木聚糖酶检测试剂盒 Xylanase Assay Kit (Azo-Wax) 货号:K-AZOWAX Megazyme中文站

木聚糖酶检测试剂盒

英文名:Xylanase Assay Kit (Azo-Wax)

货号:K-AZOWAX

规格:200 assays per kit

产品名称:木聚糖酶检测试剂盒
英文名称:Xylanase Assay Kit (Azo-Wax)

型号规格:200次
品牌:爱尔兰Megaeyme公司

自然界中,D-木糖以多糖形式如木聚糖,阿拉伯糖基木聚糖,GAX(glucuronoarabinoxylan),木葡聚糖和XGA(xylogalacturonan)存在。一些海藻中也存在交联D-木糖,据认定一些相近多糖构成洋车前子胶的骨架。番石榴,梨,黑莓,罗甘莓,悬钩子,芦荟维拉胶,褐藻,紫锥菊,乳香树,花椰菜,菠菜,茄子,豌豆,青豆,秋葵,卷心菜和谷物中存在游离的D-木糖。D-木糖用于小肠疾病诊断中的吸收实验,如食品中营养,维生素和矿物质吸收障碍。D-木糖可以被小肠正常轻松地吸收,当患有这些疾病时,D-木糖不能被吸收,血液和尿中的含量很低。D-木糖检测可以帮助确定儿童无法增重的原因,尤其是那些进食足够的儿童。如果在多糖中, D-木糖与其他糖类的比例已知,就可以通过酸水解测定D-木糖的浓度进而定量多糖。木聚糖是一些多糖的主要部分,这些多糖水解后的可发酵糖类可用于生产生物燃料。

原理

D-木糖的α-和β-变旋异构形式互换由木糖变旋酶(XMR)催化(1)。

在pH7.5下,β-D-木糖由β-木糖脱氢酶(β-XDH)催化,被NAD+氧化为D-木糖酸。

The Xylanase (Azo-Wax) test kit is suitable for the measurement and analysis of endo-1,4-β-D-xylanase in enzyme preparations, bread improver mixtures and animal feeds. Containing Azo-wheat arabinoxylan and a Trichoderma sp. xylanase control.

Colourimetric method for the determination of Xylanase in feed,
foodstuffs and other materials

Principle:
(β-xylanase)
(1) Azo-WAX + H2O → Azo-WAX fragments
(insoluble in aqueous alcohol) (soluble in aqueous alcohol)

Kit size: 200 assays
Method: Based on use of Azo-WAX reagent (590 nm)
Total assay time: ~ 45 min
Detection limit: 0.2 U/mL of assay solution
Application examples:
Animal feeds, enzyme preparations, bread improver mixtures and
other materials
Method recognition:
Used widely in the feed industry

Advantages

  • Very cost effective
  • All reagents stable for > 2 years
  • Only test kit available
  • Simple format
  • Standard included

 

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