鸟苷酸激酶[原核生物] Guanylate kinase (prokaryote) 货号:E-GMPK Megazyme中文站

鸟苷酸激酶[原核生物]

英文名:Guanylate kinase (prokaryote)

货号:E-GMPK

规格:500 Units

High purity recombinant Guanylate kinase (prokaryote) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 2.7.4.8

Recombinant from a prokaryote. 
In 3.2 M ammonium sulphate.

Specific activity: 568.4 U/mg (25oC, pH 7.6).

Stable at 4oC for > 2 years.

暂无问题解答

暂无视频

抗突触素抗体[ ye269 ](ab32127)

种属反应性

与反应:小鼠、大鼠、驴、人预测可用于:抗突触素抗体[ ye269 ](ab32127)
  • 应用 AB评论 说明 WB 1 / 20000 – 1 / 100000。检测到的频带的约34 kDa(预测分子量:34 kDa)可阻断。突触体素肽(ab189853) 国际商会/如果 1 / 50 – 1 / 250。

     

    免疫组化法 用于测定浓度依赖性。 ihc-p 1 / 400 – 1 / 800。进行热介导抗原修复免疫组化染色协议开始之前。

    见协议(链路:http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol)。

  • 靶标

    Harlan teklad官网动物饲料 Primate diets 灵长类饮食 国产动物饲料定制

    Harlan teklad官网动物饲料 Primate diets 灵长类饮食 国产动物饲料定制

    特克拉德灵长类饮食是固定的配方饮食,以提供适当的营养平衡所需的新世界和旧世界灵长类动物的所有品种和年龄。饮食是为了提供统一的实验和生产结果而制定的。

    植物、牛奶和鱼类蛋白质来源的结合提供了必需氨基酸的适当数量和平衡,以维持研究灵长类在其整个生命周期和研究功能中的物理状况。

    高质量的脂肪来源提供了生长、维持和繁殖所需的必需脂肪酸的平衡。这些脂肪来源也提供了一种高可消化的能源。

    特克拉德灵长类饮食强化维生素和矿物质,包括维生素D 3,以适当的骨发育和维生素C预防坏血病和贫血。稳定维生素C(L-抗坏血酸-2-聚磷酸)用于延长保质期.饮食是营养完整和平衡的,因此额外的饮食补充剂是不必要的。

    特克拉德灵长类食物被挤压出来,以帮助分解淀粉,提高消化率和适口性。喂食高可消化的膨化食物会产生坚固的大便,这有助于减少清洗时间。

    产品
    特克拉德灵长类食物 不可蒸压式
    全球20%蛋白质灵长类食物 2050
    特克乐全球25%蛋白质灵长类食物 2055
    高纤维灵长类食物 7195
    15%猴子饮食 8714
    15/10高纤维猴子饮食 8788
    新世界灵长类食物 8794
    并不是所有的产品都是在当地库存的,可能需要延长交货期和额外的费用。
    许多饮食都是由符合产品代码的“C”指定的认证格式提供的。当饮食被认证时,一个有代表性的样本会被测试一组污染物。如果没有经过认证的库存,可以根据要求提供认证。可适用最低订货量和附加费用。
    折叠所有标签

    没有一个确定的点,人们能够预测什么时候一个特定的饮食会变质或变得缺乏一个或多个营养。六个月保质期的共同准则是以北美长期的实践为基础的。在欧洲和亚洲,当地做法和监管监督方面的差异导致特克拉德标准天然成分饮食被例行使用到9个月,有时甚至在生产后12个月。这种实践经验,随着时间的推移,再加上文献支持和维生素测试,让我们相信,这些饮食继续支持动物的健康和研究的完整性,至少在9个月后生产。如果你不确定当地的政策,请向你的机构寻求指导。

    建议的储存条件:

    • 凉爽和干燥;在华氏70度或以下,湿度最好在50%以下,但高达65%是可以接受的。
    • 清洁无害虫
    • 在原始包装中或容器中,防止连续暴露于光线下,并将空气暴露在最小范围内。

    一般来说,非人类灵长类动物将消耗相当于其体重百分之四的食物,但是有许多因素可以影响日常消费,包括物种、生命阶段、住房和环境条件。少量的水果、蔬菜或坚果可以作为环境丰富的食物。清洁的淡水应始终可用。

    Teklad primate diets are fixed-formula diets formulated to provide the proper balance of nutrients required by new-world and old-world primates of all breeds and ages. Diets are formulated to provide uniform experimental and production results.

    A combination of plant, milk and fish protein sources supplies the proper amounts and balance of essential amino acids necessary to maintain the physical condition of research primates throughout their life cycle and research function.

    High-quality fat sources provide the balance of essential fatty acids required for growth, maintenance, and reproduction. These sources of fat also provide a highly digestible source of energy.

    Teklad primate diets are fortified with vitamin and minerals including vitamin D3 for proper bone development and vitamin C for prevention of scurvy and anemia. Stabilized vitamin C (L-ascorbyl-2-polyphosphate) is used to extend the shelf life. Diets are nutritionally complete and balanced making additional dietary supplements unnecessary.

    Teklad primate Diets are extruded to help break down starches, improving digestibility and palatability. Feeding highly digestible extruded diets results in firm stools, which helps reduce cleaning time.

    Products
    Teklad primate diets Non-autoclavable form
    Teklad global 20% protein primate diet 2050
    Teklad global 25% protein primate diet 2055
    Teklad hi-fiber primate diet 7195
    Teklad 15% monkey diet 8714
    Teklad 15/10 hi-fiber monkey diet 8788
    Teklad new world primate diet 8794
    Not all products are stocked locally; extended lead time and additional fees may apply.
    Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.

    COLLAPSE ALL TABS

    There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. The common guideline of a six month shelf life is based on longstanding practice in North America. In Europe and Asia, differences in local practices and regulatory oversight have led to Teklad standard natural ingredient diets being routinely used out to nine months and sometimes 12 months post-manufacture. This practical experience, along with literature support and vitamin testing over time, gives us confidence that these diets continue to support animal health and study integrity out to at least nine months post-manufacture. Please refer to your institution for guidance if you are unsure of local policies.

    Recommended storage conditions:

    • Cool and dry; at or below 70 degrees fahrenheit with humidity ideally below 50%, but up to 65% is acceptable
    • Clean and free of pests
    • In original packaging or in a container that prevents continuous exposure to light and minimal exposure to air

    In general, nonhuman primates will consume an amount of diet equal to four percent of their body weight but there are many factors that can affect daily consumption including species, life-stage, housing, and environmental conditions. Small amounts of fruits, vegetables, or nuts may be fed as environmental enrichment. Clean fresh water should always be available.

    Ready PCR Mix AMRESCO N806

    名称:Ready PCR Mix

    品牌:AMRESCO

    订货号:N806

    Ready PCR Mix                                                        AMRESCO                                                        N806

    咨询此产品

    产品介绍

    Ready PCR Mix is a PCR master mix that enables reaction set-up through product visualization with a single, easy-to-use solution. With Ready PCR Mix, your laboratory can achieve high quality DNA amplification while slashing time and consumption of supplies and hazardous reagents. Ready PCR Mix can:

    • Reduce PCR reaction set up and post PCR steps
    • Streamline colony screening by eliminating the need for DNA purification
    • Eliminate Ethidium Bromide staining and associated waste disposal expenses


    The Ready PCR Mix solution includes:

    • Extender™ Taq DNA Polymerase
      • High fidelity polymerase blend
      • Efficient amplification of long and challenging templates (To learn more, click on the features tab of the linked page)
    • Reaction Buffer
      • MgCl2
      • dNTPs
    • Density Reagent/Tracking Dye
      • Load reaction aliquots directly onto gel
      • Non-interfering, fast migrating dye
    • Fluorescent DNA Dye
      • Based on AMRESCO\’s non-mutagenic and non-toxic EZ-Vision™ formulation
      • Immediate visualization of amplified product with no post-run staining or destaining
      • Eliminates use of Ethidium Bromide and associated hazardous waste disposal costs
    Ready PCR Mix                                                        AMRESCO                                                        N806

    Efficient Amplification of Purified DNA with Ready PCR Mix

    Figure 1. Single copy targets were amplified from 50ng of pUC19 plasmid (A) or S. aureus genomic DNA (B) for 35 cycles. 10μl of each PCR reaction was directly loaded onto a 1\% agarose gel and visualized with a Syngene HR gel doc system.

     

    Direct Colony Screening with Ready PCR Mix

     

    Figure 2. 10 distinct colonies were suspended into Ready PCR Mix in separate PCR tubes. 5 μl of the PCR reaction mix was plated on gridded LB plates with Kanamycin for 37ºC overnight growth. After 30 PCR cycles products were screened on a 1\% agarose gel. Colonies 4, 8, 9 and 10 contained the desired sequences. Courtesy of Dr. Irene Lee, Case Western Reserve University, Cleveland, OH

     

    DNA Amplification by Ready PCR Mix Outperforms a Competitor Master Mix

    Figure 3. Amplification products from AMRESCO Ready PCR Mix (Lanes 5-8) and a competitor master mix (Lanes 1-4) were resolved on a TAE/1.5\%  agarose gel containing Ethidium Bromide. Ethidium Bromide was included in the gel to visualize the products from the competitor master mix which lacks a DNA dye. Lanes 1, 2, 3:  Aliquots (5 µl)  from triplicate reactions with competitor master mix. Lanes  5,6,7: Aliquots (5 µl)  from triplicate reactions with AMRESCO Ready PCR mix. Lanes 4 & 8: Non-template controls (5 µl) from competitor master mix and AMRESCO Ready PCR mix respectively. Data courtesy of Norgen Biotek Corporation.

    Enduro GDS 凝胶成像系统

    品牌:美国Labnet

    订货号:GDS-1302-230V

    产品介绍

    Labnet Enduro GDS 是一款采用500万像素科研级别CCD摄像头的高清凝胶成像系统,相对于目前市场上主流百万级别的产品,它具有无可挑剔的成像效果,图像极致清晰细腻。Enduro GDS凝胶成像系统采用了预对焦模式,不需要你更多的调整,即可以获得清晰图像。

     

    主要特点:

    ★应用灵活广泛,支持各种DNA和蛋白质凝胶的荧光、可见光、染色等成像

    500万像素原生CCD摄像头,成像效果极致清晰细腻

    ★整合了紫外透射仪、白光灯等所有功能部件

    ★外形小巧,仅14英寸*12.5英寸

    ★具有图像预览功能

    ★视野广阔,高达20cm*24cm

    ★图像获取简单,一键获取,一目了然

    ★不需安装,拆箱即插即用

    主要参数:

    摄像头

    500万像素原生CCD高清摄像头

    ★简单USB安装

    ★快速刷新率,获取实时影像

    ★高质量图像,成就理想下游分析

    镜头

    预对焦功能,同时可以手动调节

    视野

    20cm*24cm

    机箱

    紫外投射光源,双波长(302/365nm),双强度紫外观察窗口

    滤光器

    590滤光器

    应用

    荧光、可见光成像,凝胶成像

    认证标准

    CE

    尺寸

    14英寸*12.5英寸

    适用电脑

    台式或笔记本

    订货号

    GDS-1302-230V

    • 上海金畔生物科技有限公司

      文章号:290-290