Beta葡聚糖[酵母和蘑菇]检测试剂盒 β-Glucan Assay Kit (Yeast & Mushroom) 货号:K-YBGL Megazyme中文站

Beta葡聚糖[酵母和蘑菇]检测试剂盒

英文名: β-Glucan Assay Kit (Yeast & Mushroom)

货号:K-YBGL

规格:100 assays per kit

For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit

Colourimetric method for the determination of Yeast and 
Mushroom β-Glucan in yeast, mushroom, foodstuffs and 
other materials

Principle:
                                                              (conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O → 
                                                                        soluble glucan

                         (1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)

                  (exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H2O → D-glucose

                              (glucose oxidase)
(4) D-Glucose + H2O + O2 → D-gluconate + H2O2

                                                                                (peroxidase)
(5) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
                                                                        quinoneimine + 4H2O

                       (amyloglucosidase)
(6) α-Glucan + H2O    →    D-glucose

Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:              ~ 100 min
Detection limit:                 1-100% of sample weight
Application examples:
Yeast preparations, mushroom preparations and other materials
Method recognition:        Novel method

Advantages

  • Very cost effective
     
  • All reagents stable for > 12 months after preparation
     
  • Only enzymatic kit available
     
  • Simple format
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included

 

Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

 

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.