内被吸收一半,同时产生等量的二氧化碳,O2及CO2浓度变为10%左右,适合微需氧菌培养。
      培养基保存袋:自带封口条,外观透明,易于操作,易于观察。zui适用于少量样品的培养。
      CO2产气袋:药剂放入容器密封后,容器内的氧气部分吸收,浓度变为15%左右,二氧化碳浓度变为6%左右,适合嗜二氧化碳菌培养。
      氧气指示剂:无氧状态(O2<0.1%):浅紫色到粉红色;有氧状态(O2>0.5%):淡蓝色到深蓝色。用于检测氧气的存在,可重复使用次4-5。

    厌氧产气袋培养对象例名称说明应用行业
    厌氧
    (0%氧气)
    肉毒杆菌Clostridium Botulinum严重致病菌,引起食物中毒食品安全,疾病预防,药企微生物检查
    产气荚膜梭菌Clostridium Perfringens临床致病菌临床检查科室,药企微生物检查
    破伤风梭菌C.tetani引起破伤风的病原菌临床检查科室,药企微生物检查
    艰难梭菌C.difficile临床致病菌检验检疫,检查科室,药企微生物检查
    微需氧
    (氧气浓度3-15%)
    弯曲杆菌属Campylobacter肠道感染病原菌食品安全,检验检疫,临床应用,药企微生物检查
    螺旋杆菌Helicobacter pylori
    二氧化碳
    (二氧化碳浓度5%)
    淋病奈瑟氏菌Neisseria gonorrhoeae临床致病菌临床应用,药企微生物检查
    肺炎链球菌Streptococcus pneumoniae
    布鲁氏菌Brucella