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Glucose Dehydrogenase (FADGDH-AA) 葡萄糖脱氢酶
- 临床检测用酶
The enzyme is useful for the determination of D-glucose in clinical analysis and self-monitoring blood glucose meters.
| 由来 | recombinant A. sojae |
|---|---|
| 系统名称 |
D-Glucose : acceptor 1-oxidoreductase |
| EC编号 | 1.1.5.9 |
| 反应式 |
D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
规格
| Appearance | yellow lyophilizate |
|---|---|
| Activity | ≧500 U/mg |
| Contaminants | NAD glucose dehydrogenase ≺1.0×10-2％ |
| Hexokinase ≺1.0×10-2 U/U% | |
| α-Glucosidase ≺1.0×10-2 U/U% | |
| β-Glucosidase ≺1.0×10-2 U/U% | |
| Storage condition | below -20℃ protected from light |
特性
| Molecular weight | ca. 90 kDa (gel filtration) |
|---|---|
| Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
| Michaelis constant | 9.5×10-2M (D-glucose) |
| pH Optimum | 7.0–7.5 |
| pH Stability | 2.5–7.5 |
| Optimum temperature | 40–50℃ |
| Thermal stability | below 50℃ |
| Inhibitors | Ag+ |
| Specificity | D-glucose (100), maltose (≺1), |
| D-xylose (≺1), D-galactose (≺1) |
适用类型
The enzyme is useful for the determination of D-glucose in clinical analysis and self-monitoring blood glucose meters.
参考文献
Satake, R. et al., J Biosci Bioeng., 120, 498–503 (2015)
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