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The enzyme is useful for the determination of uric acid in clinical analysis.
| 由来 |
recombinant E. coli |
| 系统名称 |
Urate : oxygen oxidoreductase
|
| EC编号 |
1.7.3.3 |
| 反应式 |
Urate + H2O + O2 →→→ Unidentified products + H2O2
↓
CO2 + Allantoin (racemic mixture)
|
规格
| Appearance |
light brownish lyophilizate |
|
| Activity |
≧4.0 U/mg |
|
| Contaminants |
Catalase ≦1.0 U/U% |
|
| Stabilizer |
sucrose, citrate, ethylenediaminetetraacetic acid (EDTA) |
|
| Storage condition |
below -20℃ |
|
特性
| Molecular weight |
ca. 90 kDa (gel filtration) |
| Structure |
2 subunits of 35 kDa (SDS-PAGE) |
| Michaelis constant |
1.1×10-5M (uric acid) |
| pH Optimum |
8.5 |
| pH Stability |
7.0–11.0 |
| Optimum temperature |
45℃ |
| Thermal stability |
below 55℃ |
| Stability (liquid form) |
stable at 37℃ for at least ten days |
| Stability (powder form) |
stable at 30℃ at least three weeks |
| Inhibitors |
Hg2+，Ag+ |
适用类型
The enzyme is useful for the determination of uric acid in clinical analysis.
参考文献
Koyama, Y. et al., J. Biochem., 120, 969–973 (1996).
