甘油检测试剂盒 Glycerol Assay Kit 货号:K-GCROL Megazyme中文站

甘油检测试剂盒

英文名:Glycerol Assay Kit

货号:K-GCROL

规格:70 assays (manual) / 700 assays (microplate)

分析物意义:常见食品组分,或作为甜味剂,或用于改善口感

Megazyme检测试剂盒优点:新型的药片模式,性质更稳定,反应快

The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+

Kit size: 70 assays (manual) / 700 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.34 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by OIV and
MEBAK

Advantages

  • Novel tablet format for increased stability
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual and microplate formats

 

 Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q2. Is K-GCROL specific for glycerol?

K-GCROL is highly specific for glycerol.
Some compounds that are known not to react or interfere with the assay include:
Polyethylene glycol
Ethylene glycol  
Propylene glycol 

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q5. Is the Glycerol Assay Kit (K-GCROL) suitable for measurement using cell culture media samples?

Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit.  It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed, by dilution (if required) in distilled water. 

Q6. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q7. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q8. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q9. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q10. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q11. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q12. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q13. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q14. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q15. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q16. To measure fermentation samples contain high microbial cell density, is cell disruption required?

Cell disruption is only require to measure glycerol within microbial cells.

To measure glycerol in the extracellular media only then cell disruption is not required and centrifugation of the sample may be sufficient, e.g.:

(a) Determination of glycerol in cell culture media/supernatants. In general, the concentration of glycerol in cell culture media/supernatants can be determined without any sample treatment (except clarification by centrifugation/filtering or dilution according to the dilution table, if necessary). Typically, no clarification or dilution is required, and a sample volume of 0.1 mL is satisfactory.

If interference is suspected then sample clarification/deproteinisation using carrez reagents or perchloric acid should be used (methods are provided in the kit booklet).

Pall颇尔Nanosep®和Nanosep MF离心管

Pall颇尔Nanosep®和Nanosep MF离心管

快速处理样本。

—通常,回收率高于90%;
—备有低蛋白质结合的Omega™、Bio-Inert®或GHP滤膜可供选择。
—对应各种截留分子量,备有各种编码以供轻松识别的颜色可供选择。
—结构材料为低结合聚丙烯。
—超声焊接密封,防止侧流或密封不严。
—适用于配合1.5mL试管的标准离心转子

PALL Nanosep®和Nanosep MF离心管

应用

  • 对多肽和蛋白质进行浓缩、提纯和除盐操作。
  • 从丙烯酰胺凝胶中分离蛋白质。

准备HPLC分析样本。

技术参数

能够在5到15分钟内对50到500µl的样本进行简单可靠的浓缩和除盐操作

—快速处理样本。

—通常,回收率高于90%;备有低蛋白质结合的Omega™、Bio-Inert®或GHP滤膜可供选择。

—对应各种截留分子量,备有各种编码以供轻松识别的颜色可供选择。

—结构材料为低结合聚丙烯。

—超声焊接密封,防止侧流或密封不严。

—适用于配合1.5mL试管的标准离心转子。

结构材料

Nanosep离心管

滤材:Omega超滤滤膜(在聚乙烯基质上方的低蛋白质结合改性聚醚砜)

滤出液接收容器:聚丙烯

Nanosep MF(微孔滤膜)离心管

滤材:Bio-Inert或GHP滤膜

滤出液接收容器:聚丙烯

有效过滤面积:0.28 cm2

外形尺寸:总长度(完全盖上盖):4.5厘米(1.8英寸)

处理能力

样本体积:500µL

最终浓缩体积:15µL

滤出液接收容器的容积:500µL

死体积 (滤膜/支撑物):<5µL

工作温度范围:0 – 40℃(32 – 104 °F)

pH值范围:

Nanosep离心管:1-14

Nanosep MF(微孔滤膜)离心管:3-14

离心力:14,000 xg

离心机要求:转子配合1.5mL试管

消毒:产品提交时未经灭菌处理;使用前消毒,可使70%浓度的乙醇过滤。

性能

选择正确的MWCO

MWCO是根据具有已知分子量(以千道尔顿为单位)的溶质保持>90%的能力而给出的额定值。表1给出了某些溶质中不同的MWCO膜的保持特性。对于蛋白质,建议所选的MWCO值比被保持的溶质的分子量低3到6倍。如果首先要考虑流速的因素,那么应该选择MWCO数值处于该范围下限(3X)的膜;如果保持性是首要因素,那么应该选择MWCO数值处于该范围上限(6X)的膜。

表1

在蛋白质应用中选择MWCO

MWCO 额定的膜孔尺寸 生物分子尺寸 生物分子的分子量
1K 3K-10K
3K 10K-20K
5K 15K-30K
10K 30K-90K
30K 90K-180K
50K 5nm 15-30nm 150K-300K
100K 10nm 30-90nm 300K-900K
300K 35nm 90-200nm 900K-1800K

订购信息

带有Omega膜的Nanosep离心管

编号 说明 包装
OD003C33 3K,灰色 24个/包装
OD003C34 3K,灰色 100个/包装
OD003C35 3K,灰色 500pkg
OD010C33 10K,蓝色 24个/包装
OD010C34 10K,蓝色 100个/包装
OD010C35 10K,蓝色 500个/包装
OD030C33 30K,红色 24个/包装
OD030C34 30K,红色 100个/包装
OD030C35 30K,红色 500个/包装
0D100C33 100K,无色 24个/包装
0D100C34 100K,无色 100个/包装
0D100C35 100K,无色 500个/包装
0D300C33 300K,橙色 24个/包装
0D300C34 300K,橙色 100个/包装
0D300C35 300K,橙色 500个/包装

带有Bio-Inert®滤膜的Nanosep MF离心管

部件号 说明 包装
ODM02C33 0.2µm,浅绿色 24个/包装
ODM02C34 0.2µm,浅绿色 100个/包装
ODM02C35 0.2µm,浅绿色 500个/包装
ODM45C33 0.45µm,野草莓色 24个/包装
ODM45C34 0.45µm,野草莓色 100个/包装
ODM45C35 0.45µm,野草莓色 500个/包装

带有GHP膜的Nanasep MF离心管

部件号 说明 包装
ODGHPC34 0.45µm,无色 100个/包装
ODGHPC35 0.45µm,无色 500个/包装
pall 超滤离心管 Nanosep超滤离心管OD100C33、OD100C33、OD030C34、OD010C34

Nanosep® & Nanosep MF超滤离心管
仅需5到15分钟,即可对50到500μL样本进行简便、可靠的浓缩与脱盐处理
— 快速处理样本。
— 通常,回收率高于90%;备有低蛋白质结合的Omega™、Bio-Inert®或GHP滤膜可供选择。
— 对应各种截留分子量,备有各种编码以供轻松识别的颜色可供选择。
— 结构材料为低结合聚丙烯。
— 超声焊接密封,防止侧流或密封不严。
— 适用于配合1.5mL试管的标准离心转子。
应用
— 寡核苷酸、DNA和RNA的浓缩、纯化及脱盐。
— 纯化标记和PCR反应。
— 从琼脂糖凝胶切片中分离DNA。
— 从丙烯酰胺凝胶中分离寡核苷酸和RNA。
— 如果要求去除引物,无论分子量大小为何,浓缩PCR产物时,都必须使用100K离心管。
处理能力
最大样本体积:500μL
最终浓缩体积:15μL
滤出液接收容器的容积:500μL
死体积 (滤膜/支撑物):<5μL
工作温度范围
0 – 40℃(32 – 104 °F)

pH值范围
Nanosep离心管:1-14
Nanosep MF(微孔滤膜)离心管:3-14
最大离心力
14,000 xg
离心机要求
转子配合1.5mL试管
消毒
产品提交时未经灭菌处理;使用前消毒,可使70%浓度的乙醇过滤。
订购信息:
1Nanosep离心浓缩管,Omega
产品编号
说明
包装
OD003C33
3K,灰色
24个/包装
OD003C34
3K,灰色
100个/包装
OD003C35
3K,灰色
500个/包装
OD010C33
10K,蓝色
24个/包装
OD010C34
10K,蓝色
100个/包装
OD010C35
10K,蓝色
500个/包装
OD030C33
30K,红色
24个/包装
OD030C34
30K,红色
100个/包装
OD030C35
30K,红色
500个/包装
OD100C33
100K,透明
24个/包装
OD100C34
100K,透明
100个/包装
OD100C35
100K,透明
500个/包装
OD300C33
300K,橙色
24个/包装
OD300C34
300K,橙色
100个/包装
OD300C35
300K,橙色
500个/包装
2Nanosep离心浓缩管,生物惰性膜
产品编号
说明
包装
ODM02C33
0.2um,浅绿色
24个/包装
ODM02C34
0.2um,浅绿色
100个/包装
ODM02C35
0.2um,浅绿色
500个/包装
ODM45C33
0.45um,野草莓色
24个/包装
ODM45C34
0.45um,野草莓色
100个/包装
ODM45C35
0.45um,野草莓色
500个/包装
3Nanosep离心浓缩管,GHP
产品编号
说明
包装
ODGHPC34
0.45um,无色
100个/包装
ODGHPC35
0.45um,无色
500个/包装

抗躁狂药


产品编号 产品名称 产品规格 产品等级 产品价格
122-01132 Lithium Carbonate 碳酸锂 25g
124-01131 Lithium Carbonate 碳酸锂 100g
126-01135 Lithium Carbonate 碳酸锂 250g

抗躁狂药


◆碳酸锂(Lithium Carbonate)


CAS No. 554-13-2

Li2CO3=73.89

纯度:99.0+%(Titration)(干燥后)

可溶性溶剂:稀盐酸

用途(作用):作用于多数中枢神经系统的神经传递物质和受体。

Li2CO3


相关资料详情请查看:http:///pdf/show/80.html