磷酸葡糖异构酶[枯草杆菌] Phosphoglucose isomerase (Bacillus subtilis) 货号:E-PGIBS-50KU Megazyme中文站

磷酸葡糖异构酶[枯草杆菌]

英文名:Phosphoglucose isomerase (Bacillus subtilis)

货号:E-PGIBS-50KU

规格:50000 units

High purity Phosphoglucose isomerase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 5.3.1.9

From Bacillus subtilis. Electrophoretically homogeneous (MW 51,500). Several bands on isoelectric focusing (pI 4.8-5.0).
In 3.2 M ammonium sulphate.

Specific activity: 250 U/mg of protein (25oC, pH 7.6) or 483 U/mg of protein (40oC, pH 7.6).

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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2-硝基苯基β-木糖苷 2-Nitrophenyl-β-xylobioside 货号:O-ONPX2-5MG Megazyme中文站

2-硝基苯基β-木糖苷

英文名:2-Nitrophenyl-β-xylobioside

货号:O-ONPX2-5MG

规格:5 mg

CAS: 157956-98-4
Molecular Formula: C16H21NO11
Molecular Weight: 403.3
Purity: 98%

High purity 2-Nitrophenyl-β-xylobioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for assay of xylanase and xylosidase activity. 

Data booklets for each pack size are located in the Documentation tab.

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内切纤维素酶检测试剂盒[CELLG5方法] Cellulase Assay Kit (CELLG5 Method) 货号:K-CELLG5-2V Megazyme中文站

内切纤维素酶检测试剂盒[CELLG5方法]

英文名:Cellulase Assay Kit (CELLG5 Method)

货号:K-CELLG5-2V

规格:60 / 120 assays (manual) / 240 (auto-analyser)

The CELLG5 assay reagent for the measurement of
endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the
β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

 

Colourimetric method for the determination of
endo
-1,4-β-glucanase (cellulase) in enzyme preparations and fermentation products

Principle:
(endo-1,4-β-glucanase)
(1) 3-Ketobutylidene-G5-β-PNP + H2O → Blocked-GX + G(5-X)-β-PNP

(thermostable β-glucosidase)
(2) G(5-X)-β-PNP + H2O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:
K-CELLG5-4V 120 / 240 assays (manual) / 480 (auto-analyser)
or
K-CELLG5-2V 60 / 120 assays (manual) / 240 (auto-analyser)

Method: Spectrophotometric at 400 nm
Total assay time: 10 min
Detection limit: 3.5 x 10-4 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition: Novel method

Advantages

  • Very cost effective
  • All reagents stable for > 4 years
  • Completely specific for cellulase (endo-1,4-glucanase)
  • Generally applicable and highly sensitive
  • Simple format. Well suited to automation
  • Standard included

 

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Megazyme品牌介绍

Megazyme品牌介绍–上海金畔

1. Megazyme品牌介绍
Megazyme公司1988年成立于爱尔兰,是全球知名的酶及酶法分析试剂盒的生产厂家,该公司目前提供了超过70种诊断试剂盒和超过300种其他试剂和底物用于检测谷物,食品,饲料,发酵,乳制品和葡萄酒行业。其大多数产品都被AOAC(Association of Official Analytical Chemists)、AACC(American Association of Cereal Chemists)、ICC(International Association for Cereal Science and Technology)、 EBC(European Brewing Convention)、RACI(Royal Australian Chemical Institute)国际组织采用。其中欧洲检测果汁及葡萄酒等发酵产品基本都采用该公司产品。
2. Megazyme重点产品
酶及酶法分析试剂盒
检测果汁及葡萄酒等发酵产品
3. Megazyme官网
http://secure.megazyme.com
4. 金畔生物代理Megazyme品牌联系方式

上海金畔生物科技有限公司
地 址: 上海市浦东新区东靖路699弄36栋701室
邮 编: 201208
qq: 2743691513
固话总机:021-50837765
订货热线:15221999938
网 址: www.jinpanbio.com
Email:sales@jinpanbio.com

壳聚糖酶半定量检测底物 Chitozyme – 200 Tablets 货号:T-CHZ-200T Megazyme中文站

壳聚糖酶半定量检测底物

英文名:Chitozyme – 200 Tablets

货号:T-CHZ-200T

规格:200 Tablets

High purity dyed and crosslinked Chitozyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

Substrate for the semi-quantitative assay of chitosanase.

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木聚糖酶AX检测底物 Xylazyme AX (60mg) 1000 Tablets 货号:T-XAX-1000T Megazyme中文站

木聚糖酶AX检测底物

英文名:Xylazyme AX (60mg) 1000 Tablets

货号:T-XAX-1000T

规格:1000 Tablets

High purity dyed and crosslinked Xylazyme AX (60 mg tablets) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of endo-1,4-ß-D-xylanase. Containing AZCL-arabinoxylan (wheat).

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半乳聚糖酶特殊检测底物 Azo-Galactan (Potato) 4g 货号:S-AGALP Megazyme中文站

半乳聚糖酶特殊检测底物

英文名:Azo-Galactan (Potato) 4g

货号:S-AGALP

规格:4 grams

Substrate for the specific measurement of endo-1,4-ß-D-galactanase. Prepared from potato galactan pre-treated with a-L-arabinofuranosidase to reduce arabinose content.

半乳聚糖酶特殊检测底物

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淀粉葡萄糖苷酶(黑曲霉)游离甘油 Amyloglucosidase (Aspergillus niger) Glycerol Free 货号:E-AMGDFNG Megazyme中文站

淀粉葡萄糖苷酶(黑曲霉)游离甘油

英文名:Amyloglucosidase (Aspergillus niger) Glycerol Free

货号:E-AMGDFNG

规格:50 mL

High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

EC 3.2.1.3 
CAZy Family: GH15
CAS: 9032-08-0 

glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase

From Aspergillus niger.
In buffered solution.

This enzyme is recommended for use in Total Dietary Fibre analytical procedures and the Megazyme Total Starch test method.

The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method). 

Specific activity: ~ 36 U/mg (40oC, pH 4.5 on soluble starch); ~ 400 U/mL on p-nitrophenyl β-maltoside; ~ 6,520 U/mL on soluble starch.

Stability: > 4 years 4oC.

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阿魏酸酯酶[瘤胃微生物] Feruloyl esterase (rumen microorganism) 货号:E-FAERU Megazyme中文站

阿魏酸酯酶[瘤胃微生物]

英文名:Feruloyl esterase (rumen microorganism)

货号:E-FAERU

规格:1000 Units

High purity recombinant Feruloyl Esterase (Rumen Microorganism) for use in research, biochemical enzyme assays andin vitro diagnostic analysis.

EC 3.1.1.73

Recombinant. Feruloyl esterase from a rumen microorganism. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 40 U/mg on ethyl ferulate at pH 6.5 and 40oC.

Stability: > 2 years at 4oC.

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阿拉伯聚糖酶检测片剂 Arabinazyme – 200 Tablets 货号:T-ARZ-200T Megazyme中文站

阿拉伯聚糖酶检测片剂

英文名:Arabinazyme – 200 Tablets

货号:T-ARZ-200T

规格:200 Tablets

High purity dyed and crosslinked Arabinazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of endo-1,5-α-L-arabinanase. Containing AZCL-Debranched arabinan.

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α-D-甘露糖苷酶(多形拟杆菌) α-D-Mannosidase (Bacteroides thetaiotaomicron) 货号:E-AMANBT Megazyme中文站

α-D-甘露糖苷酶(多形拟杆菌)

英文名:α-D-Mannosidase (Bacteroides thetaiotaomicron)

货号:E-AMANBT

规格:10 Units

High purity α-D-Mannosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.24
CAZy Family: GH92
CAS: 9025-42-7

alpha-mannosidase; alpha-D-mannoside mannohydrolase

Recombinant. From Bacteroides thetaiotaomicron.
In 3.2 M ammonium sulphate.
Supplied at ~ 100 U/mL. 

Specific activity: ~ 8 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.5 and 40oC.

Stability: > 4 years at 4oC.

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纤维素酶[1,4-D-葡聚糖内切酶 [长枝木霉] Cellulase (endo-1,4-β-D-glucanase) (Trichoderma longibrachiatum) 货号:E-CELTR Megazyme中文站

纤维素酶[1,4-D-葡聚糖内切酶 [长枝木霉]

英文名:Cellulase (endo-1,4-β-D-glucanase) (Trichoderma longibrachiatum)

货号:E-CELTR

规格:1000 Units

High purity Cellulase (endo-1,4-β-D-glucanase) (T. Longibrachiatum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.4

From Trichoderma longibrachiatum. Electrophoretically homogeneous. 
In 3.2 M ammonium sulphate.

Specific activity: ca. ~ 70 U/mg (40oC, pH 4.5, CM-cellulose 4M as substrate). 

Stable at 4oC for > 4 years.

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麦芽粉中葡聚糖酶检测底物 Azo-Barley Glucan 100ml 货号:S-ABG100 Megazyme中文站

麦芽粉中葡聚糖酶检测底物

英文名:Azo-Barley Glucan 100ml

货号:S-ABG100

规格:100 mL

Highly purified, low viscosity barley 1,3:1,4-ß-D-glucan dyed with Remazolbrilliant Blue R dye. Recommended substrate for the measurement of ß-glucanase in malt flour.

麦芽粉中葡聚糖酶检测底物

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内切纤维素酶检测试剂盒[CELLG5方法] Cellulase Assay Kit (CELLG5 Method) 货号:K-CELLG5-4V Megazyme中文站

内切纤维素酶检测试剂盒[CELLG5方法]

英文名:Cellulase Assay Kit (CELLG5 Method)

货号:K-CELLG5-4V

规格:120 / 240 assays (manual) / 480 (auto-analyser)

The CELLG5 assay reagent for the measurement of
endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the
β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

 

Colourimetric method for the determination of
endo
-1,4-β-glucanase (cellulase) in enzyme preparations and fermentation products

Principle:
(endo-1,4-β-glucanase)
(1) 3-Ketobutylidene-G5-β-PNP + H2O → Blocked-GX + G(5-X)-β-PNP

(thermostable β-glucosidase)
(2) G(5-X)-β-PNP + H2O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:
K-CELLG5-4V 120 / 240 assays (manual) / 480 (auto-analyser)
or
K-CELLG5-2V 60 / 120 assays (manual) / 240 (auto-analyser)

Method: Spectrophotometric at 400 nm
Total assay time: 10 min
Detection limit: 3.5 x 10-4 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition: Novel method

Advantages

  • Very cost effective
  • All reagents stable for > 4 years
  • Completely specific for cellulase (endo-1,4-glucanase)
  • Generally applicable and highly sensitive
  • Simple format. Well suited to automation
  • Standard included

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受阻4-甲基β-纤维三糖 Blocked 4-methylumbelliferyl-β-cellotrioside 货号:O-B4MUG3 Megazyme中文站

受阻4-甲基β-纤维三糖

英文名:Blocked 4-methylumbelliferyl-β-cellotrioside

货号:O-B4MUG3

规格: 5 mL of 10mM substrate in DMSO (37.5 mg)

CAS: 1621689-96-0
Molecular Formula: C35H42O18
Molecular Weight: 750.7
Purity: > 95%
High purity Blocked 4-methylumbelliferyl-β-cellotrioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
A potential substrate for the measurement of cellulase (endo-1,4-β-glucanase). This product requires the use of ancillary β-glucosidase (eg. E-BGOSPC from Megazyme).
Note: This product will be discontinued in 2017. It is replaced by the Cellafluor Reagent (R-CELLFLR) which also contains the required β-glucosidase.

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L-精氨酸/尿素/氨检测试剂盒[快速] L-Arginine/Urea/Ammonia (Rapid) Assay Kit 货号:K-LARGE Megazyme中文站

L-精氨酸/尿素/氨检测试剂盒[快速]

英文名:L-Arginine/Urea/Ammonia (Rapid) Assay Kit

货号:K-LARGE

规格:150 assays – 50 of each component

For the specific and rapid assay of L-arginine, urea and ammonia in grape juice/must and wine. Content:150 assays – 50 of each component

UV-method for the determination of L-Arginine, Urea and Ammonia 
in grape juice, must and wine

Principle:
                            (microbial glutamate dehydrogenase)
(1) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP+ + 
                                                                                           H2O
                     (urease)
(2) Urea + H2O → 2 NH3 + CO2

                              (arginase)
(3) L-Arginine + H2O → urea + ornithine

Kit size:                             50 assays
Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 20 min [ammonia (2 min), urea (6 min),
                                          L-arginine (7 min)] Detection limit:                  0.07 mg/L (ammonia)
                                           0.13 mg/L (urea)
                                           0.37 mg/L (L-arginine)
Application examples:
Grape juice, wine must, wine and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:          Improved method

Advantages

  • Improved assay format
     
  • Very rapid reactions due to use of uninhibited glutamate dehydrogenase
     
  • All enzymes supplied as stabilised suspensions
     
  • Very competitive price (cost per test)
     
  • All reagents stable for > 2 years after preparation
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Extended cofactors stability

 

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1,4-b甘露聚糖内切酶[芽孢杆菌] endo-1,4-?-Mannanase (C.japonicus) 货号:E-BMACJ Megazyme中文站

1,4-b甘露聚糖内切酶[芽孢杆菌]

英文名:endo-1,4-?-Mannanase (C.japonicus)

货号:E-BMACJ

规格:10000 Units

High purity recombinant endo-1,4 beta-Mannanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.78 
CAZy Family: GH26

Recombinant. From Cellvibrio japonicus.
In 3.2 M ammonium sulphate.

Specific activity: ~ 420 U/mg (40oC, pH 7.0 on carob galactomannan); ~ 640 U/mg (50oC, pH 7.0 on carob galactomannan).

Store at 4oC.

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麦芽淀粉酶检测试剂盒 Malt Amylase Assay Kit 货号:K-MALTA Megazyme中文站

麦芽淀粉酶检测试剂盒

英文名:Malt Amylase Assay Kit

货号:K-MALTA

规格:100 assays (50 of each) per kit

The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.

Colourimetric method for the determination of α-Amylase and
β-Amylase in cereal grains, malt, food, beverages and
fermentation products

Principle:
(1) α-Amylase is measured using the “Ceralpha” Method as used
in K-CERA

(2) β-Amylase is measured using the “Betamyl-3” Method as used
in K-BETA3

Kit size: 50 assays of each
Method: Spectrophotometric at 400 nm
Reaction time: ~ 20 min (Ceralpha Method)
~ 10 min (Betamyl-3 Method)
Detection limit: 0.05 U/mL
Application examples:
Cereal flours, malts, fermentation broths and other materials
Method recognition:
“Ceralpha” Method: AOAC (Method 2002.01), AACC (Method
22-02.01), ICC (Standard No. 303), RACI
(Standard Method) and CCFRA (Flour Testing
Working Group Method 0018)
“Betamyl-3” Method: RACI (Standard Method)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years as supplied
  • Only enzymatic kit available (Beta-Amylase)
  • Very specific
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

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Alpha葡[萄]糖苷酶 [转葡糖苷酶][黑曲霉] Alpha-Glucosidase(Transglucosidase) 货号:E-TRNGL Megazyme中文站

Alpha葡[萄]糖苷酶 [转葡糖苷酶][黑曲霉]

英文名:Alpha-Glucosidase(Transglucosidase)

货号:E-TRNGL

规格:2000 Units

Alpha葡[萄]糖苷酶 [转葡糖苷酶][黑曲霉]

EC 3.2.1.20
CAZY Family: GH31
From A. niger. In 3.2 M ammonium sulphate.
Specific activity: 70 U/mg (40°C, pH 4.5, maltose as substrate). 2.5 U/mg (phenyl α-glucoside as substrate).
Contaminants: α-amylase < 0.001%; amyloglucosidase < 0.01%.
Stable at 4°C for > 4 years.

 

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